KR20000032060A - Feed component for chrysopa pallens ramber imago and method to breed chrysopa pallens ramber by using the same - Google Patents

Abstract

PURPOSE: A feed component for Chrysopa pal lens Ramber imago and a method to breed Chrysopa pal lens Ramber by using the same are provided which make possible to produce Chrysopa pal lens Ramber in a large scale which is effective to exterminate Aphididae that is difficult to exterminate and quick to obtain resistance to agricultural medicines, protect an ecosystem, and enhance people's health by reducing agricultural medicines contamination. CONSTITUTION: A method to breed Chrysopa pal lens Ramber by using a feed component comprised of 23.0-28.0 wt.% of sugar, 2.3-2.8 wt.% of brewing yeast, 18.0-21.0 wt.% of yeast extract, 8.0-11.0 wt.% of casein hydrolysate, 33-41.0 wt.% of an egg yolk, 5.5-6.7 wt.% of a silkworm powder and 0.2-0.3 wt.% of vit.C is comprised as follows: breeding Aphis gossypii Glomer pastured an egg of Chrysopa pal lens Ramber at 60-70% of humidity and 25-30°C of temperature for 25-35 days; collecting an imago hatched from the same stage; adding by mixing the feed component and refined water to be 100milliliter to a breeding container by soaking into a sponge followed by adding the imago collected; and inducing laying eggs of Chrysopa pal lens Ramber by breeding in a breeding room under the condition of 60-70wt.% of humidity and 25-30DeC. of temperature.

Description

Adult feed composition for Chilsung grass dragonfly and method for breeding Chilsung grass dragonfly using same

The present invention relates to a feed composition for mass breeding the natural dragonfly dragonfly of the aphids and a method for breeding a giant dragonfly dragonfly using the same.

Aphids (Homoptera, Aphididae) are distributed around 300 species in Korea and 3,000 species around the world, and insects parasitic on the soft branches, leaves and flowers of almost all types of plants. In temperate regions like Korea, there are migratory and non-immigrant aphids.

Aphids have a very high breeding rate because they use almost all of their energy just to eat and multiply, except when they are looking for new food or when they alternate with the host. It is usually overwintered in eggs, and everything that awakens in eggs in spring is female and reproduces most of the year. During asexual reproduction, eggs are hatched instead of laying eggs to produce offspring in the form of nymphs (young worms).

Aphids are not only direct damage to the goji in the tissues and suck the juice of the plant to weaken the plant, but also excreted honeydew, which contains a lot of sugars, to block the pore of the leaves and to prevent the growth of soot mold. It encourages and blocks sunlight that reaches chlorophyll, thereby inhibiting photosynthesis and causing great damage to viruses and bacteria.

In order to control the aphids that are causing severe damage to plants, Korea uses mainly drugs such as insecticides.

However, the control method using a drug in the control of aphids, which quickly acquires drug resistance, if the effect is weakened, it is necessary to take measures such as developing a new drug, and furthermore, the use of drugs such as insecticides is a pest that exists in nature. Insects can be killed without distinguishing them, and residual toxicity can cause new social problems such as environmental pollution.

Therefore, in recent years, in order to control aphids, a method of using environmentally friendly pest control techniques using natural enemies of aphids, ladybugs, grass dragonfly, etc. has attracted a lot of attention. There is a problem in the supply and demand to control the aphids by recovering it from the situation, a large amount of these conditions are required.

However, in mass breeding of natural enemies of aphids, the Chrysopa pallens Ramber is a predator not only in larvae but also in adulthood. As well as the hygiene management of the breeding facilities were difficult problems.

In addition, artificial feeds conventionally used for breeding Chilsung grass dragonfly, for example, feed containing male honeybee powder (drone honeybee powder) studied in Japan's Yugawa River, chemical synthetic feed formulated with 61 individual nutrients And semi-synthetic feed containing AY-65 (Ashi Beer Food Co., Ltd., Tokyo), honey, and purified water must supply water separately, make bulk purchase of materials difficult, feed preparation complex, and expensive reagents. There is a problem in that it is not economical.

Therefore, the present inventors have conducted research to solve the above problems in mass rearing Chilsung grass dragonfly, based on the composition of the body of the aphid, a natural feed of Chilsung grass dragonfly, the main components of egg yolk, silkworm powder As a result of preparing the feed, it was found that the seven-star grass dragonfly can be effectively raised and completed the present invention.

Therefore, it is an object of the present invention to provide an adult feed composition for adults with seven-star grass dragonfly for breeding large amounts of dragon-fly dragonfly.

It is another object of the present invention to provide a method for rearing adult insects of the seven-stared grass dragonfly using the above feed composition.

1 is a process chart showing a process of artificial breeding Chilsung grass dragonfly.

In order to achieve the above object, the feed composition of the present invention is 23.0 to 28.0 wt% of sugar, 2.3 to 2.8 wt% of brewing yeast, 18.0 to 21.0 wt% of yeast extract, 8.0 to casein hydrolysate based on the total weight of the composition 11.0 wt%, egg yolk 33-41.0 wt%, silkworm powder 5.5-6.7 wt%, and vitamin C 0.2-0.3 wt%.

In addition, the breeding method of the present invention, (1) after spinning the eggs of the seven-star grass dragonfly on cotton aphids, breeding for 25 to 35 days at 60 to 70% humidity, 25 to 30 ℃ temperature conditions;

(2) recovering the adult from the first step;

(3) diluting the feed composition of the present invention with purified water to about 100 ml, and then adding it to a breeding container by soaking it in a sponge;

(4) adding the adult recovered in step (2) to the breeding container of step (3); And

(5) incubating the breeding container in a breeding room set at a temperature condition of 60 to 70% humidity and 25 to 30 ° C. to induce spawning of the seven-stared grass dragonfly;

Characterized in that made.

Hereinafter, the present invention will be described in detail.

Optimal nutrition of Chilsung grass dragonfly revealed by Niijima (1990) based on the composition of aphids on the basis of the fact that the adult artificial feed composition of Chilsung grass dragonfly of the present invention requires aphids because adults of Chilsung grass dragonfly are predatory. It is an ingredient that can be easily obtained from the composition, and it is prepared to show an increased effect on the number of eggs and hatching rate according to feeding. It is 23.0 to 28.0 wt% of sugar and 2.3 to 2.8 wt% of brewing yeast based on the total weight of the composition. %, Yeast extract 18.0-21.0 weight%, casein hydrolysate 8.0-11.0 weight%, egg yolk 33-41.0 weight%, silkworm powder 5.5-6.7 weight%, and vitamin C 0.2-0.3 weight%.

In the feed composition of the present invention, the above-mentioned ingredient is made to be about 100 ml with purified water, and stirred evenly by a mixer, and then divided into about 20 ml (400 daily feeds of Chilsung grass dragonfly) in a small bottle by -20 ° C or less. After freezing, they can be removed and used whenever necessary.

Using the feed composition of the present invention can be seen how to breed the seven-star grass dragonfly in large quantities from Figure 1, it will be described in detail step by step.

(1) spinning the eggs of the seven-stared grass dragonfly on cotton aphids and breeding for 25 to 35 days at a humidity of 60 to 70% and a temperature of 25 to 30 ° C;

(2) recovering the adult adults bypassed in the first step;

The adult can be recovered using a insect net, and the collected adult can be stored and used for up to 6 weeks in a dark room at about 14 ° C. while supplying the artificial feed of the present invention once a week.

On the other hand, in addition to the adult in the step (1), the chrysalis and dragonfly larva immediately after the formation of cocoons can also be recovered, if the pupa is recovered can be stored in the dark room of about 4 ~ 10 ℃ for up to 30 days, if necessary It can be used after inducing allegory by storing it for 15 days at 25 ℃, and recovering the seven-stared dragonfly larva immediately after cocoon formation. It can be stored for up to 6 months at 10 ℃ and can be used to induce fables if necessary. In this case, if the cocoons weighing more than 22mg, induce dormancy, and the cocoons below 19mg do not induce dormancy, but immediately store them as adults, and do not induce sleep if stored below 30 days. Store at about 4 ~ 10 ℃ in the state.

(3) diluting the feed composition of the present invention with purified water to about 100 ml, and then adding it to a breeding container by soaking it in a sponge;

The breeding container used in the present invention is not particularly limited, but, for example, a compact container having a characteristic that a groove on the periphery of the container is formed to allow air circulation, and at the same time maintain a certain humidity in the container. Can be used, the size of the breeding vessel is also not particularly limited, in the case of the compact saree 9cm in diameter and 5cm in height is suitable for breeding four pairs of Chilsung grass dragonfly. On the other hand, the feed of the present invention is supplied by soaking in a sponge, the daily feeding requirement of the four pairs of Chilsung grass dragonfly can be satisfied when using a sponge of about 5㎣.

(4) adding the adult recovered in step (2) to the breeding container of step (3); And

(5) breeding the breeding container in a breeding room set at a humidity condition of 60 to 70%, a temperature of 25 to 30 ℃ to induce the scattering of Chilsung grass dragonfly.

On the other hand, in the breeding method of the present invention, when replacing the food when the food container is placed on the glass plate on which the fluorescent lamp is turned on and the food is replaced, it is convenient to replace the food because the seven dragonfly dragonfly is attracted to light and does not run away.

Eggs of the seven-star dragonfly cultivated by the above-mentioned method are left to cure at room temperature for about 24 hours and then used for biological control immediately after storage or without storage for up to 9 days in a dark room at about 4 ° C. if necessary. It may be.

The present invention will be described in more detail with reference to the following Examples, but the present invention is not limited to these Examples.

REFERENCE EXAMPLE 1 Optimal Nutritional Composition of Chilsung Grass Dragonfly by Niijima (1990)

Furtherance content amino acid 43.5 Sugar Sucrose Sthalose (43.5) 34.88.7 cholesterol 0.5 Fatty acid myritic acid Palmitate stearic acid Oleic acid Linoleic acid Linolenic acid (3.2) 0.21.00.21.00.30.5 Organic acidic acid acid α-ketoglutaric acid succinic acid fumaric acid citric acid (1.3) 0.50.30.30.10.1

Furtherance content Vitamin Choline Chloride Inositol Thiamine (B ₁ ) Riboflavin (B ₂ ) Nicotinic Acid Pyridoxine (B ₄ ) Pantothenate Calcium Biotin Folic Acid Cobalamin (B ₁₂ ) L-Ascorbic Acid (C) p-Aminobenzene Acid Carnitine (Br) Vitamin Pβ-carotene (A) ergosterol (D) α-tocopherol (1.10) 0.30.30.020.020.050.020.050.0050.0050.00250.30.00250.00250.00250.010.0050.005 Mineral K ₂ HPO ₄ MgSO ₄ 7H ₂ ONaClCaCO ₃ FeCl ₃ 6H ₂ OZnSO ₄ 7H ₂ OCuCl ₂ 2H ₂ OMnCl ₂ 4H ₂ OKICoCl ₂ Na ₂ MoO ₄ 2H ₂ O (6.9) 4.201.910.430.200.0700.0600.0150.0100.0040.00050.0005

Reference Example 2

Based on the optimal nutritional composition of Chilsung grass dragonfly of Table 1, the present inventors prepared 9 artificial feed compositions as shown in the following Table 2, in order to artificial breeding of adults of Chilsung grass dragonfly, Survival and weight change were observed under conditions of LD (Light Dark (time given and no light)) 16: 8 and temperature 25 ± 2 ° C. The results are shown in Table 3.

ingredient Feed composition (g / 50ml in D.W.) One 2 3 4 5 6 7 8 9 honey 5.0 ­ 2.5 2.5 2.5 2.5 2.5 2.5 ­ Sugar ­ 5.0 2.5 2.5 2.5 2.5 2.5 2.5 5.0 East Hydrolysate 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Brewing Yeast 1.0 1.0 ­ 1.0 1.0 ­ ­ ­ ­ Casein hydrolysate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Egg yolk 5.0 5.0 5.0 5.0 ­ ­ 5.0 ­ ­ Egg albumin ­ ­ ­ ­ 1.0 1.0 ­ 1.0 1.0 Vitamin ^a ­ ­ ­ ­ ­ ­ 1.0 1.0 ­ Inactivated yeast ^b ­ ­ 1.0 ­ ­ 1.0 ­ ­ 1.0 ^a Vanderzant's vitamin mixture, Sigma Co. ^b inactivated by soaking in 90% alcohol for 24hrs

From Table 3, it can be seen that the second feed composition consisting of sugar, yeast hydrolysate, brewing yeast, casein hydrolysate, and egg yolk is the most efficient.

However, the above-mentioned efficiency is not a test of the adults of the seven-stared dragonfly, but the result of the test on the larvae, the inventors of the present invention, since the insects generally require a lot of protein for spawning, the content of protein As a result of measuring the spawning water and hatching rate by raising the value, the feed containing 10g of sugar per 100ml of food, 0.5g of brewing yeast, 5g of yeast hydrolysate, 1g of casein hydrolysate and 15g of egg yolk (hereinafter referred to as 'CA') Was found to be good [Table 4].

In addition, feed added silkworm powder and casein hydrolysate to the CA feed, that is, 12.2 g of sugar per 100 ml of food, 1.2 g of brewing yeast, 9.28 g of yeast hydrolysate, 4.64 g of casein hydrolysate, egg yolk A sample containing 17.4 g, silkworm powder 2.9 g and vitamin 117 mg (hereinafter referred to as 'RS') was found to further enhance the effect [Table 4].

feed Scattering electricity (day) ^a Spawning water () Incubation rate (%) Feed No. 2 in Table 2 10.0 230 / Lifetime ^D 96.0 CA 10.3 776 / lifetime 83.2 RS 13.5 1,261 / Life 96.7 a: The period from the pupae to the start of the laying of eggs. b: The adult lifespan of the seven-larvae dragonfly is about 77 days, including the laying egg.

<Example 1>

Eggplant seedlings (grown 35 days) were planted on the seedlings, and aphids were harvested by picking one eggplant leaf per week from which cotton aphis (Aphis gossypii Glomer) was generated. On the other hand, the entire surface of the net seal with a mesh of less than 100 mesh to prevent the natural enemies of aphids, such as parasitic rods or hump flies. Ten days later, when the density of aphids became high, 1,000 eggs of the seven-stared grass dragonfly were spun out per 200 strains. After spinning, the eggs were reared at a temperature of 25 to 30 ° C. and a humidity of 70%, and after 30 days, pupa and adult were collected. Adults were captured using the insect net, and the pupa was recovered from the overlapping or folded parts of the branch leaves. The results are shown in Table 5.

Breeding period Loss area Aphid period cultivation period Number of Radiation Objects % Recovery pupa imago system 30 days 12㎡ 45 days 1,000 40 20 60 2,000 25 13 38

<Example 2>

12.2 g of sugar, 1.2 g of yeast for brewing, 9.28 g of yeast extract, 4.64 g of casein hydrolysate, 17.4 g of egg yolk, 2.9 g of silkworm powder and 116 mg of vitamin C in purified water It was soaked in a sponge and put into a compact share having a diameter of 9 cm and a height of 5 cm, and then four of the seven-stared female dragonfly and male adult were recovered. Then, breeding in the breeding room maintaining the internal humidity at 65%, the temperature at 25 ℃ to induce spawning, and when spawning begins, the females are transferred to a separate breeding container for 70 days (until death) under the same conditions. It was saturated.

In the same way, instead of artificial feed, cotton aphid and male bee powder were fed to eggs of the seven-star grass dragonfly.

The egg number and hatching rate of the seven-stared grass dragonfly which was chawned by the method described above are shown in Table 6 below.

Adult food Spawning water () Incubation rate (%) Feed of the present invention 1,261 / Life 96.7 Cotton Aphid 1,136 / lifetime 82.2 Male Bee Powder 683 / lifetime 95.0

As described above, the artificial feed composition of the present invention is easy to purchase because it is prepared with a natural product easy to purchase as a main ingredient, the spawning water of Chilsung grass dragonfly and its hatching rate also aphids that are natural feed of Chilsung grass dragonfly Since it is superior, it is possible to effectively breed Chilsung grass dragonfly, and therefore, biological control technology, which is an environmentally friendly pest control technology that promotes public health by conserving ecosystems and reducing pesticide pollution of agricultural products, can be put to practical use. have.

Claims (2)

Sugar 23.0-28.0 weight%, Brewing yeast 2.3-2.8 weight%, East extract 18.0-21.0 weight%, Casein hydrolysate 8.0-11.0 weight%, Egg yolk 33-41.0 weight%, Silkworm powder 5.5- An adult feed composition for Chrysopa pallens Ramber, comprising 6.7% by weight and 0.2-0.3% by weight of vitamin C. In the method of breeding seven-star grass dragonfly, (1) spinning the eggs of the seven-stared grass dragonfly on cotton aphids and breeding for 25 to 35 days at a humidity of 60 to 70% and a temperature of 25 to 30 ° C; (2) recovering the adult to emerge from the first step; (3) diluting the feed composition of claim 1 to about 100 ml with purified water, and then adding it to a breeding container by soaking it in a sponge; (4) adding the adult recovered in step (2) to the breeding container of step (3); And (5) incubating the breeding container in a breeding room set at a temperature condition of 60 to 70% humidity and 25 to 30 ° C. to induce spawning of the seven-stared grass dragonfly; How to breed Chilseong grass dragonfly characterized in that consisting of.