CN103004697B - General flour mite purifying and feeding method and device - Google Patents
General flour mite purifying and feeding method and device Download PDFInfo
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- CN103004697B CN103004697B CN201210420366.5A CN201210420366A CN103004697B CN 103004697 B CN103004697 B CN 103004697B CN 201210420366 A CN201210420366 A CN 201210420366A CN 103004697 B CN103004697 B CN 103004697B
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Abstract
The invention discloses a general flour mite purifying and feeding method and a general flour mite purifying and feeding device. The method comprises the following steps of: at a purifying stage, placing a feed into a basic feeding container, and inoculating a female oogenesis mite or a pair of mating mites into the basic feeding container for feeding; after the flour mite(s) in the basic feeding container bear young, communicating the basic feeding container with an extension feeding container filled with a feed through a connecting tube, and entering an extension stage till the flour mites in the basic feeding container enter the extension feeding container automatically through the connecting tube; and after the flour mites finish moving, removing the connecting tube, sealing the interface of the extension feeding container, and taking the flour mites for later use to finish collection of the flour mites. A way for communicating the basic feeding container with the extension feeding container through the connecting tube is adopted, and flour mites actively enter a new environment due to taxis according to a designed route after foods are reduced and the environment is deteriorated, so that the aim of controlling the moving route of flour mites is fulfilled, and damages and losses of mite bodies during manual replacement of feeders and mite body movement are avoided.
Description
Technical field
The present invention relates to biological research fields, especially a kind of general flour mite purifying raising method and device.
Background technology
Room and reserve flour mite are the large monoids of one in flour mite, and their kinds are many, and distribution is wide, is the Major Members in edifice ecosystem.Its feeding habits widely, can endanger the grain of storage, traditional Chinese medicine, dry fruit, fabric etc., these article qualities be declined, destroys even completely.Room and reserve flour mite can multiply in a large number under suitable temperature and humidity conditions, can pollute the living environment of people, its excreta, secretion, slough off under skin and the lysate of dead body are all very strong allergens, the multiple allergic disease of people can be caused; Wherein some kind survival ability is very strong, if after entering human body by certain approach, can survive, cause acariasis in human body at people's endobiosis.Therefore, room and reserve flour mite are the insect of harm reserve, are again the pathogene of harm humans health.
Room and reserve flour mite are owing to endangering comparatively greatly, and day by day receive the concern of people, its research work also extensively and is rapidly being carried out.In addition, utilize flour mite as feed, produce Predatory Mites and carry out the important channel that biological control is also development green agriculture.No matter which kind of scientific research, its most basic basis is the acquisition of experimental subjects or experiment material.Otherwise any research work is all water without a source and a tree without roots.For the research object that build is small, experiment material except a few studies can by natural world collection obtain except (when needing quantity more, purebred being difficult to obtains by gathering separation), major part still by raising the material that obtain purifying, thus will ensure authenticity and the reliability of result of study.In the raising of mite class, forefathers have carried out a lot of exploration, have invented the individual culture apparatus as individual feeding device.This kind of device has really played important effect in individual feeding, but it is just cumbersome when expanding cultured space more further, need artificially to move mite body under the microscope, not only operating difficulties, and easily cause damage and the loss of mite body, purifying product quantity natively few in number is reduced, is unfavorable for expanding and cultivates.
Summary of the invention
The object of the invention is: provide a kind of general flour mite purifying raising method and device, it can not only raise mite kind by purifying, and workload is little, not easy damaged and loss mite body, and the equipment configuration adopted is simple, easy and simple to handle, to overcome the deficiencies in the prior art.
The present invention is achieved in that general flour mite purifying raising method, in purification phase, raises in container put into feed on basis, and female for oogenesis mite one or a pair is just raised in mating mite access basis raising container; After the flour mite raised in container until basis produces offspring, basis is raised container and raise reservoir by adapter and the expansion being placed with feed, entering extension phase, waiting for that the flour mite in basis raising container (determines to expand expansion quantity and the volume of feeder) by adapter from being advanced in expansion raising container according to required mite amount; After flour mite has been moved, remove adapter, sealed the interface that container is raised in expansion, take the collection namely completing flour mite for subsequent use away.
When needs conservation, in the raising container being placed with feed, put into the mite kind being no more than and raising vessel volume 1/3, breeding amount exceed raise container contents long-pending 1/2 time, connect by adapter the raising container that another is placed with feed; Repeat above-mentioned steps and realize conservation.
General flour mite purifying raising device, comprises basis and raises container, raising container in basis raises container by adapter with expansion and is connected; Basis raising container and expansion raising container are respectively equipped with and take over corresponding interface, and be equipped with air-vent on basis raising container and expansion raising container.
Adapter is the pyramidal structure of hollow, and one end that its diameter is large is connected to basis and raises on container, and one end that its diameter is little is connected to expansion and raises on container.Adapter adopts pyramidal structure that flour mite can be made easily to enter expansion raising container from basis raising container, and little owing to connecting the one end expanding raising container, the flour mite entering extension container is difficult to climb back basic feeder or the expansion feeder near basic feeder one end.
Raise on container in expansion and raise container by another expansion of adapter connection, the expansion that the larger diameter end of adapter is connected near basis raising container is raised on container.As required can infinite expanding cultured space.
Owing to have employed technique scheme, compared with prior art, the present invention adopts adapter to be communicated with basis and raises container and expand the mode of raising container, utilize flour mite taxis, make flour mite after food minimizing and environmental deterioration, initiatively can enter in new environment according to design route, thus reach the object controlling flour mite behavior route.Because the foreign material such as the mite corpse, ight soil and the cot under sloughing off that produce in the raising stage pile up generation poisonous and harmful substance for a long time, mite body can be affected healthy, therefore, prior art needs regularly artificial mobile flour mite usually, clear up its feeding environment, the present invention then can effectively address this problem, and avoids artificial to change the injury and loss that cause mite body in feeder and mobile mite body process; And receive mite conveniently, directly take the container of aequum away; Because cultured space can infinitely be expanded within the scope of needs, therefore suitable long-term conservation, saves storage area, is convenient to transport and exchanges, and reduces heavy subculture work, convenient operation, even layman also can operate.Reduce the waste of human and material resources resource unnecessary in production operation; By changing the size of raising container, some stored product insect feeder can be expanded to.The inventive method is simple, and be easy to industrialization, result of use is good.
Accompanying drawing explanation
Accompanying drawing 1 is structural representation of the present invention;
Accompanying drawing 2 is expansion structure schematic diagram of the present invention.
Embodiment
Embodiments of the invention: the structure of general flour mite purifying raising device as shown in Figure 1, comprises basis and raises container 1, raising container 1 in basis raise container 3 by adapter 2 with expansion and are connected; Raise on container 3 at basis raising container 1 and expansion and be respectively equipped with the interface corresponding with adapter 2, and be equipped with air-vent 4 on basis raising container 1 and expansion raising container 3; Adapter 2 is the pyramidal structure of hollow, and one end that adapter 2 diameter is large is connected to basis and raises on container 1, and one end that its diameter is little is connected to expansion and raises on container 3; Can raise on container 3 in expansion as required and connect another expansion raising container 3 by adapter 2, the larger diameter end of adapter 2 is connected to the expansion of raising container 1 near basis and raises on container 3; Profit in this way, makes cultured space can infinitely expand within the scope of needs.As shown in Figure 2.
The making of container 1 is raised on basis: adopt 0.2ml centrifuge tube, bottom alcolhol burner is baked soft, make the flat tubule that length is reduced to its former length 1/2, the hole that diameter is greater than 2mm is pricked in the side of body, and stick black filter paper with ventilative, namely make basis and raise container 1, based on the mouth of pipe one-tenth of centrifuge tube, raise the interface of container 1.
The making of adapter 2: adopt the pipettor gun head of 1ul (model can select the pipettor gun head of different model in proportion according to the size of expansion feeder) as adapter 2.
The making of container 3 is raised in expansion: can need to select according to volume in 0.5ml(model) bottom of centrifuge tube gets a hole to adapter 2, become the interface that container 3 is raised in expansion, the hole that diameter is greater than 2mm is pricked in the side of body, and stick black filter paper with ventilative, namely make expansion and raise container 3; The expansion finally finished up is raised container 3 and is adopted centrifuge tube to make equally, but its diameter raises container 3 slightly larger than expansion before, and with the mouth of pipe as its interface.
General flour mite purifying raising method:
Step one, purification phase: raise in container 1 on basis and put into a small amount of feed, and access the female mite of oogenesis one or a pair and just raise mating mite.For preventing natural death or peerless, multiple repetition can be established; Be put in control environment;
Step 2, extension phase: by observing, treat that the flour mite that basis is raised in container 1 produces offspring, open the capping that the interface of container 1 is raised on basis, receive on this interface by an end large for adapter 2 diameter, the other end of adapter 2 inserts expansion and raises in the interface of container 3; Along with increasing of mite amount in basis raising container 1, by adapter 2, container 3 is raised in multiple expansion and be communicated with to get up, as shown in Figure 2; The wait basis flour mite raised in container 1 climbs into expansion voluntarily by adapter 2 and raises in container 3, and when observation basis is raised movable without mite body in container 1 and adapter 2, removal adapter 2, seals the interface that container 3 is raised in expansion with black filter paper.
Step 3, collection phase: container 3 is raised in the expansion choosing suitable output according to required mite amount, and directly takes away for subsequent use after sealing its interface, also directly can take off ending feeder 7 for subsequent use.
Step 4, conservation stage: the expansion that optional step three is collected raises container 3 as initial feeder, after flour mite automatic moving completes, remove first expansion and raise container 3 and adapter 2; Continue to raise after a period of time, when mite density is larger, then get the new centrifuge tube being placed with feed and make object feeder, former object feeder then becomes initial feeder.Repetition above-mentioned steps.The conservation stage also can establish multiple repetition.
Claims (4)
1. a general flour mite purifying raising method, is characterized in that: in purification phase, raises in container put into feed on basis, and female for oogenesis mite one or a pair is just raised in mating mite access basis raising container; After the flour mite raised in container until basis produces offspring, basis being raised container and raise reservoir by adapter and the expansion being placed with feed, enter extension phase, treating that the flour mite that basis is raised in container is raised in container by adapter from being advanced into expansion; After flour mite has been moved, remove adapter, sealed the interface that container is raised in expansion, take the collection namely completing flour mite for subsequent use away.
2. general flour mite purifying raising method according to claim 1, it is characterized in that: when needs conservation, the mite kind being no more than and raising vessel volume 1/3 is put in the raising container being placed with feed, breeding amount exceed raise container contents long-pending 1/2 time, connect by adapter the raising container that another is placed with feed; Repeat above-mentioned steps and realize conservation.
3. a general flour mite purifying raising device, comprises basis and raises container (1), it is characterized in that: on basis raising container (1), raise container (3) by adapter (2) with expansion be connected; Raise on container (3) on basis raising container (1) and expansion and be respectively equipped with the interface corresponding with taking over (2), and be equipped with air-vent (4) on basis raising container (1) and expansion raising container (3); The pyramidal structure that adapter (2) is hollow, one end that its diameter is large is connected to basis and raises on container (1), and one end that its diameter is little is connected to expansion and raises on container (3).
4. general flour mite purifying raising device according to claim 3, it is characterized in that: raise on container (3) in expansion and connect another expansion raising container (3) by adapter (2), the larger diameter end of adapter (2) is connected to raises in expansion raising container (3) of container (1) near basis.
Priority Applications (1)
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CN201210420366.5A CN103004697B (en) | 2012-10-29 | 2012-10-29 | General flour mite purifying and feeding method and device |
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CN201210420366.5A CN103004697B (en) | 2012-10-29 | 2012-10-29 | General flour mite purifying and feeding method and device |
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CN103004697A CN103004697A (en) | 2013-04-03 |
CN103004697B true CN103004697B (en) | 2015-04-22 |
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CN105557641B (en) * | 2015-12-24 | 2018-02-06 | 吉林农业大学 | A kind of method and device collected with the soft cocoon honeybee of child care gelechiid |
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CN101049097A (en) * | 2007-03-15 | 2007-10-10 | 福建农林大学 | Conservation method for parent species of predatory mites |
EP2136619B1 (en) * | 2007-03-30 | 2011-06-08 | ALK-Abelló A/S | A method for mite production |
CN201290295Y (en) * | 2008-10-28 | 2009-08-19 | 北京市农林科学院 | Multifunctional splicing type animals and plants living-body cultivation cage |
GB0900117D0 (en) * | 2009-01-06 | 2009-02-11 | Certis Europ B V | Apparatus for rearing and releasing of predatory mites and accommodating their prey |
CN101773099B (en) * | 2010-01-29 | 2013-11-13 | 张艳璇 | Method for artificial feeding production of Steinman small blind cucumeris |
CN101904319A (en) * | 2010-06-28 | 2010-12-08 | 赣南脐橙技术工程研究中心 | Predatory mite bait and amblyseius barkeri seed protecting bottle and seed protecting method |
CN102106318A (en) * | 2011-01-17 | 2011-06-29 | 广东出入境检验检疫局检验检疫技术中心 | Culture device for white fly and tetranychid mite feeding experiment and method for conducting experiment using same |
CN201986546U (en) * | 2011-01-21 | 2011-09-28 | 邵连鹏 | Cascading combined type ant homeland device |
CN202857586U (en) * | 2012-10-29 | 2013-04-10 | 贵州大学 | Simple and efficient flour mite purification breeding device |
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