CN106561575A - Artificial feeding method for diaphorina citri - Google Patents
Artificial feeding method for diaphorina citri Download PDFInfo
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- CN106561575A CN106561575A CN201610909428.7A CN201610909428A CN106561575A CN 106561575 A CN106561575 A CN 106561575A CN 201610909428 A CN201610909428 A CN 201610909428A CN 106561575 A CN106561575 A CN 106561575A
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- 235000020971 citrus fruits Nutrition 0.000 claims abstract description 14
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- 239000003337 fertilizer Substances 0.000 claims description 51
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- 230000000384 rearing effect Effects 0.000 claims description 18
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- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 claims description 15
- 241000819999 Nymphes Species 0.000 claims description 13
- 241000233866 Fungi Species 0.000 claims description 10
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 10
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 10
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses an artificial feeding method for diaphorina citri. The method includes the following steps that feed plant cultivation and management are conducted, wherein feed plants include murraya paniculata and citrus; woodlice are fed; a woodlouse feeding cage is cleaned; and the feed plants are repaired. According to the method, a large number of woodlouse imagoes and nymphs not carrying Candidatus liberobacter asiaticus can be bred, and a large number of indoor woodlouse insect sources not carrying germs can be provided for areas where few woodlice or no woodlouse exists so as to meet needs of relevant tests.
Description
Technical field
The invention belongs to field of pest control, specifically, is related to a kind of rearing method of diaphorina citri.
Background technology
Diaphorina citri Diaphorina citri (Kuwayama) is a kind of oranges and tangerines tender tip phase important insect, is often resulted in tender
Tip atrophy, deformity, cause dark mildew, affect the photosynthesis of oranges and tangerines, and are to propagate Citrus Huanglongbing pathogen Candidatus
(the Chinese Plants pathology meeting in Deng Mingxue, Peng Youliang, 2006 year of the unique natural medium insects of liberobacter asiaticu
Collection of thesis), Citrus Huanglongbing pathogen is quarantine disease, does not find both at home and abroad effect a radical cure this sick effective ways at present, once oranges and tangerines
Having infected yellow twig can only excavate.Single wood louse adult can spiral broadcast yellow twig, pass sick rate and be up to 70%-80%, and lifelong band
Disease, can closely spread with the wind, and 1-3 ages nymph will not pass disease, but 4-5 ages nymph can pass disease, and ovum is without poison (Bai Huaju, dragon
The dream tinkling of pieces of jade, Citrus Huanglongbing pathogen progress in 2009), in yellow twig epidemic-stricken area, a situation arises decides the generation of yellow twig for wood louse
Rate, very big potentiality is caused to mandarin tree and is threatened.
At present, correlative study has been carried out to local diaphorina citri in the oranges and tangerines producing region that yellow twig seriously occurs, and
The incidence of disease is low and the oranges and tangerines producing region do not fallen ill is not studied wood louse, with global warming, the distributed areas of wood louse by
Gradually move northward.In view of wood louse propagates yellow twig to cause greatly to cause harm to oranges and tangerines, temporarily it is not distributed in wood louse but very likely sends out in the future
Raw region, needs to carry out correlative study to the diaphorina citri under the conditions of local climate in advance, accomplishes to be preventive from possible trouble, therefore, mandarin orange
The raising of tangerine wood louse is most important to wood louse correlative study.
The content of the invention
In view of this, the present invention is directed to above-mentioned problem, there is provided a kind of rearing method of diaphorina citri.
In order to solve above-mentioned technical problem, the invention discloses a kind of rearing method of diaphorina citri, including it is following
Step：
Step 1, raising plant culture and management：Raising plant includes kamuning and oranges and tangerines；
Step 2, wood louse are raised；
Step 3, cleaning wood louse rearging cage；
Plant is raised in step 4, reparation.
Further, the cultivation of the kamuning in step 1 is specific as follows with management：
Step a, kamuning cultivation：Commercial kamuning seed, seed is sowed in the thick vermiculite of the deep humidities of 10cm, and is placed on
In 30-35 DEG C of incubator, 14h illumination：After 10h dark culturings 2 weeks, kamuning can germinate, and open after kamuning seedling grows to 5cm
Begin to transplant, according to the size for transplanting basin, 1-3 strain kamuning seedling is planted in every basin, potting soil is thick vermiculite and irrigation soils 3:1-
1:3 uniform mixing, can not apply fertilizer after transplanting in 2-3 month, and the later stage uses as needed organic flower fertilizer, by a small amount of multiple principle
Applied fertilizer, based on liquid fertilizer, supplemented by solid fertilizer；Just can be used to raise wood louse after the kamuning transplanted grows 1 year；
Step b, kamuning management：With fatty acid potassium salt insecticidal soap and mineral oil 6:4-9:1 mixed atomizing Murraya jasminorage leaf
Piece, and ensure that plant is sufficiently wetted, but should not be sprayed when sunlight strong direct projection, after desinsection process
2d, for raising wood louse；Kamuning is pruned as needed, to promote it to produce new branches and leaves, for wood louse spawning, kamuning repairing
After cutting 2 weeks, i.e., for raising wood louse, prune principle and be to try to control the height of sapling, to be advisable less than 1.0m, make many points of tree
Branch.
Further, the cultivation of the oranges and tangerines in step 1 is specific as follows with management：
Step i, citriculture：The tip and many and eugonic Citrus Cultivars are easily taken out in commercial bitter orange or Fructus Aurantii stock, grafting,
Bud to be grafted cuts stock more than folding grafting bud after sprouting, promote the grafting tip to grow, the tip grow to 5-10cm it is long when cut off previously folding
Disconnected stock, and graft union film is removed, can either be used for raising wood louse after seedling growth 1.5-2 to be grafted；Grafting bud is sprouted
Afterwards, need to be applied fertilizer to stock, by a small amount of multiple principle, based on liquid fertilizer, supplemented by solid fertilizer；
Step ii, oranges and tangerines management：With fatty acid potassium salt insecticidal soap and mineral oil 6:4-9:1 mixed atomizing Citrus leaf,
And ensure that plant is sufficiently wetted, but should not be sprayed when sunlight strong direct projection, otherwise the tender tip easily to newly growing
Position produces poisoning, the 2d after desinsection process, can be used to raise wood louse.Oranges and tangerines are pruned as needed, to promote it to produce
New branches and leaves, for wood louse spawning, oranges and tangerines prune 2-3 it is all after, can be enough to raise wood louse, prune principle and be to try to control and set
The height of seedling, to be advisable less than 1.0m, makes tree multi-branched.
Further, the wood louse of the oranges and tangerines in step 2 is raised specific as follows：
Step 2.1, preparation wood louse rearging cage
Wood louse rearging cage is shaped as cuboid, a height of 1.5mx0.8mx1.2m of length and width, by timber, bamboo, steel these
Hard material constitutes main frame, is coupled together framework with simple iron wire, and size is can place 3 years raw kamuning or oranges and tangerines sapling
It is suitable, using transparent gauze by light penetration, both sides, the back side, front use uvioresistant fine-structure mesh, front to have one at the top of cage
Individual hinged door or slide fastener, for observing, move and operate and raise plant and wood louse；
Step 2.2, wood louse rearing conditions：It is required that temperature is 24-30 DEG C, relative humidity 50-60%, illumination 16h, 8h is black
Secretly；With 4 95 watts of agricultural sodium lamp light fillings in greenhouse, light compensating lamp will be hung over away from the 0.5-1m of rearging cage top, stronger
Illumination will stimulate wood louse adult to produce more ovum；
Step 2.3, wood louse are raised：Wood louse is liked laying eggs in the tender tip of 0.2-1.5cm, pump this tender tip without worm
Evil kamuning or oranges and tangerines Potted orchard are moved in clean wood louse rearging cage with pallet, and 5-10 strains children is placed in each wood louse rearging cage
Seedling, then discharges 500-2000 pollards lice or 100-200 pollards lice/strain in each cage；The more cage the inside wood of young sprout
The burst size of lice is more；After wood louse Adult worms producting eggs 3-4d, the kamuning or oranges and tangerines sapling shaken in rearging cage drives wood louse away
Adult, is then transferred to the kamuning laid eggs or oranges and tangerines sapling new wood louse rearging cage and raises, or test or continuation are used
In breeding wood louse nymph of future generation；Periodically to plant watering fertilising is raised liquid fertilizer is mainly applied in wood louse feeding process, be pressed
A small amount of multiple principle is applied fertilizer.
Further, the cleaning wood louse rearging cage in step 3 is specific as follows：1 generation wood louse is raised, all of wood need to have been removed
Lice, and wood louse rearging cage is cleaned in time；The net cage of wood louse rearging cage is wiped clean with wet sponge, if necessary using gentle soap
Water, therefore, to assure that remove all soapy residues, then sprays again 70% isopropanol and kills any insect, mould or fungi, then puts
After putting 2-3d, that is, can be used in raising wood louse.
Further, the reparation raising plant in step 4 is specific as follows：After having raised 1 generation diaphorina citri, need to repair in time
Raise plant.First with fatty acid potassium salt insecticidal soap and mineral oil 6 in rearging cage:4-9:1 mixed atomizing raises plant leaf blade,
And ensure that plant is sufficiently wetted, spray and remove rearging cage and place 2d plant is raised after mist, then erase raising with wet sponge
The corpse of wood louse nymph, slough, secretion and the mould caused by secretion or fungi, have cleared up after these and have repaiied again on plant
The growing point for raising plant is cut, damaged tissues are removed, new growing point is induced, the reparation that plant is sent in greenhouse will be finally raised
Region, repairing growth is after one month, then prunes raising plant, promotes it to sprout young sprout, to raise wood louse or lay eggs for wood louse
With.
Compared with prior art, the present invention can be obtained including following technique effect：
1) wood louse is raised by indoor culture kamuning or oranges and tangerines seedling, can provide simultaneously a large amount of different larval instar wood louse nymphs,
Different days phase adult is used for the correlative study of wood louse.
2) present invention can cultivate a large amount of wood louses without yellow twig germ into, nymph, be available for wood louse to send out less or do not occur
Area provides indoor wood louse worm sources of not carrying disease germs in a large number, for needed for correlation test.
3) can make up that wood louse sends out less or non-generating region is to the research of wood louse blank shape, for prevention and control yellow twig in advance technology is provided
Support.
Certainly, the arbitrary product for implementing the present invention it is not absolutely required to while reaching all the above technique effect.
Description of the drawings
Accompanying drawing described herein is used for providing a further understanding of the present invention, constitutes the part of the present invention, this
Bright schematic description and description does not constitute inappropriate limitation of the present invention for explaining the present invention.In the accompanying drawings：
Fig. 1 is that the present invention repairs the impact for raising plant to raising wood louse；
Fig. 2 is that the present invention does not repair the impact for raising plant to raising wood louse.
Specific embodiment
Embodiments of the present invention are described in detail below in conjunction with embodiment, thereby to the present invention how application technology hand
Section come solve technical problem and reach technology effect realize that process can fully understand and implement according to this.
Embodiment 1
The present invention provides a kind of rearing method of diaphorina citri, comprises the following steps：
1 raises plant culture with management
1.1 kamuning are cultivated and management
1.1.1 kamuning cultivation
Commercial kamuning seed, seed is sowed in the thick vermiculite of the deep humidities of 10cm, and is placed in 32-34 DEG C of incubator,
14h illumination：After 10h dark culturings 2 weeks, kamuning can germinate, and start to transplant after kamuning seedling grows to 5cm, according to transplanting
The size of basin, plants 1-3 strain kamuning seedling in every basin, potting soil is thick vermiculite and irrigation soils 1:1 uniform mixing, 2- after transplanting
Can not apply fertilizer in 3 months, the later stage can use as needed organic flower fertilizer, be applied fertilizer by a small amount of multiple principle, with liquid fertilizer
Based on, supplemented by solid fertilizer.Just can be used to raise wood louse after the kamuning transplanted grows 1 year.Such as it is badly in need of kamuning and raises wood
Lice, also can directly buy potted plant kamuning.
1.1.2 kamuning management
The Major Pests of kamuning have red spider, aphid and aleyrodid.With fatty acid potassium salt insecticidal soap and mineral oil 8:2 mix
Spraying kamuning blade is closed, and ensures that plant is sufficiently wetted, but should not be sprayed when sunlight strong direct projection, otherwise
Tender tip position easily to newly growing produces poisoning, the 2d after desinsection process, can be used to raise wood louse.Nine are pruned as needed
In it is fragrant, to promote it to produce new branches and leaves, for wood louse spawning, kamuning just can be used to raise wood louse, repaiies after pruning 2 weeks
Cut principle to be to try to control the height of sapling, to be advisable less than 1.0m, make tree multi-branched.
1.2 citricultures and management
1.2.1 citriculture
The tip and many and eugonic Citrus Cultivars are easily taken out in commercial bitter orange or Fructus Aurantii stock, grafting, such as late-maturing navel orange, lemon
Lemon, shaddock etc., bud to be grafted cuts stock more than folding grafting bud after sprouting, promote the grafting tip to grow, the tip grow to 5-10cm it is long when
The stock for previously having fractureed is cut off, and removes graft union film, just can be used to raise wood louse after seedling growth 1.5-2 to be grafted.
After grafting bud is sprouted, need to be applied fertilizer to stock, by a small amount of multiple principle, based on liquid fertilizer, supplemented by solid fertilizer.Such as it is badly in need of mandarin orange
Tangerine seedling raises wood louse, also can directly buy the oranges and tangerines seedling for having cultivated.Oranges and tangerines seedling is typically only capable to for raising without yellow twig disease
The diaphorina citri of bacterium, otherwise can make mandarin tree infection yellow twig and slowly failing can not take out young sprout and dead because wood louse carries disease germs.
1.2.2 oranges and tangerines management
The Major Pests of oranges and tangerines have the larva of red spider, aleyrodid, coccid, prodenia litura.Use fatty acid potassium salt Insecticidal fertilizer
Soap and mineral oil 8:2 mixed atomizing Citrus leafs, and ensure that plant is sufficiently wetted, but should not be when sunlight strong direct projection
Sprayed, otherwise the tender tip position easily to newly growing produces poisoning, the 2d after desinsection process can be used to raise wood louse.
Oranges and tangerines are pruned as needed, and to promote it to produce new branches and leaves, for wood louse spawning, oranges and tangerines just can be used to after pruning 2-3 is all
Wood louse is raised, principle is pruned and is to try to control the height of sapling, to be advisable less than 1.0m, make tree multi-branched.
Step 2, wood louse are raised
2.1 prepare wood louse rearging cage
Wood louse rearging cage can constitute main frame by hard materials such as timber, bamboo, steel, be needed to make not according to oneself
The simple iron wire of same specification, nylon yarn cylinder mould or tent cylinder mould etc., size with can place 3 years raw kamuning or oranges and tangerines sapling be it is suitable,
The insect cage of a height of 1.5mx0.8mx1.2m of length and width can generally be made.The top of cage can allow light to be worn using transparent gauze
Thoroughly, there are a hinged door or slide fastener (simple cylinder mould) in both sides, the back side, front uvioresistant fine-structure mesh, front, for observing, moving
Plant and wood louse are raised in dynamic and operation.
2.2 wood louse rearing conditions
Need can control in warm, wet, light greenhouse, can be heated up with heater, air-conditioning temperature-reducing such as can not be controlled wet, can use
Humidifier control is wet.It is required that temperature is 27 ± 3 DEG C, relative humidity 50-60%, illumination 16h, 8h is dark.With 4 95 watts in greenhouse
Special agricultural sodium lamp light filling, light compensating lamp will be hung over away from the 0.5-1m of rearging cage top, and stronger illumination will stimulate wood louse adult
Produce more ovum.
2.3 wood louses are raised
Wood louse is liked laying eggs in the tender tip of 0.2-1.5cm, pump this tender tip without insect pest kamuning or oranges and tangerines basin
Plant seedling to move in clean rearging cage with pallet, 5-10 strain seedling is placed in each insect cage, then discharge in each cage
500-2000 pollards lice or 100-200 pollards lice/strain.The burst size of the more cage the inside wood louses of young sprout is more.Treat wood louse
After Adult worms producting eggs 3-4d, the kamuning or oranges and tangerines sapling shaken in rearging cage drives wood louse adult away, nine for then being laid eggs
In fragrant or oranges and tangerines sapling be transferred to new insect cage and raise, or test or continue on for breeding wood louse nymph of future generation.In wood louse
Periodically to plant watering fertilising is raised liquid fertilizer is mainly applied in feeding process, be applied fertilizer by a small amount of multiple principle.
3. rearging cage is cleared up
1 generation wood louse is raised, all of wood louse need to have been removed, and cleaned rearging cage in time.The net cage Mare Humorum of rearging cage
Silk floss is wiped clean, it may be necessary to using gentle suds, but, it is ensured that removes all soapy residues, then again sprinkler body product is dense
Spend and kill any insect, mould or fungi for 70% isopropanol, then place after 2-3d, can be used to raise wood louse.
4. repair and raise plant
After having raised 1 generation diaphorina citri, raising plant need to be in time repaired.First fatty acid potassium salt Insecticidal fertilizer is used in rearging cage
Soap and mineral oil 8:2 mixed atomizings raise plant leaf blade, and ensure that plant is sufficiently wetted, and have sprayed and removed plant is raised after mist
Rearging cage simultaneously places 2d, then corpse, slough, secretion (honeydew and the wax for raising wood louse nymph on plant is erased with wet sponge
Matter) and the mould that caused by secretion or fungi, clear up after these and pruned the growing point for raising plant again, remove impaired group
Knit, induce new growing point, will finally raise the restoring area that plant is sent in greenhouse, repairing growth is after one month, then prunes
Plant is raised, promotes it to sprout young sprout, to raise wood louse or lay eggs for wood louse.
Embodiment 2
A kind of rearing method of diaphorina citri, comprises the following steps：
Step 1, raising plant culture and management：Raising plant includes kamuning and oranges and tangerines；
Wherein, the cultivation of kamuning is specific as follows with management：
Step a, kamuning cultivation：Commercial kamuning seed, seed is sowed in the thick vermiculite of the deep humidities of 10cm, and is placed on
In 30-35 DEG C of incubator, 14h illumination：After 10h dark culturings 2 weeks, kamuning can germinate, and open after kamuning seedling grows to 5cm
Begin to transplant, according to the size for transplanting basin, 1-3 strain kamuning seedling is planted in every basin, potting soil is thick vermiculite and irrigation soils 3:1 is equal
Even mixing, can not apply fertilizer after transplanting in 2-3 month, and the later stage uses as needed organic flower fertilizer, carry out by a small amount of multiple principle
Fertilising, based on liquid fertilizer, supplemented by solid fertilizer；Just can be used to raise wood louse after the kamuning transplanted grows 1 year；
Step b, kamuning management：With fatty acid potassium salt insecticidal soap and mineral oil 6:4 mixed atomizing kamuning blades, and
Ensure that plant is sufficiently wetted, but should not be sprayed when sunlight strong direct projection that the 2d after desinsection process is used for
Raise wood louse；Kamuning is pruned as needed, and to promote it to produce new branches and leaves, for wood louse spawning, kamuning is being pruned 2 weeks
Afterwards, i.e., prune principle and be to try to control the height of sapling for raising wood louse, to be advisable less than 1.0m, make tree multi-branched.
The cultivation of oranges and tangerines is specific as follows with management：
Step i, citriculture：The tip and many and eugonic Citrus Cultivars are easily taken out in commercial bitter orange or Fructus Aurantii stock, grafting,
Bud to be grafted cuts stock more than folding grafting bud after sprouting, promote the grafting tip to grow, the tip grow to 5-10cm it is long when cut off previously folding
Disconnected stock, and graft union film is removed, can either be used for raising wood louse after seedling growth 1.5-2 to be grafted；Grafting bud is sprouted
Afterwards, need to be applied fertilizer to stock, by a small amount of multiple principle, based on liquid fertilizer, supplemented by solid fertilizer；
Step ii, oranges and tangerines management：With fatty acid potassium salt insecticidal soap and mineral oil 6:4 mixed atomizing Citrus leafs, and protect
Plant is sufficiently wetted card, but should not be sprayed when sunlight strong direct projection, otherwise the tender tip position easily to newly growing
Poisoning is produced, the 2d after desinsection process can be used to raise wood louse.Oranges and tangerines are pruned as needed, to promote its generation new
Branches and leaves, for wood louse spawning, oranges and tangerines prune 2-3 it is all after, can be enough raising wood louse, prune principle and be to try to control sapling
Highly, to be advisable less than 1.0m, tree multi-branched is made.
Step 2, wood louse are raised：
Step 2.1, preparation wood louse rearging cage
Wood louse rearging cage is shaped as cuboid, a height of 1.5mx0.8mx1.2m of length and width, by timber, bamboo, steel these
Hard material constitutes main frame, is coupled together framework with simple iron wire, and size is can place 3 years raw kamuning or oranges and tangerines sapling
It is suitable, using transparent gauze by light penetration, both sides, the back side, front use uvioresistant fine-structure mesh, front to have one at the top of cage
Individual hinged door or slide fastener, for observing, move and operate and raise plant and wood louse；
Step 2.2, wood louse rearing conditions：It is required that temperature is 24-30 DEG C, relative humidity 50-60%, illumination 16h, 8h is black
Secretly；With 4 95 watts of agricultural sodium lamp light fillings in greenhouse, light compensating lamp will be hung over away from the 0.5-1m of rearging cage top, stronger
Illumination will stimulate wood louse adult to produce more ovum；
Step 2.3, wood louse are raised：Wood louse is liked laying eggs in the tender tip of 0.2-1.5cm, pump this tender tip without worm
Evil kamuning or oranges and tangerines Potted orchard are moved in clean wood louse rearging cage with pallet, and 5-10 strains children is placed in each wood louse rearging cage
Seedling, then discharges 500-2000 pollards lice or 100-200 pollards lice/strain in each cage；The more cage the inside wood of young sprout
The burst size of lice is more；After wood louse Adult worms producting eggs 3-4d, the kamuning or oranges and tangerines sapling shaken in rearging cage drives wood louse away
Adult, is then transferred to the kamuning laid eggs or oranges and tangerines sapling new wood louse rearging cage and raises, or test or continuation are used
In breeding wood louse nymph of future generation；Periodically to plant watering fertilising is raised liquid fertilizer is mainly applied in wood louse feeding process, be pressed
A small amount of multiple principle is applied fertilizer.
Step 3, cleaning wood louse rearging cage：
1 generation wood louse is raised, all of wood louse need to have been removed, and cleaned wood louse rearging cage in time；The net cage of wood louse rearging cage
Wiped clean with wet sponge, if necessary using gentle suds, therefore, to assure that remove all soapy residues, then spray again
70% isopropanol kills any insect, mould or fungi, then places after 2-3d, that is, can be used in raising wood louse.
Plant is raised in step 4, reparation：
After having raised 1 generation diaphorina citri, raising plant need to be in time repaired.First fatty acid potassium salt Insecticidal fertilizer is used in rearging cage
Soap and mineral oil 6:4 mixed atomizings raise plant leaf blade, and ensure that plant is sufficiently wetted, and have sprayed and removed plant is raised after mist
Rearging cage simultaneously places 2d, then is erased with wet sponge and raise the corpse of wood louse nymph on plant, slough, secretion and by secretion
The mould for causing or fungi, have cleared up after these and have pruned the growing point for raising plant again, remove damaged tissues, induce new growth
Point, will finally raise the restoring area that plant is sent in greenhouse, and repairing growth is after one month, then prunes raising plant, promotes it
Young sprout is sprouted, to raise wood louse or lay eggs for wood louse.
Embodiment 3
A kind of rearing method of diaphorina citri, comprises the following steps：
Step 1, raising plant culture and management：Raising plant includes kamuning and oranges and tangerines；
Wherein, the cultivation of kamuning is specific as follows with management：
Step a, kamuning cultivation：Commercial kamuning seed, seed is sowed in the thick vermiculite of the deep humidities of 10cm, and is placed on
In 30-35 DEG C of incubator, 14h illumination：After 10h dark culturings 2 weeks, kamuning can germinate, and open after kamuning seedling grows to 5cm
Begin to transplant, according to the size for transplanting basin, 1-3 strain kamuning seedling is planted in every basin, potting soil is thick vermiculite and irrigation soils 1:3 is equal
Even mixing, can not apply fertilizer after transplanting in 2-3 month, and the later stage uses as needed organic flower fertilizer, carry out by a small amount of multiple principle
Fertilising, based on liquid fertilizer, supplemented by solid fertilizer；Just can be used to raise wood louse after the kamuning transplanted grows 1 year；
Step b, kamuning management：With fatty acid potassium salt insecticidal soap and mineral oil 9:1 mixed atomizing kamuning blade, and
Ensure that plant is sufficiently wetted, but should not be sprayed when sunlight strong direct projection that the 2d after desinsection process is used for
Raise wood louse；Kamuning is pruned as needed, and to promote it to produce new branches and leaves, for wood louse spawning, kamuning is being pruned 2 weeks
Afterwards, i.e., prune principle and be to try to control the height of sapling for raising wood louse, to be advisable less than 1.0m, make tree multi-branched.
The cultivation of oranges and tangerines is specific as follows with management：
Step i, citriculture：The tip and many and eugonic Citrus Cultivars are easily taken out in commercial bitter orange or Fructus Aurantii stock, grafting,
Bud to be grafted cuts stock more than folding grafting bud after sprouting, promote the grafting tip to grow, the tip grow to 5-10cm it is long when cut off previously folding
Disconnected stock, and graft union film is removed, can either be used for raising wood louse after seedling growth 1.5-2 to be grafted；Grafting bud is sprouted
Afterwards, need to be applied fertilizer to stock, by a small amount of multiple principle, based on liquid fertilizer, supplemented by solid fertilizer；
Step ii, oranges and tangerines management：With fatty acid potassium salt insecticidal soap and mineral oil 9:1 mixed atomizing Citrus leaf, and protect
Plant is sufficiently wetted card, but should not be sprayed when sunlight strong direct projection, otherwise the tender tip position easily to newly growing
Poisoning is produced, the 2d after desinsection process can be used to raise wood louse.Oranges and tangerines are pruned as needed, to promote its generation new
Branches and leaves, for wood louse spawning, oranges and tangerines prune 2-3 it is all after, can be enough raising wood louse, prune principle and be to try to control sapling
Highly, to be advisable less than 1.0m, tree multi-branched is made.
Step 2, wood louse are raised：
Step 2.1, preparation wood louse rearging cage
Wood louse rearging cage is shaped as cuboid, a height of 1.5mx0.8mx1.2m of length and width, by timber, bamboo, steel these
Hard material constitutes main frame, is coupled together framework with simple iron wire, and size is can place 3 years raw kamuning or oranges and tangerines sapling
It is suitable, using transparent gauze by light penetration, both sides, the back side, front use uvioresistant fine-structure mesh, front to have one at the top of cage
Individual hinged door or slide fastener, for observing, move and operate and raise plant and wood louse；
Step 2.2, wood louse rearing conditions：It is required that temperature is 24-30 DEG C, relative humidity 50-60%, illumination 16h, 8h is black
Secretly；With 4 95 watts of agricultural sodium lamp light fillings in greenhouse, light compensating lamp will be hung over away from the 0.5-1m of rearging cage top, stronger
Illumination will stimulate wood louse adult to produce more ovum；
Step 2.3, wood louse are raised：Wood louse is liked laying eggs in the tender tip of 0.2-1.5cm, pump this tender tip without worm
Evil kamuning or oranges and tangerines Potted orchard are moved in clean wood louse rearging cage with pallet, and 5-10 strains children is placed in each wood louse rearging cage
Seedling, then discharges 500-2000 pollards lice or 100-200 pollards lice/strain in each cage；The more cage the inside wood of young sprout
The burst size of lice is more；After wood louse Adult worms producting eggs 3-4d, the kamuning or oranges and tangerines sapling shaken in rearging cage drives wood louse away
Adult, is then transferred to the kamuning laid eggs or oranges and tangerines sapling new wood louse rearging cage and raises, or test or continuation are used
In breeding wood louse nymph of future generation；Periodically to plant watering fertilising is raised liquid fertilizer is mainly applied in wood louse feeding process, be pressed
A small amount of multiple principle is applied fertilizer.
Step 3, cleaning wood louse rearging cage；
1 generation wood louse is raised, all of wood louse need to have been removed, and cleaned wood louse rearging cage in time；The net cage of wood louse rearging cage
Wiped clean with wet sponge, if necessary using gentle suds, therefore, to assure that remove all soapy residues, then spray again
70% isopropanol kills any insect, mould or fungi, then places after 2-3d, that is, can be used in raising wood louse.
Plant is raised in step 4, reparation：
After having raised 1 generation diaphorina citri, raising plant need to be in time repaired.First fatty acid potassium salt Insecticidal fertilizer is used in rearging cage
Soap and mineral oil 9:1 mixed atomizing raises plant leaf blade, and ensures that plant is sufficiently wetted, and has sprayed and removed plant is raised after mist
Rearging cage simultaneously places 2d, then is erased with wet sponge and raise the corpse of wood louse nymph on plant, slough, secretion and by secretion
The mould for causing or fungi, have cleared up after these and have pruned the growing point for raising plant again, remove damaged tissues, induce new growth
Point, will finally raise the restoring area that plant is sent in greenhouse, and repairing growth is after one month, then prunes raising plant, promotes it
Young sprout is sprouted, to raise wood louse or lay eggs for wood louse.
Wherein, repair raising plant has large effect to raising wood louse, and Jing often repairs raising plant and is conducive to breeding wood
Lice, as shown in figure 1, do not repair raising plant, plant take out young sprout seldom and can slowly natural death, plant do not take out young sprout, wood louse
Adult will not lay eggs, and not laying eggs cannot breed wood louse, as shown in Fig. 2 so must be repaired.
Described above illustrates and describes some preferred embodiments of invention, but as previously mentioned, it should be understood that invention is not
Form disclosed herein is confined to, the exclusion to other embodiment is not to be taken as, and can be used for various other combinations, modification
And environment, and can be carried out by the technology or knowledge of above-mentioned teaching or association area in invention contemplated scope described herein
Change.And change that those skilled in the art are carried out and change be without departing from the spirit and scope of invention, then all should be in the appended power of invention
In the protection domain that profit is required.
Claims (6)
1. a kind of rearing method of diaphorina citri, it is characterised in that comprise the following steps：
Step 1, raising plant culture and management：Raising plant includes kamuning and oranges and tangerines；
Step 2, wood louse are raised；
Step 3, cleaning wood louse rearging cage；
Plant is raised in step 4, reparation.
2. the rearing method of diaphorina citri according to claim 1, it is characterised in that the kamuning in step 1
Cultivation is specific as follows with management：
Step a, kamuning cultivation：Commercial kamuning seed, seed is sowed in the thick vermiculite of the deep humidities of 10cm, and is placed on 30-
In 35 DEG C of incubators, 14h illumination：After 10h dark culturings 2 weeks, kamuning can germinate, and start after kamuning seedling grows to 5cm
Transplant, according to the size for transplanting basin, 1-3 strain kamuning seedling is planted in every basin, potting soil is thick vermiculite and irrigation soils 3:1-1:3
Uniform mixing, can not apply fertilizer after transplanting in 2-3 month, and the later stage uses as needed organic flower fertilizer, enter by a small amount of multiple principle
Row fertilising, based on liquid fertilizer, supplemented by solid fertilizer；Just can be used to raise wood louse after the kamuning transplanted grows 1 year；
Step b, kamuning management：With fatty acid potassium salt insecticidal soap and mineral oil 6:4-9:1 mixed atomizing kamuning blade, and
Ensure that plant is sufficiently wetted, but should not be sprayed when sunlight strong direct projection that the 2d after desinsection process is used for
Raise wood louse；Kamuning is pruned as needed, and to promote it to produce new branches and leaves, for wood louse spawning, kamuning is being pruned 2 weeks
Afterwards, i.e., prune principle and be to try to control the height of sapling for raising wood louse, to be advisable less than 1.0m, make tree multi-branched.
3. the rearing method of diaphorina citri according to claim 1, it is characterised in that the cultivation of the oranges and tangerines in step 1
Training is specific as follows with management：
Step i, citriculture：The tip and many and eugonic Citrus Cultivars are easily taken out in commercial bitter orange or Fructus Aurantii stock, grafting, wait to transfer
Budder sprout after cut folding grafting bud more than stock, promote the grafting tip grow, the tip grow to 5-10cm it is long when cut off what is previously fractureed
Stock, and graft union film is removed, can either be used for raising wood louse after seedling growth 1.5-2 to be grafted；After grafting bud is sprouted, need
To apply fertilizer to stock, by a small amount of multiple principle, based on liquid fertilizer, supplemented by solid fertilizer；
Step ii, oranges and tangerines management：With fatty acid potassium salt insecticidal soap and mineral oil 6:4-9:1 mixed atomizing Citrus leaf, and protect
Plant is sufficiently wetted card, but should not be sprayed when sunlight strong direct projection, otherwise the tender tip position easily to newly growing
Poisoning is produced, the 2d after desinsection process can be used to raise wood louse.Oranges and tangerines are pruned as needed, to promote its generation new
Branches and leaves, for wood louse spawning, oranges and tangerines prune 2-3 it is all after, can be enough raising wood louse, prune principle and be to try to control sapling
Highly, to be advisable less than 1.0m, tree multi-branched is made.
4. the rearing method of diaphorina citri according to claim 1, it is characterised in that the wood of the oranges and tangerines in step 2
Lice is raised specific as follows：
Step 2.1, preparation wood louse rearging cage
Wood louse rearging cage is shaped as cuboid, a height of 1.5mx0.8mx1.2m of length and width, by timber, bamboo, steel these hard
Material constitutes main frame, is coupled together framework with simple iron wire, and size is can place 3 years raw kamuning or oranges and tangerines sapling
Suitable, using transparent gauze by light penetration, both sides, the back side, front uvioresistant fine-structure mesh, front has one at the top of cage
Hinged door or slide fastener, for observing, move and operate and raise plant and wood louse；
Step 2.2, wood louse rearing conditions：It is required that temperature is 24-30 DEG C, relative humidity 50-60%, illumination 16h, 8h is dark；
With 4 95 watts of agricultural sodium lamp light fillings in greenhouse, light compensating lamp will be hung over away from the 0.5-1m of rearging cage top, and stronger illumination will
Wood louse adult can be stimulated to produce more ovum；
Step 2.3, wood louse are raised：Wood louse is liked laying eggs in the tender tip of 0.2-1.5cm, pump this tender tip without insect pest nine
In fragrant or oranges and tangerines Potted orchard move in clean wood louse rearging cage with pallet, 5-10 strain seedling is placed in each wood louse rearging cage,
Then 500-2000 pollards lice or 100-200 pollards lice/strain are discharged in each cage；The more cage the inside wood louses of young sprout
Burst size is more；After wood louse Adult worms producting eggs 3-4d, the kamuning or oranges and tangerines sapling shaken in rearging cage drives wood louse adult away,
Then the kamuning laid eggs or oranges and tangerines sapling are transferred to into new wood louse rearging cage to raise, or test or continue on for breeding
Wood louse nymph of future generation；Periodically to plant watering fertilising is raised liquid fertilizer is mainly applied, by a small amount of many in wood louse feeding process
Secondary principle is applied fertilizer.
5. the rearing method of diaphorina citri according to claim 1, it is characterised in that the cleaning wood louse in step 3
Rearging cage is specific as follows：1 generation wood louse is raised, all of wood louse need to have been removed, and cleaned wood louse rearging cage in time；Wood louse rearging cage
Net cage wiped clean with wet sponge, if necessary using gentle suds, therefore, to assure that remove all soapy residues, then
70% isopropanol is sprayed again and kills any insect, mould or fungi, then placed after 2-3d, that is, can be used in raising wood louse.
6. the rearing method of diaphorina citri according to claim 1, it is characterised in that the reparation in step 4 is raised
Plant is specific as follows：After having raised 1 generation diaphorina citri, raising plant need to be in time repaired.First fatty acid potassium salt is used in rearging cage
Insecticidal soap and mineral oil 6:4-9:1 mixed atomizing raises plant leaf blade, and ensures that plant is sufficiently wetted, and has sprayed after mist feeding
Foster plant removes rearging cage and places 2d, then corpse, slough, the secretion for raising wood louse nymph on plant is erased with wet sponge
And the mould that caused by secretion or fungi, clear up after these and pruned the growing point for raising plant again, damaged tissues are removed, lure
The growing point for sending out new, will finally raise the restoring area that plant is sent in greenhouse, and repairing growth is after one month, then prunes raising plant
Thing, promotes it to sprout young sprout, to raise wood louse or lay eggs for wood louse.
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Cited By (5)
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| CN107821340A (en) * | 2017-12-13 | 2018-03-23 | 肇庆学院 | The breeding apparatus and wood louse method for breeding of a kind of diaphorina citri |
| CN113519466A (en) * | 2021-08-05 | 2021-10-22 | 广州威佰昆生物科技有限公司 | Method for feeding diaphorina citri by tissue culture murraya paniculata |
| CN113693028A (en) * | 2021-08-05 | 2021-11-26 | 广州威佰昆生物科技有限公司 | Method for treating diaphorina citri by microinjection |
| CN113693030A (en) * | 2021-08-26 | 2021-11-26 | 华南农业大学 | Method for artificially transfecting exogenous insect symbiotic bacteria Wolbachia into diaphorina citri |
| CN114717335A (en) * | 2022-05-10 | 2022-07-08 | 赣南师范大学 | Method for obtaining CLas infected diaphorina citri polypide body |
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| CN107821340B (en) * | 2017-12-13 | 2023-05-09 | 肇庆学院 | Device and method for feeding diaphorina citri |
| CN113519466A (en) * | 2021-08-05 | 2021-10-22 | 广州威佰昆生物科技有限公司 | Method for feeding diaphorina citri by tissue culture murraya paniculata |
| CN113693028A (en) * | 2021-08-05 | 2021-11-26 | 广州威佰昆生物科技有限公司 | Method for treating diaphorina citri by microinjection |
| CN113693030A (en) * | 2021-08-26 | 2021-11-26 | 华南农业大学 | Method for artificially transfecting exogenous insect symbiotic bacteria Wolbachia into diaphorina citri |
| CN114717335A (en) * | 2022-05-10 | 2022-07-08 | 赣南师范大学 | Method for obtaining CLas infected diaphorina citri polypide body |
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