CN104012472B

CN104012472B - The indoor feeding method of thrips

Info

Publication number: CN104012472B
Application number: CN201410089974.1A
Authority: CN
Inventors: 和淑琪; 桂富荣; 李正跃; 严乃胜; 陈斌; 李志华; 苏国连; 蒋兴川
Original assignee: Yunnan Agricultural University
Current assignee: Yunnan Agricultural University
Priority date: 2014-03-12
Filing date: 2014-03-12
Publication date: 2016-08-17
Anticipated expiration: 2034-03-12
Also published as: CN104012472A

Abstract

The invention discloses the indoor feeding method of a kind of thrips, using the lucite pipe of both ends open as raising pipe, in raising bottom pipe, put into appropriate pollen；After the hatching of ovum grain, newly hatched larvae can be transferred to another raising pipe with tiny fur brush pen and raise, relatively large plastic containers be put into one layer of absorbent paper, is placed in growth cabinet and raises.The method of the present invention is simple to operate, price is relatively low, can be on the basis of ensureing thrips population quantity (survival rate), most clearly observe the growth promoter situation in each period of thrips, provide for the research etc. of thrips biological habit and observe data accurately, reliably.

Description

The indoor feeding method of thrips

Technical field

The invention belongs to biological field, in particular to the indoor feeding method of a kind of thrips.

Background technology

Thrips belongs to Insecta, Thysanoptera, and it mainly differentiates that morphological characteristic is that suction frustrated by mouthpart, Front and rear wing is tassel wing, has Flower-visiting habit (Palmer et al, 1989).Thysanoptera is divided into pipe Tail suborder and cone tail suborder, wherein pipe tail suborder only has a section；Cone tail suborder is containing 7 sections (Mound1995).The whole world known thrip fuana more than 7400 is planted at present, and is distributed in China Thrip fuana there are about the common thrips kind of 500 kinds of (Majid et al.2011) China report Class has: Frankliniella occidentalis, flower thrips, onion thrips, onion thrips, golden thistle horse, eight joint golden thistle horses, Yellow breast thrips etc..

The pipe tail suborder of more than 60% is distributed in Australia, mainly with fungus for food (Mound& Walker1986), only only a few kind takes food crops of causing harm (Moritzetal2001). And the Thripidae boring tail suborder has a variety of host range thrips pest species widely, they energy Enough directly cause harm, also by taking food and lay eggs multiple kinds of crops, flowers and forestry products are caused Can be caused by transmitted virus indirectly cause harm (Lewis1997).

The life cycle of thrips is made up of 5 worm states, i.e. ovum, larva (containing 2 instar larvaes), Prepupa, pupa and adult (Mound1995).Ovum is generally originated in by the thrip fuana of phytophagous plants Inside fabric texture, and in soil, carry out pupate (including prepupa and pupa), therefore these three worm The thrips of state is very difficult to (Mound&Kibby1998) observed.

The biological characteristics of thrips is very special, and they belong to diploid insecticide, and the dye of male worm Colour solid is only the half of female worm chromosome.Therefore, it is male worm that usual unfertilized ovum grain is grown, The ovum grain being fertilized then is grown for female worm (Mound&Kibby1998).Typically, thrips Sex ration is 7:3(Mound1992), but some thrip fuanas the most not yet find male one-tenth Worm.Also there are some researches show that egg hatching is female worm or male worm depends on that the polypide laid eggs takes food food Quality and quantity (Crespi1988).

Owing to Frankliniella occidentalis individuality is little, and lay eggs in inside plant tissues, be correlated with doing biology In experimentation, it is very difficult to observe and record each biological development stage, especially ovum phase (such as egg laying amount, the development duration etc. of ovum).

Summary of the invention

It is an object of the invention to provide a kind of thrips indoor feeding method, in order to realize the present invention's Purpose, intends adopting the following technical scheme that

One aspect of the present invention relates to a kind of thrips indoor feeding method, it is characterised in that include as follows Step:

(1) using the lucite pipe of both ends open as raising pipe, an opening is single Layer sealing compound seals as bottom；

(2) in raising bottom pipe, appropriate pollen is put into, as the food of Frankliniella occidentalis；

(3) another opening is as top, and from opening, Frankliniella occidentalis is put into raising pipe Afterwards, seal with monolayer sealing compound immediately, then drip on this layer of sealing compound with dropper Droplet 10% sucrose solution, covers one layer of sealing compound the most again and forms two-layer sealing compound, Frankliniella occidentalis will be laid eggs in the sucrose solution between two-layer sealing compound；

(4) when the ovum grain in the sucrose solution of top reaches " blood-shot eye illness phase ", outer layer envelope is opened One jiao of chewing gum, blots sucrose solution with filter paper, it is to avoid touch ovum grain, in the position without ovum grain The lid of one 1.5ml micro centrifugal pipe of placement location, seals top, lid with sealing compound again Addition make to be formed between sealing compound activity space after a larvae hatch, it is simple to larva is from two Layer sealing compound between take out put into new raising pipe, it is also possible to avoid ovum grain in operation by Extruding, or pressurizeed by double-deck sealing compound；

(5), after the hatching of ovum grain, newly hatched larvae can be transferred to separately with tiny fur brush pen Raise pipe for one to carry out raising (can raising by single head, it is possible to bull is raised):

(6) in relatively large plastic containers (big I according to reality raise pipe number come Order), put into one layer of absorbent paper, then put one at absorbent paper one jiao and use water-moistened cotton Flower, puts in the absorbent paper of plastic containers by the pipe of raising putting into Frankliniella occidentalis, covered container Lid, is placed in growth cabinet and raises.

The method of the present invention is simple to operate, price is relatively low, it is possible to ensureing that thrips population quantity (becomes Motility rate) on the basis of, most clearly observe the growth promoter situation in each period of thrips, Research for thrips biological habit etc. provide and observe data accurately, reliably.

Detailed description of the invention

The raising material requested of Frankliniella occidentalis

The lucite pipe of both ends open (available general 50ml centrifuge tube is cut into two halves, Section sand papering is smooth), a length of 30～40mm, both ends open diameter is about 20mm；

Sealing compound (4IN. × 125FT.ROLL)；

Pollen (Flos Rosae Rugosas pollen of breaking cellular wall) and 10% sucrose solution；

The lid of 1.5ml centrifuge tube；

Plastic containers, Cotton Gossypii, absorbent paper.

Method for breeding:

(1) using the lucite pipe of both ends open as raising pipe, an opening is single Layer sealing compound seals as bottom.

(2) (pollen amount can be according to Frankliniella occidentalis worm to put into appropriate pollen in raising bottom pipe The size of mouth density is put into), as the food of Frankliniella occidentalis.

(3) another opening is as top, and from opening, Frankliniella occidentalis is put into raising pipe (single head raising can be carried out according to requirement of experiment or bull is raised afterwards；General access for larva Or adult, because prepupa and pupa are easy to death by after external interference；And the ovum phase is in two-layer Thin film is hatched), carry out sealing that (Frankliniella occidentalis is i.e. sealed in immediately with monolayer sealing compound Raise in pipe, and directly take food pollen particles and carry out growth promoter), then with dropper at this layer Drip upper 10% sucrose solution on sealing compound, cover one layer of sealing compound the most again (i.e. Two-layer sealing compound).Frankliniella occidentalis by the sucrose solution between two-layer sealing compound is laid eggs (because of This, alternatively referred to as lay eggs end in top).

(4) (see red the phase, i.e. when the ovum grain in the sucrose solution of top reaches " blood-shot eye illness phase " Ovum grain ovum operculum one end when nearly hatching has a pile to see red point), open outer layer sealing compound one jiao, Blot sucrose solution with filter paper, avoid contact to ovum grain as far as possible, place in the position without ovum grain The lid of one 1.5ml micro centrifugal pipe, seals top with sealing compound, the addition of lid again Make to be formed between sealing compound activity space after a larvae hatch, it is simple to larva is sealed from two-layer Take out between glue and put into new raising pipe, it is also possible to avoid ovum grain to be extruded in operation, Or pressurizeed by double-deck sealing compound.

(6) in relatively large plastic containers (big I according to reality raise pipe number come Order), put into one layer of absorbent paper, then put one at absorbent paper one jiao and use water-moistened cotton Flower, puts in the absorbent paper of plastic containers by the pipe of raising putting into Frankliniella occidentalis, covered container Lid, is placed in growth cabinet and carries out breeding observing.

Application this method can clearly observe the ovum grain quantity of thrips, blood-shot eye illness phase.

The application female egg-laying time of this method list and quantity:

The average life of Frankliniella occidentalis female adult worm is (22.6 ± 1.33) d, the female one-tenth sprouted wings Worm have 1-2d preoviposition period, enter egg-laying season in egg-laying season, the most female single eggs on average amount afterwards For (76.50 ± 6.69) grain, average daily egg laying amount is (3.88 ± 0.15) grain, has in the egg-laying season 2 egg production peak times (.The development duration raised in the growth cabinet of 27 DEG C is 10.15d, Wherein one age nymph survival rate minimum 55.44%, with the method raise Frankliniella occidentalis become Worm average life is 22.60d.

The above is the preferred embodiments of the present invention, it is noted that for the art For those of ordinary skill, on the premise of without departing from principle of the present invention, it is also possible to make Some improvements and modifications, these improvements and modifications also should be regarded as protection scope of the present invention.

Claims

1.The indoor feeding method of a kind of thrips, it is characterised in that comprise the steps :

(1) Using the lucite pipe of both ends open as raising pipe, opening monolayer sealing compound is sealed as bottom；

(2) Appropriate pollen is put into, as the food of Frankliniella occidentalis in raising bottom pipe；

(3) Another opening, as top, by Frankliniella occidentalis after opening puts into raising pipe, seals with monolayer sealing compound immediately, then drips one on this layer of sealing compound with dropper 10% Sucrose solution, covers one layer of sealing compound the most again and forms two-layer sealing compound, and Frankliniella occidentalis will be laid eggs in the sucrose solution between two-layer sealing compound；

(4) When the ovum grain in the sucrose solution of top reaches " blood-shot eye illness phase ", open outer layer sealing compound one jiao, blots sucrose solution with filter paper, it is to avoid touch ovum grain, places one in the position without ovum grain 1.5ml The lid of micro centrifugal pipe, again seal top with sealing compound, the addition of lid makes to be formed between sealing compound activity space after a larvae hatch, it is easy to larva take out between two-layer sealing compound and put into new raising pipe, ovum grain can also be avoided to be extruded in operation, or pressurizeed by double-deck sealing compound；

(5) After the hatching of ovum grain, with tiny fur brush pen, newly hatched larvae can be transferred to another raising pipe and raise, single head is raised, or bull is raised；

(6) In relatively large plastic containers, container size according to reality raise pipe number order, put into one layer of absorbent paper, in absorbent paper one jiao is put one and is used water-moistened Cotton Gossypii again, the pipe of raising putting into Frankliniella occidentalis is put in the absorbent paper of plastic containers, covered container lid, is placed in growth cabinet and raises.