CN107821340A - The breeding apparatus and wood louse method for breeding of a kind of diaphorina citri - Google Patents
The breeding apparatus and wood louse method for breeding of a kind of diaphorina citri Download PDFInfo
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- CN107821340A CN107821340A CN201711323423.7A CN201711323423A CN107821340A CN 107821340 A CN107821340 A CN 107821340A CN 201711323423 A CN201711323423 A CN 201711323423A CN 107821340 A CN107821340 A CN 107821340A
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- 238000009395 breeding Methods 0.000 title claims abstract description 39
- 230000001488 breeding effect Effects 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 23
- 235000013305 food Nutrition 0.000 claims abstract description 104
- 241000238631 Hexapoda Species 0.000 claims abstract description 52
- 230000002265 prevention Effects 0.000 claims abstract description 51
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- 238000012258 culturing Methods 0.000 claims abstract description 4
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- 238000004382 potting Methods 0.000 claims description 20
- 241000207199 Citrus Species 0.000 claims description 12
- 235000020971 citrus fruits Nutrition 0.000 claims description 12
- 239000003895 organic fertilizer Substances 0.000 claims description 12
- 102000002322 Egg Proteins Human genes 0.000 claims description 10
- 108010000912 Egg Proteins Proteins 0.000 claims description 10
- 210000004681 ovum Anatomy 0.000 claims description 10
- 229910017464 nitrogen compound Inorganic materials 0.000 claims description 9
- 239000004576 sand Substances 0.000 claims description 6
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- 230000000903 blocking effect Effects 0.000 claims description 3
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Cultivation Of Plants (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
The invention discloses a kind of breeding apparatus of diaphorina citri and wood louse method for breeding.The breeding apparatus includes the insect prevention string bag, food source plant Culture basin and flower-pot cover, and the described insect prevention string bag is placed on the upper end open of food source plant Culture basin, is collectively forming wood louse culturing room with food source plant Culture basin, described food source plant Culture basin is placed in flower-pot cover.The breeding apparatus belongs to multi-functional aggregate device, available for the collection of wood louse sample, transport, isolated rearing and observation, and the cultivation management of nursery stock, can meet the needs of wood louse adult, nymph, alternation of generations raising, and long-term, continuous experimental observation demand, simplicity, the weather conditions controllability and anti-tampering of wood louse raising and observation are realized, it is simple to operate, it is easy to use.
Description
Technical field
The invention belongs to biology and agriculture field, the breeding apparatus of more particularly to a kind of diaphorina citri and wood louse raising side
Method.
Background technology
Citrus is the first in the world major class fruit.And one of important fruits industry in China, the cultivated area of China's citrus
Occupied first place in the world with yield.Citrus Huanglongbing pathogen (Citrus Huanglongbing, HLB) is a kind of systemic disease, and
Quarantine disease.It is one of disease for endangering citrus production most serious, there is no effective radical cure method.
And diaphorina citri (Diaphorina citri (kuwayama)) is to propagate unique natural medium of citrus yellow shoot disease
Insect, there is high characteristic of disease, migrating property and quick fertility, the biography disease rate of single wood louse of passing to be up to 70%~80%, 4~5 ages
Nymph can also pass disease, and the wood louse for carrying Citrus Huanglongbing pathogen pathogeny can be all the life with poison.Therefore research diaphorina citri, formed corresponding
Pest control technology, it is vital.
In addition to being investigated in orchard wood louse, it is also necessary to raise substantial amounts of wood louse and be used for desk research.
Method currently used for raising wood louse mainly has：
1. solarium's method for breeding：Insect-proof net chamber is established, ground plants kamuning or citrus, and wood louse of being gone and offered one's services in solarium is raised, and grinds
Solarium's development correlative study operation can be entered by isolating door by studying carefully personnel.Its advantage is that space is big, can plant big tree, simulates fruit
Garden natural environment；Great amount of samples can be studied.Its shortcoming is the need for certain practice ground condition, easily by bad burst
The influence of climate change.
2. rearging cage method for breeding：It is the main method of current indoor culture.It is rectangular with potted plant kamuning or citrus seedling, outer cover
Body rearging cage, rearging cage main frame are hard material, cage top and side the spun yarn nets such as timber, steel, and part Experiment room uses
The convenient operation of a wicket (or not opening the door) is opened in glass material, side.Its advantage is that environmental condition is controllable, and floor space is small, can be used
In small sample quantifier elimination, its shortcoming is that main frame can cause to observe dead angle, is unfavorable for observing, and water drenching, fertilising needs lift feeding
Support cage or open wicket, it is inconvenient for operation, and the activity of wood louse can be disturbed, or even have observation sample and " escape " event hair
It is raw, the defects of certain be present.
3. Tube propagation method：The method utilizes the branch or blade of the small containers such as test tube, built-in kamuning or citrus, protects
Temperature, wood louse adult or nymph are launched, short-term experimental design observation item is completed, the advantage is that test tube can be positioned over artificial gas
Wait in case, form stationary temperature, illumination, reach the homogeneity of control environmental condition；Small volume；It is micro- entity can be placed directly within
Operational observations under mirror.Its shortcoming is can not to carry out large sample size, long-term continuous experimental implementation.
Therefore, a kind of breeding apparatus of diaphorina citri is designed, simplicity, weather conditions to realizing wood louse raising and observation
Controllability and anti-tampering significant.
The content of the invention
The shortcomings that primary and foremost purpose of the present invention is to overcome prior art and deficiency, there is provided a kind of raising dress of diaphorina citri
Put, realize simplicity, the weather conditions controllability and anti-tampering of wood louse raising and observation.
Another object of the present invention is to provide a kind of method for breeding of diaphorina citri.
The purpose of the present invention is achieved through the following technical solutions：A kind of breeding apparatus of diaphorina citri, including the insect prevention string bag,
Food source plant Culture basin and flower-pot cover, the described insect prevention string bag are placed on food source plant Culture basin, described food source plant Culture basin
It is placed in flower-pot cover.
The described insect prevention string bag is placed on the upper end open of food source plant Culture basin, and wood is collectively forming with food source plant Culture basin
Lice culturing room.
The described insect prevention string bag is 40~60 mesh insect prevention mesh bags；The insect prevention mesh bag of preferably 40~60 mesh, antiultraviolet.
The bore of the described insect prevention string bag is more than the bore of food source plant Culture basin.
The opening of the described insect prevention string bag is additionally provided with tying.
Described tying is nylon rope tying；Preferably high-strength nylon rope tying.
Described food source plant Culture basin is cylindrical food source plant Culture basin；Preferably cylindrical plastics food source plant training
Support basin.
Described food source plant Culture basin also includes thread-locking ring, and described thread-locking ring is threadedly attached to food
The upper end open of source plant Culture basin, while the insect prevention string bag is fixed on food source plant Culture basin.
The side and bottom of described food source plant Culture basin are additionally provided with aperture.
The diameter of described aperture is preferably 0.5cm.
Described aperture is uniformly distributed.
The bottom of described food source plant Culture basin also includes the foot pad of projection, makes between food source plant Culture basin and flower-pot cover
Form certain space.
The number of described foot pad is two or more；Preferably three.
The basin body that described flower-pot cover is cylindrical type, diameter is bigger more than 2cm than the diameter of food source plant Culture basin, or be opening
Diameter is more than base diameter, and the basin body that base diameter is bigger more than 2cm than the diameter of food source plant Culture basin.
The height of described flower-pot cover is half of food source plant Culture basin height or more.
The side of described flower-pot cover also includes draining aperture and the plug for blocking draining aperture.
The positional distance flower-pot cover substructure height of described draining aperture is less than or equal to 0.1cm.
The number of described draining aperture is preferably 1.
The big I of described draining aperture is set according to being actually needed, preferably diameter 1cm draining aperture.
A kind of method for breeding of diaphorina citri, comprises the following steps：
(1) the food source plant of diaphorina citri is planted in food source plant Culture basin, is then placed in food source plant Culture basin
In flower-pot cover, liquid manure or water are noted in flower-pot cover, acted on by self-priming, complete rich water quality management, obtain food source plant；
(2) diaphorina citri adult, nymph or ovum are gathered, is placed in the insect prevention string bag, kind in step (1) is placed on and is implanted with
On the food source plant Culture basin of food source plant, beam lives tying, and tightens thread-locking ring, into the artificial feeding stage.
Food source plant described in step (1) is preferably kamuning or citrus.
The potting soil in food source plant Culture basin described in step (1) is peat soil, loess and thin river sand；Preferably mud
Charcoal soil, loess and thin river sand by volume 2:2:The potting soil that 1 proportioning obtains.
Liquid manure described in step (1) is liquid organic fertilizer and water-soluble high-nitrogen compound fertilizer；It is preferably first that liquid is organic
Fertile and water-soluble high-nitrogen compound fertilizer in mass ratio 1:1 proportioning mixing, then it is diluted to the liquid manure that concentration is 5% (w/w).
The frequency of administration of liquid manure described in step (1) is preferably to apply liquid manure 1 time in every 4 weeks.
The method for breeding of described diaphorina citri, in addition to the food source plant described in step (1) is pinched
The step of processing.
Rich water quality management described in step (1) also includes arranging unnecessary liquid manure or water by the draining aperture of flower-pot cover side
The step of going out, the liquid manure of discharge are available for secondary use.
The method for breeding of described diaphorina citri, in addition to carry out day ordinary water by food source plant is obtained in step (1)
The step of part management：During potting soil arid, water is injected in flower-pot cover, is acted on by the self-priming of potting soil, completes moisture management, it is more
Remaining water is discharged by the draining aperture of flower-pot cover side.
Food source plant described in step (1) obtains preferably by the method described in following any one：
(a) seed of food source plant is soaked, after vernalization by warm water, transplanting to the food source plant equipped with potting soil is cultivated
In basin, then food source plant Culture basin is placed in flower-pot cover, and liquid manure is imposed by flower-pot cover, is acted on by the self-priming of potting soil,
Water and fertilizer management is completed, when potting soil arid, water is injected in flower-pot cover, is acted on by self-priming, completes moisture management, when plant grows
To 10cm or so, topping treatment is carried out to branch, obtains food source plant；
(b) sapling of food source plant is transplanted into the food source plant Culture basin equipped with potting soil, then by food source plant
Culture basin is put in flower-pot cover, places more than 1 week slow seedling, and carries out topping treatment to each branch of food source plant, and is applied by flower-pot cover
With liquid manure, liquid manure is noted in flower-pot cover, is acted on by the self-priming of potting soil, completes water and fertilizer management, using inspiring young sprout after liquid manure,
After young sprout grows out, food source plant is obtained.
Described warm water immersion is preferably 55 DEG C of warm water immersion 45mim.
Described vernalization is preferably the vernalization in growth cabinet；It it is 25 DEG C~27 DEG C preferably in temperature, humidity is
Vernalization in 80% growth cabinet.
Collection diaphorina citri adult, nymph or ovum described in step (2) are realized preferably by following steps：Adopt in orchard
Collect wood louse adult, or the small stems for having diaphorina citri ovum or nymph on fruit tree is cut, be positioned in the insect prevention string bag, and beam lives to prick
Mouthful.
Diaphorina citri adult in the method for breeding of described diaphorina citri, in addition to migration step (3) after artificial feeding
The step of：The branch of food source plant is patted, diaphorina citri adult is migrated onto the insect prevention string bag, then turns on food source plant
Thread-locking ring on Culture basin, unties the tying of the insect prevention string bag, then lifts the insect prevention string bag, and after departing from plant scope, beam is lived anti-
Worm string bag mouth simultaneously tightens tying, and covers the new insect prevention string bag again on food source plant Culture basin, tightens tying and tightens spiral shell
Line retaining ring, ovum or nymph on branch enter the breeding cycle of next round.
The present invention is had the following advantages relative to prior art and effect：
1st, breeding apparatus belongs to multi-functional aggregate device, covers wood louse sample collection, transport, isolated rearing, observation, nursery stock cultivation
Training management etc., and it is simple to operate, it is easy to use.
2nd, breeding apparatus can be placed in growth cabinet, and indoor and outdoor, suitable varying environment research needs.
3rd, wood louse adult, nymph, the needs of alternation of generations raising can be met, and long-term, continuous experimental observation needs
Will.
4th, the moisture content of nursery stock and nutrient management, " exempt to beat from food source plant Culture basin external management, formation wood louse raising
Disturb " pattern.
5th, 360 ° of wood louse observation without dead angle, allows correlation test operation to become more easily and accurate.
Brief description of the drawings
Fig. 1 is diaphorina citri breeding apparatus structure chart of the present invention.
Fig. 2 is the structure chart of the insect prevention string bag of the present invention.
Fig. 3 is the structure chart of food source plant Culture basin of the present invention.
Fig. 4 is the structure chart of the screw thread mouth of food source plant Culture basin of the present invention.
Fig. 5 is the structure chart of the thread-locking ring of diaphorina citri breeding apparatus of the present invention.
Fig. 6 is the basin bottom part structure chart of food source plant Culture basin of the present invention.
In figure：1 is the insect prevention string bag, and 2 be food source plant Culture basin, and 3 be flower-pot cover, and 4 be thread-locking ring, and 5 be tying, and 6 are
Aperture, 7 be screw thread, and 8 be foot pad.
Embodiment
With reference to embodiment, the present invention is described in further detail, but the implementation of the present invention is not limited to this.
Embodiment 1
A kind of breeding apparatus of diaphorina citri, including the insect prevention string bag 1, food source plant Culture basin 2 and flower-pot cover 3；
The described insect prevention string bag 1 is placed on food source plant Culture basin 2；Described food source plant Culture basin 2 is placed in flower-pot cover 3
In；
The described insect prevention string bag 1 is 40~60 mesh, the insect prevention mesh bag of antiultraviolet, and its bore is more than food source plant Culture basin
2 bore, its length, width can be according to the big little makings of food source plant；
The opening of the described insect prevention string bag 1 is provided with tying 5；Described tying 5 is high-strength nylon rope tying；
The described insect prevention string bag 1 is placed at the upper end open of food source plant Culture basin 2, common with food source plant Culture basin 2
Form wood louse culturing room；The insect prevention string bag 1 can be used for the wood louse of shipment collection, and as isolation wood louse on breeding apparatus and extraneous ring
The guard in border：For shipping the wood louse of collection in use, wood louse adult, nymph or ovum are positioned in the insect prevention string bag 1, beam lives to prick
Mouth 5；As Isolation net cover in use, according to the three-dimensional distribution of culture plant, prop up to form certain space naturally, meet
The activity needs of wood louse；
Described food source plant Culture basin 2 is that the vessel are that material is for cultivating the plant vessel such as kamuning, citrus
The cylindrical basin body of plastics；
Described food source plant Culture basin 2 also includes thread-locking ring 4, and thread-locking ring 4 is connected to food source by screw thread 7
The upper end open of plant Culture basin 2；Thread-locking ring 4 is fixed on food source plant Culture basin 2 by screw socket rotation, does not influence to plant
Strain normal growth, while the insect prevention string bag can be fixed on food source plant Culture basin 2, and sack is closed, prevent wood louse from escaping；
The side and bottom of described food source plant Culture basin 2 are provided with aperture 6；Described aperture 6 can be multiple uniform
A diameter of 0.5cm of arrangement aperture, is mainly used in breathing freely, seeps water, and the moisture content of food source plant and nutrient management；
The foot pad 8 that the bottom of described food source plant Culture basin 2 is conformed with, make food source plant Culture basin 2 and flower-pot cover 3 it
Between form certain space；
Described foot pad 8 is three equally distributed foots pad, reaches and excessive moisture control in vessel and ventilated
Purpose；
Described flower-pot cover 3 is the common bowl container for containing object, can be cylindrical type basin body, its diameter is than food source plant
Big more than the 2cm of diameter of Culture basin 2；Can also be that opening diameter is more than base diameter, and base diameter is cultivated than food source plant
Big more than the 2cm of diameter of basin 2 basin body；The height of described flower-pot cover 3 is more than half of the height of food source plant Culture basin 2；Set
When water is placed in basin, acted on by self-priming, for the irrigation of plant, the liquid rich water that dilution is placed in flower-pot cover is used for supporting for plant
Part supply.
The side of described flower-pot cover 3 be additionally provided with draining aperture (the positional distance flower-pot cover substructure height of draining aperture be less than or
Equal to 0.1cm) and for blocking the plug of draining aperture, it is convenient irrigate after unnecessary moisture content and rich water discharged from aperture；
For the big I of described draining aperture according to setting is actually needed, its diameter is preferably 1cm；
The breeding apparatus not regulatory specifications size of the present invention, the dress of different size can be made according to actual kind plant size
Put, being adapted to plantation seed to sprout, seedling is raw by 3 years to be set.
Embodiment 2
A kind of method for breeding of diaphorina citri, specific implementation method：
(1) kamuning seed purchased in market, 55 DEG C of warm water soak 45mim, and inside and outside kind of skin for peelling off kamuning seed (is not hindered
Embryo), it is put in and is lined with the culture dish of moistening filter paper, the vernalization in growth cabinet (25 DEG C~27 DEG C of temperature, humidity 80%), 2
Sprouted in it, radicle, plumule grow to 0.5~2cm in one week.Into food source plant Culture basin 2, potting soil is peat soil for transplanting：
Loess：Thin river sand=2:2:1 (volume ratio), planting depth 1cm, apply liquid manure 1 time within hereafter every 4 weeks, liquid manure has for commercial liquid
The fertile and water-soluble high-nitrogen compound fertilizer of machine (FUBON good fortune nation water soluble organic fertilizer：N-P-K content >=10%, the content of organic matter >=
60%；The refined Ran high-N compound fertilizers of YARA：N:P:K=21:6:13, total nutrient content 40%), by 1:1 (w/w) ratio mixes,
And 5% (w/w) concentration is diluted to, and the liquid manure of this dilution is noted in flower-pot cover 3, acted on by self-priming, completes water and fertilizer management, it is more
Remaining liquid manure is discharged by the draining aperture of the side of flower-pot cover 3, is available for secondary use.During potting soil arid, it can be injected in flower-pot cover 3
Water, acted on by the self-priming of potting soil, complete moisture management, unnecessary water is discharged by the draining aperture of the side of flower-pot cover 3.In nine
Perfume is grown to 10cm, carries out topping treatment to branch, inspires side shoot and young sprout, repeat, until the plentiful tree crown of Plantlet formation,
Suitably raise wood louse.
(2) orchard collection wood louse adult, and be positioned in the insect prevention string bag 1, beam lives tying 5 (high-strength nylon rope tying),
Laboratory is taken back, unties nylon rope tying 5, is placed on to have planted and is implanted with the food source plant Culture basin 2 of kamuning, beam lives nylon rope bundle
Mouth 5, and tightened with the thread-locking ring 4 on basin, the closing insect prevention string bag 1 is reinforced, wood louse adult climbs on plant, raised into artificial
The stage of supporting.
Embodiment 3
(1) commercially available kamuning sapling is bought, is transplanted into food source plant Culture basin 2, potting soil is peat soil：Loess：Carefully
River sand=2:2:1.After drenching sufficient root water, food source plant Culture basin 2 is put in flower-pot cover 3, places more than 1 week slow seedling, it is right afterwards
Each branch of kamuning sapling carries out topping trimming once, and imposes liquid manure by flower-pot cover 3, and liquid manure is commercial liquid organic fertilizer and water
Dissolubility high-nitrogen compound fertilizer, (FUBON good fortune nation water soluble organic fertilizer：N-P-K content >=10%, the content of organic matter >=60%；YARA
Refined Ran high-N compound fertilizers：N:P:K=21:6:13, total nutrient content 40%), by 1:1 (w/w) ratio mixes, and is diluted to
5% (w/w) concentration, the liquid manure of this dilution is noted in flower-pot cover 3, acted on by self-priming, complete water and fertilizer management, unnecessary liquid manure
Discharged by the draining aperture of the side of flower-pot cover 3, be available for secondary use.Using inspiring young sprout after liquid manure, after young sprout grows out,
Cultivated available for wood louse.
(2) daily moisture management：
1. during potting soil arid, water can be injected in flower-pot cover 3, acted on by the self-priming of potting soil, complete moisture management, it is more
Remaining water is discharged by the draining aperture of the side of flower-pot cover 3.
2. used commercial liquid organic fertilizer and water-soluble high-nitrogen compound fertilizer, (FUBON good fortune nation water soluble organic fertilizer every 4 weeks：Nitrogen
Phosphorus potassium content >=10%, the content of organic matter >=60%；The refined Ran high-N compound fertilizers of YARA：N:P:K=21:6:13, total nutrient content
For 40%), by 1:1 (w/w) ratio is mixed, and is diluted to 5% (w/w) concentration, and liquid manure of this dilution is noted in flower-pot cover 3,
Acted on by self-priming, complete water and fertilizer management, unnecessary liquid manure is discharged by the draining aperture of the side bottom of flower-pot cover 3, is available for secondary
Use.
(3) wood louse nymph or ovum are gathered in orchard, i.e., the small stems for having wood louse ovum or nymph on fruit tree is cut, is put in anti-
In the worm string bag 1, laboratory is taken back.By this branch not in kamuning, putting the insect prevention string bag 1, beam lives tying 5, and with basin
Thread-locking ring 4 is tightened, and reinforces the closing insect prevention string bag 1, and wood louse nymph can be climbed in kamuning, start feeding, that is, is entered artificial
The raising stage.
Embodiment 4
(1) commercially available kamuning sapling is bought, transfer enters in food source plant Culture basin 2, and potting soil is peat soil：Loess：Carefully
River sand=2:2:1.After drenching sufficient root water, food source plant Culture basin 2 is put in flower-pot cover 3, places more than 1 week slow seedling, it is right afterwards
Each branch of kamuning sapling carries out topping trimming once, and imposes liquid manure by flower-pot cover 3, and liquid manure is commercial liquid organic fertilizer and water
Dissolubility high-nitrogen compound fertilizer, (FUBON good fortune nation water soluble organic fertilizer：N-P-K content >=10%, the content of organic matter >=60%；YARA
Refined Ran high-N compound fertilizers：N:P:K=21:6:13, total nutrient content 40%), by 1:1 (w/w) ratio mixes, and is diluted to
5% (w/w) concentration, the liquid manure of this dilution is noted in flower-pot cover 3, acted on by self-priming, complete water and fertilizer management, unnecessary liquid manure
Discharged by the draining aperture of the side of flower-pot cover 3, be available for secondary use.Using inspiring young sprout after liquid manure, after young sprout grows out,
Cultivated available for wood louse.
(2) daily moisture management：
1. during potting soil arid, water can be injected in flower-pot cover 3, acted on by the self-priming of potting soil, complete moisture management, it is more
Remaining water is discharged by the draining aperture of the side bottom of flower-pot cover 3.
2. daily nutrient management：Commercial liquid organic fertilizer and water-soluble high-nitrogen compound fertilizer (FUBON good fortune nation water were used every 4 weeks
Dissolubility organic fertilizer：N-P-K content >=10%, the content of organic matter >=60%；The refined Ran high-N compound fertilizers of YARA：N:P:K=21:6:
13, total nutrient content 40%), by 1:1 (w/w) ratio mixes, and is diluted to 5% (w/w) concentration, by the liquid manure of this dilution
To note in flower-pot cover 3, acted on by self-priming, complete water and fertilizer management, unnecessary liquid manure is discharged by the draining aperture of the side of flower-pot cover 3,
It is available for secondary use.
(3) orchard collection wood louse adult, and be positioned in the insect prevention string bag 1, beam lives tying 5, takes back laboratory, unties nylon
To restrict tying 5, be placed on to have planted and be implanted with the food source plant Culture basin 2 of kamuning, beam lives nylon rope tying 5, and with the screw thread on basin
Retaining ring 4 is tightened, and reinforces the closing insect prevention string bag 1, and wood louse adult is climbed on plant, into the artificial feeding stage.
(4) after raising a period of time, kamuning branch is gently patted, wood louse adult migrates onto the insect prevention string bag 1, turns on food
The thread-locking ring 4 of source plant Culture basin 2, unties the tying 5 of the insect prevention string bag 1, slowly lifts the insect prevention string bag 1, Bian Tibian is gently clapped
Kamuning branch is beaten, after departing from plant scope, rapid beam lives insect prevention string bag mouth, and tightens tying 5, and all wood louse adults are taken back
Laboratory research.
(5) the insect prevention string bag 1 again new on set on food source plant Culture basin 2, tightens tying 5, and tighten screw-threaded coupling
Ring 4, ovum or nymph in tender tip enter the breeding cycle of a new round.
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (10)
- A kind of 1. breeding apparatus of diaphorina citri, it is characterised in that：Including the insect prevention string bag, food source plant Culture basin and flower-pot cover, institute The insect prevention string bag stated is placed on the upper end open of food source plant Culture basin, and wood louse culturing room is collectively forming with food source plant Culture basin, Described food source plant Culture basin is placed in flower-pot cover.
- 2. the breeding apparatus of diaphorina citri according to claim 1, it is characterised in that：Described food source plant Culture basin is also Including thread-locking ring, described thread-locking ring is threadedly attached to the upper end open of food source plant Culture basin, simultaneously will The insect prevention string bag is fixed on food source plant Culture basin.
- 3. the breeding apparatus of diaphorina citri according to claim 1, it is characterised in that：The opening of the described insect prevention string bag It is additionally provided with tying.
- 4. the breeding apparatus of diaphorina citri according to claim 1, it is characterised in that：The described insect prevention string bag is 40~60 Mesh insect prevention mesh bag, its bore are more than the bore of food source plant Culture basin.
- 5. the breeding apparatus of diaphorina citri according to claim 1, it is characterised in that：Described food source plant Culture basin Bottom also includes the foot pad of projection；The side and bottom of described food source plant Culture basin are additionally provided with aperture；Described flower-pot cover Side also includes draining aperture and the plug for blocking draining aperture.
- 6. the breeding apparatus of diaphorina citri according to claim 1, it is characterised in that：Described food source plant Culture basin is Cylindrical food source plant Culture basin；Described flower-pot cover is that cylindrical type, diameter are bigger more than 2cm than the diameter of food source plant Culture basin Basin body, or it is more than base diameter, and the basin that base diameter is bigger more than 2cm than the diameter of food source plant Culture basin for opening diameter Body；The height of described flower-pot cover is half of food source plant Culture basin height or more.
- A kind of 7. method for breeding of diaphorina citri, it is characterised in that：Comprise the following steps：(1) the food source plant of diaphorina citri is planted in food source plant Culture basin, food source plant Culture basin is then placed in flower-pot cover In, liquid manure or water are noted in flower-pot cover, acted on by self-priming, completes rich water quality management, obtains food source plant；(2) diaphorina citri adult, nymph or ovum are gathered, is placed in the insect prevention string bag, kind in step (1) is placed on and is implanted with food source On the food source plant Culture basin of plant, beam lives tying, and tightens thread-locking ring, into the artificial feeding stage.
- 8. the method for breeding of diaphorina citri according to claim 7, it is characterised in that：Food source plant described in step (1) is kamuning or citrus；The potting soil in food source plant Culture basin described in step (1) is peat soil, loess and thin river sand by volume 2:2:1 Match obtained potting soil；Liquid manure described in step (1) is liquid organic fertilizer and water-soluble high-nitrogen compound fertilizer.
- 9. the method for breeding of diaphorina citri according to claim 8, it is characterised in that：Liquid manure described in step (1) is First by liquid organic fertilizer and water-soluble high-nitrogen compound fertilizer in mass ratio 1:1 proportioning mixing, then it is 5% (w/w's) to be diluted to concentration Liquid manure.
- 10. the method for breeding of the diaphorina citri according to any one of claim 7~9, it is characterised in that also include migration step Suddenly in (3) the step of diaphorina citri adult after artificial feeding：The branch of food source plant is patted, diaphorina citri adult is moved Fly on the insect prevention string bag, then turn on the thread-locking ring on food source plant Culture basin, untie the tying of the insect prevention string bag, then lift The insect prevention string bag, after departing from plant scope, beam lives insect prevention string bag mouth and tightens tying, and is covered again on food source plant Culture basin The new insect prevention string bag, tighten tying and tighten thread-locking ring, the ovum or nymph on branch enter the breeding cycle of next round.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110742021A (en) * | 2019-10-18 | 2020-02-04 | 福建省农业科学院水稻研究所 | Insect feeding and catching device and using method thereof |
| CN114794030A (en) * | 2022-06-16 | 2022-07-29 | 广东省农业科学院植物保护研究所 | Diaphorina citri drug resistance monitoring device and drug resistance determination method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104621052A (en) * | 2015-02-11 | 2015-05-20 | 广东省昆虫研究所 | Diaphorina citri Kuwayama parasitism natural energy scale breeding method |
| CN106561575A (en) * | 2016-10-19 | 2017-04-19 | 赣州市柑桔科学研究所 | Artificial feeding method for diaphorina citri |
| CN106954592A (en) * | 2017-04-14 | 2017-07-18 | 华南农业大学 | A kind of Ali eats the biological control method of lice encyrtid |
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2017
- 2017-12-13 CN CN201711323423.7A patent/CN107821340B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104621052A (en) * | 2015-02-11 | 2015-05-20 | 广东省昆虫研究所 | Diaphorina citri Kuwayama parasitism natural energy scale breeding method |
| CN106561575A (en) * | 2016-10-19 | 2017-04-19 | 赣州市柑桔科学研究所 | Artificial feeding method for diaphorina citri |
| CN106954592A (en) * | 2017-04-14 | 2017-07-18 | 华南农业大学 | A kind of Ali eats the biological control method of lice encyrtid |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110742021A (en) * | 2019-10-18 | 2020-02-04 | 福建省农业科学院水稻研究所 | Insect feeding and catching device and using method thereof |
| CN114794030A (en) * | 2022-06-16 | 2022-07-29 | 广东省农业科学院植物保护研究所 | Diaphorina citri drug resistance monitoring device and drug resistance determination method |
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