CN214918846U - Simple plate washing device for enzyme-linked immunosorbent assay - Google Patents

Simple plate washing device for enzyme-linked immunosorbent assay Download PDF

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Publication number
CN214918846U
CN214918846U CN202120698198.0U CN202120698198U CN214918846U CN 214918846 U CN214918846 U CN 214918846U CN 202120698198 U CN202120698198 U CN 202120698198U CN 214918846 U CN214918846 U CN 214918846U
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shower
enzyme
linked immunosorbent
immunosorbent assay
plate
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CN202120698198.0U
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Chinese (zh)
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王�锋
颜文卿
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Suzhou Feikang Biological Pharmaceutical Co ltd
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Suzhou Feikang Biological Pharmaceutical Co ltd
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Abstract

The utility model discloses a simply wash board device for enzyme-linked immunosorbent assay, including washing the storehouse, set up the tool that is used for fixing a position the ELIAS plate in washing the storehouse, spray the mechanism, drive 180 tilting mechanism and elevating system of tool upset, spray the mechanism including establishing the shower in washing the storehouse, pass through the water pump of pipe connection with the one end of shower, pass through the washing liquid bottle of pipe connection with the water pump, the below of shower is connected with a plurality of shower heads, the quantity in the hole of shower head is unanimous with the quantity in the ELIAS plate, and the diameter in the hole on the ELIAS plate is less than to the diameter of shower, elevating system and shower connection. The utility model provides a simple and easy plate cleaning device for enzyme-linked immunosorbent assay washes the ELIAS plate through spraying the mechanism, thereby rethread tilting mechanism drives ELIAS plate upset 180 and inverts and clear away the water in the ELIAS plate, has realized the purpose of self-cleaning ELIAS plate, has not only improved work efficiency, and has practiced thrift manpower and time.

Description

Simple plate washing device for enzyme-linked immunosorbent assay
Technical Field
The utility model relates to the technical field of chargers, in particular to a simple plate washing device for enzyme-linked immunosorbent assay.
Background
Enzyme-linked immunosorbent assays (ELISAs) are common antibody or antigen detection methods. ELISA is based on the determination of the enzyme-catalyzed reaction of those immune complexes by the associated enzyme. The antibody may be immobilized on a surface-coated plastic plate, so that by simply washing the plate, the immune complex is readily separated from the other components, and the assay is performed.
In the enzyme-linked immunosorbent assay and detection process, an ELISA plate is often used as a reaction container and an enzyme carrier, a reaction reagent and an experimental sample are usually added into each micropore in the assay, the reaction is carried out in an environment simulating the temperature of a human body, reactants in the micropores of the ELISA plate which reacts are washed away after the reaction is finished, substances which react and are combined on the walls of the micropores are left, the process is plate washing, and other reaction substrates can be added after the plate washing to carry out the next experimental process.
The washing liquid is repeatedly injected into the micropores to be washed in the existing plate washing process, and the washing liquid is beaten after being washed, so that the washing liquid remaining in the micropores is minimum, the manual plate washing mode wastes manpower and time, the working efficiency is low, and in addition, cross contamination among the micropores is easy.
SUMMERY OF THE UTILITY MODEL
In order to overcome the defects of the prior art, the utility model discloses a simple plate washing device for enzyme-linked immunosorbent assay.
In order to achieve the above object, the utility model discloses a following technical scheme realizes:
the utility model discloses a simply wash board device for enzyme-linked immunosorbent assay, including wash the storehouse, set up in wash the interior tool that is used for fixing a position the ELIAS plate of storehouse, spray mechanism, drive tool upset 180 tilting mechanism and elevating system, the tool is located spray mechanism's below, spray mechanism is including establishing spray pipe in the washing storehouse, with the one end of spray pipe pass through pipe connection's water pump, with the water pump passes through pipe connection's washing liquid bottle, the below of spray pipe is connected with a plurality of shower heads, the quantity of shower head is unanimous with the quantity in the hole of ELIAS plate, and the diameter of spray pipe is less than the diameter in the hole on the ELIAS plate, elevating system with the shower is connected.
Preferably, the lifting mechanism comprises a lifting cylinder fixedly arranged above the cleaning bin, and a telescopic rod of the lifting cylinder is fixedly connected with the spray pipe.
Further preferably, the lifting cylinder is a cylinder with a guide rail.
Preferably, the side of the spray header is provided with a plurality of small holes, and the tail end of the spray header is also provided with small holes.
Preferably, the jig comprises a substrate and a slot arranged on the substrate, wherein one side of the slot is opened and is used as an insertion port of the elisa plate.
Preferably, the slots and the two side walls perpendicular to the insertion holes extend inwards to form press blocks respectively, and the press blocks are used for pressing and attaching the edge of the top end face of the elisa plate.
Preferably, the turnover mechanism comprises a motor and a rotating shaft, the rotating shaft penetrates through the bottom of the jig and is connected with the jig in a rotating mode, the motor is arranged outside the cleaning bin, and the motor is fixedly connected with the rotating shaft.
Preferably, be provided with the waste liquid mouth that is used for the waste liquid outflow on the lateral wall in washing storehouse, waste liquid mouth and waste liquid collection device intercommunication.
Compared with the prior art, the utility model discloses an at least, following advantage has:
the utility model provides a simple and easy plate cleaning device for enzyme-linked immunosorbent assay washes the ELIAS plate through spraying the mechanism, thereby rethread tilting mechanism drives ELIAS plate upset 180 and inverts and clear away the water in the ELIAS plate, has realized the purpose of self-cleaning ELIAS plate, has not only improved work efficiency, and has practiced thrift manpower and time.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly described below.
Fig. 1 is a schematic structural view of a simple plate washing device for enzyme-linked immunosorbent assay disclosed in the embodiment of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the following embodiments and the accompanying drawings so that those skilled in the art can implement the invention with reference to the description.
Referring to fig. 1, the embodiment of the utility model discloses a simple and easy plate cleaning device for enzyme-linked immunosorbent assay, including cleaning storehouse 1, set up in cleaning storehouse 1 and be used for positioning the tool 2 of ELIAS plate, spray mechanism 3, drive tool 2 upset 180 tilting mechanism 4 and elevating system 5, tool 2 is located the below that sprays mechanism 3, spray mechanism 3 includes setting up shower 31 in cleaning storehouse 1, the water pump 33 that passes through the pipeline 32 with one end of shower 31 and be connected, the washing liquid bottle 34 that passes through the pipeline 32 with water pump 33 and be connected, the below of shower 31 is connected with a plurality of shower heads 34, the quantity of shower head 34 is unanimous with the quantity of the hole of ELIAS plate K (ELIAS plate K is 96, in order to simplify the structure of ELIAS plate K in the figure, only show 8 holes, the corresponding number of shower head is also 8, actual ELIAS plate K has 96 holes, the number of the spray heads is correspondingly 96), the diameter of the spray pipe 31 is smaller than that of the holes on the ELISA plate K, and the lifting mechanism 5 is connected with the spray pipe 31.
The lifting mechanism 5 comprises a lifting cylinder 51 fixedly arranged above the cleaning bin 1, and a telescopic rod of the lifting cylinder 51 is fixedly connected with the spray pipe 31. Wherein, the cylinder of taking the guide rail is adopted to lift cylinder 51, and stability is better.
The side of shower head 34 is provided with a plurality of apertures, and the end of shower head also has and is provided with the aperture, all sets up the aperture and can increase the washing liquid jet volume in the both sides of shower head and end for wash more fully.
The jig 2 comprises a base plate 21 and a slot 22 arranged on the base plate 21, one side of the slot 22 is opened and is used as an insertion opening of the ELISA plate, and when the ELISA plate is pushed to the bottom from the insertion opening, the ELISA plate is just completely positioned in the slot 22. Certainly, the inserting port can also be provided with a sealing plate for sealing the ELISA plate, so that the stability of the ELISA plate in the jig 2 is further improved, and the ELISA plate is prevented from being separated.
The upper parts of two side walls of the slot 22 perpendicular to the insertion opening extend inwards to form a pressing block 221, the pressing block 221 is used for pressing and attaching the edge of the top end face of the ELISA plate, and the pressing block 221 plays a role in positioning the Z-axis direction freedom degree of the ELISA plate.
The turnover mechanism 4 comprises a motor 41 and a rotating shaft 42, the rotating shaft 42 penetrates through the bottom of the jig 2 and is connected with the jig 2 in a rotating mode, the motor 41 is arranged outside the cleaning bin 1, and the motor 41 is fixedly connected with the rotating shaft 42. The motor 41 drives the rotating shaft to rotate 180 degrees, the rotating shaft 42 drives the jig 2 to rotate 180 degrees, and then the elisa plate is driven to rotate 180 degrees.
Be provided with the waste liquid mouth 11 that is used for the waste liquid outflow on the lateral wall in washing storehouse 1, waste liquid mouth 11 and waste liquid collection device intercommunication.
When the simple plate washing device for enzyme-linked immunosorbent assay provided by the embodiment of the utility model works, the lifting cylinder 51 drives the spray pipe 31 to move to the upper part of the ELISA plate, so that the spray head is positioned in the small hole of the ELISA plate; the cleaning liquid in the cleaning liquid bottle is pumped into the spray pipe 31 by the water pump 33 and sprayed into the small holes of the ELISA plate by the spray head for cleaning; after the washing, the spray header stops spraying, the motor 41 drives the rotating shaft 42 to rotate, and then the elisa plate is transferred to rotate 180 degrees, the small holes on the elisa plate face downwards, so that water in the small holes can slide down, and the aim of removing water is fulfilled; the washing is repeated for several times and the water is removed for several times, thereby achieving the purpose of fully washing the ELISA plate.
Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (8)

1. A simple plate washing device for enzyme-linked immunosorbent assay is characterized in that: including the washing storehouse, set up in the washing storehouse be used for the tool of location ELIAS plate, spray mechanism, drive tool upset 180 tilting mechanism and elevating system, the tool is located spray mechanism's below, spray mechanism is including establishing shower in the washing storehouse, with the one end of shower passes through pipe connection's water pump, with the water pump passes through pipe connection's washing liquid bottle, the below of shower is connected with a plurality of shower heads, the quantity in shower head is unanimous with the quantity in the hole of ELIAS plate, and the diameter of shower is less than the diameter in the hole on the ELIAS plate, elevating system with the shower is connected.
2. The simple plate washing device for enzyme-linked immunosorbent assay according to claim 1, wherein: the lifting mechanism comprises a lifting cylinder fixedly arranged above the cleaning bin, and a telescopic rod of the lifting cylinder is fixedly connected with the spray pipe.
3. The simple plate washing device for enzyme-linked immunosorbent assay according to claim 2, wherein: the lifting cylinder is a cylinder with a guide rail.
4. The simple plate washing device for enzyme-linked immunosorbent assay according to claim 1, wherein: the side of the spray header is provided with a plurality of small holes, and the tail end of the spray header is also provided with small holes.
5. The simple plate washing device for enzyme-linked immunosorbent assay according to claim 1, wherein: the jig comprises a substrate and a slot arranged on the substrate, wherein one side of the slot is opened and is used as an insertion port of the ELISA plate.
6. The simple plate washing device for enzyme-linked immunosorbent assay according to claim 5, wherein: the upper parts of two side walls of the slot, which are vertical to the insertion opening, extend inwards to form a pressing block, and the pressing block is used for pressing and attaching the edge of the top end face of the ELISA plate.
7. The simple plate washing device for enzyme-linked immunosorbent assay according to claim 1, wherein: the turnover mechanism comprises a motor and a rotating shaft, the rotating shaft penetrates through the bottom of the jig and is connected with the jig in a rotating mode, the motor is arranged outside the cleaning bin, and the motor is fixedly connected with the rotating shaft.
8. The simple plate washing device for enzyme-linked immunosorbent assay according to claim 1, wherein: be provided with the waste liquid mouth that is used for the waste liquid outflow on the lateral wall in washing storehouse, waste liquid mouth and waste liquid collection device intercommunication.
CN202120698198.0U 2021-04-07 2021-04-07 Simple plate washing device for enzyme-linked immunosorbent assay Active CN214918846U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202120698198.0U CN214918846U (en) 2021-04-07 2021-04-07 Simple plate washing device for enzyme-linked immunosorbent assay

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202120698198.0U CN214918846U (en) 2021-04-07 2021-04-07 Simple plate washing device for enzyme-linked immunosorbent assay

Publications (1)

Publication Number Publication Date
CN214918846U true CN214918846U (en) 2021-11-30

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114160486A (en) * 2021-12-09 2022-03-11 河北燕孚科技有限公司 Plate washing machine for ELISA plate and washing method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114160486A (en) * 2021-12-09 2022-03-11 河北燕孚科技有限公司 Plate washing machine for ELISA plate and washing method thereof
CN114160486B (en) * 2021-12-09 2022-09-13 河北燕孚科技有限公司 Plate washing machine for ELISA plate and washing method thereof

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