CN108770790A - A kind of Dendrolimus kikuchii method for breeding - Google Patents
A kind of Dendrolimus kikuchii method for breeding Download PDFInfo
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- CN108770790A CN108770790A CN201810247318.8A CN201810247318A CN108770790A CN 108770790 A CN108770790 A CN 108770790A CN 201810247318 A CN201810247318 A CN 201810247318A CN 108770790 A CN108770790 A CN 108770790A
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- 241000301144 Dendrolimus kikuchii Species 0.000 title claims abstract description 38
- 238000009395 breeding Methods 0.000 title claims abstract description 19
- 230000001488 breeding effect Effects 0.000 title claims abstract description 19
- 238000000034 method Methods 0.000 title claims abstract description 17
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 22
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 22
- 210000004681 ovum Anatomy 0.000 claims abstract description 22
- 241000382353 Pupa Species 0.000 claims abstract description 12
- 241000238631 Hexapoda Species 0.000 claims abstract description 8
- 230000001418 larval effect Effects 0.000 claims abstract description 8
- 241000607479 Yersinia pestis Species 0.000 claims description 54
- 239000004677 Nylon Substances 0.000 claims description 37
- 229920001778 nylon Polymers 0.000 claims description 37
- 235000013305 food Nutrition 0.000 claims description 18
- 239000011521 glass Substances 0.000 claims description 16
- 235000013601 eggs Nutrition 0.000 claims description 13
- 230000012447 hatching Effects 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 229920000297 Rayon Polymers 0.000 claims description 7
- 238000004140 cleaning Methods 0.000 claims description 7
- 239000002964 rayon Substances 0.000 claims description 7
- 230000007613 environmental effect Effects 0.000 claims description 6
- 230000037406 food intake Effects 0.000 claims description 6
- 239000003755 preservative agent Substances 0.000 claims description 5
- 230000002335 preservative effect Effects 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 4
- 241000012249 Dendrolimus spectabilis Species 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims description 2
- 235000011613 Pinus brutia Nutrition 0.000 claims description 2
- 241000018646 Pinus brutia Species 0.000 claims description 2
- 238000012545 processing Methods 0.000 claims description 2
- 239000013589 supplement Substances 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 abstract description 7
- 238000011160 research Methods 0.000 abstract description 7
- 230000004083 survival effect Effects 0.000 abstract description 6
- 238000011161 development Methods 0.000 abstract description 4
- 238000002474 experimental method Methods 0.000 abstract description 2
- 230000000717 retained effect Effects 0.000 abstract 1
- 235000005205 Pinus Nutrition 0.000 description 4
- 241000218602 Pinus <genus> Species 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 3
- 238000007598 dipping method Methods 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- UPLPHRJJTCUQAY-WIRWPRASSA-N 2,3-thioepoxy madol Chemical compound C([C@@H]1CC2)[C@@H]3S[C@@H]3C[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@](C)(O)[C@@]2(C)CC1 UPLPHRJJTCUQAY-WIRWPRASSA-N 0.000 description 1
- BVPWJMCABCPUQY-UHFFFAOYSA-N 4-amino-5-chloro-2-methoxy-N-[1-(phenylmethyl)-4-piperidinyl]benzamide Chemical compound COC1=CC(N)=C(Cl)C=C1C(=O)NC1CCN(CC=2C=CC=CC=2)CC1 BVPWJMCABCPUQY-UHFFFAOYSA-N 0.000 description 1
- 241001544353 Barringtonia racemosa Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241001631715 Dendrolimus Species 0.000 description 1
- 241001631712 Dendrolimus pini Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000218648 Keteleeria Species 0.000 description 1
- 241000961578 Keteleeria fortunei Species 0.000 description 1
- 241000255679 Lasiocampidae Species 0.000 description 1
- 235000011609 Pinus massoniana Nutrition 0.000 description 1
- 241000018650 Pinus massoniana Species 0.000 description 1
- 241000048269 Pinus taiwanensis Species 0.000 description 1
- 206010035148 Plague Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000035613 defoliation Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000008093 supporting effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of method for breeding of Dendrolimus kikuchii, belong to technical field of insect feeding.The present invention includes the raising of ovum phase, larval phase, pupa time and complete worm state phase in adult stage four, for each worm phase of Dendrolimus kikuchii development, take different breeding technologies, the raising survival rate of each worm phase is positively retained at 90% or more, ensure that the continuity of the stability and related scientific research experiment of worm sources.The present invention provides a kind of method for breeding of system, ripe Dendrolimus kikuchii, and support is provided for the basic and applied research of Dendrolimus kikuchii.
Description
Technical field
The invention belongs to technical field of insect feeding, and in particular to a kind of Dendrolimus kikuchii method for breeding.
Background technology
Dendrolimus kikuchiiDendrolimus kikuchiiMatsumura belongs to Lepidoptera Lepidoptera Lasiocampidaes
Lasiocampid- ae are one of the leafeating insects for endangering most serious of south China pine tree.Host includes pinus khasysPinus langhianensisChev., pinus yunnanensisP. yunnanensisFranch, Huashan pineP. armandi Franch, horse hair
PineP. massonianaLambert, Pinus taiwanesis (taiwanensis)P. taiwanensis, Pinus fenzelianaP. fenzeliana、
Yunnan keteleeria fortuneiKeteleeria evelvnianaMast., arbor-vitaePlatycladus orientalisEndl., cypressCupressus funebrisEndl, deodarCedrus deodaraDeng.China be mainly distributed on Yunnan, Guizhou, Sichuan,
The provinces such as Guangdong, Guangxi, Hubei, Hunan, Jiangsu, Zhejiang, Anhui, Jiangxi, Fujian, Taiwan, Hainan.
From its host and distribution can, it is evident that its host endangered be China south China, southwest it is most important,
Widest coniferous species are distributed, and almost entire southern area is distributed.The weight of its harm and distribution are wide, have drawn
Extensive concern and the research of numerous researchers and forestry practitioner are played.Such as Zhou Jianhua in article, " give birth to by Dendrolimus kikuchii
History, habit and controlling experiment research living "（Forestry in Guizhou Province science and technology 1989,17 (2)：31-36）In disclose " indoor observation be use
Iron wire cylinder mould 20 (high 25cm, long 30cm, wide 20cm), often worm one is adopted from woods away from 10 plants, will be loose per cage worm one
In the sub- cage of stem cutting in bottle, going out within 2 days, it is primary to change；It is provided with three wire mesh cages (high l00cm, long 40cm, wide 40cm), choosing in outdoor
The masson pine treelet suitable with cage height is selected, tree is entangled on the spot, every cage worm 5.The 4-10 months, observation was primary daily, November-the
In March, 2, observation was primary per half a month." Zhang Chaoju is in article " Dendrolimus kikuchii biological characteristics and study on prevention "（East China insect
Journal, 2002,11（2）:74-78）In mention " in annual early June from woodland adopt back pieces of an egg a batch, be placed on indoor glass culture
In ware, individual feeding 100 is divided after larvae hatch, 1-2 ages, which are placed in dactylethrae, raises, and is placed in culture dish and raises after 3 ages
It supports, replaces 1 feed within every 2 days；Group breeding every group 20 is placed in raising in plastic circular insect box (diameter 25cm, height
30cm), each worm state habit is observed day by day；Each worm state feature is described." bavin keep Quan Deng " Dendrolimus kikuchii larva defoliation mount measure
And the development of prediction plague of insects series table "（Forestry science and technology communicates, and 2001,11:6-8）In refer to that " raising of indoor vial is just
Larva 30 is incubated, is compiled with 1~No. 30, is put into fresh Yunnan keteleeria fortunei blade daily, removes excrement and clast, and measure a secondary eclipse leaf
Amount."
Dendrolimus kikuchii is holometabolan, including four ovum, larva, adult and pupa worm states, wherein larva generally comprise 7 ages
Phase.Simao worm's ovum is more sensitive to environment, and environment can influence the hatching rate of ovum, the change of larval phase environment, especially humidity
Variation, is affected to pine moth larval mortality.Therefore each worm state and the rearing conditions of larva whole story phase are different, it
The raising Dendrolimus kikuchii of preceding this field, just for certain several worm phase, or more general raising, supporting effect can be impacted,
Survival rate is relatively low.There are no a system, maturation, the higher Dendrolimus kikuchii method for breeding of survival rate up to now, for correlation
Basic and applied research provide standard worm sources, this is also that it wreaks havoc harm for a long time, the major reason that cannot be effectively controlled
One of.
Invention content
For case above, those skilled in the art endeavour to provide a kind of system, ripe Dendrolimus kikuchii method for breeding,
Support is provided for the basic and applied research of Dendrolimus kikuchii.
The technical solution that the present invention takes is：
A kind of Dendrolimus kikuchii method for breeding includes the raising of ovum phase, larval phase, pupa time and complete worm state phase in adult stage four,
It includes glass culture dish, absorbent cotton, preservative film, gauze and nylon box for feeding pests to raise the article and implement used.Feeding process is：
（1）The ovum phase raises
Dendrolimus kikuchii ovum is put into glass culture dish, blank space puts the rayon balls of a moistening and anhydrous spilling in ware,
It is 50-60% to keep humidity in ware, and glass culture dish is put in interior, is raised under room temperature, and observation is until Simao pine tag daily
Egg hatch, egg hatching time are 9-14 days；
（2）Larval phase, is raised
A. first-instar young is raised：The raising of first-instar young carries out in the culture dish of hatching, under room temperature, observes that glass is trained
When having egg hatching in foster ware, the tender branch of acquisition host after being handled, is put into 2-3,5-10cm host's branch in each ware,
It is 40-70% to keep humidity in ware, and timing daily is observed, and the host's branch more renewed according to food ingestion clears up swill in time
And frass, ensure cleaning in food supply and ware；
B. second instar larvae is raised：Second instar larvae writing brush or hairbrush are displaced in nylon box for feeding pests, two ages in each box for feeding pests
Larva quantity is 200-500 heads, is put in interior, and the tender branch of acquisition host is handled, is put into the 10-30cm handled well and posts
The gauze of several layers of water-soaked is placed in 3-5, major branch, nylon box for feeding pests top and side, and it is 40- to keep humidity in box for feeding pests
70%, timing daily is observed, and the host's branch more renewed according to food ingestion clears up swill and frass in time, ensures that food supplies
Should with cleaned in box for feeding pests；
C. third-instar larvae is raised：Each nylon box for feeding pests is put into third-instar larvae 200, is raised according to second instar larvae condition；
D. four-age larva is raised：Each nylon box for feeding pests is put into four-age larva 50, is raised according to second instar larvae condition；
E. five-age larva is raised：Each nylon box for feeding pests is put into five-age larva 30；It is raised according to second instar larvae condition；
F. six instar larvaes are raised：Each nylon box for feeding pests is put into six instar larvae 30；It is raised according to second instar larvae condition；
G. seven instar larvaes are raised：Each nylon box for feeding pests is put into seven instar larvae 30, is raised according to second instar larvae condition, also
It needs to place 2-3 in nylon box for feeding pests and dry up host's branch without leaf, adhere to for mature larva and do cocoon；
（3）Pupa time raises
In cocoon, cocoon is hung on branch or box for feeding pests wall the pupa of Dendrolimus kikuchii, careful to remove other sundries in nylon box for feeding pests,
It not interfere and move pine caterpillar, routine observation ensures the moistening of nylon box for feeding pests periphery gauze, and humidity is in box for feeding pests
50-60%；
（4）Adult stage raises
The adult that cocoon-break is sprouted wings presses sex ration 1-2：1 is put into same nylon insect cage case, is put in interior, is placed in box for feeding pests
The host of 3-5 20-30cm long with leaf branch item, spray a little clear water to branches and leaves moistening and it is anhydrous drip, supplement feeding for adult,
And 2-3 withered host's branches without leaf are placed simultaneously for Adult worms producting eggs, routine observation ensures branches and leaves moistening.
Further, a diameter of 150mm of glass culture dish used in the ovum phase raising.
Further, the ovum phase raises, and the Dendrolimus kikuchii ovum number being put into each glass culture dish is 200-500
It is a.
Further, host's branch processing described in larva raising, is the tender branch of the host that will acquire, is rushed with clear water
It washes, dries, in the branch root rayon balls package for dipping in water, then with preservative film environmental sealing.
Further, the nylon box for feeding pests be 35cm × 35cm × 35cm square, nylon wire be 100 mesh with
On.
In feeding process, environmental condition especially humidity influences very big, the too low development for being unfavorable for ovum of humidity to survival rate
Hatching, humidity, which crosses conference, causes worm's ovum to go mouldy, and influences hatching rate.Larval phase humidity is excessive or food is replaced not in time or excrement is clear
Reason not in time, can lead to food spoilage, environmental pollution, catch an illness and dead so as to cause Dendrolimus kikuchii, at the same time, food
It replaces and excrement cleaning can excessively bother the development that can influence worm（As casted off a skin, sprouting wings）, in feeding process, each glass culture dish
Or in box for feeding pests worm quantity it is also very crucial, quantity can lead to the wasting of resources very little, can cause too much food replace it is excessively frequent.
Therefore, the present invention chooses suitable environmental condition, suitable stocking density, properly according to the different growing periods of Dendrolimus kikuchii
Breeding technology, only need to change food daily and cleaning excrement be primary, ensure that the survival rate of each period raising.
Beneficial effects of the present invention are：This breeding technology includes four ovum of Dendrolimus kikuchii, larva, pupa and adult worms
The state phase takes different breeding technologies according to each worm phase, is raised at ambient temperature to Dendrolimus kikuchii, each worm phase
Raising survival rate 90% or more, ensure that the stability of Dendrolimus kikuchii worm sources, for Dendrolimus kikuchii related scientific research work carry
Strong support is supplied.
Specific implementation mode
A kind of Dendrolimus kikuchii method for breeding includes ovum, larva, pupa and the complete worm state of adult four of Dendrolimus kikuchii
The raising of phase, specifically includes：
（1）The ovum phase raises：By Dendrolimus kikuchii ovum, 300 are put into diameter 150mm glass culture dish, and blank space puts one in ware
The rayon balls of moistening and anhydrous spilling, it is 50-60% to keep humidity in ware, glass culture dish is put in interior, under room temperature
Raising, observation daily is until Dendrolimus kikuchii egg hatching, egg hatching time are 9-14 days；
（2）Larva raising
First-instar young is raised：The tender branch of host is acquired, washes with water, dries, in the branch root rayon balls packet for dipping in water
It wraps up in, then with preservative film environmental sealing, observes in glass culture dish when having egg hatching, be put into treated 10cm host's branch
3, it is 40-70% to keep humidity in ware, and timing daily is observed, and the host's branch more renewed according to food ingestion clears up food in time
Residue and frass ensure cleaning in food supply and ware
Second instar larvae is raised：Second instar larvae writing brush or hairbrush are displaced in 35cm × 35cm × 35cm nylon box for feeding pests, Buddhist nun
More than 100 mesh of imperial net, second instar larvae quantity is 300 in each box for feeding pests, is put in interior, and the tender branch of acquisition host uses water
Cleaning, dries, and in the branch root rayon balls package for dipping in water, then with preservative film environmental sealing, each box for feeding pests is put into place
The gauze of several layers of water-soaked is placed in 5, the 20cm host's branch managed, nylon box for feeding pests top and side, keeps wet in box for feeding pests
Degree is 40-70%, and timing daily is observed, and the host's branch more renewed according to food ingestion clears up swill and frass in time, protects
Demonstrate,prove cleaning in food supply and box for feeding pests；
Third-instar larvae is raised：Each nylon box for feeding pests is put into third-instar larvae 200, is raised according to second instar larvae condition；
Four-age larva is raised：Each nylon box for feeding pests is put into four-age larva 50, is raised according to second instar larvae condition；
Five-age larva is raised：Each nylon box for feeding pests is put into five-age larva 30；It is raised according to second instar larvae condition；
Six instar larvaes are raised：Each nylon box for feeding pests is put into six instar larvae 30；It is raised according to second instar larvae condition；
Seven instar larvaes are raised：Each nylon box for feeding pests is put into seven instar larvae 30, is raised according to second instar larvae condition, is also needed
3 are placed in nylon box for feeding pests to dry up host's branch without leaf, and cocoon is done for mature larva attachment；
（3）Pupa time raises
In cocoon, cocoon is hung on branch or box for feeding pests wall the pupa of Dendrolimus kikuchii, careful to remove other sundries in nylon box for feeding pests,
It not interfere and move pine caterpillar, routine observation ensures the moistening of nylon box for feeding pests periphery gauze, and humidity is in box for feeding pests
50-60%；
（4）Adult stage raises
It is put in interior, the host that 5 25cm long are placed in box for feeding pests sprays a little clear water and moistened to branches and leaves and anhydrous with leaf branch item
It drips, feeding is supplemented for adult, and place 3 withered host's branches without leaf simultaneously for Adult worms producting eggs, routine observation is protected
Demonstrate,prove branches and leaves moistening.
Claims (5)
1. a kind of Dendrolimus kikuchii method for breeding, which is characterized in that complete including ovum phase, larval phase, pupa time and adult stage four
The raising of worm state phase, specifically includes：
（1）The ovum phase raises
Dendrolimus kikuchii ovum is put into glass culture dish, blank space puts the rayon balls of a moistening and anhydrous spilling in ware,
It is 50-60% to keep humidity in ware, and glass culture dish is put in interior, is raised under room temperature, and observation is until Simao pine tag daily
Egg hatch, egg hatching time are 9-14 days；
（2）Larval phase, is raised
A. first-instar young is raised：The raising of first-instar young carries out in the culture dish of hatching, under room temperature, observes that glass is trained
When having egg hatching in foster ware, the tender branch of acquisition host after being handled, is put into 2-3,5-10cm host's branch in each ware,
It is 40-70% to keep humidity in ware, and timing daily is observed, and the host's branch more renewed according to food ingestion clears up swill in time
And frass, ensure cleaning in food supply and ware；
B. second instar larvae is raised：Second instar larvae writing brush or hairbrush are displaced in nylon box for feeding pests, two ages in each box for feeding pests
Larva quantity is 200-500 heads, is put in interior, and the tender branch of acquisition host is handled, is put into the 10-30cm handled well and posts
The gauze of several layers of water-soaked is placed in 3-5, major branch, nylon box for feeding pests top and side, and it is 40- to keep humidity in box for feeding pests
70%, timing daily is observed, and the host's branch more renewed according to food ingestion clears up swill and frass in time, ensures that food supplies
Should with cleaned in box for feeding pests；
C. third-instar larvae is raised：Each nylon box for feeding pests is put into third-instar larvae 200, is raised according to second instar larvae condition；
D. four-age larva is raised：Each nylon box for feeding pests is put into four-age larva 50, is raised according to second instar larvae condition；
E. five-age larva is raised：Each nylon box for feeding pests is put into five-age larva 30；It is raised according to second instar larvae condition；
F. six instar larvaes are raised：Each nylon box for feeding pests is put into six instar larvae 30；It is raised according to second instar larvae condition；
G. seven instar larvaes are raised：Each nylon box for feeding pests is put into seven instar larvae 30, is raised according to second instar larvae condition, also
It needs to place 2-3 in nylon box for feeding pests and dry up host's branch without leaf, adhere to for mature larva and do cocoon；
（3）Pupa time raises
In cocoon, cocoon is hung on branch or box for feeding pests wall the pupa of Dendrolimus kikuchii, careful to remove other sundries in nylon box for feeding pests,
It not interfere and move pine caterpillar, routine observation ensures the moistening of nylon box for feeding pests periphery gauze, and humidity is in box for feeding pests
50-60%；
（4）Adult stage raises
The adult that cocoon-break is sprouted wings presses sex ration 1-2：1 is put into same nylon insect cage case, is put in interior, is placed in box for feeding pests
The host of 3-5 20-30cm long with leaf branch item, spray a little clear water to branches and leaves moistening and it is anhydrous drip, supplement feeding for adult,
And 2-3 withered host's branches without leaf are placed simultaneously for Adult worms producting eggs, routine observation ensures branches and leaves moistening.
2. a kind of Dendrolimus kikuchii method for breeding according to claim 1, it is characterised in that：It is used in the ovum phase raising
The a diameter of 150mm of glass culture dish.
3. a kind of Dendrolimus kikuchii method for breeding according to claim 1 or 2, it is characterised in that：The ovum phase raising, often
The Dendrolimus kikuchii ovum number being put into a glass culture dish is 200-500.
4. a kind of Dendrolimus kikuchii method for breeding according to claim 1, it is characterised in that：Posting described in larva raising
The processing of major branch item is the tender branch of the host that will acquire, rinsed, dried with clear water, and in branch root, use dips in the rayon balls of water
Package, then with preservative film environmental sealing.
5. a kind of Dendrolimus kikuchii method for breeding according to claim 1, it is characterised in that：The nylon box for feeding pests is
The square of 35cm × 35cm × 35cm, nylon wire are 100 mesh or more.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110278922A (en) * | 2019-07-26 | 2019-09-27 | 贵州大学 | Allomyrina dichotoma artificial diet technique |
| CN112471079A (en) * | 2020-12-11 | 2021-03-12 | 兰州大学 | Indoor artificial feeding method for Qinghai-Tibet plateau grassland caterpillars |
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