CN114160486A - Plate washing machine for ELISA plate and washing method thereof - Google Patents
Plate washing machine for ELISA plate and washing method thereof Download PDFInfo
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- CN114160486A CN114160486A CN202111497713.XA CN202111497713A CN114160486A CN 114160486 A CN114160486 A CN 114160486A CN 202111497713 A CN202111497713 A CN 202111497713A CN 114160486 A CN114160486 A CN 114160486A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B13/00—Accessories or details of general applicability for machines or apparatus for cleaning
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B7/00—Cleaning by methods not provided for in a single other subclass or a single group in this subclass
- B08B7/02—Cleaning by methods not provided for in a single other subclass or a single group in this subclass by distortion, beating, or vibration of the surface to be cleaned
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F26—DRYING
- F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
- F26B25/00—Details of general application not covered by group F26B21/00 or F26B23/00
- F26B25/04—Agitating, stirring, or scraping devices
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F26—DRYING
- F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
- F26B5/00—Drying solid materials or objects by processes not involving the application of heat
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F26—DRYING
- F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
- F26B5/00—Drying solid materials or objects by processes not involving the application of heat
- F26B5/14—Drying solid materials or objects by processes not involving the application of heat by applying pressure, e.g. wringing; by brushing; by wiping
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- Mechanical Engineering (AREA)
- General Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Cleaning By Liquid Or Steam (AREA)
- Inking, Control Or Cleaning Of Printing Machines (AREA)
Abstract
The invention relates to a plate washing machine for an ELISA plate, which comprises: a fixed part; the cleaning part comprises an X-axis linear module, a first Z-axis linear module and a second Z-axis linear module, wherein the first Z-axis linear module is arranged on the X-axis linear module, an ELISA plate to be cleaned is arranged on the first Z-axis linear module, a cleaning needle bank communicated with cleaning liquid is arranged on the second Z-axis linear module, the cleaning needle bank is used for carrying out liquid injection cleaning on the ELISA plate, and a needle bank soaking groove for soaking the cleaning needle bank is arranged on a fixed seat; the dewatering part comprises a turnover oscillation part and a waste liquid tank, and further comprises a wiping part, a liquid injection pump and a liquid discharge pump, wherein the turnover oscillation part comprises a fixed box for mounting an enzyme label plate, a turnover part which is in transmission connection with the fixed box and can operate the turnover of the fixed box, and an oscillation part which is connected with the turnover part and can drive the turnover part to move up and down to oscillate and dewater. The invention can automatically clean the ELISA plate, and has the advantages of integrated cleaning and dehydration and good cleaning effect.
Description
Technical Field
The invention relates to the technical field of medical equipment, in particular to a plate washing machine for an ELISA plate and a method for washing the ELISA plate.
Background
The microplate washer is used for specially washing the microplate, and is widely applied to washing of the microplate in hospitals, blood stations, epidemic prevention stations, reagent factories, research institutions and the like at present.
Along with the development of science and technology, wash the trigger and also develop to automatic formula by the most primitive manual formula, among the prior art, automatic formula washes the trigger and can independently set for through the procedure and wash the number of times more, and wash the back and realize through inhaling water again, nevertheless inhale water the in-process and block up the pinhole easily, and wash the effect not good enough.
Disclosure of Invention
In order to solve the problems, the invention provides a plate washing machine for an ELISA plate, which can automatically wash the ELISA plate, can automatically dehydrate the ELISA plate, is integrally finished and has good washing effect.
The technical scheme adopted by the invention is as follows: a plate washer for an microplate comprising:
the fixing part comprises a fixing seat and an outer shell arranged on the fixing seat, a single chip microcomputer control board is arranged in the outer shell, and a touch screen which is arranged on the outer shell and is communicated with the single chip microcomputer control board through serial port communication;
the cleaning part comprises an X-axis linear module arranged on the fixed seat and a first Z-axis linear module arranged on the X-axis linear module, and an ELISA plate to be cleaned is arranged on the first Z-axis linear module; the enzyme label plate cleaning device is characterized by further comprising a second Z-axis linear module arranged on the fixed seat, a cleaning pin row communicated with cleaning liquid is arranged on the second Z-axis linear module, the cleaning pin row is used for carrying out liquid injection cleaning on the enzyme label plate, and a pin row soaking groove for soaking the cleaning pin row is arranged on the fixed seat;
the dewatering part comprises an overturning oscillating part and a waste liquid groove which is arranged on the fixed seat and used for receiving the cleaning waste liquid of the ELISA plate; the rotary enzyme plate cleaning device is characterized by further comprising a cleaning part installed on the fixing seat, a liquid injection pump and a liquid discharge pump installed on the fixing seat, wherein the turnover oscillating part comprises a fixing box for installing the enzyme plate, an overturning part which is in transmission connection with the fixing box and can operate the fixing box to overturn, and an oscillating part which is connected with the overturning part and can drive the overturning part to reciprocate up and down to oscillate and dewater, the cleaning part comprises a paper supporting frame and is arranged on wiping paper on the paper supporting frame, and the rotary enzyme plate cleaning device further comprises a paper replacing component for moving and replacing the wiping paper.
As a further limitation to the above technical solution, the oscillating portion includes a first mounting block connected to the first Z-axis linear module, and an oscillating motor mounted on the first mounting block; still include with the oscillating motor via the first transmission shaft of first shaft coupling transmission connection, and connect in the epaxial eccentric wheel of first transmission, still include one end connect in adjustable connecting rod on the eccentric wheel, the other end of adjustable connecting rod is connected with the second installation piece, install on the second installation piece upset portion, just the second installation piece spacing connect in on the first installation piece, with under the drive of adjustable connecting rod for reciprocating motion about the first installation piece.
As a further limitation to the above technical solution, the turning part includes a turning motor mounted on the second mounting block, and a second transmission shaft in transmission connection with the turning motor via a second coupling, and the fixed box is mounted on the second transmission shaft.
As a further limitation to the above technical solution, a slide rail is disposed on the first mounting block, and a sliding groove adapted to the slide rail is disposed on the second mounting block.
As a further limitation to the above technical solution, the paper changing assembly is adjacent to the paper support frame, and includes an installation seat installed on the fixing seat, a paper winding motor installed on the installation seat, a paper winding shaft connected to the paper winding motor in a transmission manner, and a paper discharging shaft connected to the installation seat in a rotation manner adjacent to the paper winding shaft, a plurality of auxiliary shafts for winding the wiping paper are installed on the paper support frame, and a paper distance measuring encoder is installed on one of the auxiliary shafts adjacent to the paper discharging shaft.
As a further limitation to the above technical solution, the number of the auxiliary shafts is four, and the auxiliary shafts are respectively a first auxiliary shaft, a second auxiliary shaft, a third auxiliary shaft and a fourth auxiliary shaft, wherein the first auxiliary shaft, the second auxiliary shaft and the third auxiliary shaft are mounted at one end of the paper support frame close to the paper discharge shaft and are arranged in a triangle, the fourth auxiliary shaft is mounted at the other end of the paper support frame, a winding path of the wiping paper is wound out from the paper discharge shaft side and sequentially passes through the first auxiliary shaft, the second auxiliary shaft, the fourth auxiliary shaft and the third auxiliary shaft, and then is wound in from the paper winding shaft.
As a further limitation to the above technical solution, the fixing box includes a box frame disposed in a U shape, and an end cover having one end hinged to an opening end of the box frame via a hinge, and a magnetic switch is installed between the other end of the end cover and the box frame.
The plate washer for the ELISA plate can realize automatic plate washing of the ELISA plate through the arrangement of the fixing part, the washing part and the dewatering part, and through the arrangement of the overturning oscillation part and the wiping part, under the action of the overturning oscillation part, the washing liquid on the ELISA plate is overturned to the waste liquid tank, is oscillated and dewatered through the oscillation part, and then is wiped to the residual liquid through the wiping part, so that the residual liquid amount is less than 2 mu l/hole, and the plate washer has high efficiency and high cleaning effect.
The invention also relates to a washing method of the ELISA plate, which uses the plate washing machine for the ELISA plate.
The technical scheme is that the method for cleaning the ELISA plate comprises the following steps:
A. clicking a 'start' button on the touch screen, and performing plate washing process control according to a parameter value preset by a program of the singlechip control plate;
B. when the plate washing is started, carrying out an initialization process of a plate washing machine for the ELISA plate;
C. reading various state parameters of the current program scheme, including parameters such as cleaning time, cleaning times, cleaning rows, holding time, oscillation and slapping times and the like;
D. carrying out an initial oscillation slapping process on the ELISA plate before liquid injection cleaning;
E. moving the cleaning needle bank to the needle bank soaking groove for needle cleaning, and then performing a first cleaning and dehydrating process of the ELISA plate;
F. and judging whether to continue the secondary cleaning and dehydrating process according to the cleaning times parameter until the cleaning and dehydrating process is completely finished.
As a further limitation to the above technical solution, in step D, the specific subdivision steps are as follows:
d1, controlling a turnover part, turning over the ELISA plate, starting an oscillation part, and beating the residue in the ELISA plate to a waste liquid tank;
d2, controlling the X-axis linear module and the first Z-axis linear module to move the ELISA plate to the wiping paper, starting the oscillating part, and continuously shooting residues in the ELISA plate to the wiping paper;
d3, controlling the X-axis linear module and the first Z-axis linear module to move the ELISA plate to the plate washing position, and finishing the initial oscillation slapping process. .
As a further limitation to the above technical solution, in step E, the specific subdivision steps are as follows:
e1, needle washing process: controlling a second Z-axis linear module to drive a cleaning needle bar to reach a needle bar soaking groove position, starting a liquid injection pump, injecting cleaning liquid into the needle bar soaking groove through a liquid injection needle of the cleaning needle bar, simultaneously starting a liquid discharge pump, and discharging the cleaning liquid in the needle bar soaking groove through a liquid discharge needle of the cleaning needle bar, wherein the cleaning time is t1 seconds;
e2, liquid injection cleaning process: after the needle washing process is finished, controlling the washing needle to be arranged at the first arranging hole of the enzyme label plate, starting a liquid injection pump to inject washing liquid, starting a liquid discharge pump to lead the washing liquid to the liquid discharge needle, and sucking liquid while injecting the liquid so as to ensure that the liquid level is uniform; after the first row of holes is filled with liquid, judging whether to continue filling the next row of holes according to the cleaning row number until the last row of holes is filled with cleaning liquid;
e3, standing process: filling the cleaning solution into the rows of holes of all the enzyme label plates, and standing the enzyme label plates for t2 seconds;
e4, dehydration: after standing time is up, starting the oscillating part to drive the ELISA plate to perform oscillating and slapping actions, slapping cleaning liquid on the ELISA plate into a waste liquid tank, moving the ELISA plate to a wiping paper after slapping for multiple times, starting the oscillating part, performing oscillating and slapping actions again, slapping the ELISA plate onto the wiping paper, and enabling the wiping paper to suck residual liquid away;
e5, after the above processes are completed, the action parts are reset, and the first washing and dewatering process of the ELISA plate is finished.
According to the washing method of the ELISA plate, the washing effect can be improved by performing the operation of the initial oscillation slapping flow on the ELISA plate before liquid injection washing, the blockage of the washing needle row can be better avoided by the operation of the needle washing process, the non-uniformity of liquid injection and liquid drainage is avoided, the residual liquid amount after washing of the ELISA plate is less than 2 mu l/hole by the cooperation of liquid injection washing, standing and dehydration, and the washing method has high efficiency and high cleaning effect.
Drawings
FIG. 1 is a schematic view of an assembly structure of a plate washer for an ELISA plate according to the present invention;
FIG. 2 is a schematic view of another angle-mounting structure of a plate washer for an ELISA plate according to the present invention;
FIG. 3 is a schematic view of a third angle assembly structure of a plate washer for an ELISA plate according to the present invention;
FIG. 4 is a schematic view of an assembly structure of the X-axis linear module and the first Z-axis linear module according to the present invention;
FIG. 5 is a schematic structural diagram of the inverted oscillating portion of the present invention;
FIG. 6 is a schematic view of another angular structure of the inverted oscillating portion of the present invention;
fig. 7 is a schematic view of the structure of the wiping section of the invention.
In the figure:
11-a fixed seat, 21-an X-axis linear module, 22-a first Z-axis linear module, 23-a supporting guide rail, 24-a supporting block, 25-a second Z-axis linear module, 26-a cleaning needle row, 31-a waste liquid groove, 32-a turnover oscillating part, 321-a fixed box, 3211-a box frame, 3212-an end cover, 3213-a magnetic suction switch, 322-a first mounting block, 3221-a sliding rail, 323-an oscillating motor, 324-a first coupling, 325-a first transmission shaft, 326-an eccentric wheel, 327-an adjustable connecting rod, 328-a second mounting block, 329-a turnover motor, 3210-a second coupling, 3214-a second transmission shaft, 33-a wiping part, 331-a paper supporting frame, 332-a mounting seat and 333-a paper rolling motor, 334-paper winding shaft, 335-paper placing shaft, 336-first auxiliary shaft, 337-second auxiliary shaft, 338-third auxiliary shaft, 339-fourth auxiliary shaft, 3310-paper distance measuring encoder, 34-liquid injection pump, 35-liquid discharge pump, 4-enzyme label plate and 5-needle row soaking tank.
Detailed Description
The invention is described in further detail below with reference to the figures and the embodiments.
Example one
By shown in fig. 1-3, a ELIAS plate is with washing trigger, its overall structure includes fixed part, washing portion and dehydration portion, and is concrete, and the fixed part includes fixing base 11 and installs the shell body on fixing base 11, and in this embodiment, fixing base 11 is the L type and assembles and form, in this embodiment, installs the singlechip control panel in the shell body to and install on the shell body, with the singlechip control panel between the touch-sensitive screen of UNICOM mutually via serial ports communication, and is concrete, the model of singlechip control panel is STC15F2K16S 2.
As shown in fig. 1 to 7, the cleaning portion includes an X-axis linear module 21 mounted on the fixing base 11 and a first Z-axis linear module 22 mounted on the X-axis linear module 21, and the elisa plate 4 to be cleaned is mounted on the first Z-axis linear module 22, in this embodiment, a support rail 23 is mounted on the fixing base 11, a support block 24 is slidably mounted on the support rail 23, the bottom of the first Z-axis linear module 22 is connected to the support block 24, and the side of the first Z-axis linear module 22 is mounted on the X-axis linear module 21; the cleaning part further comprises a second Z-axis linear module 25 mounted on the fixed base 11, a cleaning faller gill 26 communicated with a cleaning liquid is mounted on the second Z-axis linear module 25, the cleaning faller gill 26 is used for injecting liquid to the elisa plate 4 for cleaning, specifically, as shown in figure 2, in order to avoid the blockage of the cleaning faller gill 26, a faller gill soaking groove 5 for soaking the cleaning faller gill 26 is mounted on the fixed base 11, in the embodiment, the faller soaking groove 5 comprises a mounting frame mounted on the fixed base 11 and a groove body mounted on the mounting frame, a liquid injection needle and a liquid discharge needle are mounted on the cleaning faller gill 26, a liquid injection hole communicated with the liquid injection needle is mounted on the cleaning faller gill 26, a liquid injection pump 34 and a liquid suction pump are mounted on the fixed base 11, the liquid injection pump 34 is communicated with the liquid injection hole of the cleaning faller gill 26, and the liquid suction pump is respectively communicated with the groove body, a bracket is arranged on the fixed seat 11, and the second Z-axis linear module 25 is arranged on the bracket.
As shown in fig. 1 to 7, the dewatering section includes an inversion oscillating section 32, and a waste liquid tank 31 mounted on the holder 11 for receiving the washing waste liquid of the microplate 4; the rotary washing machine further comprises a wiping part 33 installed on the fixed seat 11, a liquid injection pump 34 and a liquid discharge pump 35 installed on the fixed seat 11, the turnover oscillating part 32 comprises a fixed box 321 for installing the elisa plate 4, a turnover part which is in transmission connection with the fixed box 321 and can operate the turnover of the fixed box 321, an oscillating part which is connected with the turnover part and can drive the turnover part to reciprocate up and down to oscillate and dewater, the wiping part 33 comprises a paper support 331 and wiping paper placed on the paper support 331, and a paper changing component for moving and changing the wiping paper is further included. In this embodiment, in order to facilitate taking and mounting of the elisa plate 4, the fixing box 321 includes a box frame 3211 disposed in a U shape, and an end cover 3212 having one end hinged to an opening end of the box frame 3211 via a hinge, and a magnetic attraction switch 3213 is installed between the other end of the end cover 3212 and the box frame 3211.
Specifically, the oscillating portion includes a first mounting block 322 connected to the first Z-axis linear module 22, and an oscillating motor 323 mounted on the first mounting block 322; the device further comprises a first transmission shaft 325 in transmission connection with the oscillating motor 323 through a first coupling 324, an eccentric wheel 326 connected to the first transmission shaft 325, an adjustable connecting rod 327 with one end connected to the eccentric wheel 326, the other end of the adjustable connecting rod 327 is connected with a second mounting block 328, a turnover part is mounted on the second mounting block 328, and the second mounting block 328 is in limit connection with the first mounting block 322 so as to be driven by the adjustable connecting rod 327 to move up and down in a reciprocating manner relative to the first mounting block 322. In this embodiment, for the purpose of adjusting the length, the adjustable connecting rod 327 includes a first rod connected to the eccentric 326, and a second rod having one end screwed to the first rod and the other end connected to the second mounting block 328.
Specifically, the turning part includes a turning motor 329 mounted on the second mounting block 328, and a second transmission shaft 3214 in transmission connection with the turning motor 329 via a second coupling 3210, the fixing box 321 is mounted on the second transmission shaft 3214, the first mounting block 322 is provided with a sliding rail 3221, and the second mounting block 328 is provided with a sliding groove adapted to the sliding rail 3221.
When the rotation and oscillation are needed, the turnover motor 329 is started, the turnover motor 329 drives the fixed box 321 and the elisa plate 4 fixed on the fixed box 321 to turn over to face the waste liquid tank 31 through the second transmission shaft 3214, then the oscillation motor 323 is started, the oscillation motor 323 drives the eccentric wheel 326 to rotate through the first transmission shaft 325, the adjustable connecting rod 327 is sequentially driven to move up and down, due to the arrangement of the sliding groove of the sliding rail 3221, the second mounting block 328 is further driven to move up and down relative to the first mounting block 322, and the eccentric wheel 326 rotates to drive the vibration of the second mounting block 328 to realize the dehydration.
In this embodiment, the paper changing assembly is disposed adjacent to the paper supporting frame 331, and includes a mounting seat 332 mounted on the fixing seat 11, a roll paper motor 333 mounted on the mounting seat 332, a roll paper shaft 334 drivingly connected to the roll paper motor 333, and a paper discharging shaft 335 rotatably connected to the mounting seat 332 adjacent to the roll paper shaft 334, a plurality of auxiliary shafts for winding the wiping paper are mounted on the paper supporting frame 331, and a paper distance measuring encoder 3310 is mounted on one of the auxiliary shafts adjacent to the paper discharging shaft 335.
Specifically, the number of the auxiliary shafts is four, and the auxiliary shafts are a first auxiliary shaft 336, a second auxiliary shaft 337, a third auxiliary shaft 338 and a fourth auxiliary shaft 339, wherein the first auxiliary shaft 336, the second auxiliary shaft 337 and the third auxiliary shaft 338 are mounted at one end of the paper support frame 331 close to the paper feeding shaft 335 and are arranged in a triangle, the fourth auxiliary shaft 339 is mounted at the other end of the paper support frame 331, the winding path of the wiping paper is wound from the paper feeding shaft 335 side and sequentially passes through the first auxiliary shaft 336, the second auxiliary shaft 337, the fourth auxiliary shaft 339 and the third auxiliary shaft 338 and then is wound around the paper winding shaft 334, and the paper distance measuring encoder 3310 is provided to ensure the replacement length of the wiping paper, specifically, the paper distance measuring encoder 3310 is mounted on the second auxiliary shaft 337, specifically, in order to prevent the rolls on the paper feeding shaft 335 and the paper winding shaft 334 from falling off, and hand adjusting thrust blocks are mounted at the ends of the paper feeding shaft 335 and the paper winding shaft 334.
Example two
A microplate cleaning method using the microplate cleaning machine of the first embodiment includes the steps of:
A. clicking a 'start' button on the touch screen, and performing plate washing process control according to a parameter value preset by a program of the singlechip control plate;
B. when the plate washing is started, carrying out an initialization process of a plate washing machine for the ELISA plate;
C. reading various state parameters of the current program scheme, including parameters such as cleaning time, cleaning times, cleaning rows, holding time, oscillation and slapping times and the like;
D. carrying out an initial oscillation slapping process on the ELISA plate 4 before liquid injection cleaning;
E. moving the cleaning needle bank 26 to the needle bank soaking groove 5 for needle cleaning, and then performing a first cleaning and dehydrating process of the ELISA plate 4;
F. and judging whether to continue the secondary cleaning and dehydrating process according to the cleaning times parameter until the cleaning and dehydrating process is completely finished.
In the embodiment, the touch screen is an eight-inch color liquid crystal touch screen and is used for displaying the state of the microplate 4 for the plate washing machine or inputting manual parameters, the touch screen is in data communication with the single chip microcomputer control panel through RS232, and the single chip microcomputer control panel has the functions of data storage, I/O control, serial port communication and the like.
In step D, the specific subdivision steps are as follows:
d1, controlling a turnover part, turning over the ELISA plate 4, starting an oscillation part, and beating the residue in the ELISA plate 4 to the waste liquid tank 31;
d2, controlling the X-axis linear module 21 and the first Z-axis linear module 22 to move the ELISA plate 4 to the wiping paper, starting the oscillating part, and continuously shooting residues in the ELISA plate 4 to the wiping paper;
d3, controlling the X-axis linear module 21 and the first Z-axis linear module 22 to move the ELISA plate 4 to the plate washing position, and ending the initial oscillation and patting process.
In step E, the specific subdivision steps are as follows:
e1, needle washing process: controlling the second Z-axis linear module 25 to drive the cleaning needle bar 26 to reach the position of the needle bar soaking groove 5, starting the liquid injection pump 34, injecting the cleaning liquid into the needle bar soaking groove 5 through the liquid injection needle of the cleaning needle bar 26, simultaneously starting the liquid discharge pump 35, and discharging the cleaning liquid in the needle bar soaking groove 5 through the liquid discharge needle of the cleaning needle bar 26, wherein the cleaning time is t1 seconds;
e2, liquid injection cleaning process: after the needle washing process is finished, controlling the washing needle row 26 to reach the first row hole of the ELISA plate 4, starting the liquid injection pump 34, injecting washing liquid, starting the liquid discharge pump 35 at the same time, leading to the liquid discharge needle, injecting liquid while sucking liquid, and ensuring that the liquid levels are uniform; after the first row of holes is filled with liquid, judging whether to continue filling the next row of holes according to the cleaning row number until the last row of holes is filled with cleaning liquid;
e3, standing process: filling the cleaning solution into the row holes of the ELISA plate 4, and standing the ELISA plate 4 for t2 seconds;
e4, dehydration: after standing time is up, starting the oscillating part to drive the ELISA plate 4 to carry out oscillating and slapping actions, slapping the cleaning solution on the ELISA plate 4 into the waste liquid tank 31, moving the ELISA plate 4 to the wiping paper after slapping for multiple times, starting the oscillating part, then carrying out oscillating and slapping actions, slapping the ELISA plate 4 onto the wiping paper, and enabling the wiping paper to suck residual liquid;
e5, after the above processes are completed, the action parts are reset, and the first washing and dewatering process of the ELISA plate 4 is finished.
Specifically, a peripheral driving/executing circuit is electrically connected with the singlechip control board, a liquid injection three-way valve and a liquid discharge valve are connected at the corresponding positions of the peripheral driving/executing circuit, and in the needle washing process, a liquid injection pump 34 is started, the liquid injection three-way valve is switched to be communicated with cleaning liquid, so that the cleaning liquid is injected into a groove body through a liquid injection needle, and the liquid injection needle is cleaned; meanwhile, the drain pump 35 is started, the drain valve is switched to be communicated with the drain needle, and the cleaning liquid injected into the tank body is pumped out through the drain needle and is discharged to the waste liquid barrel to clean the drain needle. The process is a needle washing process, the washing time, namely the duration time t1 is five seconds, and after the time is up, the corresponding liquid injection pump 34, liquid injection three-way valve, liquid discharge pump 35 and liquid discharge valve are closed.
In the liquid injection cleaning process, the cleaning needle bar 26 is controlled to be located at the first drain hole of the ELISA plate 4, the liquid injection pump 34 is started, the liquid injection three-way valve is switched to be communicated with cleaning liquid, cleaning liquid is injected, the liquid discharge pump 35 is started simultaneously, the liquid discharge valve is switched to be communicated with the liquid discharge needle, liquid suction is carried out while liquid injection is carried out, and liquid level uniformity is guaranteed.
During the standing process, the microplate 4 stands for t2 seconds, and the holding time t2 is fifteen seconds.
In the dehydration process, the times of patting the cleaning solution on the ELISA plate 4 into the waste liquid tank 31 and the times of patting the ELISA plate 4 into the wiping paper are three times.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical scope of the present invention by equally replacing or changing the technical idea of the present invention within the technical scope of the present invention.
Claims (10)
1. A plate washer for an ELISA plate is characterized by comprising:
the fixing part comprises a fixing seat and an outer shell arranged on the fixing seat, a single chip microcomputer control board is arranged in the outer shell, and a touch screen which is arranged on the outer shell and is communicated with the single chip microcomputer control board through serial port communication;
the cleaning part comprises an X-axis linear module arranged on the fixed seat and a first Z-axis linear module arranged on the X-axis linear module, and an ELISA plate to be cleaned is arranged on the first Z-axis linear module; the enzyme label plate cleaning device is characterized by further comprising a second Z-axis linear module arranged on the fixed seat, a cleaning pin row communicated with cleaning liquid is arranged on the second Z-axis linear module, the cleaning pin row is used for carrying out liquid injection cleaning on the enzyme label plate, and a pin row soaking groove for soaking the cleaning pin row is arranged on the fixed seat;
the dewatering part comprises an overturning oscillating part and a waste liquid groove which is arranged on the fixed seat and used for receiving the cleaning waste liquid of the ELISA plate; the rotary enzyme plate cleaning device is characterized by further comprising a cleaning part installed on the fixing seat, a liquid injection pump and a liquid discharge pump installed on the fixing seat, wherein the turnover oscillating part comprises a fixing box for installing the enzyme plate, an overturning part which is in transmission connection with the fixing box and can operate the fixing box to overturn, and an oscillating part which is connected with the overturning part and can drive the overturning part to reciprocate up and down to oscillate and dewater, the cleaning part comprises a paper supporting frame and is arranged on wiping paper on the paper supporting frame, and the rotary enzyme plate cleaning device further comprises a paper replacing component for moving and replacing the wiping paper.
2. The plate washer for microplate according to claim 1, characterized in that: the oscillating part comprises a first mounting block connected with the first Z-axis linear module and an oscillating motor mounted on the first mounting block; still include with the oscillating motor via the first transmission shaft of first shaft coupling transmission connection, and connect in the epaxial eccentric wheel of first transmission, still include one end connect in adjustable connecting rod on the eccentric wheel, the other end of adjustable connecting rod is connected with the second installation piece, install on the second installation piece upset portion, just the second installation piece spacing connect in on the first installation piece, with under the drive of adjustable connecting rod for reciprocating motion about the first installation piece.
3. The plate washer for microplate according to claim 2, characterized in that: the overturning part comprises an overturning motor arranged on the second mounting block and a second transmission shaft in transmission connection with the overturning motor through a second coupling, and the fixed box is arranged on the second transmission shaft.
4. The plate washer for microplate according to claim 2, characterized in that: a sliding rail is arranged on the first mounting block, and a sliding groove matched with the sliding rail is arranged on the second mounting block.
5. The plate washer for microplate according to claim 1, characterized in that: trade the paper subassembly adjacent in support paper frame sets up, it including install in mount pad on the fixing base, and install in stock form motor on the mount pad, still include with the stock form axle that stock form motor drive is connected, and adjacent in stock form axle and rotate connect in put the paper axle on the mount pad, in support and install on the paper frame and supply it is adjacent to wipe paper around a plurality of auxiliary shafts of establishing, on one of them auxiliary shaft of putting the paper axle installs paper range finding encoder.
6. The plate washer for microplate according to claim 5, characterized in that: the auxiliary shafts are four, and are a first auxiliary shaft, a second auxiliary shaft, a third auxiliary shaft and a fourth auxiliary shaft respectively, wherein the first auxiliary shaft, the second auxiliary shaft and the third auxiliary shaft are arranged at one end, close to the paper support, of the paper support, a triangular arrangement is formed, the fourth auxiliary shaft is arranged at the other end of the paper support, the winding path of the wiping paper is wound out from the side of the paper support, and the first auxiliary shaft, the second auxiliary shaft, the fourth auxiliary shaft and the third auxiliary shaft are sequentially wound into the paper winding shaft.
7. The plate washer for microplate according to claim 1, characterized in that: the fixed box comprises a U-shaped box frame and an end cover, wherein one end of the U-shaped box frame is hinged to the open end of the box frame through a hinge, and a magnetic attraction switch is arranged between the other end of the end cover and the box frame.
8. A microplate cleaning method using a microplate cleaning machine as defined in claim 1 to claim 7, characterized by comprising the steps of:
A. clicking a 'start' button on the touch screen, and performing plate washing process control according to a parameter value preset by a program of the singlechip control plate;
B. when the plate washing is started, carrying out an initialization process of a plate washing machine for the ELISA plate;
C. reading various state parameters of the current program scheme, including parameters such as cleaning time, cleaning times, cleaning rows, holding time, oscillation and slapping times and the like;
D. carrying out an initial oscillation slapping process on the ELISA plate before liquid injection cleaning;
E. moving the cleaning needle bank to the needle bank soaking groove for needle cleaning, and then performing a first cleaning and dehydrating process of the ELISA plate;
F. and judging whether to continue the secondary cleaning and dehydrating process according to the cleaning times parameter until the cleaning and dehydrating process is completely finished.
9. The microplate cleaning method according to claim 8, wherein in step D, the steps are specifically subdivided as follows:
d1, controlling a turnover part, turning over the ELISA plate, starting an oscillation part, and beating the residue in the ELISA plate to a waste liquid tank;
d2, controlling the X-axis linear module and the first Z-axis linear module to move the ELISA plate to the wiping paper, starting the oscillating part, and continuously shooting residues in the ELISA plate to the wiping paper;
d3, controlling the X-axis linear module and the first Z-axis linear module to move the ELISA plate to the plate washing position, and finishing the initial oscillation slapping process.
10. The microplate cleaning method according to claim 9, wherein in step E, the specific subdivision steps are as follows:
e1, needle washing process: controlling a second Z-axis linear module to drive a cleaning needle bar to reach a needle bar soaking groove position, starting a liquid injection pump, injecting cleaning liquid into the needle bar soaking groove through a liquid injection needle of the cleaning needle bar, simultaneously starting a liquid discharge pump, and discharging the cleaning liquid in the needle bar soaking groove through a liquid discharge needle of the cleaning needle bar, wherein the cleaning time is t1 seconds;
e2, liquid injection cleaning process: after the needle washing process is finished, controlling the washing needle to be arranged at the first arranging hole of the enzyme label plate, starting a liquid injection pump to inject washing liquid, starting a liquid discharge pump to lead the washing liquid to the liquid discharge needle, and sucking liquid while injecting the liquid so as to ensure that the liquid level is uniform; after the first row of holes is filled with liquid, judging whether to continue filling the next row of holes according to the cleaning row number until the last row of holes is filled with cleaning liquid;
e3, standing process: filling the cleaning solution into the rows of holes of all the enzyme label plates, and standing the enzyme label plates for t2 seconds;
e4, dehydration: after standing time is up, starting the oscillating part to drive the ELISA plate to perform oscillating and slapping actions, slapping cleaning liquid on the ELISA plate into a waste liquid tank, moving the ELISA plate to a wiping paper after slapping for multiple times, starting the oscillating part, performing oscillating and slapping actions again, slapping the ELISA plate onto the wiping paper, and enabling the wiping paper to suck residual liquid away;
e5, after the above processes are completed, the action parts are reset, and the first washing and dewatering process of the ELISA plate is finished.
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