CN110973074A - Breeding method and device for scale insects and living feed for natural enemy insects - Google Patents

Breeding method and device for scale insects and living feed for natural enemy insects Download PDF

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Publication number
CN110973074A
CN110973074A CN202010025635.2A CN202010025635A CN110973074A CN 110973074 A CN110973074 A CN 110973074A CN 202010025635 A CN202010025635 A CN 202010025635A CN 110973074 A CN110973074 A CN 110973074A
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pumpkin
mealybugs
water
citrus
breeding
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Chinese (zh)
Inventor
夏鹏亮
黄勇
任晓红
尹虎成
解晓菲
彭五星
师超
乔保明
全琳
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ENSHI CO., LTD., HUBEI TOBACCO Co.,Ltd.
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夏鹏亮
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/05Fruit crops, e.g. strawberries, tomatoes or cucumbers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/12Supports for plants; Trellis for strawberries or the like
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/90Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms

Abstract

The invention is suitable for the technical field of insect breeding, and provides a breeding method and a breeding device for citrus mealybugs and a living feed for natural enemy insects. The method for breeding the scale insects comprises the following steps: injecting water into the culture chamber and placing at least one set of support members in the water; placing at least one layer of pumpkin on the support member and keeping the pumpkin out of contact with water; inoculating the eggs of the mealybugs to the pumpkins for cultivation. According to the method for breeding the scale insects, the host pumpkin adopted by the method is hard and thick, and is not easy to mildew after being parasitized by the scale insects, so that the scale insects can be bred on the pumpkin for a long time, and the scale insects can be bred at high yield conveniently. In addition, because the growth and the propagation speed of the citrus mealybugs on the pumpkins are high, the pumpkins are used as hosts of the citrus mealybugs, and the breeding efficiency of the citrus mealybugs can be obviously improved.

Description

Breeding method and device for scale insects and living feed for natural enemy insects
Technical Field
The invention belongs to the technical field of insect breeding, and particularly relates to a breeding method and a breeding device for citrus mealybugs and a living feed for natural enemy insects.
Background
The Citrus chalicerus (Planococcus Citri) is an insect of the genus Ericerus belonging to the family Lecanidae. The main harmful crops of the citrus mealybugs are as follows: citrus, apple, grape, longan, pineapple, pomegranate, loquat, coconut, cashew, olive, pear, persimmon, fig, mulberry, tobacco, and the like.
Although the citrus mealybugs can parasitize on the citrus to be propagated, the citrus mealybugs are easy to rot after being parasitized by the citrus mealybugs, so that the citrus mealybugs are inconvenient to breed with high yield by the citrus.
Disclosure of Invention
The embodiment of the invention aims to provide a method for breeding a scale insect and a scale insect, and aims to solve the problems in the background art.
The embodiment of the invention is realized in such a way that the method for breeding the citrus mealybugs comprises the following steps:
the pumpkin is used as a host, and eggs or larvae of the mealybugs are inoculated to the pumpkin for cultivation.
As another preferable scheme of the embodiment of the invention, the cultivation method further comprises the following steps:
the pumpkin serving as the host is placed above the water surface by taking water as isolation, and the pumpkin is not contacted with the water, so that the pumpkin is prevented from rotting due to soaking in the water, and the negative influence of the water on the colonization of the mealybugs. As another preferable scheme of the embodiment of the invention, the cultivation method specifically comprises the following steps:
injecting water into the culture room, and placing at least one group of supporting pieces in the water, wherein the supporting pieces are made of waterproof materials;
placing at least one layer of pumpkin on the supporting member, and preventing the pumpkin from contacting with water, avoiding the pumpkin from rotting due to soaking in water, and reducing the negative effect of water on the colonization of the citrus mealybugs. Inoculating the eggs or larvae of the scale canicillium citrosum to the pumpkin for cultivation.
As another preferable scheme of the embodiment of the present invention, the step of inoculating the eggs of the mealybugs to the pumpkins for cultivation specifically includes:
picking out oocysts at the tail of the scale canicillium citrinum, taking out eggs (red) in the oocysts, and uniformly coating the taken eggs on the surface of the pumpkin.
As another preferable scheme of the embodiment of the present invention, the step of inoculating the larvae of the scale canicola to the pumpkin for cultivation specifically comprises:
the pumpkin with the citrus mealybugs is stacked on the pumpkin with the citrus mealybugs.
Another object of the embodiments of the present invention is to provide a breeding device for citrus mealybugs, which includes:
a culture room; the breeding chamber is filled with water, is made of waterproof materials and can be replaced by a large plastic tray;
at least one squash, to host the scale insect;
at least one set of supports for supporting the pumpkins so that the pumpkins do not come into contact with the water in the cultivation room; the support is arranged in the water in the culture room; the pumpkin is arranged on the supporting piece.
As another preferable scheme of the embodiment of the invention, the water level in the culture room is lower than the height of the support.
As another preferable scheme of the embodiment of the invention, the height of the water surface in the culture room is 1-5 cm.
Another object of an embodiment of the present invention is to provide a live feed for natural enemy insects, which comprises a population of citrus mealybugs bred by the breeding method.
As another preferable mode of the embodiment of the invention, the natural enemy insect is a ladybug natural enemy insect.
According to the method for breeding the scale insects, the host pumpkin adopted by the method is not easy to mildew after being parasitized by the scale insects, so that the scale insects can be bred on the pumpkin for a long time, and the scale insects can be bred at high yield conveniently. In addition, because the growth and the propagation speed of the citrus mealybugs on the pumpkins are high, the pumpkins are used as hosts of the citrus mealybugs, and the breeding efficiency of the citrus mealybugs can be obviously improved.
In addition, in the research process, the citrus mealybugs cannot fly or swim, and can only walk by legs except for being adsorbed on the surfaces of hosts to suck juice most of the time, so that the citrus mealybugs can be effectively prevented from escaping by adopting water as isolation in the embodiment of the invention.
Drawings
Fig. 1 is a schematic structural view of a breeding device for citrus mealybugs according to embodiment 1 of the present invention.
In the figure: 1-pumpkin; 2-a culture room; 3-water; 4-a support member.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The embodiment of the invention provides a method for breeding a scale insect, which comprises the following steps:
pumpkin is used as a host, water is used as isolation, the pumpkin used as the host is placed above the water surface, the pumpkin is not contacted with the water through a supporting piece, and then eggs or larvae of the mealybugs are inoculated on the pumpkin for cultivation. Specifically, the oocysts at the tail of the citrus mealybugs can be picked, eggs (red) in the oocysts are taken out, and the taken eggs are uniformly coated on the surface of the pumpkin; in addition, the pumpkin with the citrus mealybugs can be stacked on the pumpkin with the citrus mealybugs for cultivation.
Example 1
As shown in fig. 1, this embodiment provides a device for cultivating citrus mealybugs, which includes:
a culture room 2; water 3 is arranged in the culture room;
at least one pumpkin 1, which is used as a host of the mealybugs;
at least one set of supports 4 for supporting the pumpkin 1 so that the pumpkin 1 does not come into contact with the water 3 in the cultivation room 2; the support 4 is arranged in the water 3 inside the culture chamber 2; the pumpkin 1 is arranged on the supporting piece 4, and the supporting piece 4 is made of waterproof materials.
Specifically, the breeding room 2 can adopt a disc-shaped structure made of waterproof materials, or adopts a seedling raising water pool with the specification of 70cm multiplied by 12 cm; 1 part of pumpkin is the common pumpkin sold on the market, and a plurality of pumpkins 1 can be placed or stacked in multiple layers; the supporting member 4 can be made of a building plate brick or a stone which is commonly available on the market, and the upper surface of the supporting member needs to be smooth and flat so as to be convenient for placing the pumpkin 1.
As another preferable scheme of the embodiment of the invention, the water level in the culture room 2 is lower than the height of the support 4, so as to ensure that the water 3 can be prevented from contacting with the water when being placed on the support 4, therefore, the mealybugs can grow on all the surfaces of the pumpkins 1, and the problem that the pumpkins 3 are easy to mildew on the water can be prevented. Specifically, the height of the water surface in the culture room 2 can be 1-5 cm.
Example 2
The embodiment provides a method for breeding a mealybugs using the breeding device provided in embodiment 1, which specifically includes the following steps:
(1) water is poured into the breeding chamber 2, and a plurality of groups of supporting pieces 4 are placed in the water 3 to ensure that the upper surface of the citrus mealybugs is exposed to the water surface.
(2) A plurality of layers of squash 1 are placed on the support 4 and the squash 1 is kept out of contact with the water 3.
(3) Inoculating the eggs or larvae of the scale canicillium citrosum to the pumpkin for cultivation.
(4) Because the colony of the scale mealybugs expands, the scale mealybugs spread from the lower part to the upper part and spread to the top end and then spread all around, the seeding is started from the bottom layer pumpkin 1, and the evenly-expanded scale mealybugs colony can be obtained. Specifically, the oocysts at the tail of the citrus mealybugs are picked up, eggs (red) in the oocysts are dipped by a writing brush, and then the dipped eggs are uniformly smeared on the surface layer of the bottom layer pumpkin 1, wherein the inoculation amount of the citrus mealybugs can be controlled to be 10 eggs/cm2. In addition, when the citrus mealybugs grow on the bottom layer pumpkin 1, the new pumpkin without the citrus mealybugs can be directly stacked on the pumpkin with the citrus mealybugs (the pumpkin with the citrus mealybugs can be cleaned by a writing brush in advance to remove the citrus mealybugs which are easy to be killed), and the natural inoculation can be completed after 24 hours.
(5) After the citrus mealybugs are covered on the surface layer of the pumpkin 1, the citrus mealybug communities on the surface layer of the pumpkin 1 can be scraped by using a writing brush or thick paper so as to finish the cultivation of the citrus mealybugs. Wherein, because the cortex of the scale canicillium citrosum is soft and has large water content, the bred scale canicillium citrosum community can be directly used as a feed to feed natural enemy insects such as harmonia axyridis or coccinella septempunctata. In addition, because the citrus mealybugs grow in a colony mode, colonies of the citrus mealybugs are stacked together in the culture process, and the citrus mealybugs need to be frequently taken out to reduce the thickness of the colonies, so that the growth and propagation of the mealybugs at the bottom layer are facilitated.
It should be noted that, because the eggs of the citrus mealybugs can spread with the wind and can harm the surrounding crops, the cultivation room 2 can be placed in an indoor windless environment, and cannot be placed in an outdoor ventilated environment. In addition, the growth efficiency of the citrus mealybugs can be influenced by exposure to the sun and rain, and the pumpkins are easy to mildew, so that the cultivation room 2 cannot be placed in the open air. In addition, because the pumpkin after being washed by water is easy to rot, the surface of the pumpkin before being used is not washed by water as much as possible, and the pumpkin can be dried in advance if necessary.
Comparative example 1
This comparative example provides a method for breeding the scale insects of citrus, which is the same as that of example 2, except that citrus is used instead of pumpkin as the host of the scale insects of citrus.
Comparative example 2
This comparative example provides a method for breeding a scale insect of citrus, which is the same as that of example 2, except that a grapefruit is used instead of a pumpkin as a host of the scale insect.
Comparative example 3
This comparative example provides a method for breeding a scale insect of citrus, which has the same steps as those of example 2, except that orange is used instead of pumpkin as a host for the scale insect.
Comparative example 4
This comparative example provides a method for breeding a scale insect of citrus, which has the same steps as those of example 2, except that apples are used instead of pumpkins as hosts of the scale insect.
Comparative example 5
This comparative example provides a method for breeding a scale insect of citrus, which has the same steps as those of example 2, except that pears are used instead of pumpkins as hosts of the scale insect.
The citrus mealybugs are cultured according to the culture methods provided by the embodiment 2 and the comparative examples 1 to 5 respectively, and then the rotting time of each host and the time for the citrus mealybugs to be covered on the surface are observed and counted (the inoculation amount of the citrus mealybugs on each host is 10 eggs/cm)2) The statistical results are shown in table 1 below.
TABLE 1
Figure DEST_PATH_IMAGE002
As can be seen from Table 1 above, although the citrus mealybugs can be parasitic on the citrus, the citrus is particularly easy to rot, the citrus starts to mold about 5 days later, and the citrus cannot be further tested due to excessive rot after 10 days later. In addition, compared with the pomelos, the oranges, the apples and the pears, the pumpkins adopted in the embodiment of the invention are not easy to mildew and rot after being parasitized by the scale insects, and the scale insects can be distributed on the whole surface after being cultured for about 20 days. Therefore, the breeding method provided by the embodiment of the invention is convenient for breeding the scale insects with high efficiency and high yield.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims (10)

1. The method for breeding the citrus mealybugs is characterized by comprising the following steps of:
the pumpkin is used as a host, and eggs or larvae of the mealybugs are evenly inoculated to the pumpkin for cultivation.
2. The method for cultivating the citrus mealybugs according to claim 1, further comprising the steps of:
the pumpkin as host is put on the water surface with water as isolation and is not contacted with water.
3. The method for breeding the citrus mealybugs according to claim 2, characterized by comprising the following steps:
injecting water into the culture room, and placing at least one group of supporting pieces in the water, wherein the supporting pieces are made of waterproof materials;
placing at least one layer of squash on the support member such that the squash is not in contact with water;
and uniformly inoculating the eggs or larvae of the scale canicillium citrosum to the pumpkin for cultivation.
4. The method for cultivating the citrus mealybugs according to claim 1 or 3, wherein the step of inoculating the eggs of the citrus mealybugs to the pumpkins for cultivation specifically comprises:
picking out oocysts at the tail of the scale canicillium citrinum, taking out eggs in the oocysts, and uniformly coating the taken eggs on the surface of the pumpkin.
5. The method for cultivating the citrus mealybugs according to claim 1 or 3, wherein the step of inoculating the larvae of the citrus mealybugs to the pumpkins for cultivation comprises the following steps:
the pumpkin with the citrus mealybugs is stacked on the pumpkin with the citrus mealybugs.
6. The utility model provides a breeding device of oranges and tangerines mealybugs which characterized in that includes:
a culture room; the breeding chamber is internally provided with water and is made of waterproof materials;
at least one squash, to host the scale insect;
at least one set of supports for supporting the pumpkins so that the pumpkins do not come into contact with the water in the cultivation room; the support is arranged in the water in the culture room; the pumpkin is arranged on the supporting piece.
7. The apparatus for cultivating the citrus mealybugs according to claim 6, wherein the water level in the cultivation room is lower than the height of the supporting members.
8. The device for cultivating the citrus mealybugs according to claim 6, wherein the height of the water surface in the cultivation room is 1-5 cm.
9. A live feed for natural enemy insects, which comprises the colony of Lecanicillium citrosum bred by the breeding method according to any one of claims 1 to 5.
10. The live feed for natural enemy insects as claimed in claim 9, wherein the natural enemy insects are ladybug-type natural enemy insects.
CN202010025635.2A 2020-01-10 2020-01-10 Breeding method and device for scale insects and living feed for natural enemy insects Pending CN110973074A (en)

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Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007325541A (en) * 2006-06-08 2007-12-20 Arysta Lifescience Corp Apparatus for breeding natural enemy insect and method for using the same
CN102084850A (en) * 2010-12-05 2011-06-08 天津出入境检验检疫局动植物与食品检测中心 Method for breeding dysmicoccus neobrevipes
CN102144619A (en) * 2011-04-13 2011-08-10 云南省热带作物科学研究所 Artificial propagation method of diversinervus elegans silvestri
EP2612551A1 (en) * 2012-01-04 2013-07-10 Koppert B.V. Mite composition comprising a predatory mite and immobilized prey contacted with a fungus reducing agent and methods and uses related to the use of said composition
CN103782969A (en) * 2014-03-03 2014-05-14 广西壮族自治区林业科学研究院 Method for breeding trichogramma pintoi indoors and corcyra cephalonica raising fodder
CN104365557A (en) * 2014-09-19 2015-02-25 中山大学 Artificial rearing, breed conservation and breeding production method for cryptolaemus montrouzieri mulsant
CN107529740A (en) * 2015-04-13 2018-01-02 昆虫公司 Method for breeding insects
CN108124822A (en) * 2017-12-29 2018-06-08 中国热带农业科学院南亚热带作物研究所 The method and apparatus for raising the clean mealybug of pineapple
CN109526885A (en) * 2018-12-19 2019-03-29 中国热带农业科学院环境与植物保护研究所 A kind of expanding propagation method of Japan's food a red-spotted lizard aphid chalcid fly drone

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007325541A (en) * 2006-06-08 2007-12-20 Arysta Lifescience Corp Apparatus for breeding natural enemy insect and method for using the same
CN102084850A (en) * 2010-12-05 2011-06-08 天津出入境检验检疫局动植物与食品检测中心 Method for breeding dysmicoccus neobrevipes
CN102144619A (en) * 2011-04-13 2011-08-10 云南省热带作物科学研究所 Artificial propagation method of diversinervus elegans silvestri
EP2612551A1 (en) * 2012-01-04 2013-07-10 Koppert B.V. Mite composition comprising a predatory mite and immobilized prey contacted with a fungus reducing agent and methods and uses related to the use of said composition
CN103782969A (en) * 2014-03-03 2014-05-14 广西壮族自治区林业科学研究院 Method for breeding trichogramma pintoi indoors and corcyra cephalonica raising fodder
CN104365557A (en) * 2014-09-19 2015-02-25 中山大学 Artificial rearing, breed conservation and breeding production method for cryptolaemus montrouzieri mulsant
CN107529740A (en) * 2015-04-13 2018-01-02 昆虫公司 Method for breeding insects
CN108124822A (en) * 2017-12-29 2018-06-08 中国热带农业科学院南亚热带作物研究所 The method and apparatus for raising the clean mealybug of pineapple
CN109526885A (en) * 2018-12-19 2019-03-29 中国热带农业科学院环境与植物保护研究所 A kind of expanding propagation method of Japan's food a red-spotted lizard aphid chalcid fly drone

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