CN106973862A - A kind of method that fly maggot breeding is carried out using fruit discarded object - Google Patents
A kind of method that fly maggot breeding is carried out using fruit discarded object Download PDFInfo
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- 235000013399 edible fruits Nutrition 0.000 title claims abstract description 55
- 238000000034 method Methods 0.000 title claims abstract description 27
- 238000009395 breeding Methods 0.000 title claims abstract description 15
- 230000001488 breeding effect Effects 0.000 title claims abstract description 15
- 239000001963 growth medium Substances 0.000 claims abstract description 33
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 9
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 9
- 210000004681 ovum Anatomy 0.000 claims abstract description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 8
- 230000008569 process Effects 0.000 claims abstract description 3
- 238000012545 processing Methods 0.000 claims description 18
- 230000001954 sterilising effect Effects 0.000 claims description 15
- 238000004659 sterilization and disinfection Methods 0.000 claims description 15
- 244000144730 Amygdalus persica Species 0.000 claims description 9
- 240000008790 Musa x paradisiaca Species 0.000 claims description 9
- 235000018290 Musa x paradisiaca Nutrition 0.000 claims description 9
- 235000006040 Prunus persica var persica Nutrition 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 7
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 claims description 7
- 235000010234 sodium benzoate Nutrition 0.000 claims description 7
- 239000004299 sodium benzoate Substances 0.000 claims description 7
- 241000257159 Musca domestica Species 0.000 claims description 2
- 238000000498 ball milling Methods 0.000 claims 1
- 238000011081 inoculation Methods 0.000 claims 1
- 239000002994 raw material Substances 0.000 abstract description 3
- 239000004615 ingredient Substances 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 238000004064 recycling Methods 0.000 abstract description 2
- 239000007320 rich medium Substances 0.000 abstract description 2
- 102000004169 proteins and genes Human genes 0.000 description 11
- 108090000623 proteins and genes Proteins 0.000 description 11
- 235000019733 Fish meal Nutrition 0.000 description 9
- 150000001413 amino acids Chemical class 0.000 description 9
- 239000004467 fishmeal Substances 0.000 description 9
- 235000019658 bitter taste Nutrition 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 6
- 239000002002 slurry Substances 0.000 description 6
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- 238000001914 filtration Methods 0.000 description 4
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- 235000019750 Crude protein Nutrition 0.000 description 3
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- 239000004365 Protease Substances 0.000 description 3
- 235000019784 crude fat Nutrition 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
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- 238000011160 research Methods 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- 239000002585 base Substances 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 235000019419 proteases Nutrition 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 239000011701 zinc Substances 0.000 description 2
- 229910052725 zinc Inorganic materials 0.000 description 2
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 238000000944 Soxhlet extraction Methods 0.000 description 1
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
- 229930003471 Vitamin B2 Natural products 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- -1 carbon water Compound Chemical class 0.000 description 1
- ZAIPMKNFIOOWCQ-UEKVPHQBSA-N cephalexin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@@H]3N(C2=O)C(=C(CS3)C)C(O)=O)=CC=CC=C1 ZAIPMKNFIOOWCQ-UEKVPHQBSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000010941 cobalt Substances 0.000 description 1
- 229910017052 cobalt Inorganic materials 0.000 description 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 239000002781 deodorant agent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000001599 direct drying Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000004626 essential fatty acids Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 150000002617 leukotrienes Chemical class 0.000 description 1
- 235000020778 linoleic acid Nutrition 0.000 description 1
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005121 nitriding Methods 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 229940088197 riboflavin 3 mg Drugs 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 235000019164 vitamin B2 Nutrition 0.000 description 1
- 239000011716 vitamin B2 Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a kind of method that fly maggot breeding is carried out using fruit discarded object, this method includes：Fly maggot culture medium prepares, connect the process of ovum and culture；The present invention uses fruit discarded object for primary raw material to prepare fly maggot culture medium first; and carry out the nutritional ingredient of further rich medium with fruit muddy and the suitable ratio collocation of dusty yeast; one aspect of the present invention realizes the recycling of fruit discarded object; protect environment; on the other hand cost also has been saved for fly maggot breeding, has realized resource reasonable disposition utilization.
Description
Technical field
The invention belongs to the cultivation field of fly maggot, and in particular to a kind of side that fly maggot breeding is carried out using fruit discarded object
Method.
Background technology
Fly-maggot protein is because containing chitin, antibacterial skin albumen, alexin, interferon, ectogenous agglutinine and amino acid etc.
It is precious in terms of immunological regulation, toxin expelling, Anti-bacterium are viral and antitumor that unusual active material becomes the mankind
Biological medicine resource.
Zhang Tingjun (1999) reports that fly maggot is containing crude protein 59%~65%, fat 2.6%~12.0%；Compared with fish meal,
Its total amount of essential amino acids, methionine content and lysine content are the 2.3 of fish meal respectively, 2.7 and 2.6 times, wherein required ammonia
Base acid total amount is up to 43.30%, more than world food and the reference value of agricultural organization/World Health Organization (FAO/WHO) proposition
(40%).Plum Ning'an etc. (2002) research shows that crude protein content is 53.4% in fly maggot air dry matter, average higher than domestic fish meal
Level (45.0%), slightly below peru fish meal level (60.4%)；Fly maggot larva air dry matter crude fat is also up to 12.7%, and contains
16 kinds of primary amino acids.Jia Shengfu and Ma Yanbiao (2007) researchs show that fresh maggot contains protein 18.6%, fat 5.0%, carbon water
Compound and salt 3.5%, moisture 71.4%, vitamin B2 (riboflavin 3mg/100g, carrotene 0.4mg/100g)；Dry fly maggot,
The gross protein value of pupa and fly corpse is respectively 60.8%, 58.2% and 64.2%, and crude fat is respectively 17.1%, 14.5% and
6.5%, ash content is respectively 9.2%, 8.1% and 7.5%, the content more than domestic fish meal and soya-bean cake.Si Qingaowa etc.
(2008) fly-maggot protein matter content is measured for 56.18%, and 17 kinds of amino acid contents are higher than dregs of beans and soya-bean cake, close to fish meal, copper,
7 kinds of essential trace element contents such as iron, manganese, zinc, cobalt, selenium, iodine are also higher than imported fish meal.Hu Jinwei etc. (2009) is, it was also found that dry
Dry fly maggot protein content is close with fish meal, and amino acid masses are higher than dregs of beans, close to fish meal.Bai Gang and Zhang Yixiang (2010) researchs
Show, fly maggot crude protein content 62.52%, it is necessary to which amino acid content (tryptophan is not surveyed) accounts for the 47.72% of total amino acid, it is necessary to
The ratio of amino acid and nonessential amino acid total amount is 0.91, is above reference model (40.00%, 0.60)；Crude fat content
18.05%, the essential fatty acid content such as linoleic acid and leukotrienes is respectively 24.21% and 0.68%, unsaturated fatty acid content
58.50%；Total sugar content 2.87%；Vitamin A. D. E content is respectively 727.8,131.0 μ g/100g and 10.04mg/100g；
Fe, Zn, Se, Ca content are respectively 268,159,8.90mg/kg and 0.31%.
In recent years, on the one hand continuing to develop with agricultural science and technology, fruit industry increasing both production and income is the development tribute of economic society
Strength has been offered, on the other hand because being influenceed by environment and market situation, still there are many fruit to become agriculture waste every year
Thing and can not fully be utilized, this both environmental pollution produce destruction, influence or generate very big economic loss；Existing skill
Do not occur also on developing the report of fly maggot culture medium with fruit discarded object in art, if preparing Gao Ying with fruit discarded object
Nourish one's nature fly maggot culture medium, on the one hand will be the reasonable using open up a new way of fruit discarded object, and also be on the other hand training
The fly maggot for supporting high protein saves cost.
The content of the invention
It is an object of the invention to provide a kind of cultural method of fly maggot, be related specifically to it is a kind of using fruit discarded object be main
Raw material produces method of the fly maggot culture medium to cultivate fly maggot.
To solve the above problems, the technical solution adopted by the present invention is：
A kind of method that fly maggot breeding is carried out using fruit discarded object, is comprised the following steps：
(1) by discarded apple, yellow peach and banana by weight proportion 1：1-3：1-2 is mixed after progress in pulverizer
The fruit muddy mixed is crushed, fruit muddy is then placed in progress microwave drying sterilization processing in microwave device, finally kept
Water content in fruit muddy is about 30%；
(2) by the fruit muddy in step (1) and dusty yeast, sodium benzoate by weight 20-40：1-2：0.1-0.2 is put into
10-30min is ground in ball mill, microwave sterilization processing is then carried out again, fly maggot culture medium is obtained；
(3) ovum is connect：Culture medium in step (2) is uniformly layed on culture plate, and fly is delivered according to every Kg culture mediums
Fly blow is inoculated on culture medium by ovum 1-1.2g ratio；
(4) culture plate is suppressed into 24-28 DEG C of temperature, air humidity 50-60%, lucifuge state, draughty culturing room
It is middle to carry out culture until continuing to cultivate after larva appearance 4-5 days, then culture plate is taken out from greenhouse and is placed on from unglazed, until nothing
After larva climbs out of out of fly maggot culture, larva is collected.
Wherein, the laying depth of culture medium is about 2-3cm in the step (3).
The ratio for delivering 1.1g according to every Kg culture mediums in the step (3) is inoculated with fly blow.
The power of microwave is 400-800W in the step (1) and step (2).
And it is noted that the cleaning-sterilizing of surrounding environment works in breeding process, and prevent housefly from fleeing.
In addition, a kind of application of fruit discarded object in cultivation fly maggot is also claimed in the present invention, wherein, described fruit
Discarded object is discarded apple, yellow peach and banana by weight proportion 1：1-3：1-2 is mixed after being crushed in pulverizer
Resulting fruit muddy.
The present invention technique effect be：The present invention uses fruit discarded object for primary raw material to prepare fly maggot culture first
Base, and with the nutritional ingredient of fruit muddy and the suitable next further rich medium of ratio collocation of dusty yeast, a side of the invention
Face realizes the recycling of fruit discarded object, protects environment, has on the other hand also saved cost for fly maggot breeding, realized money
Source reasonable disposition utilization.
Embodiment
Technical scheme is further elaborated with reference to embodiment：
Embodiment 1
A kind of method that fly maggot breeding is carried out using fruit discarded object, is comprised the following steps：
(1) by discarded apple, yellow peach and banana by weight proportion 1：2：1.5 are mixed after carrying out powder in pulverizer
The broken fruit muddy mixed, is then placed in progress microwave drying sterilization processing in microwave device by fruit muddy, finally keeps water
Water content in puree is about 30%；
(2) by the fruit muddy in step (1) and dusty yeast, sodium benzoate by weight 30：1.5：0.15 is put into ball mill
In be ground 20min, then again carry out microwave sterilization processing, obtain fly maggot culture medium；
(3) ovum is connect：Culture medium in step (2) is uniformly layed on culture plate, the laying depth of culture medium is about
2cm, and fly blow is inoculated on culture medium according to every Kg culture mediums dispensing fly blow 1.1g ratio；
(4) culture plate is suppressed to carry out in 25 DEG C of temperature, air humidity 55%, lucifuge state, draughty culturing room
Then culture plate is taken out and is placed on from unglazed, until without larva from fly until larva continues to cultivate 4 days after occurring by culture from greenhouse
After being climbed out of in maggot culture, larva is collected.
Embodiment 2
A kind of method that fly maggot breeding is carried out using fruit discarded object, is comprised the following steps：
(1) by discarded apple, yellow peach and banana by weight proportion 1：1：1 is mixed after being crushed in pulverizer
The fruit muddy mixed, is then placed in progress microwave drying sterilization processing in microwave device by fruit muddy, finally keeps fruit
Water content in mud is about 30%；
(2) by the fruit muddy in step (1) and dusty yeast, sodium benzoate by weight 20：1：0.1 is put into ball mill
Row grinding 15min, then carries out microwave sterilization processing, obtains fly maggot culture medium again；
(3) ovum is connect：Culture medium in step (2) is uniformly layed on culture plate, the laying depth of culture medium is about
2cm, and fly blow is inoculated on culture medium according to every Kg culture mediums dispensing fly blow 1g ratio；
(4) culture plate is suppressed to carry out in 24 DEG C of temperature, air humidity 50%, lucifuge state, draughty culturing room
Then culture plate is taken out and is placed on from unglazed, until without larva from fly until larva continues to cultivate 4 days after occurring by culture from greenhouse
After being climbed out of in maggot culture, larva is collected.
Embodiment 3
A kind of method that fly maggot breeding is carried out using fruit discarded object, is comprised the following steps：
(1) by discarded apple, yellow peach and banana by weight proportion 1：3：2 are mixed after being crushed in pulverizer
The fruit muddy mixed, is then placed in progress microwave drying sterilization processing in microwave device by fruit muddy, finally keeps fruit
Water content in mud is about 30%；
(2) by the fruit muddy in step (1) and dusty yeast, sodium benzoate by weight 40：2：0.2 is put into ball mill
Row grinding 10-30min, then carries out microwave sterilization processing, obtains fly maggot culture medium again；
(3) ovum is connect：Culture medium in step (2) is uniformly layed on culture plate, the laying depth of culture medium is about
3cm, and fly blow is inoculated on culture medium according to every Kg culture mediums dispensing fly blow 1.2g ratio；
(4) culture plate is suppressed to carry out in 28 DEG C of temperature, air humidity 60%, lucifuge state, draughty culturing room
Then culture plate is taken out and is placed on from unglazed, until without larva from fly until larva continues to cultivate 5 days after occurring by culture from greenhouse
After being climbed out of in maggot culture, larva is collected.
Embodiment 4
A kind of method that fly maggot breeding is carried out using fruit discarded object, is comprised the following steps：
(1) by discarded apple, yellow peach and banana by weight proportion 1：2：2 are mixed after being crushed in pulverizer
The fruit muddy mixed, is then placed in progress microwave drying sterilization processing in microwave device by fruit muddy, finally keeps fruit
Water content in mud is about 30%；
(2) by the fruit muddy in step (1) and dusty yeast, sodium benzoate by weight 35：1.5：0.1 is put into ball mill
20min is ground, microwave sterilization processing is then carried out again, fly maggot culture medium is obtained；
(3) ovum is connect：Culture medium in step (2) is uniformly layed on culture plate, the laying depth of culture medium is about
2cm, and fly blow is inoculated on culture medium according to every Kg culture mediums dispensing fly blow 1g ratio；
(4) culture plate is suppressed to carry out in 26 DEG C of temperature, air humidity 55%, lucifuge state, draughty culturing room
Then culture plate is taken out and is placed on from unglazed, until without larva from fly until larva continues to cultivate 5 days after occurring by culture from greenhouse
After being climbed out of in maggot culture, larva is collected.
Embodiment 5
Fly-maggot protein matter is extracted with proteinase hydrolization method to 1-4 of the embodiment of the present invention and the gained fly maggot of following comparative example 1,
Specific steps include：
(1) by fly maggot larva by sterilizing and washing, then clean fly maggot larva is added in certain volume water and carries out glue
Worn-off is broken to obtain fly maggot slurry, wherein the mass volume ratio (g/mL) of clean fly maggot larva and water is 1：24；
(2) slurry of step (1) is warming up to 55 DEG C, and adds 2790 alkali proteases and the regular hour is hydrolyzed,
Until trypsase is added when slurry pH drops to 8.5 or so is hydrolyzed a period of time, afterwards until slurry pH is down to 7.5 or so
When add AS1398 neutral proteinases proceed hydrolysis a period of time, wherein, the addition of the protease is step (1)
The 1.5wt% of slurry, and the enzyme activity of protease is 40,000 units/g；
(3) step (2) is hydrolyzed to the slurry after finishing and carries out destroy the enzyme treatment, then carried out initial filter processing, obtain filtrate, initial filter
Filtrate afterwards continues to be fed into milipore filter and carries out refined filtration processing, and the filtrate after refined filtration processing is standby；In the milipore filter refined filtration processing
The aperture of filter membrane is about 50nm, and transmembrane pressure is 0.1MPa, and crossflow velocity is 4.5m/s；
(4) filtrate for obtaining step (3) refined filtration adds a certain amount of amino acid and glucose, at a temperature of 110 DEG C
The deodorant flavouring for reacting about 60min time to carry out filtrate is handled；The addition of the amino acid and glucose is respectively filter
The 0.5% of liquid quality and 0.4%；
(5) spray drying is carried out after finally the reaction solution of step (4) is concentrated and produces the fly-maggot protein matter.
Comparative example 1
A kind of method that fly maggot breeding is carried out using fruit discarded object, is comprised the following steps：
(1) by discarded apple, yellow peach and banana by weight proportion 1：2：1.5 are mixed after carrying out powder in pulverizer
The broken fruit muddy mixed, is then placed in progress microwave drying sterilization processing in microwave device by fruit muddy, finally keeps water
Water content in puree is about 30%；
(2) by the fruit muddy in step (1) and sodium benzoate by weight 30：0.15 is put into ball mill and is ground
20min, then carries out microwave sterilization processing, obtains fly maggot culture medium again；
(3) ovum is connect：Culture medium in step (2) is uniformly layed on culture plate, the laying depth of culture medium is about
2cm, and fly blow is inoculated on culture medium according to every Kg culture mediums dispensing fly blow 1.1g ratio；
(4) culture plate is suppressed to carry out in 25 DEG C of temperature, air humidity 55%, lucifuge state, draughty culturing room
Then culture plate is taken out and is placed on from unglazed, until without larva from fly until larva continues to cultivate 4 days after occurring by culture from greenhouse
After being climbed out of in maggot culture, larva is collected.
The performance of the fly-maggot protein matter of embodiment 1-4 and comparative example 1 to the method acquisition with embodiment 5 carries out evaluation survey
Fixed, assay method is tested using prior art CN10355798 determination techniques：
1. extract the content of protein：Determined using triumphant formula nitriding.
2. the content of fat：Determined using soxhlet extraction.
3. ash content：Determined according to GB/T5009.4-2003 burning constant weight methods.
4. moisture：Determined according to GB/T5009.4-2003 direct drying methods.
5. bitter taste：Using sensory evaluation method, 10 personnel that participate in evaluation and electing (being non-smoker) are randomly selected, prepare liquid 1- is taken
2m1 spues in mouth after 5 seconds.Prepare liquid is by weight 1 by the albumen obtained by extraction:20 be mixed in water be made.Will be to be measured
The bitter taste of liquid is set as 4 ranks：Without bitter taste, bitter taste is small, bitter taste is general, heavy bitter taste.
6. yield：The protein wt of extraction/fly maggot larva initial weight × 100%.
Table 1：Embodiment 1-4 and comparative example 1 the performance test results：
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Comparative example 1 | |
| Protein content % | 95.1 | 94.3 | 93.6 | 94.1 | 90.5 |
| Fat content % | 2.2 | 2.6 | 2.5 | 2.8 | 3.3 |
| Moisture % | 1.3 | 1.5 | 1.7 | 1.7 | 2.3 |
| Ash content % | 0.14 | 0.16 | 0.18 | 0.17 | 0.19 |
| Bitter taste | It is small | It is small | It is small | It is small | It is small |
| Yield % | 35.5 | 36.2 | 35.9 | 36.2 | 26.8 |
As can be seen from Table 1, the Protein yield that extraction process of the invention is extracted has reached higher level, and excellent
In comparative example.
Finally it should be noted that：The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention,
Although the present invention is described in detail with reference to the foregoing embodiments, for those skilled in the art, it still may be used
To be modified to the technical scheme described in foregoing embodiments, or equivalent substitution is carried out to which part technical characteristic.
Within the spirit and principles of the invention, any modification, equivalent substitution and improvements made etc., should be included in the present invention's
Within protection domain.
Claims (6)
1. a kind of method that fly maggot breeding is carried out using fruit discarded object, it is characterised in that comprise the following steps：
(1) by discarded apple, yellow peach and banana by weight proportion 1：1-3：1-2 is mixed after being crushed in pulverizer
The fruit muddy mixed, is then placed in progress microwave drying sterilization processing in microwave device by fruit muddy, finally keeps fruit
Water content in mud is about 30%；
(2) by the fruit muddy in step (1) and dusty yeast, sodium benzoate by weight 20-40：1-2：0.1-0.2 is put into ball milling
10-30min is ground in machine, microwave sterilization processing is then carried out again, fly maggot culture medium is obtained；
(3) ovum is connect：Culture medium in step (2) is uniformly layed on culture plate, and fly blow 1- is delivered according to every Kg culture mediums
Fly blow is inoculated on culture medium by 1.2g ratio；
(4) culture plate is suppressed to enter in 24-28 DEG C of temperature, air humidity 50-60%, lucifuge state, draughty culturing room
Row culture continues to cultivate 4-5 days after larva occurs, and then culture plate is taken out from greenhouse and is placed on from unglazed, up to without larva
After being climbed out of out of fly maggot culture, larva is collected.
2. artificial cultivating method according to claim 1, it is characterised in that the laying of culture medium is thick in the step (3)
Degree is about 2-3cm.
3. artificial cultivating method according to claim 1, it is characterised in that according to every Kg culture mediums in the step (3)
Deliver 1.1g ratio inoculation fly blow.
4. artificial cultivating method according to claim 1, it is characterised in that microwave in the step (1) and step (2)
Power is 400-800W.
5. artificial cultivating method according to claim 1, it is characterised in that in breeding process it is noted that surrounding environment it is clear
Clean disinfection, and prevent housefly from fleeing.
6. application of a kind of fruit discarded object in cultivation fly maggot, it is characterised in that described fruit discarded object is discarded apple
Really, yellow peach and banana by weight proportion 1：1-3：1-2 is mixed the fruit muddy obtained by being crushed in pulverizer.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012000174A1 (en) * | 2010-06-29 | 2012-01-05 | 周星伟 | Method for breeding fly maggots with medicinal value |
| CN103947613A (en) * | 2014-05-09 | 2014-07-30 | 江苏洁净环境科技有限公司 | Cultivation method for fly maggots |
| CN105580778A (en) * | 2016-02-04 | 2016-05-18 | 常州可赛成功塑胶材料有限公司 | Method for preparing high-activity fly maggot protein on basis of soybean dreg bred fly maggots |
| CN105875511A (en) * | 2015-06-09 | 2016-08-24 | 周辛平 | Fly maggot culture method |
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2017
- 2017-04-13 CN CN201710242502.9A patent/CN106973862A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012000174A1 (en) * | 2010-06-29 | 2012-01-05 | 周星伟 | Method for breeding fly maggots with medicinal value |
| CN103947613A (en) * | 2014-05-09 | 2014-07-30 | 江苏洁净环境科技有限公司 | Cultivation method for fly maggots |
| CN105875511A (en) * | 2015-06-09 | 2016-08-24 | 周辛平 | Fly maggot culture method |
| CN105580778A (en) * | 2016-02-04 | 2016-05-18 | 常州可赛成功塑胶材料有限公司 | Method for preparing high-activity fly maggot protein on basis of soybean dreg bred fly maggots |
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