CN102177872B - Method for obtaining anti-freeze protein sources of insects - Google Patents
Method for obtaining anti-freeze protein sources of insects Download PDFInfo
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- CN102177872B CN102177872B CN201110091198A CN201110091198A CN102177872B CN 102177872 B CN102177872 B CN 102177872B CN 201110091198 A CN201110091198 A CN 201110091198A CN 201110091198 A CN201110091198 A CN 201110091198A CN 102177872 B CN102177872 B CN 102177872B
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- antifreeze protein
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Abstract
The invention provides a method for obtaining anti-freeze protein sources of insects, which comprises the following steps of: selecting insects which are adapt to survive in cold areas; cultivating the insects, and controlling ambient temperature in one life cycle of the cultivated insects, namely reducing gradually until the ambient temperature is below 0 DEG C and is above the minimum survival temperature; and harvesting the cultivated insects, namely the anti-freeze protein sources of the insects. Compared with the prior art, by the method, the anti-freeze protein sources of the insects can be obtained industrially, and the quality of the obtained anti-freeze protein of the insects is improved.
Description
Technical field
What the present invention relates to is the acquisition methods in a kind of insect antifreeze protein source, belongs to the cultured insect technical field of the essential middle agricultural of human lives.
Background technology
Antifreeze protein be one type with ice crystal affinity is arranged, can combine and suppress the albumen or the glycoprotein of ice-crystal growth with ice crystal.Since the sixties in 20th century, the researcher has found multiple antifreeze protein respectively from fish, insect, plant, fungi and bacterium.Found insect antifreeze protein activity in recent years generally than higher, therefore came into one's own.The insect antifreeze protein is the interior peptide species that exists of insect bodies on the ground of cold, and the insect on the ground of those colds is exactly insect antifreeze protein source, and their survivals on the ground of cold are exactly the low-temperature protection ability that depends on the insect antifreeze protein.But because said this type insect results are relatively more difficult, can not obtain in a large number, make the insect antifreeze protein also be in conceptual phase.
Summary of the invention
To above-mentioned deficiency, technical problem to be solved by this invention is how to obtain the insect on the ground of cold in a large number, thereby a kind of acquisition methods that makes the insect antifreeze protein get into a kind of insect antifreeze protein source in industry stage is provided.
The acquisition methods in insect antifreeze protein provided by the invention source; It is the insect of choosing the ground existence that seasons oneself to cold earlier; Again said insect is cultured; The temperature that in the life cycle of supporting insect, controls environment progressively reduces more than minimum existence temperature below 0 ℃, gathers in the crops the insect of being supported at last, and these insects are insect antifreeze protein source.
The acquisition methods in insect antifreeze protein provided by the invention source; The insect that has ability will be arranged in an one of which life cycle on the ground of cold; Promptly from ovum, larva, pupa in this process of adult; The temperature of artificial its growing environment of control progressively drops at the bottom of its low temperature resistant utmost point, and keeps a time period.In this case, insect is the decline of adaptive temperature, will in its body, excite antifreeze protein formation, abundant and make antifreeze protein progressively adaptive temperature drop to the limit of supporting one's family, thereby make the antifreeze protein structure in its body that best anti-freezing property arranged.Compared with prior art, the present invention can make the acquisition in insect antifreeze protein source form industrialization, and improves the quality of the insect antifreeze protein that is extracted.
The acquisition methods in insect antifreeze protein provided by the invention source; In breeding process; Earlier insect larvae is carried out the breed under the developmental temperature; Before larvae pupation, environmental temperature is controlled, temperature is progressively descended, continued control temperature descends more than minimum existence temperature below 0 ℃ pupating.
Saidly earlier larva is separated with bait when before larvae pupation, environmental temperature being controlled.
The process that the said temperature that controls environment reduces is, drops to 5 ~ 10 ℃ with 4 ~ 8 ℃ the amplitude of descending every day earlier, descends 0.5 ~ 2 ℃ more than minimum existence temperature below 0 ℃ every day in the insect larvae back of all pupating.
Said before results, earlier insect was kept 2 ~ 15 days under the cooling final temperature, farthest to transform the antifreeze protein that produces in its body by insect.
Embodiment
1, the young maggot of selecting the northeast fly begins to raise under 24 ~ 28 ℃ of room temperatures, and beginning is controlled room temperature and descend 3 ℃ every day, and control room temperature descend 1 ℃ up to dropping to-5 ℃ of results every day after 10 ℃.
2, yellow mealworm is supported in choosing, raise closely under 20 ~ 26 ℃ of the room temperatures and pupate the stage, with the worm totality from and to the worm temperature that controls environment; Beginning to control every day room temperature descends 5 ℃; Larva all pupates during to 6 ℃ of left and right sides, controls room temperature then every day and descends 0.5 ℃ up to dropping to-18 ℃, keeps 10 days results.
3, Chi Chijia is supported in choosing; Under room temperature, raise and get larva in the time of will pupating under the temperature control environment, every day is controlled 7 ℃ of room temperature declines in beginning, moves in the refrigerating plant after all pupating to 5 ℃ of left and right sides larvas; Control temperature every day and descend 1 ℃, keep 4 days results up to dropping to-16 ℃.
4, fly is supported in choosing; Under room temperature, raise and get larva in the time of will pupating under the temperature control environment, every day is controlled 4 ℃ of room temperature declines in beginning, moves in the refrigerating plant after all pupating to 8 ℃ of left and right sides larvas; Control temperature every day and descend 1.5 ℃, keep 5 days results up to dropping to-20 ℃.
Claims (5)
1. the acquisition methods in an insect antifreeze protein source; It is characterized in that choosing earlier the insect of the ground existence that seasons oneself to cold; Again said insect is cultured; The temperature that in the life cycle of supporting insect, controls environment progressively reduces more than minimum existence temperature below 0 ℃, gathers in the crops the insect of being supported at last, and these insects are insect antifreeze protein source.
2. the acquisition methods in insect antifreeze protein as claimed in claim 1 source; It is characterized in that in breeding process; Earlier insect larvae is carried out the breed under the developmental temperature; Before larvae pupation, environmental temperature is controlled, temperature is progressively descended, continued control temperature descends more than minimum existence temperature below 0 ℃ pupating.
3. the acquisition methods in insect antifreeze protein as claimed in claim 2 source is characterized in that saidly earlier larva being separated with bait when before larvae pupation, environmental temperature being controlled.
4. the acquisition methods in insect antifreeze protein as claimed in claim 3 source; It is characterized in that the process that the said temperature that controls environment reduces is; Earlier drop to 5～10 ℃, descend 0.5～2 ℃ more than minimum existence temperature below 0 ℃ every day in the insect larvae back of all pupating with 4～8 ℃ the amplitude of descending every day.
5. like the acquisition methods in claim 1 or 2 or 3 or 4 described insect antifreeze protein sources, it is characterized in that before said results, earlier insect was kept 2～15 days under the cooling final temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110091198A CN102177872B (en) | 2011-04-13 | 2011-04-13 | Method for obtaining anti-freeze protein sources of insects |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110091198A CN102177872B (en) | 2011-04-13 | 2011-04-13 | Method for obtaining anti-freeze protein sources of insects |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102177872A CN102177872A (en) | 2011-09-14 |
| CN102177872B true CN102177872B (en) | 2012-10-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110091198A Expired - Fee Related CN102177872B (en) | 2011-04-13 | 2011-04-13 | Method for obtaining anti-freeze protein sources of insects |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110950945B (en) * | 2019-12-31 | 2021-08-31 | 石河子大学 | Antifreeze protein of Hepialus insect and coding gene and application thereof |
-
2011
- 2011-04-13 CN CN201110091198A patent/CN102177872B/en not_active Expired - Fee Related
Non-Patent Citations (8)
| Title |
|---|
| 冯从经等.抗冻蛋白研究进展.《江苏农业学报》.2007,第23卷(第5期),481-486. |
| 抗冻蛋白研究进展;冯从经等;《江苏农业学报》;20071231;第23卷(第5期);481-486 * |
| 昆虫对低温的适应——抗冻蛋白研究进展;景晓红等;《昆虫学报》;20021231;第45卷(第5期);679-683 * |
| 昆虫抗冻蛋白的研究进展;钟鸣等;《生物技术通报》;20101231(第10期);8-14 * |
| 昆虫抗冻蛋白的结构与生物学特性研究;马纪等;《生物技术通报》;20061231(第5期);37-44 * |
| 景晓红等.昆虫对低温的适应——抗冻蛋白研究进展.《昆虫学报》.2002,第45卷(第5期),679-683. |
| 钟鸣等.昆虫抗冻蛋白的研究进展.《生物技术通报》.2010,(第10期),8-14. |
| 马纪等.昆虫抗冻蛋白的结构与生物学特性研究.《生物技术通报》.2006,(第5期),37-44. |
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| CN102177872A (en) | 2011-09-14 |
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Inventor after: Xu Gongda Inventor after: Han Yongmiao Inventor before: Xu Gongda |
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Free format text: CORRECT: INVENTOR; FROM: XU GONGDA TO: XU GONGDA HAN YONGMIAO |
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Granted publication date: 20121003 Termination date: 20140413 |