CN218025893U

CN218025893U - Malaria protein purification device

Info

Publication number: CN218025893U
Application number: CN202222330356.4U
Authority: CN
Inventors: 何鹏; 卢霞; 王维斌; 王攀; 汪磊; 徐同勋; 生喜印
Original assignee: Xiangpeng Youkang Beijing Technology Co ltd
Current assignee: Xiangpeng Youkang Beijing Technology Co ltd
Priority date: 2022-09-02
Filing date: 2022-09-02
Publication date: 2022-12-13
Anticipated expiration: 2032-09-02

Abstract

The utility model relates to a biological protein technical field, in particular to malaria protein purification device, the power distribution box comprises a box body, vertical the inserting is equipped with a plurality of purification posts in the box, be equipped with temperature control device in the box, wear out the upper end of purification post the box is connected with the multi-ported valve, each the lower extreme of purification post is worn out the box is connected with down the multi-ported valve. Compared with the prior art, the malaria protein purification device provided by the utility model can simultaneously perform temperature control treatment on a plurality of purification columns and perform temperature control treatment on a certain or single purification column, and the malaria protein purification device does not generate influence and interference with each other, thereby meeting more practical application scenes and being practical and flexible; the optimization of the purification column structure can ensure that the liquid is uniformly distributed during protein purification, does not damage the filler layer structure in the egg Bai Chunhua column and has the advantage of good purification effect.

Description

Malaria protein purification device

Technical Field

The utility model relates to a biological protein technical field, in particular to malaria protein purification device.

Background

Circulating Tumor Cells (CTCs) are a very rare class of tumor cells that enter the blood circulation via the vascular or lymphatic system after shedding from a primary tumor during tumor metastasis. Such cells can spread to distant organs through blood circulation and proliferate in a suitable environment, and are the main cause of tumor metastasis in patients. The early detection of CTC in the blood of cancer patients has important significance for judging the survival rate of the patients and evaluating the curative effect. Researchers have found that the malaria protein VAR2CSA binds strongly to a variety of different cancer cells. Therefore, the sensitivity of capturing CTC by VAR2CSA is much higher than that of the traditional immunocapture method, and CTC with various phenotypes can be captured.

At protein expression experiment later stage, need purify fusion protein, generally use the purification post to purify, in the actual operation process, often need a plurality of purification experiment can obtain ideal purification condition, can improve the exploration efficiency and the high flux efficiency of purification condition through increasing purification post quantity. However, when a plurality of purification columns are used for purification, some fusion proteins are sensitive to temperature, the activity of the fusion proteins is often required to be maintained through low temperature, the transfer operation is required in a low-temperature environment, the structural design of the purification columns is unreasonable, and when liquid such as protein sample solution or buffer solution is added, the liquid is concentrated on a certain part of the filler, the surface flatness of the filler is affected, so that the contact between the material and the filler is not uniform, and the purification effect is affected.

SUMMERY OF THE UTILITY MODEL

In order to solve the problems, the utility model provides a malaria protein purification device.

The utility model adopts the technical scheme as follows: a malaria protein purification device, the key lies in: the purifying device comprises a box body, wherein a plurality of purifying columns are vertically inserted into the box body, a temperature control device is arranged in the box body, the upper ends of the purifying columns penetrate out of the box body and are connected with upper multi-way valves, and the lower ends of the purifying columns penetrate out of the box body and are connected with lower multi-way valves.

Preferably, the purification post includes the cylinder, from last to being equipped with branch liquid district, chromatography district and filter down in the cylinder, just about being equipped with two funnels in the branch liquid district, two the little fill mouth of funnel passes through the body and connects, two the outer wall of funnel with the inner wall fixed connection of cylinder, wear to be equipped with the connecting rod in the body, the upper end of this connecting rod is worn out the body is connected with the horizontal stand, horizontal stand and top funnel inner wall fixed connection, the lower extreme of this connecting rod is worn out the body is connected with the toper seat, the conical surface of this toper seat and below form the liquid separating channel between the funnel inner wall.

Preferably, a plurality of barrels vertically penetrate through the box body, the barrels correspond to the purification columns one to one, the inner barrel wall of each barrel is attached to the purification columns, two openings of each barrel are fixedly connected with the top wall and the bottom wall of the box body respectively, a temperature control cavity is formed between the inner wall of the box body and the outer wall of each barrel, and a temperature control liquid inlet pipe and a temperature control liquid outlet pipe are arranged on the box body.

Preferably, the upper end face of the column body is provided with a limiting lug.

Preferably, the device further comprises a controller and a temperature sensor arranged in the box body.

Preferably, the device also comprises a sample container and a buffer solution container, wherein the sample container and the buffer solution container are respectively communicated with the upper multi-way valve pipelines.

Preferably, the device also comprises a product collecting container and a waste liquid collecting container, wherein the product collecting container and the waste liquid collecting container are respectively connected with the lower multi-way valve through pipelines. The product/waste streams from multiple purification columns can be collected together.

Has the advantages that: compared with the prior art, the malaria protein purification device provided by the utility model can simultaneously perform temperature control treatment on a plurality of purification columns and perform temperature control treatment on a certain or single purification column, and no influence and interference are generated among the purification columns, so that more practical application scenes are met, and the device is practical and flexible; the optimization of the purification column structure can ensure that the liquid is uniformly distributed during protein purification, does not damage the filler layer structure in the egg Bai Chunhua column and has the advantage of good purification effect.

Drawings

FIG. 1 is a schematic view of the present invention;

FIG. 2 is a schematic view of the structure of a purification column;

fig. 3 is an enlarged view of fig. 2 at a.

Detailed Description

In order to make the technical solution of the present invention better understood by those skilled in the art, the present invention will be described in detail with reference to the accompanying drawings and the detailed description.

As shown in fig. 1 to 3, a malaria protein purification apparatus comprises a box body 1, wherein a plurality of purification columns 2 are vertically inserted into the box body 1, a temperature control device 3 is arranged in the box body 1, the upper ends of the purification columns 2 penetrate out of the box body 1 and are connected with upper multi-way valves 4, and the lower ends of the purification columns 2 penetrate out of the box body 1 and are connected with lower multi-way valves 5.

In this embodiment, the purification column 2 includes the cylinder 21, from last to being equipped with branch liquid district, chromatography district and filter 23 down in the cylinder 21, just be equipped with two funnels 22 from top to bottom in the branch liquid district, two the little fill mouth of funnel 22 passes through the body 25 and connects, two the outer wall of funnel 22 with the inner wall fixed connection of cylinder 21, wear to be equipped with connecting rod 26 in the body 25, the upper end of this connecting rod 26 is worn out the body 25 is connected with horizontal bracket 27, horizontal bracket and top funnel 22 inner wall fixed connection, the lower extreme of this connecting rod 26 is worn out the body 25 is connected with toper seat 28, this toper seat 28 the conical surface and the below form the separating channel between the funnel 22 inner wall. In the more preferred scheme, the upper end of cylinder is equipped with sealing washer 29, goes up the play liquid end of multi-way valve and inserts the cylinder upper end and pastes tightly with the sealing washer, and sample/buffer solution gets into the cylinder through the feed liquor end of last multi-way valve, and the funnel, the body that flow through the top in proper order again evenly flow into the filler in chromatography district through separating the passageway, and the contact of material and filler is even, and purification effect is good.

In this embodiment, a plurality of barrels 6 vertically run through the box 1, the barrels 6 correspond to the purification columns 2 one by one, the inner cylinder wall of the barrel 6 is attached to the purification columns 2, two cylinder openings of the barrels 6 are fixedly connected to the top wall and the bottom wall of the box 1, a temperature control cavity is formed between the inner wall of the box 1 and the outer wall of each barrel 6, and a temperature control liquid inlet pipe 7 and a temperature control liquid outlet pipe 8 are arranged on the box 1. The temperature control liquid inlet pipe 7 injects low-temperature fluid into the temperature control cavity to meet the temperature requirement of the protein sample in the purification process, in a more preferable scheme, the cylinder body is made of heat-conducting metal materials, so that the heat exchange between the low-temperature fluid and the purification column can be better realized, and in another preferable scheme, the box wall of the box body is covered with heat-insulating materials.

In this embodiment, the upper end surface of the column body 21 is provided with a limiting lug 9. During the in-service use, the cylinder can be convenient insert establish/take out the box, convenient operation.

In this embodiment, the temperature control device further comprises a controller and a temperature sensor arranged in the box body 1. The temperature condition in the box body can be changed to better control the low temperature so as to obtain ideal purification conditions.

In this embodiment, the sample container 10 and the buffer solution container 11 are further included, and the sample container 10 and the buffer solution container 11 are respectively in pipeline communication with each upper multi-way valve 4. Repeated experiments of protein purification can be carried out, the stability of the protein purification scheme is verified, the speed of simultaneously loading a plurality of proteins is reduced, the time for balancing, impurity washing and column regeneration is saved, and the method can also be used for simultaneously purifying a plurality of projects, so that the time for simultaneously purifying a plurality of projects is shortened, and the labor cost is saved.

In this embodiment, the device further comprises a product collecting container 12 and a waste liquid collecting container 13, wherein the product collecting container 12 and the waste liquid collecting container 13 are respectively connected with the lower multi-way valve 5 through pipelines. The product/waste stream from multiple purification columns can be collected together.

Finally, it should be noted that the above description is only a preferred embodiment of the present invention, and that those skilled in the art can make various similar representations without departing from the spirit and scope of the present invention.

Claims

1. A malaria protein purification apparatus, characterized in that: the purifying device comprises a box body (1), wherein a plurality of purifying columns (2) are vertically inserted into the box body (1), a temperature control device (3) is arranged in the box body (1), the upper ends of the purifying columns (2) penetrate out of the box body (1) and are connected with upper multi-way valves (4), and the lower ends of the purifying columns (2) penetrate out of the box body (1) and are connected with lower multi-way valves (5);

purification post (2) includes cylinder (21), be equipped with branch liquid district, chromatography district (24) and filter (23) from last to down in cylinder (21), just be equipped with two funnel (22) about in the branch liquid district, two the little fill mouth of funnel (22) passes through body (25) and connects, two the outer wall of funnel (22) with the inner wall fixed connection of cylinder (21), wear to be equipped with connecting rod (26) in body (25), the upper end of this connecting rod (26) is worn out body (25) is connected with horizontal stand (27), horizontal stand and top funnel (22) inner wall fixed connection, the lower extreme of this connecting rod (26) is worn out body (25) are connected with toper seat (28), the conical surface and the below of this toper seat (28) form separating channel (a) between funnel (22) the inner wall.

2. A malaria protein purification apparatus according to claim 1, wherein: the device is characterized in that a plurality of barrels (6) are vertically arranged in the box body (1) in a penetrating mode, the barrels (6) correspond to the purification columns (2) one by one, the inner barrel wall of each barrel (6) is attached to the purification columns (2), two barrel openings of each barrel (6) are fixedly connected with the top wall and the bottom wall of the box body (1), a temperature control cavity (b) is formed between the inner wall of the box body (1) and the outer wall of each barrel (6), and a temperature control liquid inlet pipe (7) and a temperature control liquid outlet pipe (8) are arranged on the box body (1).

3. A malaria protein purification apparatus according to claim 2, wherein: the upper end face of the column body (21) is provided with a limiting lug (9).

4. A malaria protein purification apparatus according to claim 1, wherein: the temperature control device (3) comprises a controller and a temperature sensor arranged in the box body (1).

5. A malaria protein purification apparatus according to claim 1, wherein: the device is characterized by further comprising a sample container (10) and a buffer solution container (11), wherein the sample container (10) and the buffer solution container (11) are respectively communicated with the upper multi-way valves (4) through pipelines.

6. A malaria protein purification apparatus according to claim 1, wherein: the device is characterized by further comprising a product collecting container (12) and a waste liquid collecting container (13), wherein the product collecting container (12) and the waste liquid collecting container (13) are respectively in pipeline connection with the lower multi-way valves (5).