CN106719448A - A kind of method of indoor mass rearing tea lesser leafhopper - Google Patents
A kind of method of indoor mass rearing tea lesser leafhopper Download PDFInfo
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- CN106719448A CN106719448A CN201611099434.7A CN201611099434A CN106719448A CN 106719448 A CN106719448 A CN 106719448A CN 201611099434 A CN201611099434 A CN 201611099434A CN 106719448 A CN106719448 A CN 106719448A
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- 241001414720 Cicadellidae Species 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 29
- 230000000384 rearing effect Effects 0.000 title claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 37
- 241000819999 Nymphes Species 0.000 claims abstract description 31
- 235000013601 eggs Nutrition 0.000 claims abstract description 26
- 241000238631 Hexapoda Species 0.000 claims abstract description 18
- 235000013305 food Nutrition 0.000 claims abstract description 17
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 13
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 13
- 230000012447 hatching Effects 0.000 claims abstract description 13
- 210000004681 ovum Anatomy 0.000 claims abstract description 13
- 230000012010 growth Effects 0.000 claims abstract description 9
- 230000013011 mating Effects 0.000 claims abstract description 7
- 230000032669 eclosion Effects 0.000 claims abstract description 4
- 239000007921 spray Substances 0.000 claims abstract description 3
- 235000009024 Ceanothus sanguineus Nutrition 0.000 claims description 28
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- 235000015459 Lycium barbarum Nutrition 0.000 claims description 28
- 239000003755 preservative agent Substances 0.000 claims description 12
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- 230000003020 moisturizing effect Effects 0.000 claims description 3
- 208000024891 symptom Diseases 0.000 claims description 3
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- 238000003672 processing method Methods 0.000 claims 2
- 230000036528 appetite Effects 0.000 claims 1
- 235000019789 appetite Nutrition 0.000 claims 1
- 230000007613 environmental effect Effects 0.000 claims 1
- 238000009395 breeding Methods 0.000 abstract description 8
- 230000001488 breeding effect Effects 0.000 abstract description 8
- 238000005516 engineering process Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
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- 235000000173 Camellia sinensis var sinensis Nutrition 0.000 description 1
- 240000007524 Camellia sinensis var. sinensis Species 0.000 description 1
- 206010053759 Growth retardation Diseases 0.000 description 1
- 241000378544 Melaleuca quinquenervia Species 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
The invention discloses a kind of method of indoor mass rearing tea lesser leafhopper, the colored mud that method for breeding of the invention is impregnated with completely by the use of the tender tip of tealeaves as food source, by the use of clear water is used as cutting medium；Spawning method allows the worm natural mating to lay eggs using on the spawning branch in the tea lesser leafhopper access insect cage that will just sprout wings or sprout wings soon；After 4 days, spawning branch in insect cage is taken out, allow ovum to hatch naturally；After egg hatching, nymph takes food growth naturally on tea branch, and nymph is taken food the spray that situation addition more renews during raising according to branch, until adult eclosion；The new adult for sprouting wings is captured on new spawning branch, is laid eggs.The method of the present invention is simple to operate, cheap, can guarantee that the steady growth of population in tea lesser leafhopper room, while the different worm states and the indoor worm sources in worm age of a large amount of neat tea lesser leafhoppers can be obtained effectively.
Description
Technical field
The invention belongs to a kind of method for breeding of insect, in particular to a kind of a large amount of feedings in interior of tea lesser leafhopper
The method of supporting.
Background technology
Tea lesser leafhopper, category Semiptera Cicadellidae (Cicadellidae), is the primary pest of China tea tree, the worm master
Cause harm tea tree bud-leaf, aggrieved bud-leaf leaf margin yellowing, vein reddens, and then blade tip leaf margin atrophy is shrivelled, growth retardation, bud-leaf takes off
Fall, have a strong impact on tea yield and quality.According to statistics, the loss of middle and lower reach of Yangtze River tea area of China normal year Summer-autumn tea up to 10% ~
15%, and Serious year portion tealeaves loss up to more than 50%.
Tea lesser leafhopper is polyphagy insect, has relevant report to show that tea lesser leafhopper in addition to tealeaves is endangered, can also endanger
The plants such as beans, grassy weed, forest.The annual generation of the worm mainly receives geography, weather, ambient influnence, in the Changjiang river stream
, in the Yangtze river basin, there are for 9 ~ 11 generations in domain, the generation of south China 11 ~ 13, Hainan may be up to 17 more than generation every year.Therefore, necessary formulation
Go out the control strategy of effectively preventing tea lesser leafhopper, so as to control the harm of tea lesser leafhopper.And indoor mass rearing technology
Foundation be conducive to carrying out the Study of Prevention Technology of tea lesser leafhopper.
Tea lesser leafhopper individuality is small, body 2 ~ 3mm long, and body is again very fragile, and nymph movement is rapid, adult be good at jump and
Short distance is flown, and ovum originates in tealeaves organization internal, and these life characteristics of tea lesser leafhopper bring many tired to indoor mass rearing
Difficulty, and then hinder the further investigation to aspects such as its biology, ecological and prevention and controls.
At present, the reported in literature of tea lesser leafhopper mass rearing method is there is no, and catches a large amount of from field direct labor
Tea lesser leafhopper is not easy.From the point of view of forefathers' tea lesser leafhopper method for breeding, live body tealeaves method for breeding is mainly taken（Invention is special
Sharp 201310402498.X）Sprouted method for breeding with water planting（Patent of invention 201310212692.1）.But, both approaches are all deposited
In its limitation, so as to the purpose of mass rearing can not be reached.Wherein, live body tealeaves method for breeding need to be limited using specific device
In limited space, the tea lesser leafhopper within 5 is raised.Water planting sprout method for breeding the characteristics of be to use plant growth regulating
Agent makes tea tree sprout as the long-term food source of smaller green leaf hopper, but this method can only also raise a small amount of tea lesser leafhopper,
Because when tea lesser leafhopper population quantity increases, the speed that tea tree is sprouted far away from tea lesser leafhopper the speed that causes harm, or even tea
Branch has been withered, and tender tip is not released also.Therefore inventing a kind of method of indoor mass rearing tea lesser leafhopper has important
Meaning.
The content of the invention：
The main object of the present invention is to provide a kind of side with low cost, operating mass rearing in easy tea lesser leafhopper room
Method, in order to realize this purpose, plan takes following technical scheme：
Prepare before raising：1. tea tree tender tip：Field clip tealeaves tender tip（Branch retains 8 ~ 9 blades, cane lignifying, top
There is tender shoots in portion）, take back indoor clear water and clean one time, bottom cane（Lignifying end）It is inserted in water；Tea tree tender tip is carried after 6 days
Tea lesser leafhopper ovum will hatch completely, then with clear water rinse one time, wash the tea lesser leafhopper nymph in tea tree tender tip off.This does
Method can effectively reduce the influence that the chemical residues such as agricultural chemicals grow to tea lesser leafhopper, while avoiding outdoor population band from entering the room
In interior population.2. tea tree tender tip fixes matrix：Trained using flower muddy water, flower mud is cut into the rectangle of 2cm × 6cm × 6cm, by flower
Mud is immersed in water, and after flower mud water suction saturation, taking-up is wrapped up with preservative film, and in square side blade by the standardized road of preservative film
The openning of 2 ~ 3cm, the colored mud for wrapping up is put into the crisper added with water, and the water cover in crisper crosses preservative film otch,
Colored mud top can not be covered, it is to avoid when tea lesser leafhopper nymph creeps, be drowned in crisper, while being also convenient for being added in replacing
During fresh braches, nymph is preferably moved on fresh braches, enhances the survival rate of tea lesser leafhopper.Then crisper is put
Enter in insect cage.
Adult worms producting eggs：Use the lucite pipe of diameter 5cm, 12cm high to catch tea lesser leafhopper adult, adult is transferred to
In insect cage, allow it to match spawning naturally in tea tree tender tip, branch of having laid eggs is obtained after 4 days.Because tea lesser leafhopper in 4 days
Egg laying amount highest.Enviromental conditions for spawning：Temperature is 24 ~ 26 DEG C, and relative humidity is 60 ~ 80%, and the photoperiod is L:D=14h:10h.
The hatching of ovum：By the tea tree tender tip moisturizing culture laid eggs, sprayed 3 times with clear water daily, kept at tea tree tender tip
In high humidity state, it is to avoid branch dries up.Egg hatching condition：Temperature is 24 ~ 26 DEG C, and relative humidity is 90 ~ 100%, and the photoperiod is L:
D=14h:10h。
The raising of nymph：5 days or so rear egg hatchings, nymph takes food growth naturally in tea tree tender tip, with the increasing in worm age
Greatly, nymph food ingestion increases；Nymph is taken food the tender tip that situation addition more renews during raising according to tea tree tender tip, and nymph can be certainly
Moving is moved on to and taken food on fresh braches growth until adult eclosion；Nymph rearing conditions：Temperature is 24 ~ 26 DEG C, and relative humidity is
90 ~ 100%, the photoperiod is L:D=14h:10h.
New emerging adult：Ovum branch is taken out after 12 days, adult starts to sprout wings successively, and adult is transferred into fresh tea tree tender tip
On, for adult natural mating spawning, procreation is of future generation, is thusly-formed anniversary mass rearing.
Remarkable advantage of the invention：It is of the invention main by indoor cottage method simulation smaller green leaf hopper field survivorship environment,
Suitable tea lesser leafhopper existence and prolific rearing conditions are provided stage by stage.Method for breeding of the invention is using tealeaves
The colored mud that tender tip is impregnated with completely as food source, by the use of clear water is used as cutting medium；Spawning method is used will just sprout wings or sprout wings
Soon tea lesser leafhopper is accessed on the spawning branch in insect cage, allows the worm natural mating to lay eggs；After 4 days, take out in insect cage
Spawning branch, allows ovum to hatch naturally；After egg hatching, nymph takes food growth naturally on tea branch, according to branch quilt during nymph raising
The spray that situation addition more renews is taken food, until adult eclosion；The new adult for sprouting wings is captured on new spawning branch, is produced
Ovum.The method of the present invention is simple to operate, cheap, can guarantee that the steady growth of population in tea lesser leafhopper room, while can be with
Effectively obtain the different worm states and the indoor worm sources in worm age of a large amount of neat tea lesser leafhoppers.
Specific embodiment：
The present invention further illustrates the present invention with the following example, but protection scope of the present invention is not limited to following implementation
Example.
Embodiment 1
（1）On July 10th, 2015, outdoor tea place clip tea tree tender tip（Branch retains 8 blades, cane bottom lignifying, top
There is tender shoots）, to take back indoor clear water and clean one time, bottom cane is immersed in water, stand-by.
（2）On July 15th, 2015, colored mud is taken, flower mud is cut into thickness 2cm, and length and width are all the square of 6cm, are immersed in the water, flower
After mud water suction saturation, taking-up is wrapped up with preservative film, and will wrap the openning of the standardized road 2cm of preservative film in square side blade
The colored mud wrapped up in is put into the crisper added with water, and the water cover in crisper crosses preservative film otch, it is impossible to cover colored mud top,
Each crisper is put into two colored mud, stand-by.
（3）On July 16th, 2015, will（1）The tea branch of immersion is cleaned again, the tea lesser leafhopper nymph on removal tea branch,
It is inserted into afterwards（2）Colored mud in, each flower mud two branch of cuttage, each crisper is put into 4 branches, crisper is put into
In insect cage.
（4）Tea lesser leafhopper adult is caught from indoor population with the lucite pipe of diameter 5cm（In on July 4th, 2015
The ovum branch of taking-up catches）, adult is transferred in insect cage, allow adult natural mating to lay eggs.Enviromental conditions for spawning：Temperature is 24 DEG C,
Relative humidity is 60%, and the photoperiod is L:D=14h:10h.
（5）On July 20th, 2015 takes out the spawning tender tip in insect cage, is placed on the frame of separation net and individually raises, and takes
After going out, a water is respectively poured when daily morning, noon and dusk, the ovum in spawning tender tip can be after taking out 5 days successively
Hatching, egg hatching condition：Temperature is 24 DEG C, and relative humidity is 90%, and the photoperiod is L:D=14h:10h.
（6）On July 27th, 2015, tender tip is serious due to being caused harm, it may appear that the symptom such as withered, fallen leaves, can be aggrieved
Serious crisper is inserted into fresh tender tip, is taken food for nymph, nymph rearing conditions：Temperature is 24 DEG C, and humidity is 90%, light
Cycle is L:D=14h:10h.And old branch bar is checked, and such as do not find tea lesser leafhopper, then remove old branch bar.
（7）On August 2nd, 2015, that is, 12 days after tender tip of laying eggs taking-up, both it can be seen that adult sprouts wings successively.Now, by adult
It is transferred in new spawning tender tip, is laid eggs, procreation is of future generation.It is thusly-formed anniversary mass rearing.
Embodiment 2
（1）On July 10th, 2015, outdoor tea place clip tea tree tender tip（Branch retains 9 blades, cane bottom lignifying, top
There is tender shoots）, to take back indoor clear water and clean one time, bottom cane is immersed in water, stand-by.
（2）On July 15th, 2015, colored mud is taken, flower mud is cut into thickness 2cm, and length and width are all the square of 6cm, are immersed in the water, flower
After mud water suction saturation, taking-up is wrapped up with preservative film, and will wrap the openning of the standardized road 3cm of preservative film in square side blade
The colored mud wrapped up in is put into the crisper added with water, and the water cover in crisper crosses preservative film otch, it is impossible to cover colored mud top,
Each crisper is put into two colored mud, stand-by.
（3）On July 16th, 2015, will（1）The tea branch of immersion is cleaned again, the tea lesser leafhopper nymph on removal tea branch,
It is inserted into afterwards（2）Colored mud in, each flower mud two branch of cuttage, each crisper is put into 4 branches, crisper is put into
In insect cage.
（4）Tea lesser leafhopper adult is caught from indoor population with the lucite pipe of diameter 5cm（In on July 4th, 2015
The ovum branch of taking-up catches）, adult is transferred in insect cage, allow adult natural mating to lay eggs.Enviromental conditions for spawning：Temperature is 26 DEG C,
Relative humidity is 80%, and the photoperiod is L:D=14h:10h.
（5）On July 20th, 2015 takes out the spawning tender tip in insect cage, is placed on the frame of separation net and individually raises, and takes
After going out, a water is respectively poured when daily morning, noon and dusk, the ovum in spawning tender tip can be after taking out 5 days successively
Hatching, egg hatching condition：Temperature is 26 DEG C, and relative humidity is 100%, and the photoperiod is L:D=14h:10h.
（6）On July 27th, 2015, tender tip is serious due to being caused harm, it may appear that the symptom such as withered, fallen leaves, can be aggrieved
Serious crisper is inserted into fresh tender tip, is taken food for nymph, nymph rearing conditions：Temperature is 26 DEG C, and humidity is 100%, light
Cycle is L:D=14h:10h.And old branch bar is checked, and such as do not find tea lesser leafhopper, then remove old branch bar.
（7）On August 2nd, 2015, that is, 12 days after tender tip of laying eggs taking-up, both it can be seen that adult sprouts wings successively.Now, by adult
It is transferred in new spawning tender tip, is laid eggs, procreation is of future generation.It is thusly-formed anniversary mass rearing.
The worm amount obtained using this method is closely related with the adult quantity being discharged into insect cage：The crisper of every 10 basin
Interior, when the worm amount of release is 40, the worm amount of acquisition is 200 ~ 300, reaches spawning rate highest；When toward discharging more in insect cage
During many tea lesser leafhopper adults, egg laying amount increases, but branch is withered too fast, causes the nymph death rate higher, and worm amount is also tieed up
Hold between 200 ~ 300.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent with
Modification, should all belong to covering scope of the invention.
Claims (5)
1. a kind of method of indoor mass rearing tea lesser leafhopper, it is characterised in that comprise the following steps：
（1）Tea tree tender tip and flower mud treatment：The clip tea tree tender tip from tea tree, is cleaned one time with clear water, by the immersion of bottom cane
In clear water；After placing 6 days, then rinse one time, by branch cutting in absorbed water saturation and the colored mud that is wrapped up with preservative film, so
The colored mud that branch will be inserted with afterwards is placed in moisturizing culture in the crisper for fill clear water, and crisper is placed in standby in insect cage；
（2）Adult worms producting eggs：The adult of tea lesser leafhopper is caught with the lucite pipe of diameter 5cm, and tea lesser leafhopper is shifted
It it is 24 ~ 26 DEG C to temperature, relative humidity is 60 ~ 80%, and the photoperiod is L:D=14h:In insect cage under 10h environmental conditions, it is allowed
The natural mating spawning in tea tree tender tip；The tea tree tender tip laid eggs is collected after 4 days；
（3）The hatching of ovum：By the tea tree tender tip moisturizing culture laid eggs, sprayed 3 times with clear water daily, keep tea tree tender tip to be in
High humidity state, it is to avoid branch dries up, egg hatching condition：Temperature is 24 ~ 26 DEG C, and relative humidity is 90 ~ 100%, and the photoperiod is L:D=
14h:10h；
（4）The raising of nymph：Egg hatching after 5-7 days, nymph takes food growth, basis during nymph raising naturally in tea tree tender tip
Branch is taken food the spray that situation addition more renews, until adult eclosion；
（5）New emerging adult：The new adult for sprouting wings is gone into new tea tree tender tip to be laid eggs, is continued the cycle and is raised.
2. a kind of method of indoor mass rearing tea lesser leafhopper according to claim 1, it is characterised in that step（1）
In tea tree tender tip retain 8 ~ 9 blades for branch, cane lignifying, the tea tree tender tip of tender shoots is arranged at top.
3. the method for interior mass rearing tea lesser leafhopper as claimed in claim 1, it is characterised in that the step（1）In
Flower mud processing method be：（1）Flower mud is cut into thickness 2cm, length and width are all the square of 6cm, flower mud are immersed in water, flower mud
After water suction saturation, taking-up is wrapped up with preservative film, and will wrap the openning of the standardized 2 ~ 3cm of road of preservative film in square side blade
The colored mud wrapped up in is put into the crisper added with water；（2）When crisper adds water, the water cover in crisper is added to cross Cling film cutting
Mouthful, but colored mud top can not be covered, it is to avoid when tea lesser leafhopper nymph creeps, it is drowned in crisper, while being also convenient in more
When changing addition fresh braches, nymph is preferably moved on fresh braches, enhances the survival rate of tea lesser leafhopper.
4. the method for interior mass rearing tea lesser leafhopper as claimed in claim 1, it is characterised in that the step（4）In
Nymph raise method be：After egg hatching, nymph takes food growth naturally in tea tree tender tip, and with the increase in worm age, nymph takes
Appetite increases, and causes harm more serious, and tea branch occurs withered, fallen leaves symptom, now, can be properly added fresh braches, and nymph can be certainly
Moving is moved on to and taken food on fresh braches growth；Nymph rearing conditions：Temperature is 24 ~ 26 DEG C, and relative humidity is 90 ~ 100%, light week
Phase is L:D=14h:10h.
5. the method for interior mass rearing tea lesser leafhopper as claimed in claim 1, it is characterised in that the new emerging adult
Processing method be：Ovum branch is taken out after 12 days, adult starts to sprout wings successively, and new emerging adult is transferred in tea tree tender tip, supplies
Its natural mating is laid eggs, and procreation is of future generation, is thusly-formed anniversary mass rearing.
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| CN111443086A (en) * | 2020-04-03 | 2020-07-24 | 信阳农林学院 | Method for identifying variety of allamanda cathartica in tea garden and method for obtaining egg laying amount |
| CN113367102A (en) * | 2021-06-24 | 2021-09-10 | 广东省农业科学院茶叶研究所 | Leafhopper breeding method capable of improving breeding rate |
| CN113661964A (en) * | 2021-08-04 | 2021-11-19 | 福建省农业科学院植物保护研究所 | Method for indoor breeding tea lesser leafhoppers |
| CN115500323A (en) * | 2022-09-15 | 2022-12-23 | 中国农业科学院茶叶研究所 | Method for quickly obtaining large amount of tea lesser leafhopper nymphs |
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| CN108293965A (en) * | 2018-03-29 | 2018-07-20 | 福建省农业科学院植物保护研究所 | A kind of method of field acquisition tea lesser leafhopper |
| CN109526890A (en) * | 2019-01-14 | 2019-03-29 | 福建省农业科学院植物保护研究所 | A kind of method of interior mass rearing tea geometrid |
| CN111443086A (en) * | 2020-04-03 | 2020-07-24 | 信阳农林学院 | Method for identifying variety of allamanda cathartica in tea garden and method for obtaining egg laying amount |
| CN113367102A (en) * | 2021-06-24 | 2021-09-10 | 广东省农业科学院茶叶研究所 | Leafhopper breeding method capable of improving breeding rate |
| CN113661964A (en) * | 2021-08-04 | 2021-11-19 | 福建省农业科学院植物保护研究所 | Method for indoor breeding tea lesser leafhoppers |
| CN115500323A (en) * | 2022-09-15 | 2022-12-23 | 中国农业科学院茶叶研究所 | Method for quickly obtaining large amount of tea lesser leafhopper nymphs |
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