CN102273432A - Method for producing drone of light yellow encarsia amicula by taking encarsia formosa gahan as breeding host - Google Patents
Method for producing drone of light yellow encarsia amicula by taking encarsia formosa gahan as breeding host Download PDFInfo
- Publication number
- CN102273432A CN102273432A CN2011101733449A CN201110173344A CN102273432A CN 102273432 A CN102273432 A CN 102273432A CN 2011101733449 A CN2011101733449 A CN 2011101733449A CN 201110173344 A CN201110173344 A CN 201110173344A CN 102273432 A CN102273432 A CN 102273432A
- Authority
- CN
- China
- Prior art keywords
- pale yellow
- drone
- host
- chalcid fly
- honeybee
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention discloses a method for producing a drone of light yellow encarsia amicula by taking encarsia formosa gahan as a breeding host. The light yellow encarsia amicula is a potential ideal natural enemy for controlling international insect pest bemisa babaci. Under natural conditions, the light yellow encarsia amicula are few, and capability of controlling the bemisa babaci is restricted to the utmost extent. Production and release of the drone are important for the light yellow encarsia amicula to control the bemisa babaci. In the invention, unmated drone of the light yellow encarsia amicula is released, the encarsia Formosa in a three age-prepupa stage is taken as a secondary host for breeding the drone, and high-quality and high-efficiency production of the light yellow encarsia amicula is realized. The drone of the light yellow encarsia amicula produced by adopting the invention has the advantages such as short development duration, high emergence rate, large body and obvious increase of parasitic quantity after the drone is mated with a queen bee and the like and can be effectively used for biological control of mealywing insect pests such as the bemisa babaci and the like.
Description
Technical field
The present invention relates to rapidly and efficiently breed the method for pale yellow grace aphid chalcid fly drone, further provided a kind of Encarsia formosa of using, belong to pest biological control technical field as the method that the breeding host produces pale yellow grace aphid chalcid fly drone.
Background technology
Bemisia tabaci has become the international difficult problem in the agricultural production.This insect status rise main with Type B Bemisia tabaci in recent years
Bemisia tabaciThe successful invasion of (claiming Bemisia argentifolii again) is relevant.Kind surplus the host plant that Bemisia tabaci causes harm surpasses 600 is got the growing way that not only slackens crop after food is caused harm, and can also cause the irregular maturation of the physiological metabolism imbalance of plant as reaction of custard squash silver leaf and tomato.In addition, can also cause more serious causing harm indirectly, the honeydew of secretion not only influences photosynthesis of plants, can also grow the growth of mould, thereby reduces the price and the quality of agricultural product.The more important thing is, can cause being very popular of viral diseases of plants as the insect-borne transmission virus disease.Present this insect is distributed widely in all over the world, has become one of most important insect in land for growing field crops broad leaf crop, vegetables and gardening, the gardens production.
Applying pesticides is still the most important method of control aleyrodid class pest at present.Chemical pesticide especially wide spectrum high-toxic pesticide not only directly threatens human beings'health, goes back serious environment pollution.Along with a large amount of uses of agricultural chemicals, also cause the continuous rising of pest resistance to insecticide and seriously slackening of natural enemy nature control action.In the long run, the biological control of application natural enemy insect is the inexorable trend of administering these insects.Particularly the biological control of carrying out " controlling worm with worm " for the higher vegetables of some economic worths and horticultural crop becomes real more feasible.
Parasitic wasp can kill the host by two kinds of approach, and the one, parasitic, ovum originated in the pin main body or external, finally kill them by larval feeding; Another is to get the food host, thereby female one-tenth honeybee kills them by getting food host's body fluid and hemolymph.Utilize grace aphid chalcid fly belong to (
Encarsia) and blade angle aphid chalcid fly genus (
Eretmocerus) many trials of parasitic wasp control aleyrodid have all obtained certain success.From present application present situation, Encarsia formosa
E. formosaBe that to commercially produce degree the highest, and control aleyrodid class pest is used the most general parasitic wasp under greenhouse experiment, this parasitic wasp is considered to control Trialeurodes vaporariorum Westwood
Trialeurodes vaporariorumThe most desirable natural enemy, but this parasitic wasp can't be controlled the generation of Bemisia tabaci effectively and cause harm.Pale yellow grace aphid chalcid fly
E. sophiaIt is a kind of facultative super parasitic wasp, different with the modes of reproduction of traditional parasitic wasp, the queen bee of mating can originate in fertilized egg in the aleyrodid nymph body, grows future to be female worm, and the queen bee of mating then produces male worm in the future by parasitic female descendant of oneself planting interior or allied species.Result of study shows, parasitic ability and the Encarsia formosa of the pale yellow grace aphid chalcid fly of normal mating are suitable, but the female one-tenth honeybee of this parasitic wasp to get food host's ability be three times of the latter.In general, the effect of pale yellow grace aphid chalcid fly control Bemisia tabaci is a potential desirable natural enemy of preventing and treating international insect Bemisia tabaci at present significantly better than Encarsia formosa.But, under field conditions (factors), the quantity rareness of pale yellow grace aphid chalcid fly drone, the ability of their control Bemisia tabaci is greatly limited.Therefore, production of drone and release are most important for the effect of the pale yellow grace aphid chalcid fly control of performance aleyrodid.
Summary of the invention
The invention provides a kind of Encarsia formosa of using and produce the method for pale yellow grace aphid chalcid fly drone, be a kind of new technology of breeding pale yellow grace aphid chalcid fly drone, can be and scientific and effectively utilize pale yellow grace aphid chalcid fly to administer the Bemisia tabaci insect to establish good basis as the breeding host.
The technical solution that the present invention adopts may further comprise the steps:
(1) cultivation of host plant
Sow, grow seedlings and the field planting host plant according to conventional method; Cultivate host plant in the controlled greenhouse of illumination abundance, environmental condition, when seedling growth during to 3 true leaves of tool, individual plant is colonizated in the flowerpot; It is standby when host plant grows to 8-10 sheet true leaf;
(2) elementary host's inoculation is bred
The host plant of getting ready is put between the aleyrodid breeding, and the host plant of aleyrodid is raised in shake, adult whitefly is diffused on the plant of new access, behind the 24h, receive ovum and finish, and change the host plant of tool aleyrodid ovum over to insect and grow the chamber (it is 26-28 ℃ that insect grows the room environmental condition, 60-70% R.H., 14:10 L:D, down together), behind the 12d, when aleyrodid nymphal development to four standby during the initial stage in age;
(3) secondary or secondary host's breeds
The host plant of tool aleyrodid nymph in four ages is moved into numerous honeybee chamber, and (environmental condition is 26-28 ℃, 60-70% R.H., 14:10 L:D), insert secondary host honeybee and become honeybee (connect honeybee quantity with honeybee: nymph 〉=1:30 is advisable), remove secondary host honeybee behind the 48h and become honeybee, and change host plant over to insect growth chamber; Behind the 7d, check the development progress of secondary host honeybee, when parasitic wasp grows to third-instar larvae-prepupa standby during the stage;
(4) female preparation for pale yellow grace aphid chalcid fly
The host plant of tool aleyrodid nymph in four ages is moved into numerous honeybee chamber, insert the pale yellow grace aphid chalcid fly (female, drone is pressed 3:1 and mixes access) of mating, go honeybee (when going honeybee behind the 48h, host plant is moved into confined chamber, pharmacy fumigation 3h, kill the parasitic wasp of leaving over), and change host plant over to insect and grow the chamber; Behind the 13d, the parasitic wasp of emergence all is pale yellow grace aphid chalcid fly virgin honeybee, these not the queen bee of mating will be directly used in the drone that parasitic secondary host produces pale yellow grace aphid chalcid fly;
(5) pale yellow grace aphid chalcid fly drone breeds
The of the right age secondary host's of above-mentioned (3) step tool (tritonymph-prepupa stage Encarsia formosa) host plant is moved into numerous honeybee chamber, to just sprout wings simultaneously (＜24 h) not the pale yellow grace aphid chalcid fly queen bee of mating insert, connecing honeybee quantity is pale yellow grace aphid chalcid fly: secondary host 〉=1:30, remove pale yellow grace aphid chalcid fly behind the 72h; Behind the 9d, the development progress of the pale yellow grace aphid of microscopy chalcid fly drone, when black pupa rate is higher than 95%, the blade of plucking a plant, making honeybee card, packing storage or direct and queen bee field release application.
Pale yellow grace aphid chalcid fly drone mating system of the present invention is characterized in that: the described secondary or secondary host of step (3) breeds, and the secondary host who breeds is an Encarsia formosa
E. formosa
Pale yellow grace aphid chalcid fly drone mating system of the present invention is characterized in that: the described secondary or secondary host of step (3) breeds, for the parasitic developmental stage of producing the Encarsia formosa of drone of pale yellow grace aphid chalcid fly be third-instar larvae-prepupa.
Pale yellow grace aphid chalcid fly drone mating system of the present invention is characterized in that: the preparation of step (4) pale yellow grace aphid chalcid fly of described female generation, being used for the mother that parasitic secondary host produces drone is the pale yellow grace aphid chalcid fly queen bee of not mating all in generation.
Pale yellow grace aphid chalcid fly drone mating system of the present invention is characterized in that: the breeding of the described pale yellow grace aphid chalcid fly drone of step (5), connecing honeybee quantity is pale yellow grace aphid chalcid fly queen bee: secondary host 〉=1:30 connects the honeybee time for being not less than 72h.
Good effect of the present invention is: Bemisia tabaci is a kind of international important pests, at present also not efficiently parasitic enemy insect can control it and cause harm.Comprehensive parasitism and the prevention and control effect of getting the food host, pale yellow grace aphid chalcid fly is considered to control the more satisfactory parasitic wasp of Bemisia tabaci, but the burst size of drone is to the performance decisive role of its effect.The present invention is a kind of new technology of breeding pale yellow grace aphid chalcid fly drone, can be scientific and effectively to utilize pale yellow grace aphid chalcid fly to administer the Bemisia tabaci insect to establish good basis.
Description of drawings
Fig. 1 produces the main flow chart of breeding for the present invention.
Embodiment
By following examples the present invention is described for example further, and do not limit the present invention in any way, under the prerequisite that does not deviate from technical solution of the present invention, any change or change that those of ordinary skills that the present invention did are realized easily all will fall within the claim scope of the present invention.
Embodiment 1
With the Bemisia tabaci is the pale yellow grace aphid chalcid fly drones of a large amount of breedings of host insect
Pale yellow grace aphid chalcid fly drone produces the main process of breeding as shown in Figure 1 in a large number, and the concrete operations of using 10000 drones of this invention production are as follows:
1) cultivation of host plant
Sow, grow seedlings and the field planting tomato according to conventional method.Cultivate tomato plant in the controlled greenhouse of illumination abundance, environmental condition, when seedling growth during to 3 true leaves of tool, individual plant is colonizated in (bore 18cm, dark 12cm) in the flowerpot, needs 10 basins altogether.When tomato growth during to 8-10 sheet true leaf, remove lobus cardiacus (help to keep the extension growth of blade, and can prevent plant excessive growth), standby when keeping leaf growth to about 8cm length.
2)
Elementary host's inoculation is bred
The tomato plant of getting ready is put between the Bemisia tabaci breeding, and the host plant of aleyrodid is raised in shake, adult whitefly is diffused on the plant of new access, behind the 24h, the ovum number of sampling observation blade back, when the average ovum amount of blade back is higher than 200, the receipts ovum finishes, and with the tomato plants of tool aleyrodid ovum change over to insect grow the chamber (26-28 ℃, 60-70% R.H., 14:10 L:D, down together).Behind the 12d, when aleyrodid nymphal development to four standby during the initial stage in age.
3) secondary (or secondary) host's breeds
The tomato plant of tool aleyrodid nymph in four ages is moved into numerous honeybee chamber (condition is grown the chamber with insect), insert Encarsia formosa and become 500 of honeybees, remove Encarsia formosa behind the 48h and become honeybee, and change tomato plant over to insect growth chamber.Behind the 7d, check the development progress of Encarsia formosa, when parasitic wasp grows to third-instar larvae-prepupa standby during the stage.
4) female preparation for pale yellow grace aphid chalcid fly
The tomato plant of getting 1 basin tool aleyrodid nymph in four ages moves into numerous honeybee chamber, and 50 of the pale yellow grace aphid chalcid flies of access mating go honeybee (when going honeybee behind the 48h, host plant is moved into confined chamber, pharmacy fumigation 3h kills the parasitic wasp of leaving over), and change tomato plant over to insect and grow the chamber.Behind the 13d, about 1000 parasitic wasps of emergence all are pale yellow grace aphid chalcid fly virgin honeybee, these not the queen bee of mating will be directly used in the drone that parasitic secondary host produces pale yellow grace aphid chalcid fly.
5) pale yellow grace aphid chalcid fly drone breeds
The of the right age secondary host's of above-mentioned (3) step tool (tritonymph-prepupa stage Encarsia formosa) tomato plant is moved into numerous honeybee chamber, the pale yellow grace aphid chalcid fly virgin honeybee that above-mentioned (4) step is sprouted wings is inserted simultaneously, connect honeybee quantity and be not less than 500, remove pale yellow grace aphid chalcid fly behind the 72h.Behind the 9d, the development progress of the pale yellow grace aphid of microscopy chalcid fly drone, when black pupa rate is higher than 95%, the blade of plucking a plant, making honeybee card, packing storage or direct and together field release application of queen bee.Detect through experiment, the eclosion rate that utilizes Encarsia formosa to breed pale yellow grace aphid chalcid fly drone is higher than 80%.
Embodiment 2
Pale yellow grace aphid chalcid fly is to the secondary host's of difference selection
1 materials and methods
1.1 for the examination insect
Host insect: Type B Bemisia tabaci
Bemisia tabaci(Gennadius)
Parasitic wasp: pale yellow grace aphid chalcid fly
Encarsia sophia(Girault ﹠amp; Dodd); Encarsia formosa
Encarsia formosaGahan; Black dull blade angle aphid chalcid fly
Eretmocerus melanoscutusZolnerowich ﹠amp; Rose.
For the examination host plant
Wild cabbage
Brassica oleraceaL. var.
CapitataIt is standby when potted plant wild cabbage grows to three true leaves.
Test method
1.3.1 inferior level host selects test
When for examination secondary host pale yellow grace aphid chalcid fly, Encarsia formosa and black dull blade angle aphid chalcid fly queen bee larvae development to three age grade section, cabbage leaves cut and with absorbent cotton parcel petiole water planting in the measuring cup of 60ml.Microscopy is removed not parasitic aleyrodid nymph and is not reached the parasitic wasp individuality of developmental stage, makes each blade keep 20 parasitic wasp larva individualities of thirsting for the stage, and puts it into transparent connecing in the honeybee box.Sprout wings at the beginning of introducing 2 the pale yellow grace aphid chalcid fly queen bee of not mating goes honeybee behind the 48h, each secondary host of microscopy is by the quantity of parasitism behind the 6d.Behind the 10d, write down each secondary host every day and go up the drone quantity of sprouting wings, and measure the size (measuring the long and head capsule width of body) of drone, finally add up development duration and eclosion rate.Each is handled and repeats 25 times.
The originate functional examination of drone of different secondary hosts
Get the pale yellow grace aphid chalcid fly queen bee of the not mating of sprouting wings at the beginning of 1, respectively with the not drone pairing of mating of the first emergence of 1 separate sources, and with its insert blade have about 60 three age the aleyrodid nymph little worm cage in, every 24h, change it over to little worm cage with similar amt aleyrodid nymph, until queen bee death, finally add up total parasitic quantity.Each is handled and repeats 20 times.
Above-mentioned test is all carried out (26-28 ° of C, 60-70% R.H. and 14:10 L:D) in that the artificial climate of standard is indoor.
Result and analysis
Result of study shows that pale yellow grace aphid chalcid fly queen bee more preference utilizes Encarsia formosa as producing male secondary host.When female larva of bee was as the male host of product in Encarsia formosa, black dull blade angle aphid chalcid fly and this kind are provided, parasitic percentage was respectively 76.2%, 45.6% and 26.6%.With Encarsia formosa when producing male host, pale yellow grace aphid chalcid fly drone is faster developmentally, and has higher eclosion rate (84%), it is bigger that the emergence drone obviously goes up the emergence individuality than other host.The more important thing is, compare with other source drone, when pale yellow grace aphid chalcid fly queen bee and the drone post-coitum of breeding with Encarsia formosa, can parasitic more Bemisia tabaci nymph (78.5 vs 52.8-55.0).
In general, Encarsia formosa is to breed the optimal secondary host of pale yellow grace aphid chalcid fly drone.
Claims (5)
1. pale yellow grace aphid chalcid fly drone mating system may further comprise the steps:
1) cultivation of host plant
Sow, grow seedlings and the field planting host plant according to conventional method; In the controlled greenhouse of illumination abundance, environmental condition, cultivate host plant, standby when host plant grows to 8-10 sheet true leaf;
2)
Elementary host's inoculation is bred
The host plant of getting ready is put into aleyrodid breeds the chamber, behind the 24h, receive ovum and finish, and change the host plant of tool aleyrodid ovum over to insect and grow the chamber, behind the 12d, when aleyrodid nymphal development to four standby during the initial stage in age;
3) secondary or secondary host's breeds
The host plant of tool aleyrodid nymph in four ages is moved into numerous honeybee chamber, insert secondary host honeybee, remove the one-tenth honeybee of secondary host honeybee behind the 48h, and change host plant over to insect and grow the chamber, behind the 7d, check the development progress of secondary host honeybee, when parasitic wasp grows to third-instar larvae-prepupa standby during the stage;
4) female preparation for pale yellow grace aphid chalcid fly
The host plant of tool aleyrodid nymph in four ages is moved into numerous honeybee chamber, insert the pale yellow grace aphid chalcid fly queen bee of mating, go honeybee behind the 48h, and change host plant over to insect and grow the chamber, behind the 13d, the parasitic wasp that sprouts wings all is the pale yellow grace aphid chalcid fly virgin honeybee of not mating, these not the queen bee of mating will be directly used in the drone that parasitic secondary host produces pale yellow grace aphid chalcid fly;
5) pale yellow grace aphid chalcid fly drone breeds
Above-mentioned (3) the of the right age secondary hosts' of step tool (tritonymph-prepupa) host plant is moved into numerous honeybee chamber, (＜24 h) pale yellow grace aphid chalcid fly virgin honeybee that will just sprout wings is simultaneously inserted, connecing honeybee quantity is pale yellow grace aphid chalcid fly: secondary host 〉=1:30, remove pale yellow grace aphid chalcid fly behind the 72h, behind the 9d, the development progress of the pale yellow grace aphid of microscopy chalcid fly drone, when black pupa rate is higher than 95%, the blade of plucking a plant is made the honeybee card, packing storage or directly discharge application with the queen bee field.
2. pale yellow according to claim 1 grace aphid chalcid fly drone mating system is characterized in that: the described secondary or secondary host of step (3) breeds, and the secondary host who breeds is an Encarsia formosa
Encarsia formosa
3. pale yellow according to claim 1 grace aphid chalcid fly drone mating system is characterized in that: the described secondary or secondary host of step (3) breeds, for the parasitic developmental stage of producing the Encarsia formosa of drone of pale yellow grace aphid chalcid fly be third-instar larvae-prepupa.
4. pale yellow grace aphid chalcid fly drone mating system as claimed in claim 1 is characterized in that: the preparation of step (4) pale yellow grace aphid chalcid fly of described female generation, being used for the mother that parasitic secondary host produces drone is the pale yellow grace aphid chalcid fly queen bee of not mating all in generation.
5. pale yellow grace aphid chalcid fly drone mating system as claimed in claim 1 is characterized in that: the breeding of the described pale yellow grace aphid chalcid fly drone of step (5), connecing honeybee quantity is pale yellow grace aphid chalcid fly queen bee: secondary host 〉=1:30 connects the honeybee time for being not less than 72h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110173344 CN102273432B (en) | 2011-06-26 | 2011-06-26 | Method for producing drone of light yellow encarsia amicula by taking encarsia formosa gahan as breeding host |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110173344 CN102273432B (en) | 2011-06-26 | 2011-06-26 | Method for producing drone of light yellow encarsia amicula by taking encarsia formosa gahan as breeding host |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102273432A true CN102273432A (en) | 2011-12-14 |
| CN102273432B CN102273432B (en) | 2012-12-26 |
Family
ID=45099425
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110173344 Expired - Fee Related CN102273432B (en) | 2011-06-26 | 2011-06-26 | Method for producing drone of light yellow encarsia amicula by taking encarsia formosa gahan as breeding host |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102273432B (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102630647A (en) * | 2012-05-03 | 2012-08-15 | 中国农业科学院植物保护研究所 | Method for propagating Diglyphus isaea |
| CN102870742A (en) * | 2012-09-24 | 2013-01-16 | 中国农业科学院植物保护研究所 | Large-scale artificial feeding method for eretmocerus hayati |
| CN103283540A (en) * | 2013-06-26 | 2013-09-11 | 广西壮族自治区林业科学研究院 | Method for biological control over mangrove plants primary pest Aegiceras Chaetognatha tortricidae small leaf rollers |
| CN103385220A (en) * | 2013-07-25 | 2013-11-13 | 任伟 | Scale breeding method for encarsia formosa |
| CN103960203A (en) * | 2014-05-21 | 2014-08-06 | 中国农业科学院植物保护研究所 | Method for breeding encarsia sophia drones by means of eretmocerus hayati |
| CN104604809A (en) * | 2014-06-07 | 2015-05-13 | 云南省农业科学院农业环境资源研究所 | Biological control method using encarsia formosa for controlling tobacco whitefly |
| CN109526885A (en) * | 2018-12-19 | 2019-03-29 | 中国热带农业科学院环境与植物保护研究所 | A kind of expanding propagation method of Japan's food a red-spotted lizard aphid chalcid fly drone |
| CN110574733A (en) * | 2019-10-26 | 2019-12-17 | 中国农业科学院植物保护研究所 | Method for breeding male bees of young Aphidius avenae with Aphidius gossypii |
| CN110839596A (en) * | 2019-11-04 | 2020-02-28 | 中国农业科学院植物保护研究所 | Method for breeding male bees of young yellow Encarsia aphidicola by using aphidius gifuensis |
| CN117063759A (en) * | 2023-09-19 | 2023-11-17 | 吉林农业大学 | Method for breeding female bees of light yellow Enzus aphid based on spiral whiteflies as primary hosts |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111527950B (en) * | 2020-04-26 | 2021-11-23 | 无锡南长园林绿化工程有限公司 | Prevention and control method for goldenrain tree aphids |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1631125A (en) * | 2005-01-26 | 2005-06-29 | 北京市农林科学院 | Artificial progagation method for Encarsia formosa |
| CN101390505A (en) * | 2008-10-28 | 2009-03-25 | 北京市农林科学院 | Encarsia Sophia artificial propagation production method |
| CN101803588A (en) * | 2010-04-01 | 2010-08-18 | 衡水田益生防有限责任公司 | Method for breeding encarsia formosa all year round |
-
2011
- 2011-06-26 CN CN 201110173344 patent/CN102273432B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1631125A (en) * | 2005-01-26 | 2005-06-29 | 北京市农林科学院 | Artificial progagation method for Encarsia formosa |
| CN101390505A (en) * | 2008-10-28 | 2009-03-25 | 北京市农林科学院 | Encarsia Sophia artificial propagation production method |
| CN101803588A (en) * | 2010-04-01 | 2010-08-18 | 衡水田益生防有限责任公司 | Method for breeding encarsia formosa all year round |
Non-Patent Citations (1)
| Title |
|---|
| 李元喜 等: "烟粉虱两种寄生蜂生物学特性及寄主竞争关系研究", 《昆虫学报》, no. 7, 31 July 2008 (2008-07-31), pages 738 - 744 * |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102630647B (en) * | 2012-05-03 | 2014-05-07 | 中国农业科学院植物保护研究所 | Method for propagating Diglyphus isaea |
| CN102630647A (en) * | 2012-05-03 | 2012-08-15 | 中国农业科学院植物保护研究所 | Method for propagating Diglyphus isaea |
| CN102870742A (en) * | 2012-09-24 | 2013-01-16 | 中国农业科学院植物保护研究所 | Large-scale artificial feeding method for eretmocerus hayati |
| CN103283540A (en) * | 2013-06-26 | 2013-09-11 | 广西壮族自治区林业科学研究院 | Method for biological control over mangrove plants primary pest Aegiceras Chaetognatha tortricidae small leaf rollers |
| CN103385220B (en) * | 2013-07-25 | 2014-10-29 | 任伟 | Scale breeding method for encarsia formosa |
| CN103385220A (en) * | 2013-07-25 | 2013-11-13 | 任伟 | Scale breeding method for encarsia formosa |
| CN103960203A (en) * | 2014-05-21 | 2014-08-06 | 中国农业科学院植物保护研究所 | Method for breeding encarsia sophia drones by means of eretmocerus hayati |
| CN103960203B (en) * | 2014-05-21 | 2015-11-18 | 中国农业科学院植物保护研究所 | A kind of Hai Shi of utilization eretmocerus SP breeds the method for pale yellow grace aphid chalcid fly Xiong Feng |
| CN104604809A (en) * | 2014-06-07 | 2015-05-13 | 云南省农业科学院农业环境资源研究所 | Biological control method using encarsia formosa for controlling tobacco whitefly |
| CN109526885A (en) * | 2018-12-19 | 2019-03-29 | 中国热带农业科学院环境与植物保护研究所 | A kind of expanding propagation method of Japan's food a red-spotted lizard aphid chalcid fly drone |
| CN110574733A (en) * | 2019-10-26 | 2019-12-17 | 中国农业科学院植物保护研究所 | Method for breeding male bees of young Aphidius avenae with Aphidius gossypii |
| CN110839596A (en) * | 2019-11-04 | 2020-02-28 | 中国农业科学院植物保护研究所 | Method for breeding male bees of young yellow Encarsia aphidicola by using aphidius gifuensis |
| CN117063759A (en) * | 2023-09-19 | 2023-11-17 | 吉林农业大学 | Method for breeding female bees of light yellow Enzus aphid based on spiral whiteflies as primary hosts |
| CN117063759B (en) * | 2023-09-19 | 2024-02-23 | 吉林农业大学 | Method for breeding female bees of light yellow Enzus aphid based on spiral whiteflies as primary hosts |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102273432B (en) | 2012-12-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102273432B (en) | Method for producing drone of light yellow encarsia amicula by taking encarsia formosa gahan as breeding host | |
| CN101390505B (en) | Encarsia Sophia artificial propagation production method | |
| CN103477921B (en) | Method for comprehensive control of root knot nematode disease of potted ficus microcarpa | |
| CN106258755B (en) | A kind of implantation methods of the yellow jacket tree forest interplanting bletilla striata | |
| CN103385220A (en) | Scale breeding method for encarsia formosa | |
| CN105145490B (en) | A kind of method and its application of vegetable seedling breeding aphidius gifuensis | |
| CN104186265B (en) | A kind of blueberry list trunk Labor-saving cultivation shaping methods | |
| CN102334468A (en) | High-efficiency floating seedling Aphidius gifuensis propagation method | |
| CN103960203A (en) | Method for breeding encarsia sophia drones by means of eretmocerus hayati | |
| CN109479648A (en) | A kind of salt-soda soil organic paddy rice implantation methods | |
| CN109526885A (en) | A kind of expanding propagation method of Japan's food a red-spotted lizard aphid chalcid fly drone | |
| CN109089687A (en) | Borneol camphor tree cuttage and seedling culture propagation method | |
| KR20170000656A (en) | Breeding method of Haplothrips brevitubus [Karny] and controlling method for thrips using it | |
| CN105165313A (en) | Method for breeding aphidius gifuensis within short cycle | |
| CN103766324A (en) | Method for biologically preventing green plant bugs by peristenus spretus in winter jujube orchard | |
| KR100421779B1 (en) | Korean entomopathogenic nematode, steinernema carpocapsae gsn 1 and control method for insect | |
| CN111903618A (en) | Artificial cultivation method of Anagrus nilaparvatae (Anagrus nilaparvatae) | |
| CN101002529A (en) | Method for preventing and controlling cotton bollworm | |
| Jovicich et al. | Mite population and damage caused by broad mites (Polyphagotarsonemus latus [Banks]) infesting bell pepper (Capsicum annuum L.) at different seedling developmental stages | |
| CN109089698A (en) | A kind of rose high yield method for planting | |
| CN102972353A (en) | Three-dimensional scale mixed feeding method for ophraella communa and epiblema strenuana | |
| Khushvaqt et al. | Bioecology of orchard mites and the effectiveness of modern insecticides against them | |
| CN117063759B (en) | Method for breeding female bees of light yellow Enzus aphid based on spiral whiteflies as primary hosts | |
| CN105393993A (en) | Method for artificial mass-reproduction of Echinothrips americanus Morgan | |
| CN110679404B (en) | Method for preventing and controlling terrestrial water peanuts by plant substitution control and natural enemy synergy |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121226 Termination date: 20140626 |
|
| EXPY | Termination of patent right or utility model |