CN102630647A - Method for propagating Diglyphus isaea - Google Patents
Method for propagating Diglyphus isaea Download PDFInfo
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- CN102630647A CN102630647A CN2012101355967A CN201210135596A CN102630647A CN 102630647 A CN102630647 A CN 102630647A CN 2012101355967 A CN2012101355967 A CN 2012101355967A CN 201210135596 A CN201210135596 A CN 201210135596A CN 102630647 A CN102630647 A CN 102630647A
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Abstract
The invention discloses a method for propagating Diglyphus isaea. The method provided by the invention comprises the following steps: 1) putting plant seedling with host larvae of Diglyphus isaea into an inset feeding container, introducing female Diglyphus isaea about to spawn, and after the oviposition into the body of the host larvae, cultivating till the host larvae develop into pupae so as to obtain the plant A with the pupae; and 2) taking out the plant A with the pupae for Diglyphus isaea culture and collecting Diglyphus isaea so as to realize propagation. The experiment shows that technical sections such as Diglyphus isaea reproduction host selection, the optimal Diglyphus isaea inoculation ratio, the key period of Diglyphus isaea inoculation, the collection and storage of Diglyphus isaea imagoes and the like are subjected to repeated exploration and improvement to form an efficient, economic and simple propagation technology of parasitic Diglyphus isaea; Diglyphus isaea can be propagated on a large scale rapidly, and the purpose of preventing harmful insects is reached.
Description
Technical field
The present invention relates to biological technical field, relate in particular to a kind of method that expands the latent fly Ji chalcid fly of numerous pea.
Background technology
Liriomyza bryoniae is the insect that one type of serious harm vegetables produces, and mainly comprises Americal rice leaf miner, south american leaf miner and clover liriomyza bryoniae etc.Harm protection ground and booth vegetable wherein are injured the heaviest with pulse family, Solanaceae and Curcurbitaceae.The imago feeding water of this class pest causes gets the food hole and ovum is originated in the blade, and the larva after the hatching is got food mesophyll in blade, can on blade, cause a lot of mines, and the epidermis of breaking by the teeth mostly after larva is aging gets out blade or the soil table that drops is pupated.Severe impairment is not only caused to some ornamental plants in larva mine and imago feeding hole, and influences photosynthesis of plant largely, causes the vegetables blade withered, and output reduces, quality descends, even whole strain is dead.
The good parasitic wasp that the latent fly Ji chalcid fly of pea is the parasitic multiple Liriomyza class pest of convertibility is used this parasitic wasp control Liriomyza and has lot of advantages, the most important thing is ability instead of chemical agricultural chemicals, reaches the purpose of prevention and control Liriomyza harm, the production green agricultural product.In addition, this parasitic wasp also has other advantages, mainly comprises: (1) it applicable to various crop (Kidney bean, pea, rape, tomato, cucumber etc.); (2) it can parasitic multiple Liriomyza (Americal rice leaf miner, Americal rice leaf miner, south american leaf miner, clover liriomyza bryoniae etc.); (3) its developmental rate is faster than Liriomyza, and population can increase and grow surely apace; (4) its pest control effect is good, can stop quickly and efficiently causing harm of Liriomyza larva; (5) its pupa and adult can both be easy to observe with the naked eye in the field; (6) it can not only parasitic Liriomyza larva, can also suck low instar larvae.
Therefore; The pea fly Ji chalcid fly (Diglyphus isaea Walker) of diving is the good parasitic wasp of prevention and control liriomyza bryoniae; Can in pest body, lay eggs, grow in pest body the hatching back, kills off the insect pests thereby draw insect nutrition; In addition, the one-tenth honeybee of this parasitic wasp can also directly be stung the suction insect and cause its death.In meridional many countries, no matter in the greenhouse or the field, the pea fly Ji chalcid fly of diving all is the important parasitic wasp of Liriomyza on vegetables and the ornamental plants.In countries such as Russia, Italy, Israel, Greece, the pea fly Ji chalcid fly of diving all plays important control action to cause harm serious south american leaf miner, clover liriomyza bryoniae, tomato liriomyza bryoniae of locality.
In view of the good biological and ecological methods to prevent plant disease, pests, and erosion ability of this parasitic wasp, sum up the efficient multiplication technique of screening, produce the natural enemy insect product in a large number, be significant for the Liriomyza class pest in prevention and control China vegetables, gardening, agricultural, the forestry.China starts late to the research work of parasitic wasps such as the latent fly Ji chalcid fly of pea.1996, China Agricultural Sciences Academy Biological Control Institute introduced the latent fly Ji chalcid fly of pea from Holland first and prevents and treats Americal rice leaf miner, and the laboratory test control efficiency is better.
Summary of the invention
An object of the present invention is to provide a kind of method that expands the latent fly Ji chalcid fly of numerous pea.
Method provided by the invention comprises the steps:
The plant seedlings that 1) will have the latent fly Ji chalcid fly host larva of pea is put into foster worm container, inserts the latent fly Ji chalcid fly queen bee of pea to be laid eggs, and being cultured to said host's larvae development after in said host larva body, laying eggs is the pupa shape, obtains having the plant A of pupa;
2) take out the said plant A that has pupa and go out the honeybee cultivation, collect the latent fly Ji chalcid fly of pea, realize expanding numerous.
In the said method, in the step 1), the latent fly Ji chalcid fly host of said pea is an Americal rice leaf miner,
The latent fly Ji chalcid fly host larva of said pea is the 2-3 Americal rice leaf miner in age in week.
Said access pea is dived fly Ji chalcid fly for inserting the latent fly Ji chalcid fly queen bee of pea.
In the said method, in the step 1), the number ratio of latent fly Ji chalcid fly queen bee of said access pea and the latent fly Ji chalcid fly host larva of said pea is 1: (15-25).
In the said method, in the step 1), the number of latent fly Ji chalcid fly queen bee of said access pea and the latent fly Ji chalcid fly host larva of said pea is than being 1:25.
In the said method, in the step 1), said plant is a leguminous plant, and said leguminous plant is specially Kidney bean;
Said plant seedlings is specially 13-17 days Kidney bean seedling of growth; Said Kidney bean seedling is specially 13 days Kidney bean seedling of growth; 13 days Kidney bean seedling of said growth is that phaseolus vulgaris seeds is after planting cultivated the Kidney bean seedling that obtained in 13 days, does the 1st day from the planting seed note.
The true leaf area of 13 days Kidney bean seedling of said growth is 0.01-0.02m
2
In the said method, step 2) in, saidly goes out honeybee and cultivate to the blade of the said plant A that will take out and in going out the honeybee container, cultivated 3-6 days.
In the said method, step 2) in, saidly goes out honeybee and cultivate to the blade of the said plant A that will take out and in going out the honeybee container, cultivated 3-6 days.
In the said method; Before step 1); Comprise that also preparation has the step of the plant seedlings of the latent fly Ji chalcid fly host larva of pea: in the foster worm container that is placed with 13 days Kidney bean seedling of said growth, insert paired Americal rice leaf miner; Lay eggs, cultivate, obtain having the plant seedlings of the latent fly Ji chalcid fly host larva of pea.
In the said method, said preparation has pea dives in the step of plant seedlings of fly Ji chalcid fly host larva, and said Americal rice leaf miner connects 250 pairs of Americal rice leaf miners according to every square metre of said foster worm container and inserts.
Also can insert according to 25 pairs of Americal rice leaf miners of 13 days Kidney bean seedling of the said growth of every strain inoculation.
In the said method, said preparation has pea dives in the step of plant seedlings of fly Ji chalcid fly host larva, and said cultivation is cultivated 2 week-3 weeks of ovum for removing said Americal rice leaf miner at the said back 2h of laying eggs, from laying eggs day note work the 1st day.
In the said method, step 2) in, the latent fly Ji chalcid fly of said collection pea is following A or B:
A, will in water, soak 10-15 minute, and shake simultaneously, collect said pupa, treat that said pupa sprouts wings, obtain the latent fly Ji chalcid fly of pea with the said pupa that comes off through the Kidney bean seedling leaves that has pupa that goes out after honeybee is cultivated;
B, the container that will place openend to seal through the Kidney bean seedling leaves that has pupa that goes out after honeybee is cultivated; And with said container place the collection beehive; Above said collection beehive, light is provided, after treating to dive said pea the little bee pupa of a fly Ji sprouting wings, opens the opening of said container; Utilize the latent fly Ji chalcid fly of said pea to become the phototaxis of honeybee, the latent fly Ji chalcid fly one-tenth of said pea honeybee gets into the completion of collection beehive automatically and collects.
In the said method, 13 days Kidney bean seedling of said growth prepares according to the method that comprises following A, B and C step:
A, with the planting seed of Kidney bean, cultivate, obtain the Kidney bean seedling;
The time of said cultivation was specially 5 days, will sow diary and do the 1st day;
B, with said Kidney bean seedling replanting in the nutritive cube that contains culture matrix, continue to cultivate, obtain having the seedling of true leaf;
Above-mentioned said seedling replanting is specially according to 2 said seedling replantings of every alms bowl in the nutritive cube that contains culture matrix,
Above-mentioned culture matrix is specially the mixture that the peat composed of rotten mosses and vermiculite mix with volume ratio 2 ︰ 1;
The time that said continuation is cultivated was specially 3 days;
C, the said growth of seedlings point that has true leaf of removal are cultivated once more, obtain 13 days Kidney bean seedling of said growth;
The said time of cultivating once more is 5 days;
The condition of various said cultivations all is specially 27 ± 0.5 ℃ of temperature, relative moisture 65%-70%, photoperiod L: D=14h: 10h.
Experiment of the present invention proves; The present invention connects the honeybee ratio, connects honeybee crucial opportunity, becomes sport technique segment such as honeybee collection storage around numerous honeybee host screening, the best; Through exploring repeatedly and improving, formed efficient, economic, easy parasitic wasp multiplication technique, can expand the latent fly Ji chalcid fly of numerous pea fast in a large number; Reach the purpose that the insect biology is prevented, concrete advantage is following:
(1) with low cost: the kind seedling and propagating link that technology adopts, Kidney bean expands numerous matrix (vermiculite) can repeatedly use for kind commonly used, only needs to get final product through solar exposure.The leaf nutrition that carries the fly larva of diving comes from the cotyledon of Kidney bean, mustn't extra additional fertilizer and nutrient solution.The nutritive cube that other each links adopt, gauze etc. all are low value article, are prone to buy also and can process voluntarily.
(2) space availability ratio height: present technique is not limit and is expanded numerous space, to the production line that expands numerous natural enemy, little of easy greenhouse and plastic greenhouse, all can be used to expand the latent fly Ji chalcid fly natural enemy product of numerous pea greatly.
(3) be convenient to the batch production operation: present technique relates to each link that expands the latent fly Ji chalcid fly of numerous pea, and batch production production is convenient in the operation that all can intensify.
(4) pupa and become honeybee to collect automatically, and can be respectively collect the pupa card to the difference of prevention and control object, become honeybee, rapid release is saved a large amount of artificial material resources.
Embodiment
Employed experimental technique is conventional method like no specified otherwise among the following embodiment.
Used material, reagent etc. like no specified otherwise, all can obtain from commercial sources among the following embodiment.
Embodiment 1, the latent fly Ji chalcid fly of the numerous pea of expansion
One, the cultivation of seedling
1, the seedling kind is selected
Seedling is selected Kidney bean, and kind is the English red kidney bean, select color just, big, full, the regular shape of particle, the uniform seed of quality.
2, seedling is numerous soon
Quick propagating and cultivating condition: the optimal environmental condition of seedling plantation growth: 27 ± 0.5 ℃ of temperature, relative moisture 65%-70%, photoperiod L: D=14h: 10h.
1) vernalization sowing: the seed cultivation in that sealing is isolated is indoor, (puts into vermiculite in the 60cm * 30cm), is about 2/3 of basin volume in the vinyl disc alms bowl; With vermiculite wetting evenly (must guard against water can not be too much, prevents that Kidney bean from rotting), then phaseolus vulgaris seeds is pressed in the vermiculite; Just do not had vermiculite to get final product; Cultivate about 5d, obtain seed germination and the stem length bean seedlings to about the 3cm, will sow diary and do the 1st day.
2) bean seedlings that and the time-division alms bowl: with above-mentioned 1) obtain move on in the nutritive cube that diameter is 12cm, and with composite soil (peat composed of rotten mosses and vermiculite are with 2 ︰, 1 mixing) cultivation, 2 in every alms bowl continues to cultivate 3 days, longly fully launch to 2 true leaves.
3) two leaf carriers connect worm: when bean seedlings length to 2 true leaf fully launches; Pinch to remove growing point, make intraseminal follow-up nutrition and photosynthesis assimilation nutrition this two true sheets that all are used for growing, cultivated once more 5 days; Obtain the bean seedlings of growing 13 days, with 2) cultivate and finish diary and do the 0th day.
Cultivate 13d from seed altogether to obtaining needed seedling, the true leaf area fully is extended to 0.01-0.02m
2The time, both can be used for next link.
Two, inoculation (meeting the host)
1, the host selects:
Select pea to dive fly Ji chalcid fly advantage parasitized host---Americal rice leaf miner (Americal rice leaf miner field reagent screening test Xiao spring thunder Luo Feng Zhou Binyang gift wise man's Liu Yong Kong Xiangyi " the Changjiang river vegetables " the 20th phase in 2011; The public can obtain from plant protection research institute of the Chinese Academy of Agricultural Sciences), with its larva as numerous honeybee host.
2, the guarantor plants and keeps: promptly the guarantor plants the foundation of population and keeps;
Expand numerous demand for satisfying, need set up the guarantor population of the latent fly Ji chalcid fly of Americal rice leaf miner and pea, the suitable guarantor of this two population plants environmental condition and is: 26 ± 0.5 ℃ of temperature, relative moisture RH65%-70%, photoperiod L: D=14h: 10h.Regularly carry out rejuvenation from field acquisition original inhabitants population.
3, inoculation
1) selection of inoculation time
Test method: respectively 9d, 11d, 13d, 15d, 17d (are sowed the vegetative period day its which sky was several days vegetative period certainly; The sowing diary is done the 1st day) each 1 alms bowl of Kidney bean move into and connect in the worm cage; And in cage, insert 30 pairs of Americal rice leaf miner adults; To connect the worm cage then and place (26 ℃ of temperature, relative moisture RH70%, photoperiod L: D=14h: 10h in the climatic cabinate.）。Americal rice leaf miner fly adult behind the 2h of laying eggs on the Kidney bean with its rejecting.To have ovum each in vegetative period Kidney bean at climatic cabinate (26 ℃ of temperature, relative moisture RH70%, photoperiod L: D=14h: continue 10h) to cultivate.Treat the ovum hatching on the Kidney bean blade and observe larva number and total larva number of unit are on each blade in larvae development during to 1 age, relatively Americal rice leaf miner is to the palatability of different growing stages Kidney bean.Repeat for totally 5 times.
The result is as shown in table 1, and Americal rice leaf miner is to the selective difference of Kidney bean seedling of different growing stages, and all in all, Americal rice leaf miner is more liked and got the aged partially blade of food, and average total larva number average is more than 38 on the Kidney bean blade of 13d, 15d, 17d.Wherein, Americal rice leaf miner is to being vegetative period that the Kidney bean seedling selectivity of 13d is the strongest, and total larva number can reach 112 at most on the blade, and unit are larva number is 0.39/cm
2Selectivity there was no significant difference to the Kidney bean in other each vegetative period.Therefore, to have a liking for food most be the Kidney bean seedling of 13d vegetative period to Americal rice leaf miner.
Table 1 Americal rice leaf miner is to the selectivity of different growing stages Kidney bean in vegetative period
Annotate: data are mean+SD in the table; Different lowercases are illustrated in the significance of difference on 0.05 level
Therefore, (the true leaf area fully is extended to 0.01-0.02m to select for use the Kidney bean in 13d vegetative period not have the worm seedling
2) move into and to connect the zooecium inoculation, this moment, broad bean blade area and tender degree were best, and 110 of worms can be born in the monolithic blade face, and unit are larva number is 0.39/cm
2, be best inoculation time.
2) connect the selection that the host measures
Test method: each connects worm cage (50 * 40 * 40) and puts into the Kidney bean seedling that be 13d vegetative period; Totally 4 processing; And in 4 cages, insert 10 pairs, 30 pairs, 50 pairs, 70 pairs Americal rice leaf miner adults respectively and let it get food, lay eggs, reject adult behind the 2h of laying eggs, and the Kidney bean seedling that will have an ovum shifts out and connects the worm cage and place climatic cabinate (26 ℃ of temperature; Relative moisture RH70%, photoperiod L: D=14h: continue 10h) to cultivate for 3 weeks.
Treat the ovum hatching on the Kidney bean blade and observe larva number and total larva number of unit are on each blade during to 3 ages in larvae development.Every processing repeats for 5 times.
Result of the test is as shown in table 2, can find out, when inserting 70 pairs of Americal rice leaf miner adults, because insect density is too high, between each instar larvae of Americal rice leaf miner, has fierce intraspecific competition, and a part of larva fails to grow to 3 ages just dead; Growth to 3 is during age because the larval feeding amount is bigger, cause the Kidney bean blade full flower, mesophyll food to the greatest extent, surplus epidermal tissue up and down only, whole blade is withered even shrivelled.When inserting 50 pairs of Americal rice leaf miner adults, total larva number, single leaf larva number, unit are larva number can reach 112.6,56.7,0.83/cm respectively
2, can avoid intraspecific competition, can obtain again a large amount of healthy 3 age the Americal rice leaf miner larva.
Therefore, the righttest quantity that every cage inserts the Americal rice leaf miner adult is 50 pairs, converts to meeting 250 couples/m of unit are in the zooecium
2Density (the dependent insect cage floor space is 45cm*45cm=0.2025m
2, can place 5 dependent insect cages for every square metre, 50 pairs in every cage is 250 couples/m so connect the borer population amount
2), 50 pairs in every alms bowl, every alms bowl 2 strains.
The righttest borer population amount that connects of table 2 Americal rice leaf miner
Annotate: data are mean+SD in the table; Different lowercases are illustrated in the significance of difference on 0.05 level in the table
Therefore, by 250 couples/m
2Density in cage, insert the Americal rice leaf miner adult, it is laid eggs on Kidney bean, be the inoculum concentration of the best.At this moment, the total larva number, single leaf larva number, the unit are larva number that expand in numerous cage can reach 112.6,56.7,0.83/cm respectively
2, can avoid intraspecific competition, can obtain a large amount of 3 healthy Americal rice leaf miner larvas in age (being present on the Kidney bean seedling) again.
4, batch expands numerous
Cultivate seedling as stated above in 2 weeks of every interval, saturatedly connect worm,, satisfy the prevention and control requirement, guarantee that product supplies with its batch production that realizes relay property.
Three, numerous honeybee
1, meets the test method and the result of honeybee quantity
Test method: will have 3 age the Americal rice leaf miner larva the Kidney bean seedling move in the dependent insect cage, totally 5 processing, and in 5 cages, insert respectively pea dive the female peak of fly Ji chalcid fly (accomplished mating, stalwartness and wait to lay eggs) be respectively 8 (connect the honeybee ratio! Feng ︰ fly) be 1:35; Queen bee is 8,3 age the Americal rice leaf miner larva be 280), 10 (connect the honeybee ratio! Feng ︰ fly) be 1: 30, queen bee is 10; 3 age the Americal rice leaf miner larva be 300), 13 (connect the honeybee ratio! Feng ︰ fly) be 1: 25; Queen bee is 13,3 age the Americal rice leaf miner larva be 325), 20 (connect the honeybee ratio! Feng ︰ fly) be 1: 20, queen bee is 20; 3 age the Americal rice leaf miner larva be 400), 40 (connect the honeybee ratio! Feng ︰ fly) be 1: 15; Queen bee is 40,3 age the Americal rice leaf miner larva be 600) head, in pin main body, lay eggs; To take over the Kidney bean blade that has pupa shape host larva of honeybee cuts, dries to host (inoculation 8d) when the pupa shape; And put into to go out the honeybee cage for a short time and cultivated 3-6 days, go out honeybee to the pea fly Ji chalcid fly of diving, write down out the honeybee number and produce female number.Every processing repeats for 5 times.Behind inoculation 24h, take out the Kidney bean seedling simultaneously, and under transmitted light, observe the death toll of Americal rice leaf miner larva.
Result of the test is following:
(A) difference connects the influence of honeybee comparison Americal rice leaf miner larva lethality rate
The pea fly Ji chalcid fly of diving connects the honeybee ratio to the average lethality rate of Americal rice leaf miner larva in difference! Feng ︰ fly) between have significant difference.
Formula=dead the number of average lethality rate/Americal rice leaf miner larva sum (%);
Connect honeybee and be respectively 60%, 70%, 92%, 95.0%, 90% than average lethality rate for 1:35,1:30,1: 25,1: 20,1: 15 o'clock Americal rice leaf miner larva;
The average lethality rate that connects the honeybee ratio and be 1: 25,1: 20,1: 15 o'clock Americal rice leaf miner larva is the highest, and all more than 89.8%, to connect honeybee more remarkable than difference with other.Wherein connect the honeybee ratio! Feng ︰ fly) be that 1: 20 o'clock beans fly Ji chalcid fly of diving reaches as high as 95.0% to the lethality rate of Americal rice leaf miner larva, and 1: 25 connect the honeybee ratio! Feng ︰ fly) very high, and cost is low, so this connects the peak than being suitable for most commercial production.
Dive the access quantity of fly Ji chalcid fly when 8 are increased to 13 (i.e. 1 ︰ 35-1 ︰ 25) when pea, and the average lethality rate of Americal rice leaf miner larva is ascendant trend linearly; (promptly 1: 25-1: in the time of 15), the variation of its average lethality rate is not obvious, only fluctuates at an equalization point to be increased to 40 from 13.(1: 20-1: in the time of 15), its average lethality rate does not only have corresponding raising to descend to some extent on the contrary and be increased to 40 from 20.
This explanation, connecing the honeybee ratio is 1: 25 o'clock, and pea is dived and just have been reached balance, saturated state between fly Ji chalcid fly and the host's Americal rice leaf miner, and continuing to increase the quantity that inserts the latent fly Ji chalcid fly of pea again can not play a positive role.
(B) difference connects the influence that the latent fly Ji chalcid fly offspring of honeybee comparison pea goes out honeybee number and eclosion rate
Difference connects dive fly Ji chalcid fly offspring's the honeybee number that goes out of honeybee comparison pea has appreciable impact, but between its eclosion rate there was no significant difference.
The formula of eclosion rate=emergence number/host larva pupa (%);
Connect honeybee and be respectively 80,88,90.2,90,85 than the honeybee number that goes out for 1:35,1:30,1: 25,1: 20,1: 15 o'clock Americal rice leaf miner larva;
Connect honeybee and be respectively 44%, 47%, 50.2%, 50.2%, 48% than eclosion rate for 1:35,1:30,1: 25,1: 20,1: 15 o'clock Americal rice leaf miner larva;
Connect honeybee than 1 ︰ 35-1 ︰ 25 between, along with the increase that connects honeybee quantity, what pea was dived fly Ji chalcid fly goes out the also corresponding increase with eclosion rate of honeybee number.When connecing the honeybee ratio and being 1 ︰ 25, it on average goes out the honeybee number and eclosion rate reaches peak, is respectively 90.2 and 50.2%.What pea was dived fly Ji chalcid fly on average goes out honeybee number and eclosion rate there was no significant difference when connecing the honeybee ratio and be 1 ︰ 25 and 1 ︰ 20.
Connect honeybee than when 1 ︰ 20 is increased to 1 ︰ 15, the dive honeybee number that on average goes out of fly Ji chalcid fly of pea does not only raise decline largely on the contrary with eclosion rate.This possibly be because pea is dived fly Ji chalcid fly to insert density excessive, vie each other in having caused planting, the generation of interference even hyperparasitism, make dive a big chunk ovum or the larva of fly Ji chalcid fly of pea can not accomplish its whole growth growth course.When connecing the honeybee ratio and being 1 ︰ 15, although higher slightly when gathering in the crops into the honeybee amount than 1 ︰, 35,1 ︰ 30, its eclosion rate is minimum.Therefore, this also side light in pea is dived the large-scale breeding process of fly Ji chalcid fly, be not to connect that honeybee is more, parasitic effects is good more, output capacity is high more.
(C) difference connects the influence of the latent female ratio of fly Ji chalcid fly offspring of honeybee comparison pea
Connect honeybee and be 1:1 than the female ratio of offspring for 1:35,1:30,1: 25,1: 20,1: 15 o'clock Americal rice leaf miner larva;
Difference connects the female ratio there was no significant difference of offspring of the latent fly Ji chalcid fly of honeybee comparison pea.That is to say that the pea fly Ji chalcid fly offspring queen bee number of diving is not connect the influence of honeybee quantity, and connect the honeybee ratio and do not have direct relation.Comprehensive above 3 small tests can know that it is comparatively suitable with 1: 25 to connect the honeybee ratio, save cost, improve space utilization efficient.Therefore by 1:25 the pea fly Ji chalcid fly of diving: the ratio of Americal rice leaf miner larva inserts the latent fly Ji chalcid fly of pea.
Can find out that from above-mentioned experiment the best-of-breed technology scheme that expands the latent fly Ji chalcid fly of numerous pea is following:
Be Kidney bean seedling+250 pair/m of 13d vegetative period
2Americal rice leaf miner adult+the connect honeybee of density than the fly Ji chalcid fly queen bee of diving for the pea of 1:25.
This scheme can be practiced thrift numerous honeybee cost greatly, can promote large-scale breeding and even the commercialization production of the latent fly Ji chalcid fly of pea.When the indoor propagation pea is dived fly Ji chalcid fly, dive fly Ji chalcid fly to becoming bee eclosion from plantation host plant Kidney bean, inoculation host Americal rice leaf miner, inoculation pea, need altogether (specifically to see table 3) about 40d.Dive fly Ji chalcid fly to gathering in the crops into honeybee from the inoculation pea, and whole numerous honeybee cycle is approximately 12-19d.In certain temperature range, the latent fly Ji chalcid fly of pea is along with rising shortening growth cycle of temperature, and therefore, the breeding cycle also can correspondingly shorten.
Table 3 pea is dived the fly Ji chalcid fly production cycle
Four, automatically collecting
The product collection technique can be divided into two types by the difference of collecting the worm attitude: one type is the collection pupa, and one type is to be gathered into honeybee, takes different collection methods respectively.
The method of collecting pupa is simpler, also is convenient to make the pupa card, and method is with the semiarid Kidney bean blade that has pupa; Insert in the tank that covers gauze, soaked 10-15 minute, during light shaking; The pupa of the latent fly Ji chalcid fly of pea can come off from blade, and floats on the upper strata, uses bamboo strainer to pull out and gets final product; After can waiting then to sprout wings, obtain the latent fly Ji chalcid fly of pea.
Pea capable of using is dived when collecting adult, and assemble the oriented top of the little bee imago of a fly Ji, the habit of the light that becomes, and the Kidney bean blade that will have the bee pupa of fast emergence is inserted in the small-sized cage, and cage is inverted funnel-form, encloses with lighttight black cloth, and opening is left in the top.Before collecting opening is tightened, inserted in the collection beehive, and in one of overhung incandescent lamp.After treating a large amount of adult eclosions, can open incandescent lamp, utilize the latent fly Ji chalcid fly of pea to become the phototaxis of honeybee, make it to be gathered in opening part, untie the opening of tightening in good time, the latent fly Ji chalcid fly of pea becomes honeybee to get into the collection beehive automatically.
Claims (10)
1. a method that expands the latent fly Ji chalcid fly of numerous pea comprises the steps:
The plant seedlings that 1) will have the latent fly Ji chalcid fly host larva of pea is put into foster worm container, inserts the latent fly Ji chalcid fly queen bee of pea to be laid eggs, and being cultured to said host's larvae development after in said host larva body, laying eggs is the pupa shape, obtains having the plant A of pupa;
2) take out the said plant A that has pupa and go out the honeybee cultivation, collect the latent fly Ji chalcid fly of pea, realize expanding numerous.
2. method according to claim 1 is characterized in that:
In the step 1), the latent fly Ji chalcid fly host of said pea is an Americal rice leaf miner,
The latent fly Ji chalcid fly host larva of said pea is the 2-3 Americal rice leaf miner in age in week.
3. method according to claim 1 and 2 is characterized in that:
In the step 1), the number ratio of latent fly Ji chalcid fly queen bee of said access pea and the latent fly Ji chalcid fly host larva of said pea is 1: (15-25).
4. according to arbitrary described method among the claim 1-3, it is characterized in that:
In the step 1), the number of latent fly Ji chalcid fly queen bee of said access pea and the latent fly Ji chalcid fly host larva of said pea is than being 1:25.
5. according to arbitrary described method among the claim 1-4, it is characterized in that:
In the step 1), said plant is a leguminous plant, and said leguminous plant is specially Kidney bean;
Said plant seedlings is specially 13-17 days Kidney bean seedling of growth; Said Kidney bean seedling is specially 13 days Kidney bean seedling of growth; 13 days Kidney bean seedling of said growth is that phaseolus vulgaris seeds is after planting cultivated the Kidney bean seedling that obtained in 13 days, does the 1st day from the planting seed note.
6. according to arbitrary described method among the claim 1-5, it is characterized in that:
Step 2) in, saidly goes out honeybee and cultivate to the blade of the said plant A that will take out and in going out the honeybee container, cultivated 3-6 days.
7. according to arbitrary described method among the claim 1-6, it is characterized in that:
Before step 1); Comprise that also preparation has the step of the plant seedlings of the latent fly Ji chalcid fly host larva of pea: in the foster worm container that is placed with 13 days Kidney bean seedling of said growth, insert paired Americal rice leaf miner; Lay eggs, cultivate, obtain having the plant seedlings of the latent fly Ji chalcid fly host larva of pea.
8. method according to claim 7 is characterized in that:
Said preparation has pea dives in the step of plant seedlings of fly Ji chalcid fly host larva, and said Americal rice leaf miner connects 250 pairs of Americal rice leaf miners according to every square metre of said foster worm container and inserts.
9. according to claim 7 or 8 described methods, it is characterized in that:
Said preparation has pea dives in the step of plant seedlings of fly Ji chalcid fly host larva, and said cultivation is cultivated 2 week-3 weeks of ovum for removing said Americal rice leaf miner at the said back 2h of laying eggs, from laying eggs day note work the 1st day.
10. according to arbitrary described method among the claim 1-9, it is characterized in that:
Step 2) in, the latent fly Ji chalcid fly of said collection pea is following A or B:
A, will in water, soak 10-15 minute, and shake simultaneously, collect said pupa, treat that said pupa sprouts wings, obtain the latent fly Ji chalcid fly of pea with the said pupa that comes off through the Kidney bean seedling leaves that has pupa that goes out after honeybee is cultivated;
B, the container that will place openend to seal through the Kidney bean seedling leaves that has pupa that goes out after honeybee is cultivated; And with said container place the collection beehive; Above said collection beehive, light is provided, after treating to dive said pea the little bee pupa of a fly Ji sprouting wings, opens the opening of said container; Utilize the latent fly Ji chalcid fly of said pea to become the phototaxis of honeybee, the latent fly Ji chalcid fly one-tenth of said pea honeybee gets into the completion of collection beehive automatically and collects.
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