CN107182943B - A kind of method of interior mass rearing Bradysia fungus gnat - Google Patents
A kind of method of interior mass rearing Bradysia fungus gnat Download PDFInfo
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- CN107182943B CN107182943B CN201710350446.0A CN201710350446A CN107182943B CN 107182943 B CN107182943 B CN 107182943B CN 201710350446 A CN201710350446 A CN 201710350446A CN 107182943 B CN107182943 B CN 107182943B
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- 241001157791 Mycetophilus sp. HMR-1993 Species 0.000 title claims abstract description 76
- 241001494113 Bradysia Species 0.000 title claims abstract description 73
- 238000000034 method Methods 0.000 title claims abstract description 23
- 230000000384 rearing effect Effects 0.000 title claims abstract description 17
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 23
- 240000001462 Pleurotus ostreatus Species 0.000 claims abstract description 20
- 235000001603 Pleurotus ostreatus Nutrition 0.000 claims abstract description 20
- 235000007685 Pleurotus columbinus Nutrition 0.000 claims abstract description 19
- 238000004519 manufacturing process Methods 0.000 claims abstract description 11
- 239000011159 matrix material Substances 0.000 claims abstract description 7
- 238000011081 inoculation Methods 0.000 claims abstract description 6
- 239000001963 growth medium Substances 0.000 claims description 30
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 18
- 241000382353 Pupa Species 0.000 claims description 12
- 238000012258 culturing Methods 0.000 claims description 10
- 244000144987 brood Species 0.000 claims description 9
- 235000014655 lactic acid Nutrition 0.000 claims description 9
- 239000004310 lactic acid Substances 0.000 claims description 9
- 239000002609 medium Substances 0.000 claims description 9
- 238000012360 testing method Methods 0.000 claims description 9
- 230000012447 hatching Effects 0.000 claims description 7
- 235000013601 eggs Nutrition 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 241000607479 Yersinia pestis Species 0.000 claims description 4
- 239000000356 contaminant Substances 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 210000001015 abdomen Anatomy 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 230000032669 eclosion Effects 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 238000007790 scraping Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 230000017448 oviposition Effects 0.000 claims description 2
- 238000011534 incubation Methods 0.000 claims 1
- 238000009395 breeding Methods 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 230000001932 seasonal effect Effects 0.000 abstract description 3
- 241000238631 Hexapoda Species 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 6
- 239000001965 potato dextrose agar Substances 0.000 description 6
- 102000002322 Egg Proteins Human genes 0.000 description 5
- 108010000912 Egg Proteins Proteins 0.000 description 5
- 210000004681 ovum Anatomy 0.000 description 5
- 241000233866 Fungi Species 0.000 description 3
- 241000256095 Sciaridae Species 0.000 description 3
- 230000001418 larval effect Effects 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 240000006108 Allium ampeloprasum Species 0.000 description 2
- 235000005254 Allium ampeloprasum Nutrition 0.000 description 2
- 241000255925 Diptera Species 0.000 description 2
- 241000255932 Nematocera Species 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 238000004500 asepsis Methods 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 244000251953 Agaricus brunnescens Species 0.000 description 1
- 241000222532 Agrocybe Species 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241001157808 Mycetophilidae Species 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- 241000134360 Sciaroidea Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002431 foraging effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000029264 phototaxis Effects 0.000 description 1
- 238000004382 potting Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of methods of indoor mass rearing Bradysia fungus gnat, belong to plant protection and cultured insect technical field.The method raises Bradysia fungus gnat using the hypha of Pleurotus ostreatus of culture dish culture, realize successive propagation, include the steps that cultivating matrix manufacturing, hypha of Pleurotus ostreatus inoculated and cultured, the acquisition of Bradysia fungus gnat kind worm and continuously rearing, culture matrix manufacturing peace mushroom mycelium inoculation is completed on superclean bench.The present invention raises Bradysia fungus gnat using mycelia, avoids the shortcomings that being subject to seasonal restrictions by the raising of fresh host plant.Method for breeding of the invention is saved manually, easy to operate.A growth cycle for Bradysia fungus gnat only needs to operate 2-3 times, is greatly saved working hour.
Description
Technical field
The present invention relates to a kind of methods of indoor mass rearing Bradysia fungus gnat, belong to plant protection and cultured insect technology neck
Domain.
Background technique
Eye Sciaridae (Sciaridae) belongs to Diptera (Diptera) Nematocera (Nematocera) eye fungus gnat Superfamily
(Sciaroidea), type is abundant, widely distributed, likes moist dark environment, adaptable.The larva of most of population
It lives in the excrement of withered trees, fungi and animal, some larvas also live in cave, vegetable plot, in greenhouse.Eye fungus gnat is raw
Period living is usually 3~4 weeks, and Life of Adult 3~5 days, the ovum phase 4~7 days, 9~l of larval phase 7 days, pupa time 3~5 days；Female adult one
As can lay eggs 70~200, in gap of the ovum fecund between soil seam, plant base portion and leaf sheath gap.
Bradysia fungus gnat (Bradysia spp.) is that one of more, category for endangering most serious, the whole world are studied in a Sciaridae
Known to have 455 kinds, what China had recorded has 131 kinds；It is widely distributed, foraging territories are wide, the various vegetables that can cause harm and edible mushroom,
It can lead to crop total crop failure when serious.Cluster is overwintering mostly in plant roots and stems and root surrounding soil gap for larva, the Winter in North China phase
For mid or late October~mid or late November, Yu Yinian mid or late March starts to pupate, and April, early and middle ten days reached emergence peak；It gets in south
Teletostage is mid or late December, and Yu Yinian late Febuary starts to pupate, and reaches emergence peak mid-March.4~June, late September~11
Month worm amount is more, is hazard boom in 2 season of spring and autumn, and 7~August causes worm amount to die-off because of high temperature and drought.
The main feeding of Bradysia fungus gnat larva is caused harm the under ground portion of plant and the mycelium of edible mushroom, fructification etc., may be used also
Propagate disease；There is adult stronger phototaxis to be connected between causing though not feeding can propagate pathogen, nematode and mite class etc.
Evil.Bradysia fungus gnat individual is small, reproduction speed is fast, it is hidden to cause harm, and prevention and treatment is difficult and host range is wide, has become in agricultural production
The problem of urgent need to resolve.Especially in Edible Fungi, Bradysia fungus gnat can cause harm a variety of foods such as oyster mushroom, agrocybe, agaricus bisporus
With bacterium, the yield and quality of edible mushroom is seriously affected.And the current domestic registered insecticide that can be used on edible mushroom
It is less；The edible fungi growth period is short, and unreasonable for a long time easily causes product excessive pesticide residues using pesticide, generates pest anti-
Pharmacological property.Therefore need to carry out testing sieve select to the edible mushroom preferable biological pesticide of Bradysia fungus gnat effect and efficiently, low toxicity, it is low residual
The chemical pesticide stayed carrys out Instructing manufacture medication.And in Chemicals and integrated control research, need that a large amount of worm ages are consistent, development
Neat larva is as test worm.The artificial feeding of Bradysia fungus gnat at present mostly using fresh host plant or in vitro rhizome as feed,
There are season limit and higher cost, the foods such as man-made feeds or peanut, potato, soybean is also used to carry out Scale dependency for feed
Bradysia fungus gnat, but operation is more complex, it is time-consuming.
Summary of the invention
For the method for breeding of existing Bradysia fungus gnat, needed to better meet the test of edible mushroom Bradysia fungus gnat research
It asks, realizes the extensive raising of Bradysia fungus gnat, inventor passes through long-term experiment, provides a kind of using hypha of Pleurotus ostreatus as feed
The method for raising Bradysia fungus gnat.The present invention can be realized the anniversary mass rearing of Bradysia fungus gnat, and easy to operate, save work
When, save the cost, be suitble to obtain in laboratory and largely develop consistent Bradysia fungus gnat larva for experimental study.
A kind of method of indoor mass rearing Bradysia fungus gnat provided by the invention, is raised using the hypha of Pleurotus ostreatus of culture dish culture
Bradysia fungus gnat is supported, realizes successive propagation, including culture matrix manufacturing-hypha of Pleurotus ostreatus culture-Bradysia fungus gnat kind worm acquisition-Bradysia fungus gnat
The step of continuously rearing, wherein culture matrix manufacturing peace mushroom cultural hypha is completed on superclean bench.Specific step is as follows:
Step 1: PDA culture medium production.
(1) it weighs finished product PDA culture medium 39g, 1000mL water to pour into beaker, bevelling stirring in side obtains culture medium solution；
Preferably, the finished product PDA culture medium and water can heat in the water-bath of boiling, promote dissolution.
(2) culture medium solution dissolved is dispensed into 4 500mL triangular flasks, seals mouth with sealed membrane and rubber band,
121 DEG C sterilize 20 minutes.
(3) when temperature drops to 50~60 DEG C after the completion of sterilizing, lactic acid is added in culture medium solution.Every 1000mL culture medium
200 μ L of lactic acid is added in solution, shakes up.The mass percent concentration of the lactic acid is 25%.
(4) culture medium solution for having added lactic acid is poured into the culture dish of diameter 9cm, culture medium solution in culture dish is blown
After dry, it is spare to obtain dehydrated medium.
Step 2: hypha of Pleurotus ostreatus inoculation, culture.
(1) actication of culture.With the hypha of Pleurotus ostreatus in oese scraping test tube, it is inoculated into the culture dish with dehydrated medium
On.Inoculated culture dish is placed in 25 DEG C of incubators, 24 hours dark culturings, 5 days or so mycelia cover with and can be expanded
Culture.
(2) squamous subculture.The dehydrated medium for covering with mycelia in culture dish is divided into several fritters, is inoculated into new tool
Have on the culture dish of dehydrated medium, 24 hours dark culturings are carried out in 25 DEG C of incubators.Culture dish after covering with mycelia is put
Enter (4~6 DEG C of temperature) stored refrigerated in refrigerator, is raised for Bradysia fungus gnat.
Note: stringent asepsis requirements are paid attention in culture matrix manufacturing peace mushroom silk seeded process, avoid living contaminants.
Step 3: Bradysia fungus gnat kind worm obtains.
The mushroom rod to be caused harm by Bradysia fungus gnat is collected in mushroom shed, Bradysia fungus gnat four-age larva or pupa in picking mushroom rod are put
Enter in the culture dish for covering with mycelia in step 2 after squamous subculture；The culture dish for accessing Bradysia fungus gnat is put into 25 DEG C of incubators
Middle dark culturing, after four-age larva culture 5~7 days, after pupa culture 3~4 days, adult eclosion.The adult after sprouting wings is allowed to cover with
Pangamy is laid eggs in the culture dish of mycelia, and the first brood of larvae of hatching is as kind of a worm.
It can also be caused harm with pest sucking device in Bradysia fungus gnat and collect abdomen more bulging female adult and male worm in serious mushroom shed, be put into step
Raising in the culture dish for covering with mycelia in rapid two after squamous subculture, it is 1~2 day dark in 25 DEG C of incubators after Adult worms producting eggs,
The first brood of larvae of hatching is as kind of a worm.
Step 4: Bradysia fungus gnat continuously rearing.
(1) the Bradysia fungus gnat the in the culture dish raising step 3 for covering with mycelia after the squamous subculture prepared with step 2
Generation larva is kind of a worm, is pupated within 8~12 days after larvae hatch.
(2) first brood of larvae of Bradysia fungus gnat i.e. kind of worm by the hypha of Pleurotus ostreatus feeding in the culture dish for covering with mycelia it is complete after,
The culture medium with larva is divided into several pieces with scalpel or pincet, is respectively put into the new culture dish for covering with mycelia,
Continue to raise.
(3) after Bradysia fungus gnat pupates, pupa is chosen in the new culture dish for covering with mycelia with hooking pen, it is numerous to carry out subculture
It grows.
Note: under the conditions of 25 DEG C, pupa time and ovum phase are distinguished 3~4 days, larval phase 8~12 days, can be chosen not according to test demand
Bradysia fungus gnat larva of the same period is tested.
The present invention has the advantage that
(1) present invention raises Bradysia fungus gnat using mycelia, avoids and is subject to seasonal restrictions by the raising of fresh host plant
Disadvantage.
(2) method for breeding of the invention is saved artificial, easy to operate.A growth cycle for Bradysia fungus gnat only needs to operate
2~3 times, it is greatly saved working hour.
Detailed description of the invention
Fig. 1 is the method flow diagram of indoor mass rearing Bradysia fungus gnat provided by the invention.
Specific embodiment
The present invention is described in detail with reference to the accompanying drawings and examples.
The present invention provides a kind of method of indoor mass rearing Bradysia fungus gnat, and process as shown in Figure 1 specifically includes following step
It is rapid:
Step 1: PDA culture medium production.
(1) electronic scale weighs finished product PDA culture medium (potato dextrose agar) 39g, pours into 2000mL beaker.It takes
1000mL water pours into beaker, the stirring of side bevelling.It can be placed in boiling water bath and heat, promote dissolution.
(2) culture medium dissolved is dispensed into 4 500mL triangular flasks, seals mouth with sealed membrane and rubber band, 121 DEG C
Sterilizing 20 minutes.
(3) when temperature drops to 50~60 DEG C after the completion of sterilizing, culture medium is taken out, is operated in superclean bench, cream is added
Acid.200 μ L of lactic acid is added in every 1000mL culture medium, shakes up.The mass percent concentration of the lactic acid is 25%.
(4) in superclean bench, the culture medium for having added lactic acid is poured into the culture dish of diameter 9cm, culture medium drying
Obtain having the culture dish of dehydrated medium spare afterwards.
Step 2: hypha of Pleurotus ostreatus inoculation, culture.
(1) actication of culture.With the hypha of Pleurotus ostreatus in oese scraping test tube in superclean bench, it is inoculated into step 1
On culture dish with dehydrated medium, guarantees gnotobasis, avoid living contaminants.Inoculated culture dish is placed on 25 DEG C of trainings
It supports in case, 24 hours dark culturings, 5 days or so mycelia cover with and can expand culture.
(2) squamous subculture.The culture medium for covering with mycelia is divided into several fritters in superclean bench, is inoculated into new
On dehydrated medium, 24 hours dark culturings are carried out in 25 DEG C of incubators.The culture dish for covering with mycelia, which is put into refrigerator, to be refrigerated
It saves, is raised for Bradysia fungus gnat.
Note: stringent asepsis requirements are paid attention in culture matrix manufacturing peace mushroom silk seeded process, avoid living contaminants.
Step 3: Bradysia fungus gnat kind worm obtains.
The Bradysia fungus gnat kind worm obtains through any one realization in the following two kinds mode:
(1) mushroom rod to be caused harm by Bradysia fungus gnat is collected in mushroom shed, takes back laboratory.Bradysia fungus gnat four in picking mushroom rod
Instar larvae or pupa, are put into the culture dish for covering with mycelia；The culture dish for covering with mycelia for accessing Bradysia fungus gnat is put into 25 DEG C of trainings
Dark culturing in case is supported, after four-age larva culture 5~7 days, after pupa culture 3~4 days, adult eclosion.The adult after sprouting wings is allowed to exist
It covers with pangamy in the culture dish of mycelia to lay eggs, the first brood of larvae of hatching is as kind of a worm.
(2) it can also be caused harm with pest sucking device in Bradysia fungus gnat and collect abdomen more bulging female adult and male worm in serious mushroom shed, put
Enter raising in the culture dish for covering with mycelia in step 2 after squamous subculture, in 25 DEG C of incubators after dark culturing 1~2 day at
Worm oviposition, the first brood of larvae of hatching is as kind of a worm.
Step 4: Bradysia fungus gnat continuously rearing.
(1) with the culture dish raising Bradysia fungus gnat first brood of larvae for covering with mycelia, i.e., the kind worm obtained in step 3, larva
It pupates within 8~12 days after hatching.
(2) Bradysia fungus gnat larva by the hypha of Pleurotus ostreatus feeding in the culture dish for covering with mycelia it is complete after, with scalpel or small tweezer
Culture medium with larva is divided into several pieces by son, is respectively put into the new culture dish for covering with mycelia, is continued to raise.
(3) after Bradysia fungus gnat pupates, pupa is chosen in the new culture dish for covering with mycelia with hooking pen, it is numerous to carry out subculture
It grows.
Note: under the conditions of 25 DEG C, the pupa time of Bradysia fungus gnat and ovum phase are distinguished 3~4 days, larval phase 8~12 days, can be according to test
The Bradysia fungus gnat larva that demand chooses different times is tested.
Under the conditions of 25 DEG C, Bradysia fungus gnat is raised with potting leek seedling, a generation (from ovum to adult) is completed and needs 23~25
It, every basin leek seedling can get 150~200 larvas when accessing 5 pairs of Bradysia fungus gnat adults；It is that feed raising is slow with fresh potato
Eye fungus gnat completes a generation and needs 20~23 days, and every box can get 200~300 larvas when accessing 5 pairs of Bradysia fungus gnat adults.This
The method that invention provides raises Bradysia fungus gnat, completes a generation and needs 14~20 days, in each culture dish 5 pairs of Bradysia fungus gnats of access at
It can get 400~500 larvas when worm.
Method feeding materials provided by the invention are hypha of Pleurotus ostreatus, are easy to get, without seasonal limitation；Raising container is
Culture dish takes up space small, can make mass propgation ware in incubator simultaneously and carry out anniversary breeding；It is compared with other methods, the present invention
The method of offer is easy to operate, saving working hour, and the period is short, high survival rate, can get and largely develops consistent larva for test.
Claims (3)
1. a kind of method of interior mass rearing Bradysia fungus gnat, it is characterised in that be directly appearance with the culture dish for covering with hypha of Pleurotus ostreatus
Device raises Bradysia fungus gnat, including PDA culture medium production, and hypha of Pleurotus ostreatus inoculation, culture, Bradysia fungus gnat kind worm obtains and Bradysia fungus gnat
The step of continuously rearing；
It is specific as follows；
Step 1: PDA culture medium production；
(1) it weighs finished product PDA culture medium 39g, 1000mL water to pour into beaker, bevelling stirring in side obtains culture medium solution；
(2) culture medium solution dissolved is attached in triangular flask, seals mouth with sealed membrane and rubber band, 121 DEG C sterilize 20 minutes；
(3) when temperature drops to 50~60 DEG C after the completion of sterilizing, lactic acid is added in culture medium solution；
(4) culture medium solution of lactic acid will have been added to pour into culture dish, after culture medium solution drying in culture dish, has obtained drying
Culture medium is spare；
Step 2: hypha of Pleurotus ostreatus inoculation, culture；
(1) it actication of culture: with the hypha of Pleurotus ostreatus in oese scraping test tube, is inoculated on the culture dish with dehydrated medium；
Inoculated culture dish is placed in 25 DEG C of incubators, 24 hours dark culturings；
(2) squamous subculture: being divided into several fritters for the dehydrated medium for covering with mycelia in culture dish, is inoculated into new having and does
On the culture dish of dry culture medium, 24 hours dark culturings are carried out in 25 DEG C of incubators；
Step 3: Bradysia fungus gnat kind worm obtains, using the following two kinds mode:
The mushroom rod to be caused harm by Bradysia fungus gnat is collected in mushroom shed, Bradysia fungus gnat four-age larva or pupa in picking mushroom rod are put into step
In the culture dish for covering with mycelia in rapid two after squamous subculture；The culture dish for accessing Bradysia fungus gnat is put into black in 25 DEG C of incubators
Dark culture, after four-age larva culture 5~7 days, after pupa culture 3~4 days, adult eclosion；The adult after sprouting wings is allowed to cover with mycelia
Culture dish in pangamy oviposition, the first brood of larvae of hatching is as kind of a worm；
Or caused harm with pest sucking device in Bradysia fungus gnat and collect abdomen more bulging female adult and male worm in serious mushroom shed, it is put into step 2
Raising in the culture dish for covering with mycelia after middle squamous subculture, the Adult worms producting eggs after dark culturing 1~2 day in 25 DEG C of incubators,
The first brood of larvae of hatching is as kind of a worm；Step 4: Bradysia fungus gnat continuously rearing；
(1) the Bradysia fungus gnat first generation in the culture dish raising step 3 for covering with mycelia after the squamous subculture prepared with step 2
Larva is kind of a worm, is pupated within 8~12 days after larvae hatch；
The first brood of larvae of Bradysia fungus gnat by the hypha of Pleurotus ostreatus feeding in the culture dish for covering with mycelia it is complete after, with scalpel or small tweezer
Culture medium with larva is divided into several pieces by son, is respectively put into the new culture dish for covering with mycelia, is continued to raise；
(2) after Bradysia fungus gnat pupates, pupa is chosen in the new culture dish for covering with mycelia with hooking pen, carries out successive propagation.
2. a kind of method of indoor mass rearing Bradysia fungus gnat according to claim 1, it is characterised in that: culture matrix manufacturing
With hypha of Pleurotus ostreatus inoculation, in incubation it is noted that aseptic condition, avoids living contaminants.
3. a kind of method of indoor mass rearing Bradysia fungus gnat according to claim 1, it is characterised in that: pass through step 1
It is stored refrigerated under the conditions of 4~6 DEG C need to be placed on the culture dish for covering with hypha of Pleurotus ostreatus that step 2 obtains, it is raised for Bradysia fungus gnat
With.
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| FR3110338A1 (en) * | 2020-05-20 | 2021-11-26 | Fungfeed | FOOD FOR INSECTS - BREEDING / FEEDING METHOD, INSECT LARVA AND ASSOCIATED FEED |
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| CN108990909B (en) * | 2018-05-25 | 2020-05-15 | 华南农业大学 | Method for trapping bradysia odoriphaga live insects and feeding bradysia odoriphaga live insects indoors in large quantities |
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| CN104798734B (en) * | 2015-05-19 | 2017-05-24 | 天津市植物保护研究所 | Method for feeding predatory coenosia larvae indoors |
| CN205667255U (en) * | 2016-06-14 | 2016-11-02 | 长江大学 | A kind of Bradysia odoriphaga adult assay device |
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| FR3110338A1 (en) * | 2020-05-20 | 2021-11-26 | Fungfeed | FOOD FOR INSECTS - BREEDING / FEEDING METHOD, INSECT LARVA AND ASSOCIATED FEED |
| EP3912476A3 (en) * | 2020-05-20 | 2022-02-23 | Fungfeed | Insect feed -method for farming/feeding, insect larva and associated food product |
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| CN107182943A (en) | 2017-09-22 |
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