CN104938429A - Method for feeding orius sauteri - Google Patents
Method for feeding orius sauteri Download PDFInfo
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- CN104938429A CN104938429A CN201510373964.5A CN201510373964A CN104938429A CN 104938429 A CN104938429 A CN 104938429A CN 201510373964 A CN201510373964 A CN 201510373964A CN 104938429 A CN104938429 A CN 104938429A
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- 238000000034 method Methods 0.000 title claims abstract description 60
- 241001635523 Orius sauteri Species 0.000 title abstract description 5
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 99
- 230000017448 oviposition Effects 0.000 claims abstract description 29
- 241001635529 Orius Species 0.000 claims description 126
- 210000004681 ovum Anatomy 0.000 claims description 99
- 102000002322 Egg Proteins Human genes 0.000 claims description 65
- 108010000912 Egg Proteins Proteins 0.000 claims description 65
- 235000013339 cereals Nutrition 0.000 claims description 37
- 238000003763 carbonization Methods 0.000 claims description 35
- 230000012447 hatching Effects 0.000 claims description 19
- 238000005286 illumination Methods 0.000 claims description 18
- 241000819999 Nymphes Species 0.000 claims description 17
- 230000008859 change Effects 0.000 claims description 16
- 239000000758 substrate Substances 0.000 claims description 15
- 125000006850 spacer group Chemical group 0.000 claims description 12
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 11
- 241000209140 Triticum Species 0.000 claims description 11
- 235000021307 Triticum Nutrition 0.000 claims description 11
- 239000011521 glass Substances 0.000 claims description 11
- 230000001954 sterilising effect Effects 0.000 claims description 11
- 238000003860 storage Methods 0.000 claims description 11
- 239000003905 agrochemical Substances 0.000 claims description 7
- 240000008042 Zea mays Species 0.000 claims description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 6
- 229940098396 barley grain Drugs 0.000 claims description 6
- 235000005822 corn Nutrition 0.000 claims description 6
- 238000007664 blowing Methods 0.000 claims description 5
- 238000012376 hot air sterilization Methods 0.000 claims description 5
- 238000002791 soaking Methods 0.000 claims description 5
- 238000012423 maintenance Methods 0.000 claims description 3
- 244000068988 Glycine max Species 0.000 claims description 2
- 235000010469 Glycine max Nutrition 0.000 claims description 2
- 238000007689 inspection Methods 0.000 claims description 2
- 230000001052 transient effect Effects 0.000 claims description 2
- -1 dult Species 0.000 claims 1
- 235000013601 eggs Nutrition 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 238000009395 breeding Methods 0.000 abstract description 3
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 239000004464 cereal grain Substances 0.000 abstract 2
- 241000753145 Sitotroga cerealella Species 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 235000021332 kidney beans Nutrition 0.000 abstract 1
- 239000011159 matrix material Substances 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 18
- 235000013305 food Nutrition 0.000 description 8
- 229920000742 Cotton Polymers 0.000 description 7
- 230000008569 process Effects 0.000 description 6
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- 241000266501 Ormosia ormondii Species 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000003203 everyday effect Effects 0.000 description 4
- 230000003031 feeding effect Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 231100000225 lethality Toxicity 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 241001124076 Aphididae Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241001414989 Thysanoptera Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 238000004920 integrated pest control Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241001635552 Anthocoridae Species 0.000 description 1
- 241000238421 Arthropoda Species 0.000 description 1
- 241000255967 Helicoverpa zea Species 0.000 description 1
- 241000255777 Lepidoptera Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 241000500437 Plutella xylostella Species 0.000 description 1
- 241001074085 Scophthalmus aquosus Species 0.000 description 1
- 235000021405 artificial diet Nutrition 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
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- 238000007598 dipping method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Storage Of Fruits Or Vegetables (AREA)
- Cereal-Derived Products (AREA)
Abstract
The invention discloses a method for breeding orius sauteri. The method for breeding orius sauteri regards sitotroga cerealella olivier eggs as main fodder, kidney bean pods as a main oviposition matrix and carbonized cereal grains as main isolation media and controls the humidity in a feeding container through combination of the cereal grains and fresh kidney beans. In practice, the method has the advantages that operation is easy and convenient, and orius sauteri is ideal in development rate, long in service life, low in death rate, high in pick-up rate of adults, large in egg laying amount of adults and the like.
Description
Technical field
The invention belongs to integrated pest management field and predatory natural enemy insect breeding technology, relate to a kind of raising method of Orius.
Background technology
Orius Oriussauteri is a kind of at northern China distribution natural enemy insect very widely, nymph and adult all can prey on the multiple small-sized agriculture and forestry injurious insects such as thrips, aphid, aleyrodid, tetranychid in a large number, also can prey on the ovum (as cotton bollworm, diamond-back moth etc.) of lepidoptera pest, there is great using value in integrated pest management.Therefore a lot of scholar has carried out a large amount of basic research, especially artificial feeding aspect on the basis of forefathers.Current China Orius artificial diet technique and experience also very not enough, it is still in the starting stage as biological and ecological methods to prevent plant disease, pests, and erosion agent, distance large-scale production and application also have sizable gap.
Orius raises vital two conditions on a large scale: be on the one hand feed, Current Domestic many uses life food, mainly the small arthropod such as aphid, thrips, tetranychid; Oviposition substrate on the other hand, the Current Domestic live plant bud stem (as bean sprouts, kidney bean seedling stem etc.) adopting children tender more.That feed or oviposition substrate all have the shortcomings such as working condition is high, cost is high, yield poorly, the production cycle is long.In addition, in high-density rearing, Orius has internecine habit, as imappropriate process, will certainly affect feeding effect.The humid control of raising in container is also most important, and drying can cause Orius ovum and low instar nymphs lethality significantly to increase; But humidity is too high, raising chamber wall can condense the globule, and Orius is easily stuck, and is even drowned, and high humility also easily causes feed and oviposition substrate to rot to go mouldy, raise mildew spot on chamber wall simultaneously.Drawbacks such as Orius developmental rate, life-span, lethality, adult pick-up rate, Adult worms producting eggs amount be not ideal enough of using conventional husbandry methods to have.These drawbacks limit the extensive raising of Orius in all its bearings, govern the industrialization of Orius.
Summary of the invention:
For the many technical problems existed in existing Orius artificial feeding, the object of invention is one of to solve the problems of the technologies described above, further in order to the extensive raising of Orius can be developed better, promote its industrialization, inventor is through long-term experiment, invent one using gelechiid ova as main feed, using kidney bean beanpod as main oviposition substrate, using the cereal kernel through carbonization treatment as main spacer medium, control to raise the method that container humidity raises Orius with the combination of " cereal kernel---fresh beanpod ".
A kind of method of raising Orius, using gelechiid ova as main feed, using kidney bean beanpod as main oviposition substrate, using the cereal kernel through carbonization treatment as main spacer medium, control to raise container humidity with the combination of " cereal kernel---fresh beanpod " and raise Orius.
Preferably, this raising method mainly comprises the following steps:
(1) hatching of Orius ovum
Be placed in by the cereal kernel crossed through carbonization treatment and close but ventilative raising tank, the kidney bean beanpod got with Orius ovum is placed on cereal kernel, hatches;
(2) raising of Orius nymph and adult
Once find that there is the hatching of Orius nymph, evenly to throw something and feed fresh gelechiid ova in raising tank, and to throw something and feed once fresh gelechiid ova every 1-2d; The kidney bean beanpod of raising as found in tank is wilted withered and yellow or is rotted to go mouldy, and namely changes fresh kidney bean beanpod, and makes the kidney bean beanpod number in raising tank remain at 8-10 root;
(3) collection of Orius ovum
Once find that the Orius of raising 8-12% in tank turns into adult, take out all kidney bean beanpods, change fresh kidney bean beanpod simultaneously every 1-3d, the kidney bean beanpod number of raising in tank remains at 8-10 root; Kidney bean beanpod with Orius ovum can be directly used in breeds the next generation, or low temperature temporarily stores.
Preferably, in described step (1), environment of hatching temperature is 25-28 DEG C, and humidity is 60-70%, and illumination condition is 16L:8D; Described step (2) feeding environment temperature is 25-28 DEG C, and humidity is 60-70%, and illumination condition is 16L:8D; Described step (3) egg-laying environment temperature is 25-28 DEG C, and humidity is 60-70%, and illumination condition is 16L:8D.
Preferably, the control methods of described environment of hatching humidity are: the cereal kernel crossed through carbonization treatment is placed in glass and raises pot bottom, fresh kidney bean beanpod number in maintenance raising tank, all the time at 8-10 root, uses the combination of this " cereal kernel---fresh beanpod " to control to raise tank humidity.
Preferably, described raising tank tool all needs before use through hot air sterilization, and sterilising conditions is: temperature 90 DEG C, time 10h.
Preferably, the condition of described cereal kernel carbonization treatment is: temperature 200 DEG C, time 5h; After cereal kernel carbonization treatment, need spread blowing 5h in room temperature, clean environment out, described cereal kernel can be wheat seed, barley grain, corn kernel or soya bean seed.
Preferably, the initial feeding volume of described gelechiid ova is 0.5-1.0g, and along with Orius increases the length of time gradually, single gelechiid ova feeding volume is also increased to 1.0-2.0g thereupon gradually.
Preferably, described kidney bean beanpod must be fresh undamaged, and the agricultural chemicals that the Ministry of Agriculture of never contacted China prohibits the use, before use, need be that after soaking 30min in the hydrogen peroxide solution of 3%, room temperature is dried at mass body volume concentrations.
Preferably, in described step (3), the kidney bean beanpod of taking-up checked whether Orius ovum under anatomical lens and counted, under Orius ovum anatomical lens, inspection method is: ovum often originates in kidney bean beanpod edge tissues by Orius, and the marginal swell of the look that only shows money or valuables one carries unintentionally outward, intermediate recess, recessed central have the circular ovum operculum slightly swelled.
Preferably, the temporary transient condition of storage of described Orius ovum low temperature is: temperature 2-5 DEG C, humidity 90%, but the storage time can not more than 100d.
Specifically, Orius raising method of the present invention mainly comprises the following steps:
(1) hatching of Orius ovum
It is that pot bottom raised by the glass of 1500mL that 20-30g is placed in volume through the cereal kernel that carbonization treatment is crossed, and gets 8-10 root and is placed on cereal kernel with the kidney bean beanpod of Orius ovum, tie up cotton at tank mouth with rubber band.Environment of hatching temperature is 25-28 DEG C, and humidity is 60-70%, and illumination condition is 16L:8D.Every day, whether observation had Orius nymph to hatch.
(2) raising of Orius nymph and adult
Once find that there is the hatching of Orius nymph, evenly to throw something and feed fresh gelechiid ova in raising tank immediately, and to throw something and feed once fresh gelechiid ova every 1-2d.The kidney bean beanpod of raising as found in tank is wilted withered and yellow or is rotted to go mouldy, and namely changes fresh kidney bean beanpod, and makes the kidney bean beanpod number in raising tank remain at 8-10 root.Feeding environment temperature is 25-28 DEG C, and humidity is 60-70%, and illumination condition is 16L:8D.
(3) collection of Orius ovum
Once find that the Orius of raising in tank about 10% turns into adult, then change the withered and yellow or kidney bean beanpod of rot to go mouldy (comprise slight wilt withered and yellow or rot to go mouldy) of all wiltings, and take out all kidney bean beanpods every 2d, change fresh kidney bean beanpod, the kidney bean beanpod number of raising in tank remains at 8-10 root simultaneously.Egg-laying environment temperature is 25-28 DEG C, and humidity is 60-70%, and illumination condition is 16L:8D.The kidney bean beanpod of taking-up is checked whether Orius ovum under anatomical lens and has counted.Kidney bean beanpod with Orius ovum can be directly used in breeds the next generation, also can temporarily store by low temperature.
(4) spacer medium
20-30g is placed in glass through the cereal kernel that carbonization treatment is crossed and raises pot bottom, as the spacer medium of Orius in raising.
(5) regulation and control of tank humidity are raised
20-30g is placed in glass through the cereal kernel that carbonization treatment is crossed and raises pot bottom, the fresh kidney bean beanpod number in maintenance raising tank, all the time at 8-10 root, uses the combination of this " cereal kernel---fresh beanpod " to control to raise tank humidity.
Wherein, the injected volume that the Orius ovum described in step (1) often raises tank is no more than 800.
Step (1), (2), (3) described raising tank are same raising tank, namely in whole feeding process, generally do not need to change and raise tank.
This method effectively can avoid the drawbacks such as the working condition of feed and oviposition substrate in conventional husbandry methods is high, cost is high, yield poorly, the production cycle is long.This method adopts gelechiid ova as main feed, and the production technology of gelechiid ova is ripe, cost is low, output is high, with short production cycle; And main oviposition substrate, kidney bean beanpod, all can buy throughout the year, saves the operation of independent production oviposition substrate in conventional husbandry methods.This method uses the cereal kernel crossed through carbonization treatment as the spacer medium of Orius in raising, people can hide oneself for Orius for producing a large amount of space on the one hand, thus reduces its internecine probability; On the other hand; the cereal kernel of carbonization treatment, substantially not containing nutriment, even if do not had an opportunity to hatch gelechiid larva by the gelechiid ova that Orius takes food, can not find sufficient food; thus avoid gelechiid raising tank in grow, interference Orius raising operation.This method uses the combination of " cereal kernel---fresh beanpod " to control to raise tank humidity, and utilizing the water absorbing capacity of cereal kernel to stop raising top tank structure to condense out the liquid globule on the one hand affects Orius activity; Utilize fresh beanpod to discharge gaseous state moisture on the other hand and stop the too low death causing Orius of raising tank humidity.Using this method to raise in the process of Orius, generally do not need to change and raise tank, can effectively simplify the operation step.The advantages such as in practice, this method has easy and simple to handle, and Orius developmental rate is desirable, the life-span is long, lethality is low, adult pick-up rate is high, the Adult worms producting eggs phase is long, Adult worms producting eggs amount is large.
Additional aspect of the present invention and advantage will part provide in the following description, and part will become obvious from the following description, or be recognized by practice of the present invention.
Accompanying drawing explanation
Fig. 1 is embodiment 1 egg laying amount;
Fig. 2 is embodiment 2 egg laying amount;
Fig. 3 is embodiment 3 egg laying amount.
Embodiment
Below in conjunction with instantiation, the present invention is described in detail.
Embodiment 1
Using gelechiid ova as main feed, using kidney bean beanpod as main oviposition substrate, using the wheat seed through carbonization treatment as main spacer medium, control to raise container humidity with the combination of " cereal kernel---fresh beanpod " and raise Orius, raising method comprises the following steps:
(1) prepare before raising
The all utensils used in raising, through hot air sterilization, comprise 1500mL glass and raise tank, 225cm
2square cotton, rubber band and related tool etc., sterilising conditions is: temperature 90 DEG C, time 10h.
Get 35g wheat seed and carry out carbonization treatment, the condition of carbonization treatment is: temperature 200 DEG C, time 5h.After carbonization treatment, in room temperature, clean environment, spread blowing 5h out.
Never contact the kidney bean beanpod of violated agricultural chemicals from the fresh not damaged of food market by-batch purchase, carry out sterilization treatment, sterilizing methods is: be that after soaking 30min in the hydrogen peroxide solution of 3%, room temperature is dried at mass body volume concentrations.
(2) hatching of Orius ovum
Wheat seed through carbonization treatment is placed in glass and raises pot bottom, get 8 kidney bean beanpods with Orius ovum (totally 338) and be placed on wheat seed, with rubber band, cotton is tied up at tank mouth.
Environment of hatching temperature is 25.5 DEG C, and humidity is 60%, and illumination condition is 16L:8D.Every day, whether observation had Orius nymph to hatch.
(3) raising of Orius nymph and adult
At the 4th day, finding to raise in tank had Orius nymph to hatch, and evenly throw something and feed fresh gelechiid ova 0.5g immediately in each raising tank.To throw something and feed once fresh gelechiid ova every 2d afterwards, along with Orius increases the length of time gradually, single gelechiid ova feeding volume is also increased to 2.0g thereupon gradually.
At the 7th day, finding to raise in tank had the wilting of part kidney bean beanpod withered and yellow, namely changes fresh kidney bean beanpod.After this once find that the kidney bean beanpod of raising in tank is wilted withered and yellow or rots to go mouldy, namely change fresh kidney bean beanpod, and make the kidney bean beanpod number in raising tank remain at 8.
Feeding environment temperature is 25.5 DEG C, and humidity is 60%, and illumination condition is 16L:8D.
(4) collection of Orius ovum
At fortnight, find that the Orius of raising in tank about 10% turns into adult, then change the withered and yellow or kidney bean beanpod of rot to go mouldy (comprise slight wilt withered and yellow or rot to go mouldy) of all wiltings, and take out all kidney bean beanpods every 2d, change fresh kidney bean beanpod simultaneously, make the kidney bean beanpod number in raising tank remain at 8.
Egg-laying environment temperature is 25.5 DEG C, and humidity is 60%, and illumination condition is 16L:8D.
The kidney bean beanpod of taking-up is checked whether Orius ovum under anatomical lens and has counted, method is: ovum often originates in kidney bean beanpod edge tissues by Orius, and the marginal swell of the look that only shows money or valuables one carries unintentionally outward, intermediate recess, recessed central have the circular ovum operculum slightly swelled.Total egg laying amount is 1401 (see Fig. 1).
(5) hatching of filial generation Orius ovum
The kidney bean beanpod with Orius ovum that step (4) obtains can be directly used in breeds the next generation, the same step of method (2).
(6) low temperature of filial generation Orius ovum temporarily stores
The kidney bean beanpod with Orius ovum that step (4) obtains can temporarily store by low temperature, and condition of storage is: temperature 2 DEG C, humidity 90%, the storage time is no more than 100d.
Embodiment 2
Using gelechiid ova as main feed, using kidney bean beanpod as main oviposition substrate, using the barley grain through carbonization treatment as main spacer medium, control to raise container humidity with the combination of " cereal kernel---fresh beanpod " and raise Orius, raising method comprises the following steps:
(1) prepare before raising
The all utensils used in raising, through hot air sterilization, comprise 1500mL glass and raise tank, 225cm
2square cotton, rubber band and related tool etc., sterilising conditions is: temperature 90 DEG C, time 10h.
Get 30g barley grain and carry out carbonization treatment, the condition of carbonization treatment is: temperature 200 DEG C, time 5h.After carbonization treatment, in room temperature, clean environment, spread blowing 5h out.
From the kidney bean beanpod of the never contacted violated agricultural chemicals of the fresh not damaged of food market by-batch purchase, carry out sterilization treatment, sterilizing methods is: be that after soaking 30min in the hydrogen peroxide solution of 3%, room temperature is dried at mass body volume concentrations.
(2) hatching of Orius ovum
Barley grain through carbonization treatment is placed in glass and raises pot bottom, get 10 kidney bean beanpods with Orius ovum (totally 526) and be placed on barley grain, with rubber band, cotton is tied up at tank mouth.
Environment of hatching temperature is 27 DEG C, and humidity is 65%, and illumination condition is 16L:8D.Every day, whether observation had Orius nymph to hatch.
(3) raising of Orius nymph and adult
At the 3rd day, finding to raise in tank had Orius nymph to hatch, and evenly throw something and feed fresh gelechiid ova 0.5g immediately in each raising tank.To throw something and feed once fresh gelechiid ova every 1d afterwards, along with Orius increases the length of time gradually, single gelechiid ova feeding volume is also increased to 2.0g thereupon gradually.
At the 7th day, finding to raise in tank had the wilting of part kidney bean beanpod withered and yellow, namely changes fresh kidney bean beanpod.After this once find that the kidney bean beanpod of raising in tank is wilted withered and yellow or rots to go mouldy, namely change fresh kidney bean beanpod, and make the kidney bean beanpod number in raising tank remain at 10.
Feeding environment temperature is 27 DEG C, and humidity is 65%, and illumination condition is 16L:8D.
(4) collection of Orius ovum
At the 12 day, find that the Orius of raising in tank about 10% turns into adult, then change the withered and yellow or kidney bean beanpod of rot to go mouldy (comprise slight wilt withered and yellow or rot to go mouldy) of all wiltings, and take out all kidney bean beanpods every 2d, change fresh kidney bean beanpod simultaneously, make the kidney bean beanpod number in raising tank remain at 10.
Egg-laying environment temperature is 27 DEG C, and humidity is 65%, and illumination condition is 16L:8D.
The kidney bean beanpod of taking-up is checked whether Orius ovum under anatomical lens and has counted, method is: ovum often originates in kidney bean beanpod edge tissues by Orius, and the marginal swell of the look that only shows money or valuables one carries unintentionally outward, intermediate recess, recessed central have the circular ovum operculum slightly swelled.Total egg laying amount is 2033 (see Fig. 2).
(5) hatching of filial generation Orius ovum
The kidney bean beanpod with Orius ovum that step (4) obtains can be directly used in breeds the next generation, the same step of method (2).
(6) low temperature of filial generation Orius ovum temporarily stores
The kidney bean beanpod with Orius ovum that step (4) obtains can temporarily store by low temperature, and condition of storage is: temperature 4 DEG C, humidity 90%, the storage time is no more than 100d.
Embodiment 3
Using gelechiid ova as main feed, using kidney bean beanpod as main oviposition substrate, using the corn kernel through carbonization treatment as main spacer medium, control to raise container humidity with the combination of " cereal kernel---fresh beanpod " and raise Orius, raising method comprises the following steps:
(1) prepare before raising
The all utensils used in raising, through hot air sterilization, comprise 1500mL glass and raise tank, 225cm
2square cotton, rubber band and related tool etc., sterilising conditions is: temperature 90 DEG C, time 10h.
Get 30g corn kernel and carry out carbonization treatment, the condition of carbonization treatment is: temperature 200 DEG C, time 5h.After carbonization treatment, in room temperature, clean environment, spread blowing 5h out.
Never contact the kidney bean beanpod of violated agricultural chemicals from the fresh not damaged of food market by-batch purchase, carry out sterilization treatment, sterilizing methods is: be that after soaking 30min in the hydrogen peroxide solution of 3%, room temperature is dried at mass body volume concentrations.
(2) hatching of Orius ovum
Corn kernel through carbonization treatment is placed in glass and raises pot bottom, get 10 kidney bean beanpods with Orius ovum (totally 684) and be placed on corn kernel, with rubber band, cotton is tied up at tank mouth.
Environment of hatching temperature is 28 DEG C, and humidity is 70%, and illumination condition is 16L:8D.Every day, whether observation had Orius nymph to hatch.
(3) raising of Orius nymph and adult
At the 3rd day, finding to raise in tank had Orius nymph to hatch, and evenly throw something and feed fresh gelechiid ova 0.5g immediately in each raising tank.To throw something and feed once fresh gelechiid ova every 1d afterwards, along with Orius increases the length of time gradually, single gelechiid ova feeding volume is also increased to 2.0g thereupon gradually.
At the 6th day, finding to raise in tank had part kidney bean beanpod to have the trend of going mouldy of rotting, and namely changes fresh kidney bean beanpod.After this once find that the kidney bean beanpod of raising in tank is wilted withered and yellow or rots to go mouldy, namely change fresh kidney bean beanpod, and make the kidney bean beanpod number in raising tank remain at 10.
Feeding environment temperature is 28 DEG C, and humidity is 70%, and illumination condition is 16L:8D.
(4) collection of Orius ovum
At the 12 day, find that the Orius of raising in tank about 10% turns into adult, then change the withered and yellow or kidney bean beanpod of rot to go mouldy (comprise slight wilt withered and yellow or rot to go mouldy) of all wiltings, and take out all kidney bean beanpods every 2d, change fresh kidney bean beanpod simultaneously, make the kidney bean beanpod number in raising tank remain at 10.
Egg-laying environment temperature is 28 DEG C, and humidity is 70%, and illumination condition is 16L:8D.
The kidney bean beanpod of taking-up is checked whether Orius ovum under anatomical lens and has counted, method is: ovum often originates in kidney bean beanpod edge tissues by Orius, and the marginal swell of the look that only shows money or valuables one carries unintentionally outward, intermediate recess, recessed central have the circular ovum operculum slightly swelled.Total egg laying amount is 2546 (see Fig. 3).
(5) hatching of filial generation Orius ovum
The kidney bean beanpod with Orius ovum that step (4) obtains can be directly used in breeds the next generation, the same step of method (2).
(6) low temperature of filial generation Orius ovum temporarily stores
The kidney bean beanpod with Orius ovum that step (4) obtains can temporarily store by low temperature, and condition of storage is: temperature 5 DEG C, humidity 90%, the storage time is no more than 100d.
Comparative example 1
Using gelechiid ova as main feed, using kidney bean beanpod as main oviposition substrate, do not use any material as spacer medium, do not use the combination of " cereal kernel---fresh beanpod " to control to raise container humidity and raise Orius.Raise according to the Orius raising method described in embodiment 1.
Embodiment 1 and comparative example 1 are compared, finds that embodiment 1 is good compared with the feeding effect of comparative example 1.
(1) tank humidity is raised
In whole feeding process, embodiment 1 is raised without globule condensation on top tank structure, and Orius freely can be creeped on inwall; And comparative example 1 raises a large amount of globule that top tank structure condensed, a large amount of Orius is clung by the globule on inwall, and activity is extremely restricted, and even part is drowned because health is wrapped up completely by the globule.
This method uses the combination of " cereal kernel---fresh beanpod " to control to raise tank humidity, and utilizing the water absorbing capacity of cereal kernel to stop raising top tank structure to condense out the globule on the one hand affects Orius activity; Utilize fresh beanpod to discharge gaseous state moisture on the other hand and stop the too low death causing Orius of raising tank humidity.
(2) mildew putrefactive phenomenon
In whole feeding process, the raising top tank structure of embodiment 1 has no mildew, and kidney bean beanpod rots; And the raising top tank structure of comparative example 1 finding altogether 2 pieces of mildews, area is about 4.5cm altogether
2, have 7 kidney bean beanpods and rotting in various degree occur.
(3) Orius is killed each other phenomenon
Orius in embodiment 1 can be hidden oneself in the space between wheat seed, only has a small amount of because of death of killing each other; And because dead Orius quantity of killing each other is significantly more than embodiment 1 in comparative example 1, and death mostly be low instar nymphs.
(4) input and output rate
Lower formula is used to calculate " narrow sense " input and output rate of embodiment 1 and comparative example 1 respectively:
The filial generation ovum number that embodiment 1 obtains is 1401, and the parental generation ovum number of input is 338, and its " narrow sense " input and output rate is about 414.50%.
The filial generation ovum number that comparative example 1 obtains is 994, and the parental generation ovum number of input is 356, and its " narrow sense " input and output rate is about 279.21%, lower than embodiment 1.
Comparative example 2
Using gelechiid ova as main feed, using kidney bean beanpod as main oviposition substrate, using the wheat seed without carbonization treatment as main spacer medium, use the combination of " cereal kernel---fresh beanpod " to control to raise container humidity and raise Orius.Raise according to the Orius raising method described in embodiment 1.Raise according to the Orius raising method described in embodiment 1.
Embodiment 1 and comparative example 2 are compared, both discoveries feeding effect difference is not obvious.But the collection phase of the Orius ovum at comparative example 2, has a large amount of gelechiid adults, greatly disturb the raising operation of Orius.Reason is that the gelechiid larva brill that the part gelechiid ova in comparative example 2 hatches is eaten into the wheat seed without carbonization treatment and finally buds into adult.And in embodiment 1, also have part gelechiid ova to hatch gelechiid larva, but because wheat seed is through carbonization treatment mistake, therefore gelechiid larva can not find sufficient food, finally because of hungry dead, or is taken food by Orius.
Comparative example 3
Using gelechiid ova as main feed, using kidney bean beanpod as main oviposition substrate, using the wheat seed through carbonization treatment as main spacer medium, use the combination of " cereal kernel---fresh beanpod " to control to raise container humidity and raise Orius.Kidney bean beanpod is left intact before use.Raise according to the Orius raising method described in embodiment 1.Raise according to the Orius raising method described in embodiment 1.
Embodiment 1 and comparative example 3 are compared, finds that embodiment 1 is good compared with the feeding effect of comparative example 3.
Lower formula is used to calculate " narrow sense " input and output rate of embodiment 1 and comparative example 1 respectively:
The filial generation ovum number that embodiment 1 obtains is 1401, and the parental generation ovum number of input is 338, and its " narrow sense " input and output rate is about 414.50%.
The filial generation ovum number that comparative example 1 obtains is 877, and the parental generation ovum number of input is 320, and its " narrow sense " input and output rate is about 274.06%, lower than embodiment 1.
This method is through hydrogen peroxide dipping process before kidney bean beanpod uses, and one side can be killed and beanpod raised disadvantageous microorganism (as entomopathogen) and other insect worm's ovums (as other kind of minute pirate bugs etc.) to Orius; On the other hand, hydrogen peroxide (hydrogen peroxide) is strong oxidizer, by agricultural chemicals (non-violated agricultural chemicals) residual on kidney bean beanpod oxidation, can make it to lose toxicity, reduces the impact on Orius as far as possible.
Although illustrate and describe examples and comparative examples of the present invention, those having ordinary skill in the art will appreciate that: can carry out multiple change, amendment, replacement and modification to these embodiments when not departing from principle of the present invention and aim, scope of the present invention is by claim and equivalents thereof.
Claims (10)
1. raise the method for Orius for one kind, it is characterized in that, using gelechiid ova as main feed, using kidney bean beanpod as main oviposition substrate, using the cereal kernel through carbonization treatment as main spacer medium, control to raise container humidity with the combination of " cereal kernel---fresh beanpod " and raise Orius.
2. method according to claim 1, is characterized in that, key step is as follows:
(1) hatching of Orius ovum
Be placed in by the cereal kernel crossed through carbonization treatment and close but ventilative raising tank, the kidney bean beanpod got with Orius ovum is placed on cereal kernel, hatches;
(2) raising of Orius nymph and adult
Once find that there is the hatching of Orius nymph, evenly to throw something and feed fresh gelechiid ova in raising tank, and to throw something and feed once fresh gelechiid ova every 1-2 d; The kidney bean beanpod of raising as found in tank is wilted withered and yellow or is rotted to go mouldy, and namely changes fresh kidney bean beanpod, and makes the kidney bean beanpod number in raising tank remain at 8-10 root;
(3) collection of Orius ovum
Once find that the Orius of raising 8-12 % in tank turns into adult, take out all kidney bean beanpods, change fresh kidney bean beanpod simultaneously every 1-3 d, the kidney bean beanpod number of raising in tank remains at 8-10 root; Kidney bean beanpod with Orius ovum can be directly used in breeds the next generation, or low-temperature storage.
3. method according to claim 2, is characterized in that, in described step (1), environment of hatching temperature is 25-28 DEG C, and humidity is 60-70 %, and illumination condition is 16L:8D; Described step (2) feeding environment temperature is 25-28 DEG C, and humidity is 60-70 %, and illumination condition is 16L:8D; Described step (3) egg-laying environment temperature is 25-28 DEG C, and humidity is 60-70 %, and illumination condition is 16L:8D.
4. method according to claim 2, it is characterized in that, the control methods of described raising tank humidity are: the cereal kernel crossed through carbonization treatment is placed in glass and raises pot bottom, fresh kidney bean beanpod number in maintenance raising tank, all the time at 8-10 root, uses the combination of this " cereal kernel---fresh beanpod " to control to raise tank humidity.
5. method according to claim 2, is characterized in that, described raising tank tool all needs before use through hot air sterilization, and sterilising conditions is: temperature 90 DEG C, time 10h.
6. method according to claim 2, is characterized in that, the condition of described cereal kernel carbonization treatment is: temperature 200 DEG C, time 5 h; After cereal kernel carbonization treatment, need spread blowing 5 h out in room temperature, clean environment, described cereal kernel can be wheat seed, barley grain, corn kernel or soya bean seed.
7. method according to claim 2, is characterized in that, the initial feeding volume of described gelechiid ova is 0.5-1.0 g, and along with Orius increases the length of time gradually, single gelechiid ova feeding volume is also increased to 1.0-2.0 g thereupon gradually.
8. method according to claim 2, it is characterized in that, described kidney bean beanpod is fresh undamaged, and the agricultural chemicals that the Ministry of Agriculture of never contacted China prohibits the use, before use, need be that after soaking 30 min in the hydrogen peroxide solution of 3%, room temperature is dried at mass body volume concentrations.
9. method according to claim 2, it is characterized in that, in described step (3), the kidney bean beanpod of taking-up checked whether Orius ovum under anatomical lens and counted, under Orius ovum anatomical lens, inspection method is: ovum often originates in kidney bean beanpod edge tissues by Orius, and the marginal swell of the look that only shows money or valuables one carries unintentionally outward, intermediate recess, recessed central have the circular ovum operculum slightly swelled.
10. method according to claim 2, is characterized in that, the temporary transient condition of storage of described Orius ovum low temperature is: temperature 2-5 DEG C, humidity 90%, but the storage time can not more than 100d.
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| CN112997972A (en) * | 2021-03-30 | 2021-06-22 | 河南省农业科学院烟草研究所 | Ornitus avidus egg preservation method suitable for large-scale breeding |
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| CN106900656A (en) * | 2017-02-06 | 2017-06-30 | 北京市植物保护站 | A kind of cigarette fleahopper industrial production method |
| CN109329221A (en) * | 2018-12-05 | 2019-02-15 | 山东省农业科学院植物保护研究所 | A kind of method for scaled breeding of Orius |
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| CN112997972A (en) * | 2021-03-30 | 2021-06-22 | 河南省农业科学院烟草研究所 | Ornitus avidus egg preservation method suitable for large-scale breeding |
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