CN204999919U - Chip and cell magnetism sorting unit are selected separately to cell magnetism - Google Patents
Chip and cell magnetism sorting unit are selected separately to cell magnetism Download PDFInfo
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- CN204999919U CN204999919U CN201520669791.7U CN201520669791U CN204999919U CN 204999919 U CN204999919 U CN 204999919U CN 201520669791 U CN201520669791 U CN 201520669791U CN 204999919 U CN204999919 U CN 204999919U
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Abstract
The utility model discloses a chip and cell magnetism sorting unit are selected separately to cell magnetism selects separately the chip and includes the liquid pipeline, the liquid pipeline is the shape that gradually bursts at the seams, the liquid pipeline includes first port, second port, third port and fourth port, the third port is located what gradually burst at the seams is the most inner, the fourth port is located the outer end of gradually bursting at the seams, first port and second port all are in between third port and the fourth port, just first port more is close to than second port the third port, the second port is used for connecting waits to select separately cell solution, first port is used for connecting the flush fluid, the third port is used for collecting the target cell, the fourth port is used for collecting remains liquid. The utility model discloses simple structure, it is high -efficient reliable, be fit for continuous, large capacity magnetism cell separation, the integrated target cell washing function of device realizes selecting separately, washing and once accomplish, can realize high -quality continuous cell sorting.
Description
[technical field]
The utility model relates to a kind of Cell magnetic sorting chip and Cell magnetic sorting unit.
[background technology]
Magneticsubstance can be combined with target cell specificity by biotechnology, the magnetic of the target cell utilizing magnetic to mark, sub-electing the target cell (target cell) of high degree of specificity with electromagnetic method, is the final steps of cell medical treatment and cell scientific research activity.Existing cell sorting scheme and cell sorting device also exist that operation is more complicated, the efficiency of separation is low, the not high drawback of separating purity.
[summary of the invention]
In order to overcome the deficiencies in the prior art, the utility model provides the Cell magnetic sorting chip and Cell magnetic sorting unit that a kind of structure is more simple, effect is better.
A kind of Cell magnetic sorting chip, comprise liquid line, described liquid line is the shape of involute urve, described liquid line comprises the first port, second port, 3rd port and the 4th port, described 3rd port is positioned at the inner terminal of described involute urve, described 4th port is positioned at the outermost end of described involute urve, described first port and the second port are all between described 3rd port and the 4th port, and described first port than the second port closer to described 3rd port, described second port is used for connection and treats sorting cells solution, described first port is for connecting washing fluid, described 3rd port is for collecting target cell, described 4th port is for collecting residual liquid.
The utility model additionally provides a kind of Cell magnetic sorting unit, comprise described Cell magnetic sorting chip, also comprise rotating magnetic field generator, described rotating magnetic field generator is used for applying the direction of motion magnetic field contrary with the expansion direction of described involute urve to described Cell magnetic sorting chip.
In one embodiment, the speed of sorting cells solution for the treatment of injected from described second port is less than the washing fluid injected from described first port and flows to the speed of the 4th port, described in treat that sorting cells solution contains Magnetic labeled cells and do not use Magnetic labeled cells.
In one embodiment, also comprise and treat sorting cells source of solvent, washing fluid source, cell solution constant current syringe pump system, washing fluid constant current syringe pump system, the entrance of described cell solution constant current syringe pump system is treated that sorting cells source of solvent is connected and exports be connected with described second port with described, and the entrance of described washing fluid constant current syringe pump system is connected with described washing fluid source and exports and is connected with described first port.
In one embodiment, described cell solution constant current syringe pump system comprises: cell solution first ram pump, cell solution second ram pump, cell solution first check valve, cell solution second check valve, cell solution the 3rd check valve, cell solution the 4th check valve, cell solution drain pipe and cell solution liquid-inlet pipe, described cell solution first plunger pump outlet is connected with the entrance of cell solution first check valve and the outlet of cell solution second check valve respectively, the outlet of described cell solution first check valve is connected with described cell solution drain pipe, described cell solution liquid-inlet pipe is connected with the entrance of described cell solution second check valve and the entrance of described cell solution the 3rd check valve respectively, described cell solution second plunger pump outlet is connected with the outlet of described cell solution the 3rd check valve and the entrance of cell solution the 4th check valve respectively, the outlet of described cell solution the 4th check valve is connected with described cell solution drain pipe.
In one embodiment, in setting-up time section, the liquid velocity sum that the liquid velocity of outlet outflow of described cell solution first ram pump and the outlet of described cell solution second ram pump are flowed out is the first setting speed.
In one embodiment, within certain for some time of described setting-up time section, the liquid velocity that the outlet of described cell solution first ram pump is flowed out linearly reduces, and the liquid velocity that the outlet of described cell solution second ram pump is flowed out linearly increases.
In one embodiment, comprise washing fluid first ram pump, washing fluid second ram pump, washing fluid first check valve, washing fluid second check valve, washing fluid the 3rd check valve, washing fluid the 4th check valve, washing fluid drain pipe and washing fluid liquid-inlet pipe, described washing fluid first plunger pump outlet is connected with the entrance of washing fluid first check valve and the outlet of washing fluid second check valve respectively, the outlet of described washing fluid first check valve is connected with described washing fluid drain pipe, described washing fluid liquid-inlet pipe is connected with the entrance of described washing fluid second check valve and the entrance of described washing fluid the 3rd check valve respectively, described washing fluid second plunger pump outlet is connected with the outlet of described washing fluid the 3rd check valve and the entrance of washing fluid the 4th check valve respectively, the outlet of described washing fluid the 4th check valve is connected with described washing fluid drain pipe.
In one embodiment, in setting-up time section, the liquid velocity sum that the liquid velocity of outlet outflow of described washing fluid first ram pump and the outlet of described washing fluid second ram pump are flowed out is the first setting speed.
In one embodiment, within certain for some time of described setting-up time section, the liquid velocity that the outlet of described washing fluid first ram pump is flowed out linearly reduces, and the liquid velocity that the outlet of described washing fluid second ram pump is flowed out linearly increases.
The beneficial effects of the utility model are:
The utility model structure is simple, high efficient and reliable, is applicable to continuous, Large Copacity Magnetic cell sorting; The integrated target cell pre-washing function of device, realizes sorting, flushing once completes, can realize high-quality successive cell sorting.
The utility model sorting is in airtight pipeline to be carried out, cell solution drive unit, washing fluid drive unit, magnetic separation chip, connecting pipeline, associated vessel can be all single use design, environmental factors can be avoided the pollution of operating process, high quantity and highly purified sorting cells product can be obtained, be conducive to other cell product of sorting clinical grade.
[accompanying drawing explanation]
Fig. 1 is the structural representation of the Cell magnetic sorting unit of a kind of embodiment of the utility model;
Fig. 2 is the structural representation of the Cell magnetic sorting chip in Fig. 1;
Fig. 3 is the structural representation of the rotating magnetic field generator in Fig. 1;
Fig. 4 is the assembling schematic diagram of Fig. 2 and Fig. 3;
Fig. 5 is the structural representation of the Cell magnetic sorting chip of Fig. 2;
Fig. 6 is the equivalent structure schematic diagram of the Cell magnetic sorting chip of Fig. 5;
Fig. 7 is the structural representation of the constant current syringe pump system of Fig. 1;
Fig. 8 is the flow velocity schematic diagram of the constant current syringe pump system of Fig. 7.
[embodiment]
Below the preferred embodiment of utility model is described in further detail.
As shown in Fig. 1 to 8, a kind of Cell magnetic sorting unit, comprises and treats sorting cells source of solvent 103, cell solution constant current syringe pump system 106, Cell magnetic sorting chip 101, rotating magnetic field generator (end face rotating magnetic field generator) 112, washing fluid constant current syringe pump system 111, washing fluid source 108, residual liquid collection container 104, target cell collection container 109.
Cell solution constant current syringe pump system 106 for, drive the cell solution of target cell (target magnetic cell) containing magnetic mark treating sorting to enter Cell magnetic sorting chip 101; Washing fluid constant current syringe pump system 111 for, drive washing fluid to enter Cell magnetic sorting chip 101, make washing fluid rinse target magnetic cell, remove the cell without magnetic mark; Cell magnetic sorting chip 101 for, under magnetic field and the magnetic action power of magnetic cell and the hydrodynamic force of washing fluid, target magnetic cell is sorted and rinses; Rotating magnetic field generator 112 for, produce rotatingfield and drag target magnetic cell target approach cell collection container 109 in Cell magnetic sorting chip 101.
Cell solution constant current syringe pump system 106 can comprise: the first ram pump 11, second ram pump 14, first check valve 16, second check valve 17, 3rd check valve 18, 4th check valve 19, drain pipe 20 and liquid-inlet pipe 21, the outlet 12 of described first ram pump 11 is connected with the entrance of the first check valve 16 and the outlet of the second check valve 17 respectively, the outlet of described first check valve 16 is connected with described drain pipe 20, described liquid-inlet pipe 21 is connected with the entrance of described second check valve 17 and the entrance of described 3rd check valve 18 respectively, the outlet 15 of described second ram pump 14 is connected with the outlet of described 3rd check valve 18 and the entrance of the 4th check valve 19 respectively, the outlet of described 4th check valve 19 is connected with described drain pipe 20.In cell solution constant current syringe pump system 106, liquid-inlet pipe 21 is pipeline 105, and drain pipe 20 is pipeline 102.
As shown in Figure 7, when the first plunger 10 moves right the liquid extruded in it, the outlet pump of the first ram pump 11 goes out liquid, and the first plunger 10 is to the outlet influent (imbitition) of the first ram pump 11 during left movement.The principle of work of the second plunger 13 is identical.In one embodiment, in setting-up time section (such as within the S2 time period), the liquid velocity sum that the liquid velocity of outlet outflow of described first ram pump 11 and the outlet of described second ram pump 14 are flowed out is the first setting speed, thus the speed that ensure that the liquid that drain pipe 20 flows out is constant speed.
In one embodiment, within certain for some time of described setting-up time section, the liquid velocity that the outlet of described first ram pump 11 is flowed out linearly reduces, and the liquid velocity that the outlet of described second ram pump 14 is flowed out linearly increases, the same like this speed that can ensure the liquid that drain pipe 20 flows out is constant speed, and the speed of linear change easily realizes.
In one embodiment, within certain for some time of described setting-up time section, the liquid velocity that the outlet of described first ram pump 11 is flowed out linearly increases, and the liquid velocity that the outlet of described second ram pump 14 is flowed out linearly reduces, thus, first ram pump 11 and the second ram pump 14 alternately absorption, injecting fluid, makes flow rate of liquid constant current and flow velocity is adjustable.
In one embodiment, within certain for some time of described setting-up time section, the liquid velocity that the outlet of described first ram pump 11 is flowed out linearly is increased to described first setting speed, then maintains described setting speed, then linearly reduces.
In one embodiment, within certain for some time of described setting-up time section, the liquid velocity that the outlet of described second ram pump 14 is flowed out linearly is increased to described first setting speed, then maintains described setting speed, then linearly reduces.
In one embodiment, after the liquid velocity that the outlet of described first ram pump 11 is flowed out linearly is decreased to zero, liquid flows into described first ram pump 11 with the second setting speed from the outlet of described first ram pump, thus realizes the first ram pump 11 imbitition.
In one embodiment, after the liquid velocity that the outlet of described second ram pump 14 is flowed out linearly is decreased to zero, liquid flows into described second ram pump 14 with the second setting speed from the outlet of described second ram pump.
Washing fluid constant current syringe pump system 111 can adopt identical pump system, and when Fig. 7 is washing fluid constant current syringe pump system 111, liquid-inlet pipe 21 is pipeline 110, and drain pipe 20 is pipeline 107.
As Fig. 1, 2, shown in 5 and 6, a kind of Cell magnetic sorting chip 101 of embodiment, comprise liquid line 1011, described liquid line is the shape of involute urve, described liquid line 1011 comprises the first port one, second port 2, 3rd port 3 and the 4th port 4, described 3rd port 3 is positioned at the inner terminal of described involute urve, described 4th port 4 is positioned at the outermost end of described involute urve, described first port one and the second port 2 are all between described 3rd port 3 and the 4th port 4, and described first port one than the second port 2 closer to described 3rd port 3, described second port 2 is connected by pipeline 105 treats sorting cells source of solvent 103, described first port one connects washing fluid source 108 by pipeline 110, described 3rd port 3 linking objective cell collection container 109, described 4th port 4 connects residual liquid collection container 104.
As shown in Fig. 1,5 and 6, wherein Fig. 6 is the equivalent schematic diagram of Fig. 5, and in Fig. 5 and 6, black circle represents Magnetic labeled cells, and soft dot represents does not use Magnetic labeled cells.First port one connects washing fluid source 108, forms two articles of streams: the first port one is to the washing fluid stream of the 4th port 4, and the first port one is to the stream of the 3rd port 3; Second port 2 injects treats sorting cells solution, and treats that sorting cells flow velocity is less than the washing fluid flow velocity of the first port one to the 4th port 4.
When the sense of rotation of the rotatingfield that rotating magnetic field generator 112 produces is contrary with involute urve expansion direction, when this rotatingfield puts on involute urve liquid line 1011, magnetic field meeting drag target magnetic cell ecto-entad is (from the 4th port 4 to the three port 3 direction, inverse washing fluid flow velocity direction) motion, prevent target magnetic cell to run off via the 4th port 4, and realize target magnetic cell rinse; When target magnetic cell under rotatingfield drags after the first port one, magnetic cell is collected into target cell collection container 109 by the first port one to the washing fluid of the 3rd port 3, completes cell sorting flow process.
Above content is in conjunction with concrete preferred implementation further detailed description of the utility model, can not assert that concrete enforcement of the present utility model is confined to these explanations.For the utility model person of an ordinary skill in the technical field; without departing from the concept of the premise utility; some simple deduction or replace can also be made, all should be considered as belonging to the scope of patent protection that the utility model is determined by submitted to claims.
Claims (10)
1. a Cell magnetic sorting chip, it is characterized in that, comprise liquid line, described liquid line is the shape of involute urve, described liquid line comprises the first port, second port, 3rd port and the 4th port, described 3rd port is positioned at the inner terminal of described involute urve, described 4th port is positioned at the outermost end of described involute urve, described first port and the second port are all between described 3rd port and the 4th port, and described first port than the second port closer to described 3rd port, described second port is used for connection and treats sorting cells solution, described first port is for connecting washing fluid, described 3rd port is for collecting target cell, described 4th port is for collecting residual liquid.
2. a Cell magnetic sorting unit, it is characterized in that, comprise Cell magnetic sorting chip as claimed in claim 1, also comprise rotating magnetic field generator, described rotating magnetic field generator is used for applying the direction of motion magnetic field contrary with the expansion direction of described involute urve to described Cell magnetic sorting chip.
3. Cell magnetic sorting unit as claimed in claim 2, it is characterized in that, the speed of sorting cells solution for the treatment of injected from described second port is less than the washing fluid injected from described first port and flows to the speed of the 4th port, described in treat that sorting cells solution contains Magnetic labeled cells and do not use Magnetic labeled cells.
4. Cell magnetic sorting unit as claimed in claim 2, it is characterized in that, also comprise and treat sorting cells source of solvent, washing fluid source, cell solution constant current syringe pump system, washing fluid constant current syringe pump system, the entrance of described cell solution constant current syringe pump system is treated that sorting cells source of solvent is connected and exports be connected with described second port with described, and the entrance of described washing fluid constant current syringe pump system is connected with described washing fluid source and exports and is connected with described first port.
5. Cell magnetic sorting unit as claimed in claim 4, it is characterized in that, described cell solution constant current syringe pump system comprises: cell solution first ram pump, cell solution second ram pump, cell solution first check valve, cell solution second check valve, cell solution the 3rd check valve, cell solution the 4th check valve, cell solution drain pipe and cell solution liquid-inlet pipe, described cell solution first plunger pump outlet is connected with the entrance of cell solution first check valve and the outlet of cell solution second check valve respectively, the outlet of described cell solution first check valve is connected with described cell solution drain pipe, described cell solution liquid-inlet pipe is connected with the entrance of described cell solution second check valve and the entrance of described cell solution the 3rd check valve respectively, described cell solution second plunger pump outlet is connected with the outlet of described cell solution the 3rd check valve and the entrance of cell solution the 4th check valve respectively, the outlet of described cell solution the 4th check valve is connected with described cell solution drain pipe.
6. Cell magnetic sorting unit as claimed in claim 5, is characterized in that, the liquid velocity sum that the liquid velocity of outlet outflow of described cell solution first ram pump and the outlet of described cell solution second ram pump are flowed out is the first setting speed.
7. Cell magnetic sorting unit as claimed in claim 5, is characterized in that, the liquid velocity that the outlet of described cell solution first ram pump is flowed out linearly reduces, and the liquid velocity that the outlet of described cell solution second ram pump is flowed out linearly increases.
8. Cell magnetic sorting unit as claimed in claim 4, it is characterized in that, comprise washing fluid first ram pump, washing fluid second ram pump, washing fluid first check valve, washing fluid second check valve, washing fluid the 3rd check valve, washing fluid the 4th check valve, washing fluid drain pipe and washing fluid liquid-inlet pipe, described washing fluid first plunger pump outlet is connected with the entrance of washing fluid first check valve and the outlet of washing fluid second check valve respectively, the outlet of described washing fluid first check valve is connected with described washing fluid drain pipe, described washing fluid liquid-inlet pipe is connected with the entrance of described washing fluid second check valve and the entrance of described washing fluid the 3rd check valve respectively, described washing fluid second plunger pump outlet is connected with the outlet of described washing fluid the 3rd check valve and the entrance of washing fluid the 4th check valve respectively, the outlet of described washing fluid the 4th check valve is connected with described washing fluid drain pipe.
9. Cell magnetic sorting unit as claimed in claim 8, is characterized in that, the liquid velocity sum that the liquid velocity of outlet outflow of described washing fluid first ram pump and the outlet of described washing fluid second ram pump are flowed out is the first setting speed.
10. Cell magnetic sorting unit as claimed in claim 8, is characterized in that, the liquid velocity that the outlet of described washing fluid first ram pump is flowed out linearly reduces, and the liquid velocity that the outlet of described washing fluid second ram pump is flowed out linearly increases.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520669791.7U CN204999919U (en) | 2015-08-31 | 2015-08-31 | Chip and cell magnetism sorting unit are selected separately to cell magnetism |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520669791.7U CN204999919U (en) | 2015-08-31 | 2015-08-31 | Chip and cell magnetism sorting unit are selected separately to cell magnetism |
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| Publication Number | Publication Date |
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| CN204999919U true CN204999919U (en) | 2016-01-27 |
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| CN201520669791.7U Withdrawn - After Issue CN204999919U (en) | 2015-08-31 | 2015-08-31 | Chip and cell magnetism sorting unit are selected separately to cell magnetism |
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| CN (1) | CN204999919U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105062887A (en) * | 2015-08-31 | 2015-11-18 | 张利峰 | Cell magnetic separation chip and cell magnetic separation device |
-
2015
- 2015-08-31 CN CN201520669791.7U patent/CN204999919U/en not_active Withdrawn - After Issue
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105062887A (en) * | 2015-08-31 | 2015-11-18 | 张利峰 | Cell magnetic separation chip and cell magnetic separation device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160601 Address after: Shenzhen City, Baoan District province Guangdong District Longchang road 518000 67 Daqian Industrial Park Building 3, 1 floor, room 03 Patentee after: SHENZHEN SAITELUO BIOLOGY MEDICAL TECHNOLOGY CO., LTD. Address before: Shenzhen City, Baoan District province Guangdong District Longchang road 518000 67 Daqian Industrial Park Building 3, 1 floor, room 03 Patentee before: Zhang Lifeng Patentee before: Wan Shubo Patentee before: Wang Hua |
|
| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20160127 Effective date of abandoning: 20180213 |