CN113287574A - Method for propagating blue Changdou gold wasps - Google Patents
Method for propagating blue Changdou gold wasps Download PDFInfo
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- CN113287574A CN113287574A CN202110479349.8A CN202110479349A CN113287574A CN 113287574 A CN113287574 A CN 113287574A CN 202110479349 A CN202110479349 A CN 202110479349A CN 113287574 A CN113287574 A CN 113287574A
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Animal Behavior & Ethology (AREA)
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Abstract
The invention provides a method for propagating blue Changdou gold wasps, which comprises the following steps: (1) selecting rubber seedlings planted to be 80-100cm in height; (2) collecting the primarily hatched nymphs of Erysipela pelykii or Erysipelothrus pelykii, inoculating the nymphs to rubber saplings for cultivation, and growing to adults laying eggs for 5-6 d; (3) placing the cultured host plant with the adult Lecanicillium acornis Hemsl in a bee-inoculating cage at 30-33 ℃, inoculating the parasitic wasps according to the quantity ratio of 1 (30-40) to the host of the adult, and controlling the photoperiod L: D to be 14h, 10 h-16 h and 8 h; (4) taking out the host plant with the coccid, culturing at 30-33 ℃, controlling the photoperiod L to be 12h, 12 h-14 h and 10h, collecting and rejuvenating; the invention can realize the high-efficiency propagation of the blue Changbai gold wasps under the high-temperature condition, and has the advantages of high egg laying amount, high parasitic efficiency, high population growth and propagation speed and high survival rate.
Description
Technical Field
The invention relates to the technical field of biological control of agricultural and forestry pests, in particular to a method for propagating blue Changdingjin wasps.
Background
The scale insects are important pests on a plurality of economic crops in China at present. Scale insects which harm rubber trees in China include lecanicillium, mealybugs, pelagic and the like, and the lecanicillium is one of important groups. The pests mainly use imagoes and nymphs to prick and suck the juice of leaves and twigs of host plants, so that the branches and leaves are yellow, fallen leaves, branches are dry and withered, and the like; the insect also secretes honeydew to induce host plants to generate soot disease, which affects photosynthesis of plants, and if the plant is small, the host plants are hindered in growth and reduced in yield, and if the plant is heavy, the whole plant is withered. Ericerus pela Parasalacia nigra Nietner, Ericerus pelagitans Ceroplasts rubens Maskeel, Ericerus pelagitans Coccus hesperidum Linnaeus, elevicola Saisseria oleva (Bernard), and Ericerus pelagitans Ceroplasts floridens Comstock, all of which have caused significant economic losses to the rubber industry, have been reported to harm rubber trees.
At present, the prevention and control of the lecanicillium insect mainly depends on chemical agents at home and abroad. However, the body of the lecanicillium lecanii pest has thick wax shells, so the total effect of chemical control is not ideal. Secondly, with the forbidding of efficient organophosphorus pesticides for scale insects such as methidathion, omethoate and the like, the use of low-toxicity pesticides such as dimethoate, chlorpyrifos and the like is limited, the types of the available medicaments for chemical control of the lecanicillium insect are less and less, and the control effect is also poorer and less. In addition, the application of chemical pesticides not only affects the quality safety of products, but also threatens the ecological safety, for example, the use of high-toxicity pesticides easily destroys the biodiversity, kills a large amount of beneficial organisms or neutral insects, destroys the ecological balance and easily causes outbreak and disaster of various diseases and insects. Therefore, the prevention and control of the pests of the lecanicillium scale needs to have new technology and product breakthrough.
The natural enemy resources available for preventing and controlling the scale insects are quite rich in the natural world, wherein the parasitic natural enemy insects are an important natural enemy group. The continuous prevention and control of scale insects by using parasitic wasps is a new technical direction, and some successful cases are found at home and abroad, for example, the cercaria gambieri and the like are successfully used in Fujian province, Guangdong province and the like to successfully prevent and control the pine greedy scale, and the cercaria gambieri and the like are used in Hainan to effectively prevent and control the rubber-paraselys lecanii and the cercaria gambieri within a certain temperature range. In the research and development aspect of natural enemy propagation technology, the method mainly aims at the dominant parasitic wasps of Erysipela pelykii: two kinds of endoparasitic bees, namely Coccophagus japonica company (ZL201610584035.3) and Ananadis comatus diversnervus elegans Silvestri (ZL201110092539.2), of the Japanese icerya aphids are developed and are applied to a small production range. Nevertheless, the growth suitable temperature of the two parasitic wasp populations is within 21-27 ℃, the release in the high-temperature glue planting area in summer is limited, and the biological control in the tropical area is greatly limited.
The blue Changdou golden wasp Scutellaria caerulea (Fonscolombe) is an important parasitic wasp of the lecanidae insects. The main hosts of the plant comprise olive Hemiasterculus Saissetia oleae Olivier, Ceratococcus flavus floridensis Comstock, Ceratococcus flavus russianus Linnaeus, Coccus heperidum Linnaeus, and Paracoccus ruber Hessi Nietner. The bee is an ectoparasitic adult parasitic bee, eggs are laid on the ventral surface of the lecanium scale from a gap between the lecanium scale and a host plant mainly through a spawning needle, and the larvae of the wasps feed on the eggs laid under the abdomen of the lecanium scale adult, so that the natural population quantity of the existing wasps in the field is low, and the natural pest control effect on pests cannot be achieved. At present, artificial propagation and utilization are not seen at home and abroad, but the traditional artificial propagation method of parasitic wasps cannot realize stable high-temperature parasitic efficiency and egg laying amount, has low survival rate in the development process and low propagation expansion efficiency, so that the invention provides the high-efficiency artificial propagation method suitable for the blue Changdinghua wasps, improves the population growth and propagation speed under the high-temperature condition, realizes effective utilization of the blue Changdinghua wasps, and has important significance for improving the control effect of preventing and controlling the scale insects in tropical regions.
Disclosure of Invention
In view of the above, the invention provides a rapid artificial propagation method suitable for the blue Changbai gold wasps, which can realize the efficient propagation of the blue Changbai gold wasps under the high-temperature condition, and has the advantages of high egg laying amount, high parasitic efficiency, high population growth propagation speed and high survival rate.
The technical scheme of the invention is realized as follows:
a method for propagating blue Changdou gold wasps comprises the following steps:
the method comprises the following steps: selecting a host plant: selecting rubber seedlings as host plants, and planting the rubber seedlings in a pot to a height of 80-100cm for later use;
step two: cultivating adult hosts: collecting the nymphs hatched initially of Erysipelothrix peltatus or Erysipelothrix lutea, inoculating the nymphs to rubber tree seedlings for cultivation until the adults lay eggs for 5-6d for later use;
step three: high-temperature bee receiving: taking the blue Changdou golden vespid as parasitic wasps, putting the host plants with the adult Lecanicillium acornissioides bred in the step two into a wasp inoculating cage at the temperature of 30-33 ℃, inoculating the wasps according to the number ratio of 1 (30-40) of female wasps and adult hosts of the parasitic wasps, and controlling the photoperiod L: D: 14h: 10-16 h:8 h;
step four: after the bee inoculation is completed, taking out the host plant with the coccid, culturing at the temperature of 30-33 ℃, controlling the photoperiod to be L: D: 12h: 12-14 h:10h, collecting parasitic bee products, and rejuvenating the parasitic bee species in the field.
More preferably, in the second step, the initially hatched nymph of the elaeis canicola is adopted for breeding the adult host, and the adult of the elaeis canicola is adopted as the host, so that the parasitic efficiency of the elaeis canicola is improved better, and the egg laying amount is increased.
More preferably, in the third step, the humidity of the bee receiving is 60% -70%, and the bee receiving time is 2-3 d.
More preferably, in step three, the parasitic female wasp is a wasp prepared from the following raw materials in a ratio of 1: 1, the female wasp and the male wasp of the blue Changdou gold apis cerana are concentratedly mated for 1 day at the temperature of 30-33 ℃, and the survival and the egg laying capacity of the parasitic wasp in high-temperature inoculation can be favorably improved.
More preferably, in the fourth step, the humidity of the culture is 60% -70%, and the culture time is 15-18 d.
Further explaining, in the fourth step, when the blue Changdou gold wasps develop to the pupation rate of 90% -100%, stripping a part of parasitic wasp pupae, putting the wasp pupae into a drying oven at 33 ℃ for drying moisture or blowing dry the moisture by electric air blowing for field release, putting the other part into a bee cage for continuous culture, and adding honey or cane sugar aqueous solution with the mass fraction of 18-22% into the cage for a new round of bee expansion when the pupae eclosion becomes adult bees.
More preferably, in the fourth step, 40-60% of wild blue Changding gold wasps are added every 3-4 months or indoor propagated healthy and strong blue Changding gold wasps are selected as bee species for rejuvenation.
More preferably, in the third step, the volume of the bee catching cage is 400cm × 300cm × 180cm, and the bee catching cage is isolated by gauze.
Compared with the prior art, the invention has the beneficial effects that: the invention effectively realizes the high-temperature propagation of the blue Changbai gold vespids, wherein rubber seedlings with certain height are used as host plants, the rubber parakok or the olive black helmet scale of the lecanidae is used as an intermediate host of the golden vespids for breeding the golden vespids, the parasitism force of the golden vespids to the olive black helmet scale, the rubber parakok, the limousine and the like is effectively kept, meanwhile, the invention adopts the host in the adult stage and combines the high-temperature bee-grafting mode to control the bee-grafting proportion and the photoperiod condition of the parasitic wasps, the high-temperature egg yield and the survival rate of the blue Changbai gold vespids are greatly improved, the parasitic efficiency and the survival rate in the development process of the parasitic wasps are further improved by utilizing the high-temperature culture condition and the photoperiod condition, the high-temperature rapid propagation of the blue Changbai gold vespids is realized, the blue Changbai gold vespids are better released in a high-temperature rubber planting area in summer, and the slow high-temperature parasitic rate is effectively solved, the survival rate is low, the population growth and propagation speed is low, the population growth can be higher at the temperature of more than 33 ℃, the supply and demand of biological control are ensured, and the control effect in forests is improved.
The blue Changbai golden vespid can be released in the adult stage of the lecanidae, or jointly released with parasitic wasps such as Japanese Ichnocarpus opercularis, elegans, paraglobin, paragecko podocarpus and the like when various insect states of the lecanidae exist, so that the parasitic rates of the lecanidae such as olive black army scale, rubber paragecko, brown soft scale and the like are improved better, and the control effect on the population quantity of pests is improved.
Detailed Description
In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.
The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.
The materials, reagents and the like used in the examples of the present invention can be obtained commercially without specific description.
Example 1
A method for propagating blue Changdou gold wasps comprises the following steps:
(1) selecting a host plant:
selecting rubber seedlings as host plants, and planting the rubber seedlings in a pot to a height of 80-100cm for later use;
(2) cultivating adult hosts:
collecting the primarily hatched nymphs of the Ericerus pela, inoculating the collected primarily hatched nymphs to rubber seedlings for cultivation, wherein the cultivation temperature is 26 ℃, forming a test population taking the rubber seedlings as hosts, and poking the leaves of the rubber seedlings once a day within one week to ensure that the primarily hatched nymphs of the Ericerus pela on each leaf of the rubber seedlings are uniformly distributed until the adult insects lay eggs for 5-6d in the black period of the Ericerus pela are developed for later use; one part is used for inoculating parasitic wasps, and the other part is used for continuing the development of the parasitic wasps and is reserved as a propagation seed source of the Erysipela peltata;
(3) high-temperature bee receiving:
selecting large-individual and strong-vitality blue scolyptera pellegelii as parasitic wasps, putting the host plants of the adult Lecanicillium acornissioides bred in the step two into a wasp inoculating cage with the temperature of 30 ℃ and the humidity of 60-65%, inoculating the wasps according to the quantity ratio of the female wasps of the parasitic wasps to the hosts of the adult wasps of 1:30, and controlling the photoperiod L: D: 14h:10 h; after inoculating the bees for 2d, blowing off blue adult scutellum pellegelii on the rubber seedlings by using a blower;
(4) high-temperature culture:
after the bee inoculation is finished, taking out the host plant with the coccid of the bee inoculation, culturing for 16D in a culture room with the temperature of 30 ℃ and the humidity of 60-65%, controlling the photoperiod L:12 h, stripping a part of parasitic bee pupae from the surface of a leaf by using a blade when the blue Changdingjin xiaosha grows to the pupation rate of 90%, drying the parasitic bee pupae in a drying oven with the temperature of 33 ℃, packaging and storing or directly using the parasitic bee pupae and a female bee for field release, continuously culturing the other part of the blue Changdingjin xiaosha in a bee inoculation cage, and adding a honey water solution with the mass fraction of 18% into the cage for a new round of bee expansion when the pupae emerges into adult bees; adding 40% of wild blue Changdou gold wasps as bee species into the bee cage every 3 months for rejuvenation.
The specification of the bee receiving cage is a stainless steel frame with the length, width and height of 400cm 300cm 180cm, the middle of the bee receiving cage is divided into 4 by a wood plate partition (each stainless steel frame with the length, width and height of 400cm 300cm 45cm is isolated by a gauze and provided with an independent illumination system), the top surface of the bee receiving cage is sealed by glass, the back surface and the side surface of the bee receiving cage are sealed by 150 meshes, the front surface of the bee receiving cage is provided with two semicircular sliding doors, the two sliding doors are sealed by the gauze, and the operation in the breeding process is carried out through the doors, which is the same as the following steps.
And (3) nutrition supplement: the honey water with the concentration of 20% is prepared to be used for supplementing nutrition for the blue Changdong golden wasp imagoes.
Example 2
A method for propagating blue Changdou gold wasps comprises the following steps:
(1) selecting a host plant:
(2) selecting rubber seedlings as host plants, and planting the rubber seedlings in a pot to a height of 80-100cm for later use;
(2) cultivating adult hosts:
collecting the primarily hatched nymphs of the black armyworm, inoculating the nymphs to rubber seedlings for cultivation, wherein the cultivation temperature is 26 ℃, forming a test population taking the rubber seedlings as hosts, and shifting the rubber seedling leaves once a day within one week to ensure that the primarily hatched nymphs of the black armyworm on each leaf of the rubber seedlings are uniformly distributed until the adults lay eggs for 5-6 days in the black period of the black armyworm for later use; one part is used for inoculating parasitic wasps, and the other part is used for continuing to develop and is reserved as a breeding seed source of the black mealybugs;
(3) high-temperature bee receiving:
selecting large-individual and strong-vitality blue Scomberochloes pelagi as parasitic wasps, and carrying out free mating at 30 ℃ according to the ratio of 1 female wasp to 1 male wasp to obtain female wasps; placing the host plant with the adult elaeis canicola bred in the step two into a bee-inoculating cage with the temperature of 33 ℃ and the humidity of 60-65%, inoculating the parasitic wasps according to the number ratio of 1:40 of the female wasps of the parasitic wasps to the adult hosts, and controlling the photoperiod L: D: 16h:8 h; after inoculating the bees for 2d, blowing off blue adult scutellum pellegelii on the rubber seedlings by using a blower;
(4) high-temperature culture:
after the bee inoculation is finished, taking out the host plant with the coccid of the bee inoculation, putting the host plant into a cultivation room with the temperature of 30 ℃ and the humidity of 60-65% for cultivation for 16D, controlling the photoperiod L to be 12h to 12h, stripping a part of parasitic bee pupae from the surface of a leaf by using a blade when the blue Changdingjin xiaosha grows until the pupation rate is 100%, putting the parasitic bee pupae into a drying oven with the temperature of 33 ℃ for drying moisture, packaging and storing or directly using the parasitic bee pupae and a female bee for field release, putting the other part into a bee inoculation cage for continuous cultivation, and adding a sucrose aqueous solution with the mass fraction of 22% into the cage for a new round of bee expansion when the pupae emerges into adult bees; indoor strong and vigorous blue Changbai gold vespis individuals which are propagated in the room are added into the bee-inoculating cage every 4 months and are taken as bee species for rejuvenation.
And (3) nutrition supplement: and (3) preparing cane sugar water with the concentration of 20% for supplementing nutrition for the blue Changdong golden wasp imagoes.
Example 3
A method for propagating blue Changdou gold wasps comprises the following steps:
(1) selecting a host plant:
selecting rubber seedlings as host plants, and planting the rubber seedlings in a pot to a height of 80-100cm for later use;
(2) cultivating adult hosts:
collecting the primarily hatched nymphs of the black armyworm, inoculating the nymphs to rubber seedlings for cultivation, wherein the cultivation temperature is 26 ℃, forming a test population taking the rubber seedlings as hosts, and shifting the rubber seedling leaves once a day within one week to ensure that the primarily hatched nymphs of the black armyworm on each leaf of the rubber seedlings are uniformly distributed until the adults lay eggs for 5-6 days in the black period of the black armyworm for later use; one part is used for inoculating parasitic wasps, and the other part is used for continuing to develop and is reserved as a breeding seed source of the black mealybugs;
(3) high-temperature bee receiving:
selecting large-individual and strong-vitality blue Scomberochloes pelagi as parasitic wasps, and carrying out free mating at 33 ℃ according to the ratio of 1 female wasp to 1 male wasp to obtain female wasps; placing the host plant with the bred adult elaucides olivaceus and black mealybugs in the second step into a bee-inoculating cage with the temperature of 33 ℃ and the humidity of 60-70%, inoculating the parasitic wasps according to the ratio of the number of the female wasps of the parasitic wasps to the number of the adult hosts of 1:35, and controlling the photoperiod to be L: D: 14h:10 h; after inoculating the bees for 2d, blowing off blue adult scutellum pellegelii on the rubber seedlings by using a blower;
(4) high-temperature culture:
after the bee inoculation is finished, taking out the host plant with the coccid of the bee inoculation, culturing for 16D in a culture room with the temperature of 33 ℃ and the humidity of 60-70%, controlling the photoperiod L: D: 14h:10h, stripping a part of parasitic bee pupae from the surface of the leaf by using a blade when the blue Changdingjin wasp grows until the pupation rate is more than 95%, drying the parasitic bee pupae in a drying oven with the temperature of 33 ℃, packaging and storing or directly using the parasitic bee pupae and a female bee for field release, continuously culturing the other part of the blue Changdingjin wasp in a bee-inoculating cage, and adding a honey aqueous solution with the mass fraction of 20% into the cage for a new round of bee expansion when the pupae emerges into adult bees; adding 40-60% of wild blue Changdong gold wasps as bee seeds into the bee cage every 3 months for rejuvenation.
And (3) nutrition supplement: the honey water with the concentration of 20% is prepared to be used for supplementing nutrition for the blue Changdong golden wasp imagoes.
Example 4
This example differs from example 3 in that: in step (3), female bees are obtained by free mating at 28 ℃ in a ratio of 1 female bee to 1 male bee, and the rest is the same as in example 3.
According to the expanding propagation method of the blue Changbai gold vespids in the embodiments 1-4, the egg laying amount of the small vespids and the number of parasitized coccid of the small vespids 24 hours after the vespids are inoculated are respectively counted, the parasitizing rate of the small vespids is calculated, and the peak emergence number and the female rate of the small vespids after the blue Changbai gold vespids are cultured at a high temperature for 16 days are counted.
TABLE 1 parasitism and reproduction of different blue Changdong gold wasps
The result shows that the blue Changbai gold wasps can realize high-temperature bee-grafting at the temperature of 30-33 ℃, the egg laying amount of the blue Changbai gold wasps in 24 hours is more than 30, the parasitic rate can reach 95.2% to the maximum, the bee output in 16 days is more than 15, the male rate can reach more than 50%, and the male rate in the embodiment 3 can reach 65.15%.
To further verify the beneficial effects of the present invention, the following comparative verification tests were performed:
1. oviposition and parasitic rate of blue Changbai gold wasps at different bee-receiving temperatures
And (3) experimental setting: the parasitic wasps of example 3 were taken and set in 5 experimental groups with temperature gradients of 24 ℃, 27 ℃, 30 ℃, 33 ℃, 35 ℃ and a photoperiod of 14L according to example 3: and 10D, respectively inoculating the bees for 24 hours in the same proportion in an artificial climate box with the humidity of 60-70%, taking out the artificial climate box, dissecting and observing the egg laying amount of the vespids and the number of parasitic coccid, and calculating the parasitic rate of the vespids.
Table 2 spawning and parasitizing rates of blue Changbai gold wasps at different bee-inoculating temperatures
The result shows that the blue Changbai gold wasp can realize high-temperature bee inoculation at the temperature of 30-33 ℃, the egg laying amount is obviously increased, and the parasitic rate at the temperature of 33 ℃ can reach 90.8%.
2. Oviposition and parasitic rate of blue Changbai gold wasps in different photoperiods
And (3) experimental setting: taking the parasitic wasps of example 3, the photoperiod was set as: l: d is 2:22, 4:20, 6:18, 8:16, 10:12, 12:12, 14:10, 16:8, 18:6, 20:4, 22:2 and 24:0 photoperiod, inoculating bees for 24 hours in an artificial climate box with the root inoculating bee temperature of 33 ℃ and the humidity of 60-70% respectively according to the same proportion, taking out the eggs laid by the dissected bee and observing the number of the parasitized coccid, and calculating the parasitizing rate of the bee bees.
TABLE 3 spawning and parasitism rates of blue Changbai gold wasps at different photoperiods
The results show that: the blue Changbai gold wasps do not lay eggs under the complete dark condition, and the egg laying amount and the parasitic rate are increased along with the increase of the illumination time. The egg laying amount reaches more than 45.0 grains when the sunlight duration is more than 16h, and the parasitic rate can reach more than 80% when the sunlight duration is more than 12 h.
3. Oviposition and parasitic rate of blue Changbai gold wasps in different host ages
And (3) experimental setting: in a small area of a rubber sapling, 20 heads of adult insects in the brown period of the black armyworm, black adult insects (egg laying 1-2d of the scale insect), black adult insects (egg laying 5-6d of the scale insect) and black adult insects (egg laying 9-10d of the scale insect) of the black armyworm are respectively selected for standby, the parasitic wasps in the embodiment 3 are taken, and the wasps are received in an artificial climate box with the temperature of 33 ℃ and the humidity of 60-70% according to the wasp receiving method in the embodiment 3, and the light period is 14L: and (5) taking out the wasps 24 hours later, dissecting and observing the egg laying amount of the wasps and the number of parasitized coccid, and calculating the parasitizing rate of the wasps.
TABLE 4 spawning and parasitism rates of blue Changbai gold wasps of different host ages
From the above table, it can be seen that: the influence of the host development stage on the egg laying of the blue Changbai gold wasps is obvious, the parasitic rate and the egg laying amount are increased along with the increase of the development stage in the range of the initial adult-black stage adult, the highest egg laying rate (5-6 d) in the black stage is 43.3 grains and 95.6 percent respectively, and the adult Lecanicillium abruptum which lays eggs for 5-6d is preferably adopted as an intermediate host during propagation.
4. Development and propagation of blue Changdou gold wasps at different bee-inoculating temperatures
And (3) experimental setting: the high temperature bee inoculation according to the embodiment 3, then the coccid inoculated with the bee are respectively put under the conditions of 24 ℃, 27 ℃, 30 ℃, 33 ℃ and 35 ℃ to be cultured under the condition of controlling the photoperiod L: D as 14h:10h and the humidity of 60-70% as the embodiment 3, and the development duration, the bee output, the sex ratio and the like of the vespids are observed.
TABLE 5 blue Changbai gold wasp development and reproduction at different bee-inoculating temperatures
The results show that: in the range of 24-33 ℃, the development period of the blue Changbai gold vespid is gradually shortened along with the increase of the temperature, the number of the grown bees is gradually increased, and the blue Changbai gold vespid is the highest at 33 ℃; the female rate is higher at 30-35 ℃, and reaches 65.71% at 33 ℃, which shows that the parasitic wasp can realize effective propagation at 30-33 ℃.
5. Development and propagation of blue Changbai gold wasps under different photoperiods
And (3) experimental setting: the high-temperature bee inoculation is carried out according to the embodiment 3, then the coccid inoculated with the bee is respectively placed into the conditions of 8L:16D, 10L:12D, 12L:12D, 14L:10D and 8L:16D for 5 photoperiods, the temperature is controlled to be 33 ℃ and the humidity is 60-70 percent as in the embodiment 3, and the development duration, the bee production amount, the sex ratio and the like of the young bees are observed.
TABLE 6 development and reproduction of blue Changbai gold wasps under different photoperiods
The results show that: within the range of 8L to 16D to 14L to 10D, the development duration of the blue Changbai gold wasps is gradually shortened along with the extension of the illumination time, and the number of the wasps is increased; the female rate is the highest at 8L:16D and is 68.47%, the female rate is 14L:10D, the quantity, the development speed, the female rate and other parameters of the bees are combined, and the selecting of 14L:10D during the propagation of the small gold bees is most beneficial to improving the propagation efficiency.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (8)
1. A method for propagating blue Changdou gold wasps is characterized by comprising the following steps: the method comprises the following steps:
the method comprises the following steps: selecting a host plant: selecting rubber seedlings planted to be 80-100cm in height as host plants for later use;
step two: cultivating adult hosts: collecting the nymphs hatched initially of Erysipelothrix peltatus or Erysipelothrix lutea, inoculating the nymphs to rubber tree seedlings for cultivation until the adults lay eggs for 5-6d for later use;
step three: high-temperature bee receiving: taking the blue Changdou golden vespid as parasitic wasps, putting the host plants with the adult Lecanicillium acornissioides bred in the step two into a wasp inoculating cage at the temperature of 30-33 ℃, inoculating the wasps according to the number ratio of 1 (30-40) of female wasps and adult hosts of the parasitic wasps, and controlling the photoperiod L: D: 14h: 10-16 h:8 h;
step four: after the bee inoculation is completed, taking out the host plant with the coccid, culturing at the temperature of 30-33 ℃, controlling the photoperiod to be L: D: 12h: 12-14 h:10h, collecting parasitic bee products, and rejuvenating the parasitic bee species in the field.
2. The method for propagating blue scutellariae Sinapis as claimed in claim 1, wherein: and in the second step, the initially hatched nymphs of the black mealybugs are adopted to cultivate adult hosts.
3. The method for propagating blue scutellariae Sinapis as claimed in claim 1, wherein: in the third step, the bee receiving humidity is 60% -70%, and the bee receiving time is 2-3 d.
4. The method for propagating blue scutellariae Sinapis as claimed in claim 1, wherein: in the third step, the parasitic wasp female is prepared by mixing the following components in a ratio of 1: 1, the female bee and the male bee of the blue Changdou gold vespids are gathered and freely mated for 1 day at the temperature of 30-33 ℃.
5. The method for propagating blue scutellariae Sinapis as claimed in claim 1, wherein: in the fourth step, the humidity of the culture is 60% -70%, and the culture time is 15-18 d.
6. The method for propagating blue scutellariae Sinapis as claimed in claim 1, wherein: in the fourth step, when the blue Changbai gold vespids develop to the pupation rate of 90% -100%, stripping a part of parasitic bee pupae, putting the parasitic bee pupae into a drying oven at 33 ℃ for drying water or blowing the water by electric air blowing for field release, putting the other part into a bee inoculating cage for continuous culture, and when the pupae emerge into adult bees, adding 18-22% by mass of honey or sucrose aqueous solution into the cage for a new round of bee expanding.
7. The method for propagating blue Changdou gold vespids as claimed in claim 6, wherein: in the fourth step, 40-60% of wild blue Changding gold wasps are added every 3-4 months or indoor propagated healthy and strong blue Changding gold wasps are selected as bee species for rejuvenation.
8. The method for propagating blue scutellariae Sinapis as claimed in claim 1, wherein: in the third step, the volume of the bee receiving cage is 400cm multiplied by 300cm multiplied by 180cm, and the bee receiving cage is isolated by a gauze.
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| WO2022227359A1 (en) * | 2021-04-30 | 2022-11-03 | 中国热带农业科学院环境与植物保护研究所 | Method for propagating scutellista caerulea fonscolombe |
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