CN108522430B

CN108522430B - Indoor breeding method for common thrips

Info

Publication number: CN108522430B
Application number: CN201810246636.2A
Authority: CN
Inventors: 范咏梅; 孙靖雨; 姜钰婷; 陈思诚; 游佳; 白致静; 庙浩; 苏美凤
Original assignee: Hainan University
Current assignee: Hainan University
Priority date: 2018-03-23
Filing date: 2018-03-23
Publication date: 2021-02-26
Anticipated expiration: 2038-03-23
Also published as: CN108522430A

Abstract

The invention relates to an indoor breeding method of common thrips, which adopts cowpeas as feed, changes the traditional use of vegetables such as kidney beans as feed, has excellent feeding effect, adopts thicker absorbent paper to replace toilet paper as feeding packing paper, and solves the problems that the common toilet paper can be wet, soft and rotten and easily cause a great amount of death of thrips nymphs when the humidity in a bottle is too heavy. According to the method, the cowpeas are used as the feed, the sources are wide, the cowpeas are easy to cut bean sections without holes at two ends, common thrips are foraged on the surfaces of the cowpeas to lay eggs, adult pests cannot climb into the holes, bean numbing after the adult pests climb into the holes is easy to cause a large number of thrips to die, and the thrips are concentrated at two ends of the cowpeas in a second-instar nymph period, so that the thrips are collected very quickly and conveniently, and experiments show that the egg yield and the eclosion rate of the thrips are high by the method.

Description

Indoor breeding method for common thrips

Technical Field

The invention relates to an indoor breeding method of common thrips, belonging to the technical field of plant protection and insect breeding technology.

Background

The common thrips megalophrips usitatus (Bagnall), belonging to thripa Thysanoptera thrips subfamily, Thripina (Stephens) Primesner Cirsii megalophrips Bagnall, is a very important pest in agriculture and forestry. In China, common thrips is the most main pest which harms cowpeas which are important economic crops in Hainan province, seriously influences the quality of the cowpeas and greatly influences the yield and income increase of farmers.

Due to the continuous deepening of entomology research and the development of new pest control technologies, one hundred million of insects with one physiological standard need to be supplied, and the insects can be developed neatly and physiologically uniformly by artificial feeding, so the research work of insect feed becomes one of the basic technologies of entomology research and new pest control technologies. The existing common thrips indoor breeding technology has the defects that a large number of thrips often die in a short period in the breeding process and cannot be bred indoors for a long time, so that the indoor experiment is difficult to carry out.

Disclosure of Invention

The invention aims to overcome the defects of the indoor common thrips breeding technology in the prior art and provide an indoor breeding method of the common thrips.

In order to better meet the needs of scientific research experiments and to feed the thrips on a large scale, the inventor provides a method for feeding the common thrips by adopting cowpeas as feed through long-term experiments, and the method does not adopt vegetables such as kidney beans and the like as feed any more, so that the defects of feeding the common thrips (for example, the kidney beans are difficult to cut sections with no holes at two ends, adults are difficult to clean after entering cavities, the egg laying amount is low, the eclosion rate is low) caused by the problems of the feed are overcome, and the feeding effect is excellent. Secondly, the toilet paper is not used as feeding packing paper, thicker absorbent paper is used instead, the common toilet paper is too soft, which is not beneficial to pupation of the thrips, and when the humidity in the bottle is too heavy, the common toilet paper can be wet, soft and rotten, which easily causes a great amount of death of the thrips in the nymph stage.

Technical scheme

An indoor breeding method of common thrips comprises the following steps:

(1) collecting eggs: subpackaging 100-one 200-head common thistle adults with the same growth trend of eclosion on the same day by using a 200mL tissue culture bottle to serve as a mother bottle, cleaning and drying the inside of the bottle, filling absorbent paper at the bottom, putting 2-3 cleaned fresh cowpea segments, flattening two ends of the cowpea segments and avoiding holes; sealing the bottle mouth with 300 plus 400-mesh gauze, feeding for 24h, then sweeping the adult cowpea back into the bottle, taking out the cowpea segment which has spawned, replacing the cowpea segment with a new cowpea segment, putting the cowpea segment taken out into a new tissue culture bottle to be used as a sub-bottle, and the bottom of the sub-bottle is also padded with absorbent paper; taking out the cowpea segments laid eggs from the mother bottle every day, putting the cowpea segments into a new tissue culture bottle to be used as a new son bottle until adults in the mother bottle die in a large amount, and finishing the collection of the eggs;

(2) hatching eggs and raising nymphs: the thrips eggs in the sublot can naturally hatch into nymphs of 1 year after 72 hours, 1-2 sections of clean fresh cowpea sections are added every day according to the number of the nymphs in the sublot until all the nymphs in the sublot eclose into adults;

(3) subculturing: and (3) repeating the step (1) and the step (2) after the nymph emerges as an adult.

In the step (1), the thickness of the absorbent paper is 0.20-0.30 mm.

In the step (1), depressions are uniformly distributed on the absorbent paper, and preferably 10 to 15 depressions/square centimeter.

In the step (1), the specification of the cowpea segments is 5 +/-0.5 cm.

In the step (1) and the step (2), the feeding conditions in the mother bottle and the child bottle are as follows: the temperature is 28 +/-2 ℃, the relative humidity is 70 +/-5%, and the photoperiod L: D is 12: 12.

In the step (2), during the breeding process of nymphs, the cowpeas in the bottles are found to be rotten and shriveled, and are cleaned in time, so that the cleanness of the interior of the bottles is kept, and no obvious water vapor exists.

The invention has the beneficial effects that: according to the cowpea forage, the cowpea is used as the forage, the source is wide, the cowpea is easy to cut into the bean section without holes, the time is saved, the ordinary thrips forages on the surface of the cowpea to lay eggs, the problem that thrips die in large quantity due to bean replacement after adults climb into the holes is avoided, and the thrips are concentrated on two ends of the cowpea section in a large quantity during a second-instar nymph period, so that the thrips are collected quickly and conveniently; the method can culture a large amount of nymphs and adults required by the test in a short period when the population base number of the common thrips is small, and is favorable for the rapid and smooth running of the test; the paper towel can absorb the water vapor emitted by cowpeas for a long time and can not be wet and soft to rot, thereby creating a living environment with proper humidity for the thrips, and the thrips can be collected conveniently after emerging.

Detailed Description

The present invention will be described in detail with reference to specific examples.

Example 1

An indoor breeding method of common thrips comprises the following steps:

(1) collecting eggs: subpackaging 100 common adult thistle beetles which grow in the same manner and are eclosion on the same day with a 200mL tissue culture bottle to serve as a mother bottle, cleaning and drying the inside of the mother bottle, filling water absorption paper (the thickness is 0.25mm, 12 pits are uniformly distributed on the water absorption paper per square centimeter) at the bottom of the mother bottle, putting 2 cleaned fresh cowpea segments of which the length is 5 +/-0.5 cm, and flattening the two ends of the cowpea segments without holes; sealing the bottle mouth with 300-mesh gauze, feeding for 24h, then sweeping the adult cowpea back into the bottle, taking out the cowpea segment which has spawned, replacing with a new cowpea segment, putting the cowpea segment taken out into a new tissue culture bottle as a sub-bottle, and filling water absorption paper at the bottom of the sub-bottle; taking out the cowpea segments laid eggs from the mother bottle every day, putting the cowpea segments into a new tissue culture bottle to be used as a new son bottle until adults in the mother bottle die in a large amount, and finishing the collection of the eggs;

(2) hatching eggs and raising nymphs: after 72 hours, the thrips eggs in the sublot can naturally hatch into nymphs of 1 year, and after the eggs in the sublot hatch into the nymphs of 1 year, 1 section of clean fresh cowpea section is added every day until all the nymphs in the sublot eclose into adults; during the breeding process of nymphs, the cowpeas in the bottles are found to be rotten and shriveled, and are cleaned in time, so that the cleanness of the interior of the bottles is kept, and no obvious water vapor exists;

In the method, the feeding conditions in the mother bottle and the son bottle are as follows: the temperature is 28 ℃, the relative humidity is 70%, and the photoperiod L: D is 12: 12.

Comparative example 1: taking common toilet paper as packing paper as an example

100 heads of adult common thistle beetles which grow in the same way and emerge on the same day are separately packaged by a 200mL tissue culture bottle to be used as a mother bottle, the inside of the mother bottle is clean and dry, clean toilet paper is padded at the bottom, 2 sections of fresh cowpeas (two ends are flat and have no holes) which are cleaned up by 5 +/-0.5 cm are placed in the mother bottle, and the mother bottle is sealed by 300 meshes of gauze. And after 24h, sweeping the adults from the cowpea sections back into the bottles by using a writing brush, taking out the cowpea sections which have spawned, replacing the cowpea sections with new cowpea sections, putting the cowpea sections taken out into a new tissue culture bottle every day to serve as sub-bottles, and filling clean toilet paper in the sub-bottles. After the eggs in the seed bottles are hatched into nymphs of 1 year old, properly adding 1 section of clean fresh cowpeas every day until all the nymphs in the bottles emerge into adults. During the period, the mother bottle and the son bottle are kept at the temperature of 28 ℃, the relative humidity of 70 percent and the photoperiod L: D of 12: 12.

Comparative example 2: taking kidney beans as feeding materials as an example

100 common adult Cirsium japonicum with the same growth trend of eclosion on the same day are subpackaged by a 200mL tissue culture bottle to be used as a mother bottle, the bottle is clean and dry, absorbent paper is padded at the bottom, 2 sections of clean fresh kidney beans (two ends are flat and have no holes) of 5 +/-0.5 cm are put in, and the bottle is sealed by 300 meshes of gauze. After 24h, the adult insects are swept back to the bottle from the kidney bean segments by using a writing brush, the kidney bean segments which have been laid eggs are taken out and replaced by new kidney bean segments, the taken-out kidney bean segments are placed in a new tissue culture bottle every day to serve as sub-bottles, and clean hard thick paper towels are padded at the bottoms of the sub-bottles. After the eggs in the seed bottle are incubated into nymphs of 1 year, adding 1 section of clean fresh kidney beans properly every day until all the nymphs in the bottle emerge into adults. During the period, the mother bottle and the son bottle are kept at the temperature of 28 ℃, the relative humidity of 70 percent and the photoperiod L: D of 12: 12.

The egg stage (time elapsed from egg development to 1 st-year nymph), nymph development history (time elapsed from 1 st-year nymph development to adult development), egg laying amount (total egg laying amount per 100 adults from the start of experiment to the end of experiment), eclosion rate and adult life-span during the raising in examples and comparative examples were observed, and the results are shown in table 1:

TABLE 1

	Examples	Comparative example 1	Comparative example 2
				In ovo (d)	3	3	4
Nymph development calendar (d)	7(±1)	7(±1)	9(±1)
				Egg laying amount (grain/hundred heads)	16832	15733	9386
Eclosion Rate (%)	93.51	81.13	87.28
				Mean life of adult (d)	15	15	13

It can be seen from the experimental results in table 1 that the egg period and nymph development period of the common thrips using cowpea as the feed (examples and comparative example 1) are shorter than those of the common thrips using kidney bean as the feed (comparative example 2), and the egg laying amount and eclosion rate are higher by the feeding method of the present invention in example 1, which indicates that in the experimental process, the toilet paper wet rot condition is more serious in comparative example 1, and the non-porous sections are cut at both ends of the kidney bean section as much as possible in comparative example 2, but the non-porous sections are very difficult to cut and are very wasteful, and one or two kidney beans can be cut into 2-3 sections.

Claims

1. An indoor breeding method of common thrips, which is characterized by comprising the following steps:

(3) subculturing: repeating the step (1) and the step (2) after the nymph emerges as an adult;

in the step (1), the thickness of the absorbent paper is 0.20-0.30mm, the absorbent paper is uniformly distributed with depressions, and 10-15 depressions are uniformly distributed on each square centimeter of the absorbent paper.

2. An indoor feeding method of Frankliniella communis as set forth in claim 1, wherein in the step (1), the specification of the cowpea segments is 5 ± 0.5 cm.

3. An indoor feeding method of common thrips as claimed in claim 1, wherein in the step (1) and the step (2), the feeding conditions in the mother bottle and the son bottle are: temperature 28 + -2 deg.C, relative humidity 70 + -5%, and photoperiod L: D = 12: 12.

4. An indoor feeding method of common thrips as claimed in any one of claims 1 to 3, wherein in the step (2), during the feeding of nymphs, rotten and shriveled cowpeas in the bottle are found, cleaned in time, and the cleanness of the interior of the bottle is kept without obvious moisture.