CN102835596A - Artificial feed of proxenus lepigone larva - Google Patents
Artificial feed of proxenus lepigone larva Download PDFInfo
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- CN102835596A CN102835596A CN2012103139816A CN201210313981A CN102835596A CN 102835596 A CN102835596 A CN 102835596A CN 2012103139816 A CN2012103139816 A CN 2012103139816A CN 201210313981 A CN201210313981 A CN 201210313981A CN 102835596 A CN102835596 A CN 102835596A
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Abstract
The invention discloses an artificial feed of proxenus lepigone larva. According to part by weight, the feed comprises the following constituents of 200-220 parts of corn starch, 118-125 parts of soybean meal, 40-43 parts of yeast powder, 27-29 parts of sugar, 3.5-4 parts of sorbic acid, 28-30 parts of agar, 40-42 parts of casein, 10-12 parts of composite vitamin, 2-3.5 parts of vitamin C, 0.1-0.2 part of streptomycin sulfate, 2.5-5 parts of methanol, 4-6 parts of alcohol, 1-3 parts of vegetable oil and 1000-1500 parts of sterilized distilled water. The artificial feed of proxenus lepigone larva, disclosed by the invention, is available in raw material and low in cost, is convenient to prepare, can be stored for long time, has comprehensive nutritional ingredient, stable feed effect, high practicability and obvious economical benefit, and is favorable for raising the proxenus lepigone larva on a large scale.
Description
Technical field
The present invention relates to the man-made feeds of a kind of insect, particularly relate to a kind of man-made feeds of 2 committees of insect exigua larvae that happens suddenly.
Background technology
2 committee noctuids
Proxenus lepigone(Moschler), different name
Athetis lepigone, belong to Lepidoptera, Noctuidae, committee's Noctua is the de novo important pests of corn field after China's cropping system (no-tillage and subsides stubble sowing after the wheat harvest) is changed, damaging range enlarges year by year.2 committee noctuids endangered in seedling stage, mainly sting food corn basal part of stem and with newborn aerial root, mainly gnaw basal part of stem in the corn 2-6 leaf phase, form the cavity, cause the aerial part lobus cardiacus to wilt withered or cause the plant lodging.Larva changes strain along ridge harm, gnaws young tender watermelon skin zone 6-7 month, makes it can not grow up or cause the germ invasion and attack, and larva is liked dark wet environment, the fear high light, and the broken wheat straw of often hiding around corn seedling endangers down.In the corn producing region, in case a large amount of the generation often caused the disconnected ridge of being short of seedling, even large stretch of dead seedling appears.Because of be difficult to find larva in the field, the control difficulty utilizes other this worm of method control to become the focus of current control.
Use man-made feeds to raise insect, the history in year surplus in the of existing 100.At insect physiology, toxicity, pathology, steriliation by irradiation, chemical sterilant, research such as attractant and pheromone is gone up and is needed to use a large amount of insects, raises insect with man-made feeds, just becomes an important process.2 committee noctuid adults are active, and larva is difficult in the field find, causes the artificial feeding difficulty; And not having ready-made method can reference; To these problems,, not only test material is provided for biological toxicity test for 2 committees of a large amount of breedings noctuid; For the exploitation of microorganism biological agricultural chemicals provides basis, worm source, we have carried out invention research to the man-made feeds of 2 committee's exigua larvaes simultaneously.
Document number is the man-made feeds that the file of CN102578432A discloses a kind of 2 committee's exigua larvaes, in this feed Amaranthus retroflexus leaf powder is used for 2 committee's exigua larvae feeds, and as primary raw material; And Amaranthus retroflexus is a weeds in field; Only be distributed in the few some areas of China, and receive seasonal restriction, be difficult to obtain in most of areas or time; Cause difficulty for 2 committees of raising exigua larvaes, be not easy to implement; In addition, the normal enrichment nitrate of this plant, larva is excessive possibly to cause poisoning after edible.
Summary of the invention
The technical problem that the present invention solved provides that a kind of raw material are easy to get, comprehensive nutrition and 2 high committee's exigua larvae man-made feeds of survival rate.
Technical scheme of the present invention:
A kind of 2 committee's exigua larvae man-made feeds is characterized in that, represent with weight portion; Contain 220 portions of corn flour of following component: 200-in the said feed, 118-125 part analysis for soybean powder, 40-43 part dusty yeast; 27-29 part sucrose, 3.5-4 part sorbic acid, 28-30 part agar; 40-42 part casein, 10-12 part B B-complex; 2-3.5 part vitamin C; 0.1-0.2 portion streptomycin sulphate, 2.5-5 part formaldehyde, 4-6 part alcohol, 1-3 part vegetable oil, 1000-1500 part sterile purified water.
Preferably contain 215 portions of corn flour of following component: 205-in the said feed, 120-123 part analysis for soybean powder, 41-42 part dusty yeast, 28-29 part sucrose, 3.5-4 part sorbic acid, 29-30 part agar, 41-42 part casein, 11-12 part B B-complex; 3-3.5 part vitamin C; 0.1-0.2 portion streptomycin sulphate, 3-4 part formaldehyde, 4-5 part alcohol, 1-2 part vegetable oil, 1000-1500 part sterile purified water.
Said B B-complex is formulated by following vitamin: thiamine hydrochloride 0.6-0.7 part, inositol 5-7 part, folic acid 0.3-0.5 part, Choline Chloride 10-14 part, tocopherol 1-3 part; Described edible vegetable oil is peanut oil or soybean oil.
The man-made feeds of said 2 committee's exigua larvaes, its preparation method comprise that raw material grinds even, mixes, stirs, and it is characterized in that, specifically may further comprise the steps:
1) component A: corn flour, analysis for soybean powder and dusty yeast are ground even, mix, make component A;
2) B component: 400-600 part distilled water is heated to boiling, adds sorbic acid and stir, again solution is poured into thin rod stirs among the component A, make B component;
3) component C: in 500-700 part distilled water, add agar and be heated to and dissolve, add casein again and be stirred to and dissolve, mixed liquor is poured in the B component stirred, make component C;
4) get B B-complex, vitamin C, streptomycin sulphate, 100-200 part distilled water, stir, when treating that component C is cooled to 38 ~ 42 ℃; Pour formaldehyde, alcohol and vegetable oil into, stir, cool to room temperature; Be 2 committee's exigua larvae man-made feeds, put into 4 ℃ of refrigerators and preserve.
It is glass bar or dactylethrae that said raw material grinds even instrument, and said stirring tool is aseptic thin glass bar or aseptic thin stick.
The method for breeding of said man-made feeds:
2 committee's exigua larvae man-made feeds are cut into the bulk of suitable size; Pack into the sterilization foster scolite in; On the access feed that 2 committee's exigua larvaes of just hatching are careful; Sealing the mouth of pipe with tampon or preservative film, it is 26 ± 0.5oC that larva is placed temperature, and humidity is complete dark raising the in 80% the incubator.
Positive beneficial effect of the present invention:
1) the artificial feed making method of the present invention is easy, and a technician can independently accomplish feed preparation work through simple training under no any special installation.
2) the artificial feed of the present invention has overcome the defective that the main raw material(s) Amaranthus retroflexus is not easy to obtain among the file CN102578432A, and raw material are easy to get, and are cheap, and is nutritious comprehensive, has no side effect remarkable in economical benefits.
3) the present invention adds feed with streptomycin sulphate, has improved the ability of the disease-resistant bacterium of larva greatly, has reduced the death rate of larva to a great extent; Use the antiseptic effect that has improved feed in the time of alcohol and formaldehyde, make the feed resting period long for a long time; Edible vegetable oil contains nutritional labelings such as unrighted acids such as linoleic acid and vitamin E, can be absorbed by the insect organism well.
4) man-made feeds of the present invention are raised 2 committee's exigua larvaes, raise for bionical attitude, and the larva survival rate is high, and the history of life, adult spawning rate and hundred ovum were heavy obviously greater than raising with maize seedling merely than shortening 2-3 days with simple the raising with maize seedling.
5) the present invention can raise 2 committee noctuids by subculture, has very strong practicality, and development duration is neat, and feeding effect is stable, for the toxicity test experiment provides the length of time consistent worm source with the exploitation microbial pesticide.
The specific embodiment:
To further explain of the present invention, but the invention is not restricted to these embodiment below in conjunction with specific embodiment, in following examples raw materials used be commercially available.
Instance 1:
A kind of 2 committee's exigua larvae man-made feeds, in weight portion, this feed is processed by following raw material: 200 portions of corn flour, 118 parts of analysis for soybean powder, 40 parts of dusty yeasts, 27 portions of sucrose, 3.5 parts of sorbic acids, 28 parts of agar, 40 parts of caseins, 10 parts of B B-complexes; 2 parts of vitamin Cs; 0.1 portion streptomycin sulphate, 2.5 parts of formaldehyde, 4 parts of alcohol, 1 portion of peanut oil, 1000 parts of sterile purified waters.
Wherein B B-complex is formulated by following vitamin: 0.6 part of thiamine hydrochloride, 5 parts of inositols, 0.3 part in folic acid, 10 parts of Choline Chlorides, 1 part of tocopherol.
The concrete configuration step of man-made feeds is:
1) component A: corn flour, analysis for soybean powder and dusty yeast are ground even, mix, make component A;
2) B component: 400 parts of distilled water → be heated to boiling → adding sorbic acid stirs → pours into, and thin rod stirs among the component A, makes B component;
3) component C:500 part distilled water → adding agar and be heated to and dissolve → add casein (be stirred to and dissolve) → pour in the B component and stir makes component C;
4) get B B-complex, vitamin C, streptomycin sulphate and 100 parts of distilled water, in triangular flask, shake up, when treating that component C is cooled to 40 ℃, pour formaldehyde, alcohol and peanut oil into, stir, cool to room temperature.
Method for breeding:
With the man-made feeds stripping and slicing diameter of packing into is 2-4cm; Length is the sterilization glass dactylethrae of 5cm or the plastic tube of crossing with alcohol wipe; Be filled to pipe 3/5-4/5 place until feed; 2 committee noctuids are placed on the feed with little writing brush except that incubating larva carefully, are sealing the mouth of pipe, place growth cabinet to raise the foster scolite that worm is housed with tampon or preservative film.Every at a distance from man-made feeds of replacing in 7-10 days, larva freely grows in pipe and gets food until pupating.
Instance 2:
A kind of 2 committee's exigua larvae man-made feeds: in weight portion, this feed is processed by following raw material: 200.1 portions of corn flour, 118.1 parts of analysis for soybean powder; 40.1 part dusty yeast, 27.1 portions of sucrose, 3.6 parts of sorbic acids; 28.1 part agar, 40.1 parts of caseins, 10.1 parts of B B-complexes; 2.1 part vitamin C; 0.11 portion streptomycin sulphate, 2.6 parts of formaldehyde, 4.1 parts of alcohol, 1.1 portions of peanut oil, 1010 parts of sterile purified waters.
Wherein B B-complex is formulated by following vitamin: 0.61 part of thiamine hydrochloride, 5.1 parts of inositols, 0.31 part in folic acid, 10.1 parts of Choline Chlorides, 1.1 parts of tocopherols.
The concrete configuration step of man-made feeds is:
1) component A: corn flour, analysis for soybean powder and dusty yeast are ground even, mix, make component A;
2) B component: 400 parts of distilled water → be heated to boiling → adding sorbic acid stirs → pours into, and thin rod stirs among the component A, makes B component;
3) component C:510 part distilled water → adding agar and be heated to and dissolve → add casein (be stirred to and dissolve) → pour in the B component and stir makes component C;
4) get B B-complex, vitamin C, streptomycin sulphate and 100 parts of distilled water, in triangular flask, shake up, when treating that component C is cooled to 40 ℃, pour formaldehyde, alcohol and peanut oil into, stir, cool to room temperature.
Concrete method for breeding is identical with embodiment 1.
Instance 3:
A kind of 2 committee's exigua larvae man-made feeds: in weight portion, this feed is processed by following raw material: 210 portions of corn flour, 121 parts of analysis for soybean powder, 41 parts of dusty yeasts, 28 portions of sucrose, 3.8 parts of sorbic acids, 29 parts of agar, 41 parts of caseins, 11 parts of B B-complexes; 3 parts of vitamin Cs; 0.15 portion streptomycin sulphate, 3.5 parts of formaldehyde, 5 parts of alcohol, 2 portions of soybean oils, 1250 parts of sterile purified waters.
Said B B-complex is formulated by following vitamin: 0.65 part of thiamine hydrochloride, 6 parts of inositols, 0.4 part in folic acid, 12 parts of Choline Chlorides, 2 parts of tocopherols.
The concrete configuration step of man-made feeds is:
1) component A: corn flour, analysis for soybean powder and dusty yeast are ground even, mix, make component A;
2) B component: 500 parts of distilled water → be heated to boiling → adding sorbic acid stirs → pours into, and thin rod stirs among the component A, makes B component;
3) component C:600 part distilled water → adding agar and be heated to and dissolve → add casein (be stirred to and dissolve) → pour in the B component and stir makes component C;
4) get B B-complex, vitamin C, streptomycin sulphate and 150 parts of distilled water, in triangular flask, shake up, when treating that component C is cooled to 40 ℃, pour formaldehyde, alcohol and soybean oil into, stir, cool to room temperature.
Concrete method for breeding is identical with embodiment 1.
Instance 4:
A kind of 2 committee's exigua larvae man-made feeds: in weight portion, this feed is processed by following raw material: 219.9 portions of corn flour, 124.9 parts of analysis for soybean powder; 42.9 part dusty yeast, 28.9 portions of sucrose, 3.9 parts of sorbic acids; 29.9 part agar, 41.9 parts of caseins, 11.9 parts of B B-complexes; 3.4 part vitamin C; 0.19 portion streptomycin sulphate, 4.9 parts of formaldehyde, 5.9 parts of alcohol, 2.9 portions of peanut oil, 1490 parts of sterile purified waters.
Wherein B B-complex is formulated by following vitamin: 0.69 part of thiamine hydrochloride, 6.9 parts of inositols, 0.49 part in folic acid, 13.9 parts of Choline Chlorides, 2.9 parts of tocopherols.
The concrete configuration step of man-made feeds is:
1) component A: corn flour, analysis for soybean powder and dusty yeast are ground even, mix, make component A;
2) B component: 600 parts of distilled water → be heated to boiling → adding sorbic acid stirs → pours into, and thin rod stirs among the component A, makes B component;
3) component C:690 part distilled water → adding agar and be heated to and dissolve → add casein (will be stirred to and dissolve) → pour in the B component and stir makes component C;
4) get B B-complex, vitamin C, streptomycin sulphate and 200 parts of distilled water, in triangular flask, shake up, when treating that component C is cooled to 40 ℃, pour formaldehyde, alcohol and peanut oil into, stir, cool to room temperature.
Concrete method for breeding is identical with embodiment 1.
Instance 5:
A kind of 2 committee's exigua larvae man-made feeds: in weight portion, this feed is processed by following raw material: 220 portions of corn flour, 125 parts of analysis for soybean powder, 43 parts of dusty yeasts, 29 portions of sucrose, 4 parts of sorbic acids, 30 parts of agar, 42 parts of caseins, 12 parts of B B-complexes; 3.5 part vitamin C; 0.2 portion streptomycin sulphate, 5 parts of formaldehyde, 6 parts of alcohol, 3 portions of peanut oil, 1500 parts of sterile purified waters.
Wherein B B-complex is formulated by following vitamin: 0.7 part of thiamine hydrochloride, 7 parts of inositols, 0.5 part in folic acid, 14 parts of Choline Chlorides, 3 parts of tocopherols.
The concrete configuration step of man-made feeds is:
1) component A: corn flour, analysis for soybean powder and dusty yeast are ground even, mix, make component A;
2) B component: 600 parts of distilled water → be heated to boiling → adding sorbic acid stirs → pours into, and thin rod stirs among the component A, makes B component;
3) component C:700 part distilled water → adding agar and be heated to and dissolve → add casein (will be stirred to and dissolve) → pour in the B component and stir makes component C;
4) get B B-complex, vitamin C, streptomycin sulphate and 200 parts of distilled water, in triangular flask, shake up, when treating that component C is cooled to 40 ℃, pour formaldehyde, alcohol and peanut oil into, stir, cool to room temperature.
Concrete method for breeding is identical with embodiment 1.
Instance 6:
A kind of 2 committee's exigua larvae man-made feeds: in weight portion, this feed is processed by following raw material: 205 portions of corn flour, 120 parts of analysis for soybean powder, 41 parts of dusty yeasts, 28 portions of sucrose, 3.5 parts of sorbic acids, 29 parts of agar, 41 parts of caseins, 11 parts of B B-complexes; 3 parts of vitamin Cs; 0.1 portion streptomycin sulphate, 4 parts of formaldehyde, 5 parts of alcohol, 1 portion of peanut oil, 1000 parts of sterile purified waters.
Wherein B B-complex is formulated by following vitamin: 0.65 part of thiamine hydrochloride, 5.5 parts of inositols, 0.35 part in folic acid, 11 parts of Choline Chlorides, 1.5 parts of tocopherols.
The concrete configuration step of man-made feeds is:
1) component A: corn flour, analysis for soybean powder and dusty yeast are ground even, mix, make component A;
2) B component: 400 parts of distilled water → be heated to boiling → adding sorbic acid stirs → pours into, and thin rod stirs among the component A, makes B component;
3) component C:500 part distilled water → adding agar and be heated to and dissolve → add casein (will be stirred to and dissolve) → pour in the B component and stir makes component C;
4) get B B-complex, vitamin C, streptomycin sulphate and 100 parts of distilled water, in triangular flask, shake up, when treating that component C is cooled to 40 ℃, pour formaldehyde, alcohol and peanut oil into, stir, cool to room temperature.
Concrete method for breeding is identical with embodiment 1.
Instance 7:
A kind of 2 committee's exigua larvae man-made feeds: in weight portion, this feed is processed by following raw material: 215 portions of corn flour, 123 parts of analysis for soybean powder, 42 parts of dusty yeasts, 29 portions of sucrose, 4 parts of sorbic acids, 30 parts of agar, 42 parts of caseins, 12 parts of B B-complexes; 3.5 part vitamin C; 0.2 portion streptomycin sulphate, 4 parts of formaldehyde, 5 parts of alcohol, 2 portions of soybean oils, 1500 parts of sterile purified waters.
Wherein B B-complex is formulated by following vitamin: 0.65 part of thiamine hydrochloride, 6.5 parts of inositols, 0.45 part in folic acid, 13 parts of Choline Chlorides, 2.5 parts of tocopherols.
The concrete configuration step of man-made feeds is:
1) component A: corn flour, analysis for soybean powder and dusty yeast are ground even, mix, make component A;
2) B component: 600 parts of distilled water → be heated to boiling → adding sorbic acid stirs → pours into, and thin rod stirs among the component A, makes B component;
3) component C:700 part distilled water → adding agar and be heated to and dissolve → add casein (will be stirred to and dissolve) → pour in the B component and stir makes component C;
4) get B B-complex, vitamin C, streptomycin sulphate and 200 parts of distilled water, in triangular flask, shake up, when treating that component C is cooled to 40 ℃, pour formaldehyde, alcohol and peanut oil into, stir, cool to room temperature.
Concrete method for breeding is identical with embodiment 1.
Embodiment 8:
A kind of 2 committee's exigua larvae man-made feeds: in weight portion, this feed is processed by following raw material: 200 portions of corn flour, 118 parts of analysis for soybean powder, 110 parts of whole wheat embryos; 40 parts of dusty yeasts, 27 portions of sucrose, 3.5 parts of sorbic acids; 28 parts of agar, 40 parts of caseins, 10 parts of B B-complexes; 2 parts of vitamin Cs; 0.1 portion streptomycin sulphate, 2.5 parts of formaldehyde, 4 parts of alcohol, 1 portion of peanut oil, 1000 parts of sterile purified waters.
Wherein B B-complex is formulated by following vitamin: 0.6 part of thiamine hydrochloride, 5 parts of inositols, 0.3 part in folic acid, 10 parts of Choline Chlorides, 1 part of tocopherol.
The concrete configuration step of man-made feeds is:
1) component A: corn flour, analysis for soybean powder, whole wheat embryo and dusty yeast are ground even, mix, make component A;
2) B component: 400 parts of distilled water → be heated to boiling → adding sorbic acid stirs → pours into, and thin rod stirs among the component A, makes B component;
3) component C:500 part distilled water → adding agar and be heated to and dissolve → add casein (be stirred to and dissolve) → pour in the B component and stir makes component C;
4) get B B-complex, vitamin C, streptomycin sulphate and 100 parts of distilled water, in triangular flask, shake up, when treating that component C is cooled to 40 ℃, pour formaldehyde, alcohol and peanut oil into, stir, cool to room temperature.
Concrete method for breeding is identical with embodiment 1.
Embodiment 9:
A kind of 2 committee's exigua larvae man-made feeds: in weight portion, this feed is processed by following raw material: 210 portions of corn flour, 121 parts of analysis for soybean powder, 41 parts of dusty yeasts, 28 portions of sucrose, 3.8 parts of sorbic acids, 29 parts of agar, 41 parts of caseins, 11 parts of B B-complexes; 3 parts of vitamin Cs; 0.15 portion streptomycin sulphate, 3.5 parts of formaldehyde, 5 parts of alcohol, 2 portions of soybean oils, 2 parts in 10% potassium hydroxide, 1250 parts of sterile purified waters.
Said B B-complex is formulated by following vitamin: 0.65 part of thiamine hydrochloride, 6 parts of inositols, 0.4 part in folic acid, 12 parts of Choline Chlorides, 2 parts of tocopherols.
The concrete configuration step of man-made feeds is:
1) component A: corn flour, analysis for soybean powder and dusty yeast are ground even, mix, make component A;
2) B component: 500 parts of distilled water → be heated to boiling → adding sorbic acid stirs → pours into, and thin rod stirs among the component A, makes B component;
3) component C:600 part distilled water → adding agar and be heated to and dissolve → add casein (be stirred to and dissolve) → pour in the B component and stir makes component C;
4) get B B-complex, vitamin C, streptomycin sulphate and 150 parts of distilled water, in triangular flask, shake up, when treating that component C is cooled to 40 ℃, pour the potassium hydroxide of formaldehyde, alcohol, soybean oil and 10% into, stir, cool to room temperature.
Concrete method for breeding is identical with embodiment 1.
Embodiment 10:
A kind of 2 committee's exigua larvae man-made feeds: in weight portion, this feed is processed by following raw material: 220 portions of corn flour, 125 parts of analysis for soybean powder, 43 parts of dusty yeasts, 29 portions of sucrose, 4 parts of sorbic acids, 30 parts of agar, 42 parts of caseins, 12 parts of B B-complexes; 3.5 part vitamin C; 0.2 portion streptomycin sulphate, 5 parts of formaldehyde, 6 parts of alcohol, 3 portions of peanut oil, 2 parts of acetate, 1500 parts of sterile purified waters.
Wherein B B-complex is formulated by following vitamin: 0.7 part of thiamine hydrochloride, 7 parts of inositols, 0.5 part in folic acid, 14 parts of Choline Chlorides, 3 parts of tocopherols.
The concrete configuration step of man-made feeds is:
1) component A: corn flour, analysis for soybean powder and dusty yeast are ground even, mix, make component A;
2) B component: 600 parts of distilled water → be heated to boiling → adding sorbic acid stirs → pours into, and thin rod stirs among the component A, makes B component;
3) component C:700 part distilled water → adding agar and be heated to and dissolve → add casein (will be stirred to and dissolve) → pour in the B component and stir makes component C;
4) get B B-complex, vitamin C, streptomycin sulphate and 200 parts of distilled water, in triangular flask, shake up, when treating that component C is cooled to 40 ℃, pour formaldehyde, alcohol, peanut oil and acetate into, stir, cool to room temperature.
Concrete method for breeding is identical with embodiment 1.
Instance 11:
A kind of 2 committee's exigua larvae man-made feeds: in weight portion, this feed is processed by following raw material: 215 portions of corn flour, 123 parts of analysis for soybean powder, 115 parts of whole wheat embryos; 42 parts of dusty yeasts, 29 portions of sucrose, 4 parts of sorbic acids; 30 parts of agar, 42 parts of caseins, 12 parts of B B-complexes; 3.5 part vitamin C; 0.2 portion streptomycin sulphate, 4 parts of formaldehyde, 5 parts of alcohol, 2 portions of soybean oils, 2 parts of acetate, 1500 parts of sterile purified waters.
Wherein B B-complex is formulated by following vitamin: 0.65 part of thiamine hydrochloride, 6.5 parts of inositols, 0.45 part in folic acid, 13 parts of Choline Chlorides, 2.5 parts of tocopherols.
The concrete configuration step of man-made feeds is:
1) component A: corn flour, analysis for soybean powder, whole wheat embryo and dusty yeast are ground even, mix, make component A;
2) B component: 600 parts of distilled water → be heated to boiling → adding sorbic acid stirs → pours into, and thin rod stirs among the component A, makes B component;
3) component C:700 part distilled water → adding agar and be heated to and dissolve → add casein (will be stirred to and dissolve) → pour in the B component and stir makes component C;
4) get B B-complex, vitamin C, streptomycin sulphate and 200 parts of distilled water, in triangular flask, shake up, when treating that component C is cooled to 40 ℃, pour formaldehyde, alcohol, peanut oil and acetate into, stir, cool to room temperature.
Concrete method for breeding is identical with embodiment 1.
Instance 12:
A kind of 2 committee's exigua larvae man-made feeds: in weight portion, this feed is processed by following raw material: 205 portions of corn flour, 120 parts of analysis for soybean powder, 41 parts of dusty yeasts, 28 portions of sucrose, 3.5 parts of sorbic acids, 29 parts of agar, 41 parts of caseins, 11 parts of B B-complexes; 3 parts of vitamin Cs; 0.1 portion streptomycin sulphate, 4 parts of formaldehyde, 5 parts of alcohol, 1 portion of peanut oil, 2 parts of acetate, 3 parts in 10% potassium hydroxide, 1000 parts of sterile purified waters.
Wherein B B-complex is formulated by following vitamin: 0.65 part of thiamine hydrochloride, 5.5 parts of inositols, 0.35 part in folic acid, 11 parts of Choline Chlorides, 1.5 parts of tocopherols.
The concrete configuration step of man-made feeds is:
1) component A: corn flour, analysis for soybean powder and dusty yeast are ground even, mix, make component A;
2) B component: 400 parts of distilled water → be heated to boiling → adding sorbic acid stirs → pours into, and thin rod stirs among the component A, makes B component;
3) component C:500 part distilled water → adding agar and be heated to and dissolve → add casein (will be stirred to and dissolve) → pour in the B component and stir makes component C;
4) get B B-complex, vitamin C, streptomycin sulphate and 100 parts of distilled water, in triangular flask, shake up, when treating that component C is cooled to 40 ℃, pour the potassium hydroxide of formaldehyde, alcohol, peanut oil, acetate and 10% into, stir, cool to room temperature.
Concrete method for breeding is identical with embodiment 1.
Claims (6)
1. one kind 2 committee's exigua larvae man-made feeds is characterized in that, represent with weight portion, contain following component in the said feed:
200-220 part corn flour, 118-125 part analysis for soybean powder, 40-43 part dusty yeast, 27-29 part sucrose; 3.5-4 part sorbic acid, 28-30 part agar, 40-42 part casein, 10-12 part B B-complex; 2-3.5 part vitamin C, 0.1-0.2 part streptomycin sulphate, 2.5-5 part formaldehyde; 4-6 part alcohol, 1-3 part vegetable oil, 1000-1500 part sterile purified water.
2. 2 committee's exigua larvae man-made feeds according to claim 1 is characterized in that, contain following component in the said feed:
215 portions of corn flour of 205-, 120-123 part analysis for soybean powder, 41-42 part dusty yeast, 28-29 part sucrose; 3.5-4 part sorbic acid, 29-30 part agar, 41-42 part casein, 11-12 part B B-complex; 3-3.5 part vitamin C, 0.1-0.2 part streptomycin sulphate, 3-4 part formaldehyde; 4-5 part alcohol, 1-2 part vegetable oil, 1000-1500 part sterile purified water.
3. 2 committee's exigua larvae man-made feeds according to claim 1 and 2 is characterized in that said B B-complex is formulated by following vitamin: thiamine hydrochloride 0.6-0.7 part; Inositol 5-7 part; Folic acid 0.3-0.5 part, Choline Chloride 10-14 part, tocopherol 1-3 part.
4. 2 committee's exigua larvae man-made feeds according to claim 1 and 2 is characterized in that said edible vegetable oil is peanut oil or soybean oil.
5. 2 committee's exigua larvae man-made feeds according to claim 1 and 2, its preparation method comprise that raw material grinds even, mixes, stirs, and it is characterized in that, specifically may further comprise the steps:
1) component A: corn flour, analysis for soybean powder and dusty yeast are ground even, mix, make component A;
2) B component: 400-600 part distilled water is heated to boiling, and the adding sorbic acid stirs, and solution is poured among the component A to stir again, makes B component;
3) component C: in 500-700 part distilled water, add agar and be heated to and dissolve, add casein again and be stirred to and dissolve, mixed liquor is poured in the B component stirred, make component C;
4) get B B-complex, vitamin C, streptomycin sulphate, 100-200 part distilled water, stir, when treating that component C is cooled to 38 ~ 42 ℃, pour formaldehyde, alcohol and vegetable oil into, stir, cool to room temperature.
6. according to said 2 the committee's exigua larvae man-made feeds of claim 5; It is characterized in that; It is glass bar or dactylethrae that the raw material used in the said step 1) grinds even instrument, said step 2), 3) or 4) in stirring tool be aseptic glass bar or the aseptic thin stick washed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103139816A CN102835596B (en) | 2012-08-30 | 2012-08-30 | Artificial feed of proxenus lepigone larva |
Applications Claiming Priority (1)
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103340181A (en) * | 2013-07-22 | 2013-10-09 | 山东省农业科学院植物保护研究所 | Method for artificially rearing athetis lepigone for test |
| CN108125074A (en) * | 2018-03-14 | 2018-06-08 | 河南省农业科学院植物保护研究所 | Double committee's noctuid man-made feeds and its method for breeding |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102578432A (en) * | 2012-03-27 | 2012-07-18 | 董志平 | Artificial feed preparation and simplified feeding method for Athelis lepigone larva |
| CN102630829A (en) * | 2012-04-05 | 2012-08-15 | 河北省农林科学院植物保护研究所 | Athetis lepigone larva feed and preparation method thereof |
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| CN102578432A (en) * | 2012-03-27 | 2012-07-18 | 董志平 | Artificial feed preparation and simplified feeding method for Athelis lepigone larva |
| CN102630829A (en) * | 2012-04-05 | 2012-08-15 | 河北省农林科学院植物保护研究所 | Athetis lepigone larva feed and preparation method thereof |
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| 白雪峰，李国强，刘书义，王鹏，王延玲: "二点委夜蛾的生物学特性研究初报", 《中国植保导刊》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103340181A (en) * | 2013-07-22 | 2013-10-09 | 山东省农业科学院植物保护研究所 | Method for artificially rearing athetis lepigone for test |
| CN108125074A (en) * | 2018-03-14 | 2018-06-08 | 河南省农业科学院植物保护研究所 | Double committee's noctuid man-made feeds and its method for breeding |
| CN108125074B (en) * | 2018-03-14 | 2021-02-23 | 河南省农业科学院植物保护研究所 | Artificial feed for athetis lepigone and feeding method thereof |
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