CN110612945B - Device and method for stabilizing quantity of trichogramma of corn borer in field - Google Patents

Device and method for stabilizing quantity of trichogramma of corn borer in field Download PDF

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CN110612945B
CN110612945B CN201910996857.6A CN201910996857A CN110612945B CN 110612945 B CN110612945 B CN 110612945B CN 201910996857 A CN201910996857 A CN 201910996857A CN 110612945 B CN110612945 B CN 110612945B
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trichogramma
eggs
rice
rice moth
corn
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CN110612945A (en
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张学尧
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Shanxi University
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Abstract

The invention belongs to the technical field of biological pest control equipment, and particularly relates to a device and a method for stabilizing the number of trichogramma of corn borer in field. The invention aims to reduce the trichogramma release cost, stably improve the trichogramma population level in a corn field, continuously reduce the field population quantity of the corn borer and finally improve the control effect of the trichogramma. The invention comprises a bee storage device, a connecting ring, a top cover and a control device. The device disclosed by the invention is simple in structure, can realize reduction of trichogramma release cost, stably improves the trichogramma population level in the corn field, continuously reduces the field population quantity of the corn borers, and finally improves the control effect of the trichogramma.

Description

Device and method for stabilizing quantity of trichogramma of corn borer in field
Technical Field
The invention belongs to the technical field of biological pest control equipment, and particularly relates to a device and a method for stabilizing the number of trichogramma of corn borer in field.
Background
Corn is one of the most important food in the world, with corn being the major food in about one third of the world's population. In food crops, both the corn planting area and the overall yield are third in the world. The corn borer is one of the most major pests harming corn growth. The corn borer larva gnaws the corn leaves, stems and ears, which can cause the serious yield reduction of the corn. The trichogramma is a kind of egg parasitic wasp, and the host mainly includes corn borer, cotton bollworm, oriental tobacco budworm, cotton small bridgefly, black cutworm and other pests. The trichogramma has an important role in biological prevention and control work of corn pests. In the corn planting process, trichogramma is put in to prevent and control the ostrinia nubilalis, so that the trichogramma nubilalis has the advantages of no toxicity to people and livestock and no pollution to the environment, and has remarkable ecological and economic benefits.
The throwing of host eggs containing trichogramma is one of the important means for biological control of corn borers. The existing host egg releasing device and method mainly comprise the following steps: the trichogramma bee-breeding device comprises trichogramma host egg cards, various trichogramma bee-placing devices, containers of various trichogramma host egg cards, trichogramma spherical honeycombs and the like. The device and method can ensure that trichogramma can emerge from host eggs in a centralized way in a short time after the host eggs containing trichogramma are released in the field. The service life of the trichogramma imagoes after emergence is short, and the trichogramma imagoes can survive for 0.5 to 2 days generally. After the trichogramma imagoes emerge, appropriate host eggs need to be found immediately, otherwise, the trichogramma imagoes fail to be released.
The corn borers can generate a plurality of generations in one year, the generation generations increase along with the annual average temperature of the region, and the southern region of China can generate seven generations at most in one year; and the egg laying prime period of the corn borers of each generation can also be as long as 10-20 days. In order to ensure the continuous control effect of the trichogramma on the corn borer, the existing trichogramma releasing device and method need to release the trichogramma intensively, repeatedly and repeatedly during the oviposition period of the corn borer, so that the corn plants can be effectively protected from the corn borer; the prior trichogramma releasing device and method have short shelf life of trichogramma eggs and need special cold chain storage and transportation equipment, which greatly increases the biological control cost of the ostrinia nubilalis.
The key problem to be solved by the invention is to provide a device and a method capable of continuously and stably producing and releasing trichogramma adults aiming at the defects of the prior art, so that the trichogramma releasing cost is reduced, the trichogramma population level in a corn field is stably improved, the number of the trichogramma population in the corn borer field is continuously reduced, and the control effect of the trichogramma is finally improved.
Disclosure of Invention
The invention aims to reduce the release cost of trichogramma, stably improve the trichogramma population level in a corn field, continuously reduce the field population quantity of corn borers and finally improve the control effect of the trichogramma, and provides a device and a method for stabilizing the field population quantity of the corn borers. In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a device for stabilizing the quantity of trichogramma of field corn borer comprises a bee storage device, a connecting ring, a top cover and a control device, wherein the bee storage device is fixedly connected with the connecting ring, the connecting ring is fixedly connected with the top cover, the control device is arranged on the top cover, the bee storage device comprises a bottom box, the bottom box is fixedly connected with the connecting ring, a first baffle is vertically arranged in the middle of the bottom box, the bottom box is divided into a growth area and a spawning area by the first baffle, waste holes are formed in the bottom plates of the growth area and the spawning area, fan-shaped baffles are symmetrically arranged on the lower end faces of the bottom plates of the growth area and the spawning area under the waste holes, the fan-shaped baffles are rotatably arranged on the waste holes through rotating shafts, the rotating shafts are in transmission connection with output shafts of motors, after the fan-shaped baffles of the growth area are opened, 5% -50% of old feed particles and part of corn borer can still be kept inside the growth, the fan-shaped baffle can drive a rotating shaft through a motor to enable the fan-shaped baffle to rotate, a plurality of spawning plates vertical to the first baffle are arranged on a side panel positioned on the first baffle in the spawning area, a plurality of release holes are uniformly formed in the side wall of the bottom box, a grain storage box is arranged on the inner side wall of the bottom box in the growth area, a plurality of second baffles parallel to each other are arranged in the grain storage box, rice moth feed is arranged in the grain storage box, two racks are symmetrically arranged on the lower portions of the left inner side wall and the right inner side wall of the grain storage box, stop blocks are arranged at the front end and the rear end of each rack, two gears are connected to the left side and the right side of a bottom plate of the grain storage box through transmission devices, the racks are matched with the gears for use, the gears are connected with the transmission devices through the motors, the transmission devices comprise the first bevel gear, the second bevel gear and a connecting, the second bevel gear is meshed with the first bevel gear, the connecting rod penetrates through the second bevel gear, and the two gears are connected to the two ends of the connecting rod, so that the rotating speeds of the two gears are the same, the two gears walk on the corresponding racks, the aim of gradually opening the grain storage box is achieved, the rice moth feed is released stage by stage, and the effects of keeping the rice moth feed in the growth area fresh and controlling the population quantity of the rice moth in the growth area to be stable for a long time are achieved. Through designing like this and having reduced trichogrammatid release cost, stably improving trichogrammatid population level in the corn field, continuously reducing corn borer field population quantity, finally improving the prevention and cure effect of trichogrammatid, moreover the utility model discloses can maintain the basic quantity of field trichogrammatid for a long time, reduce the harm level of corn borer for a long time to reduce the input number of times of trichogrammatid, reduce the prevention and cure cost, improved biological control's efficiency greatly.
The bottom of end box is equipped with the recovery bag, and the bottom is retrieved the bag and is connected through magnetism sealing strip and end box bottom to accept growth area bottom plate and spawning area bottom plate and open the back, the corn borer excrement and urine, old fodder granule, corn borer and the trichogramma corpse that discharge out.
The egg clamp is characterized in that a connecting rod is arranged in the center of the top cover, circular rings are arranged on the upper portion and the lower portion of the connecting rod, connecting ropes are arranged on the circular rings, and egg clamps are fastened on the connecting ropes on the lower portion.
The control device comprises a solar cell panel, the solar cell panel is fixed on a top cover, a storage battery, a temperature sensing probe heating rod and a central controller are fixedly arranged on the inner wall of the top cover, the solar cell panel, the storage battery, the temperature sensing probe, the heating rod and the central controller are electrically connected, and the solar cell panel and the storage battery provide required electric power for the control system.
The lower edge of the top cover is provided with a defense ring, the insect-proof ring is arranged between the defense ring and the connecting ring at the lower part of the top cover, the defense ring can prevent external raindrops from entering, protects the insect-proof ring from being wetted by rainwater, and maintains a smooth surface.
The bottom box is in threaded connection with the connecting ring, and the connecting ring is in threaded connection with the top cover to form sealed connection, so that rice moths are prevented from escaping.
The bottom box is made of transparent materials, so that later-stage observation and maintenance are facilitated, the inner wall of a growth area in the bottom box is smooth, rice moth larvae can be prevented from climbing and escaping, the inner wall of a spawning area is rough, and the rice moth larvae after incubation climb and fall back to the growth area; the top cover is made of opaque materials, so that sunlight is prevented from being exposed; the insect-proof ring is made of glass materials and prevents predatory animals such as ants, spiders and the like from entering.
A method for stabilizing the number of trichogramma of corn borer in field comprises the following steps:
step 1, investigating the insect situation of corn borers: after the average temperature is higher than 5 ℃ every spring day every year, investigating the growth and development state of the corn borers in the corn field every seven days, and when the pupation rate of the corn borers in the overwintering generation reaches 5-10% and the lowest temperature in the field reaches 5-10 ℃, preparing to install a device for stabilizing the quantity of the corn borers trichogramma in the field;
step 2, preparing a rice moth population: respectively placing rice moth eggs, second-instar rice moth larvae, fourth-instar rice moth larvae and rice moth imagoes into a growth area, respectively placing rice moth feed particles into a grain storage box and the growth area, coating an insect repellent on an insect prevention ring while the insect repellent is hot, coating a sex attractant on a spawning plate, and inducing female rice moths to copulate and spawn at the position;
step 3, trichogramma population preparation: hanging an egg card carrying a parasitic egg of a fresh corn borer trichogramma and an egg card carrying a parasitic egg of an aged corn borer trichogramma above a growing area, and hanging an egg card carrying a parasitic egg of a fresh pine moth trichogramma and an egg card carrying a parasitic egg of an aged pine moth trichogramma above the growing area;
and 4, mounting the device: suspending a device for stabilizing the quantity of the corn borers trichogramma in the field in a shading area around the corn field according to the distance of 15-20 m and the vertical height of 0.5-2.5 m from the ground, and avoiding direct solarization by sunlight;
step 5, development and oviposition of rice moths: the method comprises the following steps of (1) enabling rice moth adults to lay eggs on a spawning plate, enabling rice moth eggs, second-instar rice moth larvae and fourth-instar rice moth larvae to grow and mature in a growth area in sequence, enabling the rice moth eggs, the second-instar rice moth larvae and the fourth-instar rice moth larvae to lay eggs on the spawning plate in sequence, so that the number of rice moth populations and the number of rice moth eggs in a device for stabilizing the number of corn borer trichogramma in a field can be maintained, and sufficient host eggs are prepared for the parasitic spawning of the corn borer trichogramma;
step 6, hatching, breeding and releasing trichogramma dendrolimi: the development starting temperature of the trichogramma dendrolimi is more than 5 ℃ and is earlier than the egg development of the trichogramma dendrolimi; the method comprises the following steps of (1) enabling parasitic eggs of aged trichogramma dendrolimi on egg cards and parasitic eggs of fresh trichogramma dendrolimi to successively grow into adult trichogramma dendrolimi, biting egg shells from the parasitic eggs, enabling the trichogramma dendrolimi to fly to an egg laying plate immediately after the trichogramma dendrolimi emerges from the shells, searching fresh eggs of the trichogramma dendrolimi on the egg laying plate, puncturing the fresh eggs of the trichogramma dendrolimi by using a tail egg laying device, laying a new generation of trichogramma dendrolimi eggs in the eggs, realizing passage of the trichogramma dendrolimi trichodermalis in a device for stabilizing the number of the trichogramma dendrolimi, enabling part of the trichogramma dendrolimi trichogramma which does not find suitable fresh eggs to be separated from the device for stabilizing the number of the trichogramma dendrolimi;
step 7, hatching, breeding and releasing the corn borers trichogramma: the development starting temperature of the corn borer trichogramma is more than 12 ℃, and the development is later than that of eggs of the pine moth trichogramma; the parasitic eggs of the old corn borer trichogramma on the egg cards and the parasitic eggs of the fresh corn borer trichogramma develop into the corn borer trichogramma imagoes in sequence, the egg shells are bitten through the parasitic eggs, the corn borer trichogramma flies to the egg laying plate immediately after emerging from the shells, the fresh rice borer eggs are searched on the egg laying plate, the fresh rice borer eggs are punctured by a tail egg laying device, new generation of corn borer trichogramma eggs are laid in the fresh rice borer eggs, passage of the corn borer trichogramma in a device for stabilizing the number of the field corn borer trichogramma is realized, part of the corn borer trichogramma which does not find the appropriate fresh rice borer eggs are separated from the device for stabilizing the number of the corn borer trichogramma through release holes, and the appropriate eggs of the corn borer are searched in the field as hosts.
The ratio of the rice moth eggs, the second-instar rice moth larvae, the fourth-instar rice moth larvae and the adult rice moth in the step 2 to the growth area is 20:2:2: 1; in the step 3, the number ratio of the fresh corn borer trichogramma to the aged corn borer trichogramma is 1:2, and the number ratio of the fresh pine moth trichogramma to the aged pine moth trichogramma is 2: 1.
The preparation method of the rice moth feed particles in the step 2 comprises the following steps:
step 1.1, according to the weight ratio: 9: 1, mixing and powdering coarse grains and fine grains according to the proportion to prepare rice moth feed powder; wherein the coarse grain is one or a mixture of more than two of rice bran, soybean meal, aged wheat, aged rice and aged corn in any proportion; the fine grain is one or a mixture of more than two of brown sugar powder, yellow mealworm powder and fish meal in any proportion;
step 1.2, after the rice moth feed powder is sieved by a 100-mesh sieve, dry heat sterilization is carried out for 30 minutes at 150 ℃, worm eggs, mould and germs in the rice moth feed powder are sterilized and inactivated, the rice moth feed powder is cooled to room temperature, and extrusion granulation is carried out to form rice moth feed particles with the diameter of 5-10 mm;
the preparation method of the insect repellent in the step 2 comprises the following steps:
selecting 3-7 parts of quercetin, 1.5-3.5 parts of xanthotoxin, 3-5 parts of 2-tridecanone and 3-9 parts of eugenol, adding 200-300 parts of 95 ℃ hot ethanol, and uniformly stirring to finish the preparation of the insect repellent;
the preparation method of the neutral attractant in the step 2 comprises the following steps:
step 2.1, mixing 15 parts of male rice moth with 40 parts of water and 55 parts of ethanol, manually homogenizing for 10 minutes by using a glass homogenizer, centrifuging for 10 minutes at 15000 r/g, taking supernatant, centrifuging for 10 minutes at 15000 r/g, and taking supernatant to obtain a crude rice moth extract;
and 2.2, mixing and stirring 100 parts of the crude extract of the rice moth with 1 part of tetradecenol acetate, 1 part of tetradecenol, 1 part of hexadecenol acetate, 1 part of hexadecenol alcohol and 100 parts of acetone uniformly to finish the preparation of the sex attractant.
Compared with the prior art, the invention has the following beneficial effects:
1. the device disclosed by the invention is simple in structure, can realize reduction of trichogramma release cost, stably improves the trichogramma population level in the corn field, continuously reduces the field population quantity of the corn borers, and finally improves the control effect of the trichogramma.
2. The method maintains the population quantity and the egg quantity of the rice moths in the device for stabilizing the quantity of the corn borers trichogramma in the field, so that the passage of the corn borers trichogramma and the pine moth trichogramma in the device is realized; the characteristic that the development starting temperature of the trichogramma dendrolimi is lower than the development starting temperature of the trichogramma corn borer is utilized to realize the sequential maturation and oviposition of the trichogramma dendrolimi and the trichogramma corn borer on the oviposition plate, and partial trichogramma corn borer and the trichogramma dendrolimi are released into a corn field sequentially through the release holes, so that the effect of stabilizing the number of the trichogramma corn borer in the field is realized.
3. According to the method, cheap feeds such as rice bran, old grains, soybean meal, brown sugar powder, yellow mealworm powder and fish meal are used as main foods, multi-generation rice moth adults can be continuously hatched in the device, the multi-generation rice moth adults can complete mating and production of rice moth eggs in the device, intermediate hosts, namely the rice moth eggs, are continuously provided for the trichogramma, and therefore the trichogramma adults are continuously produced and released.
4. The method of the invention ensures that the rice moth eggs are continuously provided by selecting the rice moth eggs, the larvae and the imagoes at different development stages as the food of the trichogramma, and lays a foundation for stabilizing the population quantity of the trichogramma, continuously releasing the trichogramma and maintaining the stable parasitism and spawning and breeding of the trichogramma.
5. According to the method, the fresh and mature trichogramma egg cards are selected simultaneously, so that the trichogramma is ensured to be continuously hatched and the emerged trichogramma is ensured to lay eggs, the supply of the trichogramma is ensured, and the fluctuation of the number of the trichogramma is reduced;
6. the method of the invention selects corn borer trichogramma and pine moth trichogramma with different growth cycle length simultaneously to hatch out the trichogramma alternately, thereby ensuring the supply of the trichogramma and reducing the fluctuation of the number of the trichogramma;
7. according to the method, two types of trichogramma which are obvious in characteristic difference such as host identification and positioning, host suitable range and the like are selected, so that the advantages of wide host range of the trichogramma dendrolimi are.
Drawings
FIG. 1 is a schematic diagram of the structure of the apparatus of the present invention;
FIG. 2 is a schematic view of the structure of the lower part of the bottom plate of the bottom case of the present invention;
FIG. 3 is a schematic view of the construction of the bin of the present invention;
FIG. 4 is a schematic view of the sliding configuration of the bin base of the present invention;
FIG. 5 is a schematic structural view of the transmission of the present invention;
FIG. 6 is a schematic structural view of a control device of the present invention;
FIG. 7 is a schematic top view of the back box of the present invention;
FIG. 8 is a schematic three-dimensional structure of the back box of the present invention;
in the figure: 1-bee storage device, 101-bottom box, 102-first clapboard, 105-spawning plate, 106 release hole, 107-grain storage box, 108-recovery bag, 109-waste hole, 110-sector baffle, 111-rotating shaft, 112-second clapboard, 113-rack, 114-gear, 115-block, 2-connecting ring, 3-top cover, 301-connecting rod, 302-circular ring, 303-connecting rope, 304-egg card, 4-control device, 401-solar panel, 402-storage battery, 403-temperature sensing probe, 404-heating rod, 405-central controller, 5-defense ring and 6-insect prevention ring.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Examples
As shown in fig. 1-8, a device for stabilizing the quantity of trichogrammatid wasps in corn borer in field comprises a wasp storage device 1, a connecting ring 2, a top cover 3 and a control device 4, wherein the wasp storage device 1 is fixedly connected with the connecting ring 2, the connecting ring 2 is fixedly connected with the top cover 3, the control device 4 is arranged on the top cover, the wasp storage device 1 comprises a bottom box 101, the bottom box 101 is fixedly connected with the connecting ring 2, a first partition plate 102 is vertically arranged at the middle part in the bottom box 101, the first partition plate 102 divides the bottom box 101 into two chambers, namely a growth area and a spawning area, waste holes 109 are arranged on the bottom plates of the growth area and the spawning area, fan-shaped baffle plates 110 are symmetrically arranged on the lower end surfaces of the bottom plates of the growth area and the spawning area under the waste holes 109, the fan-shaped baffle plates 110 are rotatably arranged on the waste holes 109 through a rotating shaft 111, the rotating shaft, the side panel that lies in No. one baffle 102 in the spawning zone is equipped with a plurality of and No. one baffle 102 looks vertically spawning board 105 the lateral wall of end box 101 evenly is equipped with a plurality of release holes 106 it is equipped with storage grain box 107 to lie in end box 101 inside wall in the growth zone be equipped with a plurality of No. two baffles 112 that are parallel to each other in the storage grain box 107, the inside rice moth fodder that is equipped with of storage grain box 107 the symmetry is equipped with two racks 113 about the inside wall lower part of storage grain box 107, every both ends all are equipped with dog 115 around the rack 113 store up the bottom plate left and right sides of grain box 107 and be connected with two gears 114 through the transmission, rack 113 matches with gear 114 and uses.
A recovery bag 108 is arranged at the bottom of the bottom box 101.
A connecting rod 301 is arranged at the center of the top cover 3, circular rings 302 are arranged on the upper and lower parts of the connecting rod 301, connecting ropes 303 are arranged on the circular rings 302, and egg cards 304 are tied on the lower parts of the connecting ropes 303.
The control device 4 comprises a solar cell panel 401, the solar cell panel 401 is fixed on the top cover 3, a storage battery 402, a temperature sensing probe 403, a heating rod 404 and a central controller 405 are fixed on the inner wall of the top cover 3, and the solar cell panel 401, the storage battery 402, the temperature sensing probe 403 and the heating rod 404 are electrically connected with the central controller 405.
The lower edge of the top cover 3 is provided with a defense ring 5, and the lower part of the top cover 3 is provided with an insect prevention ring 6 between the defense ring 5 and the connecting ring 2.
The bottom box 101 is in threaded connection with the connecting ring 2, and the connecting ring 2 is in threaded connection with the top cover 3.
The bottom box 101 is made of transparent materials, so that later-stage observation and maintenance are facilitated, the inner wall of a growth area in the bottom box 101 is smooth, rice moth larvae can be prevented from climbing and escaping, the inner wall of a spawning area is rough, and the rice moth larvae after incubation climb and fall back to the growth area; the top cover 3 is made of opaque materials and prevents sunlight from being exposed to the sun; the insect-proof ring 6 is made of glass material and prevents predatory animals from entering.
A method for stabilizing the number of trichogramma of corn borer in field comprises the following steps:
step 1, investigating the insect situation of corn borers: after the average temperature is higher than 5 ℃ every spring day every year, investigating the growth and development state of the corn borers in the corn field every seven days, and when the pupation rate of the corn borers in the overwintering generation reaches 5-10% and the lowest temperature in the field reaches 5-10 ℃, preparing to install a device for stabilizing the quantity of the corn borers trichogramma in the field;
step 2, preparing a rice moth population: respectively placing rice moth eggs, second-instar rice moth larvae, fourth-instar rice moth larvae and adult rice moth into a growth area, respectively placing rice moth feed particles into a grain storage box and the growth area, coating an insect repellent on an insect prevention ring while hot, and coating a sex attractant on an egg laying plate;
step 3, trichogramma population preparation: hanging an egg card carrying a parasitic egg of a fresh corn borer trichogramma and an egg card carrying a parasitic egg of an aged corn borer trichogramma above a growing area, and hanging an egg card carrying a parasitic egg of a fresh pine moth trichogramma and an egg card carrying a parasitic egg of an aged pine moth trichogramma above the growing area;
and 4, mounting the device: suspending a device for stabilizing the quantity of the corn borers trichogramma in the field in a shading area around the corn field according to the distance of 15-20 m and the vertical height of 0.5-2.5 m from the ground, and avoiding direct solarization by sunlight;
step 5, development and oviposition of rice moths: the method comprises the following steps of (1) enabling rice moth adults to lay eggs on a spawning plate, enabling rice moth eggs, second-instar rice moth larvae and fourth-instar rice moth larvae to grow and mature in a growth area in sequence, enabling the rice moth eggs, the second-instar rice moth larvae and the fourth-instar rice moth larvae to lay eggs on the spawning plate in sequence, so that the number of rice moth populations and the number of rice moth eggs in a device for stabilizing the number of corn borer trichogramma in a field can be maintained, and sufficient host eggs are prepared for the parasitic spawning of the corn borer trichogramma;
step 6, hatching, breeding and releasing trichogramma dendrolimi: the development starting temperature of the trichogramma dendrolimi is more than 5 ℃ and is earlier than the egg development of the trichogramma dendrolimi; the method comprises the following steps of (1) enabling parasitic eggs of aged trichogramma dendrolimi on egg cards and parasitic eggs of fresh trichogramma dendrolimi to successively grow into adult trichogramma dendrolimi, biting egg shells from the parasitic eggs, enabling the trichogramma dendrolimi to fly to an egg laying plate immediately after the trichogramma dendrolimi emerges from the shells, searching fresh eggs of the trichogramma dendrolimi on the egg laying plate, puncturing the fresh eggs of the trichogramma dendrolimi by using a tail egg laying device, laying a new generation of trichogramma dendrolimi eggs in the eggs, realizing passage of the trichogramma dendrolimi trichodermalis in a device for stabilizing the number of the trichogramma dendrolimi, enabling part of the trichogramma dendrolimi trichogramma which does not find suitable fresh eggs to be separated from the device for stabilizing the number of the trichogramma dendrolimi;
step 7, hatching, breeding and releasing the corn borers trichogramma: the development starting temperature of the corn borer trichogramma is more than 12 ℃, and the development is later than that of eggs of the pine moth trichogramma; the parasitic eggs of the old corn borer trichogramma on the egg cards and the parasitic eggs of the fresh corn borer trichogramma develop into the corn borer trichogramma imagoes in sequence, the egg shells are bitten through the parasitic eggs, the corn borer trichogramma flies to the egg laying plate immediately after emerging from the shells, the fresh rice borer eggs are searched on the egg laying plate, the fresh rice borer eggs are punctured by a tail egg laying device, new generation of corn borer trichogramma eggs are laid in the fresh rice borer eggs, passage of the corn borer trichogramma in a device for stabilizing the number of the field corn borer trichogramma is realized, part of the corn borer trichogramma which does not find the appropriate fresh rice borer eggs are separated from the device for stabilizing the number of the corn borer trichogramma through release holes, and the appropriate eggs of the corn borer are searched in the field as hosts.
The ratio of the rice moth eggs, the second-instar rice moth larvae, the fourth-instar rice moth larvae and the adult rice moth in the step 2 to the growth area is 20:2:2: 1; in the step 3, the number ratio of the fresh corn borer trichogramma to the aged corn borer trichogramma is 1:2, and the number ratio of the fresh pine moth trichogramma to the aged pine moth trichogramma is 2: 1.
The preparation method of the rice moth feed particles in the step 2 comprises the following steps:
step 1.1, according to the weight ratio: 9: 1, mixing and powdering coarse grains and fine grains according to the proportion to prepare rice moth feed powder; wherein the coarse grain is one or a mixture of more than two of rice bran, soybean meal, aged wheat, aged rice and aged corn in any proportion; the fine grain is one or a mixture of more than two of brown sugar powder, yellow mealworm powder and fish meal in any proportion;
step 1.2, after the rice moth feed powder is sieved by a 100-mesh sieve, dry heat sterilization is carried out for 30 minutes at 150 ℃, worm eggs, mould and germs in the rice moth feed powder are sterilized and inactivated, the rice moth feed powder is cooled to room temperature, and extrusion granulation is carried out to form rice moth feed particles with the diameter of 5-10 mm;
the preparation method of the insect repellent in the step 2 comprises the following steps:
selecting 3 parts of quercetin, 1.5 parts of xanthotoxin, 3 parts of 2-tridecanone and 3 parts of eugenol, adding 200 parts of 95 ℃ hot ethanol, and uniformly stirring to finish the preparation of the insect repellant;
the preparation method of the neutral attractant in the step 2 comprises the following steps:
step 2.1, mixing 15 parts of male rice moth with 40 parts of water and 55 parts of ethanol, manually homogenizing for 10 minutes by using a glass homogenizer, centrifuging for 10 minutes at 15000 r/g, taking supernatant, centrifuging for 10 minutes at 15000 r/g, and taking supernatant to obtain a crude rice moth extract;
and 2.2, mixing and stirring 100 parts of the crude extract of the rice moth with 1 part of tetradecenol acetate, 1 part of tetradecenol, 1 part of hexadecenol acetate, 1 part of hexadecenol alcohol and 100 parts of acetone uniformly to finish the preparation of the sex attractant.
The preparation method of the insect repellent in the step 2 in the present example is:
or selecting 7 parts of quercetin, 3.5 parts of xanthotoxin, 5 parts of 2-tridecanone and 9 parts of eugenol, adding 300 parts of 95 ℃ hot ethanol, and stirring uniformly to complete the preparation of the insect repellant.
Or 5 parts of quercetin, 2 parts of xanthotoxin, 4 parts of 2-tridecanone and 6 parts of eugenol are selected, 250 parts of 95 ℃ hot ethanol is added, and the mixture is uniformly stirred, so that the preparation of the insect repellent is completed.
The bee storage device 1, the bottom box 101, the first partition plate 102, the spawning plate 105, the release hole 106, the grain storage box 107, the recovery bag 108, the waste hole 109, the fan-shaped baffle plate 110, the rotating shaft 111, the second partition plate 112, the rack 113, the gear 114, the stopper 115, the connecting ring 2, the top cover 3, the connecting rod 301, the circular ring 302, the connecting rope 303, the egg card 304, the control device 4, the solar cell panel 401, the storage battery 402, the temperature sensing probe 403, the heating rod 404, the central controller 405, the defense ring 5 and the insect prevention ring 6 are all universal standard parts or parts known by a person skilled in the art, and the structure and the principle of the invention are known by the person skilled in the art through technical manuals or conventional experimental methods.
While there have been shown and described what are at present considered the fundamental principles and essential features of the invention and its advantages, it will be apparent to those skilled in the art that the invention is not limited to the details of the foregoing exemplary embodiments, but is capable of other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims (10)

1. The utility model provides a stabilize device of field corn borer trichogrammatid quantity which characterized in that: including storing up bee device (1), go-between (2), top cap (3) and controlling means (4), store up bee device (1) and go-between (2) fixed connection, go-between (2) and top cap (3) fixed connection, controlling means (4) set up on the top cap, store up bee device (1) including end box (101), end box (101) and go-between (2) fixed connection, the vertical baffle (102) that is equipped with in middle part in end box (101), baffle (102) divide into two cavities of growing zone and spawning zone with end box (101), all be equipped with waste hole (109) on growing zone and the spawning zone bottom plate waste hole (109) lie in growing zone and spawning zone bottom plate lower terminal surface symmetry and be equipped with sector baffle (110), sector baffle (110) are through the rotatable setting of pivot (111) on material hole (109), the rotating shaft (111) is in transmission connection with an output shaft of a motor, a plurality of spawning plates (105) vertical to the first clapboard (102) are arranged on the side panel of the first clapboard (102) in the spawning area, a plurality of release holes (106) are uniformly arranged on the side wall of the bottom box (101), a grain storage box (107) is arranged on the inner side wall of the bottom box (101) in the growth area, a plurality of second parallel clapboards (112) are arranged in the grain storage box (107), rice moth feed is filled in the grain storage box (107), two racks (113) are symmetrically arranged at the lower parts of the left and right inner side walls of the grain storage box (107), the front end and the rear end of each rack (113) are respectively provided with a stop block (115), the left side and the right side of the bottom plate of the grain storage box (107) are connected with two gears (114) through transmission devices in a transmission way, and the racks (113) are matched with the gears (114) for use.
2. The device of claim 1, wherein the device comprises: a recovery bag (108) is arranged at the bottom of the bottom box (101).
3. The device of claim 1, wherein the device comprises: the top center of top cap (3) is equipped with connecting rod (301), connecting rod (301) upper and lower part all is equipped with ring (302) be equipped with on ring (302) connect rope (303), in the lower part connect rope (303) go up to tie down egg card (304).
4. The device of claim 1, wherein the device comprises: the control device (4) comprises a solar cell panel (401), the solar cell panel (401) is fixed on the top cover (3), a storage battery (402), a temperature sensing probe (403), a heating rod (404) and a central controller (405) are fixedly arranged on the inner wall of the top cover (3), and the solar cell panel (401), the storage battery (402), the temperature sensing probe (403) and the heating rod (404) are electrically connected with the central controller (405).
5. The device of claim 1, wherein the device comprises: the lower edge of the top cover (3) is provided with a defense ring (5), and the lower part of the top cover (3) is provided with an insect prevention ring (6) between the defense ring (5) and the connecting ring (2).
6. The device of claim 3, wherein the device comprises: the bottom box (101) is in threaded connection with the connecting ring (2), and the connecting ring (2) is in threaded connection with the top cover (3).
7. The device of claim 5, wherein the device comprises: the bottom box (101) is made of transparent materials, so that later-stage observation and maintenance are facilitated, the inner wall of a growth area in the bottom box (101) is smooth, rice moth larvae can be prevented from climbing and escaping, the inner wall of a spawning area is rough, and the incubated rice moth larvae climb and fall back to the growth area; the top cover (3) is made of opaque materials and prevents sunlight from being exposed to the sun; the insect-proof ring (6) is made of glass materials and prevents predatory animals from entering.
8. A method for stabilizing the number of trichogramma of corn borer in field is characterized in that: the method comprises the following steps:
step 1, investigating the insect situation of corn borers: after the average temperature is higher than 5 ℃ every spring day every year, investigating the growth and development state of the corn borers in the corn field every seven days, and when the pupation rate of the corn borers in the overwintering generation reaches 5-10% and the lowest temperature in the field reaches 5-10 ℃, preparing to install a device for stabilizing the quantity of the corn borers trichogramma in the field;
step 2, preparing a rice moth population: respectively placing rice moth eggs, second-instar rice moth larvae, fourth-instar rice moth larvae and adult rice moth into a growth area, respectively placing rice moth feed particles into a grain storage box and the growth area, coating an insect repellent on an insect prevention ring while hot, and coating a sex attractant on an egg laying plate;
step 3, trichogramma population preparation: hanging an egg card carrying a parasitic egg of a fresh corn borer trichogramma and an egg card carrying a parasitic egg of an aged corn borer trichogramma above a growing area, and hanging an egg card carrying a parasitic egg of a fresh pine moth trichogramma and an egg card carrying a parasitic egg of an aged pine moth trichogramma above the growing area;
and 4, mounting the device: suspending a device for stabilizing the quantity of the corn borers trichogramma in the field in a shading area around the corn field according to the distance of 15-20 m and the vertical height of 0.5-2.5 m from the ground, and avoiding direct solarization by sunlight;
step 5, development and oviposition of rice moths: the method comprises the following steps of (1) enabling rice moth adults to lay eggs on a spawning plate, enabling rice moth eggs, second-instar rice moth larvae and fourth-instar rice moth larvae to grow and mature in a growth area in sequence, enabling the rice moth eggs, the second-instar rice moth larvae and the fourth-instar rice moth larvae to lay eggs on the spawning plate in sequence, so that the number of rice moth populations and the number of rice moth eggs in a device for stabilizing the number of corn borer trichogramma in a field can be maintained, and sufficient host eggs are prepared for the parasitic spawning of the corn borer trichogramma;
step 6, hatching, breeding and releasing trichogramma dendrolimi: the development starting temperature of the trichogramma dendrolimi is more than 5 ℃ and is earlier than the egg development of the trichogramma dendrolimi; the method comprises the following steps of (1) enabling parasitic eggs of aged trichogramma dendrolimi on egg cards and parasitic eggs of fresh trichogramma dendrolimi to successively grow into adult trichogramma dendrolimi, biting egg shells from the parasitic eggs, enabling the trichogramma dendrolimi to fly to an egg laying plate immediately after the trichogramma dendrolimi emerges from the shells, searching fresh eggs of the trichogramma dendrolimi on the egg laying plate, puncturing the fresh eggs of the trichogramma dendrolimi by using a tail egg laying device, laying a new generation of trichogramma dendrolimi eggs in the eggs, realizing passage of the trichogramma dendrolimi trichodermalis in a device for stabilizing the number of the trichogramma dendrolimi, enabling part of the trichogramma dendrolimi trichogramma which does not find suitable fresh eggs to be separated from the device for stabilizing the number of the trichogramma dendrolimi;
step 7, hatching, breeding and releasing the corn borers trichogramma: the development starting temperature of the corn borer trichogramma is more than 12 ℃, and the development is later than that of eggs of the pine moth trichogramma; the parasitic eggs of the old corn borer trichogramma on the egg cards and the parasitic eggs of the fresh corn borer trichogramma develop into the corn borer trichogramma imagoes in sequence, the egg shells are bitten through the parasitic eggs, the corn borer trichogramma flies to the egg laying plate immediately after emerging from the shells, the fresh rice borer eggs are searched on the egg laying plate, the fresh rice borer eggs are punctured by a tail egg laying device, new generation of corn borer trichogramma eggs are laid in the fresh rice borer eggs, passage of the corn borer trichogramma in a device for stabilizing the number of the field corn borer trichogramma is realized, part of the corn borer trichogramma which does not find the appropriate fresh rice borer eggs are separated from the device for stabilizing the number of the corn borer trichogramma through release holes, and the appropriate eggs of the corn borer are searched in the field as hosts.
9. The method for stabilizing the number of trichogramma of the corn borer in the field according to claim 8, wherein the method comprises the following steps: the ratio of the rice moth eggs, the second-instar rice moth larvae, the fourth-instar rice moth larvae and the adult rice moth in the step 2 to the growth area is 20:2:2: 1; in the step 3, the number ratio of the fresh corn borer trichogramma to the aged corn borer trichogramma is 1:2, and the number ratio of the fresh pine moth trichogramma to the aged pine moth trichogramma is 2: 1.
10. The method for stabilizing the number of trichogramma of the corn borer in the field according to claim 8, wherein the method comprises the following steps:
the preparation method of the rice moth feed particles in the step 2 comprises the following steps:
step 1.1, according to the weight ratio: 9: 1, mixing and powdering coarse grains and fine grains according to the proportion to prepare rice moth feed powder; wherein the coarse grain is one or a mixture of more than two of rice bran, soybean meal, aged wheat, aged rice and aged corn in any proportion; the fine grain is one or a mixture of more than two of brown sugar powder, yellow mealworm powder and fish meal in any proportion;
step 1.2, after the rice moth feed powder is sieved by a 100-mesh sieve, dry heat sterilization is carried out for 30 minutes at 150 ℃, worm eggs, mould and germs in the rice moth feed powder are sterilized and inactivated, the rice moth feed powder is cooled to room temperature, and extrusion granulation is carried out to form rice moth feed particles with the diameter of 5-10 mm;
the preparation method of the insect repellent in the step 2 comprises the following steps:
selecting 3-7 parts of quercetin, 1.5-3.5 parts of xanthotoxin, 3-5 parts of 2-tridecanone and 3-9 parts of eugenol, adding 200-300 parts of 95 ℃ hot ethanol, and uniformly stirring to finish the preparation of the insect repellent;
the preparation method of the neutral attractant in the step 2 comprises the following steps:
step 2.1, mixing 15 parts of male rice moth with 40 parts of water and 55 parts of ethanol, manually homogenizing for 10 minutes by using a glass homogenizer, centrifuging for 10 minutes at 15000 r/g, taking supernatant, centrifuging for 10 minutes at 15000 r/g, and taking supernatant to obtain a crude rice moth extract;
and 2.2, mixing and stirring 100 parts of the crude extract of the rice moth with 1 part of tetradecenol acetate, 1 part of tetradecenol, 1 part of hexadecenol acetate, 1 part of hexadecenol alcohol and 100 parts of acetone uniformly to finish the preparation of the sex attractant.
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CN112889762A (en) * 2021-03-25 2021-06-04 贵州卓豪农业科技股份有限公司 Portable trichogramma purification rejuvenation breeding device
CN114342884B (en) * 2022-02-24 2022-11-29 黑龙江省农业科学院 Trichogrammatid bee card fixing device

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