CN202201904U - Dissolved oxygen control system in high cell density fermentation process - Google Patents
Dissolved oxygen control system in high cell density fermentation process Download PDFInfo
- Publication number
- CN202201904U CN202201904U CN 201120351090 CN201120351090U CN202201904U CN 202201904 U CN202201904 U CN 202201904U CN 201120351090 CN201120351090 CN 201120351090 CN 201120351090 U CN201120351090 U CN 201120351090U CN 202201904 U CN202201904 U CN 202201904U
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- CN
- China
- Prior art keywords
- dissolved oxygen
- fermented liquid
- sterile air
- sparger
- cell density
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn - After Issue
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a dissolved oxygen control system in high cell density fermentation process, which well solves the problem of greatly regulating the dissolved oxygen in the high cell density fermentation process. A sterile air distributor is arranged between the inlet and the outlet of a fermentation tank, and a fermentation broth circulating pump, a fermentation hydraulic force regulating control valve in the distributor and the like are arranged between the fermentation tank and the sterile air distributor. In the dissolved oxygen control system, the dissolved oxygen concentration can be greatly improved, the dissolved oxygen can be regulated in a wide range, the pollution of the fermentation broth to an air distributor membrane can be reduced, the energy consumption is saved, the overall requirements on the equipment are reduced, and the investment is reduced, so that the dissolved oxygen control system has good application prospect.
Description
Technical field:
The utility model relates to a kind of zymotechnique, relates to The Control of Dissolved Oxygen system in a kind of fermenting process or rather.
Background technology:
During the fermentation, improve microorganism cells density, can significantly improve the production efficiency of unit volume container; Reduce the separation costs of living weight, also can shorten the production cycle, minimizing equipment input, thereby reduce production costs; Enhance productivity; Therefore, being engaged in high cell density fermentation, is one of the field of fermenting now emerging technology with the fastest developing speed.Metabolic activity for most mikrobes; All need take in oxygen, discharge carbonic acid gas, when mikrobe breathes; Can only utilize the oxygen molecule that is dissolved in the nutrient solution; Oxygen molecule gets into cell through cytolemma and could be utilized by mikrobe, and therefore, dissolved oxygen concentration is that microbial fermentation especially influences one of thalli growth and metabolic important factor in the high cell density fermentation process.Mikrobe is at different growth phases, and is different to the demand of dissolved oxygen, and the concentration control of suitable dissolved oxygen plays an important role to fermenting process; Especially in the fermentation later stage, because cell density sharply increases, oxygen-consumption is very big, and can requirement that satisfy dissolved oxygen often become the successful limiting factor of fermenting.For high cell density fermentation, make to keep suitable dissolved oxygen concentration in the fermenting process, fermentation equipment must have the adjusting The Control of Dissolved Oxygen ability of wide range.
Traditional dissolved oxygen is regulated; Normal adopt measures such as regulating in air flow, stirring velocity, the raising fermentor tank pressures, ferment, under the less situation of oxygen requirement for routine; Can also meet the demands; But to high density fermentation, when the oxygen requirement peak was very big, just solve very difficulty by the way: 1, air flow was big, the high energy consumption that causes of rotating speed is big; 2, air flow is big, air velocity is high, and oxygen utilization rate is low, should form foam in jar, influences equipment utilization; 3, the too high pair cell of stirring velocity is prone to damage, and intracellular organic matter discharges influences the stable of meta-bolites; 4, the equipment integral design requirements high, cause facility investment big; 5, surpass certain cell density, can't realize toward contact.
Summary of the invention:
The purpose of the utility model is that the deficiency that overcomes prior art provides dissolved oxygen system in a kind of high cell density fermentation process, can significantly improve oxyty.
The utility model is characterised in that between import at fermentor tank, the outlet sterile air sparger is set; Between fermentor tank outlet and sterile air sparger, fermented liquid recycle pump, sterile air sparger air valve are set; Fermented liquid pressure adjusting control valve in the sparger is set between fermentor tank import and sterile air sparger, and the import of sterile air sparger is provided with sterile air sparger inlet mouth tensimeter, fermented liquid main road valve; Between the fermented liquid pressure adjusting control valve fermented liquid bypass control valve, fermented liquid circulating-pump outlet tensimeter are set in fermented liquid recycle pump and the sparger.
Can know that by technique scheme the utility model proposes to improve design to having fermentation equipment now, and fermented liquid is derived outside the jar; On fermented liquid derivation branch road, utilize the dissolved oxygen system that the fermented liquid oxyty is regulated, the sparger film is cleaned, can significantly improve oxyty, realize the adjusting of dissolved oxygen wide region; Save energy consumption simultaneously, reduce the whole requirement of equipment, reduce investment; Have good application prospects, be of great immediate significance.
Description of drawings:
Accompanying drawing is the structural representation of the utility model.
Among the figure, 1. fermentor tank, 2. sterile air sparger; 3. fermented liquid recycle pump, 4. fermented liquid bypass control valve, 5. fermented liquid circulating-pump outlet tensimeter; 6. sterile air sparger air valve; 7. fermented liquid pressure adjusting control valve, 8. sterile air sparger inlet mouth tensimeter, 9. fermented liquid main road valve in the sparger.
Embodiment:
Below in conjunction with accompanying drawing the utility model is further described.
Shown in accompanying drawing; Between the import of fermentor tank 1, outlet, sterile air sparger 2 is set; Between fermentor tank 1 outlet and sterile air sparger 2, fermented liquid recycle pump 3, sterile air sparger air valve 6 are set; Fermented liquid pressure adjusting control valve 7 in the sparger is set between fermentor tank 1 import and sterile air sparger 2, and 2 imports of sterile air sparger are provided with sterile air sparger inlet mouth tensimeter 8, fermented liquid main road valve 9; Between the fermented liquid pressure adjusting control valve 7 fermented liquid bypass control valve 4, fermented liquid circulating-pump outlet tensimeter 5 are set in fermented liquid recycle pump 3 and the sparger.
The sterile air sparger 2 of the utility model utilizes wide aperture tubular type hyperfiltration membrane assembly to change system into; The sterile air dividing potential drop is high, atomizing is thin; Dissolved oxygen concentration can be with dividing potential drop, flow rate regulation, and blowing air, stirring significantly improve in the more conventional fermentor tank of dissolved oxygen peak concentration value; Adopt the transformation of wide aperture tubular type ultra-filtration membrane, fermented liquid can the plug assembly fermentation broth stream not threaded a pipe; As long as adjust fermented liquid circulating-pump outlet tensimeter, the manometric force value of sterile air sparger inlet mouth, guarantee that the latter greater than the former, has stoped fermented liquid to pass ultra-filtration membrane, can not form fenestra obstruction and pollution.
Offer fermented liquid circulation bypass on sterile air sparger 2 sides; When inactive sterile air sparger 2,, close fermented liquid main road valve 9 then as long as open fermented liquid bypass control valve 4; Let sterile air that the fermented liquid of sterile air sparger 2 is cleaned out; Close fermented liquid pressure adjusting control in the sparger again, 7, close the process that the sterile air valve is accomplished the cleaning sparger at once, stopped using sparger then.
Claims (1)
1. dissolved oxygen system in the high cell density fermentation process; It is characterized in that in fermentor tank (1) import, the outlet between sterile air sparger (2) is set; Between fermentor tank (1) outlet and sterile air sparger (2), fermented liquid recycle pump (3), sterile air sparger air valve (6) are set; Fermented liquid pressure adjusting control valve (7) in the sparger is set between fermentor tank (1) import and sterile air sparger (2), and sterile air sparger (2) import is provided with sterile air sparger inlet mouth tensimeter (8), fermented liquid main road valve (9); Between the fermented liquid pressure adjusting control valve (7) fermented liquid bypass control valve (4), fermented liquid circulating-pump outlet tensimeter (5) are set in fermented liquid recycle pump (3) and the sparger.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120351090 CN202201904U (en) | 2011-09-18 | 2011-09-18 | Dissolved oxygen control system in high cell density fermentation process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120351090 CN202201904U (en) | 2011-09-18 | 2011-09-18 | Dissolved oxygen control system in high cell density fermentation process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202201904U true CN202201904U (en) | 2012-04-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120351090 Withdrawn - After Issue CN202201904U (en) | 2011-09-18 | 2011-09-18 | Dissolved oxygen control system in high cell density fermentation process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202201904U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102399690A (en) * | 2011-09-18 | 2012-04-04 | 淮北市三和诺生物工程有限责任公司 | Oxygen dissolution control system during high cell density fermentation process |
-
2011
- 2011-09-18 CN CN 201120351090 patent/CN202201904U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102399690A (en) * | 2011-09-18 | 2012-04-04 | 淮北市三和诺生物工程有限责任公司 | Oxygen dissolution control system during high cell density fermentation process |
| CN102399690B (en) * | 2011-09-18 | 2013-01-09 | 淮北市三和诺生物工程有限责任公司 | Oxygen dissolution control system during high cell density fermentation process |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20120425 Effective date of abandoning: 20130306 |
|
| RGAV | Abandon patent right to avoid regrant |