CN1528135A - Process for industrial production for eretmocerus SP. - Google Patents
Process for industrial production for eretmocerus SP. Download PDFInfo
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- CN1528135A CN1528135A CNA031433146A CN03143314A CN1528135A CN 1528135 A CN1528135 A CN 1528135A CN A031433146 A CNA031433146 A CN A031433146A CN 03143314 A CN03143314 A CN 03143314A CN 1528135 A CN1528135 A CN 1528135A
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Abstract
The present invention provides a technological process for large-scale breeding bernisia tabaci genn by using tobacco and industrial production of eretmocerus spp. by using nymph of bernisia tabaci genn. Said invention adopts four-chamber eretmocerus spp. breeding method, mechanical collection, separation and purification, low-temp. short-period storage and annual industrial breeding eretmocerus spp. The host plant is tobacco leaf, it can bear lots of bernisia tabaci genn, and is high in production efficiency and is favorable for collection, the bernisia tabaci genn can be used as host of eretmocerus spp, so as to ensure controlling effect in field.
Description
Technical field
The present invention relates to a kind of useful insect (eretmocerus SP) batch production production and pest biological control technical field.
Background technology
At present, Bemisia tabaci has become worldwide major pest, and the plant species surplus in the of 600 that can cause harm is planted virus surplus propagating 70, protects especially in China's agricultural production that the ground vegetables have become that host range is the widest in producing, one of the most serious, insect that must keypoint control of causing harm.Because the Bemisia tabaci reproduction speed is fast, generation overlap, adult body surface be by characteristics such as waxs, adopts chemical pesticide control, not only bring pesticide resistance, insect problem such as wildness and residue of pesticide again, and preventive effect is very little.And be generally used for preventing and treating the problem of the parasite-Encarsia formosa of Trialeurodes vaporariorum Westwood owing to host's adaptability and environmental suitability, undesirable to the Bemisia tabaci control efficiency, eretmocerus SP has better control efficiency to Bemisia tabaci.In the prior art, domestic also do not have an eretmocerus SP batch production production technology, and the Encarsia formosa production technology is only arranged.This process using cucumber or tomato are raised Trialeurodes vaporariorum Westwood, produce Encarsia formosa with the aleyrodid nymph again, with the host's blade that the carries black pupa storage of drying in the shade.Exist the host plant blade little, carry the worm amount less, the daily management mission amount is big, production efficiency is low, be difficult for shortcoming such as batch production commercialization production.
Summary of the invention
The objective of the invention is to solve above deficiency, the technology that provides a kind of batch production to produce eretmocerus SP is bred Bemisia tabaci, is produced eretmocerus SP with the batch production of Bemisia tabaci nymph with tobacco, comprises following technical process:
(1) host plant is selected: select for use tobacco as the Bemisia tabaci host, have advantages such as blade hypertrophy, carrying aleyrodid amount is big, the blade face is smooth, be beneficial to and enhance productivity, be convenient to mechanization and collect.
Adopt four Room breeding bee methods: bee yield is many, grows neatly, and honeybee germplasm gauge is formatted.Between cleaning seedling workshop-aleyrodid seed receiving vehicle-aleyrodid nymphal development workshop-numerous honeybee workshop, wherein with aleyrodid nymphal development Workshop Production four age nymph return between the aleyrodid seed receiving vehicle.
(2) soil disinfection and fertilising: to disinfecting soil, soil can be recycled with liquor potassic permanganate; In soil, mix fertile ratio and mix fertilizer, be beneficial to and have a liking for the growth of nitrogen aleyrodid in the ratio of nitrogen, phosphorus, potassium 1: 0.2-0.5: 2.0-3.5.
(3) production of tobacco cleaning seedling: in seedling-cultivating tray, mix the soil sowing, transplant 2 times, be beneficial to the space and effectively utilize; Connect worm during to 8～10 leaves, top and the bottom leaf nutrition difference is little, is beneficial to insect and grows neat.This workshop requires clean environment, airtight, so that cultivate the tobacco cleaning seedling (without pesticide-treated) of anosis worm; Temperature remains on 22～28 ℃, and relative moisture remains on 70～85%, the illumination abundance.
(4) inoculation aleyrodid: will wait to connect the cleaning seedling and move into and connect the worm workshop, connect 6000～8000/strain of Bemisia tabaci adults, average every square centimeter of 4 adults, the density of laying eggs reaches 20～30 every square centimeter.Inoculate after 24 hours, drive adult whitefly away, move aleyrodid nymphal development workshop to.Temperature remains on about 26 ℃ between seed receiving vehicle, relative moisture about 70%, illumination abundance.
(5) growth of aleyrodid nymph: postvaccinal plant is moved into and grows the workshop, strengthens rich water quality management, and left and right sides ovum hatching in 6 days is nymph, and 15-17 days nymphal development to 2～3 ages promptly can be used for inoculating eretmocerus SP this moment.The environmental condition of growing the workshop is with cleaning seedling workshop.
(6) inoculate eretmocerus SP: the plant in aleyrodid nymphal development to 2～3 ages is moved to connect the honeybee workshop.Every strain connects about 3000 of the eretmocerus SPs of firm emergence, and average 1～2 every square centimeter becomes honeybee.Promptly there was the parasitic pupa of brown more than 90% to occur through about 14 days.Between this workshop temperature, humidity, illumination are with the aleyrodid seed receiving vehicle.Different was to need light filling sooner or later, with satisfied 12 hours light application time.
(7) eretmocerus SP is collected and is separated: will have the plant leaf of the parasitic pupa of brown to take, and wash away with pressure (hydraulic) water, and remove not by the yellow aleyrodid pupa of parasitism with separator, and keep the brown pupa.
(8) eretmocerus SP purifying: adopt the impurity elimination of aphid chalcid fly purifier, purification, can store or do card then.
(9) the eretmocerus SP pupa is stored: deposit parasitic bee pupa with 9 ± 0.5 ℃ of insulating boxs, can do 1 month deposit.
Effect of the present invention is: with the host of tobacco as Bemisia tabaci, and the blade hypertrophy, smooth being convenient in blade face collected, and loaded area is big, and every strain can be received 2～30,000, and crop cycle is short, the space availability ratio height; Adopt Bemisia tabaci to do the host of parasitic wasp, utilized natural host, help keeping the parasitic capacity of eretmocerus SP, guarantee field control effect, be better than utilizing vector breeding natural enemy, saved this operation of natural enemy insect kind rejuvenation Bemisia tabaci; Adopt mechanization to collect and separate the eretmocerus SP pupa, its eclosion rate does not reduce, and is easy to commercialization, and stores, transports, uses all very convenient; Adopt four Room breeding bee method bee yields many, grow neatly, go out the honeybee time to concentrate, honeybee germplasm gauge is formatted, and is convenient to planned production; The realization anniversary numerous honeybee and short, the supply and demand that has solved biological control is problem non-timely.
Embodiment
Embodiment 1:
To produce 100,000 eretmocerus SPs is that example further specifies:
(1) preparation greenhouse: the production of cleaning seedling needs 2.5 square metres, and the aleyrodid inoculation needs 2 square metres, and the aleyrodid nymphal development needs 2 square metres, and numerous honeybee needs 1.5 square metres, amounts to 8 square metres; Each workshop isolates mutually, avoids insect to scurry and flies to infect.
(2) cleaning seedling production: in seedling-cultivating tray, mix soil and sow 10 tobacco seeds, 1 in every cave; Long transplant (moving in the little basin) to 5cm for the first time when high, transplant (moving in the big basin) when 15cm is high for the second time, the long preparation of pinching off young shoots when 10 leaves are arranged connects worm.This workshop requires clean environment, airtight, so that cultivate the tobacco cleaning seedling (without pesticide-treated) of anosis worm; Temperature remains on 25 ℃, and relative moisture remains on 80%, the illumination abundance.
(3) aleyrodid inoculation: remove 8 strains cleaning seedling and enter between the aleyrodid seed receiving vehicle, every strain connects 7000, promptly average every square centimeter of 4 adults, 30/square centimeter of the density of laying eggs; Connect foot after 24 hours, drive adult away.Temperature remains on 25 ℃ between seed receiving vehicle, relative moisture 80%, illumination abundance.
(4) aleyrodid nymphal development: aleyrodid nymphal development workshop is moved in 8 strains that will inoculate, strengthen rich water quality management, the aleyrodid nymphal development is to 3 ages during 16 days left and right sides, remove 5 strains to connecing the honeybee workshop, return between the aleyrodid seed receiving vehicle when remaining 3 strains are grown to 4 ages, allow it sprout wings and keep the stable of adult population between the aleyrodid seed receiving vehicle to replenish.The environmental condition of growing the workshop is with cleaning seedling workshop.
(5) numerous honeybee: after 5 strains of waiting to connect honeybee entered numerous honeybee workshop, every strain connect 3000 of the eretmocerus SPs of firm emergence, guarantees to have on every square centimeter of blade 2 to become honeybees.When 14 days left and right sides, the aleyrodid pupa overstrike more than 90% is parasitic bee pupa.This workshop moisture temperature, illumination are with between the aleyrodid seed receiving vehicle; Different was to need light filling sooner or later, with satisfied 12 hours light application time.
(6) parasitic wasp collection, separation, purifying: take blade, collect, and remove not by the yellow pupa of parasitism, keep the brown pupa, again with storing or do card behind the aphid chalcid fly purifier purifying with separator with scour without sediment motion.
Claims (2)
1, eretmocerus SP technology is produced in a kind of batch production, uses tobacco large-scale breeding Bemisia tabaci, produces eretmocerus SP with the batch production of Bemisia tabaci nymph, and its technology comprises following process:
(1) host plant is selected: select for use tobacco as the Bemisia tabaci host;
(2) soil disinfection and fertilising: to disinfecting soil, in soil, mix fertilizer with liquor potassic permanganate, be beneficial to and have a liking for the growth of nitrogen aleyrodid;
(3) production of tobacco cleaning seedling: mix the soil sowing in seedling-cultivating tray, transplant 2 times, connect worm during to 8～10 leaves, temperature remains on 22～28 ℃, and relative moisture remains on 70～85%, the illumination abundance.
(4) inoculation aleyrodid: will wait to connect the cleaning seedling and move into and connect the worm workshop, connect 6000～8000/strain of Bemisia tabaci adults, average every square centimeter of 4 adults, the density of laying eggs reaches 20～30 every square centimeter, inoculates after 24 hours, drives adult whitefly away, move aleyrodid nymphal development workshop to, temperature remains on about 26 ℃ between seed receiving vehicle, relative moisture about 70%, illumination abundance;
(5) growth of aleyrodid nymph: postvaccinal plant is moved into and grows the workshop, strengthen rich water quality management, left and right sides ovum hatching in 6 days is nymph, 15-17 days nymphal development to 2～3 ages, promptly can be used for inoculating eretmocerus SP this moment, the environmental condition of growing the workshop is with cleaning seedling workshop;
(6) inoculate eretmocerus SP: the plant in aleyrodid nymphal development to 2～3 ages is moved to connect the honeybee workshop.Every strain connects about 3000 of the eretmocerus SPs of firm emergence, and average 1～2 every square centimeter becomes honeybee.Promptly there was the parasitic pupa of brown more than 90% to occur through about 14 days.Between this workshop temperature, humidity, illumination are with the aleyrodid seed receiving vehicle.Different was to need light filling sooner or later, with satisfied 12 hours light application time;
(7) eretmocerus SP is collected and is separated: will have the plant leaf of the parasitic pupa of brown to take, and wash away with pressure (hydraulic) water, and remove not by the yellow aleyrodid pupa of parasitism with separator, and keep the brown pupa;
(8) eretmocerus SP purifying: adopt the impurity elimination of aphid chalcid fly purifier, purification, can store or do card then;
(9) the eretmocerus SP pupa is stored: deposit parasitic bee pupa with 9 ± 0.5 ℃ of insulating boxs, can do 1 month deposit.
2, eretmocerus SP technology is produced in batch production according to claim 1, it is characterized in that mixing fertile ratio in the soil by nitrogen, phosphorus, potassium 1: 0.2-0.5: 2.0-3.5 in described soil disinfection and fertilising operation.
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| CN101390505B (en) * | 2008-10-28 | 2012-02-15 | 北京市农林科学院 | Encarsia Sophia artificial propagation production method |
| CN102630647A (en) * | 2012-05-03 | 2012-08-15 | 中国农业科学院植物保护研究所 | Method for propagating Diglyphus isaea |
| CN102771446A (en) * | 2012-08-07 | 2012-11-14 | 中国农业科学院植物保护研究所 | Propagation method of Diglyphus isaea by use of Chromatomyia horticola |
| CN102870742A (en) * | 2012-09-24 | 2013-01-16 | 中国农业科学院植物保护研究所 | Large-scale artificial feeding method for eretmocerus hayati |
| CN103749400A (en) * | 2014-01-22 | 2014-04-30 | 云南省烟草公司玉溪市公司 | Method for improving breeding efficiency of bemisia tabaci |
| CN104642267A (en) * | 2015-02-09 | 2015-05-27 | 云南绿叶生防科技有限公司 | Industrial cultivation method for aphidius gifuensis ashmead |
| CN105052625A (en) * | 2015-07-20 | 2015-11-18 | 胡永军 | Method for preventing and treating facility vegetable bemisia tabaci through eretmocerus sp |
| CN105165737A (en) * | 2015-10-13 | 2015-12-23 | 中国农业科学院棉花研究所 | Artificial feeding method of cotton aphid parasitoid wasp-Lysiphlebia japonica |
| CN105900932A (en) * | 2016-05-10 | 2016-08-31 | 云南省烟草公司玉溪市公司 | Method for efficiently breeding Lipaphis erysimi |
| CN111758673A (en) * | 2020-07-16 | 2020-10-13 | 中国热带农业科学院环境与植物保护研究所 | Method for expanding propagation of spodoptera frugiperda trichogramma by preserving spodoptera frugiperda egg masses |
| CN113508783A (en) * | 2021-04-28 | 2021-10-19 | 天津市农业科学院 | Method for breeding large quantities of Aphidius aurantiaca by using cotton seedlings and MED bemisia tabaci |
| CN115868459A (en) * | 2022-12-16 | 2023-03-31 | 福建省农业科学院水稻研究所 | Application of manila as spawning carrier of small broad-head planthopper in cold storage of small rice-lice tassel bees and cold storage method of small rice-lice tassel bees |
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- 2003-09-28 CN CN 03143314 patent/CN1211010C/en not_active Expired - Fee Related
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101390505B (en) * | 2008-10-28 | 2012-02-15 | 北京市农林科学院 | Encarsia Sophia artificial propagation production method |
| CN102630647A (en) * | 2012-05-03 | 2012-08-15 | 中国农业科学院植物保护研究所 | Method for propagating Diglyphus isaea |
| CN102630647B (en) * | 2012-05-03 | 2014-05-07 | 中国农业科学院植物保护研究所 | Method for propagating Diglyphus isaea |
| CN102771446A (en) * | 2012-08-07 | 2012-11-14 | 中国农业科学院植物保护研究所 | Propagation method of Diglyphus isaea by use of Chromatomyia horticola |
| CN102771446B (en) * | 2012-08-07 | 2014-06-04 | 中国农业科学院植物保护研究所 | Propagation method of Diglyphus isaea by use of Chromatomyia horticola |
| CN102870742A (en) * | 2012-09-24 | 2013-01-16 | 中国农业科学院植物保护研究所 | Large-scale artificial feeding method for eretmocerus hayati |
| CN103749400A (en) * | 2014-01-22 | 2014-04-30 | 云南省烟草公司玉溪市公司 | Method for improving breeding efficiency of bemisia tabaci |
| CN103749400B (en) * | 2014-01-22 | 2015-05-06 | 云南省烟草公司玉溪市公司 | Method for improving breeding efficiency of bemisia tabaci |
| CN104642267A (en) * | 2015-02-09 | 2015-05-27 | 云南绿叶生防科技有限公司 | Industrial cultivation method for aphidius gifuensis ashmead |
| CN104642267B (en) * | 2015-02-09 | 2018-05-04 | 云南绿叶生防科技有限公司 | A kind of industrialization breeding method of aphidius gifuensis |
| CN105052625A (en) * | 2015-07-20 | 2015-11-18 | 胡永军 | Method for preventing and treating facility vegetable bemisia tabaci through eretmocerus sp |
| CN105165737A (en) * | 2015-10-13 | 2015-12-23 | 中国农业科学院棉花研究所 | Artificial feeding method of cotton aphid parasitoid wasp-Lysiphlebia japonica |
| CN105165737B (en) * | 2015-10-13 | 2017-10-31 | 中国农业科学院棉花研究所 | A kind of rearing method of cotten aphid parasitic wasp cotton aphid parasite |
| CN105900932A (en) * | 2016-05-10 | 2016-08-31 | 云南省烟草公司玉溪市公司 | Method for efficiently breeding Lipaphis erysimi |
| CN105900932B (en) * | 2016-05-10 | 2019-09-10 | 云南省烟草公司玉溪市公司 | A kind of method of efficient breeding radish aphid |
| CN111758673A (en) * | 2020-07-16 | 2020-10-13 | 中国热带农业科学院环境与植物保护研究所 | Method for expanding propagation of spodoptera frugiperda trichogramma by preserving spodoptera frugiperda egg masses |
| CN111758673B (en) * | 2020-07-16 | 2021-11-23 | 中国热带农业科学院环境与植物保护研究所 | Method for expanding propagation of spodoptera frugiperda trichogramma by preserving spodoptera frugiperda egg masses |
| CN113508783A (en) * | 2021-04-28 | 2021-10-19 | 天津市农业科学院 | Method for breeding large quantities of Aphidius aurantiaca by using cotton seedlings and MED bemisia tabaci |
| CN115868459A (en) * | 2022-12-16 | 2023-03-31 | 福建省农业科学院水稻研究所 | Application of manila as spawning carrier of small broad-head planthopper in cold storage of small rice-lice tassel bees and cold storage method of small rice-lice tassel bees |
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Application publication date: 20040915 Assignee: Hengshui bee Biotechnology Co.,Ltd. Assignor: Dry-Land Farming Institute of Hebei Academy of Agricultural and Forestry Sciences Contract record no.: 2015130000117 Denomination of invention: Process for industrial production for eretmocerus SP. Granted publication date: 20050720 License type: Exclusive License Record date: 20150730 |
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