CN115885932A - Method for directionally breeding high-temperature resistant corpus luteum strain of gelechiid smith - Google Patents
Method for directionally breeding high-temperature resistant corpus luteum strain of gelechiid smith Download PDFInfo
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- CN115885932A CN115885932A CN202211443705.1A CN202211443705A CN115885932A CN 115885932 A CN115885932 A CN 115885932A CN 202211443705 A CN202211443705 A CN 202211443705A CN 115885932 A CN115885932 A CN 115885932A
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- 238000009395 breeding Methods 0.000 title claims abstract description 28
- 230000001488 breeding effect Effects 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 23
- 210000004246 corpus luteum Anatomy 0.000 title claims abstract description 20
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 claims abstract description 57
- 229960004441 tyrosine Drugs 0.000 claims abstract description 29
- 235000012907 honey Nutrition 0.000 claims abstract description 24
- 241000256011 Sphingidae Species 0.000 claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 241001454728 Gelechiidae Species 0.000 claims description 12
- 230000000938 luteal effect Effects 0.000 claims description 10
- 241000256856 Vespidae Species 0.000 claims description 9
- 235000015097 nutrients Nutrition 0.000 claims description 8
- 241001125149 Molossus Species 0.000 claims description 5
- 241000255945 Choristoneura Species 0.000 claims description 4
- 241000142468 Bracon Species 0.000 claims 1
- 230000003071 parasitic effect Effects 0.000 abstract description 8
- 241000209140 Triticum Species 0.000 abstract description 6
- 235000021307 Triticum Nutrition 0.000 abstract description 6
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 abstract description 2
- 241000382353 Pupa Species 0.000 abstract 1
- 241000256816 Braconidae Species 0.000 description 6
- 241000257303 Hymenoptera Species 0.000 description 5
- 230000024241 parasitism Effects 0.000 description 4
- 230000001902 propagating effect Effects 0.000 description 4
- 230000019617 pupation Effects 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 235000013162 Cocos nucifera Nutrition 0.000 description 3
- 244000060011 Cocos nucifera Species 0.000 description 3
- 241000985295 Habrobracon hebetor Species 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- 241000195940 Bryophyta Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
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- 241000255967 Helicoverpa zea Species 0.000 description 1
- 241000255777 Lepidoptera Species 0.000 description 1
- 208000007256 Nevus Diseases 0.000 description 1
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- 241001414987 Strepsiptera Species 0.000 description 1
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- 229940024606 amino acid Drugs 0.000 description 1
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- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a method for directionally breeding a high-temperature resistant corpus luteum strain of a hornworm molocos, which comprises the steps of feeding the hornworm molocos into adult hornworms by honey water mixed with L-tyrosine, parasitizing to obtain offspring, and treating at 30-32 ℃ in the pupa generation period of the hornworm molocos to obtain the high-temperature resistant corpus luteum strain of the hornworm molocos. By adopting the method, the high-temperature resistant wheat moth Roughty cocoon bee with corpus luteum and high temperature resistance can be obtained, the high-temperature resistant wheat moth Roughty cocoon bee strain can be bred at higher temperature, the indoor and outdoor parasitic rates are obviously higher than those of common strains, and the unique golden yellow body color ensures that the high-temperature resistant strain has obvious specificity compared with the common strains, is easier to distinguish and is beneficial to the identification of the strain.
Description
Technical Field
The invention relates to the technical field of insect breeding, in particular to a method for directionally breeding a corpus luteum high-temperature-resistant strain of a hornworm molossus.
Background
A Choristoneura braconica Hebetor, latin name Habrobracon hebetor, is an insect belonging to the genus Choristonella of the family Hymenoptera braconidae, widely distributed in China and all over the world, and belongs to a parasitic insect. The bracon hebetor can parasitize larvae of various lepidoptera insects such as coco moths, cotton bollworms and the like, and is used for controlling related pests.
Female hornworm of hornworm: brown yellow body and black antenna; the single-eye area, the back head, the middle part of the face, the middle chest back plate, the middle chest side plate and the chest-abdominal joint are dark brown; transparent wings, semi-dark brown base, and wing nevi and wing veins. Abdomen 3, back plate and back plate are yellow brown; the sheath is dark brown. Males are female-like, with elongated tentacles, and the 1 st segment of the flagellum is slightly longer than the 2 nd segment. The abdominal parts 1 and 2 (except the arched triangular part of the section 1) are gray yellow. The adult body color of the gelechiid smith shows brown yellow, the body color is uneven, and the gelechiid smith has mixed colors, so that no relevant report about breeding of the luteal gelechiid smith is found at present.
Research shows that the parasitism rate of the bracon hebetor is closely related to the temperature, the parasitism rate is higher in the range of 26-29 ℃, and when the temperature is further increased, the parasitism rate is reduced. For example, dianbaozhu et al studied the paralytic and parasitic effects of the Choristoneura heterospodum on 5 th larvae of Indian meal moth at different densities in the room at 17, 23, 26, 32 and 35 ℃. The results show that: the host processing time of parasitic wasps is inversely proportional to temperature in the range of 17-29 deg.c. The host search rate also varies with temperature
Lowest at 17 ℃ and highest at 29 ℃; when the temperature is increased to 32-35 ℃, the host search rate is reduced again. Therefore, strict temperature control is required for propagation of the hornworm molossus in production. However, the southern area of China has high temperature all the year round, so the characteristic of the hornworm of the wheat moth which can not resist high temperature restricts the popularization and the application of the hornworm of the wheat moth in the southern area. In view of the above, there is a need for further breeding of a high temperature resistant strain of hornworts maid. At present, the breeding work of the high-temperature resistant strains of the hornworts has not been effectively progressed, and no relevant report is found.
Disclosure of Invention
In view of the defects of the prior art, the application provides a method for directionally breeding a high-temperature resistant corpus luteum strain of the hornworm molossus.
The scheme of the invention is as follows:
a method for directionally breeding a high-temperature resistant corpus luteum strain of a Choristoneura heterospodum comprises the following steps:
feeding the adult hornworm molocos into a nutrient solution containing an L-tyrosine solution, parasitizing to obtain offspring, and treating the offspring of the pupal stage hornworm molocos at 30-32 ℃ to obtain the corpus luteum high-temperature-resistant strain of the hornworm molocos.
Preferably, the nutrient solution containing the L-tyrosine solution is a sugar source containing L-tyrosine.
Preferably, the nutrient solution containing the L-tyrosine solution is honey water containing L-tyrosine.
Preferably, the mass ratio of L-tyrosine, honey and water in the nutrient solution is 1-5.
Preferably, the mass ratio of the L-tyrosine to the honey to the water is 1.
Preferably, the treatment time at 30 to 32 ℃ is 48 to 72 hours.
Preferably, the method for directionally breeding the high-temperature resistant corpus luteum strain of the gelechiid braconidae comprises the following steps:
feeding the hornet mollissima into honey water mixed with L-tyrosine, parasitizing to obtain offspring, and treating the hornet mollissima offspring in the pupal stage at 30 ℃ for 48 hours to obtain the high-temperature resistant strain of the corpus luteum of the hornet mollissima.
Preferably, the honey is Chinese bee honey.
Compared with the prior art, the invention has the beneficial effects that:
the invention firstly utilizes honey water mixed with L-tyrosine to feed the gelechiid braconid wasps to form the adult wasps, opens a regulation and control path, and then further carries out treatment for 48 to 72 hours at 30 to 32 ℃ in the pupal stage of offspring. Under the comprehensive action of the steps, the high-temperature resistant corpus luteum strain of the wheat moth bombyx batryticatus is obtained. The high-temperature-resistant wheat moth cocoon bee strain can be propagated at a higher temperature, the indoor and outdoor parasitic rates are obviously higher than those of common strains, and the unique golden yellow body color enables the high-temperature-resistant strain to have obvious specificity compared with the common strains, so that the strain is easier to distinguish and is beneficial to the identification of the strain.
The high-temperature resistant strain bred by the method disclosed by the invention can be more suitable for high-temperature climate, and the efficiency of preventing and controlling the palm pests, namely coconut leaf moths and the like, in Hainan is higher.
The method disclosed by the invention is simple and convenient to operate, does not need expensive instruments or reagents, is low in cost and short in treatment time, and can be used for stably obtaining the corpus luteum high-temperature-resistant strain of the hornworm molossus within a short period of time.
Drawings
FIG. 1: comparative figures for different gelechiid braconidae strains. The left graph is the strain which can not resist high temperature, and the right graph is the strain which can resist high temperature after the breeding of the invention.
Detailed Description
In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.
Example 1 method for directionally breeding high-temperature resistant luteal strain of gelechiid smith
The adult hornets were fed with honey water mixed with L-tyrosine (L-tyrosine: honey: water = 1. Propagating the gelechiid moths soft cocoon bee by using the gelechiid moths as hosts, and treating the gelechiid moths soft cocoon bee by using 30 ℃ (the relative humidity is 60 +/-10%) for 48 hours in the pupation stage of the gelechiid moths soft cocoon bee seeds to obtain high-temperature resistant offspring gelechiid moths soft cocoon bee with yellow body color.
Comparing the high temperature resistant strain with the non-high temperature resistant strain before breeding, the new strain bred has golden body color, less and light mixed color, and the strain before breeding has obvious yellow brown or black body surface and more mixed color. (FIG. 1)
Test example: high temperature resistance capability test of different gelechiid braconid beetle strains
The indoor test procedure is carried out according to the influence of different hosts on the parasitism, development and propagation of the hornet of the Maifand moth (Chen Yunlei, yan Sanqiang, lvbouqiang, luhui, tang-Secondary flood and Wangchong). The host is coconut fruit moth.
And (3) field test: the indoor propagation of the imagoes of the gelechiid braconid moths is carried out according to the bee-keeping quantity of 12000 heads/hm 2 Releasing bees twice at an interval of 7 days, and keeping the average temperature above 30 ℃ during releasing bees. The plant species planted in the test field and the cultivation management mode thereof are the same. The number of the larvae of hundreds of coconuts and the moths in the field is investigated once before the bees are put, each treatment is divided into 3 districts, each district is investigated for 5 points, and each point is investigated for 10 plants. For the second timeAnd 7 days after the bees are placed, bringing the larvae back indoors for observation, counting the parasitic quantity of the pests, and calculating the parasitic rate.
And comparing the parasitic rates of the high-temperature resistant strain and the non-high-temperature resistant strain, wherein the result shows that the indoor and outdoor parasitic rates of the high-temperature resistant strain are obviously higher than those of the control strain. (see table below).
A large number of experiments prove that the breeding method can be used for stably obtaining the corpus luteum high-temperature resistant strain. In the breeding process, the temperature can be selected from 30-32 ℃, the treatment time can be selected from 48-72 hours, and the above effects can be achieved through verification. As shown in examples 2-3.
Example 2 method for directionally breeding high-temperature resistant luteal strain of gelechiid smith
Adult hornworms and molossid were fed with honey water mixed with L-tyrosine (L-tyrosine: honey: water = 1. And (3) expanding and propagating the gelechiid smith as a host, and treating the gelechiid smith with 32 ℃ (the relative humidity is 60 +/-10%) for 48 hours in the pupation stage of the gelechiid smith to obtain high-temperature resistant progeny gelechiid smith with yellow body color.
Embodiment 3 method for directionally breeding high-temperature resistant luteal strain of gelechiid smith
Adult hornworms and molossid were fed with honey water mixed with L-tyrosine (L-tyrosine: honey: water = 1. And (3) expanding and propagating the gelechiid smith as a host, and treating the gelechiid smith with 30 ℃ (the relative humidity is 60 +/-10%) for 72 hours in the pupation stage of the gelechiid smith to obtain high-temperature resistant progeny gelechiid smith with yellow body color.
The treatment at 30-32 ℃ is strictly controlled in the pupal stage, and the high-temperature resistant strain can be obtained without inducing the color change of parasitic bees in other stages.
Only tyrosine can initiate the regulatory pathways, and other amino acids have no effect. Therefore, feeding honey water mixed with L-tyrosine is a key element for obtaining the corpus luteum high-temperature resistant strain. Wherein, L-tyrosine: honey: the mass ratio of water is (1-5) to 10. As shown in example 4.
Example 4 method for directionally breeding high-temperature resistant luteal strain of gelechiid smith
Adult hornworms and molossid were fed with honey water mixed with L-tyrosine (L-tyrosine: honey: water = 5. Propagating the gelechiid moths soft cocoon bee by using the gelechiid moths as hosts, and treating the gelechiid moths soft cocoon bee for 72 hours at the pupation stage of the gelechiid moths soft cocoon bee seeds at 30 ℃ (the relative humidity is 60 +/-10%) to obtain high-temperature resistant offspring gelechiid moths soft cocoon bee with yellow body color.
The above description is only exemplary of the present invention and should not be taken as limiting the invention, and any modifications, equivalents, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (8)
1. A method for directionally breeding a high-temperature resistant corpus luteum strain of a Choristoneura heterospodum is characterized by comprising the following steps:
feeding the adult hornworm molocos into a nutrient solution containing an L-tyrosine solution, parasitizing to obtain offspring, and treating the offspring of the pupal stage hornworm molocos at 30-32 ℃ to obtain the corpus luteum high-temperature-resistant strain of the hornworm molocos.
2. The method for directionally breeding the high-temperature resistant luteal line of the gelechiid smith coir according to claim 1, wherein the nutrient solution containing the L-tyrosine solution is a sugar source containing L-tyrosine.
3. The method for directionally breeding the high-temperature resistant luteal line of the gelechiid smith coir according to claim 1, wherein the nutrient solution containing the L-tyrosine solution is honey water containing L-tyrosine.
4. The method for directionally breeding the high-temperature resistant line of the corpus luteum of the gelechiidae condoms according to claim 3, wherein the mass ratio of L-tyrosine, honey and water in the nutrient solution is 1-5.
5. The method for directionally breeding the high-temperature resistant luteal line of the gelechiid condoms according to claim 4, wherein the mass ratio of L-tyrosine, honey and water is 1.
6. The method for directionally breeding the corpus luteum high-temperature-resistant line of the gelechiidae condoms according to claim 1, wherein the treatment time at 30-32 ℃ is 48-72 hours.
7. The method for directionally breeding the high-temperature resistant luteal line of the gelechiid smith bracon as claimed in claim 1, comprising the following steps:
feeding the hornet mollissima into honey water mixed with L-tyrosine, parasitizing to obtain offspring, and treating the hornet mollissima offspring in the pupal stage at 30 ℃ for 48 hours to obtain the high-temperature resistant strain of the corpus luteum of the hornet mollissima.
8. The method for directionally breeding the high-temperature resistant luteal strain of the hornworm molossus according to claim 1, wherein the honey is Chinese bee honey.
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| CN202211443705.1A CN115885932A (en) | 2022-11-18 | 2022-11-18 | Method for directionally breeding high-temperature resistant corpus luteum strain of gelechiid smith |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| UA25137U (en) * | 2007-03-27 | 2007-07-25 | Univ Nat Agrarian | Method of rearing the ectoparasite habrobracon hebetor say. |
| CN104026083A (en) * | 2014-06-26 | 2014-09-10 | 湖南农业大学 | Method for selectively breeding high-temperature-tolerant earthworms |
| CN105557641A (en) * | 2015-12-24 | 2016-05-11 | 吉林农业大学 | Method and device for collecting and conserving habrobracon hebetor |
| CN109997791A (en) * | 2019-04-08 | 2019-07-12 | 西南大学 | A kind of new small peaceful mite of Pasteur strain development resistant to high temperature and identification method |
| CN110367203A (en) * | 2019-06-06 | 2019-10-25 | 贵州省烟草公司贵阳市公司 | A kind of artificial batch feeding packaging microbracon hebetor living body at bee method |
| CN111887207A (en) * | 2020-07-09 | 2020-11-06 | 武汉市园林科学研究院 | Application of paralytic function of gelechiid chogramma in artificial cultivation of natural enemy insects |
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- 2022-11-18 CN CN202211443705.1A patent/CN115885932A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| UA25137U (en) * | 2007-03-27 | 2007-07-25 | Univ Nat Agrarian | Method of rearing the ectoparasite habrobracon hebetor say. |
| CN104026083A (en) * | 2014-06-26 | 2014-09-10 | 湖南农业大学 | Method for selectively breeding high-temperature-tolerant earthworms |
| CN105557641A (en) * | 2015-12-24 | 2016-05-11 | 吉林农业大学 | Method and device for collecting and conserving habrobracon hebetor |
| CN109997791A (en) * | 2019-04-08 | 2019-07-12 | 西南大学 | A kind of new small peaceful mite of Pasteur strain development resistant to high temperature and identification method |
| CN110367203A (en) * | 2019-06-06 | 2019-10-25 | 贵州省烟草公司贵阳市公司 | A kind of artificial batch feeding packaging microbracon hebetor living body at bee method |
| CN111887207A (en) * | 2020-07-09 | 2020-11-06 | 武汉市园林科学研究院 | Application of paralytic function of gelechiid chogramma in artificial cultivation of natural enemy insects |
Non-Patent Citations (2)
| Title |
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| 吴琦琦: "营养、温度对椰子织蛾三种寄生蜂基础生物学的影响", 《中国优秀硕士学位论文全文数据库 农业科技辑》, no. 6, 15 June 2020 (2020-06-15), pages 4 - 8 * |
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