CN104222007B8 - A kind of artificial a large amount of methods producing parasitic wasp - Google Patents
A kind of artificial a large amount of methods producing parasitic wasp Download PDFInfo
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- CN104222007B8 CN104222007B8 CN201310254241.4A CN201310254241A CN104222007B8 CN 104222007 B8 CN104222007 B8 CN 104222007B8 CN 201310254241 A CN201310254241 A CN 201310254241A CN 104222007 B8 CN104222007 B8 CN 104222007B8
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- 230000003071 parasitic effect Effects 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 title claims abstract description 31
- 238000004519 manufacturing process Methods 0.000 claims abstract description 18
- 238000004806 packaging method and process Methods 0.000 claims abstract description 14
- 238000009395 breeding Methods 0.000 claims abstract description 11
- 230000001488 breeding effect Effects 0.000 claims abstract description 11
- 239000011159 matrix material Substances 0.000 claims abstract description 11
- 241000196324 Embryophyta Species 0.000 claims description 69
- 241001481304 Vespoidea Species 0.000 claims description 54
- 239000000758 substrate Substances 0.000 claims description 21
- 238000011081 inoculation Methods 0.000 claims description 20
- 241001517923 Douglasiidae Species 0.000 claims description 15
- 230000000384 rearing effect Effects 0.000 claims description 13
- 239000010903 husk Substances 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 9
- 238000009423 ventilation Methods 0.000 claims description 9
- 239000004744 fabric Substances 0.000 claims description 8
- 238000012856 packing Methods 0.000 claims description 8
- 241000209140 Triticum Species 0.000 claims description 6
- 235000021307 Triticum Nutrition 0.000 claims description 6
- 238000007873 sieving Methods 0.000 claims description 6
- 229910052902 vermiculite Inorganic materials 0.000 claims description 6
- 235000019354 vermiculite Nutrition 0.000 claims description 6
- 239000010455 vermiculite Substances 0.000 claims description 6
- 241000238631 Hexapoda Species 0.000 claims description 5
- 235000013601 eggs Nutrition 0.000 claims description 4
- 235000007164 Oryza sativa Nutrition 0.000 claims description 3
- 244000052616 bacterial pathogen Species 0.000 claims description 3
- 230000009286 beneficial effect Effects 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 230000007423 decrease Effects 0.000 claims description 3
- 239000004615 ingredient Substances 0.000 claims description 3
- 235000009566 rice Nutrition 0.000 claims description 3
- 230000008014 freezing Effects 0.000 claims description 2
- 238000007710 freezing Methods 0.000 claims description 2
- 239000002917 insecticide Substances 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 1
- 241000594036 Liriomyza Species 0.000 abstract description 5
- 241000594033 Liriomyza bryoniae Species 0.000 abstract 2
- 238000004064 recycling Methods 0.000 abstract 2
- 239000002023 wood Substances 0.000 description 4
- 241000255925 Diptera Species 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- -1 insect-proof net Substances 0.000 description 3
- 241000209094 Oryza Species 0.000 description 2
- 101150073877 egg-1 gene Proteins 0.000 description 2
- 241000594031 Liriomyza sativae Species 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
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Abstract
Description
技术领域technical field
[0001]本发明涉及一种人工大量生产寄生蜂的方法，属于农业害虫生物防治领域。The present invention relates to a kind of method for artificial mass production of parasitic wasps, belonging to the field of agricultural pest biological control.
背景技术Background technique
[0002] 斑潜蝇和潜叶蝇是农业生产中的两类重要害虫，寄生蜂是防治这两类害虫的重要天敌。虽然我国已商品化的寄生蜂有很多，但斑潜蝇寄生蜂和潜叶蝇寄生蜂则至今尚未实现商品化生产。Liriomyza sativae and leafminer are two types of important pests in agricultural production, and parasitic wasps are important natural enemies for controlling these two types of pests. Although there are many commercial parasitoids in our country, the parasitoids of Liriomyza sativa and leafminer have not yet achieved commercial production.
[0003]尽管现有的饲养方法能够实现斑潜蝇寄生蜂和潜叶蝇寄生蜂的饲养，但不仅仍处于小规模饲养阶段，而且相关其大量生产流程还有待于进一步提升完善。[0003] Although the existing raising method can realize the feeding of Liriomyza sativa L. parasitoids and Leafminer parasitoids, it is not only still in the stage of small-scale rearing, but also related to its mass production process and needs to be further improved and perfected.
发明内容SUMMARY OF THE INVENTION
[0004]本发明的目的是提供一种人工大量生产寄生蜂的方法，其特征是先利用寄主植物饲养猎物，所述猎物是指斑潜蝇和潜叶蝇，两种蝇类单独饲养或混合饲养，再利用所饲养的猎物饲养寄生蜂的方法，所述寄生蜂是指斑潜蝇寄生蜂和潜叶蝇寄生蜂，两种寄生蜂单独生产或混合生产，具体关键操作步骤为:The object of the present invention is to provide a kind of method for artificial mass production of parasitic wasp, it is characterized in that first utilize host plant to raise prey, described prey refers to leaf miner and leaf miner, two kinds of flies are raised separately or mixed Raising, the method for rearing parasitoid wasp by the reared prey, described parasitoid wasp refers to Liriomyza sativa parasitoid and leafminer parasitoid, two kinds of parasitic wasps are produced individually or mixed production, and concrete key operation steps are:
[0005] I)猎物的接种:寄主植物展开第一对真叶后的10天内，将寄主植物连同其种植容器一起移入到猎物接种室内，要求猎物接种室具有足够的密封性，能够确保猎物不会飞出饲养室，按每株寄主植物接入大于2头的比例接入猎物成虫种源，每株寄主植物含有的猎物产卵量平均达40粒以上时，将寄主植物及其种植容器一起从猎物接种室转移到猎物饲养室，并且向猎物接种室移入新的寄主植物，寄主植物每0.5天-3天更换一次，猎物成虫种源量减少时及时补充新的种源。其中，所述种植器具是指用于种植寄主植物的盆、盘、容器等，这些器具内填装有种植基质，寄主植物生长在这些种植基质内。1) Inoculation of prey: within 10 days after the host plant unfolds the first pair of true leaves, move the host plant together with its planting container into the prey inoculation room. It will fly out of the breeding room, and access the adult prey source according to the proportion of each host plant being more than 2. When the average number of eggs of prey contained in each host plant is more than 40, put the host plant and its planting container together. Transfer from the prey inoculation room to the prey rearing room, and move new host plants into the prey inoculation room. The host plants are replaced every 0.5 to 3 days. When the amount of prey adult provenance decreases, new provenances are replenished in time. Wherein, the planting utensils refer to pots, trays, containers, etc. used for planting host plants, these utensils are filled with planting substrates, and the host plants grow in these planting substrates.
[0006] 2)猎物的饲养:在猎物饲养室内给予寄主植物适宜的温湿度和水分，猎物发育到第二龄期后将寄主植物连同种植容器转移到寄生蜂接种室。[0006] 2) Feeding of the prey: give the host plant suitable temperature, humidity and moisture in the prey breeding room, and transfer the host plant together with the planting container to the parasitic wasp inoculation room after the prey develops to the second instar.
[0007] 3)寄生蜂接种:按寄生蜂与猎物卵1:5-1:30的比例接入寄生蜂，I天后将寄主植物及其种植容器转移到寄生蜂饲养室。3) parasitic wasp inoculation: access parasitoid wasp by the ratio of parasitoid wasp and prey egg 1:5-1:30, host plant and planting container thereof are transferred to parasitoid wasp rearing room after 1 day.
[0008] 4)寄生蜂的饲养与收集:在饲养室内寄生蜂饲养4-7天后，用剪刀剪下寄主植物，将植株茎、枝和叶一起放入收集装置中，也可单独剪取寄主植物的叶片或枝叶放入收集装置中，然后尽快密封收集装置，再将收集装置放在合适的空间内，固定放好后即可用于寄生蜂成虫的收集，收集操作是每周检查、收集I次以上，优选每天检查、收集I次以上，直至收集管收集不到寄生蜂为止，收集管是长度在Im以内、用于收集寄生蜂的容器，确保收集不到寄生蜂后即可清理收集装置内的植株茎、枝或叶。4) feeding and collection of parasitic wasp: after 4-7 days of rearing indoor parasitic wasp, cut off host plant with scissors, plant stem, branch and leaf are put into collection device together, also can cut host individually The leaf or branch and leaf of plant are put into the collection device, then the collection device is sealed as soon as possible, then the collection device is placed in a suitable space, can be used for the collection of parasitic wasp adults after being fixed, and the collection operation is to check every week, collect 1 It is preferred to check and collect more than 1 time per day, until the collection tube can not collect parasitic wasps, the collection tube is a container within 1 m in length for collecting parasitic wasps, and the collection device can be cleaned after ensuring that no parasitic wasps are collected. Plant stems, branches or leaves inside.
[0009]其中，所述收集装置是指用于收集寄生蜂的器具，收集装置可由两部分组成也可由三部分组成，优选收集装置由三部分组成，若收集装置由两部分组成则包括收集容器和收集管两部分，收集寄生蜂时，收集管直接与收集容器相连，寄生蜂是从收集容器内直接爬到收集管内；若收集装置由三部分组成则包括收集容器、装在收集容器上的收集导管和收集管三部分，收集寄生蜂时，收集管直接与收集导管相连，寄生蜂是从收集容器内先爬到收集导管内再爬到收集管内。Wherein, described collection device refers to the apparatus that is used to collect parasitic wasp, collection device can be made up of two parts also can be made up of three parts, preferably collection device is made up of three parts, if collection device is made up of two parts then comprises collection container When collecting parasitic wasps, the collection tube is directly connected to the collection container, and the parasitic wasps climb directly into the collection tube from the collection container; if the collection device consists of three parts, it includes the collection container, the The collection conduit and the collection tube are three parts. When collecting parasitic wasps, the collection tube is directly connected to the collection conduit. The parasitic wasps first climb into the collection conduit from the collection container and then climb into the collection tube.
[0010]所述收集装置放在合适的空间是指计划用于寄生蜂收集的空间，要求该空间在寄生蜂收集时有光无光均可，但在固定收集装置、检查更换收集管、清理收集装置内的植株茎、枝或叶时需要有光。Described collection device is placed in suitable space and refers to the space planned for the collection of parasitic wasps, and this space is required to have light or no light when parasitoid is collected, but in fixed collection device, inspection and replacement of collection tubes, cleaning Light is required when collecting plant stems, branches or leaves within the unit.
[0011]所述收集容器是指用于装填寄生蜂及植株茎、枝或叶的部分，收集容器具有0.00 Im3-1m3的容积，收集容器的制造材料必须含有纱网、纱布、布、防虫网、纸或其它能透气又能有效阻止寄生蜂逃逸的材料，所述纱网、纱布、布、防虫网、纸或其它能透气又能有效阻止寄生蜂逃逸的材料可以单独使用也可两种或两种以上混合使用，收集容器还必须具有较好的密封性，能够尽量保证寄生蜂除爬到或飞到收集管内以外无法从其它地方飞出或爬出，收集容器还具有容易打开和封住的开口，打开开口即可填装寄生蜂及植株茎、枝或叶，封住开口即可密封收集容器。Described collection container refers to the part that is used to fill parasitic wasp and plant stem, branch or leaf, collection container has 0.001m3-1m volume, the manufacturing material of collection container must contain gauze, gauze, cloth, insect-proof net , paper or other materials that are breathable and can effectively prevent parasitic wasps from escaping, the gauze, gauze, cloth, insect-proof net, paper or other materials that are breathable and can effectively prevent parasitic wasps from escaping can be used alone or in combination. If two or more types are used in combination, the collection container must also have good sealing performance to ensure that the parasitic wasps cannot fly out or crawl out from other places except to climb or fly into the collection tube. The collection container is also easy to open and seal. The opening can be filled with parasitic wasps and plant stems, branches or leaves, and the opening can be sealed to seal the collection container.
[0012]所述收集导管是指装在收集容器上用于寄生蜂飞出或爬出的通道，寄生蜂可以从收集导管装在收集容器上的那一头飞出或爬出收集容器，再从收集导管与收集管连在一起的那一头飞进或爬进收集管，收集容器上的收集导管数为I个或多个，收集导管的长度在Im以内。Described collection conduit refers to the passage that is installed on the collection container for the parasitic wasp to fly out or climb out, and the parasitic wasp can fly out or climb out of the collection container from the end where the collection conduit is installed on the collection container, and then from the collection container. The end of the collecting duct that is connected with the collecting duct flies into or climbs into the collecting duct, the number of collecting ducts on the collecting container is one or more, and the length of the collecting duct is within 1 m.
[0013] 5)寄生蜂的包装方法:利用收集装置收集到寄生蜂后，按包装标准将寄生蜂成虫放入带有蛭石、木肩、麦壳或谷壳的包装瓶内，然后快速盖紧瓶盖，包装瓶瓶身和瓶盖能够完全密封、能够有效阻止寄生蜂逃逸，寄生蜂包装完后即可应用或继续冷藏，所述包装瓶瓶盖带有透气孔，透气孔大小、形状不固定，但透气孔需被纸或纱网、纱布、布封住，优选透气孔被纸封住。其中所述蛭石、木肩、麦壳或谷壳是指寄生蜂的包装介质，各种包装介质可单独使用，也可两种及以上混合使用。5) the packing method of parasitic wasp: after utilizing collecting device to collect parasitic wasp, the parasitoid adult is put into the packaging bottle with vermiculite, wood chip, wheat husk or chaff by packing standard, then quickly cover Tighten the bottle cap, the bottle body and cap of the packaging bottle can be completely sealed, which can effectively prevent parasitic wasps from escaping. After the parasitic wasps are packaged, they can be used or continue to be refrigerated. The packaging bottle cap is provided with ventilation holes, the size and shape of the ventilation holes Not fixed, but the ventilation holes need to be sealed with paper or gauze, gauze, cloth, preferably the ventilation holes are sealed with paper. The vermiculite, wood shoulder, wheat husk or rice husk refers to the packaging medium of parasitic wasps, and various packaging mediums can be used alone or in combination of two or more.
[0014] 6)种植基质的处理及重新利用:先从寄生蜂饲养室内移出使用过的种植基质，放置0.1天以上，种植基质待达到一定干度后直接过筛或用粉碎机打碎后继续使用，其中若过筛则筛网大小为0.lcm-lOcm，筛网能允许种植基质通过又能防止寄主植物根、茎、叶或其它杂物通过，寄主植物的根茎叶可在粉碎机内打碎后继续使用或不再使用，过筛后或打碎后留下的种植基质可直接利用或与新种植基质混合使用或添加其它有利于寄主植物生长的成分，但在利用这些使用过的种植基质前最好经过冷冻、高温灭虫灭菌或暴晒处理，以确保种植基质内没有寄生蜂、猎物或其它虫类、致病菌等。6) processing and reuse of planting matrix: first remove the used planting matrix from parasitic wasp rearing room, place more than 0.1 day, and continue after planting matrix directly sieves after reaching a certain dryness or smashes with pulverizer Use, wherein if sieving then the size of the screen is 0.1cm-10cm, the screen can allow the planting substrate to pass and can prevent the root, stem, leaf or other debris of the host plant from passing through, and the rhizomes and leaves of the host plant can be in the pulverizer. Continue to use or no longer use after crushing, and the planting substrate left after sieving or crushing can be used directly or mixed with new planting substrate or added with other ingredients that are beneficial to the growth of host plants. Before planting the substrate, it is best to undergo freezing, high temperature insecticide sterilization or exposure to the sun to ensure that there are no parasitic wasps, prey or other insects, pathogenic bacteria, etc. in the planting substrate.
[0015]本发明不仅提供了斑潜蝇、潜叶蝇的饲养方法、寄生蜂的饲养方法，而且还提供了收集寄生蜂的方法和包装寄生蜂的方法，以及种植基质的处理和重新利用方法。本发明为潜叶蝇、斑潜蝇寄生蜂的商业化生产首次提供了一种完整的生产工艺，能够为斑潜蝇类和潜叶蝇类的生物防治提供大量的天敌资源。The present invention not only provides the rearing method of leafminer, leafminer, the rearing method of parasitoid, but also provides the method for collecting parasitoid and the method for packing parasitoid, and the processing of planting substrate and reuse method . The invention provides a complete production process for the commercial production of leafminer and leafminer parasitoid for the first time, and can provide a large amount of natural enemy resources for the biological control of leafminer and leafminer.
具体实施方式detailed description
[0016]以下方法仅是对权利要求和发明内容的实施方式举例，不代表对本权利要求和发明内容的限制。[0016] The following methods are only examples of the implementation of the claims and the content of the invention, and do not represent limitations to the claims and the content of the invention.
[0017]实施例1猎物的接种:寄主植物展开第一对真叶后的I天内，将寄主植物连同其种植容器一起移入到猎物接种室内，要求猎物接种室具有足够的密封性，能够确保猎物不会飞出饲养室，按每株寄主植物接入大于6头的比例接入猎物成虫种源，每株寄主植物含有的猎物产卵量平均达80粒以上时，将寄主植物及其种植容器一起从猎物接种室转移到猎物饲养室，并且向猎物接种室移入新的寄主植物，寄主植物每I天更换一次，猎物成虫种源量减少时及时补充新的种源。其中，所述种植器具是指用于种植寄主植物的盆、盘、容器等，这些器具内填装有种植基质，寄主植物生长在这些种植基质内。Example 1 Inoculation of prey: within 1 day after the host plant unfolds the first pair of true leaves, move the host plant together with its planting container into the prey inoculation room, requiring the prey inoculation room to have sufficient airtightness to ensure the prey It will not fly out of the breeding room, and the adult prey source is connected to the ratio of more than 6 heads per host plant. When the average number of eggs laid by the prey contained in each host plant reaches more than 80, the host plants and their planting containers are placed. At the same time, transfer from the prey inoculation room to the prey breeding room, and move new host plants into the prey inoculation room. The host plants are replaced every 1 day, and new provenances are replenished in time when the amount of prey adult provenance decreases. Wherein, the planting utensils refer to pots, trays, containers, etc. used for planting host plants, these utensils are filled with planting substrates, and the host plants grow in these planting substrates.
[0018]实施例2猎物的饲养:在猎物饲养室内给予寄主植物适宜的温湿度和水分，猎物发育到第二龄期后将寄主植物连同种植容器转移到寄生蜂接种室。[0018] Embodiment 2 Prey feeding: give the host plants suitable temperature, humidity and moisture in the prey breeding room, and transfer the host plants together with the planting container to the parasitic wasp inoculation room after the prey develops to the second instar.
[0019]实施例3寄生蜂接种:按寄生蜂与猎物卵1:15的比例接入寄生蜂，I天后将寄主植物及其种植容器转移到寄生蜂饲养室。Embodiment 3 parasitic wasp inoculation: access parasitoid wasp by the ratio of parasitoid wasp and prey egg 1:15, host plant and its planting container are transferred to parasitic wasp rearing room after 1 day.
[0020]实施例4寄生蜂的饲养与收集:在饲养室内寄生蜂饲养5天后，用剪刀剪下寄主植物，将植株茎、枝和叶一起放入收集装置中，也可单独剪取寄主植物的叶片或枝叶放入收集装置中，然后尽快密封收集装置，再将收集装置放在合适的空间内，固定放好后即可用于寄生蜂成虫的收集，收集操作是每周检查、收集I次以上，优选每天检查、收集I次以上，直至收集管收集不到寄生蜂为止，收集管是长度在Im以内、用于收集寄生蜂的容器，确保收集不到寄生蜂后即可清理收集装置内的植株茎、枝或叶。The feeding and collection of embodiment 4 parasitoids: after 5 days of rearing indoor parasitoids, cut off host plant with scissors, plant stem, branch and leaf are put into collection device together, also can cut host plant separately The leaves or branches and leaves are put into the collection device, then the collection device is sealed as soon as possible, then the collection device is placed in a suitable space, and can be used for the collection of parasitic wasp adults after being fixed, and the collection operation is to check every week, collect 1 time Above, it is preferable to check and collect more than 1 time a day, until the collection tube can not collect parasitic wasps, the collection tube is a container with a length of less than 1m, used to collect parasitic wasps, and the collection device can be cleaned after ensuring that no parasitic wasps are collected. stems, branches or leaves of plants.
[0021]其中，所述收集装置是指用于收集寄生蜂的器具，收集装置可由两部分组成也可由三部分组成，优选收集装置由三部分组成，若收集装置由两部分组成则包括收集容器和收集管两部分，收集寄生蜂时，收集管直接与收集容器相连，寄生蜂是从收集容器内直接爬到收集管内；若收集装置由三部分组成则包括收集容器、装在收集容器上的收集导管和收集管三部分，收集寄生蜂时，收集管直接与收集导管相连，寄生蜂是从收集容器内先爬到收集导管内再爬到收集管内。Wherein, described collection device refers to the apparatus that is used to collect parasitic wasp, and collection device can be made up of two parts or can also be made up of three parts, preferably collection device is made up of three parts, if collection device is made up of two parts then comprises collection container When collecting parasitic wasps, the collection tube is directly connected to the collection container, and the parasitic wasps climb directly into the collection tube from the collection container; if the collection device consists of three parts, it includes the collection container, the The collection conduit and the collection tube are three parts. When collecting parasitic wasps, the collection tube is directly connected to the collection conduit. The parasitic wasps first climb into the collection conduit from the collection container and then climb into the collection tube.
[0022]所述收集装置放在合适的空间是指计划用于寄生蜂收集的空间，要求该空间在寄生蜂收集时有光无光均可，但在固定收集装置、检查更换收集管、清理收集装置内的植株茎、枝或叶时需要有光。Described collection device is placed in suitable space and refers to the space planned for the collection of parasitic wasps, and it is required that this space has light or no light when parasitic wasps collect, but when fixing collection device, inspecting and replacing collection tubes, cleaning up. Light is required when collecting plant stems, branches or leaves within the unit.
[0023]所述收集容器是指用于装填寄生蜂及植株茎、枝或叶的部分，收集容器具有Described collection container refers to the part that is used for filling parasitic wasp and plant stem, branch or leaf, and collection container has
0.00 Im3-1m3的容积，收集容器的制造材料必须含有纱网、纱布、布、防虫网、纸或其它能透气又能有效阻止寄生蜂逃逸的材料，所述纱网、纱布、布、防虫网、纸或其它能透气又能有效阻止寄生蜂逃逸的材料可以单独使用也可两种或两种以上混合使用，收集容器还必须具有较好的密封性，能够尽量保证寄生蜂除爬到或飞到收集管内以外无法从其它地方飞出或爬出，收集容器还具有容易打开和封住的开口，打开开口即可填装寄生蜂及植株茎、枝或叶，封住开口即可密封收集容器。The volume of 0.00 Im3-1m3, the manufacturing materials of the collection container must contain gauze, gauze, cloth, insect-proof net, paper or other materials that are breathable and can effectively prevent parasitic wasps from escaping. , paper or other materials that are breathable and can effectively prevent parasitic wasps from escaping can be used alone or in combination of two or more. It cannot fly or climb out of the collection tube from other places. The collection container also has an opening that is easy to open and seal. Open the opening to fill the parasitic wasps and plant stems, branches or leaves, and seal the opening to seal the collection container. .
[0024]所述收集导管是指装在收集容器上用于寄生蜂飞出或爬出的通道，寄生蜂可以从收集导管装在收集容器上的那一头飞出或爬出收集容器，再从收集导管与收集管连在一起的那一头飞进或爬进收集管，收集容器上的收集导管数为I个或多个，收集导管的长度在Im以内。Described collection conduit refers to the passage that is installed on the collection container for the parasitic wasp to fly out or climb out, and the parasitic wasp can fly out or climb out of the collection container from the end where the collection conduit is installed on the collection container, and then from the collection container. The end of the collecting duct that is connected with the collecting duct flies into or climbs into the collecting duct, the number of collecting ducts on the collecting container is one or more, and the length of the collecting duct is within 1 m.
[0025]实施例5寄生蜂的包装方法:利用收集装置收集到寄生蜂后，按包装标准将寄生蜂成虫放入带有蛭石、木肩、麦壳或谷壳的包装瓶内，然后快速盖紧瓶盖，包装瓶瓶身和瓶盖能够完全密封、能够有效阻止寄生蜂逃逸，寄生蜂包装完后即可应用或继续冷藏，所述包装瓶瓶盖带有透气孔，透气孔大小、形状不固定，但透气孔需被纸或纱网、纱布、布封住，优选透气孔被纸封住。其中所述蛭石、木肩、麦壳或谷壳是指寄生蜂的包装介质，各种包装介质可单独使用，也可两种及以上混合使用。The packing method of embodiment 5 parasitic wasp: after utilizing collecting device to collect parasitic wasp, by packing standard, parasitic wasp imago is put into the packaging bottle with vermiculite, wood chip, wheat husk or chaff, then quickly Close the cap tightly, the bottle body and cap of the packaging bottle can be completely sealed, which can effectively prevent parasitic wasps from escaping, and the parasitic wasps can be used or continue to be refrigerated after being packaged. The shape is not fixed, but the ventilation holes need to be sealed with paper or gauze, gauze, cloth, preferably the ventilation holes are sealed with paper. The vermiculite, wood shoulder, wheat husk or rice husk refers to the packaging medium of parasitic wasps, and various packaging mediums can be used alone or in combination of two or more.
[0026]实施例6种植基质的处理及重新利用:先从寄生蜂饲养室内移出使用过的种植基质，放置0.1天以上，种植基质待达到一定干度后直接过筛或用粉碎机打碎后继续使用，其中若过筛则筛网大小为0.lcm-lOcm，筛网能允许种植基质通过又能防止寄主植物根、茎、叶或其它杂物通过，寄主植物的根茎叶可在粉碎机内打碎后继续使用或不再使用，过筛后或打碎后留下的种植基质可直接利用或与新种植基质混合使用或添加其它有利于寄主植物生长的成分，但在利用这些使用过的种植基质前最好经过冷冻、高温灭虫灭菌或暴晒处理，以确保种植基质内没有寄生蜂、猎物或其它虫类、致病菌等。The processing of embodiment 6 planting matrix and reuse: first remove used planting matrix from parasitoid breeding room, place more than 0.1 day, planting matrix directly sieves after reaching certain dryness or after crushing with pulverizer Continue to use, wherein if sieving then the size of the screen is 0.1cm-10cm, the screen can allow the planting substrate to pass and can prevent the root, stem, leaf or other debris of the host plant from passing through, and the rhizomes and leaves of the host plant can be used in the pulverizer. Continue to use or no longer use after internal crushing, and the planting substrate left after sieving or crushing can be used directly or mixed with new planting substrate or added with other ingredients that are beneficial to the growth of host plants, but after using these used It is best to freeze, sterilize at high temperature or expose to the sun before the planting substrate to ensure that there are no parasitic wasps, prey or other insects, pathogenic bacteria, etc. in the planting substrate.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101904320A (en) * | 2010-07-27 | 2010-12-08 | 中山大学 | Method for breeding a large number of microplitis bicoloratus |
| CN102144619A (en) * | 2011-04-13 | 2011-08-10 | 云南省热带作物科学研究所 | Artificial propagation method of diversinervus elegans silvestri |
| CN102578052A (en) * | 2012-03-16 | 2012-07-18 | 宁波市林特科技推广中心 | A bayberry orchard Drosophila elanogaster artificial breeding method, and method for breeding parasitic wasp with the same |
| CN102630647A (en) * | 2012-05-03 | 2012-08-15 | 中国农业科学院植物保护研究所 | Method for propagating Diglyphus isaea |
| CN102771446A (en) * | 2012-08-07 | 2012-11-14 | 中国农业科学院植物保护研究所 | Propagation method of Diglyphus isaea by use of Chromatomyia horticola |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101904320A (en) * | 2010-07-27 | 2010-12-08 | 中山大学 | Method for breeding a large number of microplitis bicoloratus |
| CN102144619A (en) * | 2011-04-13 | 2011-08-10 | 云南省热带作物科学研究所 | Artificial propagation method of diversinervus elegans silvestri |
| CN102578052A (en) * | 2012-03-16 | 2012-07-18 | 宁波市林特科技推广中心 | A bayberry orchard Drosophila elanogaster artificial breeding method, and method for breeding parasitic wasp with the same |
| CN102630647A (en) * | 2012-05-03 | 2012-08-15 | 中国农业科学院植物保护研究所 | Method for propagating Diglyphus isaea |
| CN102771446A (en) * | 2012-08-07 | 2012-11-14 | 中国农业科学院植物保护研究所 | Propagation method of Diglyphus isaea by use of Chromatomyia horticola |
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