CN105316212A - Magnetic bead method nucleic acid extraction treatment equipment - Google Patents
Magnetic bead method nucleic acid extraction treatment equipment Download PDFInfo
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- CN105316212A CN105316212A CN201410360456.9A CN201410360456A CN105316212A CN 105316212 A CN105316212 A CN 105316212A CN 201410360456 A CN201410360456 A CN 201410360456A CN 105316212 A CN105316212 A CN 105316212A
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Abstract
The present invention relates to magnetic bead method nucleic acid extraction treatment equipment, which comprises a magnetic bead box, sample tracks, a thermal drying gun, a waste bead box, a washing tank, a vacuum suction table, a magnetic suction table and a machine table, and is characterized in that the magnetic bead box, the sample tracks, the thermal drying gun, the waste bead box, the washing tank, the vacuum suction table and the magnetic suction table are arranged on the machine table, the vacuum suction table and the magnetic suction table are arranged in a side-by-side manner, the thermal drying gun is arranged between the vacuum suction table and the magnetic suction table, the magnetic bead box is arranged on the outer side of the vacuum suction table, the waste bead box is arranged on the outer side of the magnetic suction table, the washing tank is arranged on the outer side of the waste bead box, the sample tracks are arranged in front of the vacuum suction table and the magnetic suction table, the washing tank selects an ultrasonic washing tank, and a stepping motor drives the vacuum suction table and the magnetic suction table to rotate. According to the present invention, the magnetic bead in the magnetic bead box is adsorbed through vacuumizing so as to provide characteristics of convenience and high efficiency, the magnetic bead in the sample is subjected to magnetic adsorption so as to prevent the reaction liquid in the sample from suction, and the adsorption and the release of the batch of the magnetic beads completely achieve automation; and the equipment has characteristics of simple structure and easy use.
Description
Technical field
The present invention relates to biological technical field, specifically paramagnetic particle method nucleic acid extraction treatment facility, be mainly used on paramagnetic particle method nucleic acid extraction instrument.
Background technology
paramagnetic particle method nucleic acid extraction kit, be the new high-tech product that both bio-science and nanometer material science are unified, be the once large breakthrough of China's nucleic acid extraction purification technique, it solves the situation of the long-term dependence on import of China's nucleic acid extraction purifying thoroughly.
paramagnetic particle method nucleic acid extraction, there is the advantage that traditional DNA extraction method is incomparable, be mainly reflected in: 1. can realize automatization, operate in enormous quantities, the nucleic acid automatic extracting instrument in existing 96 holes at present, the process to 96 samples can be realized with the extraction time of a sample, meet the high-throughout operational requirement of biology, can carry out when communicable disease is broken out tackling timely fast, this feature makes traditional method too far behind to catch up; 2. simple to operate, the used time is short, whole extraction flow process only has four steps, mostly can complete in 36-40 minute; 3. safety non-toxic, does not use the toxic reagents such as the benzene in traditional method, chloroform, reduces to minimum, meet modern environmental protection concept completely to the injury of experiment operator; 4. the specific binding of magnetic bead and nucleic acid makes that the nucleic acid purity that extracts is high, concentration is large.
the research of present magnetic bead extraction equipment is mainly carried out on the complete machine of magnetic bead extraction, its research direction be mainly the steps such as cracking, washing, wash-out reagent composition and environmental Kuznets Curves on, its magnetic bead belongs to expendable consumed product mostly, also do not have at present one can for batch sample, realize homodyne mistake and automatically put magnetic bead, and upon reaction completion, realize the automatic equipment of the wrong automatic clamping and placing magnetic bead of homodyne.
Summary of the invention
the present invention is above technical problem just, provides one for batch sample, can to realize homodyne mistake and automatically put magnetic bead, and upon reaction completion, realizes the automatization paramagnetic particle method extraction process equipment of the wrong automatic clamping and placing magnetic bead of homodyne.
the present invention is achieved through the following technical solutions:
paramagnetic particle method nucleic acid extraction treatment facility, comprise magnetic bead box, sample track, heat dries rifle, give up pearl box, rinse bath, vacuum suction table, magnetic platform, board, it is characterized in that magnetic bead box, sample track, heat baking rifle, useless pearl box, rinse bath, vacuum suction table, magnetic platform are arranged on board, vacuum suction table, magnetic platform are arranged side by side, heat is set between vacuum suction table and magnetic platform and dries rifle, magnetic bead box is arranged on outside vacuum suction table, magnetic platform arranged outside is given up pearl box, useless pearl box arranged outside rinse bath, vacuum suction table, magnetic platform front are provided with sample track.Rinse bath selects ultrasonic cleaner.Vacuum suction table, magnetic platform are by step motor driven rotary.
when the present invention uses, abundant magnetic bead can be placed in magnetic bead box, when the suction inlet of vacuum suction table passes through above step motor driven rotary to magnetic bead box, suction unit moves down, vacuum suction table vacuumizes, after making the suction nozzle on vacuum suction table all suct magnetic bead, suction unit is moved, then the suction unit part of vacuum suction table rotates again above sample track, suction unit moves down, when puncturing the sample sealing in sample track when suction unit, vacuum suction table stops vacuumizing, magnetic bead falls under gravity, then suction unit again on move, complete and put magnetic bead step, after reacting completely, sample is put into again the sample track of magnetic platform side, the suction unit part of magnetic platform is displaced downwardly to desired part, pass through energizing magnetic, and magnetic is passed on the suction nozzle of magnetic platform, magnetic bead in sample holds by suction nozzle, then suction unit is moved, magnetic platform moves to useless pearl box under the drive of step motor, the power-off of magnetic platform, suction nozzle on magnetic platform loses magnetism, magnetic bead just falls under gravity, then suction head portion is advanced in rinse bath by cylinder and cleans by magnetic platform, again suction head portion is moved to heat baking rifle position to dry, then reset and wait for action next time.The present invention by the magnetic bead in vacuumizing and adsorbing magnetic bead box, convenience and high-efficiency, then by the magnetic bead in magnetic absorption sample, prevent the reaction solution siphoned away in sample, and make the magnetic bead of batch inhale to discharge and entirely realize automatization.
structure of the present invention is simple, easy to use.
Accompanying drawing explanation
in accompanying drawing, Fig. 1 is structural representation of the present invention, wherein:
1-magnetic bead box, 2-sample track, 3-heat dries rifle, 4-useless pearl box, 5-rinse bath, 6-vacuum suction table, 7-magnetic platform, 8-board.
Embodiment
below in conjunction with specific embodiment, the present invention will be further described.
paramagnetic particle method nucleic acid extraction treatment facility, comprise magnetic bead box 1, sample track 2, heat dries rifle 3, useless pearl box 4, rinse bath 5, vacuum suction table 6, magnetic platform 7, board 8, it is characterized in that magnetic bead box 1, sample track 2, heat dries rifle 3, useless pearl box 4, rinse bath 5, vacuum suction table 6, magnetic platform 7 is arranged on board 8, vacuum suction table 6, magnetic platform 7 is arranged side by side, heat is set between vacuum suction table 6 and magnetic platform 7 and dries rifle 3, magnetic bead box 1 is arranged on outside vacuum suction table 6, magnetic platform 7 arranged outside is given up pearl box 4, useless pearl box 4 arranged outside rinse bath 5, vacuum suction table 6, magnetic platform 7 front is provided with sample track 2.Rinse bath 5 selects ultrasonic cleaner 5.Vacuum suction table 6, magnetic platform 7 are by step motor driven rotary.
when the present invention uses, abundant magnetic bead can be placed in magnetic bead box 1, when the suction inlet of vacuum suction table 6 passes through above step motor driven rotary to magnetic bead box 1, suction unit moves down, vacuum suction table 6 vacuumizes, after making the suction nozzle on vacuum suction table 6 all suct magnetic bead, suction unit is moved, then the suction unit part of vacuum suction table 6 rotates again above sample track 2, suction unit moves down, when puncturing the sample sealing in sample track 2 when suction unit, vacuum suction table 6 stops vacuumizing, magnetic bead falls under gravity, then suction unit again on move, complete and put magnetic bead step, after reacting completely, sample is put into again the sample track 2 of magnetic platform 7 side, the suction unit part of magnetic platform 7 is displaced downwardly to desired part, pass through energizing magnetic, and magnetic is passed on the suction nozzle of magnetic platform 7, magnetic bead in sample holds by suction nozzle, then suction unit is moved, magnetic platform 7 moves to useless pearl box 4 under the drive of step motor, magnetic platform 7 power-off, suction nozzle on magnetic platform 7 loses magnetism, magnetic bead just falls under gravity, then suction head portion is advanced in rinse bath 5 by cylinder and cleans by magnetic platform 7, again suction head portion is moved to heat baking rifle 3 position to dry, then reset and wait for action next time.The present invention by the magnetic bead in vacuumizing and adsorbing magnetic bead box 1, convenience and high-efficiency, then by the magnetic bead in magnetic absorption sample, prevent the reaction solution siphoned away in sample, and make the magnetic bead of batch inhale to discharge and entirely realize automatization.
structure of the present invention is simple, easy to use.
Claims (4)
1. paramagnetic particle method nucleic acid extraction treatment facility, comprise magnetic bead box, sample track, heat dries rifle, give up pearl box, rinse bath, vacuum suction table, magnetic platform, board, it is characterized in that magnetic bead box, sample track, heat baking rifle, useless pearl box, rinse bath, vacuum suction table, magnetic platform are arranged on board, vacuum suction table, magnetic platform are arranged side by side, heat is set between vacuum suction table and magnetic platform and dries rifle, magnetic bead box is arranged on outside vacuum suction table, magnetic platform arranged outside is given up pearl box, useless pearl box arranged outside rinse bath, vacuum suction table, magnetic platform front are provided with sample track.
2. paramagnetic particle method nucleic acid extraction treatment facility according to claim 1, is characterized in that described rinse bath selects ultrasonic cleaner.
3. paramagnetic particle method nucleic acid extraction treatment facility according to claim 1, is characterized in that described vacuum suction table is by step motor driven rotary.
4. paramagnetic particle method nucleic acid extraction treatment facility according to claim 1, is characterized in that described magnetic platform is by step motor driven rotary.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410360456.9A CN105316212A (en) | 2014-07-28 | 2014-07-28 | Magnetic bead method nucleic acid extraction treatment equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410360456.9A CN105316212A (en) | 2014-07-28 | 2014-07-28 | Magnetic bead method nucleic acid extraction treatment equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105316212A true CN105316212A (en) | 2016-02-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410360456.9A Pending CN105316212A (en) | 2014-07-28 | 2014-07-28 | Magnetic bead method nucleic acid extraction treatment equipment |
Country Status (1)
| Country | Link |
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| CN (1) | CN105316212A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1554453A (en) * | 2003-12-25 | 2004-12-15 | 东南大学 | Nano magnetic particle in vivo collector and collecting method |
| CN201201943Y (en) * | 2008-04-08 | 2009-03-04 | 上海理工大学 | Nucleic acid extracting system apparatus |
| CN103897987A (en) * | 2014-02-18 | 2014-07-02 | 中国农业大学 | Automatic extraction device and method of nucleic acid based on nano magnetic beads |
-
2014
- 2014-07-28 CN CN201410360456.9A patent/CN105316212A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1554453A (en) * | 2003-12-25 | 2004-12-15 | 东南大学 | Nano magnetic particle in vivo collector and collecting method |
| CN201201943Y (en) * | 2008-04-08 | 2009-03-04 | 上海理工大学 | Nucleic acid extracting system apparatus |
| CN103897987A (en) * | 2014-02-18 | 2014-07-02 | 中国农业大学 | Automatic extraction device and method of nucleic acid based on nano magnetic beads |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160210 |
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| WD01 | Invention patent application deemed withdrawn after publication |