CN107455343A - Predatory Mites are cut open the belly the acquisition methods of ovum - Google Patents
Predatory Mites are cut open the belly the acquisition methods of ovum Download PDFInfo
- Publication number
- CN107455343A CN107455343A CN201710808951.5A CN201710808951A CN107455343A CN 107455343 A CN107455343 A CN 107455343A CN 201710808951 A CN201710808951 A CN 201710808951A CN 107455343 A CN107455343 A CN 107455343A
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- CN
- China
- Prior art keywords
- belly
- ovum
- predatory mites
- female
- mite
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000238876 Acari Species 0.000 title claims abstract description 60
- 210000004681 ovum Anatomy 0.000 title claims abstract description 60
- 108010000912 Egg Proteins Proteins 0.000 title claims abstract description 59
- 102000002322 Egg Proteins Human genes 0.000 title claims abstract description 59
- 210000001015 abdomen Anatomy 0.000 title claims abstract description 59
- 238000000034 method Methods 0.000 title claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 241000238631 Hexapoda Species 0.000 claims description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 230000018044 dehydration Effects 0.000 claims description 3
- 238000006297 dehydration reaction Methods 0.000 claims description 3
- 230000013011 mating Effects 0.000 claims description 3
- 230000009182 swimming Effects 0.000 claims description 3
- 238000001802 infusion Methods 0.000 claims description 2
- 238000011160 research Methods 0.000 abstract description 6
- 239000000463 material Substances 0.000 abstract description 3
- 241000406588 Amblyseius Species 0.000 description 13
- 230000033458 reproduction Effects 0.000 description 5
- 210000000349 chromosome Anatomy 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 108010034791 Heterochromatin Proteins 0.000 description 2
- 241001481699 Phytoseiidae Species 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 210000004458 heterochromatin Anatomy 0.000 description 2
- 230000020509 sex determination Effects 0.000 description 2
- 241001014624 Anodontia ovum Species 0.000 description 1
- 108010077544 Chromatin Proteins 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 210000003483 chromatin Anatomy 0.000 description 1
- 230000008144 egg development Effects 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 210000002308 embryonic cell Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000008775 paternal effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Cut open the belly the present invention relates to Predatory Mites a kind of acquisition methods of ovum, it is characterized in that artificially female into the method that not yet output ovum is obtained in mite body from Predatory Mites using the form cut open the belly, comprising the female acquisition into mite of Predatory Mites to be cut open the belly and its placement on slide, Predatory Mites cut open the belly ovum acquisition and its deposit 4 steps.The present invention very can cleverly obtain Predatory Mites and cut open the belly ovum, and the successful acquisition for ovum of cutting open the belly can provide more basic materials for further research Predatory Mites reproduction mechanism, understand fully that the reproduction mechanism of Predatory Mites is advantageous to human-induced change Predatory Mites sex ration again.
Description
Technical field
Cut open the belly the present invention relates to Predatory Mites the acquisition methods of ovum, belong to Predatory Mites fundamental biological knowledge research field.
Background technology
Predatory Mites are important small-sized pest natural enemies, and some Predatory Mites reproductions have particularity, i.e. male and female offspring is equal
Come from development of fertilized ova, but male offspring develops into class monoploid because being disappeared after paternal gene heterochromatinization.This is recognized
To be determined by some Predatory Mites such as special mode of reproduction of Phytoseiidae Predatory Mites, i.e., false arrhenotocia
(Pseudoarrhenotoky) (Hoy, 1985; Nagalkerke & Sabelis 1998; Sabelis et al.
2002).In recent years, Predatory Mites are in addition to as biological control natural enemy, and the research of its biological habit also attracts attention, especially
This special reproduction phenomenon of false arrhenotocia.
Nelson-Rees et al.（1980）Observe west it is quiet walk mite egg development during caryogram change think：
After ovum output in 0-24 hours, the embryonic cell half chromatin of part ovum（3/6）Beginning pyknosis is heterochromatin；After output
In 24-48 hours, there is heterochromatinization phenomenon in most cells in this partial embryonic, and subsequent heterochromatin is discharged cell,
Embryonic development is monoploid male.This result of study be still at present delivered it is most detailed to Phytoseiidae Sex Determination theory
Description, it thinks that initial stage after ovum output occurs for phytoseiid mite Sex Determination.
It is true whether such, in order to explore whether the ovum of Predatory Mites has had monoploid caryogram, i.e. ovum before output
Whether development has occurred and that heterogeneousization or loss for the hemichromosome in the ovum of male mite before output.Need to ovum chromosome of cutting open the belly
Studied, observation chromosome is monoploid or diploid.
But because Predatory Mites individual is small, only hundreds of microns, and be easy in the more fragile operating process of body
Producing damage to it causes body fluid to outflow, and body trunk is flat can not to carry out any experiment, and progress micromanipulation difficulty is quite big, is difficult to
Complete tissue samples are obtained from single individual, therefore either from morphosis to cellular level, research is got up all very tired
It is difficult.Ovum of cutting open the belly how is not obtained in available data, how to take out complete ovum of cutting open the belly, the methods of how ovum preserves after taking out yet
Report.
To solve this series of problems, the present invention provides a kind of method that acquisition Predatory Mites cut open the belly ovum.
The content of the invention
It is an object of the invention to provide a kind of Predatory Mites that obtain to cut open the belly the method for ovum, can be that Predatory Mites be cut open the belly the acquisition of ovum
Acquisition methods are provided, and the research for ovum chromosome of cutting open the belly provides test material.
The present invention be it is artificially female into the method that not yet output ovum is obtained in mite body from Predatory Mites using the form cut open the belly, comprising
Predatory Mites to be cut open the belly it is female into mite acquisition with its placement on slide, Predatory Mites cut open the belly ovum acquisition and its deposit 4 steps
Suddenly, all operations are carried out under Stereo microscope, and concrete operation method is：
（1）The female acquisition into mite of Predatory Mites to be cut open the belly：From Predatory Mites raising colony in picking Predatory Mites ovum individually raising to
Into mite, a female hero is then placed on the small interior of raising and matched, the male and female Predatory Mites just sprouted wings can be mated quickly, be treated
After the completion of mating, continue to provide sufficient prey into mite to female, 12-15 hours are female to expand to have to obtain after ovum into mite belly and wait to cut open
Abdomen Predatory Mites are female into mite；
（2）The female placement into mite on slide of Predatory Mites to be cut open the belly：Clear water is dripped to slide, to be dissected female of picking
Be placed on into mite in the clear water on slide, and with insect needle and fine, soft fur pen keep it is female into the mite back side upward, clear water infusion volume should not
Excessively, avoid female swimming on water layer into mite, it is ensured that female to remain able to touch slide into mite；
（3）Predatory Mites are cut open the belly the acquisition of ovum：The female Hubei Province body portion into mite of Predatory Mites first is pinned with No. 3 thick insect needles, then it is thin with No. 0
Insect needle ventrad since Predatory Mites head, which is gently extruded to belly, to be ruptured, you can sees that Predatory Mites are cut open the belly ovum, then with No. 0
Fine, soft fur nib portion gently pushes the tissue around ovum aside, and this step must be carried out in clear water, prevents that ovum dehydration is shrivelled；
（4）Predatory Mites are cut open the belly the storage of ovum：Predatory Mites ovum of cutting open the belly is in overlong time in water and is easily broken, it is necessary to quickly with No. 0
Fine, soft fur pen, which is positioned over after ovum of cutting open the belly carefully is chosen from water in 100% alcohol, to be deposited standby, that is, is obtained Predatory Mites and cut open the belly ovum.
The present invention very can cleverly obtain Predatory Mites and cut open the belly ovum, and the successful acquisition for ovum of cutting open the belly can be further research
Predatory Mites reproduction mechanism provides more basic materials, understands fully that the reproduction mechanism of Predatory Mites is advantageous to human-induced and changes Predatory Mites again
Sex ration.
Embodiment
Predatory Mites of the embodiment by taking Amblyseius persidolongispinosus as an example are cut open the belly ovum acquisition methods
Concrete operation method includes following 4 part, and all operations are carried out under Stereo microscope：
（1）The female acquisition into mite of Amblyseius persidolongispinosus to be cut open the belly：Picking plan becomes mildewed blunt from the raising colony of Amblyseius persidolongispinosus
Individually a female hero extremely into mite, is then placed on the small interior of raising and matched the ovum of peaceful mite by raising, the male and female plan length just sprouted wings
Hair amblyseius as predatory mite can be mated quickly, be waited after the completion of mating, and continue to provide sufficient prey into mite to female, 12-15 hours it is female into
Mite belly, which expands, that to obtain Amblyseius persidolongispinosus to be cut open the belly after ovum female into mite；
（2）The female placement into mite on slide of Amblyseius persidolongispinosus to be cut open the belly：Clear water is dripped to slide, and picking is waited to solve
Cut open it is female be placed on into mite in the clear water on slide, and with insect needle and fine, soft fur pen keep it is female into the mite back side upward, clear water instills
Amount should not be excessive, avoids female swimming on water layer into mite, it is ensured that female to remain able to touch slide into mite；
（3）Amblyseius persidolongispinosus is cut open the belly the acquisition of ovum：First the female Hubei Province body portion into mite of Amblyseius persidolongispinosus is pinned with No. 3 thick insect needles
Point, then gently extruded to belly and ruptured with No. 0 thin insect needle ventrad since Amblyseius persidolongispinosus head, you can see plan length
Hair amblyseius as predatory mite is cut open the belly ovum, is then gently pushed the tissue around ovum aside with No. 0 fine, soft fur nib portion, this step must be in clear water
Carry out, prevent that ovum dehydration is shrivelled；
（4）Amblyseius persidolongispinosus is cut open the belly the storage of ovum：Amblyseius persidolongispinosus ovum of cutting open the belly is in overlong time in water and is easily broken, and needs
It is positioned over after quickly ovum of cutting open the belly carefully to be chosen from water with No. 0 fine, soft fur pen in 100% alcohol and deposits standby, that is, is intended
The amblyseius as predatory mite that becomes mildewed is cut open the belly ovum.
Claims (1)
- The acquisition methods of ovum 1. Predatory Mites are cut open the belly, it is characterized in that artificially female into obtaining in mite body from Predatory Mites using the form cut open the belly The not yet method of output ovum, cut open the belly comprising the female acquisition into mite of Predatory Mites to be cut open the belly with its placement on slide, Predatory Mites The acquisition of ovum deposits 4 steps with it, and all operations are carried out under Stereo microscope, and concrete operation method is：（1）The female acquisition into mite of Predatory Mites to be cut open the belly：From Predatory Mites raising colony in picking Predatory Mites ovum individually raising to Into mite, a female hero is then placed on the small interior of raising and matched, the male and female Predatory Mites just sprouted wings can be mated quickly, be treated After the completion of mating, continue to provide sufficient prey into mite to female, 12-15 hours are female to expand to have to obtain after ovum into mite belly and wait to cut open Abdomen Predatory Mites are female into mite；（2）The female placement into mite on slide of Predatory Mites to be cut open the belly：Clear water is dripped to slide, to be dissected female of picking Be placed on into mite in the clear water on slide, and with insect needle and fine, soft fur pen keep it is female into the mite back side upward, clear water infusion volume should not Excessively, avoid female swimming on water layer into mite, it is ensured that female to remain able to touch slide into mite；（3）Predatory Mites are cut open the belly the acquisition of ovum：The female Hubei Province body portion into mite of Predatory Mites first is pinned with No. 3 thick insect needles, then it is thin with No. 0 Insect needle ventrad since Predatory Mites head, which is gently extruded to belly, to be ruptured, you can sees that Predatory Mites are cut open the belly ovum, then with No. 0 Fine, soft fur nib portion gently pushes the tissue around ovum aside, and this step must be carried out in clear water, prevents that ovum dehydration is shrivelled；（4）Predatory Mites are cut open the belly the storage of ovum：Predatory Mites ovum of cutting open the belly is in overlong time in water and is easily broken, it is necessary to quickly with No. 0 Fine, soft fur pen, which is positioned over after ovum of cutting open the belly carefully is chosen from water in 100% alcohol, to be deposited standby, that is, is obtained Predatory Mites and cut open the belly ovum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710808951.5A CN107455343A (en) | 2017-09-09 | 2017-09-09 | Predatory Mites are cut open the belly the acquisition methods of ovum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710808951.5A CN107455343A (en) | 2017-09-09 | 2017-09-09 | Predatory Mites are cut open the belly the acquisition methods of ovum |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107455343A true CN107455343A (en) | 2017-12-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710808951.5A Pending CN107455343A (en) | 2017-09-09 | 2017-09-09 | Predatory Mites are cut open the belly the acquisition methods of ovum |
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| Country | Link |
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| CN (1) | CN107455343A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101822239A (en) * | 2010-05-07 | 2010-09-08 | 华南农业大学 | Device and method for collecting mite eggs |
| CN102550489A (en) * | 2012-01-04 | 2012-07-11 | 河北大学 | Artificial propagation method for Ooencyrtus kuvanae |
-
2017
- 2017-09-09 CN CN201710808951.5A patent/CN107455343A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101822239A (en) * | 2010-05-07 | 2010-09-08 | 华南农业大学 | Device and method for collecting mite eggs |
| CN102550489A (en) * | 2012-01-04 | 2012-07-11 | 河北大学 | Artificial propagation method for Ooencyrtus kuvanae |
Non-Patent Citations (3)
| Title |
|---|
| 李隆术等: "《蜱螨学》", 30 November 1988, 重庆出版社 * |
| 蒋洪丽等: "温度对一种新种新小绥螨生长发育的影响", 《中国植物保护学会成立50周年庆祝大会暨2012年学术年会论文集》 * |
| 陈春生等: "茅舍血厉螨核型及染色体的C带、G带的研究", 《动物学研究》 * |
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Application publication date: 20171212 |