CN111887207A - Application of paralytic function of gelechiid chogramma in artificial cultivation of natural enemy insects - Google Patents
Application of paralytic function of gelechiid chogramma in artificial cultivation of natural enemy insects Download PDFInfo
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- CN111887207A CN111887207A CN202010658924.6A CN202010658924A CN111887207A CN 111887207 A CN111887207 A CN 111887207A CN 202010658924 A CN202010658924 A CN 202010658924A CN 111887207 A CN111887207 A CN 111887207A
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- 241000238631 Hexapoda Species 0.000 title claims abstract description 95
- 230000001769 paralizing effect Effects 0.000 title claims abstract description 24
- 238000009395 breeding Methods 0.000 claims abstract description 24
- 230000001488 breeding effect Effects 0.000 claims abstract description 24
- 206010033799 Paralysis Diseases 0.000 claims abstract description 21
- 238000005286 illumination Methods 0.000 claims abstract description 13
- 241000256856 Vespidae Species 0.000 claims abstract description 9
- 241000255777 Lepidoptera Species 0.000 claims abstract description 4
- 241000209140 Triticum Species 0.000 claims description 22
- 235000021307 Triticum Nutrition 0.000 claims description 22
- 241000256816 Braconidae Species 0.000 claims description 18
- 241000595629 Plodia interpunctella Species 0.000 claims description 18
- 238000012258 culturing Methods 0.000 claims description 18
- 241000255791 Bombyx Species 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 14
- 238000011081 inoculation Methods 0.000 claims description 10
- 241000985295 Habrobracon hebetor Species 0.000 claims description 8
- 241000257303 Hymenoptera Species 0.000 claims description 8
- 241000164724 Dastarcus helophoroides Species 0.000 claims description 6
- 230000002745 absorbent Effects 0.000 claims description 6
- 239000002250 absorbent Substances 0.000 claims description 6
- 230000009471 action Effects 0.000 claims description 6
- 241001300674 Plukenetia volubilis Species 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 241000255967 Helicoverpa zea Species 0.000 claims description 4
- 241000346285 Ostrinia furnacalis Species 0.000 claims description 4
- 241000238706 Pyemotes Species 0.000 claims description 4
- 206010039710 Scleroderma Diseases 0.000 claims description 4
- 238000003860 storage Methods 0.000 claims description 4
- 241000753145 Sitotroga cerealella Species 0.000 claims description 3
- 241001125149 Molossus Species 0.000 claims description 2
- 241001340903 Pyralis farinalis Species 0.000 claims description 2
- 241001471302 Tachysurus Species 0.000 claims 1
- 241000256011 Sphingidae Species 0.000 abstract description 22
- 230000008901 benefit Effects 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 241000255896 Galleria mellonella Species 0.000 description 5
- 230000003071 parasitic effect Effects 0.000 description 5
- 241000238876 Acari Species 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 208000002193 Pain Diseases 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 235000013601 eggs Nutrition 0.000 description 3
- 229920000742 Cotton Polymers 0.000 description 2
- 241000353522 Earias insulana Species 0.000 description 2
- 241000256259 Noctuidae Species 0.000 description 2
- 241000563489 Sesamia inferens Species 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000024241 parasitism Effects 0.000 description 2
- 230000001902 propagating effect Effects 0.000 description 2
- 241000142468 Bracon Species 0.000 description 1
- 241000254173 Coleoptera Species 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 241000344244 Rhynchophorus Species 0.000 description 1
- 241001257967 Sesia apiformis Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
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- 230000008569 process Effects 0.000 description 1
- 230000019617 pupation Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Catching Or Destruction (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses an application of utilizing the paralytic function of a gelechiid moth in artificial cultivation of natural enemy insects, which comprises the steps of firstly collecting adults of the gelechiid moth, feeding the adults with artificial feed, collecting old larvae, collecting adults of the gelechiid moth, inoculating the adults of the gelechiid moth into a covered wooden chaff cup filled with the old larvae of the gelechiid moth, putting the cups into a constant-temperature constant-humidity illumination incubator for cultivation, and selecting to obtain adult female gelechiid moth; then artificially breeding or purchasing or collecting lepidoptera insects in the field indoors, breeding to obtain larvae acting on the insects, putting female hornworm molocoris adults into a larva box acting on the insects, and paralyzing the larvae acting on the insects; the larvae of the paralyzed insect are used to inoculate natural enemy insects for culture. The invention plays an important role in the production process of natural enemy insects by utilizing the paralytic function of the hornet mollissima, has low cost and obvious economic benefit, and is suitable for wide application.
Description
Technical Field
The present invention relates to the field of insect feeding; in particular to an application of utilizing the paralytic function of the gelechiid braconid wasps in artificial cultivation of natural enemy insects.
Background
The bracon hebetor say is a parasitic wasp in vitro, belonging to hymenoptera, braconidae, also called braconidae, and widely distributed in China and all over the world. The host range is wide, and more than 40 insects in the lepidoptera noctuidae and the stem borer moth families in fields and warehouses are main parasitic bees of cotton bollworms and corn borers in China and Asian countries.
The tail of the hornet mollissima has stinging, can pierce into a host to release bee venom to paralyze the host, and has strong paralysis capability. The host was paralyzed and no longer active, but it remained alive. Therefore, the habit of the hornet mollissima can be utilized to paralyze potential alternative host insects, so that the activity of the alternative host is weakened, and the nutritional ingredients of the body of the alternative host are not influenced to change, thereby being beneficial to the parasitism and the development of natural enemy insects.
In the process of artificially feeding pyemotes zhonghuajia, hornet moth, branchlet and two-color stinging foot cocoons, if intermediate hosts (such as wheat moths, Indian valley moth, rice moths, mediterranean pink borers, greater wax moths, Asian corn borers and cotton bollworms) are excessively active, the hanging mites and parasitism are influenced, and if the intermediate hosts are frozen, the intermediate hosts are dead when the intermediate hosts are used, the nutritional ingredients of the bodies of the intermediate hosts are changed, and the feeding and the development of natural enemy insects are not facilitated. In addition, part of the intermediate hosts grow faster, the larva stage at the end of the instar is shorter, and pupation is fast, but the hornworm can stay at the larva stage at the end of the instar all the time after being paralyzed by the hornworm, so that enough parasitic time is provided for natural enemy insects.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides application of the paralytic function of the gelechiid molossus in artificial cultivation of natural enemy insects.
In order to achieve the purpose, the invention designs an application of utilizing the paralytic function of the gelechiid braconid wasps in artificial cultivation of natural enemy insects.
The invention also provides a method for artificially breeding natural enemy insects by utilizing the paralytic function of the gelechiid braconid wasps, which comprises the following steps:
(1) preparing a host: collecting adult Plukenetia volubilis, feeding with artificial feed, and collecting old larva;
(2) preparing breeding bees: collecting adult wheat moth bombyx batrachyces, inoculating the adult wheat moth bombyx batrachyces into a covered wooden bran cup filled with old larvae of Indian meal moth, culturing in a constant-temperature constant-humidity illumination incubator, and selecting to obtain adult female wheat moth bombyx batrachyces which is newly emerged on the same day;
(3) preparation of the affected insects: artificially breeding or purchasing in room or collecting lepidoptera insects in field, breeding to obtain larvae of the insects,
(4) inoculating adult female gelechiid smith into an action object: collecting adult female gelechiid molossoma cochleariae newly emerged on the same day in the step (2), putting the adult female gelechiid molossoma cochleariae in the larva box of the acting insects collected in the step (3), and putting the larva box in a constant-temperature constant-humidity illumination incubator for culturing and paralyzing the larva of the acting insects; collecting the larvae of the paralyzed insects, putting the collected larvae into a box filled with absorbent paper, and putting the box into a refrigerator at 4-8 ℃ for cold storage for later use;
(5) culturing the natural enemy insects corresponding to the acting insects: and taking the paralyzed larvae of the affected insects out of a refrigerator, and placing the larvae to room temperature for inoculating the natural enemy insects corresponding to the affected insects for culture.
Further, in the step (2), the inoculation ratio of the female adult hornworm mollissima to the Indian meal moth larvae is 1: 8-12, and at least one male adult hornworm mollissima is required in the adult hornworm mollissima.
Further, in the step (2), the conditions of the constant temperature and humidity illumination incubator are as follows: the temperature was 25 ℃, photoperiod L: D: 16:8, humidity 75%.
Still further, in the step (3), the lepidopteran insects are selected from the group consisting of a wheat moth, a indian meal moth, a rice moth, a mediterranean meal moth, a wax moth, an Asian corn borer and a cotton bollworm;
further, in the step (4), the inoculation ratio of the newly emerged female gelechiid molosson bombycis adults to the larvae of the affected insects on the same day is 1:3 to 5.
Still further, in the step (4), the temperature of the incubator is 25 ℃, the photoperiod is L: D-16: 8, and the humidity is 75%; the culture time is 24 h.
Still further, in the step (5), the natural enemy insects are selected from dastarcus helophoroides first hatched larvae, pyemotes sinensis, rhynchophorus schlegelii, hornet beetle-eaten braconid wasps, bantou pellegelia and two-color stinging calluses.
Still further, in the step (5), the larvae of the paralytic insect-acting insects are inoculated with the natural enemy insects at a ratio of 1:1 to 30.
The invention has the beneficial effects that:
(1) the wheat moth bracon carotovora is easy to obtain. The hornworm is a storage pest parasitic wasp, and can be used for propagating a large number of hornworm larvae or Indian meal moth larvae as intermediate hosts indoors, each hornworm or Indian meal moth can propagate 15-20 hornworm hornworms, wherein the effect of propagating the hornworm by the Indian meal moth is better than that of the hornworm.
(2) The invention has wider host range of the hornworm mollissima, and can parasitize more than 40 insects in lepidoptera noctuidae and stem borer families in fields and warehouses.
(3) According to the invention, a new batch of action objects can be replaced by the gelechiid braconid wasps every 24 hours, the insects can be paralyzed repeatedly, and the gelechiid braconid wasps cannot lay eggs within 24 hours, and the paralytic capacity cannot be reduced until the life of the gelechiid braconid wasps is finished.
(4) After the insect is paralyzed, the nutrient components of the insect are not changed, thereby preventing the insect from pupating prematurely or hardening epidermis and being beneficial to preservation. After paralysis, the mixture can be stored in a refrigerator at 4 ℃ for 7-10 days.
(5) After the insects are paralyzed, the activity of the insects is reduced, and the insects which are natural enemy are beneficial to search and parasitize;
in conclusion, the invention utilizes the paralytic function of the gelechiid braconid wasps to play an important role in the production process of natural enemy insects, has low cost and obvious economic benefit, and is suitable for wide application.
Detailed Description
The present invention is described in further detail below with reference to specific examples so as to be understood by those skilled in the art.
Example 1
The method for artificially cultivating the dastarcus helophoroides by utilizing the paralytic function of the hornet mollissima comprises the following steps of:
(1) preparing a host: collecting adult Plukenetia volubilis, feeding with artificial feed, and collecting old larva;
(2) preparing breeding bees: collecting adult wheat moth bombyx batryticatus, inoculating the adult wheat moth bombyx batryticatus into a covered wooden bran cup filled with old larvae of Indian meal moth, culturing in a constant-temperature and constant-humidity illumination incubator with the temperature of 25 ℃, the photoperiod of L: D: 16:8 and the humidity of 75%, and selecting to obtain adult female wheat moth bombyx batryticatus newly emerged on the same day; wherein the inoculation ratio of the female adult hornworm and Indian meal moth larvae is 1:10, and at least one male adult hornworm is required in the adult hornworm;
(3) preparation of the affected insects: buying old age larva of greater wax moth in market, or collecting old age larva of greater wax moth bred in artificial room for standby;
(4) inoculating adult female gelechiid smith into an action object: collecting adult female gelechiid braconid wasps newly emerged on the same day in the step (2), putting the adult female gelechiid braconid wasps into the box for acting insects collected in the step (3) according to the inoculation ratio of 1:3, and putting the box into a constant-temperature and constant-humidity illumination incubator with the temperature of 25 ℃, the photoperiod of L: D of 16:8 and the humidity of 75% for culturing for 24 hours to paralyze larvae of the acting insects; collecting larvae of paralyzed insects, putting the larvae into a box filled with absorbent paper, and refrigerating the larvae in a refrigerator at 4 ℃ for later use, wherein the paralyzed insects have a paralysis rate of 75%;
(5) preparing natural enemy insects: purchasing or collecting indoor artificially-bred dastarcus helophoroides egg blocks, incubating the egg blocks under a dark condition at the temperature of 25 ℃, and collecting the primarily-incubated larvae for later use;
(6) culturing the natural enemy insects corresponding to the acting insects: taking 20 paralyzed larvae of the greater wax moth, putting the larvae into a square box with a cover and a bottom side length of 6.3cm and a top side length of 7cm, paving a layer of absorbent paper at the bottom of the box, and picking the larvae of the dastarcus helophoroides which are initially hatched by a fine soft hair brush according to the ratio of the greater wax moth: inoculating the dastarcus helophoroides at the ratio of 1:2, covering the cover, and pricking the small hole on the cover with a needle to ventilate; culturing in dark light incubator with 25 deg.C and 70% humidity to obtain a survival rate of 77.5%.
Example 2:
the method for artificially breeding female mites by utilizing the paralytic function of the gelechiid braconid wasps comprises the following steps:
(1) preparing a host: collecting adult Plukenetia volubilis, feeding with artificial feed, and collecting old larva;
(2) preparing breeding bees: collecting adult wheat moth bombyx batryticatus, inoculating the adult wheat moth bombyx batryticatus into a covered wooden bran cup filled with old larvae of Indian meal moth, culturing in a constant-temperature and constant-humidity illumination incubator with the temperature of 25 ℃, the photoperiod of L: D: 16:8 and the humidity of 75%, and selecting to obtain adult female wheat moth bombyx batryticatus newly emerged on the same day; wherein the inoculation ratio of the female adult hornworm and Indian meal moth larvae is 1:8, and at least one male adult hornworm is required in the adult hornworm;
(3) preparation of the affected insects: buying Indian meal moth old larva in market, or collecting Indian meal moth old larva bred in artificial room for standby;
(4) inoculating adult female gelechiid smith into an action object: collecting adult female gelechiid braconid wasps newly emerged on the same day in the step (2), putting the adult female gelechiid braconid wasps into the box for acting insects collected in the step (3) according to the inoculation ratio of 1:5, and putting the box into a constant-temperature and constant-humidity illumination incubator with the temperature of 25 ℃, the photoperiod of L: D of 16:8 and the humidity of 75% for culturing for 24 hours to paralyze larvae of the acting insects; collecting the paralyzed larvae of the insects acting on the insects, putting the collected larvae into a box filled with absorbent paper, and refrigerating the larvae in a refrigerator at 4 ℃ for later use, wherein the paralyzed rate is 98%;
(5) preparing natural enemy insects: purchasing or collecting indoor artificially-fed Chinese beetle and pyemotes tetranychii with mites;
(6) culturing the natural enemy insects corresponding to the acting insects: putting 1 head of paralyzed Indian meal moth larvae into a 2ml uncovered centrifugal tube, then putting 30 head female mites into the tube, and plugging the tube opening of the centrifugal tube by using a cotton wool ball; culturing in dark light incubator at 25 deg.C and humidity of 70% to obtain a mite hanging rate of 100%.
Example 3
The method for artificially culturing the scleroderma Chuanjiangtoufeng by utilizing the paralytic function of the gelechiid hornet comprises the following steps:
(1) preparing a host: collecting adult Plukenetia volubilis, feeding with artificial feed, and collecting old larva;
(2) preparing breeding bees: collecting adult wheat moth bombyx batryticatus, inoculating the adult wheat moth bombyx batryticatus into a covered wooden bran cup filled with old larvae of Indian meal moth, culturing in a constant-temperature and constant-humidity illumination incubator with the temperature of 25 ℃, the photoperiod of L: D: 16:8 and the humidity of 75%, and selecting to obtain adult female wheat moth bombyx batryticatus newly emerged on the same day; wherein the inoculation ratio of the female adult hornworm and Indian meal moth larvae is 1: 8-12, and at least one male adult hornworm is required in the adult hornworm;
(3) preparation of the affected insects: artificially breeding or purchasing or collecting the mediterranean pink borers in the field indoors, and breeding to obtain larvae acting on insects;
(4) inoculating adult female gelechiid smith into an action object: collecting adult female gelechiid braconid wasps newly emerged on the same day in the step (2), putting the adult female gelechiid braconid wasps into the box for acting insects collected in the step (3), and putting the box into a constant-temperature and constant-humidity illumination incubator with the temperature of 25 ℃, the photoperiod L: D: 16:8 and the humidity of 75% to culture for 24 hours to paralyze larvae of the acting insects; collecting the larvae of the paralyzed insects, putting the collected larvae into a box filled with absorbent paper, and putting the box into a refrigerator at 4-8 ℃ for cold storage for later use;
(5) preparing natural enemy insects: purchasing or collecting indoor artificially bred Szechwan scleroderma;
(6) culturing the natural enemy insects corresponding to the acting insects: taking the larvae of the paralyzed insects out of a refrigerator, placing the larvae to room temperature, taking 1 head of the larvae into a 2ml uncovered centrifugal tube, placing 2-3 heads of Szechwan scleroderma drumstick bees into each centrifugal tube, and plugging the tube orifice of the centrifugal tube by using a cotton wool ball; culturing in dark light incubator at 25 deg.C and 70% humidity; the parasitic ratio was 75%.
Other parts not described in detail are prior art. Although the present invention has been described in detail with reference to the above embodiments, it is only a part of the embodiments of the present invention, not all of the embodiments, and other embodiments can be obtained without inventive step according to the embodiments, and the embodiments are within the scope of the present invention.
Claims (9)
1. An application of the paralytic function of the gelechiid molossus in artificial breeding of natural enemy insects.
2. A method for artificially breeding natural enemy insects by utilizing the paralytic function of the gelechiid braconid bees is characterized by comprising the following steps: the method comprises the following steps:
(1) preparing a host: collecting adult Plukenetia volubilis, feeding with artificial feed, and collecting old larva;
(2) preparing breeding bees: collecting adult wheat moth bombyx batrachyces, inoculating the adult wheat moth bombyx batrachyces into a covered wooden bran cup filled with old larvae of Indian meal moth, culturing in a constant-temperature constant-humidity illumination incubator, and selecting to obtain adult female wheat moth bombyx batrachyces which is newly emerged on the same day;
(3) preparation of the affected insects: artificially breeding or purchasing or collecting lepidoptera insects in a field indoors, and breeding to obtain larvae acting on the insects;
(4) inoculating adult female gelechiid smith into an action object: collecting adult female gelechiid molossoma cochleariae newly emerged on the same day in the step (2), putting the adult female gelechiid molossoma cochleariae in the larva box of the acting insects collected in the step (3), and putting the larva box in a constant-temperature constant-humidity illumination incubator for culturing and paralyzing the larva of the acting insects; collecting the larvae of the paralyzed insects, putting the collected larvae into a box filled with absorbent paper, and putting the box into a refrigerator at 4-8 ℃ for cold storage for later use;
(5) culturing the natural enemy insects corresponding to the acting insects: and taking the paralyzed larvae of the affected insects out of a refrigerator, and placing the larvae to room temperature for inoculating the natural enemy insects corresponding to the affected insects for culture.
3. The method for artificially breeding natural enemy insects by utilizing the paralytic function of the gelechiid bracon hebetor according to claim 2, wherein the natural enemy insects comprise: in the step (2), the inoculation ratio of the female adult wheat moth bracon hebetor to the Indian meal moth larvae is 1: 8-12, and at least one male adult wheat moth bracon hebetor is required to be inoculated in the adult wheat moth bracon hebetor.
4. The method for artificially breeding natural enemy insects by using the paralytic function of the hornet bombycis according to claim 2 or 3, wherein: in the step (2), the conditions of the constant temperature and humidity illumination incubator are as follows: the temperature was 25 ℃, photoperiod L: D: 16:8, humidity 75%.
5. The method for artificially breeding natural enemy insects by utilizing the paralytic function of the gelechiid bracon hebetor according to claim 2, wherein the natural enemy insects comprise: in the step (3), the lepidoptera insects are selected from the group consisting of a wheat moth, a indian meal moth, a rice moth, a mediterranean meal moth, a wax moth, an Asian corn borer and a cotton bollworm.
6. The method for artificially breeding natural enemy insects by utilizing the paralytic function of the gelechiid bracon hebetor according to claim 2, wherein the natural enemy insects comprise: in the step (4), the inoculation ratio of the adult female gelechiid molosson coides newly emerged on the same day to the larva acting on the insects is 1:3 to 5.
7. The method for artificially breeding natural enemy insects by using the paralytic function of the hornet bombycis according to claim 2 or 6, wherein: in the step (4), the temperature of the incubator is 25 ℃, the photoperiod is L: D: 16:8, and the humidity is 75%; the culture time is 24 h.
8. The method for artificially breeding natural enemy insects by utilizing the paralytic function of the gelechiid bracon hebetor according to claim 2, wherein the natural enemy insects comprise: in the step (5), the natural enemy insects are selected from dastarcus helophoroides first hatched larvae, pyemotes sinensis, swollen leg peak of Sichuan scleroderma, branchionurus flavipes, pelteobagrus paradoxa and two-color spinulodermus.
9. The method for artificially breeding natural enemy insects by using the paralytic function of the hornet bombycis according to claim 2 or 8, wherein: in the step (5), the inoculation ratio of the larvae of the paralytic insects to the natural enemy insects is 1:1 to 3.
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CN113287574A (en) * | 2021-04-30 | 2021-08-24 | 中国热带农业科学院环境与植物保护研究所 | Method for propagating blue Changdou gold wasps |
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CN115885932A (en) * | 2022-11-18 | 2023-04-04 | 中国热带农业科学院环境与植物保护研究所 | Method for directionally breeding high-temperature resistant corpus luteum strain of gelechiid smith |
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