CN102578052A - A bayberry orchard Drosophila elanogaster artificial breeding method, and method for breeding parasitic wasp with the same - Google Patents
A bayberry orchard Drosophila elanogaster artificial breeding method, and method for breeding parasitic wasp with the same Download PDFInfo
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Abstract
The invention discloses a bayberry orchard Drosophila elanogaster artificial breeding method, and a method for breeding parasitic wasp with the same, belonging to artificial breeding technology of natural enemy insects. The method successively propagates Pachycrepoideus vindemmiae capable of controlling bayberry orchard Drosophila elanogaster through the steps of Drosophila elanogaster breeding to obtain pupae, egg laying of Pachycrepoideus vindemmiae on the pupae, development and eclosion of parasitic wasp, and adult wasp breeding. The method is simple and convenient, and is especially suitable for large-scale indoor breeding of Pachycrepoideus vindemmiae to give large amount of wasps for test or release in bayberry orchard. Non-professional breeding personnel can grasp operation skill in short time. The breeding method has low land occupation and cost.
Description
Technical field
The present invention relates to biological breeding technology, rearing method of specifically a kind of red bayberry garden fruit bat and the step of raising parasitic wasp thereof.
Background technology
Red bayberry is one of China special product fruit tree, has long cultivation history.In recent years, red bayberry receives more and more consumers' welcome, becomes a big pillar industry of China's some hill farmer increasing both production and income of south.But along with the continuous development of red bayberry industry, damage by disease and insect is heavier day by day, and wherein, the harm of fruit bat is particularly serious; Drosophila melanogaster is a topmost fruit bat kind on the Waxberry fruit, and the fruit of being injured is rough and uneven in surface, and fruit juice is excessive, is prone to cause shedding, and red bayberry output is descended, and the article qualitative change is bad, has a strong impact on marketing fresh, storage and the processing of red bayberry.
Parasitic wasp is the important natural enemy monoid of insect, in the biological control of insect, occupies consequence.At home, we have at first found the parasitic eristalis pupa tiny golden wasp in Drosophila melanogaster pupa time.This parasitic wasp can initiatively be searched the host in the orchard, and behind the discovery host, the pin of will laying eggs inserts in the puparium, and ovum is originated between puparium and the pupal cell, and the single head parasitic wasp usually gives birth to single head honeybee ovum in pupa, and the single head offspring reaches maturity in every pupa; When parasitic, the host is benumbed death, the parasitic wasp larva reaches maturity between puparium and pupal cell; The parasitic wasp larva is a food with the Drosophila melanogaster pupal cell, develop into aged larva after, in puparium, pupate; Get out the puparium of host's Drosophila melanogaster pupa after the emergence, post-coitum is searched new host again.Every pipunculid pupa tiny golden wasp can parasitic tens of hosts, thereby have greatly reduced the Drosophila melanogaster insect population number in red bayberry garden, have reduced the harm of Drosophila melanogaster to crops such as red bayberries.Fly pupa tiny golden wasp is to one of desirable natural enemy of red bayberry garden Drosophila melanogaster control.The center at applicant place and life science institute of Anhui Normal University find fly pupa tiny golden wasp in the Ninghai on Drosophila melanogaster natural enemy bases, goodfriday grass garden such as Yuyao, system investigation Ningbo and Ninghai.Yet the domestic report of still unmatchful Drosophila melanogaster parasitic wasp at present, the harm of red bayberry garden Drosophila melanogaster becomes the key issue of red bayberry industry.Therefore, solving the artificial of fly pupa tiny golden wasp and raise on a large scale, is to realize one of effective and critical path that red bayberry garden Drosophila melanogaster continues to administer.To this key issue, we have carried out invention research to the rearing method of red bayberry garden fruit bat and parasitic wasp thereof.
Summary of the invention
Technical problem to be solved by this invention is to above-mentioned prior art present situation, and the rearing method and the step of raising parasitic wasp thereof that provide simple, raise that requisite space is less, cost is lower, can on a large scale, routinely breed a kind of red bayberry garden fruit bat of red bayberry garden fruit bat parasitic wasp.
The present invention solves the problems of the technologies described above the technical scheme that is adopted:
The rearing method of a kind of red bayberry garden fruit bat wherein, may further comprise the steps:
Step 1, the fly cage is supported in design: using 100 order nylon wires to make the length of side is 30 centimetres the fly cage main body of cube recuperating; Support fly cage lower body part and offer the first cage hole and the second cage hole respectively, will use first sleeve, one end tube edge that 100 order nylon wires make and hole, the first cage hole along stitching, the first sleeve other end tying; To use second sleeve, one end tube edge that 100 order nylon wires make and hole, the second cage hole along stitching, the second sleeve other end tying; Be placed with drosophila larvae in first sleeve and raise cup, be placed with the cup of sprouting wings in second sleeve;
Step 2, the breeding of Drosophila melanogaster and raising: in the fly cage, place the Drosophila melanogaster of growing up earlier, and place the medium of 100mL in the drosophila larvae raising cup, the slitting shape of medium in media surface, uses and sprays the bottle spray with 5ml fresh milk, and adds the orange juice of 5ml; Drosophila melanogaster can be laid eggs on medium; Change drosophila larvae in per 12 hours and raise cup; The drosophila larvae that takes out is raised cup top lid with disposal plastic cup, places and raises 5 days in the white pallet to 6 days, to larvae pupation; In the cup that above drosophila larvae is raised cup, covers every day during this time, use spray bottle spray low amounts of water;
Step 3, the collection of Drosophila melanogaster pupa: drosophila larvae can climb out of feed and in the plastic cup of glass and covering thereof, pupate before pupating, the fruit bat pupa in the plastic cup of glass and covering thereof is collected;
Step 4, the emergence of Drosophila melanogaster pupa: the part fruit bat pupa that step 3 is collected is placed in the cup of sprouting wings, and the cup of will sprouting wings is placed in second sleeve, flies into the fly cage after the emergence of fruit bat pupa.
For optimizing technique scheme, the measure of taking also comprises:
First cage hole in the above-mentioned step 1 and aperture, the second cage hole are 6 centimetres, and first sleeve and the second sleeve barrel dliameter are 6 centimetres.
Medium in the above-mentioned step 2 comprises dusty yeast 1g, agar 1.5g, propionic acid 0.7mL, sucrose 11g, corn flour 10.2g, and all the other compositions are water.
When the replacing drosophila larvae was raised cup in the above-mentioned step 2, elder generation shook foster fly cage or beats drosophila larvae and raise cup, makes fruit bat upwards fly into the fly cage, the drosophila larvae raising cup of the ready prepd adding fresh culture of rapid again replacing.
The drosophila larvae that takes out in the above-mentioned step 2 is raised cup and is raised in 20 to 28 ℃ of insectaries, and intensity of illumination is 2000lux, and the ratio at daytime and night is 14:10, and relative moisture is 50-60%.
When collecting the fruit bat pupa in the above-mentioned step 3, use the spray bottle to spray low amounts of water at the fruit bat pupa place of adhering to, after one minute, use banister bruss on wall of cup, to sweep fruit bat pupa down, the fruit bat pupa under sweeping places on the toilet paper, behind the suck dry moisture, collects.
The rearing method of the red bayberry garden fruit bat that more than one are stated is raised the step of parasitic wasp, may further comprise the steps:
Step 1; The design dependent insect cage: using 100 orders white nylon wire to make length is 30 centimetres cube dependent insect cage main body, and dependent insect cage main body side has the 3rd cage hole, uses 100 order nylon wires to make tube-shape bag; Tube-shape bag one end bag edge and hole, three cage holes be along stitching, other end tying.
Step 2; The breeding of parasitic wasp: the parasitic wasp that grows up is placed by elder generation in dependent insect cage, the fruit bat pupa of collecting is divided place in the glass culture dish, and the culture dish surface coverage has 30 order nylon wires; Then culture dish is put in the dependent insect cage; The back taking-up of one week, the corpse of the fruit bat that removing culture dish internal cause is not sprouted wings by parasitism will be collected by the fruit bat pupa of parasitism;
Step 3, the raising of parasitic wasp: will be placed temperature by the fruit bat pupa of parasitism is that 25 ℃, intensity of illumination are that the ratio at 2000lux, daytime and night is that 14:10, relative moisture are under the environment of 50-60% 18 to 20 days, promptly hatches the fully-developed parasitic wasp; The parasitic wasp of new hatching partly places in the dependent insect cage, replenishes cage endoparasitism honeybee quantity, and part is as experiment or the open-air worm source that discharges.
The diameter in the 3rd cage hole is 20 centimetres in the above-mentioned step 1, and the diameter of tube-shape bag is 20 centimetres, and length is 30 centimetres.
Compared with prior art, the rearing method of red bayberry of the present invention garden fruit bat parasitic wasp may further comprise the steps:
Step 1; The design dependent insect cage: using 100 orders white nylon wire to make length is 30 centimetres cube dependent insect cage main body, and dependent insect cage main body side has the 3rd cage hole, uses 100 order nylon wires to make tube-shape bag; Tube-shape bag one end bag edge and hole, three cage holes be along stitching, other end tying.
Step 2; The breeding of parasitic wasp: the parasitic wasp that grows up is placed by elder generation in dependent insect cage, the fruit bat pupa of collecting is divided place in the glass culture dish, and the culture dish surface coverage has 30 order nylon wires; Then culture dish is put in the dependent insect cage; The back taking-up of one week, the corpse of the fruit bat that removing culture dish internal cause is not sprouted wings by parasitism will be collected by the fruit bat pupa of parasitism;
Step 3, the raising of parasitic wasp: will be placed temperature by the fruit bat pupa of parasitism is that 25 ℃, intensity of illumination are that the ratio at 2000lux, daytime and night is that 14:10, relative moisture are under the environment of 50-60% 18 to 20 days, promptly hatches the fully-developed parasitic wasp; The parasitic wasp of new hatching partly places in the dependent insect cage, replenishes cage endoparasitism honeybee quantity, and part is as experiment or the open-air worm source that discharges.This method obtains the fly pupa through raising Drosophila melanogaster, fly pupa tiny golden wasp connects steps such as honeybee, parasitic wasp growth emergence, the raising of parasitic wasp one-tenth honeybee and successfully breeds the fly pupa tiny golden wasp of controlled red bayberry garden Drosophila melanogaster on the fruit bat pupa.But method large-scale breeding fly pupa tiny golden wasp of the present invention is realized the continuous raising of this parasitic wasp, utilizes method of the present invention to endanger serious red bayberry garden to Drosophila melanogaster a large amount of parasitic wasps are provided, to alleviate the harm that fruit bat is caused crops such as red bayberries.Drosophila melanogaster of the present invention and pupa time thereof, the rearing method of parasitic eristalis pupa tiny golden wasp was simple; Be particularly suitable for indoor mass rearing fly pupa tiny golden wasp; Obtain a large amount of parasitic wasps and be used for test or the release of red bayberry garden fly pupa tiny golden wasp; Even there is not the keeper of professional background, also can learn independent operation at short notice; The raising requisite space is less, cost is lower.But use method large-scale breeding fly pupa tiny golden wasp of the present invention; Realize the continuous breeding of this parasitic wasp; Satisfy the experimental study needs on the one hand; On the other hand, the parasitic wasp that the breeding of scale obtains can be applied to red bayberry garden or the serious orchard of other fruit bat harm, realizes biological control of pests.
Embodiment
Below embodiments of the invention are described in further detail.
The rearing method of a kind of red bayberry of the present invention garden fruit bat wherein, may further comprise the steps:
Step 1, the fly cage is supported in design: using 100 order nylon wires to make the length of side is 30 centimetres the fly cage main body of cube recuperating; Support fly cage lower body part and offer the first cage hole and the second cage hole respectively, will use first sleeve, one end tube edge that 100 order nylon wires make and hole, the first cage hole along stitching, the first sleeve other end tying; To use second sleeve, one end tube edge that 100 order nylon wires make and hole, the second cage hole along stitching, the second sleeve other end tying; Be placed with drosophila larvae in first sleeve and raise cup, be placed with the cup of sprouting wings in second sleeve; Raise cup and glass be 5.5 centimetres of diameters, high 10 centimetres glass with sprouting wings.
Step 2; The breeding of Drosophila melanogaster and raising: in the fly cage, place earlier the Drosophila melanogaster of growing up, and drosophila larvae raises a medium of placing 100mL in the cup, it is that 0.5 centimetre, width are 5 centimetres that medium is cut into thickness; Length is 10 centimetres strip; In media surface, use the spray of spray bottle with 5ml fresh milk, and add the orange juice of 5ml; Drosophila melanogaster can be laid eggs on medium; Change drosophila larvae in per 12 hours and raise cup; The drosophila larvae that takes out is raised cup top lid with disposal plastic cup, places and raises 5 days in the white pallet to 6 days, to larvae pupation; In the cup that above drosophila larvae is raised cup, covers every day during this time, use spray bottle spray low amounts of water;
Step 3; The collection of Drosophila melanogaster pupa: drosophila larvae has and before pupating, climbs out of feed and seek the habit that comparatively dry environment is pupated; So drosophila larvae can climb out of feed and in the plastic cup of glass and covering thereof, pupate before pupating, the fruit bat pupa in the plastic cup of glass and covering thereof is collected;
Step 4, the emergence of Drosophila melanogaster pupa: the part fruit bat pupa that step 3 is collected is placed in the cup of sprouting wings, and the cup of will sprouting wings is placed in second sleeve, flies into the fly cage after the emergence of fruit bat pupa.
For optimizing technique scheme, the measure of taking also comprises:
Among the embodiment, first cage hole in the step 1 and aperture, the second cage hole are 6 centimetres, and first sleeve and the second sleeve barrel dliameter are 6 centimetres.
Among the embodiment, the medium in the step 2 comprises dusty yeast 1g, agar 1.5g, propionic acid 0.7mL, sucrose 11g, corn flour 10.2g, and all the other compositions are water.
Among the embodiment, when the replacing drosophila larvae was raised cup in the step 2, elder generation shook foster fly cage or beats drosophila larvae and raise cup, makes fruit bat upwards fly into the fly cage, the drosophila larvae raising cup of the ready prepd adding fresh culture of rapid again replacing.
Among the embodiment, the drosophila larvae that takes out in the step 2 is raised cup and is raised in 20 to 28 ℃ of insectaries, and intensity of illumination is 2000lux, and the ratio at daytime and night is 14:10, and relative moisture is 50-60%.
Among the embodiment, when collecting the fruit bat pupa in the step 3, use the spray bottle to spray low amounts of water at the fruit bat pupa place of adhering to; After one minute, use banister bruss on wall of cup, to sweep fruit bat pupa down, the breakage when using this method can avoid the fruit bat pupa to take off; Fruit bat pupa under sweeping places on the toilet paper, behind the suck dry moisture, collects.
The rearing method of the red bayberry garden fruit bat that more than one are stated is raised the step of parasitic wasp, may further comprise the steps:
Step 1; The design dependent insect cage: using 100 orders white nylon wire to make length is 30 centimetres cube dependent insect cage main body, and dependent insect cage main body side has the 3rd cage hole, uses 100 order nylon wires to make tube-shape bag; Tube-shape bag one end bag edge and hole, three cage holes are along stitching, and the other end is used the rubber band tying.
Step 2, the breeding of parasitic wasp: in dependent insect cage, place earlier the parasitic wasp that grows up, the fruit bat pupa of collecting is divided to place diameter be in 90 centimetres the circular glass culture dish; The culture dish surface coverage has 30 order nylon wires; Then culture dish is put in the dependent insect cage, parasitic wasp can see through nylon wire and on the fruit bat pupa, lay eggs, and the culture dish surface coverage has nylon wire can also prevent that the fruit bat that is not sprouted wings by parasitism from disorderly flying; Sneak in the living space of parasitic wasp; The back taking-up of one week, the corpse of the fruit bat that removing culture dish internal cause is not sprouted wings by parasitism will be collected by the fruit bat pupa of parasitism;
Step 3, the raising of parasitic wasp: will be placed temperature by the fruit bat pupa of parasitism is that 25 ℃, intensity of illumination are that the ratio at 2000lux, daytime and night is that 14:10, relative moisture are under the environment of 50-60% 18 to 20 days, promptly hatches the fully-developed parasitic wasp; The parasitic wasp of new hatching partly places in the dependent insect cage, replenishes cage endoparasitism honeybee quantity, and part is as experiment or the open-air worm source that discharges.
Among the embodiment, the diameter in the 3rd cage hole is 20 centimetres in the step 1, and the diameter of tube-shape bag is 20 centimetres, and length is 30 centimetres.
Through test, use raising Drosophila melanogaster of the present invention, place 1000 of fruit bats in the single dependent insect cage, can obtain 5000 of fruit bat pupas every day; Breeding fly pupa tiny golden wasp can obtain 3000 of fly pupa tiny golden wasps every day.
This method obtains the fly pupa through raising Drosophila melanogaster, fly pupa tiny golden wasp connects steps such as honeybee, parasitic wasp growth emergence, the raising of parasitic wasp one-tenth honeybee and successfully breeds the fly pupa tiny golden wasp of controlled red bayberry garden Drosophila melanogaster on the fruit bat pupa.But large-scale breeding fly pupa tiny golden wasp of the present invention is realized the continuous raising of this parasitic wasp, and can endanger serious red bayberry garden to Drosophila melanogaster provides a large amount of parasitic wasps, to alleviate the harm that fruit bat is caused crops such as red bayberries.Drosophila melanogaster of the present invention and pupa time thereof, the rearing method of parasitic eristalis pupa tiny golden wasp was simple; Be particularly suitable for indoor mass rearing fly pupa tiny golden wasp; Obtain a large amount of parasitic wasps and be used for test or the release of red bayberry garden fly pupa tiny golden wasp; Even there is not the keeper of professional background, also can learn independent operation at short notice; The raising requisite space is less, cost is lower.But use method large-scale breeding fly pupa tiny golden wasp of the present invention; Realize the continuous breeding of this parasitic wasp; Satisfy the experimental study needs on the one hand; On the other hand, the parasitic wasp that the breeding of scale obtains can be applied to red bayberry garden or the serious orchard of other fruit bat harm, realizes biological control of pests.
Most preferred embodiment of the present invention is illustrated, and various variations or the remodeling made by those of ordinary skills can not depart from the scope of the present invention.
Claims (8)
1. the rearing method of a red bayberry garden fruit bat is characterized in that: may further comprise the steps:
Step 1, the fly cage is supported in design: using 100 order nylon wires to make the length of side is 30 centimetres the fly cage main body of cube recuperating; Support fly cage lower body part and offer the first cage hole and the second cage hole respectively, will use first sleeve, one end tube edge that 100 order nylon wires make and hole, the first cage hole along stitching, the first sleeve other end tying; To use second sleeve, one end tube edge that 100 order nylon wires make and hole, the second cage hole along stitching, the second sleeve other end tying; Be placed with drosophila larvae in described first sleeve and raise cup, be placed with the cup of sprouting wings in described second sleeve;
Step 2, the breeding of Drosophila melanogaster and raising: in the fly cage, place the Drosophila melanogaster of growing up earlier, and described drosophila larvae is raised the medium of placing 100mL in the cup; The slitting shape of medium; In media surface, use the spray of spray bottle with 5ml fresh milk, and add the orange juice of 5ml; Drosophila melanogaster can be laid eggs on medium; Change drosophila larvae in per 12 hours and raise cup; The drosophila larvae that takes out is raised cup top lid with disposal plastic cup, places and raises 5 days in the white pallet to 6 days, to larvae pupation; In the cup that above drosophila larvae is raised cup, covers every day during this time, use spray bottle spray low amounts of water;
Step 3, the collection of Drosophila melanogaster pupa: drosophila larvae can climb out of feed and in the plastic cup of glass and covering thereof, pupate before pupating, the fruit bat pupa in the plastic cup of glass and covering thereof is collected;
Step 4, the emergence of Drosophila melanogaster pupa: the part fruit bat pupa that step 3 is collected is placed in described the emergences cup, and will sprout wings and glass be placed in second sleeve, flies into the fly cage after the emergence of fruit bat pupa.
2. the rearing method of a kind of red bayberry according to claim 1 garden fruit bat is characterized in that: first cage hole in the described step 1 and aperture, the second cage hole are 6 centimetres, and described first sleeve and the second sleeve barrel dliameter are 6 centimetres.
3. the rearing method of a kind of red bayberry according to claim 2 garden fruit bat, it is characterized in that: the medium in the described step 2 comprises dusty yeast 1g, agar 1.5g, propionic acid 0.7mL, sucrose 11g, corn flour 10.2g, all the other compositions are water.
4. the rearing method of a kind of red bayberry according to claim 3 garden fruit bat; It is characterized in that: when changing drosophila larvae raising cup in the described step 2; Vibrations are earlier supported the fly cage or are beaten drosophila larvae and raise cup; Make fruit bat upwards fly into the fly cage, change the drosophila larvae of ready prepd adding fresh culture more rapidly and raise cup.
5. the rearing method of a kind of red bayberry according to claim 4 garden fruit bat; It is characterized in that: the drosophila larvae that takes out in the described step 2 is raised cup and is raised in 20 to 28 ℃ of insectaries; Intensity of illumination is 2000lux, and the ratio at daytime and night is 14:10, and relative moisture is 50-60%.
6. the rearing method of a kind of red bayberry according to claim 5 garden fruit bat; It is characterized in that: when collecting the fruit bat pupa in the described step 3, use the spray bottle to spray low amounts of water at the fruit bat pupa place of adhering to, after one minute; Use banister bruss on wall of cup, to sweep fruit bat pupa down; Fruit bat pupa under sweeping places on the toilet paper, behind the suck dry moisture, collects.
7. step of raising parasitic wasp with the rearing method of the described red bayberry of claim 1 garden fruit bat, it is characterized in that: its step comprises following:
Step 1; The design dependent insect cage: using 100 orders white nylon wire to make length is 30 centimetres cube dependent insect cage main body, and described dependent insect cage main body side has the 3rd cage hole, uses 100 order nylon wires to make tube-shape bag; Described tube-shape bag one end bag edge and hole, three cage holes be along stitching, other end tying;
Step 2; The breeding of parasitic wasp: the parasitic wasp that grows up is placed by elder generation in dependent insect cage, the fruit bat pupa of collecting is divided place in the glass culture dish, and the culture dish surface coverage has 30 order nylon wires; Then culture dish is put in the dependent insect cage; The back taking-up of one week, the corpse of the fruit bat that removing culture dish internal cause is not sprouted wings by parasitism will be collected by the fruit bat pupa of parasitism;
Step 3, the raising of parasitic wasp: will be placed temperature by the fruit bat pupa of parasitism is that 25 ℃, intensity of illumination are that the ratio at 2000lux, daytime and night is that 14:10, relative moisture are under the environment of 50-60% 18 to 20 days, promptly hatches the fully-developed parasitic wasp; The parasitic wasp of new hatching partly places in the dependent insect cage, replenishes cage endoparasitism honeybee quantity, and part is as experiment or the open-air worm source that discharges.
8. the rearing method of a kind of described red bayberry according to claim 7 garden fruit bat is raised the step of parasitic wasp, and it is characterized in that: the diameter in the 3rd cage hole is 20 centimetres in the described step 1, and the diameter of described tube-shape bag is 20 centimetres, and length is 30 centimetres.
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