KR100737508B1 - Automatic washing device of vessel in plant tissue culture - Google Patents

Abstract

An automatic washing device of vessels in plant tissue culture is provided to save working power, wash many vessels within a desired period, and improve washing convenience in the plant tissue culture process. The automatic washing device of culture vessels in plant tissue culture comprises: a turning motor having a rotation shaft(110); a plurality of guide members(210) binding to the rotation shaft and holding the culture vessels; a turntable(200) rotated by the turning motor; circular supporting member(300) located under the turntable and having a projected switch-binding portion(312) in the upper side; a body(600) which is located under the guide members of the turntable, supports the supporting member and contains a first washing tool for washing the vessels with water stored in the water tank, a second washing tool for washing the vessel with a brush, a third washing tool for washing the vessel with water, a drying tool for drying the washed vessel, and a volume type switch(500) for controlling the rotation speed of the turntable; and a console(700) which has a supporter(710) in one side of the body, and an operation button(750) for controlling the turning motor, first, second and third washing tool and drying tool and a LED(light emitted diode)(760) for displaying the operation status in one side of the supporter.

Description

Automatic washing device of vessel in plant tissue culture

1 is a perspective view showing an automatic plant tissue culture vessel washing device according to the present invention,

Figure 2 is a front view showing the plant tissue culture vessel automatic washing apparatus according to the present invention,

Figure 3 is a side view showing the plant tissue culture vessel automatic washing apparatus according to the present invention

4 is a plan view showing an automatic plant tissue culture vessel washing device according to the present invention,

5 is a plan view illustrating a state in which the culture vessel is fixed and operated on the guide member of the turntable according to the present invention;

Figure 6 is a schematic diagram showing the flow of detection and control signals between the control unit and each device of the automatic plant tissue culture vessel washing apparatus according to the present invention,

Figure 7 is a flow chart of the plant tissue culture vessel automatic washing apparatus according to the present invention.

<Description of Symbols for Major Parts of Drawings>

10: culture vessel

100: swing motor

110: rotation axis

200: turntable

210: guide member

300 support member

310: support piece 312: switch coupling part

314: limit switch

400: first cleaning means

402: water supply tank 404: first water supply pipe

404a: Pump 404b: First Solenoid Valve

406: first water spray nozzle

420: second washing means

422 brush 424 brush motor

426: brush lifting device 426a: cylinder

426b: Air solenoid 428: Position sensor

440: third washing means

442: second solenoid valve 444: second water supply pipe

446: second water spray nozzle

460: drying means

462: air compressor 464: air tank

466: supply pipe 466a: third solenoid valve

468: air spray nozzle 470: air nozzle lifting device

470a: Cylinder 470b: Air Solenoid

472: position sensor

500: volumetric switch

600: body

610 wheels

700 console

710: support 720: timer

730: relay switch 740: control unit

750: Operation Button 760: LED

The present invention relates to an automatic cleaning device for plant tissue culture vessels, and more specifically, various culture vessels such as flasks and Ringer's bottles used in plant tissue culture processes are fixed by a guide of a turntable, and then stopped after rotating at a predetermined angle. The present invention relates to a plant tissue culture vessel automatic cleaning device which is automatically washed through water washing, brush washing and drying.

Conventionally, the cleaning of plant tissue culture vessels has been mainly performed by an operator using a brush or the like by hand, which causes a problem that the washing precision decreases and the washing time of the culture vessel takes a lot.

In addition, the plant tissue culture medium (Broth) is used for the solid and liquid medium, in particular, since the remaining medium and residual residue after inoculation by washing and drying by manpower, there is a problem that takes manpower and time, In order to solve this problem, technology development of a system that can easily wash the culture vessel by drying with an air spray after wiping and rinsing with water washing and brush washing is required.

The present invention, in order to solve the conventional problems as described above, each of the six guide members are provided on the upper end of the turntable, each of the guide member supply unit, the first washing unit, the second washing unit, the third washing unit The purpose of the present invention is to provide a plant tissue culture container automatic washing device which is divided into a drying part and an outlet part to fix the culture air to the supply part, and then wash, rinse and dry in one system while the turntable rotates.

In order to achieve the above object, the automatic plant tissue culture vessel washing apparatus according to the present invention, in the apparatus for automatically washing various culture vessels, such as various flasks, Ringer's disease used in the plant tissue culture process, having a rotating shaft Swing motor; A turntable axially coupled to the rotating shaft and having a plurality of guide members configured to hold the culture vessel at regular intervals around an upper surface thereof, the turntable being rotated at a predetermined angle by the turning motor; A circular support member positioned below the turntable and having a switch coupling portion protruding upward; Located in the lower portion of the plurality of guide members of the turntable, the first washing means for washing the culture vessel with the wash water stored in the water supply tank, and the second washing for washing the culture vessel washed by the first washing means with a brush Means, third washing means for secondarily washing the culture vessel washed by the second washing means with washing water, drying means for drying the culture vessel washed by the third washing means, and a rotational speed of the turntable. A volume switch configured to control the body is provided therein and supports the support member; And a support provided at an upper end of one side of the main body, and an upper end of the support provided with an operating button for controlling the swing motor, first, second and third washing means, and a drying means and an LED displaying the same. Characterized in that comprises a.

In addition, the upper limit of the switch coupling portion of the support member, the limit switch for transmitting a detection signal to the console while the culture vessel is inserted into the guide member is rotated at a predetermined angle by the swing motor is contacted with the first, It is characterized in that the 2,3 washing means and the drying means are provided corresponding to each position installed.

In addition, the first washing means, the water supply tank in which the washing water is stored; A first water supply pipe having a pump for pumping the washing water stored in the water supply tank, the first water supply pipe having a first solenoid valve controlling a flow of the washing water pumped by the pump; And a first water spray nozzle connected to one end of the first water supply pipe and sprayed with water to cultivate the culture vessel. Characterized in that consists of.

In addition, the second washing means, a brush for cleaning the inside of the culture vessel; And a brush motor for rotating the brush. Characterized in that consists of.

In addition, the brush lifting device is provided with an air solenoid for operating the cylinder so that the brush is lifted; And a position sensor for sensing a lift position of the lifted cylinder. Characterized in that is provided.

In addition, the third washing means, the second water supply pipe is provided with a second solenoid valve for controlling the flow of the washing water; And a second water spray nozzle connected to one end of the second water supply pipe and sprayed with water to cultivate the culture vessel. Characterized in that consists of.

In addition, the drying means, the air compressor for compressing the outside air; An air tank in which air compressed by the air compressor is stored; An air supply pipe having a third solenoid valve for controlling air flow and connected to the air tank; And an air spray nozzle connected to one end of the air supply pipe and spraying air to the culture vessel for drying. Characterized in that consists of.

In addition, the air nozzle lifting apparatus is provided with an air solenoid for operating the cylinder so that the air spray nozzle is elevated; And a position sensor for sensing a lift position of the lifted cylinder. Characterized in that consists of.

The console may further include a timer for setting a time at which the turntable is rotated at a predetermined angle; And a relay switch for stopping the power supply of the swing motor when the timer expires. Characterized in that is provided.

Finally, the lower surface of the main body is characterized in that the moving wheel is provided to facilitate the movement.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.

1 is a perspective view showing an automatic plant tissue culture vessel washing device according to the present invention, Figure 2 is a front view showing an automatic plant tissue culture vessel washing device according to the present invention, Figure 3 is an automatic plant tissue culture vessel according to the present invention Figure 4 is a side view showing the washing device, Figure 4 is a plan view showing the automatic plant tissue culture vessel washing apparatus according to the present invention, Figure 5 is a plan view showing a state in which the operation of the culture vessel fixed to the guide member of the turntable according to the present invention. 6 is a system diagram showing the flow of detection and control signals between the control unit and each device of the automatic plant tissue culture vessel washing apparatus according to the present invention, Figure 7 is an operational flow chart of the automatic plant tissue culture vessel automatic washing apparatus according to the present invention to be.

As shown in Figures 1 to 7, the automatic plant tissue culture vessel washing apparatus according to the present invention, is provided with a rotating motor 100 having a rotating shaft 110, the rotating shaft 110 of the rotating motor 100 The central portion is axially coupled to the upper surface is provided with a plurality of guide members 210 for holding the culture vessel 10 at regular intervals, and the turntable 200 is rotated by a predetermined angle by the turning motor 100 do.

In the present invention, the guide member 210 is provided with six, respectively, the supply unit 1, the first washing unit 2, the second washing unit 3, the third washing unit 4, the drying unit 5 In addition, the discharge unit 6 performs a function.

On the other hand, it is located below the turntable 200, provided with a circular support member 300 is formed so that the switch coupling portion 312 protrudes to the top, the lower surface of the support member 300 will be described below A plurality of support pieces 310 are provided to be supported and fixed apart from the upper surface of the main body 600.

And, the upper end of the switch coupling portion 312 of the support member 300, the culture vessel 10 is inserted into the guide member 210 to be rotated at a predetermined angle by the swing motor 100 is in contact with Each position where the first, second and third washing means 400, 420, 440 and the drying means 460 are installed, the limit switch 314 transmitting a detection signal to the console 700 to be described below. It is provided to correspond to.

On the other hand, the guide member 210 of the turntable 200 is located in the counterclockwise direction, respectively, the first guide member 210 as the supply unit 1, the culture vessel 10 to be cleaned by inserting the fixed Let's go.

The second guide member 210 is the first washing unit 2, the culture vessel 10 fixed to the supply unit 1 is rotated at a predetermined angle by the rotation of the turntable 200, the first washing unit 2 If it is located at the top of the, it will be washed by spraying water. At this time, the first washing means 400 for washing by spraying water is provided with a water supply tank 402 for storing the washing water, and a pump 404a for pumping the washing water stored in the water supply tank 402 is provided. And a first water supply pipe 404 provided with a first solenoid valve 404b for controlling the flow of the washing water pumped by the pump 404a, and connected to one end of the first water supply pipe 404 to form a culture vessel. It consists of a first water spray nozzle 406 for washing by spraying water at 10 with water pressure.

The third guide member 210 is the second washing unit 3, the second washing unit while the culture vessel 10 washed in the first washing unit 2 is rotated at a predetermined angle by the rotation of the turntable 200 If it is located in the upper portion (3), using the brush 422 to clean the inside of the culture vessel (10). At this time, the second washing means 420 for washing the interior with the brush 422 is provided with a brush 422 for wiping the inside of the culture vessel 10, the brush motor for rotating the brush 422 ( 424 is provided, the brush lifting device 426 is provided with an air solenoid 426b for operating the cylinder 426a to raise and lower the brush 422, and the lifting position of the lifting cylinder 426a Position sensor 428 for detecting is provided.

In addition, the second washing means 420 is provided with a brush lifting device 426, thereby lifting up and down to remove the foreign matter adsorbed on the inner wall of the culture vessel (10).

The fourth guide member 210 is a third washing unit 4, the culture vessel 10 washed in the second washing unit 3 is rotated at a predetermined angle by the rotation of the turntable 200, the third washing unit If it is located at the top of (4), it is sprayed with water to wash. At this time, the third washing means 440 for washing by spraying water, the second water supply pipe 444 is provided with a second solenoid valve 446 for controlling the flow of the washing water, and the second water supply pipe 444 Is connected to one end of the culture vessel 10 is composed of a second water spray nozzle (446) for washing by spraying water with water pressure.

At this time, the other end of the second water supply pipe 444 is connected to the first water supply pipe 404 connected to the water supply tank 402. That is, the washing water stored in the water supply tank 402 is sent to the first and second water supply pipes 404 and 444 according to the operation of the pump 404a and flows under the control of the first and second solenoid valves 404b and 442. Will be determined.

The third washing means 440 is washed while rinsing the residue in the culture vessel 10 from which the foreign matter is removed by the brush 422 of the second washing means 420.

The fifth guide member 210 is a drying part 5, the male is generated in the process of washing the foreign matter adsorbed in the culture vessel (10) with a brush 422 washed in the third washing (4). The process of washing the residue with water) When the culture vessel 10 is positioned at the top of the drying unit 5 while rotating at a predetermined angle by the rotation of the turntable 200, the air is sprayed to dry. At this time, the drying means for spraying the air to dry 460, the air compressor 462 for compressing the outside air, and the air tank 464 to store the air compressed by the air compressor 462 and A third solenoid valve 466a for controlling the flow of air is provided and connected to the air supply pipe 466 connected to the air tank 464 and one end of the air supply pipe 466 to the culture vessel 10. It consists of an air spray nozzle 468 which injects air and dries.

At this time, the air nozzle lifting device 470 is provided with an air solenoid 470b for operating the cylinder 470a so that the air spray nozzle 468 is raised and lowered, the position for detecting the lifting position of the lifting cylinder (470a) The sensor 472 is configured.

The sixth guide member 210 is the discharge portion 6, the culture vessel 10 dried in the drying unit 5 is rotated at a predetermined angle by the rotation of the turntable 200, the upper portion of the discharge portion (6) When positioned in, it is possible to take out the culture vessel 10 is washed by washing, wiping, washing, drying process is complete.

On the other hand, the volume switch 500 for controlling the rotational speed of the turntable 200 is provided therein, the main body 600 for supporting the support member 300 is provided, the main body 600, one side To support the support 710 and the upper side of the support 710, the pivoting motor 100, the first, second and third washing means 400, 420, 440, drying means 460 is coupled to the upper end The console 700 is provided with an operation button 750 and an LED 760 for displaying the same, and a moving wheel 610 is provided to facilitate movement on the lower surface.

In this case, the console 700 includes a timer 720 for setting a time for each turn of the turntable 200 at a predetermined angle, and supplying power to the turning motor 100 when the timer 720 is driven out. A relay switch 730 for stopping is provided.

Looking at the operating state of the present invention plant tissue culture vessel automatic washing device with the configuration as described above with reference to FIG.

1) Turn on the power switch and the operation button (750).

2) Fix the culture vessel 10 to be washed to the guide member 210 of the supply unit 1 is fixed.

3) The control unit 740 rotates the turntable 200 counterclockwise at a predetermined angle by operating the turning motor 100 at a time defined by the timer 720. in)

4) The culture vessel 10 fitted to the guide member 210 moved to the first washing unit 2 comes in contact with the limit switch 314 of the first washing unit 2 and generates a detection signal in the console 700. The control unit 740 transmits.

5) The control unit 740 determines whether the culture vessel 10 is present in the first washing unit 2 and operates the first washing unit 400.

6) When the first washing means 400 is operated, the washing water stored in the water supply tank 402 is sucked into the pump 404a, passing through the first water supply pipe 404 and the opened first solenoid valve 404b, and the first water. It is injected into the culture vessel 10 through the injection nozzle 406. Meanwhile, when the guide member 210 in the empty state comes to the first washing unit 2 in 5, the control unit 740 has a first switch because the limit switch 314 of the first washing unit 2 is turned off. By operating the turning motor 100 directly without operating the cleaning means 400 is rotated counterclockwise at a predetermined angle. That is, it moves to the next 2nd washing part 3. In addition, the first solenoid valve 404b is initially closed, but is opened while the control unit 740 operates the first washing means 400.

7) The culture vessel 10 fitted to the guide member 210 moved to the second washing unit 3 transmits a detection signal to the controller 740 while turning on the limit switch 314 of the second washing unit 3. As a result, the control unit 740 operates the second washing means 420.

8) When the second washing means 420 is operated, the brush 422 is rotated while the brush motor 424 is operated. The brush 422 to rotate is wiped by the brush lifting device 426 installed on the lower side to wipe the inner wall of the culture vessel (10). On the other hand, when the guide member 210 in the empty state 7 comes to the second washing unit 3 in the above 7, because the limit switch 314 of the second washing unit 3 is turned off, the control unit 740 in the second By operating the turning motor 100 directly without operating the cleaning means 420 is rotated counterclockwise at a predetermined angle. That is, it is moved to the next third washing unit (4).

9) The culture vessel 10 fitted to the guide member 210 moved to the third washing unit 4 transmits a detection signal to the controller 740 while turning on the limit switch 314 of the third washing unit 4. As a result, the controller 740 operates the third washing means 440.

10) When the third washing means 440 is operated, the washing water introduced into the second water supply pipe 444 connected to the first water supply pipe 404 of the first washing means 400 is opened second solenoid valve 442. After passing through the second water spray nozzle 446 is washed by rinsing the residue in the culture vessel (10). On the other hand, when the guide member 210 in the empty state comes to the third washing unit 4 in the 9, the limit switch 314 of the third washing unit 4 is in the off state, the control unit 740 is the third By operating the turning motor 100 directly without operating the washing means 440 is rotated in a counterclockwise direction at a predetermined angle. That is, it moves to the next drying part 5. In addition, the second solenoid valve 442 is initially closed, but is opened while the control unit 740 operates the third washing means 440.

11) The culture vessel 10 fitted to the guide member 210 moved to the drying unit 5 transmits a detection signal to the control unit 740 while turning on the limit switch 314 of the drying unit 5, the control unit ( 740 causes the drying means 460 to operate.

12) When the drying means 460 is operated, the air compressed by the air compressor 462 is stored in the air tank 464, the stored air is opened through the air supply pipe 466, the third solenoid valve 466a After passing through the air spray nozzle 468 to dry the culture vessel (10). At this time, the air spray nozzle 468 is lifted up and down by the air nozzle lifting device 470, which is located from the upper portion after positioning the air spray nozzle 468 to the inside of the culture vessel (10) By spraying the water drops down will act as a drop. On the other hand, when the guide member 210 in the empty state in the 11 comes to the drying unit 5, the limit switch 314 of the drying unit 5 is turned off, so that the control unit 740 removes the drying means 440. By operating the turning motor 100 immediately without operation to rotate in a counterclockwise direction at a predetermined angle. That is, it is moved to the next discharge part (6). In addition, the third solenoid valve 466a is initially closed, but is opened while the controller 740 operates the drying means 460.

13) Take out the culture vessel 10 that has been washed and dried to the guide portion 210 moved to the discharge portion 6 is stored or used.

While the present invention has been illustrated and described with respect to preferred embodiments, the invention is not limited to the above-described embodiments, and is commonly used in the field of the invention without departing from the spirit of the invention as claimed in the claims. Anyone with a variety of variations will be possible.

According to the present invention described above, depending on the manpower to wash the culture vessel repeatedly made in the cultivation process of plant tissues, it is necessary to wash a large amount of culture vessel at the desired time as well as vitalization, so a lot of labor at a time Needed. Therefore, there is an effect that can greatly contribute to the vitalization of the plant tissue culture process by replacing a lot of labor as well as automating washing, rinsing, drying operations.

Claims (10)

In the apparatus for automatically washing a variety of culture vessels, such as various flasks, Ringer's disease used in the plant tissue culture process, A swing motor having a rotation axis; A turntable axially coupled to the rotating shaft and having a plurality of guide members configured to hold the culture vessel at regular intervals around an upper surface thereof, the turntable being rotated at a predetermined angle by the turning motor; A circular support member positioned below the turntable and having a switch coupling portion protruding upward; Located in the lower portion of the plurality of guide members of the turntable, the first washing means for washing the culture vessel with the wash water stored in the water supply tank, and the second washing for washing the culture vessel washed by the first washing means with a brush Means, third washing means for secondarily washing the culture vessel washed by the second washing means with washing water, drying means for drying the culture vessel washed by the third washing means, and a rotational speed of the turntable. A volume switch configured to control the body is provided therein and supports the support member; And A support is provided at an upper end of the main body, and an upper end of the support is provided with an operating button for controlling the swing motor, the first, second and third washing means, and a drying means and an LED displaying the same; Plant tissue culture vessel automatic washing apparatus comprising a. According to claim 1, wherein the upper end of the switch coupling portion of the support member, Each of the first, second, and third washing means and the drying means are provided with a limit switch which is inserted into the guide member and rotates at a predetermined angle by the turning motor to transmit a detection signal to the console. Automatic plant tissue culture vessel cleaning apparatus, characterized in that provided to correspond to the position. The method of claim 1, wherein the first washing means, A water tank in which washing water is stored; A first water supply pipe having a pump for pumping the washing water stored in the water supply tank, the first water supply pipe having a first solenoid valve controlling a flow of the washing water pumped by the pump; And A first water spray nozzle connected to one end of the first water supply pipe and sprayed with water to cultivate the culture vessel with water; Plant tissue culture vessel automatic cleaning device, characterized in that consisting of. The method of claim 1, wherein the second washing means, Brush for wiping the inside of the culture vessel; And A brush motor for rotating the brush; Plant tissue culture vessel automatic cleaning device, characterized in that consisting of. The method of claim 4, wherein Brush lift device provided with an air solenoid for operating the cylinder so that the brush is lifted; And A position sensor for sensing a lift position of the lifted cylinder; Plant tissue culture vessel automatic washing apparatus characterized in that it is provided. The method of claim 1, wherein the third washing means, A second water supply pipe having a second solenoid valve controlling a flow of washing water; And A second water spray nozzle connected to one end of the second water supply pipe and sprayed with water to cultivate the culture vessel; Plant tissue culture vessel automatic cleaning device, characterized in that consisting of. The method of claim 1, wherein the drying means, An air compressor for compressing outside air; An air tank in which air compressed by the air compressor is stored; An air supply pipe having a third solenoid valve for controlling air flow and connected to the air tank; And An air spray nozzle connected to one end of the air supply pipe and spraying air to the culture vessel for drying; Plant tissue culture vessel automatic cleaning device, characterized in that consisting of. The method of claim 7, wherein An air nozzle elevating device having an air solenoid for operating a cylinder to elevate the air spray nozzle; And A position sensor for sensing a lift position of the lifted cylinder; Plant tissue culture vessel automatic cleaning device, characterized in that consisting of. The method of claim 1, wherein the console, A timer for setting a time at which the turntable is rotated every constant angle; And A relay switch to stop supplying power to the turning motor when the timer expires; Plant tissue culture vessel automatic washing apparatus characterized in that it is provided. The method of claim 1, The plant tissue culture container automatic washing device, characterized in that the wheel is provided on the lower surface of the main body to facilitate movement.

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