WO2012000174A1

WO2012000174A1 - Method for breeding fly maggots with medicinal value

Info

Publication number: WO2012000174A1
Application number: PCT/CN2010/074717
Authority: WO
Inventors: 陈建平
Original assignee: 周星伟
Priority date: 2010-06-29
Filing date: 2010-06-29
Publication date: 2012-01-05

Abstract

A method for breeding fly maggots with medicinal value comprises breeding adult flies and breeding fly maggots. An adult fly house is made of mesh cloth and thin plastic film. The top of the adult fly house is made of glass steel tile. The adult fly house is provided with ropes and dishes for collecting flyblows. The top of a fly maggot house is made of black adumbral mesh cloth. The fly maggot house is provided with a maggot breeding pond and a maggot collecting pond. The feed for the adult flies is composed of milk powder (80-95 weigh parts), brown sugar (1-3 weigh parts) and fruits (10-15 weigh parts). The feed for the fly maggots is composed of animal protein (25-100 weigh parts), vegetables (0-30 weigh parts) and anticancer factors (3-5 weigh parts). The animal protein is at least two combinations comprising frogs, freshwater fishes and animal viscera. The anticancer factors are any one or at least two combinations comprising yew leaf, stellera chamaejasme, and nidus vespae. The fly maggots bred by the method have a quite good inhibiting effect on the growth of multiple tumors and bacteria, and their antineoplastic biological activity is between 80 to 97 percent.

Description

Breeding method with medicinal value maggots

The invention relates to the technical field of breeding and breeding, and more particularly to a method for breeding maggots with medicinal value. Background technique

The breeding ability of flies is in the forefront of insects. The larvae of flies are called fly maggots, which grow very fast. The maggot protein is a high-quality protein. After special processing, antibacterial active protein, compound amino acid and emu oil can be extracted from maggots. , such as chitin, not only high-quality feed, but also protein powder, high-grade nutritional products, aerospace foods, medicines, etc., so it has a wide range of uses in medicine, health care products, chemicals and other fields. The artificial breeding of flies originated in the late 1970s and early 1980s. Although the fly maggot culture has been proposed for more than 20 years, it has not yet reached the scale. In the rural areas, the cultivation in the countryside cannot be warmed up. The level of science and technology is relevant, and the high-efficiency aseptic cage method just makes up for this. The high-efficiency aseptic cage technology is popular and easy to understand. It is suitable for both small-scale farms and larger farms and aquaculture farms. However, the most direct use of the current breeding of maggots is still to serve as a feed and cannot meet the needs of health foods and medicinal products.

The Chinese Patent Office has disclosed a large-scale breeding method and equipment for maggots. The bulletin number is CN1149013C. This document discloses a large-scale breeding method and equipment for maggots. The fly-flying equipment for large-scale breeding of maggots is closed by breeding. Box, feather tray, connecting cloth bag, plastic sheet for feeding, plastic sheet for collecting eggs, sponge for drinking water; larval culture equipment consists of larval incubator and larval culture room; seed mash feed according to designed nutrients, proportion and process , fly bait and commodities 蛆 feeding; breeding, management of fly, adult and commercial cockroaches according to design scale and production process. However, the fly maggot cultivated by this method is only for providing a feed protein and an edible protein, and cannot meet the demand for medicinal use. Summary of the invention

At present, the artificial breeding method of maggots cannot meet the needs of health foods and medicinal materials, and in order to solve the deficiencies in the prior art, the present invention proposes a breeding method with medicinal value of maggots.

The object of the invention is achieved by the following technical solution:

A method of breeding medicinal value of maggots, including adult fly breeding and fly maggot breeding, and the breeding method comprises the following steps:

(1) Adult fly breeding: The fly is placed in the fly-flying room. The fly-fly room is a translucent fly house surrounded by a glass fiber reinforced plastic roof and surrounded by a nylon mesh. The fly-fly room has a rope and The egg pots are placed in the egg pots, and the animal protein is used for attracting the eggs to concentrate on spawning. The animal protein is a mixture of fish paste or mash or a mixture of the two in any proportion; the fly feed is prepared in the following parts by weight: 80 to 95 parts of milk powder, 1 to 3 parts of brown sugar, 10 to 15 parts of fruit;

(2) Fly maggot breeding: The eggs produced by the adult flies are placed in the maggot chamber, and the roof of the maggot chamber is covered with black shade net material, and the maggot chamber has a pond and a collecting pond; The feed is composed of 25 to 100 parts of animal protein, 0 to 30 parts of vegetables, and 3 to 5 parts of anti-cancer factors, wherein the animal protein is a combination of two or more of frog, freshwater fish and animal internal organs. The anticancer factor is any one or a combination of two or more of the yew leaf, the wolfberry venom or the apiary.

The adult fly uses Chrysomyia megacephala as a species fly, which has the characteristics of fast reproduction, strong disease resistance and high temperature resistance, so that it does not degenerate to ensure efficacy. Since this species is a special fly species, it is not required. "Proovin", its egg-laying quantity can fully meet the design requirements, and the eggs cultivated by the culture method using the invention grow up as a species fly, and the medicinal value is more obvious. Preferably, the fly-forming room is 10 to 20 cubic meters, and the feeding amount is 100,000 to 200,000 adult flies, the temperature is controlled within the range of 28 ° C to 35 ° C, and the adult fly house is hung 8 to 20 rope. The fly-flying room hangs the rope for the fly to rest. The number of ropes to be hung depends on the size of the house. The "big-headed gold fly" is characterized by livelihood, active, not easy to raise, high feeding requirements, and comparison of temperature conditions. high.

Preferably, a feeding pond is placed every 1.5 to 2 square meters in the maggot chamber, and the temperature of the feeding pond is controlled within a range of 30 ° C to 40 ° C. This ventilation condition is good, and the pond has the advantages of acid removal, firmness and gas permeability.

Preferably, the oyster pool is made of glue, wood slag, steel bar and plywood. The glue is made of light burnt magnesium powder mixed with magnesium chloride and water to form a gel, wherein the light burnt magnesium powder and magnesium chloride are used. The weight ratio is 15~25: 75~85. The wood slag is made of odorless wood residue, and the plywood is also made of odorless material. The wood slag, the round steel bar and the plywood are bonded by the light-burning magnesium powder and magnesium chloride which are adjusted into glue. The setting of the pond is conducive to the growth of maggots and facilitates the management of maggots.

Preferably, a light source is provided on the pond. The light source may be a lighting device such as an electric lamp. The invention utilizes the characteristics of the long fly maggots to protect the light from the front, and adopts the light collecting method to turn on the light, and most of the maggots will automatically climb away from the bait to the darker. Close to the pool, unhealthy cockroaches, dead cockroaches and debris and natural and healthy cockroaches are naturally separated, facilitating the collection of maggots.

Preferably, the freshwater fish is salmon, squid, squid or grass carp, the animal viscera is porcine lung, and the vegetable is any one or a combination of two or more of cabbage, tomato or cucumber.

Preferably, the fly maggot feed comprises, by weight: 15 to 80 parts of frog, 10 to 30 parts of freshwater fish, 5 to 8 parts of tomato, 5 to 8 parts of cabbage, 3 to 4 parts of yew leaf, and 0.3 to 0.6 of wolfberry poison Share.

Preferably, the fly maggot feed comprises, in parts by weight: 15 to 80 parts of pig lungs, 10 to 30 parts of freshwater fish, 5 to 8 parts of tomatoes, 5 to 8 parts of cabbage, 3 to 4 parts of yew leaves, and 0.3 to dew. 0.6 parts. Preferably, the fly maggot feed comprises, in parts by weight: 30 to 50 parts of the frog, 30 to 50 parts of the pig lung, 5 to 20 parts of the cabbage, 2.4 to 4 parts of the yew leaf, and 0.6 to 1 part of the apiary.

Preferably, the fly maggot feed comprises, by weight: 15 to 35 parts of frog, 15 to 35 parts of pig lung, 10 to 30 parts of freshwater fish, 3 to 5 parts of tomato, 3 to 5 parts of cucumber, 3 to 5 parts of cabbage, 2 to 4 parts of yew leaves, 0.2 to 0.3 parts of wolf venom, and 0.6 to 1 part of dew.

The above feed can be fed freshly after being processed slightly. After several years of testing by the National New Drug Screening Center, the above-mentioned feeder-fed maggots have a strong inhibition rate on various tumors and bacterial growth, and their anti-tumor biological activities are 80%-97%.

Compared with the prior art, the beneficial effects of the present invention are: The medicinal value of the fly maggot cultured according to the present invention has been tested by the National New Drug Screening Center and related departments for several years, and has strong growth for various tumors and bacteria. The inhibition rate, its anti-tumor biological activity is between 80% and 97%. detailed description

The invention selects "Golden-headed Golden Fly" as the fly species, "Chrysomyia megacephala". Because the fly species is a special fly species, it does not need "oval", and its egg-laying quantity can fully meet the design requirements. At the same time, the egg cultivated by the culture method of the present invention grows up as a species fly, and the medicinal value is more obvious.

In the environment without pollution, good water quality, relatively independent environment built to fly house and fly maggots. (1) Adult fly breeding: The fly is raised in the fly-flying room. The roof of the fly-flying house is constructed of glass-steel tiles. The surrounding material is made of 4 cm x 5 cm of wood and is semi-transparent with nylon mesh. Fly room, each room is 3 meters x 2 meters X 2. 5 meters, the breeding capacity is 100,000 ~ 200,000 species of flies, the room temperature is controlled within the range of 28 ° C ~ 35 ° C, 10 in the room The rope is for the fly to rest. In the adult fly house, a set of egg pots is placed in the egg pot, and the animal protein for attracting the eggs to concentrate on spawning is placed in the egg pot. The animal protein is Fish paste or mash or a mixture of the two in any ratio; adult fly words: milk powder 80~95 grams, brown sugar 1~3 grams, fruit 10~15 grams, and the right amount of water.

(2) Fly maggot breeding: The eggs produced by the adult flies are placed in the maggot chamber, and the roof of the maggot chamber is covered with black shade net material. The fly maggot house has a pond and a collecting pond, and the fly A potting pond is placed every 1.5~2 square meters in the boudoir, and the temperature of the pond is controlled within the range of 30 °C ~ 40 °C.

The oyster pool is made of glue, wood slag, steel bar and plywood. The size is 0. 7 m X 0. 5 m X 0. 6 m. The glue is made of light burnt magnesium powder mixed with magnesium chloride and water to form a gel. The weight ratio of light burned magnesium powder to magnesium chloride is 15~25: 75~85. The wood slag is made of odorless wood slag, and the plywood is also made of odorless material. The slag, the round steel bar and the plywood are bonded by the light-burning magnesium powder and magnesium chloride which are adjusted into glue. The pond made by the above method has the advantages of acid removal, firmness and venting. The maggots are placed in the pool, and the fly maggots are placed at the same time. The temperature in the pond is controlled at about 35 degrees.

The fly maggot feed consists of 25 to 100 parts of animal protein, 0 to 30 parts of vegetables and 3 to 5 parts of anti-cancer factors, wherein the animal protein is two or more of frog, freshwater fish and animal internal organs. The combination of the anticancer factor is any one or a combination of two or more of the yew leaf, the wolfberry venom or the apiary. The raw materials of the fly maggot feed are shown in Table 1 in parts by weight, and the various raw materials are pulverized and mixed to form fly maggot feed.

There is also a 2m x 4m X 0. 25m receiving pool in the fly maggot room. Using the characteristics of the long fly maggots to prevent light before use, the light collection method will be used to lighten the light. The collection pool is close, and unhealthy cockroaches, dead cockroaches and debris and natural and healthy cockroaches are naturally separated.

Table 1 Formulas of fly maggots of Examples 1 to 12 (unit: kilograms)

Frog 15 80 35 15 30 50 40 30 100 Pig lung 35 15 50 30 15 80 30 50 100 Salmon 30 Salmon 20 10 Salmon 15 30 10 Grass fish 30 10 10 Tomato 8 5 3 5 5 8 10 10 3 Cucumber 3 5 10 10 3 Cabbage 5 8 5 3 5 20 8 5 10 10 3 Yew

3 4 4 2 2. 4 4 3 4 4. 5 2. 7 4 2. 7 Leaf wolf venom 0. 6 0. 3 0. 3 0. 2 0. 2 0. 1 0. 1 0. 1 6 1 1 0. 6 0. 3 0. 6 0. 3 0. 2 1 0. 2

Application of medicinal value of maggots: The collected maggots are rinsed to remove ineffective debris, then frozen, thawed, sterilized, rinsed, and dried and powdered at a temperature of 40 ° C to 45 ° C. The powder can be eaten directly and can achieve anti-cancer effects without any separation. The fly maggots obtained from the above examples were made into powder according to the above method, and were tested by the National New Drug Screening Center for a strong inhibition rate on various tumors and bacterial growth, and the antitumor biological activity was 80°/. ~97%, the specific test results are as follows: 1. National New Drug Screening Center

Nat iona l Cent er for Drug Screening Shanghai, China

Anti-tumor biological activity in vitro screening experiment

Filter by: sulforhodamine B, SRB

Protein staining

Fine plant: KB human squamous cell carcinoma

Duration: 72 hours

The inhibition rate of tumor fine packet growth is /.

KB concentration (mg/ml) 1 0.25 0.063 0.016 0.0039 Rating Remarks Sample number

115426 95.7 97.9 0 0 0 Active

Date: 2003-03-07

2. National New Drug Screening Center

National Center for Drug Screening

Shanghai, China

Anti-tumor biological activity in vitro screening experiment

Filter method: sulforhodamine B, SRB

Protein staining

Fine package forest: BEL-7402 human liver cancer Duration: 72 hours

% inhibition of tumor fine bag growth

BEL-7402 Human liver cancer concentration (mg/ml) 1 0.25 0.063 0.016 0.0039 Reviews Remarks Sample number

115426 88.5 95.4 20.1 4.1 2.3 Active

115427 92.1 70.6 13.0 2.5 6.1 Active

Date: 2003-03-07

3. National New Drug Screening Center

National Center for Drug Screening

Shanghai, China

Anti-tumor biological activity in vitro screening experiment

Filter method: sulforhodamine B, SRB

Protein staining

Cell line: 11 (^-116 human colon cancer)

Duration: 72 hours

% inhibition of tumor cell growth

HCT-116 human colon cancer Concentration (mg/ml) 1 0. 25 0. 063 0. 016 0. 0039 Evaluation Remarks Sample Number

115426 93.9 83.6 0 0 0 Active

Date: 2003-05-23

4. National New Drug Screening Center

Nat iona l Cent er for Drug Screening

Shangha i , China

Anti-tumor biological activity in vitro screening experiment

Selection method: Tetrazolium salt (Methyl-Thiazol-Tetrozol ium, MTT)

Reduction method

Cell line: HL- 60 human leukemia

Duration: 72 hours

The inhibition rate of tumor cell growth is ⁰ /.

Concentration (mg/ml) 1 0. 25 0. 063 0. 016 0. 004 Evaluation Remarks Sample number

NC00122000 87.9 100 13.1 6.3 2.2 Active

Date: 2005-10-25

5. National New Drug Screening Center National Center for Drug Screening

Shanghai, China

Anti-tumor biological activity in vitro screening experiment

Selection method: Tetrazolium salt (Methyl-Thiazol-Tetrozol ium, MTT)

Reduction method

Cell Forest: P-388 Mouse Leukemia

Duration: 72 hours

The inhibition rate of tumor cell growth is ⁰ /.

Concentration (mg/ml) 1 0.25 0.063 0.016 0.004 Rating Remarks Sample number

NC00122000 86.3 99.8 2.0 0 0 Active

Date: 2005-10-25

6. National New Drug Screening Center

National Center for Drug Screening

Shanghai, China

Anti-tumor biological activity in vitro screening experiment

Filter method: Tetrazolium salt (Methy Pu Thiazol- Tetrozol ium, MTT )

Reduction method

Fine sputum strain: A- 549 human sputum cancer

Duration: 72 hours % inhibition of tumor cell growth

Concentration (mg/ml) 1 0.25 0.063 0.016 0.0039 Rating Remarks Sample number

NC00122000 95.9 97.3 13.4 0 1.6 Active Date: 2005

7. National New Drug Screening Center

National Center for Drug Screening

Shanghai, China

Anti-tumor biological activity in vitro screening experiment

Filter method: sulforhodamine B, SRB

Protein staining

Fine Baolin: BEL-7402 Human Liver Cancer

Duration: 72 hours

The inhibition rate of tumor cell growth is ⁰ /.

Concentration (mg/ml) 1 0.25 0.063 0.016 0.0039 Rating Remarks Sample number

NC00122000 93.7 97.1 2.6 0 0 Active

Date: 2005-10-25 8,

Antibacterial effect test report Screening model: Antibacterial activity

Test system: in vitro

Determination method: micro liquid dilution method, agar dilution method, judged by minimum inhibitory concentration (MIC) value and minimum bactericidal concentration (MBC) value

Evaluation of results: MIC value 20ug/ml is valid

MIC (ug/ml)

Sample No. Aspergillus white Candida albicans Saccharomyces Cerevisiae Remarks

NC00072301 >100 >100 >100

NC00072302 >100 >100 >100

Sample number Pityrosporum Red hair fungus Gypsum-like fungus

NC00072301 >100 >100 >100

NC00072302 >100 >100 >100

MIC (ug/ml)

Sample No. Staphylococcus aureus Staphylococcus aureus Streptococcus mutans Remarks NC00072303 >100 >100 >100 >100

NC00072304 >100 >100 >100 >100 Sample No. S. aureus Escherichia coli

NC00072303 >100 >100 >100

NC00072304 >100 >100 >100 Note: The sample is not well water-soluble, and the supernatant is tested during the experiment.

Report date: October 9, 2004

Claims

Rights request

A method of breeding a medicinal value of maggots, comprising adult fly breeding and fly maggot breeding, characterized in that the farming method comprises the following steps:

(1) Adult fly breeding: The fly is placed in the fly-flying room. The fly-fly room is a translucent fly house surrounded by a glass fiber reinforced plastic roof and surrounded by nylon mesh. The fly-fly room has a rope and The egg-pots are placed in the egg-pots, and the animal protein is used to induce the eggs to concentrate on spawning. The animal protein is a mixture of fish paste or mash or a mixture of the two in any proportion; the fly feed is prepared in the following parts by weight: milk powder 80 to 95 parts, 1 to 3 parts of brown sugar, 10 to 15 parts of fruit;

(2) The words of the fly maggots: The eggs produced by the adult flies are placed in the maggot house, and the roof of the maggot house is covered with black shade net material, and the fly maggot house has a pond and a collecting pond; The mash feed consists of 25 to 100 parts of animal protein, 0 to 30 parts of vegetables, and 3 to 5 parts of anti-cancer factors, wherein the animal protein is two or more of frogs, freshwater fish, and animal viscera. In combination, the anticancer factor is any one or a combination of two or more of the yew leaf, the steller venom or the apiary.

2. The culture method according to claim 1, wherein the fly-flying is: 10 to 20 cubic meters of the fly-flying house, and the feeding amount is 100,000 to 200,000 adult flies, and the temperature is controlled at 28 In the range of °C~35°C, 8~20 ropes are hung in the fly-flying room.

3. The culture method according to claim 1, wherein a pond is placed in the maggot chamber every 1.5 to 2 square meters, and the temperature of the pond is controlled in the range of 30 ° C to 40 ° C. Inside.

The culture method according to claim 1 or 3, wherein the oyster pool is made of glue, wood slag, steel bars and plywood, and the glue is made of light burnt magnesium powder and The magnesium chloride is mixed with water to form a gel, wherein the weight ratio of the light burnt magnesium powder to the magnesium chloride is 15 to 25: 75 to 85.

5. The culture method according to claim 1 or 3, characterized in that the light source is provided on the pond.

The culture method according to claim 1, wherein the freshwater fish is squid, squid, squid or grass carp, the animal viscera is pig lung, and the vegetable is any one of cabbage, tomato or cucumber. Or a combination of two or more.

The culture method according to claim 1, wherein the fly maggot feed comprises, in parts by weight, 15 to 80 parts of frogs, 10 to 30 parts of freshwater fish, 5 to 8 parts of tomatoes, and 5 to 8 parts of cabbage. 8 parts, 3 to 4 parts of yew leaves, 0.3 to 0.6 parts of wolf venom.

The culture method according to claim 1, wherein the fly maggot feed comprises, in parts by weight, 15 to 80 parts of pig lungs, 10 to 30 parts of freshwater fish, 5 to 8 parts of tomatoes, and cabbage 5 ~ 8 parts, 3 to 4 parts of yew leaves, 0.3 to 0.6 parts of dew.

The culture method according to claim 1, wherein the fly maggot feed comprises, in parts by weight, 30 to 50 parts of frogs, 30 to 50 parts of pig lungs, 5 to 20 parts of cabbage, and yew leaves. 2.4-4 parts, 0.6 to 1 part of the exposed bee.

The culture method according to claim 1, wherein the fly maggot feed comprises, by weight: 15 to 35 parts of frog, 15 to 35 parts of pig lung, 10 to 30 parts of freshwater fish, and tomato 3 ~5 parts, 3 to 5 parts of cucumber, 3 to 5 parts of cabbage, 2 to 4 parts of yew leaves, 0.2 to 0.3 parts of wolfberry, and 0.6 to 1 part of dew.