CN104222007B - A kind of artificial a large amount of methods producing parasitic wasp - Google Patents
A kind of artificial a large amount of methods producing parasitic wasp Download PDFInfo
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- CN104222007B CN104222007B CN201310254241.4A CN201310254241A CN104222007B CN 104222007 B CN104222007 B CN 104222007B CN 201310254241 A CN201310254241 A CN 201310254241A CN 104222007 B CN104222007 B CN 104222007B
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- 238000000034 method Methods 0.000 title claims abstract description 39
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- 238000004806 packaging method and process Methods 0.000 claims abstract description 14
- 241000594033 Liriomyza bryoniae Species 0.000 claims abstract description 12
- 241000594036 Liriomyza Species 0.000 claims abstract description 11
- 238000004519 manufacturing process Methods 0.000 claims abstract description 8
- 238000004064 recycling Methods 0.000 claims abstract description 5
- 241000196324 Embryophyta Species 0.000 claims description 69
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- 239000000463 material Substances 0.000 claims description 12
- 241000255925 Diptera Species 0.000 claims description 9
- 238000007789 sealing Methods 0.000 claims description 9
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- 241001481304 Vespoidea Species 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- 241000209094 Oryza Species 0.000 claims description 6
- 235000007164 Oryza sativa Nutrition 0.000 claims description 6
- 241000209140 Triticum Species 0.000 claims description 6
- 235000021307 Triticum Nutrition 0.000 claims description 6
- 230000009194 climbing Effects 0.000 claims description 6
- 235000013312 flour Nutrition 0.000 claims description 6
- -1 fly net Substances 0.000 claims description 6
- 235000009566 rice Nutrition 0.000 claims description 6
- 239000002023 wood Substances 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 5
- 102000002322 Egg Proteins Human genes 0.000 claims description 3
- 108010000912 Egg Proteins Proteins 0.000 claims description 3
- 241000238631 Hexapoda Species 0.000 claims description 3
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 claims description 3
- JEVVKJMRZMXFBT-XWDZUXABSA-N Lycophyll Natural products OC/C(=C/CC/C(=C\C=C\C(=C/C=C/C(=C\C=C\C=C(/C=C/C=C(\C=C\C=C(/CC/C=C(/CO)\C)\C)/C)\C)/C)\C)/C)/C JEVVKJMRZMXFBT-XWDZUXABSA-N 0.000 claims description 3
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- OAIJSZIZWZSQBC-GYZMGTAESA-N lycopene Chemical compound CC(C)=CCC\C(C)=C\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C=C(/C)CCC=C(C)C OAIJSZIZWZSQBC-GYZMGTAESA-N 0.000 claims description 3
- 229960004999 lycopene Drugs 0.000 claims description 3
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Abstract
The present invention relates to a kind of artificial a large amount of methods producing parasitic wasp, it is characterized in that raising liriomyza bryoniae, Liriomyza first with host plant, the prey that recycling is raised raises the method for parasitic wasp.The present invention provide not only the method for breeding of the method for breeding of prey, parasitic wasp, but also provides the method collecting parasitic wasp and the method for packaging parasitic wasp, and the process of planting matrix and recycling method.The present invention is that Liriomyza, commercially producing of liriomyza bryoniae parasitic wasp provide a kind of complete production technology first.
Description
Technical field
The present invention relates to a kind of artificial a large amount of methods producing parasitic wasp, belong to agricultural pests field of biological control.
Background technology
Liriomyza bryoniae and Liriomyza are two class important pests in agricultural production, and parasitic wasp is the Important Natural Enemy preventing and treating this two class pest.Although the commercial parasitic wasp of China has a lot, but liriomyza bryoniae parasitic wasp and Liriomyza parasitic wasp then not yet realize merchandized handling so far.
Although existing method for breeding is capable of liriomyza bryoniae parasitic wasp and the raising of Liriomyza parasitic wasp, but not only still in raising the stage on a small scale, and its a large amount of production procedures relevant need, lifting further is perfect.
Summary of the invention
It is an object of the invention to provide a kind of artificial a large amount of methods producing parasitic wasp, it is characterized in that raising prey first with host plant, described prey refers to liriomyza bryoniae and Liriomyza, two kinds of flies are individually raised or mix raising, the prey that recycling is raised raises the method for parasitic wasp, described parasitic wasp refers to liriomyza bryoniae parasitic wasp and Liriomyza parasitic wasp, and two kinds of parasitic wasps individually produce or mixture manufacturing, and concrete key operation step is:
1) inoculation of prey: host plant launches first in 10 days after true leaf, host plant is moved in prey transfer room together with its planting container, require that prey transfer room has enough sealings, it is able to ensure that prey will not fly out receptacle, access the ratio more than 2 in every strain host plant and access prey adult kind source, during the prey egg laying amount average out to more than 40 that every strain host plant contains, host plant and planting container thereof are transferred to prey receptacle from prey transfer room together, and move into new host plant to prey transfer room, host plant is changed once for every 0.5 day-3 days, prey adult kind source amount supplements new kind source when reducing in time.Wherein, described plantation utensil refers to for planting the basin of host plant, dish, container etc., and being filled with planting matrix in these utensils, host plant is grown in these planting matrixs.
2) raising of prey: giving the suitable humiture of host plant and moisture in prey receptacle, prey grows transfers to parasitic wasp transfer room by host plant together with planting container after the second age.
3) parasitic wasp inoculation: access parasitic wasp in the ratio of parasitic wasp with prey ovum 1: 5-1: 30, after 1 day, host plant and planting container thereof are transferred to parasitic wasp receptacle.
4) raising of parasitic wasp and collection: after receptacle internal excited state is raised 4-7 days, host plant is cut with shears, by plants stems, branch is put in collection device together with leaf, also can individually the blade of clip host plant or branch and leaf put in collection device, then collection device is sealed as early as possible, again collection device is placed in suitable space, fix the collection that namely can be used for parasitic wasp adult after putting well, collecting operation is every weekly check, collect more than 1 time, preferred every day checks, collect more than 1 time, till collecting pipe is collected less than parasitic wasp, collecting pipe is that length is within 1m, for collecting the container of parasitic wasp, guarantee to collect less than the plants stems that can clear up in collection device after parasitic wasp, branch or leaf.
Wherein, described collection device refers to the utensil for collecting parasitic wasp, collection device can be made up of two parts and also can be made up of three parts, preferred collection device is made up of three parts, if collection device is made up of two parts, including collecting container and collecting pipe two parts, when collecting parasitic wasp, directly and coated lycopene, parasitic wasp is directly to climb in collecting pipe from collecting in container to collecting pipe；If collection device is made up of three parts, including collecting container, being contained in the collecting duct and collecting pipe three part collected on container, when collecting parasitic wasp, collecting pipe is directly connected with collecting duct, and parasitic wasp is first to climb in collecting duct to climb in collecting pipe in collection container again.
Described collection device is placed on suitable space and refers to the space being designed for parasitic wasp collection, require that this space has light unglazed when parasitic wasp is collected, but need light in fixing collection device, inspection when changing the plants stems in collecting pipe, cleaning collection device, branch or leaf.
Described collection container refers to for loading parasitic wasp and the part of plants stems, branch or leaf, collects container and has 0.001m3-1m3Volume, the manufacture material collecting container must contain gauze, gauze, cloth, fly net, paper or other material can breathed freely and can effectively stop parasitic wasp to escape, described gauze, gauze, cloth, fly net, paper or other can be breathed freely and but the material that can effectively stop parasitic wasp to escape can be used alone also two or more mixing and uses, collect container and must also have good sealing, parasitic wasp can be ensured as far as possible cannot fly out from other place except climbing to or fly to except in collecting pipe or climb out of, collect container and also there is the opening being easily opened and sealing, open opening and can load parasitic wasp and plants stems, branch or leaf, seal opening and salable collection container.
Described collecting duct refers to be contained in collects the passage flying out for parasitic wasp on container or climbing out of, parasitic wasp can be contained in that collect container and flies out or climb out of collection container from collecting duct, that connecting together from collecting duct and collecting pipe again flies into or crawls into collecting pipe, the collecting duct number collected on container is one or more, and the length of collecting duct is within 1m.
5) packing method of parasitic wasp: after utilizing collection device to collect parasitic wasp, by packaging standard, parasitic wasp adult is put into the Packaging Bottle with Vermiculitum, wood flour, wheat husk or rice husk, then bottle cap is quickly covered tightly, Packaging Bottle body and bottle cap can fully seal, can effectively stop parasitic wasp escape, parasitic wasp can be applied after having packed or continue cold preservation, described Packaging Bottle bottle cap is with air-vent, air-vent size, shape are not fixed, but air-vent need to be sealed by paper or gauze, gauze, cloth, it is preferable that air-vent is sealed by paper.Wherein said Vermiculitum, wood flour, wheat husk or rice husk refer to the package media of parasitic wasp, and various package medias can be used alone, it is possible to two kinds and use mixed above.
null6) process of planting matrix and re-using: first remove used planting matrix in parasitic wasp receptacle，Place more than 0.1 day，It is continuing with after directly sieving or smash with pulverizer after planting matrix certain mass dryness fraction to be achieved，If wherein sieving, screen sizes is 0.1cm-10cm，Screen cloth can allow planting matrix to pass through and can prevent host plant root、Stem、Leaf or other foreign material pass through，The root, stem and leaf of host plant can be continuing with after smashing in pulverizer or not in use by，The planting matrix stayed after sieving or after smashing can directly utilize or mixes use with new planting matrix or add other composition being conducive to host plant to grow，But preferably through freezing before utilizing these used planting matrixs、High temperature deinsectization sterilizing or process of being exposed to the sun，To guarantee not have in planting matrix parasitic wasp、Prey or other insects、Pathogenic bacterium etc..
The present invention provide not only the method for breeding of liriomyza bryoniae, the method for breeding of Liriomyza, parasitic wasp, but also provides the method collecting parasitic wasp and the method for packaging parasitic wasp, and the process of planting matrix and recycling method.The present invention is that Liriomyza, commercially producing of liriomyza bryoniae parasitic wasp provide a kind of complete production technology first, it is possible to the Biological control for liriomyza bryoniae class and leaf mining flies provides substantial amounts of nature enemy in amount.
Detailed description of the invention
Following methods is only that the embodiment to claim and summary of the invention is illustrated, and does not represent the restriction to present claims and summary of the invention.
The inoculation of embodiment 1 prey: host plant launches first in 1 day after true leaf, host plant is moved in prey transfer room together with its planting container, require that prey transfer room has enough sealings, it is able to ensure that prey will not fly out receptacle, access the ratio more than 6 in every strain host plant and access prey adult kind source, during the prey egg laying amount average out to more than 80 that every strain host plant contains, host plant and planting container thereof are transferred to prey receptacle from prey transfer room together, and move into new host plant to prey transfer room, host plant is changed once for every 1 day, prey adult kind source amount supplements new kind source when reducing in time.Wherein, described plantation utensil refers to for planting the basin of host plant, dish, container etc., and being filled with planting matrix in these utensils, host plant is grown in these planting matrixs.
The raising of embodiment 2 prey: giving the suitable humiture of host plant and moisture in prey receptacle, prey grows transfers to parasitic wasp transfer room by host plant together with planting container after the second age.
Embodiment 3 parasitic wasp inoculates: accesses parasitic wasp in the ratio of parasitic wasp with prey ovum 1: 15, after 1 day, host plant and planting container thereof is transferred to parasitic wasp receptacle.
The raising of embodiment 4 parasitic wasp and collection: after receptacle internal excited state is raised 5 days, host plant is cut with shears, by plants stems, branch is put in collection device together with leaf, also can individually the blade of clip host plant or branch and leaf put in collection device, then collection device is sealed as early as possible, again collection device is placed in suitable space, fix the collection that namely can be used for parasitic wasp adult after putting well, collecting operation is every weekly check, collect more than 1 time, preferred every day checks, collect more than 1 time, till collecting pipe is collected less than parasitic wasp, collecting pipe is that length is within 1m, for collecting the container of parasitic wasp, guarantee to collect less than the plants stems that can clear up in collection device after parasitic wasp, branch or leaf.
Wherein, described collection device refers to the utensil for collecting parasitic wasp, collection device can be made up of two parts and also can be made up of three parts, preferred collection device is made up of three parts, if collection device is made up of two parts, including collecting container and collecting pipe two parts, when collecting parasitic wasp, directly and coated lycopene, parasitic wasp is directly to climb in collecting pipe from collecting in container to collecting pipe；If collection device is made up of three parts, including collecting container, being contained in the collecting duct and collecting pipe three part collected on container, when collecting parasitic wasp, collecting pipe is directly connected with collecting duct, and parasitic wasp is first to climb in collecting duct to climb in collecting pipe in collection container again.
Described collection device is placed on suitable space and refers to the space being designed for parasitic wasp collection, require that this space has light unglazed when parasitic wasp is collected, but need light in fixing collection device, inspection when changing the plants stems in collecting pipe, cleaning collection device, branch or leaf.
Described collection container refers to for loading parasitic wasp and the part of plants stems, branch or leaf, collects container and has 0.001m3-1m3Volume, the manufacture material collecting container must contain gauze, gauze, cloth, fly net, paper or other material can breathed freely and can effectively stop parasitic wasp to escape, described gauze, gauze, cloth, fly net, paper or other can be breathed freely and but the material that can effectively stop parasitic wasp to escape can be used alone also two or more mixing and uses, collect container and must also have good sealing, parasitic wasp can be ensured as far as possible cannot fly out from other place except climbing to or fly to except in collecting pipe or climb out of, collect container and also there is the opening being easily opened and sealing, open opening and can load parasitic wasp and plants stems, branch or leaf, seal opening and salable collection container.
Described collecting duct refers to be contained in collects the passage flying out for parasitic wasp on container or climbing out of, parasitic wasp can be contained in that collect container and flies out or climb out of collection container from collecting duct, that connecting together from collecting duct and collecting pipe again flies into or crawls into collecting pipe, the collecting duct number collected on container is one or more, and the length of collecting duct is within 1m.
The packing method of embodiment 5 parasitic wasp: after utilizing collection device to collect parasitic wasp, by packaging standard, parasitic wasp adult is put into the Packaging Bottle with Vermiculitum, wood flour, wheat husk or rice husk, then bottle cap is quickly covered tightly, Packaging Bottle body and bottle cap can fully seal, can effectively stop parasitic wasp escape, parasitic wasp can be applied after having packed or continue cold preservation, described Packaging Bottle bottle cap is with air-vent, air-vent size, shape are not fixed, but air-vent need to be sealed by paper or gauze, gauze, cloth, it is preferable that air-vent is sealed by paper.Wherein said Vermiculitum, wood flour, wheat husk or rice husk refer to the package media of parasitic wasp, and various package medias can be used alone, it is possible to two kinds and use mixed above.
nullThe process of embodiment 6 planting matrix and re-using: first in parasitic wasp receptacle, remove used planting matrix，Place more than 0.1 day，It is continuing with after directly sieving or smash with pulverizer after planting matrix certain mass dryness fraction to be achieved，If wherein sieving, screen sizes is 0.1cm-10cm，Screen cloth can allow planting matrix to pass through and can prevent host plant root、Stem、Leaf or other foreign material pass through，The root, stem and leaf of host plant can be continuing with after smashing in pulverizer or not in use by，The planting matrix stayed after sieving or after smashing can directly utilize or mixes use with new planting matrix or add other composition being conducive to host plant to grow，But preferably through freezing before utilizing these used planting matrixs、High temperature deinsectization sterilizing or process of being exposed to the sun，To guarantee not have in planting matrix parasitic wasp、Prey or other insects、Pathogenic bacterium etc..
Claims (7)
1. artificial a large amount of methods producing parasitic wasp, it is characterized in that raising prey first with host plant, described prey refers to liriomyza bryoniae and Liriomyza, two kinds of flies are individually raised or mix raising, the prey that recycling is raised raises the method for parasitic wasp, described parasitic wasp refers to liriomyza bryoniae parasitic wasp and Liriomyza parasitic wasp, and two kinds of parasitic wasps individually produce or mixture manufacturing, and concrete key operation step is:
1) inoculation of prey: host plant launches first in 10 days after true leaf, host plant is moved in prey transfer room together with its plantation utensil, require that prey transfer room has enough sealings, it is able to ensure that prey will not fly out receptacle, access the ratio more than 2 in every strain host plant and access prey adult kind source, during the prey egg laying amount average out to more than 40 that every strain host plant contains, host plant and plantation utensil thereof are transferred to prey receptacle from prey transfer room together, and move into new host plant to prey transfer room, host plant is changed once for every 0.5 day-3 days, prey adult kind source amount supplements new kind source when reducing in time；
2) raising of prey: give the suitable humiture of host plant and moisture in prey receptacle, prey grew after the second age transfers to parasitic wasp transfer room by host plant together with plantation utensil；
3) parasitic wasp inoculation: access parasitic wasp in the ratio of parasitic wasp with prey ovum 1: 5-1: 30, transferred to parasitic wasp receptacle by host plant and plantation utensil thereof after 1 day；If raising parasitic wasp continuously, then need continuously the host plant with prey to be transferred to parasitic wasp transfer room；
4) raising of parasitic wasp and collection: when receptacle internal excited state is raised and not yet sprouted wings for 4-10 days, host plant is cut with shears, by plants stems, branch is put in collection device together with leaf, or individually the blade of clip host plant or branch and leaf are put in collection device, then collection device is sealed as early as possible, again collection device is placed in suitable space, fix the collection that namely can be used for parasitic wasp adult after putting well, collecting operation is every weekly check, collect more than 1 time, till collecting pipe is collected less than parasitic wasp, collecting pipe is that length is within 1m, for collecting the container of parasitic wasp, guarantee to collect less than the plants stems that can clear up in collection device after parasitic wasp, branch or leaf；
5) packing method of parasitic wasp: after utilizing collection device to collect parasitic wasp, by packaging standard, parasitic wasp adult is put into the Packaging Bottle with Vermiculitum, wood flour, wheat husk or rice husk, then bottle cap is quickly covered tightly, Packaging Bottle body and bottle cap can fully seal, can effectively stop parasitic wasp escape, parasitic wasp can be applied after having packed or continue cold preservation, described Packaging Bottle bottle cap is with air-vent, and air-vent size, shape are not fixed, but air-vent need to be sealed by paper or gauze, cloth；
null6) process of planting matrix and re-using: first remove used planting matrix in parasitic wasp receptacle，Place more than 0.1 day，It is continuing with after directly sieving or smash with pulverizer after planting matrix certain mass dryness fraction to be achieved，If wherein sieving, screen sizes is 0.1cm-10cm，Screen cloth can allow planting matrix to pass through and can prevent host plant root、Stem、Leaf or other foreign material pass through，The root, stem and leaf of host plant be continuing with after smashing in pulverizer or not in use by，The planting matrix stayed after sieving or after smashing directly utilizes or mixes use with new planting matrix or add other composition being conducive to host plant to grow，But before utilizing these used planting matrixs, to pass through freezing、High temperature deinsectization sterilizing or process of being exposed to the sun，To guarantee not have in planting matrix parasitic wasp、Prey or other insects、Pathogenic bacterium.
2. a kind of artificial a large amount of methods producing parasitic wasps according to claim 1, is characterized in that described plantation utensil refers to the container for planting host plant, are filled with planting matrix in these utensils, host plant is grown in these planting matrixs.
3. a kind of artificial a large amount of methods producing parasitic wasps according to claim 1, it is characterized in that described collection device refers to the utensil for collecting parasitic wasp, collection device is made up of two parts or is made up of three parts, if collection device is made up of two parts, including collecting container and collecting pipe two parts, when collecting parasitic wasp, directly and coated lycopene, parasitic wasp is directly to climb in collecting pipe from collecting in container to collecting pipe；If collection device is made up of three parts, including collecting container, being contained in the collecting duct and collecting pipe three part collected on container, when collecting parasitic wasp, collecting pipe is directly connected with collecting duct, and parasitic wasp is first to climb in collecting duct to climb in collecting pipe in collection container again.
4. a kind of artificial a large amount of methods producing parasitic wasps according to claim 1, it is characterized in that described collection device is placed on suitable space and refers to the space being designed for parasitic wasp collection, require that this space has light unglazed when parasitic wasp is collected, but need light in fixing collection device, inspection when changing the plants stems in collecting pipe, cleaning collection device, branch or leaf.
5. a kind of artificial a large amount of methods producing parasitic wasps according to claim 1, is characterized in that described Vermiculitum, wood flour, wheat husk or rice husk refer to the package media of parasitic wasp, and various package medias are used alone, or two or more mixing uses.
6. a kind of artificial a large amount of methods producing parasitic wasps according to claim 3, is characterized in that described collection container refers to for loading parasitic wasp and the part of plants stems, branch or leaf, collect container and have 0.001m3-1m3Volume, the manufacture material collecting container must contain gauze, cloth, fly net, paper or other material can breathed freely and can effectively stop parasitic wasp to escape, described gauze, cloth, fly net, paper or other can be breathed freely and the material that can effectively stop parasitic wasp to escape is used alone or two or more mixing uses, collect container and must also have good sealing, parasitic wasp can be ensured as far as possible cannot fly out from other place except climbing to or fly to except in collecting pipe or climb out of, collect container and also there is the opening being easily opened and sealing, open opening and can load parasitic wasp and plants stems, branch or leaf, seal opening and salable collection container.
7. a kind of artificial a large amount of methods producing parasitic wasps according to claim 3, it is characterized in that described collecting duct refers to be contained in and collect the passage flying out for parasitic wasp on container or climbing out of, parasitic wasp is contained in that collect container and flies out or climb out of collection container from collecting duct, that connecting together from collecting duct and collecting pipe again flies into or crawls into collecting pipe, the collecting duct number collected on container is one or more, and the length of collecting duct is within 1m.
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- 2013-06-21 CN CN201310254241.4A patent/CN104222007B8/en active Active
Patent Citations (5)
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|---|---|---|---|---|
| CN101904320A (en) * | 2010-07-27 | 2010-12-08 | 中山大学 | Method for breeding a large number of microplitis bicoloratus |
| CN102144619A (en) * | 2011-04-13 | 2011-08-10 | 云南省热带作物科学研究所 | Artificial propagation method of diversinervus elegans silvestri |
| CN102578052A (en) * | 2012-03-16 | 2012-07-18 | 宁波市林特科技推广中心 | A bayberry orchard Drosophila elanogaster artificial breeding method, and method for breeding parasitic wasp with the same |
| CN102630647A (en) * | 2012-05-03 | 2012-08-15 | 中国农业科学院植物保护研究所 | Method for propagating Diglyphus isaea |
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| CN104222007B8 (en) | 2016-12-07 |
| CN104222007A (en) | 2014-12-24 |
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