CN107711723A - A kind of maize aphidses method for breeding for being suitable to the cecidomyiia breeding of food aphid and its application - Google Patents
A kind of maize aphidses method for breeding for being suitable to the cecidomyiia breeding of food aphid and its application Download PDFInfo
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- CN107711723A CN107711723A CN201711079025.5A CN201711079025A CN107711723A CN 107711723 A CN107711723 A CN 107711723A CN 201711079025 A CN201711079025 A CN 201711079025A CN 107711723 A CN107711723 A CN 107711723A
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- aphid
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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Abstract
The invention belongs to insect artificial propagation techniques field, and in particular to and it is a kind of to be suitable to eat the maize aphidses method for breeding that aphid cecidomyiia breeds, and using this method eat the anniversary artificial fecundation method of aphid cecidomyiia.The maize aphidses method for breeding of the present invention for being suitable to the cecidomyiia breeding of food aphid, by controlling the parameters in aphid growth course, controls photoperiod L13 16：D8 11, the 4000lx of intensity of illumination 2000 so that larvae aphid survival rate height, adult aphid life-span and the breeding period length bred, its average reproductive capacity are higher.
Description
Technical field
The invention belongs to insect artificial propagation techniques field, and in particular to a kind of maize aphidses for being suitable to the cecidomyiia breeding of food aphid
Method for breeding, and using this method carry out eat aphid cecidomyiia anniversary artificial fecundation method.
Background technology
Aphid cecidomyiia Aphidoletes aphidimyza (Rondani) are eaten, for Diptera, Cecidomyiidae insect, its host elder brother
Worm is mainly cotten aphid, and the crop such as main harm cotton, and in China, food aphid cecidomyiia is distributed mainly on the areas such as Hubei.Eat aphid cecidomyiia
It is to be survived the winter with the larva that cocoons under table soil around the host plant of aphid, pupates, emergence post-coitum, having between the month in next year 3-4
The upper egg reproduction of the early spring such as weeds and the rose of Sharon of aphid host.Annual April is upper, the middle ten days is first generation Adult worms producting eggs peak period, May
The upper, the middle ten days is second generation Adult worms producting eggs peak period, and in 5 months, the last ten-days period can see second generation carnivorism cecidomyiia larva predation cotton in cotton field
Aphid, but at this moment have generation quantity on aphid plant more outside field.Eat the adult Quick off the mark of aphid cecidomyiia and circle in the air rapid and more
Laid eggs on the vacuum side of blade or tender stem for have aphid, often place's spawning general several, up to more than 10 grains.It can be preyed on after larvae hatch
Nascent larvae aphid, adult aphid is preyed on after growing up, belly or foot for hooking aphid with oral hook etc., draw body fluid；After larva is aging, enter
Soil, which is cocoond, is pupated, and population quantity can be reduced typically after June；It is especially quick to chemical pesticide and its adult is due in delicate health
Sense, and the ability of its high temperature resistance, drying and wind and rain is also weaker.
Food aphid cecidomyiia is the important predator of aphid, the aphid in more than 60 kind Different Crops can be preyed on, wherein wrapping
Many important agricultural pests are included, such as black peach aphid, repair tail aphid, cotten aphid, bean aphid, radish aphid, brevicoryne brassicae, spiraea aphid, are control aphids
The effective natural enemies of worm harm.Eat aphid cecidomyiia it is larger to aphid lethality, every larva not only throughout one's life can the bull of feeding aphid 50, and
And when aphid density is high, it is more that lethal aphid is inhaled by thorn.Early in 1916, Davis just pointed out that it is aphid to eat aphid cecidomyiia
A kind of biological and ecological methods to prevent plant disease, pests, and erosion controlling elements with very big potential value.
In China, just there is the report of food aphid cecidomyiia distribution early in the forties, but report seldom on the research of this worm.It is existing
Have been reported that it is host, using barley seedling as vector plant to use using corn leaf aphids Rhopalosiphum maidis (Filch) in technology
Mode expand it is numerous food aphid cecidomyiia.However, because aphid growth is limited and the parasitic effects of aphid chalcid fly by external environmental condition, and
Aphid is more sensitive to light application ratio, and illumination not only influences spatial and temporal distributions, Sex Differentiation, wing form determination and body-color diversity of aphid etc.,
And Development and reproduction to aphid etc. can be had an impact so that the scale of corn leaf aphids is difficult in actual production
Change annual to breed, the yield for causing to eat aphid cecidomyiia is also greatly affected.
The content of the invention
Therefore, the technical problems to be solved by the invention are that providing a kind of maize aphidses for being suitable to the cecidomyiia breeding of food aphid raises
The method of supporting, to solve the problems, such as that the maize aphidses anniversary amount of breeding is relatively low in the prior art.
The invention also discloses a kind of anniversary artificial fecundation method for eating aphid cecidomyiia.
A kind of in order to solve the above technical problems, maize aphidses raising side for being suitable to the cecidomyiia breeding of food aphid of the present invention
Method, comprise the following steps：
(1) barley seedling is planted：Barley is conventionally planted in glasshouse, after barley emerges, in barley seedling
Upper inoculation aphid, it is standby；
(2) aphid is connect：The barley seedling after aphid will be inoculated with and move into climatic chamber, mount silk screen, and by the barley leaf with aphid
Piece is put on silk screen；
(3) 24-26 DEG C, relative humidity 60%-70%, photoperiod L13-16 of weather indoor temperature is controlled：D8-11, illumination
Intensity 2000-4000lx, carry out breeding for aphid.
In the step (1), the inoculation aphid step is carried out for 2-3 days after barley emerges.
It is after the barley leaves with aphid are put on silk screen, in addition to after one day that old blade is clear in the step (2)
Except the step of.
In the step (3), in addition to after aphid grow 7-8 days after be inoculated with aphid again the step of.
In the step (3), it is additionally included in climatic chamber and places the step of growth promotes liquid.
It is the sorbitol solution that mass concentration is 5-10wt% that the growth, which promotes liquid,.
The growth promotes liquid also to include the serine solution that mass concentration is 1-2wt%.
The invention also discloses the described maize aphidses method for breeding for being suitable to the cecidomyiia breeding of food aphid in the food aphid cecidomyiia anniversary
The application in artificial propagation field.
Enter the invention also discloses a kind of anniversary artificial fecundation method for eating aphid cecidomyiia, including according to described method for breeding
The step of row maize aphidses are bred, and the step of inoculation food aphid cecidomyiia.
In the inoculation step, the benefit evil of food aphid cecidomyiia is than being 1：60-80.
The maize aphidses method for breeding of the present invention for being suitable to the cecidomyiia breeding of food aphid, by controlling in aphid growth course
Parameters, control photoperiod L13-16：D8-11, intensity of illumination 2000-4000lx so that the larvae aphid survival rate bred is high, into
Aphid life-span and breeding period length, its average reproductive capacity are higher；The present invention it is more excellent breed in workshop set by D-sorbite and silk
The growth of propylhomoserin composition promotes liquid, and to promote the growth of aphid, the culture and anniversary for being more beneficial for aphid are bred.Beneficial to the present invention
The maize aphidses method for breeding, which can be bred, obtains substantial amounts of high-grade maize aphid, has been provided for breeding in anniversary for aphid cecidomyiia of food
The condition of profit.
Embodiment
Embodiment 1
The maize aphidses method for breeding for being suitable to the cecidomyiia breeding of food aphid described in the present embodiment, comprises the following steps：
(1) barley seedling is planted：Barley is conventionally planted in glasshouse, after barley emerges 2 days, in barley
Aphid larva is inoculated with seedling, it is standby；
(2) aphid is connect：The barley seedling after aphid will be inoculated with and move into climatic chamber, mount silk screen, and by the barley leaf with aphid
Piece is put on silk screen, and is removed old blade after Yu Yitian；
(3) 24-26 DEG C, relative humidity 60%-70%, photoperiod L13 of weather indoor temperature is controlled：D11, intensity of illumination
3000lx, breeding for aphid is carried out, while aphid larva is inoculated with again after aphid grows 7-8 days, treat that aphid density reaches every
During 20, blade, available for food aphid cecidomyiia inoculation and breed.
Embodiment 2
The maize aphidses method for breeding for being suitable to the cecidomyiia breeding of food aphid described in the present embodiment, comprises the following steps：
(1) barley seedling is planted：Barley is conventionally planted in glasshouse, after barley emerges 3 days, in barley
Aphid larva is inoculated with seedling, it is standby；
(2) aphid is connect：The barley seedling after aphid will be inoculated with and move into climatic chamber, mount silk screen, and by the barley leaf with aphid
Piece is put on silk screen, and is removed old blade after Yu Yitian；
(3) 24-26 DEG C, relative humidity 60%-70%, photoperiod L14 of weather indoor temperature is controlled：D10, intensity of illumination
2000lx, breeding for aphid is carried out, while aphid larva is inoculated with again after aphid grows 7-8 days, treat that aphid density reaches every
During 20, blade, available for food aphid cecidomyiia inoculation and breed.
Embodiment 3
The maize aphidses method for breeding for being suitable to the cecidomyiia breeding of food aphid described in the present embodiment, comprises the following steps：
(1) barley seedling is planted：Barley is conventionally planted in glasshouse, after barley emerges 3 days, in barley
Aphid larva is inoculated with seedling, it is standby；
(2) aphid is connect：The barley seedling after aphid will be inoculated with and move into climatic chamber, mount silk screen, and by the barley leaf with aphid
Piece is put on silk screen, and is removed old blade after Yu Yitian；
(3) 24-26 DEG C, relative humidity 60%-70%, photoperiod L15 of weather indoor temperature is controlled：D9, intensity of illumination
4000lx, breeding for aphid is carried out, while aphid larva is inoculated with again after aphid grows 7-8 days, treat that aphid density reaches every
During 20, blade, available for food aphid cecidomyiia inoculation and breed.
Embodiment 4
The maize aphidses method for breeding for being suitable to the cecidomyiia breeding of food aphid described in the present embodiment, comprises the following steps：
(1) barley seedling is planted：Barley is conventionally planted in glasshouse, after barley emerges 3 days, in barley
Aphid larva is inoculated with seedling, it is standby；
(2) aphid is connect：The barley seedling after aphid will be inoculated with and move into climatic chamber, mount silk screen, and by the barley leaf with aphid
Piece is put on silk screen, and is removed old blade after Yu Yitian；
(3) 24-26 DEG C, relative humidity 60%-70%, photoperiod L16 of weather indoor temperature is controlled：D8, intensity of illumination
3000lx, breeding for aphid is carried out, while aphid larva is inoculated with again after aphid grows 7-8 days, treat that aphid density reaches every
During 20, blade, available for food aphid cecidomyiia inoculation and breed.
Embodiment 5
The maize aphidses method for breeding for being suitable to the cecidomyiia breeding of food aphid described in the present embodiment only exists with embodiment 1, its difference
In in the step (3), being additionally included in climatic chamber and place the step of growth promotes liquid, it is that quality is dense that the growth, which promotes liquid,
Spend the sorbitol solution for 5wt%.
Embodiment 6
The maize aphidses method for breeding for being suitable to the cecidomyiia breeding of food aphid described in the present embodiment only exists with embodiment 1, its difference
In in the step (3), being additionally included in climatic chamber and place the step of growth promotes liquid, it is that quality is dense that the growth, which promotes liquid,
Spend the sorbitol solution for 10wt%.
Embodiment 7
The maize aphidses method for breeding for being suitable to the cecidomyiia breeding of food aphid described in the present embodiment only exists with embodiment 6, its difference
In the growth promotes liquid also to include the serine solution that mass concentration is 1wt%.
Embodiment 8
The maize aphidses method for breeding for being suitable to the cecidomyiia breeding of food aphid described in the present embodiment only exists with embodiment 6, its difference
In the growth promotes liquid also to include the serine solution that mass concentration is 2wt%.
Comparative example 1
The maize aphidses method for breeding for being suitable to the cecidomyiia breeding of food aphid described in this comparative example only exists with embodiment 1, its difference
In in the step (3), the photoperiod is L10：D14.
Comparative example 2
The maize aphidses method for breeding for being suitable to the cecidomyiia breeding of food aphid described in this comparative example only exists with embodiment 1, its difference
In in the step (3), it is 4500-7000lx to control intensity of illumination.
Comparative example 3
The maize aphidses method for breeding for being suitable to the cecidomyiia breeding of food aphid described in this comparative example only exists with embodiment 1, its difference
In, in the step (3), control intensity of illumination 1200-1800lx.
Comparative example 4
The maize aphidses method for breeding for being suitable to the cecidomyiia breeding of food aphid described in this comparative example only exists with embodiment 1, its difference
In in the step (2), after the barley leaves with aphid are put on silk screen, old blade is removed after 2 days；It is and described
In step (3), the photoperiod is L10：D14, and aphid is inoculated with again after aphid grows 10 days.
Experimental example
In a diameter of 9 ㎝ culture dish, one layer of upper berth filter paper, 3-5cm sizes are taken to be laid in filter fresh barley leaves
On paper, vacuum side of blade is upward, is changed 1 time per 3d, and injection proper amount of clear water ensures that blade is fresh in ware.If connect 1 primiparity per ware
Aphid, observed 1 time per 24h, aphid survival condition is recorded, until larvae aphid grows to adult aphid.During adult aphid, record adult aphid production aphid quantity
And survival condition, while the larvae aphid produced is removed, each examination aphid quantity that handles is 70.Record above-described embodiment 1-8
And larvae aphid survival rate, adult aphid life-span and fecundity the results are shown in Table 1 in comparative example 1-4.
Influence of the photoperiod of table 1 to corn leaf aphids adult aphid life-span and fecundity
Above-mentioned result of the test shows, in the method for the invention, photoperiod L13-16 is controlled in aphid growth course：D8-
When 11, intensity of illumination 2000-4000lx, larvae aphid survival rate is high, adult aphid life-span and breeding period length, average reproductive capacity are high；And connecing
Aphid removed old blade after one day, was inoculated with aphid again after aphid grows 7-8 days, larvae aphid survival rate is significantly higher；Especially
It is to grow promotion liquid breeding to set in workshop to promote the growth of aphid, the anniversary for being advantageous to aphid breeds.
Application examples
Method according to above-described embodiment 1-8 carries out maize aphidses and bred, take after inoculation growth 2-3 days and breeding have it is big
After the wheat seeding basin of amount aphid moves into food aphid cecidomyiia workshop, 800 food aphid cecidomyiia adults, the spawning of male and female post-coitum, control are put
Workshop temperature is maintained at 21-28 DEG C, and relative humidity is maintained at 60-75%.When breeding by the 5-7 days, aphid cecidomyiia larva is eaten in part
Oneself is reached maturity, and potted plant barley is inverted in above the water pond for filling clear water, and mature larva is snapped into water automatically, daily timing
Mature larva in water pond is taken out and removes the impurity such as aphid corpse.The mature larva being collected into is loaded simultaneously and contains vermiculite
In capsule, keep vermiculite moist, mature larva is pupated in vermiculite, can preserved 1 month under the conditions of 13 DEG C in insulating box, can
Realize breeding for food aphid cecidomyiia.
Obviously, above-described embodiment is only intended to clearly illustrate example, and is not the restriction to embodiment.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of change or
Change.There is no necessity and possibility to exhaust all the enbodiments.And the obvious change thus extended out or
Among changing still in the protection domain of the invention.
Claims (10)
1. a kind of maize aphidses method for breeding for being suitable to the cecidomyiia breeding of food aphid, it is characterised in that comprise the following steps：
(1) barley seedling is planted：Barley is conventionally planted in glasshouse, after barley emerges, in being connect on barley seedling
Kind aphid, it is standby；
(2) aphid is connect：Barley seedling after inoculation aphid is moved into climatic chamber, mounts silk screen, and the barley leaves with aphid are put
Onto silk screen；
(3) 24-26 DEG C, relative humidity 60%-70%, photoperiod L13-16 of weather indoor temperature is controlled：D8-11, intensity of illumination
2000-4000lx, carry out breeding for aphid.
2. the maize aphidses method for breeding according to claim 1 for being suitable to the cecidomyiia breeding of food aphid, it is characterised in that the step
Suddenly in (1), the inoculation aphid step is carried out for 2-3 days after barley emerges.
3. the maize aphidses method for breeding according to claim 1 or 2 for being suitable to the cecidomyiia breeding of food aphid, it is characterised in that institute
State in step (2), after the barley leaves with aphid are put on silk screen, in addition to the step of old blade is removed after one day.
4. the maize aphidses method for breeding for being suitable to the cecidomyiia breeding of food aphid according to claim any one of 1-3, its feature exist
In, in the step (3), in addition to after aphid grow 7-8 days after be inoculated with aphid again the step of.
5. the maize aphidses method for breeding for being suitable to the cecidomyiia breeding of food aphid according to claim any one of 1-4, its feature exist
In in the step (3), being additionally included in climatic chamber and place the step of growth promotes liquid.
6. the maize aphidses method for breeding according to claim 5 for being suitable to the cecidomyiia breeding of food aphid, it is characterised in that the life
Long promotion liquid is the sorbitol solution that mass concentration is 5-10wt%.
7. the maize aphidses method for breeding according to claim 6 for being suitable to the cecidomyiia breeding of food aphid, it is characterised in that the life
It is long to promote liquid also to include the serine that mass concentration is 1-2wt%.
8. the maize aphidses method for breeding for being suitable to the cecidomyiia breeding of food aphid described in claim any one of 1-7 is in the food aphid cecidomyiia anniversary
The application in artificial propagation field.
9. a kind of anniversary artificial fecundation method for eating aphid cecidomyiia, it is characterised in that including according to described in claim any one of 1-7
Method for breeding carry out maize aphidses the step of breeding, and the step of inoculation food aphid cecidomyiia.
10. the anniversary artificial fecundation method of food aphid cecidomyiia according to claim 9, it is characterised in that the inoculation step
In, the benefit evil of food aphid cecidomyiia is than being 1：60-80.
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| CN201711079025.5A CN107711723A (en) | 2017-11-06 | 2017-11-06 | A kind of maize aphidses method for breeding for being suitable to the cecidomyiia breeding of food aphid and its application |
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| CN201711079025.5A CN107711723A (en) | 2017-11-06 | 2017-11-06 | A kind of maize aphidses method for breeding for being suitable to the cecidomyiia breeding of food aphid and its application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109601483A (en) * | 2018-12-11 | 2019-04-12 | 安阳工学院 | A kind of method for breeding making corn leaf aphids life table |
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