CN105557641A - Method and device for collecting and conserving habrobracon hebetor - Google Patents
Method and device for collecting and conserving habrobracon hebetor Download PDFInfo
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- CN105557641A CN105557641A CN201510978715.9A CN201510978715A CN105557641A CN 105557641 A CN105557641 A CN 105557641A CN 201510978715 A CN201510978715 A CN 201510978715A CN 105557641 A CN105557641 A CN 105557641A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention discloses a device for collecting and conserving habrobracon hebetor. The device comprises a collection cabin, a collection bottle, a breeding bottle and a bracket, wherein the collection cabin is an openable cylindrical mechanism; a horn-shaped collection cover is arranged at the bottom of the collection cabin and lined with a layer of gauze screen; a light source and a pull ring are arranged at the upper part of the collection cabin; the collection bottle is embedded in the collection cabin; a bottom cover is arranged at the bottom of the collection bottle; during collection, the bottom cover is taken down; a bottle opening is formed in the top of the collection bottle and corresponds to the light source in the collection cabin in position; and light of the light source passes through the collection bottle in the collection cabin and then enters an external environment through the collection cover. According to the device, habrobracon hebetor breeds are collected by utilizing the positive phototropism of the habrobracon hebetor, so that the manpower input in collection work is reduced; the habrobracon hebetor is guided to enter and leave from the breeding bottle by controlling the light source, so that the interference of external force on hosts and parasitic wasp is reduced and the success rate of breeding is increased; and collection and breeding devices are designed in a unified way, so that the degree of matching between the devices is increased, the escape opportunities of the habrobracon hebetor can be effectively reduced, and the standardized process of biocontrol product research and development of the habrobracon hebetor can be promoted.
Description
Technical field
The invention provides a kind of collection and the method for the soft cocoon honeybee of child care gelechiid and device thereof, belong to biological control device technique field.
Background technology
The soft cocoon honeybee of gelechiid (
habrobraconhebetor) be a kind of distribution and host range epizoite honeybee all widely, can parasitic multiple lepidopteran insect larvae.Under natural conditions, soft cocoon honeybee occurs in storage environment along with storage pest gelechiid usually, collects and child care it, contributes to research and utilizes this kind of natural enemy insect resource.The collection of the soft cocoon honeybee of current gelechiid often uses the instruments such as test tube manually sporadicly to catch, and is then pooled to access lepidopteran insect larvae in larger container and carries out child care.Honeybee enlivens and has very strong photopositive gelechiid soft cocoon, because each vessel port differs, becomes honeybee often to escape when it being shifted between each container, wayward; This larva of bee is epizoite, easily making container move or vibrate, then making this larva of bee be subject to external force and depart from host when repeatedly catching because becoming honeybee to escape, and causes to grow to stop or death, have impact on child care effect.In the technical research of other near field, the existing a small amount of report of the collection technique for aphid parasite.Its collection mode is broadly divided into two classes: a class uses external force mainly to adopt the mode of bleeding to collect, and receives catch device, a kind of cocoon honeybee counting collection equipment, a kind of easy device etc. becoming honeybee for collecting aphid parasite as portable cocoon honeybee; Another kind of is collect by the photopositive of aphid parasite, as aphid parasite becomes honeybee collector, automatically can collect the receipts honeybee bag, the special trapper of aphidius gifuensis etc. of aphid parasite, under the host of aphid parasite often appears at greenhouse, such technology mainly for the Environment Design of the natural daylight abundances such as tobacco planting booth, and does not comprise the supporting technology link be connected mutually with follow-up child care work in relevant report usually yet.
Summary of the invention
The invention provides a kind of collection and the method for the soft cocoon honeybee of child care gelechiid and device, realize a kind of can collection and nursing method by the soft cocoon honeybee of the gelechiid of supporting enforcement, reduce the artificial input in collection process, reduce and the escape avoiding the soft cocoon honeybee of gelechiid to become honeybee, reduce the interference to host and parasitoid larva in child care process.
One of the present invention is collected and child care gelechiid soft cocoon honeybee device, it is characterized in that: comprise collecting bin, receiving flask, breed bottle and support, wherein, collecting bin is openable tubular mechanism, the bottom of collecting bin is provided with the collecting hood of bell mouth shape, is lined with warp layer net in collecting hood; The aperture of above-mentioned gauze is that 2 ~ 3mm(can allow the soft cocoon honeybee of gelechiid to pass through, and the storage pest intercepting the build such as Indian meal moth, rice moth larger enters); The top of collecting bin is provided with light source and draw ring; Receiving flask is embedded in collecting bin; The bottom of receiving flask is provided with transparent bottom, is taken off by bottom during collection; Receiving flask top is provided with opening, and corresponding with the light source position in collecting bin, opening is furnished with bottle cap, is loaded onto by bottle cap during collection; The light of light source enters external environment by collecting hood again after the receiving flask in collecting bin.
Described bottle of breeding is made up of body, bottleneck and bottle cap, and body and lid are that the grey material of low light transmission is made, bottleneck be transparent material make circular; The periphery of bottleneck offers circular hole and ventilation hole, and ventilation hole inwall is coated with gauze, and the soft cocoon honeybee of gelechiid can be stoped to escape; The periphery of bottle cap is provided with two symmetrical through holes, and corresponding with the circular hole of bottleneck or ventilation hole position, bore size is identical with ventilation hole with the circular hole of bottleneck.In body bottom, corresponding circular hole and ventilation hole position have solid and hollow arrow to be marked respectively.
When receiving flask puts into collecting bin, opening is put towards light source direction, and light source is built with white led lamps, and after light source is opened, the outer wall of light source, collecting bin, collecting hood is light tight; The sidewall of receiving flask is light tight, printing opacity at the bottom of opening and shoulder portion and bottle; During collection, hung at higher position by the draw ring at receiving flask top, light source irradiates from top to bottom; Also can choice for use with the support of stationary fixture, light source levels is irradiated.
good effect of the present invention is:the present invention utilizes the photopositive of the soft cocoon honeybee of gelechiid to carry out the collection of honeybee kind, simplifies the human input in collection work; Guiding gelechiid soft cocoon honeybee to breed bottle from being advanced into and leaving by controlling light source azimuth, reducing the interference of external force to host and parasitic wasp, contributing to raising and breed success rate; Collected and breeding device by Uniting, matching degree between device is improved, effectively can reduce the meeting of gelechiid soft cocoon honeybee escape machine, also contribute to the standardized process advancing gelechiid soft cocoon honeybee biological and ecological methods to prevent plant disease, pests, and erosion research and development of products.
Accompanying drawing explanation
Fig. 1 is gathering unit structural representation of the present invention;
Fig. 2 is supporting structure schematic diagram of the present invention;
Fig. 3 is that the present invention breeds a bottle body structural representation;
Fig. 4 is that the present invention breeds bottle cap structural representation;
In figure, 1, collecting bin; 2, receiving flask; 3, opening; 4, door; 5, light source; 6, collecting hood; 7, gauze; 8, draw ring; 9, stationary fixture; 10 supports; 11, body; 12, bottleneck; 13, bottle cap; 14, circular hole; 15, ventilation hole; 16, through hole; 17, through hole.
Embodiment
To be illustrated further description the present invention by following examples, and do not limit the present invention in any way, under the prerequisite not deviating from technical solution of the present invention, any change that those of ordinary skill in the art made for the present invention easily realize or change all will fall within right of the present invention.
embodiment 1
According to Fig. 1 ~ Fig. 2, apparatus of the present invention comprise collecting bin 1, receiving flask 2, breed bottle and support 10, and wherein, collecting bin 1 is openable tubular mechanism, and the bottom of collecting bin 1 is provided with the collecting hood 6 of bell mouth shape, is lined with warp layer net 7 in collecting hood 6; The aperture of above-mentioned gauze 7 is that 2 ~ 3mm(can allow the soft cocoon honeybee of gelechiid to pass through, and the storage pest intercepting the build such as Indian meal moth, rice moth larger enters); The top of collecting bin 1 is provided with light source 5 and draw ring 8; Receiving flask 2 is embedded in collecting bin 1; The bottom of receiving flask 2 is provided with bottom, is taken off by bottom during collection; Receiving flask 2 top is provided with opening 3, and corresponding with light source 5 position in collecting bin 1, opening 3 is furnished with bottle cap, is loaded onto by bottle cap during collection; The light of light source 5 enters external environment by collecting hood 6 again after the receiving flask 2 in collecting bin 1.The outer wall of light source 5, collecting bin 1, collecting hood 6 is light tight; The sidewall of receiving flask 2 is light tight, printing opacity at the bottom of bottleneck 3 and bottle.During collection, can the draw ring 8 at choice for use tubular structure top hang at higher position, light irradiates from top to bottom; Also can choice for use with the support of stationary fixture 9, light source levels is irradiated.Gelechiid soft cocoon honeybee, because of the strong photopositive of himself, can enter collecting hood 6 by gauze 7 voluntarily and collect in receiving flask 2.Open collecting bin 1 after waiting for a period of time, take out the receiving flask 2 of having collected gelechiid soft cocoon honeybee and the collection of the soft cocoon honeybee of gelechiid at the bottom of loading onto bottle, can be completed; Then also can install new receiving flask 2 to continue to collect.
Breed bottle shown in Fig. 3, Fig. 4 of the present invention, be made up of body 11, bottleneck 12 and bottle cap 13, wherein, body 11 uses grey material to reduce light transmittance, and bottleneck 12 is transparent ring, and lid 13 uses the grey material identical with body 11 to reduce light transmittance; Have 2 circular holes 14 that are adjacent, same size and ventilation hole 15 at bottleneck 12 sustained height place, circular hole size and receiving flask open top 3 match, and can just put into; Circular hole 14 is unobstructed, and ventilation hole 15 inwall is coated with gauze as ventilation hole, and this gauze aperture can stop the soft cocoon honeybee of gelechiid to escape; There is solid and hollow arrow to be marked respectively in the position of the corresponding circular hole 14 of body 11 and ventilation hole 15; Bottle cap 13 has the through hole 16 and 17 that 2 positions are relative, its size is identical with ventilation hole 15 circular hole with the circular hole 14 of bottleneck 12, and after bottle cap 13 covers body 11, one of them through hole 16 or through hole 17 can overlap just with the circular hole 14 of bottleneck 12 or ventilation hole 15.
Before breeding, open bottle cap 13, in body 11, put into 3-10 head Indian meal moth or rice moth 3 above larva in age, can select to put into and be beneficial to host larva through repeatedly folding paper or other abrasive articles and creep and increase inner surface of container and amass; Then cover bottle cap 13, make the through hole 16 of bottle cap 13 overlapping with the circular hole 14 of body 11; By bottom receiving flask 2 towards light, combine in wait gathering in swarms bottom time, open top 3 bottle cap is opened, insert this overlapping circular hole, block light at the bottom of receiving flask 2 bottles, because bottleneck 12 is transparent, the soft cocoon honeybee of gelechiid in receiving flask 2 by front circular hole 17 through light attract, can certainly be advanced into breeding device inside; After entering appropriate honeybee, remove the shade at the bottom of receiving flask 2 bottles, receiving flask 2 is extracted, cover receiving flask 2 open top 3 bottle cap, rotate and breed the bottle cap 13 of bottle, the circular hole 16 of bottle cap 13 is overlapped with the ventilation hole 15 of bottleneck 12, standingly treat gelechiid soft cocoon honeybee host larva in parasitic bottle voluntarily.After 24h, rotary bottle cover 13 makes circular hole 14 overlapping with through hole 16, block through hole 17 place light, the soft cocoon honeybee of gelechiid attracts to leave voluntarily by through hole 16 place light, and receiving flask 2 or transparent vessel now also can be used to reclaim the unified process of the soft cocoon honeybee of gelechiid at this overlapping circular hole place.After the soft cocoon honeybee of parental generation gelechiid leaves breeding device, rotary bottle cover 13 makes through hole 16 overlap with ventilation hole 15, recovers ventilation state, leaves standstill and treats that filial generation honeybee grows.The bottle cap 13 of breeding bottle can be opened after filial generation honeybee cocoons, filial generation honeybee is separated one by one loading test tube and completes growth, carry out the work such as the qualification of honeybee kind and population purifying.
test example 1:
The feed storehouse collection soft cocoon honeybee of gelechiid and using Indian meal moth larva as host's child care.Selected storehouse after preliminary investigation, adopt support mode to arrange gathering unit, lay 3 points in storehouse side near wall, Cheng Yilie, level, towards storehouse internal radiation, is opened light source at post sunset and is collected, terminate after 1h.Collect insect 17, visually observe and collect the soft cocoon honeybee of gelechiid about 11.Equipment and insect are taken back and breed room, prepare 3 and breed bottle, put in every bottle 3 age above Indian meal moth larva 10, and put into folding after blank sheet of paper so that Indian meal moth larva creeps.Being guided respectively by insect in receiving flask 1,2,3 to enter breeds in bottle 1,2,3, and host-location is parasitic voluntarily to treat it in left at room temperature.Guide parental generation honeybee to leave after 24h and breed bottle, continue to observe filial generation honeybee and grow.After filial generation honeybee cocoons, statistics host benumbs number, parasitic number, filial generation honeybee cocoon sum; Honeybee cocoon turn black after, open and breed bottle filial generation honeybee single head is distributed into test tube, after it is sprouted wings, carry out the qualification of honeybee kind, effective honeybee is merged and sets up child care population.
Gelechiid soft cocoon honeybee quantity statistics collected by table 1 storehouse
| Receiving flask 1 | Receiving flask 2 | Receiving flask 3 | |
| Total borer population | 7 | 5 | 5 |
| Estimate effective borer population | 4 | 3 | 4 |
Table 2 uses Indian meal moth larva to breed the soft cocoon honeybee of gelechiid
| Breed bottle 1 | Breed bottle 2 | Breed bottle 3 | |
| Host benumbs number | 10 | 10 | 10 |
| The parasitic number of host | 7 | 6 | 8 |
| Filial generation honeybee cocoons sum | 22 | 19 | 25 |
| Emergence number | 22 | 18 | 25 |
test example 2:
The peasant household warehouse collection soft cocoon honeybee of gelechiid and using rice moth larva as host's child care.Selected bleeding point after preliminary investigation, hangs 1 cover gathering unit, starting to collect by day, terminating opening light source after window shading after 1h.Collect insect 11, visually observe and collect the soft cocoon honeybee of gelechiid about 8.Equipment and insect are taken back and breed room, prepare 2 and breed bottle, put in every bottle 3 age above rice moth larva 10, and put into folding after blank sheet of paper so that rice moth larva creeps.Being guided respectively by insect in receiving flask to enter breeds in bottle 1,2, and host-location is parasitic voluntarily to treat it in left at room temperature.Guide parental generation honeybee to leave after 24h and breed bottle, continue to observe filial generation honeybee and grow.After filial generation honeybee cocoons, statistics host benumbs number, parasitic number, filial generation honeybee cocoon sum; Honeybee cocoon turn black after, open and breed bottle filial generation honeybee single head is distributed into test tube, after it is sprouted wings, carry out the qualification of honeybee kind, effective honeybee is merged and sets up child care population.
Table 3 uses rice moth larva to breed the soft cocoon honeybee of gelechiid
| Breed bottle 1 | Breed bottle 2 | |
| Host benumbs number | 9 | 8 |
| The parasitic number of host | 8 | 6 |
| Filial generation honeybee cocoons sum | 27 | 23 |
| Emergence number | 25 | 19 |
Claims (1)
1. collect the device with the soft cocoon honeybee of child care gelechiid for one kind, it is characterized in that: comprise collecting bin, receiving flask, breed bottle and support, wherein, collecting bin is openable tubular mechanism, the bottom of collecting bin is provided with the collecting hood of bell mouth shape, is lined with warp layer net in collecting hood; The top of collecting bin is provided with light source and draw ring; Receiving flask is embedded in collecting bin; The bottom of receiving flask is provided with bottom, is taken off by bottom during collection; Receiving flask top is provided with opening, corresponding with the light source position in collecting bin; The light of light source enters external environment by collecting hood again after the receiving flask in collecting bin;
Described bottle of breeding is made up of body, bottleneck and bottle cap, and body and lid are that the grey material of low light transmission is made, bottleneck be transparent material make circular; The periphery of bottleneck offers circular hole and ventilation hole, and ventilation hole inwall is coated with gauze, and the soft cocoon honeybee of gelechiid can be stoped to escape; The periphery of bottle cap is provided with two symmetrical through holes, and corresponding with the circular hole of bottleneck or ventilation hole position, bore size is identical with ventilation hole with the circular hole of bottleneck; In body bottom, corresponding circular hole and ventilation hole position have solid and hollow arrow mark respectively.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510978715.9A CN105557641B (en) | 2015-12-24 | 2015-12-24 | A kind of method and device collected with the soft cocoon honeybee of child care gelechiid |
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| CN201510978715.9A CN105557641B (en) | 2015-12-24 | 2015-12-24 | A kind of method and device collected with the soft cocoon honeybee of child care gelechiid |
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| CN105557641A true CN105557641A (en) | 2016-05-11 |
| CN105557641B CN105557641B (en) | 2018-02-06 |
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| CN106719447A (en) * | 2016-12-02 | 2017-05-31 | 武汉超拓生态农业有限公司 | A kind of flat angle water common people larva material residue easy to clean photoinduction automatic separating apparatus of speck |
| CN107114329A (en) * | 2017-06-02 | 2017-09-01 | 江西农业大学 | A kind of assembly type aphidius gifuensis is into honeybee automatic collecting device and its operating method |
| CN108541675A (en) * | 2018-06-27 | 2018-09-18 | 中国农业科学院蜜蜂研究所 | A kind of harvester of living body bee sample |
| CN110024749A (en) * | 2019-04-11 | 2019-07-19 | 遵义市林业科学研究所 | A kind of tiny golden wasp woodland child care device and nursing method |
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| CN106719447A (en) * | 2016-12-02 | 2017-05-31 | 武汉超拓生态农业有限公司 | A kind of flat angle water common people larva material residue easy to clean photoinduction automatic separating apparatus of speck |
| CN107114329A (en) * | 2017-06-02 | 2017-09-01 | 江西农业大学 | A kind of assembly type aphidius gifuensis is into honeybee automatic collecting device and its operating method |
| CN108541675A (en) * | 2018-06-27 | 2018-09-18 | 中国农业科学院蜜蜂研究所 | A kind of harvester of living body bee sample |
| CN110024749A (en) * | 2019-04-11 | 2019-07-19 | 遵义市林业科学研究所 | A kind of tiny golden wasp woodland child care device and nursing method |
| CN110024749B (en) * | 2019-04-11 | 2021-11-16 | 遵义市林业科学研究所 | Nursing method for small hornets |
| CN110199944A (en) * | 2019-05-30 | 2019-09-06 | 吉林农业大学 | A method of utilizing transparent screw and vision decoy test experiments animal attraction |
| CN110367203A (en) * | 2019-06-06 | 2019-10-25 | 贵州省烟草公司贵阳市公司 | A kind of artificial batch feeding packaging microbracon hebetor living body at bee method |
| CN110367203B (en) * | 2019-06-06 | 2021-08-20 | 贵州省烟草公司贵阳市公司 | Method for artificially breeding packaged live adult wheat moth cocoon bees in batch |
| CN110235857B (en) * | 2019-06-06 | 2021-08-20 | 贵州省烟草公司贵阳市公司 | Method for killing tobacco leaf pink borers in storage by feeding and transporting adult meadowworms with tobacco pink borers as hosts |
| CN110235857A (en) * | 2019-06-06 | 2019-09-17 | 贵州省烟草公司贵阳市公司 | A method of transport microbracon hebetor is raised for host with cacac moth and kills Storage tobacco cacac moth at bee |
| CN111066689A (en) * | 2019-12-11 | 2020-04-28 | 浙江大学 | Device for preventing and controlling nest insects by using parasitic wasps and application thereof |
| CN111771819A (en) * | 2020-07-09 | 2020-10-16 | 武汉市园林科学研究院 | Application of galleria mellonella larva as intermediate host in artificial breeding of dastarcus helophoroides |
| CN111887207A (en) * | 2020-07-09 | 2020-11-06 | 武汉市园林科学研究院 | Application of paralytic function of gelechiid chogramma in artificial cultivation of natural enemy insects |
| CN112471080A (en) * | 2020-12-16 | 2021-03-12 | 湖南省烟草公司长沙市公司 | Device and method for collecting lepidoptera insect larva parasitic wasps in field |
| CN115885932A (en) * | 2022-11-18 | 2023-04-04 | 中国热带农业科学院环境与植物保护研究所 | Method for directionally breeding high-temperature resistant corpus luteum strain of gelechiid smith |
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