CN103004697A

CN103004697A - General flour mite purifying and feeding method and device

Info

Publication number: CN103004697A
Application number: CN2012104203665A
Authority: CN
Inventors: 金道超; 刘婷
Original assignee: Guizhou University
Current assignee: Guizhou University
Priority date: 2012-10-29
Filing date: 2012-10-29
Publication date: 2013-04-03
Anticipated expiration: 2032-10-29
Also published as: CN103004697B

Abstract

The invention discloses a general flour mite purifying and feeding method and a general flour mite purifying and feeding device. The method comprises the following steps of: at a purifying stage, placing a feed into a basic feeding container, and inoculating a female oogenesis mite or a pair of mating mites into the basic feeding container for feeding; after the flour mite(s) in the basic feeding container bear young, communicating the basic feeding container with an extension feeding container filled with a feed through a connecting tube, and entering an extension stage till the flour mites in the basic feeding container enter the extension feeding container automatically through the connecting tube; and after the flour mites finish moving, removing the connecting tube, sealing the interface of the extension feeding container, and taking the flour mites for later use to finish collection of the flour mites. A way for communicating the basic feeding container with the extension feeding container through the connecting tube is adopted, and flour mites actively enter a new environment due to taxis according to a designed route after foods are reduced and the environment is deteriorated, so that the aim of controlling the moving route of flour mites is fulfilled, and damages and losses of mite bodies during manual replacement of feeders and mite body movement are avoided.

Description

General flour mite purifying raising method and device

Technical field

The present invention relates to the biological study field, especially a kind of general flour mite purifying raising method and device.

Background technology

Room and reserve flour mite are the large monoids of one in the flour mite, and their kinds are many, and it is wide to distribute, and is the Major Members in the edifice ecosystem.Its feeding habits are very extensive, can endanger the grain, traditional Chinese medicine, dry fruit, fabric of storage etc., these article qualities are descended, even destroy fully.Room and reserve flour mite can multiply under suitable temperature and humidity conditions in a large number, can people's living environment be polluted, its excreta, secretion, the skin under sloughing off and the lysate of dead body thereof all are very strong allergens, can cause people's multiple allergic disease; Wherein some kind survival ability is very strong, if after entering human body by certain approach, can in the existence of people's endobiosis, cause acariasis in the human body.Therefore, room and reserve flour mite are the insect of harm reserve, are again the pathogene of harm humans health.

Room and reserve flour mite receive people's concern day by day because harm is larger, and its research work also extensively and is rapidly being carried out.In addition, utilize flour mite as feed, produce Predatory Mites and carry out the important channel that biological control also is the development green agriculture.No matter which kind of scientific research, its most basic basis are the acquisitions of experimental subjects or experiment material.Otherwise any research work all is water without a source and a tree without roots.For the small research object of build, experiment material is except a few studies can obtain by the natural world collection (when needing quantity more, purebred being difficult to obtains by gathering to separate), major part still will obtain by raising the material of purifying, thereby guarantees authenticity and the reliability of result of study.Aspect the raising of mite class, forefathers have carried out a lot of explorations, have invented the individual culture apparatus such as the individual feeding device.This kind device has been brought into play important effect really in individual feeding, but just cumbersome when further enlarging cultured space again, need artificially to move the mite body at microscopically, operating difficulties not only, and cause easily the damage of mite body and lose, made originally and reduced with regard to purifying product quantity few in number, and be unfavorable for enlarging and cultivate.

Summary of the invention

The objective of the invention is: a kind of general flour mite purifying raising method and device are provided, and it can not only purifying raises the mite kind, and workload is little, easy damaged and lose the mite body not, and the equipment configuration that adopts is simple, and is easy and simple to handle, to overcome the deficiencies in the prior art.

The present invention is achieved in that general flour mite purifying raising method, in purification phase, puts into feed in basis raising container, and one of the female mite of oogenesis or a pair of just the raising in the container on mating mite access basis are raised; After flour mite in container is raised on the basis produces the offspring, container is raised on the basis to be communicated with by taking over the expansion raising container that is placed with feed, enter extension phase, wait for that the flour mite in the basis raising container is raised (according to expansion quantity and the volume of the definite expansion of required mite amount feeder) in the container by taking over from being advanced into expansion; Flour mite move finish after, remove adapter, seal the interface that container is raised in expansion, take the collection of namely finishing flour mite for subsequent use away.

When the needs conservation, in the raising container that is placed with feed, put into the mite kind that is no more than raising vessel volume 1/3, surpass at breeding amount and raised container contents at long-pending 1/2 o'clock, another is placed with the raising container of feed by taking over connection; Repeat above-mentioned steps and realize conservation.

General flour mite purifying raising device comprises basis raising container, raises container on the basis and is connected by adapter and expansion raising container; Raise container and expansion on the basis and raise container and be respectively equipped with and take over corresponding interface, and raise container on the basis and expansion raising container is equipped with air-vent.

Adapter is that the pyramidal structure of hollow, the end that its diameter is large are connected to the basis and raise on the container, and the end that its diameter is little is connected to expansion and raises on the container.Take over and adopt pyramidal structure that flour mite is entered the expansion raising container from basis raising container easily, and because an end of connection expansion raising container is little, the flour mite that enters extension container is difficult to climb back the expansion feeder of basic feeder or close basic feeder one end.

Raise container in expansion and raise container by taking over another expansion of connection, the larger diameter end of adapter is connected near the expansion of basis raising container and raises on the container.As required can the infinite expanding cultured space.

Owing to adopted technique scheme, compared with prior art, the present invention adopts to take over and is communicated with basis raising container and expands the mode of raising container, utilize the flour mite taxis, make flour mite after food minimizing and environmental deterioration, can initiatively enter in the new environment according to design route, thereby reach the purpose of controlling flour mite behavior route.Because the mite corpse, the ight soil that in the raising stage, produce and slough off under the foreign material such as cot pile up for a long time the generation poisonous and harmful substance, it is healthy to affect the mite body, therefore, prior art needs regularly artificial mobile flour mite usually, clear up its feeding environment, the present invention then can effectively address this problem, and has avoided the injury and the loss that in artificial replacing feeder and the mobile mite body process mite body are caused; And receive mite conveniently, the container of directly taking aequum away gets final product; Because cultured space can infinitely be expanded in the needs scope, therefore suitable long-term conservation is saved the storage area, is convenient to transportation and exchanges, and reduces heavy subculture work, and convenient operation is even the layman also can operate.Reduce the waste of human and material resources resource unnecessary in the production operation; By changing the size of raising container, can expand to some storage insect feeder.The inventive method is simple, is easy to industrialization, and result of use is good.

Description of drawings

Accompanying drawing 1 is structural representation of the present invention;

Accompanying drawing 2 is expansion structure schematic diagram of the present invention.

Embodiment

Embodiments of the invention: the structure of general flour mite purifying raising device comprises basis raising container 1 as shown in Figure 1, raises container 1 on the basis and is connected by adapter 2 and expansion raising container 3; Raise container 1 and expansion on the basis and raise container 3 and be respectively equipped with and take over 2 corresponding interfaces, and raise container 1 and expansion on the basis and raise container 3 and be equipped with air-vent 4; Taking over 2 is the pyramidal structure of hollow, takes over the large end of 2 diameters and is connected on the basis raising container 1, and the end that its diameter is little is connected to expansion and raises on the container 3; Can pass through to take over 2 at expansion raising container 3 as required and connect another expansion raising container 3, the larger diameter end of adapter 2 is connected near the basis and raises on the expansion raising container 3 of container 1; Utilize this mode, cultured space can infinitely be expanded in the needs scope.As shown in Figure 2.

The making of container 1 is raised on the basis: adopt the 0.2ml centrifuge tube, the bottom is roasting soft with alcolhol burner, make the flat tubule that length is reduced to its former length 1/2, prick diameter greater than the hole of 2mm in a side of body, and stick black filter paper with ventilative, namely make the basis and raise container 1, the mouth of pipe of centrifuge tube becomes the interface that container 1 is raised on the basis.

The making of adapter 2: adopt the pipettor gun head (model can be selected in proportion according to the size of expansion feeder the pipettor gun head of different model) of 1ul as taking over 2.

The making of expansion raising container 3: can select according to the volume needs in the 0.5ml(model) bottom of centrifuge tube is got one to the hole of adapter 2, become expansion and raise the interface of container 3, prick diameter greater than the hole of 2mm in a side of body, and stick black filter paper with ventilative, namely make expansion and raise container 3; The expansion of the usefulness that finishes up is at last raised the container 3 same centrifuge tubes that adopt and is made, but its slightly larger in diameter is raised container 3 in expansion before, and gets final product as its interface with the mouth of pipe.

General flour mite purifying raising method:

Step 1, purification phase: in raising container 1, the basis puts into a small amount of feed, and one of the female mite of access oogenesis or a pair ofly just raise the mating mite.For preventing natural death or peerless, can establish a plurality of repetitions; Be put in the control environment;

Step 2, extension phase: by observing, treat that the flour mite that raise in the container 1 on the basis produces the offspring, open the basis and raise the capping of the interface of container 1, will take over large one the terminating on this interface of 2 diameters, take over 2 the other end and insert in the interface that expansion raises container 3; Along with increasing of mite amount in the basis raising container 1, can be by taking over 2 with 3 connections of a plurality of expansions raising containers, as shown in Figure 2; Wait for that the flour mite that raise in the container 1 on the basis climbs into voluntarily expansion and raises in the container 3 by taking over 2, observe the basis and raise container 1 and take over when movable without the mite body in 2 that remove and take over 2, the interface of expansion being raised container 3 seals with black filter paper.

Step 3, collection phase: container 3 is raised in the expansion of choosing suitable output according to required mite amount, and directly takes for subsequent use getting final product away after sealing its interface, and it is for subsequent use also can directly to take off ending feeder 7.

Step 4, conservation stage: the expansion that optional step three is collected is raised container 3 as initial feeder, after the flour mite automatic moving is finished, removes first expansion and raises container 3 and take over 2; After continuing to raise a period of time, when treating that mite density is larger, get the new centrifuge tube that is placed with feed again and make the purpose feeder, former purpose feeder then becomes initial feeder.The repetition above-mentioned steps gets final product.The conservation stage also can be established a plurality of repetitions.

Claims

1. a general flour mite purifying raising method is characterized in that: in purification phase, put into feed in basis raising container, and one of the female mite of oogenesis or a pair of just the raising in the container on mating mite access basis are raised; After flour mite in basis raising container produces the offspring, container is raised on the basis be communicated with by the expansion raising container of taking over and be placed with feed, enter extension phase, treat that the flour mite in the basis raising container is raised in the container by taking over from being advanced into to expand; Flour mite move finish after, remove adapter, seal the interface that container is raised in expansion, take the collection of namely finishing flour mite for subsequent use away.

2. general flour mite purifying raising method according to claim 1, it is characterized in that: when the needs conservation, in the raising container that is placed with feed, put into the mite kind that is no more than raising vessel volume 1/3, surpass long-pending 1/2 o'clock of raising container contents at breeding amount, another is placed with the raising container of feed by taking over connection; Repeat above-mentioned steps and realize conservation.

3. a general flour mite purifying raising device comprises basis raising container (1), it is characterized in that: raise container (1) on the basis and be connected by adapter (2) and expansion raising container (3); Be respectively equipped with the interface corresponding with taking over (2) at basis raising container (1) and expansion raising container (3), and be equipped with air-vent (4) at basis raising container (1) and expansion raising container (3).

4. general flour mite purifying raising device according to claim 3, it is characterized in that: take over (2) and be the pyramidal structure of hollow, the end that its diameter is large is connected to the basis and raises on the container (1), and the end that its diameter is little is connected to expansion and raises on the container (3).

5. general flour mite purifying raising device according to claim 4, it is characterized in that: raise container (3) in expansion and connect another expansion raising container (3) by taking over (2), the larger diameter end of taking over (2) is connected near the basis raises on the expansion raising container (3) of container (1).