CN216192372U

CN216192372U - Magnetic bead method solid-liquid separation equipment

Info

Publication number: CN216192372U
Application number: CN202122044626.0U
Authority: CN
Inventors: 杨静
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-08-27
Filing date: 2021-08-27
Publication date: 2022-04-05
Anticipated expiration: 2031-08-27

Abstract

The utility model discloses a magnetic bead method solid-liquid separation device which comprises a base, wherein four groups of supporting legs are arranged at the lower end of the base, a device rack is fixedly connected to the upper end of the base, a separation part is detachably mounted at the central position of the upper end of the base, a centrifugal device is arranged inside the separation part, a separation device is arranged at one end of the separation part, a driving mechanism is mounted on the inner wall of the device rack, the separation device comprises a separation magnet which is attached to one end of the separation part, and a separation magnet is fixedly connected to one end of a separation moving block. The magnetic bead method solid-liquid separation device has the advantages that the automation degree of a magnetic bead taking mode is high, and the magnetic bead method solid-liquid separation device is practical.

Description

Magnetic bead method solid-liquid separation equipment

Technical Field

The utility model relates to the technical field of propellers, in particular to a magnetic bead method solid-liquid separation device.

Background

With the popularization of gene detection, personalized drug delivery, prenatal diagnosis and the like, high-throughput and automation are pursued in various fields of the biological industry today, the limitation of the traditional DNA extraction method is more and more obvious, and DNA/RNA in cells or tissues is released under the action of lysate. At this time, the surface-modified superparamagnetic silica nano magnetic beads are specifically combined with nucleic acid to form a nucleic acid-magnetic bead compound. Then under the action of external magnetic field, the complex is separated out. Removing non-specifically adsorbed impurities by using a washing solution, and finally dissociating the nucleic acid adsorbed on the surface of the magnetic beads by using a specific eluent to obtain the extracted target nucleic acid substance. The former separating device has poor effect when in use, low degree of automation and inconvenient use.

SUMMERY OF THE UTILITY MODEL

The utility model mainly aims to provide a magnetic bead method solid-liquid separation device which can effectively solve the problems in the background technology.

In order to achieve the purpose, the utility model adopts the technical scheme that:

the utility model provides a magnetic bead method solid-liquid separation equipment, includes the base, the lower extreme of base is provided with four groups of supporting legss, the upper end fixedly connected with device frame of base, the upper end central point of base puts demountable installation and has the separation portion, the inside of separation portion is provided with centrifugal device, the one end of separation portion is provided with separator, actuating mechanism is installed to the inner wall of device frame, separator sets up the separation magnet in the one end of separation portion including the laminating, the one end fixedly connected with separation magnet of separation movable block.

Preferably, the separation part is including setting up the knockout drum that puts at the upside central point of base, be connected with the stabilizer blade between knockout drum and the base, the inside of stabilizer blade is run through and is provided with the location knob, fixed connection between stabilizer blade and the knockout drum, the location knob runs through the inside of stabilizer blade, threaded connection between stabilizer blade and the base.

Preferably, the driving mechanism comprises a first track arranged on the inner wall of one side of the device rack, a first motor is fixedly mounted at the upper end of the device rack close to the upper side of the first track, a first lead screw penetrates through the inside of the first track, the first lead screw is movably connected with the first track, and the separation moving block is in threaded connection with the first lead screw.

Preferably, a first belt pulley is arranged at the lower side of the base at the outer side of the first screw rod, a second rail is arranged at the inner wall of the other side of the device rack, a second screw rod penetrates through the inside of the second rail, and the second screw rod is movably connected with the second rail.

Preferably, a second belt pulley is arranged at the position, close to the lower side of the base, of the outer side of the second screw rod, and a belt is arranged between the second belt pulley and the first belt pulley.

Preferably, the centrifugal device comprises a centrifugal moving block in threaded connection with the outer side of the second lead screw, a motor mounting plate is arranged at one end of the centrifugal moving block, a motor is arranged at the upper end of the motor mounting plate, a rotating shaft is connected to an output shaft at the lower end of the motor, and a stirring blade is fixedly connected to the outer side of the rotating shaft.

Compared with the prior art, the utility model has the following beneficial effects:

1. the stirring blade can stir the mixed liquid in the separating tank through the rotation of the rotating shaft, so that the centrifugal effect is achieved, the nucleic acid-magnetic bead compound is not accumulated at the bottom of the separating tank, and the effect of magnetic bead separation in the next step is better.

2. The second belt pulley can be rotated through belt transmission, so that the purpose of synchronous rotation between the second screw rod fixedly connected with the second belt pulley and the first screw rod is achieved, linkage can be achieved, and the belt transmission device is practical.

3. The purpose of separating the nucleic acid-magnetic bead compound from the solution in the separation tank is achieved, the automation degree is high, and the method is practical.

Drawings

FIG. 1 is a schematic diagram of the overall structure of a magnetic bead method solid-liquid separation device according to the present invention;

FIG. 2 is a schematic diagram of the overall structure of a magnetic bead method solid-liquid separation device according to the present invention;

FIG. 3 is a front view of a magnetic bead method solid-liquid separation apparatus according to the present invention;

FIG. 4 is a schematic view of a partial structure of a magnetic bead method solid-liquid separation apparatus according to the present invention;

FIG. 5 is a schematic diagram showing the structure of the separation section of the magnetic bead method solid-liquid separation apparatus according to the present invention.

In the figure: 1. a base; 2. supporting legs; 3. a device frame; 4. a separation section; 41. a separation tank; 42. a support leg; 43. positioning a knob; 5. a centrifugal device; 51. a centrifugal moving block; 52. a motor mounting plate; 53. a motor; 54. a rotating shaft; 55. stirring blades; 6. a separation device; 61. separating the moving block; 62. separating the magnet; 7. a drive mechanism; 71. a first track; 72. a first motor; 73. a first lead screw; 74. a first pulley; 75. a second track; 76. a second lead screw; 77. a second pulley; 78. a belt.

Detailed Description

In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the utility model easy to understand, the utility model is further described with the specific embodiments.

As shown in fig. 1 to 5, a magnetic bead method solid-liquid separation device comprises a base 1, four groups of supporting legs 2 are arranged at the lower end of the base 1, a device frame 3 is fixedly connected to the upper end of the base 1, a separation part 4 is detachably mounted at the center position of the upper end of the base 1, a centrifugal device 5 is arranged inside the separation part 4, a separation device 6 is arranged at one end of the separation part 4, a driving mechanism 7 is mounted on the inner wall of the device frame 3, the separation device 6 comprises a separation magnet 62 attached to one end of the separation part 4, and a separation magnet 62 is fixedly connected to one end of a separation moving block 61.

The separating part 4 comprises a separating tank 41 arranged at the center of the upper side of the base 1, a supporting leg 42 is connected between the separating tank 41 and the base 1, a positioning knob 43 penetrates through the inside of the supporting leg 42, the supporting leg 42 is fixedly connected with the separating tank 41, the positioning knob 43 penetrates through the inside of the supporting leg 42, and the supporting leg 42 is in threaded connection with the base 1.

The support legs 42 can be conveniently separated from the base 1 by screwing the positioning knob 43, so that the aim of conveniently dismantling the separating tank 41 for cleaning is fulfilled.

The driving mechanism 7 comprises a first track 71 arranged on the inner wall of one side of the device rack 3, a first motor 72 is fixedly mounted at the upper end of the device rack 3 close to the upper side of the first track 71, a first lead screw 73 is arranged inside the first track 71 in a penetrating manner, the first lead screw 73 is movably connected with the first track 71, a separation moving block 61 is in threaded connection with the first lead screw 73, a first belt pulley 74 is arranged at the lower side of the outer side base 1 of the first lead screw 73, a second track 75 is arranged on the inner wall of the other side of the device rack 3, a second lead screw 76 is arranged inside the second track 75 in a penetrating manner, the second lead screw 76 is movably connected with the second track 75, a second belt pulley 77 is arranged at the outer side of the second lead screw 76 close to the lower side of the base 1, and a belt 78 is arranged between the second belt pulley 77 and the first belt pulley 74.

When the separation is carried out, the first motor 72 is started, so that the first screw rod 73 is driven to rotate, the first belt pulley 74 fixedly connected with the first screw rod 73 can rotate through the rotation of the first screw rod 73, the first belt pulley 74 can drive the belt 78 to transmit through the rotation of the first belt pulley 74, the second belt pulley 77 can rotate through the transmission of the belt 78, the purpose of synchronous rotation between the second screw rod 76 fixedly connected with the second belt pulley 77 and the first screw rod 73 is achieved, linkage can be realized, and the separation device is practical.

The centrifugal device 5 comprises a centrifugal moving block 51 which is in threaded connection with the outer side of the second lead screw 76, a motor mounting plate 52 is arranged at one end of the centrifugal moving block 51, a motor 53 is arranged at the upper end of the motor mounting plate 52, a rotating shaft 54 is connected to the lower end output shaft of the motor 53, and a stirring blade 55 is fixedly connected to the outer side of the rotating shaft 54.

When the separating tank 41 is used, the mixture is placed in the separating tank 41, the rotating shaft 54 can be rotated by starting the motor 53, and the stirring blades 55 can be stirred in the mixed liquid in the separating tank 41 by rotating the rotating shaft 54, so that the centrifugal effect is achieved, the nucleic acid-magnetic bead compound is not accumulated at the bottom of the separating tank 41, and the magnetic bead separation effect in the next step can be better performed.

The working principle is as follows:

when the nucleic acid-magnetic bead separation device is used, the mixture is placed in the separation tank 41, the rotating shaft 54 can be rotated by starting the motor 53, and the stirring blades 55 can be stirred in the mixed liquid in the separation tank 41 by rotating the rotating shaft 54, so that the centrifugal effect is achieved, the nucleic acid-magnetic bead compound is prevented from being accumulated at the bottom of the separation tank 41, and the magnetic bead separation effect in the next step can be better performed;

when the separation is performed, the first motor 72 is started, so that the first screw rod 73 is driven to rotate, the first belt pulley 74 fixedly connected with the first screw rod 73 can rotate through the rotation of the first screw rod 73, the first belt pulley 74 can drive the belt 78 to transmit through the rotation of the first belt pulley 74, and the second belt pulley 77 can rotate through the transmission of the belt 78, so that the purpose of synchronous rotation between the first screw rod 73 and the second screw rod 76 fixedly connected with the second belt pulley 77 is achieved, the linkage can be realized, and the separation is practical;

when the separation of the nucleic acid-magnetic bead complex is performed, the centrifugal device 5 is moved upward by the rotation of the second lead screw 76, so that the stirring blade 55 and the rotating shaft 54 are taken out from the inside of the separation tank 41, thereby preventing the centrifugal device 5 from affecting the operation of the separation device 6, and when the centrifugal device 5 is moved upward, the first lead screw 73 drives the separation moving block 61 to move upward, the separation moving block 61 is moved upward to drive the separation magnet 62 to slide upward along the outer wall of the separation tank 41, so that the nucleic acid-magnetic bead complex is adsorbed by the separation magnet 62 and attached to the inner wall of the separation tank 41, and along with the upward movement of the separation magnet 62, the nucleic acid-magnetic bead complex is also moved upward along with the separation magnet 62, until when the separation magnet 62 is separated from the separation tank 41, the adsorbed nucleic acid-magnetic bead complex is adsorbed to the separation magnet 62, thereby, the purpose of separating the nucleic acid-magnetic bead complex from the solution in the separation tank 41 is achieved, and the method of taking out the magnetic beads is highly automated and is relatively practical.

The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the utility model as claimed. The scope of the utility model is defined by the appended claims and equivalents thereof.

Claims

1. The utility model provides a magnetic bead method solid-liquid separation equipment, includes base (1), its characterized in that: the lower extreme of base (1) is provided with four groups of supporting legs (2), the upper end fixedly connected with device frame (3) of base (1), the upper end central point of base (1) puts demountable installation and has separation portion (4), the inside of separation portion (4) is provided with centrifugal device (5), the one end of separation portion (4) is provided with separator (6), actuating mechanism (7) are installed to the inner wall of device frame (3), separator (6) set up separation magnet (62) in the one end of separation portion (4) including the laminating, the fixed separation movable block (61) that is provided with of one end of separation magnet (62).

2. The magnetic bead method solid-liquid separation device according to claim 1, characterized in that: separation portion (4) are including setting up knockout drum (41) of the upside central point of base (1) and putting, be connected with stabilizer blade (42) between knockout drum (41) and base (1), the inside of stabilizer blade (42) is run through and is provided with location knob (43), fixed connection between stabilizer blade (42) and knockout drum (41), the inside of stabilizer blade (42) is run through in location knob (43), threaded connection between stabilizer blade (42) and base (1).

3. The magnetic bead method solid-liquid separation device according to claim 1, characterized in that: the driving mechanism (7) comprises a first track (71) arranged on the inner wall of one side of the device rack (3), a first motor (72) is fixedly mounted at the upper end of the device rack (3) close to the upper side of the first track (71), a first screw rod (73) penetrates through the inside of the first track (71), the first screw rod (73) is movably connected with the first track (71), and the separation moving block (61) is in threaded connection with the first screw rod (73).

4. The magnetic bead method solid-liquid separation device according to claim 3, characterized in that: the device is characterized in that a first belt pulley (74) is arranged at the lower side of the outer side base (1) of the first screw rod (73), a second rail (75) is arranged at the inner wall of the other side of the device rack (3), a second screw rod (76) penetrates through the inner portion of the second rail (75), and the second screw rod (76) is movably connected with the second rail (75).

5. The magnetic bead method solid-liquid separation device according to claim 4, characterized in that: the outside of second lead screw (76) is close to the downside department of base (1) and is provided with second belt pulley (77), be provided with belt (78) between second belt pulley (77) and first belt pulley (74).

6. The magnetic bead method solid-liquid separation device according to claim 5, characterized in that: the centrifugal device (5) comprises a centrifugal moving block (51) which is in threaded connection with the outer side of the second lead screw (76), a motor mounting plate (52) is arranged at one end of the centrifugal moving block (51), a motor (53) is arranged at the upper end of the motor mounting plate (52), a rotating shaft (54) is connected to the lower end output shaft of the motor (53), and a stirring blade (55) is fixedly connected to the outer side of the rotating shaft (54).