CN103858828A - Method for breeding neoseiulus californicus through apricot blossom pollen - Google Patents

Method for breeding neoseiulus californicus through apricot blossom pollen Download PDF

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CN103858828A
CN103858828A CN201410117438.8A CN201410117438A CN103858828A CN 103858828 A CN103858828 A CN 103858828A CN 201410117438 A CN201410117438 A CN 201410117438A CN 103858828 A CN103858828 A CN 103858828A
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california
culture dish
pollen
mite
breeding
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李庆
吴双
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Abstract

The invention discloses a method for breeding neoseiulus californicus through apricot blossom pollen. The method includes the steps that a mature and thick sword bean leaf is selected and washed clean and is then placed into a breeding box with the back facing upwards, and tetranychus cinnabarinus and the neoseiulus californicus are put to the leaf to be bred as an insect source; a layer of cotton is applied to the bottom of a culture dish with the diameter of 9 cm and wetted with water, and the selected thick sword bean leaf is cut into a circle and closely attached to the wet cotton to be closely surrounded by the cotton strips and is then moistened; a certain number of neoseiulus californicus eggs laid within 24 h are selected and placed on the circular leaf, the culture dish is filled with a small amount of apricot blossom pollen and is then sealed through a preservative film, the small holes are punched in the preservative film, the growth conditions of the predatory mites are observed once every 12 h, and the pollen is replaced once every 24 h; the selected eggs on the circular leaf are incubated within 24 h to 48 h and bred to 6-7 d, and mating is carried out when the mating phenomenon occurs; female mites having been mated are placed into an independent culture dish to be bred, the egg laying amount is observed once every 12 h, and laid eggs are picked out and placed into another culture dish and can also be incubated normally, feed, grow and be mated to lay eggs.

Description

A kind of method that adopts the new little peaceful mite in apricot pollen breeding California
Technical field
The invention belongs to new little peaceful mite propagation technique field, California, relate in particular to a kind of method that adopts the new little peaceful mite in apricot pollen breeding California.
Background technology
The new little peaceful mite in California can prey on the multiple harmful mites such as Tetranychus cinnabarinus, oranges and tangerines beginning tetranychid, panonychus citri, and using predatory mite to control pest mites is significant for reducing residue of pesticide and green agriculture.This mite is picked up from Chengdu, Sichuan Pujiang by the 2009 grades of postgraduate Qin Guiyong of Agricultural Entomology and Pest Control specialty of Sichuan Agricultural University; tentatively assert that by Plant Protection institute, Chinese Academy of Agricultral Sciences Xu research institute of learning to farm this mite is domestic New Records, and identify that this mite belongs to Phytoseiidae Phytoseiidae, little Neosciulus Neoseiulus.
Predatory Mites carry out that biological control uses abroad relatively extensively and technology also comparatively ripe.China is being also in the primary stage aspect application Predatory Mites control evil mite, also more limited in the area of application.Comparatively ripe kind has amblyseius cucumeris oudeman A.cucumeris and Amblyseius barkeri A.barkeri, but at Sichuan province amblyseius cucumeris oudeman owing to not surviving the winter, need to manually discharge every year, expenses for prevention and control increases, waste time and energy.And the new little peaceful mite in California has the certain control ability of evil mite, and suitable to Sichuan Overwintering.
In general breeding of Predatory Mites have natural prey method for breeding, substitute prey method for breeding, pollen method for breeding, four kinds of approach of artificial feed method for breeding.Natural prey method for breeding ratio is easier to successfully, but but scale breeding time can run into many technical barriers, the reproductive process of natural prey is generally the cultivation of natural prey host plant, inoculates natural prey, multiple links such as inoculation Predatory Mites, separation packing, storage transportation.Wherein separate Predatory Mites and need to specifically wash mite machine, due to business, technique is maintained secrecy, and is difficult to obtain.While throwing in Predatory Mites, remaining harmful mite harm prevention target is also a very large risk.Be applicable to the wider Predatory Mites of recipe and substitute prey, and the new little peaceful mite in California does not belong to this.Artificial feed aspect, realizes already artificial feed abroad and raise the new little peaceful mite in California, and carry out batch production production, but equally because business reason has been carried out technical know-how, the pertinent literature that can find is all vague descriptions, critical data does not have.
The new little peaceful mite in California belongs to Phytoseiidae, and phytoseiid mite can utilize pollen as transition food conventionally.But not that each pollen is all applicable to Predatory Mites and takes food.The suitability of pollen is turned to three levels by we, worst one-level be Predatory Mites not with search for food.The second is to take food, but can not complete successive propagation, can not mate and oviposit.The third is to help Predatory Mites to complete whole Development History, can mate and oviposit, and ovum can be hatched smoothly.
Through Preliminary screening, more than ten, plant to filter out common pollen and severally can make the new little peaceful mite in California complete whole Development History, and the pollen of mate and oviposit smoothly.
Summary of the invention
The invention provides a kind of method that adopts the new little peaceful mite in apricot pollen breeding California, be intended in the situation that artificial feed are not also broken through at present, with the new little peaceful mite in apricot pollen raising California, make the new little peaceful mite in California can successive propagation, complete the whole history of life, mate and oviposit smoothly, quickening Predatory Mites carries out popularization and the enforcement of biological control.
The object of the present invention is to provide a kind of method that adopts the new little peaceful mite in apricot pollen breeding California, the method for this new little peaceful mite in employing apricot pollen breeding California comprises the following steps:
Step 1, chooses aging thicker blade, cleans, and puts into and raises box, upward, access Tetranychus cinnabarinus, in the time that Tetranychus cinnabarinus population reaches 200 left and right at the back side, with small size writing brush access California genus amblyseius berlese, with stereomicroscope observation Tetranychus cinnabarinus and California genus amblyseius berlese, grasp population and change;
Step 2, bottom pad one deck cotton of the culture dish that is 9cm at diameter, water is moistening, chooses thick sword bean blade and is cut into circle, is close to wet cotton, cotton bar is closely trapped among around circular sword bean leaf to moistening processing;
Step 3, the ovum of the new little peaceful mite in the institute California of producing in 30 24h of picking, in circular blade, drops into 1.0g apricot pollen, culture dish is sealed with preservative film to the developmental state of a Predatory Mites of every 12h observation, a pollen of every 24h replacing;
Step 4. picking is hatched in 24h-48h to the ovum on circular blade, experiences and casts off a skin for twice, grows for becoming mite, raise to 6-7d, while having Mating, pairing mating.
Further, in step 1, put under the sword bean leaf of raising box and need to spread paper or the cotton that water absorbing force is strong, often add water and keep paper or cotton moistening; After access Tetranychus cinnabarinus, will note controlling population quantity, can regulate the population of Tetranychus cinnabarinus to change by changing temperature, Tetranychus cinnabarinus be in preference temperature, and the higher growth and breeding of temperature is faster, suitably reduces temperature and can delay growth rate; The access quantity of California genus amblyseius berlese is controlled at a thirtieth left and right of Tetranychus cinnabarinus population quantity, otherwise excessively predation causes Tetranychus cinnabarinus population to decline rapidly, and raising is by unsustainable, if feeding process is found other predators, should kill.
Further, in step 2, bottom pad one deck cotton of the culture dish that is 9cm at diameter, when water is moistening, can not ponding too much, keep moistening; Cotton bar is closely trapped among around circular sword bean leaf, when moistening processing, does not stay gap, but can not make water droplet enter blade.
Further, in step 3, when culture dish sealing with preservative film, need on preservative film, prick aperture and take a breath.
Further, in step 4, further comprising the steps of: the female mite of post-coitum to be put into independent culture dish and raise, each culture dish is put a female mite, observes egg laying amount when every 12h one time, and produced ovum is chosen, put into another culture dish, also can normally hatch, take food growth, and can mate and oviposit.
The method of the new little peaceful mite in employing apricot pollen breeding California provided by the invention, the method of this new little peaceful mite in employing apricot pollen breeding state comprises: choose aging thicker blade, clean, put into and raise box, Tetranychus cinnabarinus, is accessed upward in the back side, in the time that Tetranychus cinnabarinus population reaches 200 left and right, with small size writing brush access California genus amblyseius berlese, with stereomicroscope observation Tetranychus cinnabarinus and California genus amblyseius berlese, grasp population and change; Bottom pad one deck cotton of the culture dish that is 9cm at diameter, water is moistening, chooses thick sword bean blade and is cut into circle, is close to wet cotton, cotton bar is closely trapped among around circular sword bean leaf to moistening processing; The ovum of the new little peaceful mite in the institute California of producing in 30 24h of picking, in circular blade, drops into 1.0g apricot pollen, culture dish is sealed with preservative film to the developmental state of a Predatory Mites of every 12h observation, a pollen of every 24h replacing; Picking is hatched in 24h-48h to the ovum on circular blade, experiences and casts off a skin for twice, grows for becoming mite, raise to 6-7d, and while having Mating, pairing mating; The female mite of post-coitum is put into independent culture dish and raise, each culture dish is put a female mite, observes egg laying amount when every 12h one time, and produced ovum is chosen, and puts into another culture dish, also can normally hatch, and takes food growth, and can mate and oviposit.The Predatory Mites that the present invention adopts pollen to raise needs 6-8d from young mite to adult, egg-laying period two weeks left and right, approximately 1.4 pieces of average daily egg laying amounts, in the suitable situation of condition, life-span can be more than 20d, with the Predatory Mites of raising with natural prey without significant difference, and the ovum producing after the pollen of searching for food, after hatching, still can normally take food Tetranychus cinnabarinus, in the situation that artificial feed are not also broken through at present, raise the new little peaceful mite in California with Apple Pollen and be undoubtedly a feasible new way, practical, there is stronger propagation and employment and be worth.
Accompanying drawing explanation
Fig. 1 is the newly realization flow figure of the method for little peaceful mite of employing apricot pollen breeding California that the embodiment of the present invention provides.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with drawings and Examples, the present invention is described in further detail.Should be appreciated that specific embodiment described herein is only in order to explain the present invention, and be not used in restriction invention.
Fig. 1 shows the newly realization flow of the method for little peaceful mite of employing apricot pollen breeding California that the embodiment of the present invention provides.
The method of this new little peaceful mite in employing apricot pollen breeding California comprises the following steps:
Step S101, chooses aging thicker blade, cleans, and puts into and raises box, upward, access Tetranychus cinnabarinus, in the time that Tetranychus cinnabarinus population reaches 200 left and right at the back side, with small size writing brush access California genus amblyseius berlese, with stereomicroscope observation Tetranychus cinnabarinus and California genus amblyseius berlese, grasp population and change;
Step S102, bottom pad one deck cotton of the culture dish that is 9cm at diameter, water is moistening, chooses thick sword bean blade and is cut into circle, is close to wet cotton, cotton bar is closely trapped among around circular sword bean leaf to moistening processing;
Step S103, the ovum of the new little peaceful mite in the institute California of producing in 30 24h of picking, in circular blade, drops into 1.0g apricot pollen, culture dish is sealed with preservative film to the developmental state of a Predatory Mites of every 12h observation, a pollen of every 24h replacing;
Step S104, picking is hatched in 24h-48h to the ovum on circular blade, experiences and casts off a skin for twice, grows for becoming mite, raise to 6-7d, while having Mating, pairing mating.
In embodiments of the present invention, in step S101, put under the sword bean leaf of raising box and need to spread paper or the cotton that water absorbing force is strong, often add water and keep paper or cotton moistening; After access Tetranychus cinnabarinus, will note controlling population quantity, can regulate the population of Tetranychus cinnabarinus to change by changing temperature, Tetranychus cinnabarinus be in preference temperature, and the higher growth and breeding of temperature is faster, suitably reduces temperature and can delay growth rate; The access quantity of California genus amblyseius berlese is controlled at a thirtieth left and right of Tetranychus cinnabarinus population quantity, otherwise excessively predation causes Tetranychus cinnabarinus population to decline rapidly, and raising is by unsustainable, if feeding process is found other predators, should kill.
In embodiments of the present invention, in step S102, bottom pad one deck cotton of the culture dish that is 9cm at diameter, when water is moistening, can not ponding too much, keep moistening; Cotton bar is closely trapped among around circular sword bean leaf, when moistening processing, does not stay gap, but can not make water droplet enter blade.
In embodiments of the present invention, in step S103, when culture dish sealing with preservative film, need on preservative film, prick aperture and take a breath.
In embodiments of the present invention, in step S104, further comprising the steps of: the female mite of post-coitum to be put into independent culture dish and raise, each culture dish is put a female mite, observes egg laying amount when every 12h one time, and produced ovum is chosen, put into another culture dish, also can normally hatch, take food growth, and can mate and oviposit.
Below in conjunction with drawings and the specific embodiments, application principle of the present invention is further described.
Specific embodiment of the invention process is:
1. the continuity of the population of the new little peaceful mite in California and maintaining
Plantation sword bean, as the food source of the natural prey Tetranychus cinnabarinus of California genus amblyseius berlese, this is because sword bean leaf breeding Tetranychus cinnabarinus is rapid, and sword bean is easy to plantation, simple and convenient management.Choose aging thicker blade, clean, put into and raise box, access Tetranychus cinnabarinus, is prepared against upward in the back side.Under sword bean leaf, need to spread paper or the cotton that water absorbing force is strong, often add water and keep paper or cotton moistening, the one, control acarid is escaped, and the 2nd, maintain the required air humidity of acarid and wet environment, the 3rd, delay the withered of sword bean leaf.With stereomicroscope observation Tetranychus cinnabarinus and California genus amblyseius berlese, grasp its population and change.After access Tetranychus cinnabarinus, to note controlling population quantity, too much can cause sword bean leaf to consume too fast.Can regulate the population of Tetranychus cinnabarinus to change by changing temperature, Tetranychus cinnabarinus be in preference temperature, and the higher growth and breeding of temperature is faster, suitably reduces temperature and can delay its growth rate.In the time that Tetranychus cinnabarinus population reaches some, with small size writing brush access California genus amblyseius berlese, the access quantity of California genus amblyseius berlese can not be too much, otherwise excessively predation causes Tetranychus cinnabarinus population to decline rapidly, and raising is by unsustainable.If feeding process is found other predators as thrips and is killed.
2. the structure of pollen feeding environment
Utensil is the culture dish of diameter 9cm, bottom pad one deck cotton, and water is moistening, but can not ponding too much, keeps moistening.Choose thick sword bean blade and be cut into circle, be close to wet cotton, one can delay blade withers, and two carry out moist environment can be used as the barrier that stops Predatory Mites to be escaped.Again cotton bar is closely trapped among around circular sword bean leaf, moistening, do not stay gap, but can not make water droplet enter blade.
3. pollen is raised and is implemented
The newly ovum of little peaceful mite of the institute California of producing, in circular blade, drops into a small amount of Apple Pollen in picking some 24h, culture dish is sealed with preservative film, pricks several apertures, for taking a breath on preservative film.Every 12h observes the developmental state of a Predatory Mites, and every 24h changes a pollen.
4. observed and recorded
Picking all can be hatched in 24h-48h to the ovum on circular blade.The mite hatching can normally take food pollen, and body colour and pollen are close, experiences and casts off a skin for twice, grows for becoming mite.While raising to 6-7d, there is Mating, matched mating.The female mite of post-coitum is put into independent culture dish and raise, each culture dish is put a female mite.When every 12h, observe once its egg laying amount.Produced ovum is chosen, put into another culture dish and observe, result also can normally be hatched, and takes food growth, and can mate and oviposit.Need 6-8d from young mite to adult, egg-laying period about two weeks, approximately 1.4 pieces of average daily egg laying amounts with the Predatory Mites that pollen is raised.Remove escape, in the suitable situation of condition, the life-span can be more than 20d, and without significant difference, and the ovum producing after the pollen of searching for food, after hatching, still can normally take food Tetranychus cinnabarinus with the Predatory Mites of raising with natural prey.About the degenerate problem of raising some generations of Predatory Mites with pollen, will in experiment afterwards, verify.
The present invention has found pollen that can the new little peaceful mite in successive propagation California, and the new little peaceful mite in indoor propagation California is not vulnerable to environmental constraints.Natural prey is raised the new little peaceful mite in California need to do harm to mite, and the Tetranychus cinnabarinus poor growth in winter that laboratory is used at present, and its host plant winter growing state neither be fine.In the situation that artificial feed are not also broken through at present, raise the new little peaceful mite in California with Apple Pollen and be undoubtedly a feasible new way.
The method of the new little peaceful mite in employing apricot pollen breeding California that the embodiment of the present invention provides, the method of this new little peaceful mite in employing apricot pollen breeding California comprises: choose aging thicker blade, clean, put into and raise box, Tetranychus cinnabarinus, is accessed upward in the back side, in the time that Tetranychus cinnabarinus population reaches some, with small size writing brush access California genus amblyseius berlese, with stereomicroscope observation Tetranychus cinnabarinus and California genus amblyseius berlese, grasp population and change; Bottom pad one deck cotton of the culture dish that is 9cm at diameter, water is moistening, chooses thick sword bean blade and is cut into circle, is close to wet cotton, cotton bar is closely trapped among around circular sword bean leaf to moistening processing; The ovum of the new little peaceful mite in the institute California of producing, in circular blade, drops into a small amount of Apple Pollen in picking some 24h, culture dish is sealed with preservative film to the developmental state of a Predatory Mites of every 12h observation, a pollen of every 24h replacing; Picking is hatched in 24h-48h to the ovum on circular blade, experiences and casts off a skin for twice, grows for becoming mite, raise to 6-7d, and while having Mating, pairing mating; The female mite of post-coitum is put into independent culture dish and raise, each culture dish is put a female mite, observes egg laying amount when every 12h one time, and produced ovum is chosen, and puts into another culture dish, also can normally hatch, and takes food growth, and can mate and oviposit; The Predatory Mites that the present invention adopts pollen to raise needs 6-8d from young mite to adult, egg-laying period two weeks left and right, approximately 1.4 pieces of average daily egg laying amounts, in the suitable situation of condition, life-span can be more than 20d, with the Predatory Mites of raising with natural prey without significant difference, and the ovum producing after the pollen of searching for food, after hatching, still can normally take food Tetranychus cinnabarinus, in the situation that artificial feed are not also broken through at present, raise the new little peaceful mite in California with Apple Pollen and be undoubtedly a feasible new way, practical, there is stronger propagation and employment and be worth.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.

Claims (5)

1. a method that adopts the new little peaceful mite in apricot pollen breeding California, is characterized in that, the method for this new little peaceful mite in employing apricot pollen breeding California comprises the following steps:
Step 1, chooses aging thicker blade, cleans, and puts into and raises box, upward, access Tetranychus cinnabarinus, in the time that Tetranychus cinnabarinus population reaches 200 at the back side, with small size writing brush access California genus amblyseius berlese, with stereomicroscope observation Tetranychus cinnabarinus and California genus amblyseius berlese, grasp population and change;
Step 2, bottom pad one deck cotton of the culture dish that is 9cm at diameter, water is moistening, chooses thick sword bean blade and is cut into circle, is close to wet cotton, cotton bar is closely trapped among around circular sword bean leaf to moistening processing;
Step 3, the ovum of the new little peaceful mite in the institute California of producing in 30 4h of picking, in circular blade, drops into 1.0g apricot pollen, culture dish is sealed with preservative film to the developmental state of a Predatory Mites of every 12h observation, a pollen of every 24h replacing;
Step 4, picking is hatched in 24h-48h to the ovum on circular blade, experiences and casts off a skin for twice, grows for becoming mite, raise to 6-7d, while having Mating, pairing mating.
2. the method for the new little peaceful mite in employing apricot pollen breeding California as claimed in claim 1, its feature, in step 1, is put under the sword bean leaf of raising box and is needed to spread paper or the cotton that water absorbing force is strong, often adds water and keeps paper or cotton moistening; After access Tetranychus cinnabarinus, will note controlling population quantity, can regulate the population of Tetranychus cinnabarinus to change by changing temperature, Tetranychus cinnabarinus be in preference temperature, and the higher growth and breeding of temperature is faster, suitably reduces temperature and can delay growth rate; The access quantity of California genus amblyseius berlese is controlled at below a thirtieth of Tetranychus cinnabarinus population quantity, otherwise excessively predation causes Tetranychus cinnabarinus population to decline rapidly, and raising is by unsustainable, if feeding process is found other predators, should kill.
3. the method for the new little peaceful mite in employing apricot pollen as claimed in claim 1 breeding California, is characterized in that, in step 2, and bottom pad one deck cotton of the culture dish that is 9cm at diameter, when water is moistening, can not ponding too much, keep moistening; Cotton bar is closely trapped among around circular sword bean leaf, when moistening processing, does not stay gap, but can not make water droplet enter blade.
4. the method for the new little peaceful mite in employing apricot pollen breeding California as claimed in claim 1, is characterized in that, in step 3, when culture dish sealing with preservative film, need on preservative film, prick aperture and take a breath.
5. the method for the new little peaceful mite in employing apricot pollen breeding California as claimed in claim 1, it is characterized in that, in step 4, further comprising the steps of: the female mite of post-coitum is put into independent culture dish and raise, each culture dish is put a female mite, when every 12h, observe egg laying amount one time, produced ovum is chosen, put into another culture dish, also can normally hatch, take food growth, and can mate and oviposit.
CN201410117438.8A 2014-03-27 2014-03-27 Method for breeding neoseiulus californicus through apricot blossom pollen Pending CN103858828A (en)

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CN104430194A (en) * 2014-11-14 2015-03-25 中国农业科学院柑桔研究所 Method for artificially feeding euseius nicholsi in pollen room
CN104472437A (en) * 2014-11-20 2015-04-01 中国农业科学院柑桔研究所 Indoor isolation feeder for spider mites
CN106969963A (en) * 2017-04-07 2017-07-21 合肥天敌之家生物技术有限公司 A kind of colouring method of the new small peaceful mite digestive system of Pasteur
CN107466972A (en) * 2017-09-19 2017-12-15 南昌大学 A kind of conservation method for breeding of Tetranychus cinnabarinus
CN108812553A (en) * 2018-05-29 2018-11-16 四川农业大学 A kind of mating system improving small new neoseiulus californicus offspring female ratio
CN112913789A (en) * 2021-04-02 2021-06-08 贵州大学 Predatory mite feeding method taking cattail pollen as feed

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104430194A (en) * 2014-11-14 2015-03-25 中国农业科学院柑桔研究所 Method for artificially feeding euseius nicholsi in pollen room
CN104472437A (en) * 2014-11-20 2015-04-01 中国农业科学院柑桔研究所 Indoor isolation feeder for spider mites
CN106969963A (en) * 2017-04-07 2017-07-21 合肥天敌之家生物技术有限公司 A kind of colouring method of the new small peaceful mite digestive system of Pasteur
CN107466972A (en) * 2017-09-19 2017-12-15 南昌大学 A kind of conservation method for breeding of Tetranychus cinnabarinus
CN108812553A (en) * 2018-05-29 2018-11-16 四川农业大学 A kind of mating system improving small new neoseiulus californicus offspring female ratio
CN112913789A (en) * 2021-04-02 2021-06-08 贵州大学 Predatory mite feeding method taking cattail pollen as feed

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Application publication date: 20140618