TWI828389B - Modules for processing samples and biological sample processing apparatuses comprising the same - Google Patents

Abstract

Disclosed herein is a module for processing samples. The module comprises a first lifting panel, a fixing panel, a threaded nipple, and a first actuator. According to the embodiments of the present disclosure, the first lifting panel is coupled with the first actuator and the threaded nipple, which is further screwed with the fixing panel to rotate the threaded nipple while lifting.

Description

Sample processing module and biological sample processing equipment including the sample processing module Prepare

The present invention relates to a sample processing module, particularly a sample processing module suitable for automated biological sample processing equipment.

Extraction of nucleic acids (such as deoxyribonucleic acid (DNA) or ribonucleic acid (RNA)) is a primary step in conducting various studies such as molecular biology, genetics, medicine, and epidemiology, as well as clinical diagnosis. As nucleic acid extraction technology becomes more mature, there are many extraction kits and automated extraction equipment on the market to achieve rapid and large-scale extraction of nucleic acids.

In recent years, automated nucleic acid extraction equipment has been constantly innovating, especially automated nucleic acid extraction equipment using magnetic bead technology platforms. Generally speaking, the current device includes three sets of driving devices, which are used to drive the magnetic component to move, drive the casing to move, and drive the casing to rotate. The magnetic component is controlled to capture magnetic beads through magnetic force, and through The cannula handles the sample and stirs it. Therefore, the automated nucleic acid extraction equipment in the previous technology is larger in size and requires a larger space to place the equipment, resulting in certain restrictions on usage conditions.

In view of this, in order to improve the shortcomings of automated nucleic acid extraction equipment, the art is in urgent need of an improved device and/or equipment to improve the shortcomings of the prior art.

This summary is intended to provide a simplified summary of the disclosure to provide the reader with a basic understanding of the disclosure. This summary is not an extensive overview of the disclosure and it is not intended to identify key/critical elements of the embodiments of the invention or to delineate the scope of the invention.

In order to solve the problems existing in the prior art, the present invention provides a sample processing module that can complete the functions of up and down movement and rotation of the sleeve through a single driving component.

One aspect of the present invention relates to a sample processing module for use with a casing, wherein the sample processing module includes a first lifting plate, a fixed plate, a hollow solenoid and a first driving member. The first lifting plate includes a through hole and a first screw hole disposed on one side of the through hole. The fixed plate is provided with a second screw hole and is opposite to the first lifting plate. The hollow spiral tube includes a hollow body and a threaded portion provided on the outer surface of the hollow body. The hollow spiral tube has an upper end and a lower end. The upper end is coupled with the through hole of the first lifting plate, and the lower end is coupled with the through hole of the first lifting plate. The casing is detachably coupled, and the hollow spiral tube is threaded with the second screw hole of the fixing plate through the threaded portion, so that the hollow spiral tube penetrates the fixing plate. The first driving member includes a first driving rod and a first motor, wherein the first driving rod is screwed with the first screw hole of the first lifting plate, and the first motor is coupled with the first driving rod for driving. The first driving rod links the first lifting plate and the hollow solenoid to move up and down along the longitudinal axis, and rotates the hollow solenoid when moving.

According to some embodiments of the present disclosure, there are a plurality of through holes on the first lifting plate and they are arranged in an array.

According to a preferred embodiment of the present disclosure, there are a plurality of hollow coiled tubes arranged in an array.

According to another embodiment of the present disclosure, the sample processing module further includes a bearing, wherein the hollow spiral tube is coupled to the through hole through the bearing.

According to yet another embodiment of the present disclosure, the sample processing module further includes a second lifting plate, a magnetic rod and a second driving member. The second lifting plate includes a third screw hole. The magnetic rod has an upper end and a lower end, wherein the upper end is coupled to the second lifting plate, and the magnetic rod is configured to penetrate the hollow coil. The second driving member includes a second driving rod and a second motor, wherein the second driving rod is screwed with the third screw hole of the second lifting plate, and the second motor is coupled with the second driving rod for The second driving rod is driven to move the second lifting plate and the magnetic rod up and down along the longitudinal axis.

Another aspect of the present invention relates to a biological sample processing device for processing a biological sample, including a sample processing module of the present invention, a displacement module and a sample tank. The displacement module is coupled to the sample processing module of the present invention and used to move the sample processing module in the horizontal axis direction. The sample tank is used to contain biological samples, and the sample processing module is used to process the biological samples in the sample tank.

According to an embodiment of the present disclosure, the biological sample processing module further includes a sleeve detachably coupled with the hollow coil; when the hollow coil in the sample module moves up and down along the longitudinal axis When rotating, the casing is simultaneously connected to stir the biological sample. According to a preferred embodiment of the present disclosure, the sleeve is polygonal. More preferably, the sleeve further includes a plurality of ridges disposed on the outer surface of the sleeve.

After referring to the following embodiments, those with ordinary knowledge in the technical field to which the present invention belongs can easily understand the basic spirit and other objectives of the present invention, as well as the technical means and implementation modes adopted by the present invention.

The main component symbols of this new model are listed as follows:

100, 200, 400, 620: sample processing module

120, 220, 410: first lifting plate

122, 222, 422: Through hole

124:First screw hole

140, 240, 420: fixed plate

144:Second screw hole

160, 260, 430: hollow spiral tube

162: Hollow body

164: Upper end

166:lower end

168:Thread part

169:Bearing

180, 280, 440: first driving part

182:First drive rod

183:Thread

184:First motor

190, 290, 490, 680: Casing

192: convex strip

230, 450: Second lifting plate

232:Third screw hole

250, 460: Magnetic rod

252: Upper end

254:lower end

270, 470: Second driving part

272:Second drive rod

273:Thread

274:Second motor

600:Biological sample processing equipment

640:Displacement module

660:Sample tank

In order to make the above and other objects, features, advantages and embodiments of the present invention more obvious and understandable, the accompanying drawings are described as follows: Figure 1A shows a sample processing module according to certain embodiments of the present disclosure. Schematic diagram of 100; Figure 1B is a cross-sectional view along line 1B-1B of the sample processing module 100 in Figure 1A; Figure 2 is a schematic diagram of the sample processing module 200 according to another embodiment of the present disclosure; Figure 3A It is a schematic diagram of the second lifting plate, the magnetic rod and the second driving member according to the embodiment of Figure 2; Figure 3B is a cross-sectional view along line 3B-3B in Figure 3A; Figure 4 is based on this A schematic diagram of the sample processing module 400 shown in a specific embodiment of the disclosure; Figures 5A to 5D are schematic diagrams of the operation of the sample processing module 400 shown in an embodiment of the disclosure; and Figures 6A and 6 Figure 6B is a schematic configuration diagram of a biological sample processing device 600 according to different embodiments of the present disclosure.

In accordance with common practice, the various features and components in the figures are not drawn to scale but are drawn in a manner designed to best represent the specific features and components relevant to the present invention. In addition, the same or similar reference symbols are used to refer to similar elements/components in different drawings.

In order to make the description of the present disclosure more detailed and complete, the following provides an illustrative description of the implementation aspects and specific embodiments of the present invention; however, this is not the only form of implementing or using the specific embodiments of the present invention. The embodiments cover features of multiple specific embodiments as well as method steps and their sequences for constructing and operating these specific embodiments. However, other specific embodiments may also be used to achieve the same or equivalent functions and step sequences.

Unless otherwise defined in this specification, the scientific and technical terms used herein have the same meanings as commonly understood and customary by a person with ordinary knowledge in the technical field to which this invention belongs. Furthermore, on Discord In case of conflict below, the singular form of a noun used in this specification shall include the plural form of the noun; and the plural form of the noun shall also include the singular form of the noun.

The term "biological sample" refers to the biological sample suitable for processing by the biological sample processing equipment and/or sample processing module of the present invention. In one embodiment, the biological sample may be a biological sample used for nucleic acid extraction, such as culture fluid, blood, body fluid, animal tissue, plant tissue, virus, and eukaryotic and/or prokaryotic cells.

In this disclosure, the term "displacement module" refers to any mechanical module commonly known in the art for moving a component/module (for example, the sample processing module of the present invention) along the longitudinal and/or transverse directions. . In a non-limiting embodiment, the displacement module may be a robotic arm.

Unless otherwise defined in this specification, the scientific and technical terms used herein have the same meanings as commonly understood and customary by a person of ordinary skill in the art to which this invention belongs. In addition, unless there is conflict with the context, the singular noun used in this specification covers the plural form of the noun; and the plural noun used also covers the singular form of the noun.

II. Specific implementations

Currently, automated nucleic acid extraction equipment on the market mainly uses magnetic bead technology to extract nucleic acids. In order to achieve the purpose of extracting target nucleic acids from biological samples, it is crucial to mix biological samples with extraction reagents and magnetic beads to fully react. Therefore, existing Automated nucleic acid extraction equipment is usually equipped with two sets of driving components. One is used to grab and move samples or sleeves, and the other is used to drive the rotation of the sleeve to stir the mixture containing biological samples, extraction reagents and magnetic beads. Mixture. However, the number of driving parts not only increases the maintenance cost of the equipment, but also inevitably increases the volume of the overall equipment, requiring the user to provide more space to install the extraction equipment.

In order to solve the aforementioned shortcomings of automated nucleic acid extraction equipment, this disclosure provides a sample processing module. Through the design and linkage relationship of each component, a single driving component can be used to achieve the purpose of moving and driving the cannula rotation.

Figures 1A and 1B are schematic diagrams and cross-sectional views of the sample processing module 100 respectively. In order to clearly explain the configuration and arrangement of each component of the sample processing module 100, please refer to Figures 1A and 1B at the same time. Figure 1B. As shown in the figure, the sample processing module 100 includes a first lifting plate 120, a fixed plate 140, a hollow solenoid 160 and a first driving member 180, wherein the first lifting plate 120 is connected to the first driving member 180 respectively. The driving member 180 and the hollow solenoid 160 are coupled, and the hollow solenoid 160 is further coupled with the fixing plate 140 . With this arrangement, the first driving member 180 can be used to control the movement of the hollow solenoid 160 along the longitudinal axis, and the fixing plate 140 can be provided to cause the hollow solenoid 160 to rotate while moving. In one embodiment, in order to avoid sample contamination, the sample processing module 100 can be used with a sleeve 190 during operation, so that the sleeve 190 and the sample are in separate contact to avoid cross-contamination between two independent experiments. .

In the embodiment of the present disclosure, the configuration of the sleeve 190 can be a polygon or any other configuration that can generate a swirling flow in the liquid when rotating, so that different contents in the liquid can be mixed evenly. Preferably, a plurality of ridges 192 are provided on the outer surface of the sleeve 190 to increase the intensity of the swirling flow in the vertical direction and improve the mixing effect.

According to a preferred embodiment of the present disclosure, the sleeve 190 is a disposable consumable. Exemplary materials suitable for preparing the sleeve 190 include, but are not limited to, polymethyl methacrylate (PMMA). ; or acrylic), polyvinyl chloride (PVC), polytetrafluoroethylene (PTFE; or Teflon), thermoplastic polyurethane (TPU), polyether Etherketone (polyetheretherketone, PEEK), polyethylene (polyethylene, PE), polyether-polyamide block copolymer (polyether block amide, PEBA), etc.

According to the embodiment of the disclosure, the first lifting plate 120 includes a through hole 122 for coupling with the upper end 164 of the hollow solenoid 160 , and a first screw hole 124 for screwing with the first driving member 180 . It should be imagined that when the first driving member 180 drives the first lifting plate 120 to move up and down by screwing with the first screw hole 124, the hollow solenoid 160 and the first lifting plate 120 can be linked to rise or fall synchronously. . Available In optional embodiments, conventional methods may be used to couple the hollow coil 160 to the through hole 122 . In a preferred embodiment of the present disclosure, the hollow solenoid 160 is coupled with the through hole 122 using a bearing 169 so that the hollow solenoid 160 can rotate in the through hole 122 without falling off.

According to the embodiments of the present disclosure, a person with ordinary knowledge in the art can adjust the configuration of the first lifting plate 120 according to actual usage requirements to provide a plurality of through holes 122 (for example, 2, 3, 4, 5, 6, 7, 8 , 9, 10, 11, 12 or more), thereby operating a plurality of hollow solenoid tubes 160 at the same time. In one embodiment, the first lifting plate 120 is provided with 12 through holes 122 respectively coupled to the hollow coils 160 and arranged in an array (not shown in Figure 2). Depending on the purpose of use, more than one row of through holes 122 may be provided on the first lifting plate 120. For example, two, three, four, five, six, seven, eight or more rows may be provided (not shown in the figure). Show).

The fixing plate 140 is provided with a second screw hole 144 for screwing into the hollow solenoid tube 160 . In a preferred embodiment, the fixing plate 140 may be provided with a plurality of second screw holes 144 . For example, the fixing plate 140 may be configured according to the number of hollow solenoid tubes 160 coupled to the first lifting plate 120 . 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 or more second screw holes 144 are provided for screwing the hollow coiled tubes 160. In a preferred embodiment, the second screw holes 144 on the fixed plate 140 and the through holes 122 on the first lifting plate 120 have the same number and are arranged correspondingly up and down.

According to the embodiment of the present disclosure, during the operation of the sample processing module 100, the height position of the fixed plate 140 on the longitudinal axis will not change as the hollow solenoid 160 rotates and moves up and down. In a non-limiting embodiment, the fixing plate 140 is coupled to the first driving member 180 to fix its position (not shown in the figure).

The hollow solenoid 160 includes a hollow body 162 and a threaded portion 168 provided on its outer surface. Structurally, the hollow solenoid 160 has an upper end 164 and a lower end 166. The upper end 164 is rotatably connected to the through hole 122 of the first lifting plate 120, and the lower end 166 passes through the fixed plate 140 to be rotatably connected to the casing 190. Detachably coupled so that the sleeve 190 can be removed and replaced before the end and start of testing. As above, In a preferred embodiment of the present disclosure, the hollow solenoid 160 is coupled with the through hole 122 using a bearing 169 so that the hollow solenoid 160 can rotate in the through hole 122 without falling off. The threaded portion 168 is used for screwing with the second screw hole 144 of the fixing plate 140 .

In optional embodiments, those with ordinary knowledge in the art can select hollow solenoid tubes 160 with different pitches according to actual usage requirements to change the rotation speed (that is, the speed when stirring the sample). For example, if a longer pitch is used, The narrow hollow spiral tube 160 can increase its rotation speed; if the sample needs to be stirred slowly, a hollow spiral tube 160 with a wider pitch can be used.

The first driving member 180 includes a first driving rod 182 and a first motor 184. The first driving rod 182 is coupled with the first motor 184 and is driven to rotate by the first motor 184. According to the embodiment of the disclosure, the first motor 184 can be any electrical device that is well known for converting electrical energy into mechanical energy to drive other devices. For example, the first motor 184 can be a synchronous motor, an induction motor, Reversible motors, stepper motors, servo motors, linear motors or other kinds of motors.

In one embodiment, the outer surface of the first driving rod 182 is provided with threads 183 that are threadedly engaged with the first screw holes 124 of the first lifting plate 120 . Therefore, the first motor 184 drives the first driving rod 182 to rotate, and the threads in the first screw hole 124 will climb or descend along the threads on the outer surface of the first driving rod 182, thereby driving the first lifting plate 120 to move up and down. It also drives the hollow solenoid 160 to move up and down along the longitudinal axis. At the same time, through the second screw hole 144 on the fixed plate 140, the hollow solenoid 160 rotates clockwise or counterclockwise while moving, and drives the casing 190 to synchronize. Rotate to stir the sample. Such a configuration allows the sample processing module 100 of the present invention to only need one set of driving parts (ie, the first driving part 180) to drive the sleeve 190 to move and rotate, which is one of the advantages of the present invention.

Please refer to Figure 2. According to some other embodiments of the present disclosure, the sample processing module 200 of the present invention, in addition to the components of the sample processing module 100 described in Figure 1, may further include a second lifting plate 230 and a magnetic rod. 250 and the second driving member 270 are used in the experimental procedures of the magnetic bead technology platform. The components of the sample processing module 100 shown in Figure 1 will not be described again here. In principle, the second lifting plate 230 and the magnetic The structures and operating modes of the rod 250 and the second driving member 270 are similar to the first lifting plate 220 , the first driving member 280 and the hollow solenoid 260 .

Specifically, please refer to Figure 3A and Figure 3B at the same time, wherein Figure 3A is a schematic assembly diagram of the second lifting plate 230, the magnetic rod 250 and the second driving member 270 according to these embodiments; Figure 3B The figure is a cross-sectional view of Figure 3A to further illustrate the arrangement relationship of each component. The second driving member 270 includes a second driving rod 272 and a second motor 274. The second driving rod 272 is coupled with the second motor 274 and has threads 273 on its outer surface.

A third screw hole 232 is provided on one side of the second lifting plate 230 for screw connection with the second driving rod 272 of the second driving member 270 , and is provided on the other side for coupling with the magnetic rod 250 . The magnetic rod 250 has an upper end 252 and a lower end 254. The upper end 252 is connected to the bottom of the second lifting plate 230, and the lower end 254 is used to penetrate the hollow solenoid 260 and the casing 290. In terms of configuration, according to a preferred embodiment, the diameter of the magnetic rod 250 is smaller than the diameter of the through hole 222, the hollow solenoid tube 260 and the sleeve 290, so that the magnetic rod 250 can be inserted into the hollow spiral tube through the through hole 222. Inside the tube 260 and the sleeve 290 (please refer to Figure 2). During actual operation, the second driving member 270 can make the second lifting plate 230 move up and down along the second driving rod 272 . According to the embodiment of the present disclosure, the second motor 274 can be any electrical device that is well known for converting electrical energy into mechanical energy to drive other devices. For example, the second motor 274 can be a synchronous motor, an induction motor, Reversible motors, stepper motors, servo motors, linear motors or other kinds of motors.

During actual operation, the first driving member 280 drives the first lifting plate 220 and the hollow solenoid 260 to move up and down along the longitudinal direction. The bottom of the hollow solenoid 260 can be detachably connected to the sleeve 290 . The second driving member 270 drives the second lifting plate 230 to rise and fall, and the linked magnetic rod 250 moves up and down along the longitudinal axis direction, and is inserted into the hollow solenoid 260 and the sleeve 290. The sample processing module 200 proposed by the present invention uses the above-mentioned The configuration can complete the sample processing steps, and those with ordinary knowledge in the technical field will understand that the sample processing module of the present invention can be used to process a large number of biological samples to improve inspection efficiency.

FIG. 4 illustrates a sample processing module 400 according to a specific embodiment of the present disclosure, which can process multiple samples simultaneously. Therefore, in this embodiment, the sample processing module 400 is provided with a plurality of hollow solenoid tubes 430 and a plurality of magnetic rods 460 . Structurally, the first lifting plate 410 is provided with a plurality of through holes 422 that are respectively coupled to the upper ends of the hollow coils 430, and the lower ends of the hollow coils 430 can be detachably connected to the casing 490 as needed. The connection method is the same as shown in Figure 1 and will not be described again. The upper ends of the plurality of magnetic rods 460 are connected to the bottom of the second lifting plate 450 . In a preferred embodiment, the number of magnetic rods 460 and hollow solenoid tubes 430 is the same. As shown in the figure, in this embodiment, the first lifting plate 410 is connected to 12 hollow solenoid tubes 430, wherein the hollow solenoid tubes 430 are arranged in an array, and a corresponding number of 12 magnetic rods 460 are provided on the second lifting plate 450 and are also arranged in an array, and each magnetic rod 460 is configured to pass through the hollow solenoid 430 and the casing 490 respectively. , it is driven to move up and down along the longitudinal axis.

Next, the operation mode of the sample processing module 400 of the present invention is further explained through Figures 5A to 5D.

In one embodiment, the sample processing module 400 of the present invention is suitable for the technology of using magnetic beads to extract nucleic acids in samples. For convenience of explanation, this figure is illustrated with a side view to simplify the operation mode of the sample processing module 400. . Specifically, in the nucleic acid extraction step, a plurality of nucleic acid-containing samples to be extracted are added to the sample tank (or any container that can be used to hold these reactants), and the extraction reagent (for example, lysis) is added as necessary according to the steps. buffer, binding buffer, wash buffer or elution buffer) and/or magnetic bead mixing. For example, before processing the sample, the step of taking the sleeve 490 is first performed to activate the first driving member 440, so that the first lifting plate 410 begins to descend and is detachably connected to the upper end of the sleeve 490. Next, in the step of sample processing, the first driving member 440 is started and the sleeve 490 is driven down to contact the sample in the sample tank. At the same time, through the configuration of the hollow solenoid 430 and the fixed plate 420, the hollow solenoid 430 is driven to rotate. And drive the casing 490 to rotate synchronously (Fig. 5A), and then drive the first lifting plate 410 to rise through the first driving member 440, so that the casing 490 rises and rotates in the opposite direction (Fig. 5B). Repeat the above action to perform the sample Mixing step in the tank (i.e. repeat the steps in Figure 5A and Figure 5B). Furthermore, depending on the needs of the magnetic bead extraction method, start The second driving member 470 is moved to lower the magnetic rod 460 and penetrate into the hollow solenoid 430 and the casing 490 (Fig. 5C). The magnetic force of the magnetic rod 460 attracts the magnetic beads and makes them adhere to the outside of the casing 490. , preferably gathered at the bottom of the sleeve 490. If you want to suspend the magnetic beads in the sample again, start the second driving member 470 to make the magnetic rod 460 rise and move away from the sleeve 490. The magnetic beads will be suspended again in the sample. in the sample. If the magnetic beads need to be removed from the sample, the first driving member 440 and the second driving member 470 can be activated simultaneously to move the sleeve 490 and the magnetic rod 460 out of the sample, and then the magnetic beads can be removed.

Figures 6A and 6B are schematic configuration diagrams of biological sample processing equipment 600 according to different embodiments of the present disclosure. In a preferred embodiment, the biological sample processing equipment 600 is an automated nucleic acid (eg, RNA or DNA) extraction system capable of simultaneously processing multiple biological samples. Specifically, as shown in Figure 6A, the biological sample processing device 600 includes the sample processing module 620 according to any embodiment of the present disclosure, and a displacement module 640 coupled to the sample processing module 620. According to Move the sample processing module 620 laterally. In addition, the biological sample processing equipment also includes a sample tank 660, where the sample tank 660 is used to contain different biological samples and/or extraction reagents required for different steps.

The overall operation mode of the biological sample processing device 600 is substantially the same as that described in Figures 5A to 5D, except that the biological sample processing device includes a displacement module 640, which can carry the sample processing module in a sample tank containing different reaction reagents. Move between spaces and extend into the holes for magnetic attraction and/or mixing to automatically complete the entire extraction process and obtain nucleic acid products.

Figure 6B shows a biological sample processing device 600 according to another embodiment of the present invention. In this embodiment, the biological sample processing device 600 further includes a casing cassette containing a plurality of casings 680 for the sample processing module 620 The arrangement of the casing 680 and the sample processing module 620 is as described in Figures 1 to 4, and therefore will not be described again here.

To summarize the above, the present disclosure provides a sample processing module and a biological sample processing device including the sample processing module, which has the advantage of being able to complete longitudinal movement through a single drive module. And the action of rotating and stirring achieves the purpose of reducing the maintenance cost of automated nucleic acid extraction equipment and reducing the size of the equipment.

Although the above embodiments disclose specific examples of the present invention, they are not intended to limit the present invention. Those with ordinary knowledge in the technical field to which the present invention belongs can, without departing from the principles and spirit of the present invention, Various changes and modifications can be made to it, so the protection scope of the present invention shall be defined by the appended patent application scope.

400:Sample processing module

410:First lifting plate

420:fixed plate

430:Hollow spiral tube

440: first drive part

450:Second lifting plate

460:Magnet rod

470: Second drive part

490:casing

Claims (10)

A sample processing module for use with a set of tubes, including A first lifting plate, including: through holes; and A first screw hole is provided on one side of the through hole; A fixed plate is provided with a second screw hole, and the fixed plate is arranged opposite to the first lifting plate; A hollow solenoid tube, including a hollow body and a threaded portion provided on the outer surface of the hollow body, wherein the hollow solenoid tube has an upper end and a lower end, and the upper end is coupled with the through hole, and the hollow solenoid tube is passed through the through hole. The threaded portion is threaded with the second screw hole of the fixing plate, and the hollow solenoid is penetrated on the fixing plate, and the lower end of the hollow solenoid is used to detachably couple with the casing; and A first driver component, including: A first driving rod screwed with the first screw hole of the first lifting plate; and A first motor coupled to the first driving rod for driving the first driving rod to link the first lifting plate and the hollow solenoid to move up and down along the longitudinal axis, and the hollow solenoid rotates when moving . The sample processing module as described in request 1 further includes: a second lifting plate, including a third screw hole on the second lifting plate; A magnetic rod having an upper end and a lower end, wherein the upper end is coupled to the second lifting plate, and the magnetic rod is configured to penetrate the hollow solenoid; and a second driver component, including: a second driving rod screwed with the third screw hole of the second lifting plate; and A second motor is coupled to the second driving rod and used to drive the second driving rod to link the second lifting plate and the magnetic rod to move up and down along the longitudinal axis direction. The sample processing module of claim 1 further includes a bearing, wherein the hollow spiral tube is coupled to the through hole through the bearing. The sample processing module as claimed in claim 1, wherein the lower end of the hollow spiral tube is used to engage with the sleeve. The sample processing module as claimed in claim 1, wherein the through holes on the first lifting plate are plural and arranged in an array. The sample processing module as described in claim 1, wherein the hollow spiral tubes are plural and arranged in an array. A biological sample processing equipment used to process a biological sample, including: A sample processing module as described in claim 1; A moving module is coupled to the sample processing module and used to move the sample processing module in the horizontal axis direction; and A sample tank is used to hold the biological sample, and the sample processing module is used to process the biological sample in the sample tank. The biological sample processing equipment according to claim 7, further comprising a sleeve for detachably coupling with the hollow solenoid, wherein when the hollow solenoid in the sample processing module is driven along the longitudinal axis While moving up and down and rotating, the biological sample is stirred at the same time. The biological sample processing equipment of claim 8, wherein the sleeve is polygonal. The biological sample processing equipment as claimed in claim 9, wherein the sleeve further includes a plurality of ridges disposed on the outer surface of the sleeve.