CN109601485B - Simple method for rapidly collecting dichocrocis punctiferalis eggs - Google Patents
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- CN109601485B CN109601485B CN201811547977.XA CN201811547977A CN109601485B CN 109601485 B CN109601485 B CN 109601485B CN 201811547977 A CN201811547977 A CN 201811547977A CN 109601485 B CN109601485 B CN 109601485B
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The invention relates to the technical field of biology, in particular to a simple method for quickly collecting dichocrocis punctiferalis eggs. The method comprises the following steps: putting a proper amount of adult dichocrocis punctiferalis into a spawning cage, wherein the male-female ratio is 1:1-1.5, putting a culture dish at the bottom of the spawning cage, putting absorbent cotton dipped with cane sugar water into the culture dish, and covering and sealing the mouth of the spawning cage by absorbent cotton gauze; after the egg-laying cages are cultivated for 1-3 days, egg-laying media are put into the egg-laying cages, then distilled water is sprayed every day, the egg-laying media are replaced periodically, and the eggs are preserved and cultivated until all adult female dichocrocis punctiferalis adults die; and taking out the processed egg laying medium from the egg laying cage, and collecting the dichocrocis punctiferalis eggs. The method solves the problem that the larvae are manually transferred to the host plants in a time-consuming and labor-consuming manner when the larvae are hatched from eggs. The invention can quickly, simply and efficiently collect the dichocrocis punctiferalis eggs, saves time and labor, is convenient for counting the eggs falling on the outer gauze of the corn cob, and is also convenient for using the collected eggs to continue other tests.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a simple method for quickly collecting dichocrocis punctiferalis eggs.
Background
The dichocrocis punctiferalis can not only harm peach, apple, pear, Chinese chestnut and other fruit trees, but also harm corn, soybean, cotton and other crops, and is called as 'ten fruit nine moth' to the damage severity of peach, Chinese chestnut and other fruit trees, but is generally very light to the crops. With the improvement of crop planting structure and the popularization of fruit tree fruit bagging technology, the harm of dichocrocis punctiferalis gradually spreads from fruit trees to farmland crops, particularly to corn, and becomes an important pest in summer corn fields in Huang-Huai-Hai areas. In recent years, the damage severity degree of the dichocrocis punctiferalis to the ear of summer corn is even higher than that of the Asiatic corn borer, and the dichocrocis punctiferalis becomes a dominant species of the corn ear pests. Therefore, the enhancement of researches on the biology, the ecology, the occurrence and migration mechanism and the like of dichocrocis punctiferalis pests is of great significance for better making a control strategy for dichocrocis punctiferalis.
Previously, cotton gauze or fresh corn cobs were mainly used to collect eggs of dichocrocis punctiferalis. In the former, cotton gauze (40 multiplied by 30 cm) is sealed at the opening of an insect cage, after eggs are collected, the cotton gauze with the eggs and fresh corns are put into an insect box together for waiting for larvae to hatch, and because the odor of host plants is lacked to attract when the eggs are collected, the amount of the collected eggs is less, and the collection efficiency is low; although the latter collects eggs by using host plants liked by dichocrocis punctiferalis, the surface of the corn kernels is smooth and is not beneficial to the oviposition of dichocrocis punctiferalis (dichocrocis punctiferalis prefers to oviposit on a slightly rough surface of a substance, such as cotton gauze), so the collection efficiency is low, the color of the corn is similar to that of dichocrocis punctiferalis eggs, and the subsequent egg counting consumes time and labor. A research paper by Du Yan et al in 2012 describes a method for collecting eggs of dichocrocis punctiferalis using fresh apples coated with a layer of cotton gauze (insect bulletin, 55(5): 561-: apples are easy to mildew and rot, 1-2 days are needed for replacing spawning media for 1 time, and after the spawns are collected, the spawns need to be transferred from the spawning media to larva breeding plants (corns), and the like, so that the operation is time-consuming, labor-consuming and uneconomical; the application of Gunn Asia Li et al in 2018 discloses a method for collecting eggs of dichocrocis punctiferalis by using absorbent cotton, but the method has more problems: (1) the absorbent cotton needs to be fixed on the inner sides of 4 side faces of the adult rearing cage, the size of the absorbent cotton is equivalent to that of the side faces of the rearing cage, the consumption of the absorbent cotton is too large, the absorbent cotton can be repeatedly used, the subsequent cleaning of the absorbent cotton with the eggs of the dichocrocis punctiferalis is quite troublesome, the eggs of the dichocrocis punctiferalis are guaranteed not to be damaged in the cleaning process, so that the hatching of offspring larvae is not influenced, and the absorbent cotton fixed on the side faces of the rearing cage needs to be taken down every 2 days to be replaced by new absorbent cotton, so that the operation is time-consuming and labor-consuming; (2) except that the sponge or the absorbent cotton placed in the culture dish in the rearing cage needs to be dipped with 5-15% of honey water, the absorbent cotton fixed on 4 sides of the rearing cage also needs to be integrally dipped with 5-15% of honey water, so that the consumption of the honey water is greatly increased, and the method is uneconomical; moreover, because the side surface of the rearing cage is continuously contacted with the absorbent cotton dipped with 5-15% of honey water for 4 weeks, bacteria and viruses are easy to breed and fly and other insects are attracted if the side surface of the rearing cage is not cleaned, the honey water is difficult to clean by a common cleaning method if the side surface of the rearing cage is cleaned, and the cost for collecting eggs and rearing the dichocrocis punctiferalis is greatly increased by virtue of a detergent; (3) after the eggs of the dichocrocis punctiferalis are collected by absorbent cotton dipped with 5-15% honey water and fixed on the side surface of the rearing cage for 4 weeks, the eggs are wrapped by gauze, then the excess honey water on the absorbent cotton is slightly cleaned by tap water, the absorbent cotton is soaked by 0.5-1.5% sodium hypochlorite solution, finally the absorbent cotton is cleaned by purified water, dried and then the gauze is removed, and the eggs of the dichocrocis punctiferalis are waited to be hatched, so that the manpower and financial resources for collecting the eggs of the dichocrocis punctiferalis are greatly increased, and the resources such as the honey water, the sodium hypochlorite, the purified water and the like are wasted in the cleaning treatment process; (4) after the eggs of the dichocrocis punctiferalis attached to the absorbent cotton are cleaned and hatched, because the absorbent cotton fibers firmly bind the larvae, the larvae are difficult to break loose from the absorbent cotton fibers and climb onto the breeding food by the self force of the larvae which are hatched initially, the larvae which are hatched initially need to be picked out of the absorbent cotton by manpower and transferred to the larva breeding food, but the larvae which are hatched initially in the absorbent cotton are manually picked, so that the soft body wall tissues of the larvae which are hatched initially are easily damaged, the death rate of the larvae is greatly increased, and time and labor are wasted (application publication No. CN 108450417).
The methods for collecting eggs of dichocrocis punctiferalis have the problems of low collection efficiency, high cost, inconvenient operation and the like, and an economic method capable of simply and efficiently collecting the eggs of dichocrocis punctiferalis is urgently needed in order to better research the biology, the migration mechanism and the like of dichocrocis punctiferalis pests so as to better establish a control strategy for the dichocrocis punctiferalis. According to the method, the eggs of the dichocrocis punctiferalis can be rapidly and simply collected in a large number by using the host plant odor preferred by the dichocrocis punctiferalis, only the oviposition medium needs to be replaced for 3-4 times in the whole adult period, the replaced oviposition medium does not need to be treated, the oviposition medium is directly placed in the insect breeding box, and the larvae directly enter the corn to eat after being hatched, so that the survival rate of the larvae is increased, the cost is saved, and the collection steps of the eggs of the dichocrocis punctiferalis are simplified. In addition, the dichocrocis punctiferalis lays eggs on the gauze outside the corn cobs, so that the egg amount can be counted conveniently, and the collected eggs can be used for continuing other tests.
Disclosure of Invention
In order to solve the problems in the prior art, the invention aims to provide a simple method for quickly collecting dichocrocis punctiferalis eggs. The method can quickly, simply and massively collect the eggs of the dichocrocis punctiferalis, saves the cost, ensures that the larvae directly enter the corn cobs wrapped by the gauze to eat after the eggs on the oviposition medium gauze are incubated, and is convenient for the subsequent rearing and test of the dichocrocis punctiferalis.
The technical scheme of the invention is realized as follows:
a simple method for rapidly collecting dichocrocis punctiferalis eggs comprises the following steps:
(1) putting a proper amount of adult dichocrocis punctiferalis (50-150 heads) into a plastic spawning cage (the diameter is 25-32cm, the height is 23-30 cm), the male-female ratio is 1:1-1.5, placing a plastic culture dish (the diameter is 6-9 cm) at the bottom of the spawning cage, putting absorbent cotton dipped with sucrose solution (the mass concentration is 8% -10%) into the dish, adding the sucrose solution every day for supplementing nutrition to the adult, covering the mouth of the spawning cage with absorbent cotton gauze, and tightly sealing the mouth of the spawning cage by using a fixing ring;
(2) after the adult dichocrocis punctiferalis supplements nutrition for 1-3 days and copulates, putting a corn cob wrapped by wet absorbent cotton gauze into a spawning cage to serve as a dichocrocis punctiferalis spawning medium;
the spawning medium is that the bracts of fresh corn ears are stripped, only the corn cobs at the inner side are kept, then the corn cobs are cut into two uniform blocks along the central axis, each block is respectively wrapped by absorbent cotton gauze, the two ends of the wrapped corn are bound by white cotton threads (figure (1), transparent adhesive tapes are fixed at the two symmetrical sides of a spawning cage (figure 2), then the gauze on the surface of the corn cobs is sprayed with distilled water, and the humidity is preferably not dropped downwards;
(3) and after the dichocrocis punctiferalis produces full eggs on the egg laying medium, taking the egg laying medium out of the egg laying cage, and collecting dichocrocis punctiferalis eggs.
Typically, egg-bearing egg laying media are removed every 2-4 days and new egg laying media are placed, stopping as needed for the test or until the adult insect is no longer laying eggs.
Laying medium with eggs is placed in insect breeding boxes (25-35 cm in length, 15-20cm in width, and 15-17cm in height), 2 boxes are sprayed with a small amount of distilled water every day for moisturizing, and the larvae are incubated.
The egg laying cage (containing the imago of dichocrocis punctiferalis) and the insect breeding box (containing the ovum or larva of dichocrocis punctiferalis) are both placed in an insect breeding chamber with the temperature of 25-28 ℃, the relative humidity of 70-80% and the photoperiod of 14: 10 (L: D) -16: 8 (L: D).
The plastic spawning cages are cylindrical, the diameter is 25-32cm, the height is 23-30cm, 50-150 heads of the borer adults and 2-4 spawning media are placed in each spawning cage.
The invention has the beneficial effects that: the oviposition medium can quickly, simply and efficiently collect a large number of dichocrocis punctiferalis eggs, so that the problem of low collecting efficiency of the dichocrocis punctiferalis eggs in the past is solved, the oviposition medium can be directly used for larva feeding after collecting the dichocrocis punctiferalis eggs, the oviposition of adults and the larva feeding are effectively combined, and the problem that the larvae are artificially transferred to host plants in a time-consuming and labor-consuming manner when hatching from the eggs in the past is solved. The method can quickly, simply and efficiently collect the eggs of the dichocrocis punctiferalis, saves time and labor, is convenient for counting the eggs falling on the outer gauze of the corn cob, and is also convenient for using the collected eggs to continue other tests.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a wrapped oviposition medium of dichocrocis punctiferalis.
Fig. 2 shows the hanging mode of the dichocrocis punctiferalis oviposition medium in the insect rearing cage.
FIG. 3 is a graph comparing the effect of collecting eggs of dichocrocis punctiferalis every day in different ways.
Detailed Description
The technical solutions of the present invention will be described clearly and completely below with reference to embodiments of the present invention, and it should be apparent that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without inventive effort based on the embodiments of the present invention, are within the scope of the present invention.
Putting 60 adult dichocrocis punctiferalis in a plastic spawning cage (the diameter is 25 cm, the height is 25 cm), wherein the male-female ratio is 1:1, putting a plastic culture dish (the diameter is 6 cm) at the bottom of the spawning cage, putting absorbent cotton dipped with cane sugar water (the mass concentration is 8%) in the dish, adding the cane sugar water every day for supplementing nutrition to the adult, covering the opening of the spawning cage with absorbent cotton gauze, and sealing the opening of the spawning cage tightly (by using a fixing ring);
and after the adult dichocrocis punctiferalis supplements nutrition for 2 days and copulates, putting different oviposition media into the oviposition cage, and performing a collecting test of dichocrocis punctiferalis eggs.
Test groups: peeling off bracts outside the fresh corn ear, only keeping the corn cobs at the inner side, then cutting the corn cobs into two uniform blocks along the central axis, respectively wrapping each block with absorbent cotton gauze, binding the two ends of the wrapped corn with white cotton threads (figure 1), and fixing the corn cobs at the two symmetrical sides of the spawning cage with transparent adhesive tapes (figure 2); then, spraying distilled water on gauze on the surface of the corn cob, wherein the humidity is preferably not dropped downwards, and the gauze is used as a spawning medium of the dichocrocis punctiferalis; 2 oviposition media are put into each oviposition cage, and are replaced once every 3 days until all the female moths of the dichocrocis punctiferalis die; laying medium with eggs is placed in a pest breeding box, and a small amount of distilled water is sprayed every day to keep moisture until the larvae are hatched.
Control group 1: taking a fresh corn cob cut open from the middle as a dichocrocis punctiferalis oviposition medium, supplementing nutrition for adults for 1 day, mating, putting the cut corn cob into an oviposition cage, and replacing a new corn cob every 3 days to collect dichocrocis punctiferalis eggs.
Control group 2: moist absorbent cotton gauze is hung on the two symmetrical sides of a spawning cage to serve as a spawning medium of the dichocrocis punctiferalis, adult insects supplement nutrition for 1 day and mate, the gauze is placed into the spawning cage, and the dichocrocis punctiferalis eggs are collected by replacing new gauze every 3 days.
And recording the egg quantity of the dichocrocis punctiferalis collected by the oviposition media of the test group and the control group, thereby evaluating the effect of collecting dichocrocis punctiferalis eggs by different oviposition media.
The spawning cage (containing the imago of dichocrocis punctiferalis) or the spawning medium with eggs is placed in an insect breeding chamber with the temperature of 27 +/-1 ℃, the relative humidity of 75 +/-5% and the photoperiod of 14: 10 (L: D).
The results are shown in FIG. 3 (note: all data were analyzed for one-way anova in SPSS software, and then compared for differences between treatments using LSD's test).
Figure 3 the results show: during the whole experiment, the amount of eggs of dichocrocis punctiferalis collected by using corn and cotton gauze as oviposition media is obviously higher than that of eggs dropped on the cotton gauze or the corn cob alone (fig. 3A); the cumulative egg laying amount per female for the corn plus cotton gauze group was 343.85, which was significantly higher than 62.28 for the corn group and 64.37 for the cotton gauze group, and the difference in egg amount collected between the cotton gauze group and the corn cob group alone was not significant (fig. 3B).
Therefore, the corn cob wrapped with the cotton gauze shows a good effect of collecting eggs of dichocrocis punctiferalis, and eggs laid on the cotton gauze outside the corn cob can be used for further tests besides convenient statistics and counting.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (3)
1. A simple method for rapidly collecting dichocrocis punctiferalis eggs is characterized by comprising the following steps:
(1) putting a proper amount of adult dichocrocis punctiferalis into a spawning cage, wherein the ratio of male to female is 1:1-1.5, putting a culture dish at the bottom of the spawning cage, putting absorbent cotton dipped with cane sugar water into the culture dish, and then covering and sealing the mouth of the spawning cage by absorbent cotton gauze; the oviposition cages are cylindrical, the diameter is 25-32cm, the height is 23-30cm, and 50-150 heads of the borer moths imagoes are placed into each oviposition cage;
(2) after the egg-laying cages in the step (1) are cultivated for 1-3 days, egg-laying media are put into the egg-laying cages, then distilled water is sprayed every day, the egg-laying media are replaced periodically, and the eggs are preserved and cultivated until all adult peach borers die; in the step (2), the spawning medium is fresh corn ears, bracts are stripped from the outside, only corn cobs on the inner side are reserved, then the corn cobs are cut into two uniform blocks along the central axis, each block is respectively wrapped by absorbent cotton gauze, and the corn cobs are sprayed with distilled water to be wet, and the humidity is preferably not dropped downwards; the period of replacing the oviposition medium regularly in the step (2) is 2-4 days, and distilled water is sprayed every day to ensure that the cultivation humidity is 70-80%;
(3) and (3) taking out the oviposition medium treated in the step (2) from the oviposition cage, and collecting the dichocrocis punctiferalis eggs.
2. The simple method for rapidly collecting dichocrocis punctiferalis eggs according to claim 1, which is characterized in that: the mass concentration of the sucrose water in the step (1) is 8-10%.
3. The easy method for rapidly collecting dichocrocis punctiferalis eggs according to claim 1, wherein the spawning cage culture conditions in the step (2) are as follows: placing in an insect-raising room with temperature of 25-28 deg.C, relative humidity of 70-80% and photoperiod of 14: 10 (L: D) -16: 8 (L: D).
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105265394A (en) * | 2015-10-26 | 2016-01-27 | 西南大学 | Red illumination method for prompting mating and oviposition of artificially bred dichocrocis punctiferalis |
| CN108142382A (en) * | 2018-02-01 | 2018-06-12 | 河北省农林科学院植物保护研究所 | A kind of method of large-scale breeding dichocrocis punctiferalis |
| CN108450417A (en) * | 2018-02-01 | 2018-08-28 | 河北省农林科学院植物保护研究所 | Application of the absorbent cotton on dichocrocis punctiferalis ovum collecting |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105265394A (en) * | 2015-10-26 | 2016-01-27 | 西南大学 | Red illumination method for prompting mating and oviposition of artificially bred dichocrocis punctiferalis |
| CN108142382A (en) * | 2018-02-01 | 2018-06-12 | 河北省农林科学院植物保护研究所 | A kind of method of large-scale breeding dichocrocis punctiferalis |
| CN108450417A (en) * | 2018-02-01 | 2018-08-28 | 河北省农林科学院植物保护研究所 | Application of the absorbent cotton on dichocrocis punctiferalis ovum collecting |
Non-Patent Citations (1)
| Title |
|---|
| 桃蛀螟人工饲养滞育诱导特性及抗寒性研究;徐丽荣;《中国优秀硕士学位论文全文数据库 农业科技辑》;20111015;第7-12页 * |
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