CN206375924U - Nucleic acid purification magnetic frame - Google Patents
Nucleic acid purification magnetic frame Download PDFInfo
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- CN206375924U CN206375924U CN201621406454.XU CN201621406454U CN206375924U CN 206375924 U CN206375924 U CN 206375924U CN 201621406454 U CN201621406454 U CN 201621406454U CN 206375924 U CN206375924 U CN 206375924U
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- magnetic
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Abstract
The utility model discloses a kind of nucleic acid purification magnetic frame, the magnetic frame is used to carry out magnetic to magnetisable material in test tube, the magnetic frame includes magnet frame and the rack for test tube being removably mounted to above magnet frame, magnet frame is provided with the magnetic force post matrix that uniform vertical is fixed on magnetic force post installing plate, and magnetic force post is the cylinder-shaped magnet with cavity, the cuvette cavity bottom of holding test tubes is just inserted in the cavity of cylinder-shaped magnet, test tube lower end is surrounded by magnetic force post, therefore can comprehensive offer magnetic force, so that the magnetic force of magnetic is greatly enhanced, absorption affinity to magnetic bead does not decay with enrichment with magnetic bead, so that magnetic bead has stronger adhesive force, so as to strengthen the adaptability of magnetic bead collection, greatly improve nucleic acid extraction efficiency, avoid magnetic force post magnetic too small and cause magnetic bead to be reunited, the magnetic time is long, the halfway phenomenon of magnetic.
Description
Technical field
The utility model belongs to biotinylated nucleic acid technical field of purification, more particularly to a kind of paramagnetic particle method nucleic acid purification magnetic force
Frame.
Background technology
The principle that paramagnetic particle method extracts nucleic acid be with nanometer technology the surface of superparamagnetic nano particle is carried out improvement and
After surface modification, superparamagnetism silica nanometer magnetic bead is prepared into.The magnetic bead can be specific with nucleic acid molecules on micro interface
Ground is recognized and efficiently combination.In the presence of the salt such as guanidine hydrochloride, guanidinium isothiocyanate and externally-applied magnetic field, super-paramagnetism nano magnetic bead energy
Nucleic acid is separated from blood, animal tissue, food, pathogenic microorganism equal samples.This method is widely used in clinical disease
During sick diagnosis, Forensic Identification, environmental microorganism detection and molecular biology research etc. are multi-field.
Magnetic separation technique is that the enrichment with magnetic bead absorption on magnetic-particle surface will be specifically bound under magnetic fields at certain
One position, makes magnetic bead be separated with other materials, reaches the purpose of purifying.The separation method of current magnetic bead and other materials is mainly
Reaction tube is positioned on magnetic frame, the fixed position of magnetic frame is provided with bar magnet, by the magnetic force of bar magnet by magnetic bead in reaction tube
Separated with namagnetic substance.Generally there are two class magnetic frames, a class is that bar magnet is located between two row's reaction tubes, is provided for reaction tube
The magnetic force of one side carries out magnetic bead absorption, and it is not enough that this common magnetic frame has magnetic force, a small amount of magnetic bead sink to ttom of pipe not by
The problem of absorption；Another kind of to be, magnetic bar is located at below test tube, and the acting surface of magnetic bar is often the plane on top, its magnetic field
Active area is big, and magnetic field intensity is weak, causes magnetic bead not concentrated on centrifugation tube wall after adsorbing, in larger bulk, easily causes magnetic
Pearl loses influence nucleic acid (DNA/RNA) yield, and expensive.
To overcome disadvantage mentioned above, the design people is subject to positive innovation research, to create a kind of new nucleic acid purification
Use magnetic frame.
Utility model content
The utility model is strong and right with magnetic mainly solving the technical problems that provide a kind of nucleic acid purification magnetic frame
The big advantage of magnetic bead adsorption area, and then nucleic acid extraction rate is high, residual is few；And once can extract multiple samples, efficiency high.
In order to solve the above technical problems, the further technical scheme that the utility model is used is：A kind of nucleic acid purification is provided
With magnetic frame, including magnet frame and the rack for test tube that is removably mounted to above magnet frame, the magnet frame include magnet frame base,
The magnetic force post matrix that the magnetic force post installing plate and uniform vertical being fixed on magnet frame base are fixed on magnetic force post installing plate, institute
It is all the middle cylinder-shaped magnet with cavity to state the magnetic force post in magnetic force post matrix；The rack for test tube is main by test tube frame body
And it is vertically installed in the cuvette cavity matrix for holding test tubes of test tube frame body upper end, the cuvette cavity and the institute with cavity
State magnetic force post and match correspondence one by one, the lower end of the cuvette cavity can be inserted into the top of the cavity of the magnetic force post.
Further say, the inner ring of the magnetic force post is that N poles, outer ring are S poles or inner ring is that S poles, outer ring are N poles.
Further say, the cylinder-shaped magnet is alnico magnets.
Further say, the rack for test tube includes superposed test tube and places plate and the side plate positioned at surrounding, the examination
Pipe places the top that plate is located at the side plate, and the test tube places plate and the side plate integrated injection molding, the side plate bottom
Sleeve and it can be fixed on the magnet frame base.
Further say, it is 8,*12 96 hole shirt rim plates or 4*6 24 hole shirt rim plates that the test tube, which places plate,.
Further say, the cuvette cavity of the rack for test tube can place 200 μ l test tube.
Further say, the rack for test tube is the rack for test tube of acrylic injection moulding integrated molding.
The beneficial effects of the utility model are：The utility model provides a kind of nucleic acid purification magnetic frame, the magnetic frame
For carrying out magnetic to magnetisable material in test tube, the magnetic frame includes magnet frame and the examination being removably mounted to above magnet frame
Pipe support, magnet frame is provided with the magnetic force post matrix that uniform vertical is fixed on magnetic force post installing plate, and magnetic force post is with cavity
Cylinder-shaped magnet, the cuvette cavity bottom of holding test tubes just inserted in the cavity of cylinder-shaped magnet, and test tube lower end is by magnetic force post
Surround, therefore can comprehensive offer magnetic force so that the magnetic force of magnetic is greatly enhanced, and the absorption affinity to magnetic bead does not decline with enrichment with magnetic bead
Subtract so that magnetic bead has stronger adhesive force, so as to strengthen the adaptability of magnetic bead collection, greatly improve nucleic acid extraction efficiency, keep away
Exempt from magnetic force post magnetic too small and cause magnetic bead reunion, magnetic time length, the halfway phenomenon of magnetic.
Meanwhile, comprehensive magnetic force accelerates magnetic bead magnetic suck speed, so as to improve automation equipment paramagnetic particle method nucleic acid extraction
Adaptivity and processing speed, save the operating time, improve paramagnetic particle method nucleic acid extraction, the efficiency of purifying.Further, this magnetic frame
With many magnetic force posts, it is adapted to the multiple sample of nucleic acid of extraction purification, greatly improves nucleic acid extraction efficiency.
Described above is only the general introduction of technical solutions of the utility model, in order to better understand skill of the present utility model
Art means, and being practiced according to the content of specification, with preferred embodiment of the present utility model and coordinate accompanying drawing detailed below
Describe in detail bright as after.
Brief description of the drawings
Fig. 1 is the overall cross-sectional view of magnetic frame of the present utility model；
Fig. 2 is the overall schematic of magnet frame of the present utility model；
Fig. 3 is the top view of magnet frame of the present utility model；
Each several part mark is as follows in accompanying drawing：
Magnet frame 1, rack for test tube 2, magnet frame base 11, magnetic force post installing plate 12, magnetic force post 13, cuvette cavity 21, test tube are put
Put plate 22 and side plate 23.
Embodiment
Illustrate embodiment of the present utility model below by way of particular specific embodiment, those skilled in the art can
Advantage of the present utility model and effect are understood by content disclosed in the present specification easily.The utility model can also it is other not
Same mode is practiced, i.e. under the scope of without departing substantially from disclosed in the utility model, can give different modifications and change.
Embodiment：A kind of nucleic acid purification magnetic frame, includes the He of magnet frame 1 there is provided one kind as shown in Figure 1, Figure 2 and Figure 3
The rack for test tube 2 of the top of magnet frame 1 is removably mounted to, the magnet frame 1 includes magnet frame base 11, is fixed on magnet frame base
The magnetic force post matrix that magnetic force post installing plate 12 and uniform vertical on 11 are fixed on magnetic force post installing plate 12, the magnetic force post square
Magnetic force post 13 in battle array is all the middle cylinder-shaped magnet with cavity；The rack for test tube 2 mainly includes test tube frame body and hung down
The straight cuvette cavity matrix for holding test tubes for being arranged at test tube frame body upper end, the cuvette cavity 21 is with having the described of cavity
Magnetic force post 13 matches correspondence one by one, and the lower end of the cuvette cavity 21 can be inserted into the top of the cavity of the magnetic force post 13.
The inner ring of the magnetic force post 13 is that N poles, outer ring are S poles or inner ring is that S poles, outer ring are N poles.
The cylinder-shaped magnet is alnico magnets.
The rack for test tube 2 includes superposed test tube and places plate 22 and the side plate 23 positioned at surrounding, and the test tube is placed
Plate 22 is located at the top of the side plate 23, and the test tube places plate 22 and the integrated injection molding of side plate 23, the side plate 23
Bottom sleeve and can be fixed on the magnet frame base 11.
The test tube places the 96 hole shirt rim plates that plate 22 is 8*12.
The cuvette cavity 21 of the rack for test tube 2 can place 200 μ l test tube.
The rack for test tube 2 is the rack for test tube of acrylic injection moulding integrated molding.
The present embodiment has 96 hole shirt rim Ban Ji96 holes magnetic frames, you can placing 200 μ l cuvette cavity can at most put simultaneously
96 samples are put, but are used as the modification and replacement for being originally embodiment, however not excluded that other hole counts and other model test tubes can be placed
Magnetic frame, such as constitute 24 hole magnetic frames using 4*6 24 hole shirt rim plates.
Operation principle of the present utility model is as follows：
First with cell pyrolysis liquid so that the abundant separate out of nucleic acid, adds after magnetic bead and cause nucleic acid fully to be tied with magnetic bead
Close, test tube is placed in cuvette cavity, magnetic bead is adsorbed in the comprehensive magnetic field formed by the inner ring of magnetic force post so that the magnetic force of magnetic
Greatly enhance, the absorption affinity to magnetic bead does not decay with enrichment with magnetic bead so that magnetic bead has stronger adhesive force, so as to strengthen magnetic
The adaptability that pearl is collected so that magnetic bead is uniformly sufficiently adsorbed on wall in vitro, greatly improves nucleic acid extraction efficiency, it is to avoid magnetic
Power post magnetic is too small and causes magnetic bead reunion, magnetic time length, the magnetic phenomenon that thorough or magnetic bead is not lost.
Embodiment of the present utility model is the foregoing is only, the scope of the claims of the present utility model is not thereby limited, it is every
The equivalent structure made using the utility model specification and accompanying drawing content, or directly or indirectly it is used in other related technologies
Field, is similarly included in scope of patent protection of the present utility model.
Claims (7)
1. a kind of nucleic acid purification magnetic frame, it is characterised in that：Including magnet frame and the examination being removably mounted to above magnet frame
Pipe support, the magnet frame includes magnet frame base, the magnetic force post installing plate being fixed on magnet frame base and uniform vertical and fixed
Magnetic force post in the magnetic force post matrix on magnetic force post installing plate, the magnetic force post matrix is all the middle cylinder with cavity
Magnet；The rack for test tube is main by test tube frame body and to be vertically installed in the test tube for holding test tubes of test tube frame body upper end
Chamber matrix, the cuvette cavity matches corresponding, the pluggable institute in lower end of the cuvette cavity with the magnetic force post with cavity one by one
State the top of the cavity of magnetic force post.
2. nucleic acid purification magnetic frame according to claim 1, it is characterised in that：The inner ring of the magnetic force post is N poles, outside
Circle is S poles or inner ring is that S poles, outer ring are N poles.
3. nucleic acid purification magnetic frame according to claim 1, it is characterised in that：The cylinder-shaped magnet is permanent magnetic
Iron.
4. nucleic acid purification magnetic frame according to claim 1, it is characterised in that：The rack for test tube includes superposed
Test tube places plate and the side plate positioned at surrounding, and the test tube places the top that plate is located at the side plate, the test tube place plate with
The side plate integrated injection molding, the side plate bottom sleeve and can be fixed on the magnet frame base.
5. nucleic acid purification magnetic frame according to claim 1, it is characterised in that：The test tube places 96 that plate is 8*12
24 hole shirt rim plates of hole shirt rim plate or 4*6.
6. nucleic acid purification magnetic frame according to claim 1, it is characterised in that：The cuvette cavity of the rack for test tube can be placed
200 μ l test tube.
7. nucleic acid purification magnetic frame according to claim 1, it is characterised in that：The rack for test tube is acrylic injection one
Body formed rack for test tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621406454.XU CN206375924U (en) | 2016-12-21 | 2016-12-21 | Nucleic acid purification magnetic frame |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621406454.XU CN206375924U (en) | 2016-12-21 | 2016-12-21 | Nucleic acid purification magnetic frame |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206375924U true CN206375924U (en) | 2017-08-04 |
Family
ID=59407222
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621406454.XU Active CN206375924U (en) | 2016-12-21 | 2016-12-21 | Nucleic acid purification magnetic frame |
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|---|---|
| CN (1) | CN206375924U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109336950A (en) * | 2018-11-23 | 2019-02-15 | 嘉兴领宇科技有限公司 | A kind of magnetic frame |
-
2016
- 2016-12-21 CN CN201621406454.XU patent/CN206375924U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109336950A (en) * | 2018-11-23 | 2019-02-15 | 嘉兴领宇科技有限公司 | A kind of magnetic frame |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190614 Address after: 102600 Beijing Daxing District Beijing Economic and Technological Development Zone No. 156 Courtyard Building 401 Jinghai Fourth Road Patentee after: Beijing Qingke Biotechnology Co.,Ltd. Address before: 102209 No. 64 Litang Road, Beiqijiazhen, Changping District, Beijing Patentee before: BEIJING TSINGKE XINYE BIOLOGICAL TECHNOLOGY CO.,LTD. |
|
| TR01 | Transfer of patent right | ||
| CP03 | Change of name, title or address |
Address after: 100176 Floor 6, West Half Unit, Building 3, Yard 105, Jinghai 3rd Road, Daxing District, Beijing Economic-Technological Development Area Patentee after: Beijing Qingke Biotechnology Co.,Ltd. Address before: 401, building 5, No. 156, Jinghai 4th Road, Beijing Economic and Technological Development Zone, Daxing District, Beijing 102600 Patentee before: Beijing Qingke Biotechnology Co.,Ltd. |
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| CP03 | Change of name, title or address |