CN107760561A - Nucleic acid-extracting apparatus and its application method - Google Patents
Nucleic acid-extracting apparatus and its application method Download PDFInfo
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- CN107760561A CN107760561A CN201711012247.5A CN201711012247A CN107760561A CN 107760561 A CN107760561 A CN 107760561A CN 201711012247 A CN201711012247 A CN 201711012247A CN 107760561 A CN107760561 A CN 107760561A
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- 238000000034 method Methods 0.000 title claims abstract description 8
- 239000007788 liquid Substances 0.000 claims abstract description 128
- 230000007246 mechanism Effects 0.000 claims abstract description 79
- 239000011324 bead Substances 0.000 claims abstract description 73
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 13
- 150000007523 nucleic acids Chemical class 0.000 claims description 12
- 102000039446 nucleic acids Human genes 0.000 claims description 12
- 108020004707 nucleic acids Proteins 0.000 claims description 12
- 230000005540 biological transmission Effects 0.000 description 7
- 230000004888 barrier function Effects 0.000 description 6
- 238000000605 extraction Methods 0.000 description 6
- 230000005693 optoelectronics Effects 0.000 description 5
- 239000000463 material Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 238000003491 array Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000004153 renaturation Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- -1 timber Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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Abstract
The invention provides a kind of nucleic acid-extracting apparatus and its application method, the nucleic acid-extracting apparatus includes frame, dropping liquid feed mechanism and Beads enrichment mechanism, also include the first driving group being connected with dropping liquid feed mechanism, the second driving group being connected with dropping liquid feed mechanism and the 3rd driving group being connected with Beads enrichment mechanism, the first driving group, the second driving group and the 3rd driving group to be connected with the frame.Nucleic acid-extracting apparatus provided by the invention, by setting the first driving group, the second driving group and the 3rd driving group so that dropping liquid feed mechanism and Beads enrichment mechanism may move, and avoid artificial mobile reagent box, improve the operating efficiency of the nucleic acid-extracting apparatus.
Description
Technical field
The invention belongs to technical field of medical equipment, is to be related to a kind of nucleic acid-extracting apparatus and its use more specifically
Method.
Background technology
With developing rapidly for medical field, there are more and more biological experiments based on nucleic acid, by right
Extraction, the detection of nucleic acid can make quick diagnosis to the disease of patient.At present in the device of extraction nucleic acid, for distributing reagent
Dropping liquid feed mechanism and the Beads enrichment mechanism for extracting nucleic acid it is separate, distribute reagent after need kit manually
It is transferred in Beads enrichment mechanism, causes the operating efficiency for extracting nucleic acid relatively low.
The content of the invention
It is an object of the invention to provide a kind of nucleic acid-extracting apparatus and its application method, to solve to exist in the prior art
Dropping liquid feed mechanism and Beads enrichment mechanism the problem of causing nucleic acid extraction efficiency low independently of each other.
To achieve the above object, the technical solution adopted by the present invention is：A kind of nucleic acid-extracting apparatus is provided, including frame,
Dropping liquid feed mechanism and Beads enrichment mechanism, the nucleic acid-extracting apparatus also include being connected and being used for the dropping liquid feed mechanism
The first driving group that it moves up in first level side is driven, is connected with the dropping liquid feed mechanism and for driving it the
The second driving group for being moved in two horizontal directions and it is connected with the Beads enrichment mechanism and for driving it horizontal the 3rd
The 3rd driving group just moved up, first driving group, second driving group and the 3rd driving group with it is described
Frame is fixedly connected.
Further, the first level direction and second horizontal direction are mutually perpendicular to, the first level direction
With the 3rd horizontal direction parallel.
Further, the dripping system includes multiple dropping liquid feed units, and the dropping liquid feed unit drives including dropping liquid
Dynamic component and the dropping liquid component for containing liquid, multiple dropping liquid components are in array distribution in the dropping liquid drive component
Above and with it is connected respectively.
Further, the dropping liquid feed unit also includes be used to limit the feed motion of the dropping liquid component spacing group
Part, the limit assembly are connected respectively with the dropping liquid drive component and the dropping liquid component.
Further, the limit assembly includes the first sliding block, elastic component and the institute being connected with the dropping liquid drive component
The second sliding block that the first sliding block is slidably connected is stated, and for limiting the limit switch of the elastic component decrement, it is described spacing
Switch is fixedly connected with first sliding block, and the both ends of the elastic component are consolidated with first sliding block and second sliding block respectively
Fixed connection, the dropping liquid component are fixedly connected with second sliding block.
Further, the Beads enrichment mechanism includes the kit of accommodating magnetic bead, can stretched into the kit and can
The bar magnet component of the magnetic bead is adsorbed, and is connected and with the bar magnet component for driving it to stretch into the bar magnet of the kit
Drive component.
Further, the Beads enrichment mechanism also includes being sheathed on the bar magnet component and can stretching into the kit
In bar magnet set, adsorbable bar magnet set magnetic sheet and be connected with the magnetic sheet and the magnetic for driving the magnetic sheet to move
Plate drive component, the bar magnet set is every between the bar magnet component and the kit.
Further, the magnetic sheet is provided with the first magnetic pole, and the bar magnet, which is put, to be provided with and can be adsorbed with first magnetic pole
The first Attraction block.
Further, the second magnetic pole for adsorbing the kit is additionally provided with the magnetic sheet, is set on the kit
There is the second Attraction block that can be adsorbed with second magnetic pole.
The present invention also provides a kind of application method of nucleic acid-extracting apparatus, comprises the following steps：
S10：Dropping liquid feed mechanism is moved to the top of the first kit by the first driving group and the second driving group, will
Reagent in dropping liquid feed mechanism is added in kit；
S20：Dropping liquid feed mechanism is moved to the top of the second kit by the first driving group and the second driving group；
S30：Beads enrichment mechanism is moved to the top of the first kit by the 3rd driving group；
S40：Beads enrichment mechanism is displaced downwardly in the first kit by bar magnet drive component and magnetic sheet drive component, and
Magnetic bead is adsorbed in Beads enrichment mechanism；
S50：Beads enrichment mechanism is moved to the top of the second kit by the 3rd driving group.
The beneficial effect of nucleic acid-extracting apparatus provided by the invention is：Compared with prior art, nucleic acid extraction of the present invention
Device is easy to try by setting the first driving group and the second driving group that dropping liquid feed mechanism can be moved to the top of kit
Agent is added in kit；Simultaneously by setting the 3rd driving group that Beads enrichment mechanism can be moved to the top of kit, just
Separated in by magnetic bead from reagent.In this way, dropping liquid feed mechanism and Beads enrichment mechanism can be moved respectively by multiple driving groups
To the top of kit, artificial mobile reagent box is avoided, improves the operating efficiency of nucleic acid extraction.
Brief description of the drawings
Technical scheme in order to illustrate the embodiments of the present invention more clearly, below will be to embodiment or description of the prior art
In the required accompanying drawing used be briefly described, it should be apparent that, drawings in the following description be only the present invention some
Embodiment, for those of ordinary skill in the art, without having to pay creative labor, can also be according to these
Accompanying drawing obtains other accompanying drawings.
Fig. 1 is the structure chart of nucleic acid-extracting apparatus provided in an embodiment of the present invention；
Fig. 2 be the embodiment of the present invention used by dropping liquid feed mechanism structure chart 1；
Fig. 3 be the embodiment of the present invention used by dropping liquid feed mechanism structure chart 2；
Fig. 4 is the installation diagram of limit assembly and feed tube used by the embodiment of the present invention；
Fig. 5 be the embodiment of the present invention used by limit assembly structure chart (the second sliding block is not shown)；
Fig. 6 be the embodiment of the present invention used by dropping liquid component structure chart；
Fig. 7 be the embodiment of the present invention used by Beads enrichment mechanism structure chart；
Fig. 8 be the embodiment of the present invention used by magnetic sheet structure chart；
Fig. 9 be the embodiment of the present invention used by bar magnet cover structure chart；
Figure 10 be the embodiment of the present invention used by kit structure chart；
Figure 11 be the embodiment of the present invention used by bar magnet component structure chart.
Wherein, each reference in figure：
1- dropping liquid feed mechanisms；10- dropping liquid feed units；11- dropping liquid drive components；111- dropping liquid drive divisions；112- drops
Liquid transmission component；1121- driving wheel groups；The belt pulleys of 1122- first；The belt pulleys of 1123- second；1124- belts；12- dropping liquid groups
Part；121- feed tubes；122- water droppers；13- limit assemblies；The sliding blocks of 131- first；1311- sliding panels；1312- slider bars；132-
Second sliding block；1321- accommodating cavities；1322- through holes；1323- light shielding parts；133- limit switches；1331- circuit boards；1332- feels
Light part；The photosensitive grooves of 13320-；134- elastic components；The guide rails of 135- second；The guide rails of 14- first；15- casings；151- sleeve pipes；2- magnetic beads
Separating mechanism；21- bar magnet drive components；22- bar magnet components；221- connecting plates；222- bar magnets；23- magnetic sheet drive components；
24- magnetic sheets；241- plate bodys；The magnetic poles of 242- second；The magnetic poles of 243- first；25- bar magnet sets；251- lids；2511- first is adsorbed
Block；2512- lug bosses；252- bodies；26- kits；260- solution cavities；The Attraction blocks of 261- second；3- frames；4- first drives
Dynamic group；The driving groups of 5- second；6 the 3rd driving groups.
Embodiment
In order that technical problems, technical solutions and advantages to be solved are more clearly understood, tie below
Drawings and Examples are closed, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only
To explain the present invention, it is not intended to limit the present invention.
It should be noted that when element is referred to as " being fixed on " or " being arranged at " another element, it can be directly another
On one element or it is connected on another element.When an element is known as " being connected to " another element, it can
To be directly to another element or be indirectly connected on another element.
It is to be appreciated that term " length ", " width ", " on ", " under ", "front", "rear", "left", "right", " vertical ",
The orientation or position relationship of the instruction such as " level ", " top ", " bottom " " interior ", " outer " are to be closed based on orientation shown in the drawings or position
System, it is for only for ease of and describes the present invention and simplify description, rather than indicates or imply that signified device or element must have
Specific orientation, with specific azimuth configuration and operation, therefore be not considered as limiting the invention.
In addition, term " first ", " second " are only used for describing purpose, and it is not intended that instruction or hint relative importance
Or the implicit quantity for indicating indicated technical characteristic.Thus, define " first ", the feature of " second " can be expressed or
Implicitly include one or more this feature.In the description of the invention, " multiple " are meant that two or more,
Unless otherwise specifically defined.
Referring to Fig. 1, existing illustrate to nucleic acid-extracting apparatus provided by the invention.The nucleic acid-extracting apparatus, including
Frame 3, dropping liquid feed mechanism 1, Beads enrichment mechanism 2, the first driving group 4, the second driving group 5 and the 3rd driving group 6.Dropping liquid enters
Connected to the driving group 5 of mechanism 1 second, the second driving group 5 and the first driving group 4 connect, the first driving group 4 driving dropping liquid feeding machine
Structure 1 moves up in first level side, and the second driving group 4 driving dropping liquid feed mechanism 1 moves in the second horizontal direction, the
Make dropping liquid feed mechanism 1 can be closer or far from kit 26 under the drive of one driving group 4 and the second driving group 5；3rd driving group
6 are connected with Beads enrichment mechanism 2, and the 3rd driving group 6 driving Beads enrichment mechanism 2 moves up in first level side, makes magnetic bead
Separating mechanism 2 can be closer or far from kit 26.
Nucleic acid-extracting apparatus provided by the invention, compared with prior art, nucleic acid-extracting apparatus of the present invention is by setting the
Dropping liquid feed mechanism 1 can be moved to the top of kit 26 by one driving group 4 and the second driving group 5, be easy to reagent being added to
In kit 26；Simultaneously by setting the 3rd driving group 6 that Beads enrichment mechanism 2 can be moved to the top of kit 26, it is easy to
Magnetic bead is separated from reagent.In this way, the nucleic acid-extracting apparatus can move dropping liquid feed mechanism 1 and magnetic bead by multiple driving groups
Separating mechanism 2, artificial mobile reagent box 26 is avoided, improve the operating efficiency of nucleic acid extraction.
Further, referring to Fig. 1, a kind of embodiment as nucleic acid-extracting apparatus provided by the invention, institute
State first level direction and second horizontal direction is mutually perpendicular to, the first level direction and the 3rd horizontal direction are put down
OK.Specifically, first level direction is the length direction of frame 3, and the second horizontal direction is the width of frame 3.
Further, Fig. 2 and Fig. 3 is referred to, a kind of specific embodiment party as nucleic acid-extracting apparatus provided by the invention
Formula, dropping liquid feed mechanism 1 include multiple dropping liquid feed units 10, and dropping liquid feed unit 10 includes dropping liquid drive component 11 and dropping liquid
Component 12, dropping liquid drive component 11 include providing the dropping liquid drive division 111 of power and the dropping liquid transmission component 112 of passing power,
Dropping liquid component 12 is used to contain liquid, and is squeezed the liquid in dropping liquid component 12 by devices such as the syringe of outside or air pumps
Go out.Dropping liquid drive component 11 is connected with dropping liquid component 12, and specifically, dropping liquid transmission component 112 is fixedly connected with dropping liquid component 12.
Dropping liquid drive division 111 and dropping liquid transmission component 112 in single dropping liquid feed unit 10 can control single dropping liquid component 12, institute
It can individually be controlled with each dropping liquid component 12 in the dropping liquid feed mechanism 1, lift its performance.
Further, Fig. 2 and Fig. 3 is referred to, a kind of specific embodiment party as nucleic acid-extracting apparatus provided by the invention
Formula, dropping liquid transmission component 112 include be connected with dropping liquid drive division 111 driving wheel group 1121, the first belt pulley 1122, the second skin
Belt wheel 1123 and belt 1124, belt 1124 are set around driving wheel group 1121, the first belt pulley 1122 and the second belt pulley
On 1123, and belt 1124 is fixedly connected with dropping liquid component 12.Specifically, dropping liquid drive division 111 can be motor, the output of motor
Axle is fixedly connected with driving wheel group 1121.
Further, Fig. 2 and Fig. 3 is referred to, a kind of specific embodiment party as nucleic acid-extracting apparatus provided by the invention
The external diameter of formula, the first belt pulley 1122 and the second belt pulley 1123 is equal, then the first belt pulley 1122 and the second belt pulley 1123
Gearratio be 1:1, transmission is more stable.The axial connecting line of first belt pulley 1122 and the second belt pulley 1123 and dropping liquid component
12 direction of feed is parallel to each other, and the direction of feed of dropping liquid component 12 is vertical direction, so the first belt pulley 1122 and second
The axial connecting line direction of belt pulley 1123 is also vertical direction.Belt 1124 is fixedly connected with dropping liquid component 12, so dropping liquid group
When part 12 in the axial connecting line direction of the first belt pulley 1122 and the second belt pulley 1123 is vertical direction, its feed motion is more
Steadily.Certainly, the external diameter of the first belt pulley 1122 and the second belt pulley 1123 also can be unequal, according to actually required gearratio
The external diameter of first belt pulley 1122 and the second belt pulley 1123 is set.
Further, Fig. 2 to Fig. 4 is referred to, a kind of specific embodiment party as nucleic acid-extracting apparatus provided by the invention
Formula, dropping liquid feed unit 10 also include limit assembly 13, and limit assembly 13 is used to limit dropping liquid component 12 in its direction of feed
Motion.Limit assembly 13 includes the first sliding block 131, elastic component 134, the second sliding block 132 and pressed for limiting elastic component 134
The limit switch 133 of contracting amount, the first sliding block 131 are fixedly connected with dropping liquid drive component 11, specially the first sliding block 131 and drop
Liquid drive division 111 is fixedly connected, and the second sliding block 132 is slidably connected with the first sliding block 131, and the both ends of elastic component 134 are respectively with
One sliding block 131 is fixedly connected with the second sliding block 132.Dropping liquid component 12 is fixedly connected with the second sliding block 132.When dropping liquid component 12
Descending elastic component 134 compresses when encountering barrier, and elastic component 134 triggers limit switch 133, limit after reaching certain decrement
Bit switch 133 sends electric signal and terminates the descending of the dropping liquid component 12, avoids the dropping liquid feed unit 10 from being extruded simultaneously by barrier
It is damaged to.
Further, Fig. 4 and Fig. 5 is referred to, a kind of specific embodiment party as nucleic acid-extracting apparatus provided by the invention
Formula, limit switch 133 are optoelectronic switch.Optoelectronic switch is using detected material blocking or reflect to light beam, connects circuit
The presence or absence of detection object.Specifically, in the dropping liquid feed unit 10, when optoelectronic switch is blocked by the second sliding block 132, then drip
Liquid component 12 runs into barrier when descending；When optoelectronic switch is not blocked by the second sliding block 132, dropping liquid component 12 then keeps normal
Feed motion.Response time of optoelectronic switch is short, high resolution and to the material less-restrictive of object to be detected, can detect modeling
Object made of the materials such as material, timber, metal.
Further, please continue to refer to Fig. 4 and Fig. 5, as a kind of specific real of nucleic acid-extracting apparatus provided by the invention
Mode is applied, limit switch 133 is provided with the photosensitive groove 13320 for being used for receiving light, and correspondingly, the second sliding block 132 is provided with can
The light shielding part 1323 moved in photosensitive groove 13320.Specifically, limit switch 133 includes what is be fixedly connected with the first sliding block 131
Circuit board 1331 and sensitive piece connected vertically with circuit board 1,331 1332, photosensitive groove 13320 are opened on sensitive piece 1332,
Photosensitive groove 13320 is groove.Light shielding part 1323 is convexly equipped on the second sliding block 132, and when dropping liquid component 12 encounters barrier, second slides
Block 132 is moved up relative to the first sliding block 131, and elastic component 134 is compressed, while light shielding part 1323 is also moved up to sense
In light groove 13320, the inwall of photosensitive groove 13320 is blocked, shielding signals are transmitted to dropping liquid drive division 111 by circuit board 1331,
Dropping liquid drive division 111 is set to be stopped, dropping liquid component 12 does not continue to descending.
Further, Fig. 2 and Fig. 3 is referred to, a kind of specific embodiment party as nucleic acid-extracting apparatus provided by the invention
Formula, dropping liquid feed unit 10 also include the guide rail 14 of casing 15 and first, and the first guide rail 14 is fixed on the both ends of casing 15, and dropping liquid drives
The side of casing 15 is fixed in dynamic portion 111.First guide rail 14 is slidably connected with the first sliding block 131.First sliding block 131 and dropping liquid group
Up and down motion of the part 12 under the drive of dropping liquid transmission component 112, under the support of the first guide rail 14, it is set to move up and down more flat
Surely, the accuracy of its stroke when carrying out feed motion of dropping liquid component 12 is improved.
Further, referring to Fig. 5, a kind of embodiment as nucleic acid-extracting apparatus provided by the invention, limit
Hyte part 13 also includes the second guide rail 135, and the second guide rail 135 is fixed on the first sliding block 131, and the second sliding block 132 and second is led
Rail 135 is slidably connected, i.e., the second sliding block 132 is slidably connected by the second guide rail 135 and the first sliding block 131.So in dropping liquid group
When part 12 runs into barrier, dropping liquid component 12 makes the second sliding block 132 be moved on the first sliding block 131 and triggers limit switch 133,
So that dropping liquid component 12 stop it is descending.Certainly, the first sliding block 131 and being slidably connected for the second sliding block 132 can also pass through it
He realizes mode, is not construed as limiting herein.
Further, Fig. 4 and Fig. 5 is referred to, a kind of specific embodiment party as nucleic acid-extracting apparatus provided by the invention
Formula, the first sliding block 131 include the sliding panel 1311 and slider bar 1312 being mutually permanently connected, the guide rail 14 of sliding panel 1311 and first
Length direction it is vertical, length direction of the slider bar 1312 along the first guide rail 14 is set, and sliding panel 1311 and slider bar 1312 are equal
It is slidably connected with the first guide rail 14.Specifically, sliding panel 1311 is fixedly connected with limit switch 133, slider bar 1312 and second
Sliding block 132 is slidably connected, and the first sliding block 131 and limit switch 133 are located at the top of the second sliding block 132, and the two of elastic component 134
End is individually fixed on the sliding block 132 of sliding panel 1311 and second, and elastic component 134 is vertically arranged.It is preferred that elastic component 134 is spring.
Certain first sliding block 131 and limit switch 133 can also be located at the left and right sides or front and rear sides of the second sliding block, can be according to specific
Position of the situation from the first sliding block 131 and limit switch 133.
Further, referring to Fig. 6, a kind of embodiment as nucleic acid-extracting apparatus provided by the invention, drop
Liquid component 12 includes feed tube 121 and water dropper 122.One end of feed tube 121 is fixedly connected with the second sliding block 132, is slided second
Moved up and down under the drive of block 132, the other end of feed tube 121 is connected with water dropper 122, and feed tube 122 is with feed tube 121 can
Dismantling connection is fixedly connected.Prevent dropping liquid component 12 from meeting other barriers when moving up and down, avoid influenceing feed tube 121
Feed liquor.Feed tube 121 is fixed on the second sliding block 132, is moved with the motion of the second sliding block 132.Specifically, casing 15
Lower end be additionally provided with the sleeve pipe 151 worn for feed tube 121, and the support block being fixedly connected with the first guide rail 14 is (in figure not
Show), after support block is fixedly connected with casing 15, and sleeve pipe 151 is fixedly connected with support block, and support block is located at sleeve pipe 151
Side, sleeve pipe 151 are used to protect feed tube 121.
Further, Fig. 4 to Fig. 5 is referred to, a kind of specific embodiment party as nucleic acid-extracting apparatus provided by the invention
Formula, the through hole 1322 that the second sliding block 132 is provided with the accommodating cavity 1321 of a side opening and worn for feed tube 123, accommodating cavity
1321 and through hole 1322 be interconnected, and feed tube 123 is stretched into accommodating cavity 1321.Specifically, the liquid feeding end of feed tube 123 is stretched
Enter in accommodating cavity 1321, its liquid feeding end can external air pump or syringe, the liquid in feed tube 123 is extruded into kit.
Accommodating cavity 1321 is opened up on second sliding block 132 and facilitates other the external feeding devices of feed tube 123, enters feeding device and dropping liquid
Separate device is separated into unit 10, mutual motion is unaffected.
Further, referring to Fig. 7, a kind of embodiment as nucleic acid-extracting apparatus provided by the invention, magnetic
Pearl separating mechanism 2, including kit 26, bar magnet component 22 and bar magnet drive component 21：Magnetic bead is contained in kit 26, magnetic bead is
Superparamagnetism silica nanometer magnetic bead, it can be combined on micro interface with nucleic acid molecules specific recognition and efficiently, kit 26
On offer solution cavity 260 for housing sample and magnetic bead；Bar magnet component 22 can stretch into kit 26 and fill sample and magnetic bead
Solution cavity 260 in, bar magnet component 22 has magnetic, can adsorb magnetic bead, so that magnetic bead be separated from sample；Bar magnet drives
Component 21 can drive bar magnet component 22 toward renaturation to stretch into or away from kit 26, when separating magnetic bead, stretch into kit 26,
When bar magnet component connection magnetic bead is taken out, away from kit 26.The Beads enrichment mechanism 2 also includes bar magnet set 25, the and of magnetic sheet 24
Magnetic sheet drive component 23：Bar magnet set 25 is sheathed on bar magnet component 22, and bar magnet set 25 is every located at bar magnet component 22 and kit 26
Between, when separating magnetic bead, bar magnet set 25 and bar magnet component 22 are stretched into kit 26, and bar magnet component 22 covers 25 across bar magnet
Magnetic bead is adsorbed on the surface of bar magnet set 25；24 adsorbable bar magnet of magnetic sheet set 25, it is easy to independent adsorption magnetic bar set 25, so as to just
In transfer magnetic bead and the bar magnet more renewed set 25；Magnetic sheet drive component 23 can drive magnetic sheet 24 to cover 25 closer or far from bar magnet, just
25 are covered in adsorbing and releasing bar magnet.
When using the nucleic acid-extracting apparatus, dropping liquid feed mechanism 1 is moved by the first driving group 4 and the second driving group 5
Move to the top of kit 26, dropping liquid component 12 is moved down and instills sample in kit 26, and dropping liquid feed mechanism 1 is moved to down
The top of one kit 26；The Beads enrichment mechanism 2 with bar magnet set 25 is moved to by the reagent by the 3rd driving group 6 again
The top of box 26, bar magnet set 25 are moved downward to the inside of kit 26, and magnetic bead is adsorbed in the outer wall of bar magnet set 25, Beads enrichment
Mechanism 2 is moved to elsewhere, and subsequent experimental operation is carried out to magnetic bead；After abandoning bar magnet set 25, Beads enrichment mechanism 2 is adsorbable
New bar magnet set 25.Repeat above step, you can quickly handle large batch of sample, and bar magnet component 22 need not be cleaned.
Further, Fig. 8 and Fig. 9 is referred to, a kind of specific embodiment party as nucleic acid-extracting apparatus provided by the invention
Formula, magnetic sheet 24 are provided with the first magnetic pole 243, and bar magnet set 25 is provided with the first Attraction block 2511, and the first magnetic pole 243 and first is inhaled
Can mutually it adsorb for attached piece 2511, i.e. magnetic sheet 24 and bar magnet set 25 can be adsorbed mutually.Under the drive of magnetic sheet drive component 23, magnetic
Rod set 25 can stretch into kit 26, so that magnetic bead is adsorbed in the surface of bar magnet set 25；Under the drive of magnetic sheet drive component 23,
Bar magnet set 25 can also be removed from kit 26, the magnetic bead for being easy to transfer to be adsorbed on 25 surfaces of bar magnet set, and magnetic bead is turned
Move in the kit used in subsequent step.
Further, Fig. 8 and Figure 10 is referred to, a kind of specific embodiment party as nucleic acid-extracting apparatus provided by the invention
Formula, the second magnetic pole 242 is additionally provided with magnetic sheet 24, kit 26 is provided with the second Attraction block 261, and the second magnetic pole 242 and second is inhaled
Can mutually it adsorb for attached piece 261, i.e. magnetic sheet 24 and kit 26 can be adsorbed mutually.Under the drive of magnetic sheet drive component 23, second
Magnetic pole 242 can move back and forth on the direction toward and away from kit 26, when the second magnetic pole 242 is close to the second Attraction block
When 261, the second magnetic pole 242 is powered, kit 26 can be picked up, be easy to transfering reagent box 26, consequently facilitating carrying out follow-up real
Test.First magnetic pole 243 and the second magnetic pole 242 are simultaneously operable so that magnetic sheet 24 simultaneously can inhale bar magnet set 25 and kit 26
Rise, be easy to bar magnet set 25 and kit 26 while be transferred to elsewhere.
Further, referring to Fig. 7, a kind of embodiment as nucleic acid-extracting apparatus provided by the invention, magnetic
Rod assembly 22 is that permanent magnet, the first magnetic pole 243 and the second magnetic pole 242 are electromagnet.Specifically, bar magnet component 22 stretches into bar magnet
After set 25, bar magnet component 22 and bar magnet set 25 are together stretched into kit 26, and bar magnet component 22 has magnetic and mutual with magnetic bead
Attract, and then magnetic bead is separated from sample, when bar magnet component 22 is away from bar magnet set 25, magnetic bead can cover on 25 from bar magnet and take off
Fall；First magnetic pole 243 and the first Attraction block 2511 are close to each other or when abutting against, and the first magnetic pole 243 is powered, the first magnetic pole
243 and bar magnet set 25 attract each other, bar magnet set 25 can follow moving up and down and moving for magnetic sheet 24, be easy to that magnetic bead will be adsorbed with
Bar magnet set 25 is taken out, and bar magnet set 25 can be put down after the power-off of the first magnetic pole 243；Second magnetic pole 242 and the second Attraction block 261 are mutual
It is close or when abutting against, the second magnetic pole 242 is powered, the second magnetic pole 242 and kit 26 attract each other, and kit 26 can follow
Magnetic sheet 24 is moved up and down and moved, kit 26 easy to remove, and kit 26 can be put down after the power-off of the second magnetic pole 242.
Further, referring to Fig. 8, a kind of embodiment as nucleic acid-extracting apparatus provided by the invention, magnetic
Plate 24 includes plate body 241, and bar magnet component 22 is arranged on plate body 241, and multiple holes passed through for bar magnet component 22 are offered on plate,
Plate body 241 is connected with magnetic sheet drive component 23, and magnetic sheet 24 moves up and down under the drive of drive component, the He of the first magnetic pole 243
Second magnetic pole 242 is fixedly connected with plate body 241.Specifically, the first magnetic pole 243 and the second magnetic pole 242 are located at plate body 241
Homonymy.The quantity of first magnetic pole 243 and the second magnetic pole 242 can be multiple, and preferably the first magnetic pole 243 and the second magnetic pole 242 are all provided with
At the corner of the side of plate body 241.
Further, referring to Fig. 9, a kind of embodiment as nucleic acid-extracting apparatus provided by the invention, magnetic
Rod set 25 includes lid 251 and multiple bodies 252, and lid 251 and kit 26 are parallel to each other, can be placed on kit 26, keep away
The sample exempted from kit 26 is contaminated in experimentation, and the first Attraction block 2511 is on lid 251.Multiple bodies
252 arrays can stretch into kit 26 in the side of lid 251, body 252 under the drive of magnetic sheet 24, and move up and down and make
Nucleic acid separates.The quantity of body 252, kit 26 house the solution cavity 260 of sample quantity and bar magnet 222 quantity it is equal,
The quantity of above-mentioned all parts is determined according to actual conditions, is not construed as limiting herein.
Further, referring to Fig. 9, a kind of embodiment as nucleic acid-extracting apparatus provided by the invention, set
Body 252 has bar magnet chamber, a side opening of bar magnet chamber, bar magnet component 22 is stretched into from the opening in body 252.In separation magnetic bead
When, each bar magnet 222 of bar magnet component 22 is located at bar magnet intracavitary, and bar magnet chamber is located in kit 26, and magnetic bead is attracted to body
252 outside, sample contamination and the outside of body 252, without being infected with bar magnet component 22, separating the magnetic of next sample
During pearl, bar magnet set 25 can be directly changed, without cleaning bar magnet component 22, substantially increases the separation effect of the Beads enrichment mechanism 2
Rate.
Further, Figure 11 is referred to, as a kind of embodiment of nucleic acid-extracting apparatus provided by the invention, magnetic
Rod assembly 22 includes connecting plate 221 and multiple bar magnets 222.Connecting plate 221 and bar magnet drive component 21 connect, in bar magnet driving group
Moved up and down under the drive of part 21, bar magnet 222 is stretched into or away from kit 26.Multiple arrays of bar magnet 222 are in connecting plate 221
Side, and can be stretched under the drive of connecting plate 221 in bar magnet chamber.
Further, referring to Fig. 9, a kind of embodiment as nucleic acid-extracting apparatus provided by the invention, lid
Body 251 is provided with the lug boss 2512 that can be abutted against with kit 26.The quantity of lug boss 2512 can be multiple, at least be located at
The opposite sides of lid 251, make that there is spacing between lid 251 and kit 26.Stretch into kit 26 and divide in body 252
After magnetic bead, bar magnet need to be covered 25 and be taken out from kit 26, the setting of lug boss 2512 causes the He of lid 251 of bar magnet set 25
Kit 26 will not be inter-adhesive in the presence of atmospheric pressure, is easy to take out bar magnet set 25.
The present invention also provides a kind of application method of nucleic acid-extracting apparatus, comprises the following steps：
S10：Dropping liquid feed mechanism 1 is moved to by the upper of the first kit 26 by the first driving group 4 and the second driving group 5
Side, the reagent in dropping liquid feed mechanism 1 is added in kit 26；
S20：Dropping liquid feed mechanism 1 is moved to by the upper of the second kit 26 by the first driving group 4 and the second driving group 5
Side；
S30：Beads enrichment mechanism 2 is moved to the top of the first kit 26 by the 3rd driving group 6；
S40：Beads enrichment mechanism 2 is displaced downwardly to by the first kit by bar magnet drive component 21 and magnetic sheet drive component 23
In 26, and magnetic bead is adsorbed in Beads enrichment mechanism 2；
S50：Beads enrichment mechanism 2 is moved to the top of the second kit 26 by the 3rd driving group 6.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should be included in the scope of the protection.
Claims (10)
1. nucleic acid-extracting apparatus, it is characterised in that：Including frame, dropping liquid feed mechanism and Beads enrichment mechanism, the nucleic acid carries
Take device also to include being connected with the dropping liquid feed mechanism and be used for the first driving for driving it to be moved up in first level side
Group, the second driving group for driving it to be moved in the second horizontal direction, Yi Jiyu are connected and are used for the dropping liquid feed mechanism
The Beads enrichment mechanism connects and for driving the 3rd driving group that it is moved in the 3rd horizontal direction, first driving
Group, second driving group and the 3rd driving group are connected with the frame.
2. nucleic acid-extracting apparatus as claimed in claim 1, it is characterised in that：The first level direction and second level
Direction is mutually perpendicular to, the first level direction and the 3rd horizontal direction parallel.
3. nucleic acid-extracting apparatus as claimed in claim 1, it is characterised in that：The dropping liquid feed mechanism enters including multiple dropping liquids
To unit, the dropping liquid feed unit includes dropping liquid drive component and the dropping liquid component for containing liquid, multiple drops
Liquid component is connected respectively in array distribution on the dropping liquid drive component and with it.
4. nucleic acid-extracting apparatus as claimed in claim 3, it is characterised in that：The dropping liquid feed unit also includes being used to limit
The limit assembly of the feed motion of the dropping liquid component, the limit assembly and the dropping liquid drive component and the dropping liquid component
Connect respectively.
5. nucleic acid-extracting apparatus as claimed in claim 4, it is characterised in that：The limit assembly includes driving with the dropping liquid
The first sliding block, elastic component, the second sliding block being slidably connected with first sliding block of component connection, and for limiting the bullet
Property part decrement limit switch, the limit switch is fixedly connected with first sliding block, the both ends difference of the elastic component
It is fixedly connected with first sliding block and second sliding block, the dropping liquid component is fixedly connected with second sliding block.
6. nucleic acid-extracting apparatus as claimed in claim 1, it is characterised in that：The Beads enrichment mechanism includes accommodating magnetic bead
Kit, can stretch into the kit and the bar magnet component of the adsorbable magnetic bead, and be connected with the bar magnet component and
For driving it to stretch into the bar magnet drive component of the kit.
7. nucleic acid-extracting apparatus as claimed in claim 6, it is characterised in that：The Beads enrichment mechanism also includes being sheathed on institute
The magnetic sheet of the bar magnet set stating on bar magnet component and can stretch into the kit, the adsorbable bar magnet set, and with the magnetic
Plate connects and the magnetic sheet drive component for driving it to move, and the bar magnet set is every located at the bar magnet component and the kit
Between.
8. nucleic acid-extracting apparatus as claimed in claim 7, it is characterised in that：The magnetic sheet is provided with the first magnetic pole, the magnetic
Rod is put provided with the first Attraction block that can be adsorbed with first magnetic pole.
9. nucleic acid-extracting apparatus as claimed in claim 8, it is characterised in that：The second magnetic pole is additionally provided with the magnetic sheet, it is described
Kit is provided with the second Attraction block that can be adsorbed with second magnetic pole.
10. the application method of nucleic acid-extracting apparatus, it is characterised in that comprise the following steps：
S10：Dropping liquid feed mechanism is moved to the top of the first kit by the first driving group and the second driving group, by dropping liquid
Reagent in feed mechanism is added in kit；
S20：Dropping liquid feed mechanism is moved to the top of the second kit by the first driving group and the second driving group；
S30：Beads enrichment mechanism is moved to the top of the first kit by the 3rd driving group；
S40：Beads enrichment mechanism is displaced downwardly in the first kit by bar magnet drive component and magnetic sheet drive component, and by magnetic
Pearl is adsorbed in Beads enrichment mechanism；
S50：Beads enrichment mechanism is moved to the top of the second kit by the 3rd driving group.
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| CN201711012247.5A CN107760561B (en) | 2017-10-26 | 2017-10-26 | Nucleic acid-extracting apparatus and method for extracting nucleic acid |
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| CN201711012247.5A CN107760561B (en) | 2017-10-26 | 2017-10-26 | Nucleic acid-extracting apparatus and method for extracting nucleic acid |
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| CN108277148A (en) * | 2018-04-25 | 2018-07-13 | 江苏硕世生物科技股份有限公司 | Nucleic acid extraction liquid distributing device |
| CN108410859A (en) * | 2018-04-28 | 2018-08-17 | 绍兴迅敏康生物科技有限公司 | Method for extracting nucleic acid |
| CN108774619A (en) * | 2018-06-26 | 2018-11-09 | 杭州优思达生物技术有限公司 | Without alcoholization nucleic acid detection reagent pipe |
| CN111218398A (en) * | 2020-03-25 | 2020-06-02 | 广州高盛生物科技股份有限公司 | Magnetic bead extraction module and extraction method |
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| CN108410859A (en) * | 2018-04-28 | 2018-08-17 | 绍兴迅敏康生物科技有限公司 | Method for extracting nucleic acid |
| CN108774619A (en) * | 2018-06-26 | 2018-11-09 | 杭州优思达生物技术有限公司 | Without alcoholization nucleic acid detection reagent pipe |
| CN111218398A (en) * | 2020-03-25 | 2020-06-02 | 广州高盛生物科技股份有限公司 | Magnetic bead extraction module and extraction method |
| CN111218398B (en) * | 2020-03-25 | 2024-02-13 | 广州高盛生物科技股份有限公司 | Magnetic bead extraction module and extraction method |
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|---|---|
| CN107760561B (en) | 2019-05-28 |
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