WO2012000174A1 - Procédé pour élever des asticots de mouche à valeur médicinale - Google Patents

Procédé pour élever des asticots de mouche à valeur médicinale Download PDF

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Publication number
WO2012000174A1
WO2012000174A1 PCT/CN2010/074717 CN2010074717W WO2012000174A1 WO 2012000174 A1 WO2012000174 A1 WO 2012000174A1 CN 2010074717 W CN2010074717 W CN 2010074717W WO 2012000174 A1 WO2012000174 A1 WO 2012000174A1
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WO
WIPO (PCT)
Prior art keywords
parts
fly
maggot
breeding
house
Prior art date
Application number
PCT/CN2010/074717
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English (en)
Chinese (zh)
Inventor
陈建平
Original Assignee
周星伟
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 周星伟 filed Critical 周星伟
Priority to PCT/CN2010/074717 priority Critical patent/WO2012000174A1/fr
Publication of WO2012000174A1 publication Critical patent/WO2012000174A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates

Definitions

  • the invention relates to the technical field of breeding and breeding, and more particularly to a method for breeding maggots with medicinal value. Background technique
  • the breeding ability of flies is in the forefront of insects.
  • the larvae of flies are called fly maggots, which grow very fast.
  • the maggot protein is a high-quality protein.
  • antibacterial active protein, compound amino acid and emu oil can be extracted from maggots. , such as chitin, not only high-quality feed, but also protein powder, high-grade nutritional products, aerospace foods, medicines, etc., so it has a wide range of uses in medicine, health care products, chemicals and other fields.
  • the artificial breeding of flies originated in the late 1970s and early 1980s. Although the fly maggot culture has been proposed for more than 20 years, it has not yet reached the scale. In the rural areas, the cultivation in the countryside cannot be warmed up.
  • the level of science and technology is relevant, and the high-efficiency aseptic cage method just makes up for this.
  • the high-efficiency aseptic cage technology is popular and easy to understand. It is suitable for both small-scale farms and larger farms and aquaculture farms. However, the most direct use of the current breeding of maggots is still to serve as a feed and cannot meet the needs of health foods and medicinal products.
  • the Chinese Patent Office has disclosed a large-scale breeding method and equipment for maggots.
  • the bulletin number is CN1149013C.
  • This document discloses a large-scale breeding method and equipment for maggots.
  • the fly-flying equipment for large-scale breeding of maggots is closed by breeding. Box, feather tray, connecting cloth bag, plastic sheet for feeding, plastic sheet for collecting eggs, sponge for drinking water; larval culture equipment consists of larval incubator and larval culture room; seed mash feed according to designed nutrients, proportion and process , fly bait and commodities ⁇ feeding; breeding, management of fly, adult and commercial cockroaches according to design scale and production process.
  • the fly maggot cultivated by this method is only for providing a feed protein and an edible protein, and cannot meet the demand for medicinal use. Summary of the invention
  • the artificial breeding method of maggots cannot meet the needs of health foods and medicinal materials, and in order to solve the deficiencies in the prior art, the present invention proposes a breeding method with medicinal value of maggots.
  • a method of breeding medicinal value of maggots including adult fly breeding and fly maggot breeding, and the breeding method comprises the following steps:
  • the fly is placed in the fly-flying room.
  • the fly-fly room is a translucent fly house surrounded by a glass fiber reinforced plastic roof and surrounded by a nylon mesh.
  • the fly-fly room has a rope and
  • the egg pots are placed in the egg pots, and the animal protein is used for attracting the eggs to concentrate on spawning.
  • the animal protein is a mixture of fish paste or mash or a mixture of the two in any proportion; the fly feed is prepared in the following parts by weight: 80 to 95 parts of milk powder, 1 to 3 parts of brown sugar, 10 to 15 parts of fruit;
  • the eggs produced by the adult flies are placed in the maggot chamber, and the roof of the maggot chamber is covered with black shade net material, and the maggot chamber has a pond and a collecting pond;
  • the feed is composed of 25 to 100 parts of animal protein, 0 to 30 parts of vegetables, and 3 to 5 parts of anti-cancer factors, wherein the animal protein is a combination of two or more of frog, freshwater fish and animal internal organs.
  • the anticancer factor is any one or a combination of two or more of the yew leaf, the wolfberry venom or the apiary.
  • the adult fly uses Chrysomyia megacephala as a species fly, which has the characteristics of fast reproduction, strong disease resistance and high temperature resistance, so that it does not degenerate to ensure efficacy. Since this species is a special fly species, it is not required. "Proovin", its egg-laying quantity can fully meet the design requirements, and the eggs cultivated by the culture method using the invention grow up as a species fly, and the medicinal value is more obvious.
  • the fly-forming room is 10 to 20 cubic meters, and the feeding amount is 100,000 to 200,000 adult flies, the temperature is controlled within the range of 28 ° C to 35 ° C, and the adult fly house is hung 8 to 20 rope.
  • the fly-flying room hangs the rope for the fly to rest. The number of ropes to be hung depends on the size of the house.
  • the "big-headed gold fly” is characterized by civil, active, not easy to raise, high feeding requirements, and comparison of temperature conditions. high.
  • a feeding pond is placed every 1.5 to 2 square meters in the maggot chamber, and the temperature of the feeding pond is controlled within a range of 30 ° C to 40 ° C.
  • This ventilation condition is good, and the pond has the advantages of acid removal, firmness and gas permeability.
  • the oyster pool is made of glue, wood slag, steel bar and plywood.
  • the glue is made of light burnt magnesium powder mixed with magnesium chloride and water to form a gel, wherein the light burnt magnesium powder and magnesium chloride are used.
  • the weight ratio is 15 ⁇ 25: 75 ⁇ 85.
  • the wood slag is made of odorless wood residue, and the plywood is also made of odorless material.
  • the wood slag, the round steel bar and the plywood are bonded by the light-burning magnesium powder and magnesium chloride which are adjusted into glue.
  • the setting of the pond is conducive to the growth of maggots and facilitates the management of maggots.
  • a light source is provided on the pond.
  • the light source may be a lighting device such as an electric lamp.
  • the invention utilizes the characteristics of the long fly maggots to protect the light from the front, and adopts the light collecting method to turn on the light, and most of the maggots will automatically climb away from the bait to the darker. Close to the pool, unhealthy cockroaches, dead cockroaches and debris and natural and healthy cockroaches are naturally separated, facilitating the collection of maggots.
  • the freshwater fish is salmon, squid, squid or grass carp
  • the animal viscera is porcine lung
  • the vegetable is any one or a combination of two or more of cabbage, tomato or cucumber.
  • the fly maggot feed comprises, by weight: 15 to 80 parts of frog, 10 to 30 parts of freshwater fish, 5 to 8 parts of tomato, 5 to 8 parts of cabbage, 3 to 4 parts of yew leaf, and 0.3 to 0.6 of wolfberry poison Share.
  • the fly maggot feed comprises, in parts by weight: 15 to 80 parts of pig lungs, 10 to 30 parts of freshwater fish, 5 to 8 parts of tomatoes, 5 to 8 parts of cabbage, 3 to 4 parts of yew leaves, and 0.3 to dew. 0.6 parts.
  • the fly maggot feed comprises, in parts by weight: 30 to 50 parts of the frog, 30 to 50 parts of the pig lung, 5 to 20 parts of the cabbage, 2.4 to 4 parts of the yew leaf, and 0.6 to 1 part of the apiary.
  • the fly maggot feed comprises, by weight: 15 to 35 parts of frog, 15 to 35 parts of pig lung, 10 to 30 parts of freshwater fish, 3 to 5 parts of tomato, 3 to 5 parts of cucumber, 3 to 5 parts of cabbage, 2 to 4 parts of yew leaves, 0.2 to 0.3 parts of wolf venom, and 0.6 to 1 part of dew.
  • the above feed can be fed freshly after being processed slightly. After several years of testing by the National New Drug Screening Center, the above-mentioned feeder-fed maggots have a strong inhibition rate on various tumors and bacterial growth, and their anti-tumor biological activities are 80%-97%.
  • the beneficial effects of the present invention are:
  • the medicinal value of the fly maggot cultured according to the present invention has been tested by the National New Drug Screening Center and related departments for several years, and has strong growth for various tumors and bacteria.
  • the inhibition rate, its anti-tumor biological activity is between 80% and 97%. detailed description
  • the invention selects "Golden-headed Golden Fly” as the fly species, "Chrysomyia megacephala”. Because the fly species is a special fly species, it does not need “oval”, and its egg-laying quantity can fully meet the design requirements. At the same time, the egg cultivated by the culture method of the present invention grows up as a species fly, and the medicinal value is more obvious.
  • the animal protein is Fish paste or mash or a mixture of the two in any ratio; adult fly words: milk powder 80 ⁇ 95 grams, brown sugar 1 ⁇ 3 grams, fruit 10 ⁇ 15 grams, and the right amount of water.
  • the eggs produced by the adult flies are placed in the maggot chamber, and the roof of the maggot chamber is covered with black shade net material.
  • the fly maggot house has a pond and a collecting pond, and the fly A potting pond is placed every 1.5 ⁇ 2 square meters in the boudoir, and the temperature of the pond is controlled within the range of 30 °C ⁇ 40 °C.
  • the oyster pool is made of glue, wood slag, steel bar and plywood.
  • the size is 0. 7 m X 0. 5 m X 0. 6 m.
  • the glue is made of light burnt magnesium powder mixed with magnesium chloride and water to form a gel.
  • the weight ratio of light burned magnesium powder to magnesium chloride is 15 ⁇ 25: 75 ⁇ 85.
  • the wood slag is made of odorless wood slag, and the plywood is also made of odorless material.
  • the slag, the round steel bar and the plywood are bonded by the light-burning magnesium powder and magnesium chloride which are adjusted into glue.
  • the pond made by the above method has the advantages of acid removal, firmness and venting.
  • the maggots are placed in the pool, and the fly maggots are placed at the same time.
  • the temperature in the pond is controlled at about 35 degrees.
  • the fly maggot feed consists of 25 to 100 parts of animal protein, 0 to 30 parts of vegetables and 3 to 5 parts of anti-cancer factors, wherein the animal protein is two or more of frog, freshwater fish and animal internal organs.
  • the combination of the anticancer factor is any one or a combination of two or more of the yew leaf, the wolfberry venom or the apiary.
  • the raw materials of the fly maggot feed are shown in Table 1 in parts by weight, and the various raw materials are pulverized and mixed to form fly maggot feed.
  • Table 1 Formulas of fly maggots of Examples 1 to 12 (unit: kilograms) Frog 15 80 35 15 30 50 40 30 100 Pig lung 35 15 50 30 15 80 30 50 100 Salmon 30 Salmon 20 10 Salmon 15 30 10 Grass fish 30 10 10 Tomato 8 5 3 5 5 8 10 10 3 Cucumber 3 5 10 10 3 Cabbage 5 8 5 3 5 20 8 5 10 10 3 Yew
  • the collected maggots are rinsed to remove ineffective debris, then frozen, thawed, sterilized, rinsed, and dried and powdered at a temperature of 40 ° C to 45 ° C.
  • the powder can be eaten directly and can achieve anti-cancer effects without any separation.
  • the fly maggots obtained from the above examples were made into powder according to the above method, and were tested by the National New Drug Screening Center for a strong inhibition rate on various tumors and bacterial growth, and the antitumor biological activity was 80°/. ⁇ 97%, the specific test results are as follows: 1. National New Drug Screening Center
  • Fine plant KB human squamous cell carcinoma
  • the inhibition rate of tumor fine packet growth is /.
  • Fine package forest BEL-7402 human liver cancer Duration: 72 hours
  • HCT-116 human colon cancer Concentration (mg/ml) 1 0. 25 0. 063 0. 016 0. 0039 Evaluation Remarks Sample Number
  • Tetrazolium salt Metal-Thiazol-Tetrozol ium, MTT
  • the inhibition rate of tumor cell growth is 0 /.
  • Tetrazolium salt Metal-Thiazol-Tetrozol ium, MTT
  • the inhibition rate of tumor cell growth is 0 /.
  • Tetrazolium salt Metal Pu Thiazol- Tetrozol ium, MTT
  • Fine sputum strain A- 549 human sputum cancer
  • the inhibition rate of tumor cell growth is 0 /.
  • Determination method micro liquid dilution method, agar dilution method, judged by minimum inhibitory concentration (MIC) value and minimum bactericidal concentration (MBC) value
  • NC00072302 >100 >100 >100
  • NC00072302 >100 >100 >100
  • NC00072304 >100 >100 >100 Note: The sample is not well water-soluble, and the supernatant is tested during the experiment.

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Fodder In General (AREA)

Abstract

L'invention concerne un procédé pour élever des asticots de mouche à valeur médicinale consistant à élever des mouches adultes et leurs asticots. Un logement de mouche adulte est constitué d'un tissu maillé et d'un film mince en plastique. La partie supérieure de ce logement est composée d'une plaque de verre/acier. Le logement de mouche adulte comprend des fils et des coupelles pour recueillir des mouches de viande. La partie supérieure d'un logement d'asticots est constituée d'un tissu maillé noir obscur. Le logement de mouche adulte comprend un bassin d'élevage d'asticots et un bassin de recueil d'asticots. L'alimentation des mouches adultes est composée de lait en poudre (80-95 parts en poids), de cassonade (1-3 parts en poids) et de fruits (10-15 parts en poids). L'alimentation des asticots est composée de protéines animales (25-100 parts en poids), de légumes (0-30 parts en poids) et de facteurs anticancéreux (3-5 parts en poids). Les protéines animales forment au moins deux combinaisons composées de grenouilles, de poissons d'eau douce et des viscères d'animaux. Les éléments des facteurs anticancéreux sont l'un quelconque ou au moins deux combinaisons de feuilles d'if, de stellera chamaejasme, et de nidus vespae. Les asticots élevés au moyen du procédé présentent un effet d'inhibition satisfaisant sur la croissance de tumeurs et bactéries multiples, et leur activité biologique antinéoplasique est comprise entre 80 et 97%.
PCT/CN2010/074717 2010-06-29 2010-06-29 Procédé pour élever des asticots de mouche à valeur médicinale WO2012000174A1 (fr)

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PCT/CN2010/074717 WO2012000174A1 (fr) 2010-06-29 2010-06-29 Procédé pour élever des asticots de mouche à valeur médicinale

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PCT/CN2010/074717 WO2012000174A1 (fr) 2010-06-29 2010-06-29 Procédé pour élever des asticots de mouche à valeur médicinale

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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102630643A (zh) * 2012-04-20 2012-08-15 湖南九鼎科技（集团）有限公司 一套规模化蝇蛆养殖设备及其使用方法
CN102987060A (zh) * 2012-12-21 2013-03-27 张很文 一种蝇蛆粉的生产工艺
CN103004702A (zh) * 2012-12-30 2013-04-03 西宁天谷农牧科技有限公司 一种利用藏鸡鸡粪制备蝇蛆的方法
CN103125450A (zh) * 2013-02-07 2013-06-05 浙江大学 利用豆渣进行的蝇蛆养殖方法
CN103651266A (zh) * 2013-11-29 2014-03-26 灵璧县林汇家禽育种有限责任公司 一种白金虫的饲养方法
CN106035248A (zh) * 2016-08-14 2016-10-26 何丽凌 一种马用生物饲料家蝇蛆虫养殖方法
CN106035249A (zh) * 2016-08-14 2016-10-26 何丽凌 一种鸡用生物饲料家蝇蛆虫养殖方法
CN106070087A (zh) * 2016-08-14 2016-11-09 何丽凌 一种鸭用生物饲料家蝇蛆虫养殖方法
CN106954591A (zh) * 2017-04-13 2017-07-18 广东人为峰健康管理有限公司 一种高蛋白含量果蝇的人工养殖方法
CN106973862A (zh) * 2017-04-13 2017-07-25 广东人为峰健康管理有限公司 一种利用水果废弃物进行蝇蛆养殖的方法
CN107736309A (zh) * 2017-11-16 2018-02-27 罗乌支 一种含高脂肪蝇蛆的养殖方法
CN107821291A (zh) * 2017-12-19 2018-03-23 济宁景泽信息科技有限公司 一种生态养鱼系统

Citations (5)

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Publication number Priority date Publication date Assignee Title
CN1295791A (zh) * 2000-09-22 2001-05-23 王欧 蝇蛆大规模养殖方法及设备
CN1360824A (zh) * 2000-12-26 2002-07-31 南开大学 工厂化养殖洁净蝇蛆的方法
CN1736186A (zh) * 2005-08-04 2006-02-22 周志钦 具有抗肿瘤生物活性蝇蛆的养殖方法
CN101637147A (zh) * 2009-08-18 2010-02-03 扬州鸿信生物制品有限公司 蝇蛆养殖的方法
CN101695340A (zh) * 2009-10-13 2010-04-21 张超 无菌蝇蛆的养殖方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1295791A (zh) * 2000-09-22 2001-05-23 王欧 蝇蛆大规模养殖方法及设备
CN1360824A (zh) * 2000-12-26 2002-07-31 南开大学 工厂化养殖洁净蝇蛆的方法
CN1736186A (zh) * 2005-08-04 2006-02-22 周志钦 具有抗肿瘤生物活性蝇蛆的养殖方法
CN101637147A (zh) * 2009-08-18 2010-02-03 扬州鸿信生物制品有限公司 蝇蛆养殖的方法
CN101695340A (zh) * 2009-10-13 2010-04-21 张超 无菌蝇蛆的养殖方法

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102630643A (zh) * 2012-04-20 2012-08-15 湖南九鼎科技（集团）有限公司 一套规模化蝇蛆养殖设备及其使用方法
CN102987060A (zh) * 2012-12-21 2013-03-27 张很文 一种蝇蛆粉的生产工艺
CN103004702A (zh) * 2012-12-30 2013-04-03 西宁天谷农牧科技有限公司 一种利用藏鸡鸡粪制备蝇蛆的方法
CN103125450A (zh) * 2013-02-07 2013-06-05 浙江大学 利用豆渣进行的蝇蛆养殖方法
CN103651266A (zh) * 2013-11-29 2014-03-26 灵璧县林汇家禽育种有限责任公司 一种白金虫的饲养方法
CN106035248A (zh) * 2016-08-14 2016-10-26 何丽凌 一种马用生物饲料家蝇蛆虫养殖方法
CN106035249A (zh) * 2016-08-14 2016-10-26 何丽凌 一种鸡用生物饲料家蝇蛆虫养殖方法
CN106070087A (zh) * 2016-08-14 2016-11-09 何丽凌 一种鸭用生物饲料家蝇蛆虫养殖方法
CN106954591A (zh) * 2017-04-13 2017-07-18 广东人为峰健康管理有限公司 一种高蛋白含量果蝇的人工养殖方法
CN106973862A (zh) * 2017-04-13 2017-07-25 广东人为峰健康管理有限公司 一种利用水果废弃物进行蝇蛆养殖的方法
CN107736309A (zh) * 2017-11-16 2018-02-27 罗乌支 一种含高脂肪蝇蛆的养殖方法
CN107821291A (zh) * 2017-12-19 2018-03-23 济宁景泽信息科技有限公司 一种生态养鱼系统

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