CN218290915U

CN218290915U - Full-automatic nucleic acid extraction appearance

Info

Publication number: CN218290915U
Application number: CN202222233954.XU
Authority: CN
Inventors: 吴立群; 齐建国; 李小明; 马骋; 吴思坪; 陈莉
Original assignee: Bomaide Biotechnology Gu'an Co ltd
Current assignee: Bomaide Biotechnology Gu'an Co ltd
Priority date: 2022-08-24
Filing date: 2022-08-24
Publication date: 2023-01-13
Anticipated expiration: 2032-08-24

Abstract

The utility model discloses a full-automatic nucleic acid extraction appearance, include: experiment cabin, the bottom fixedly connected with installing frame of experiment cabin inner wall, the bottom of experiment cabin inner wall is located the first motor of the inside fixedly connected with of installing frame, the output fixedly connected with of first motor adjusts the lead screw, the back sliding connection of experiment cabin inner wall has the bearing seat, the top of adjusting the lead screw runs through installing frame and bearing seat in proper order and extends to the top of experiment cabin inner wall, the positive top fixedly connected with mounting panel of bearing seat, the bottom of mounting panel has a plurality of bar magnet framves from a left side to the right side evenly running through. The utility model discloses a start first motor and drive the regulation lead screw and rotate, can drive mounting panel and four bar magnet framves through the bearing seat then and reciprocate simultaneously, can take a sample to four reagent stirring frame insides simultaneously, degree of automation is high, and the flux is big, can draw thirty-two samples simultaneously at every turn, has effectively improved nucleic acid and has drawed detection efficiency.

Description

Full-automatic nucleic acid extraction appearance

Technical Field

The utility model relates to the technical field of medical equipment, specifically a full-automatic nucleic acid extraction appearance.

Background

Nucleic acid is a biological macromolecular compound polymerized by a plurality of nucleotides, is one of the most basic substances of life, and is widely existed in all animal and plant cells and microorganisms, nucleic acid in organisms is often combined with protein to form nucleoprotein, in recent years, with the rapid development of molecular biology, molecular diagnosis and detection technology based on nucleic acid increasingly shows a crucial role in various fields, nucleic acid required for experiment and detection needs to be purified and extracted from biological samples, but purification of a plurality of samples is quite difficult, the process not only needs to select proper purification technology, but also has extremely large workload, and is difficult to meet the requirement of extraction and purification of high-throughput samples which is rapidly developed at present.

The existing nucleic acid extraction instruments all adopt a magnetic bead method to extract nucleic acid, the nucleic acid extraction instrument used in the extraction process is an instrument which automatically finishes the extraction work of sample nucleic acid by using a matched magnetic bead nucleic acid extraction reagent, the nucleic acid extraction demand is increased due to the requirement of disease control, the flux of the existing nucleic acid extraction instrument is small, the number of extraction stations of the nucleic acid extraction instrument is small, and the nucleic acid extraction efficiency is reduced.

Therefore, it is necessary to provide a fully automatic nucleic acid extractor to solve the above problems.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a full-automatic nucleic acid extraction appearance to solve the little problem of nucleic acid extraction flux of proposing among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: a full-automatic nucleic acid extractor, comprising: experiment cabin, the bottom fixedly connected with installing frame of experiment cabin inner wall, the bottom of experiment cabin inner wall is located the first motor of inside fixedly connected with of installing frame, the output fixedly connected with of first motor adjusts the lead screw, the back sliding connection of experiment cabin inner wall has the bearing seat, the top of adjusting the lead screw runs through installing frame and bearing seat in proper order and extends to the top of experiment cabin inner wall, the positive top fixedly connected with mounting panel of bearing seat, the bottom of mounting panel has evenly run through a plurality of bar magnet framves from a left side to the right side, the top of installing frame is located every bar magnet frame from a left side to the right side and has all run through the reagent stirring frame under, the bar magnet frame includes the fixing base, the fixing base is fixed in the bottom of mounting panel that runs through, the fixed conductive seat that runs through on the fixing base, the top fixedly connected with spring wire of conductive seat, the bottom fixed connection bar of conductive seat, the bottom magnetism of conductive seat is inhaled the stick and is equipped with the stirring cover, the bottom of stirring cover is inhaled through magnetism and is fixed with the magnetic bead.

Preferably, reagent stirring frame includes eight places axle, second motor and stop collar, and eight places the axle from the front to the top of back evenly run through in the installing frame, stop collar fixed connection locates eight outside of placing the axle in the top of installing frame and cover, every place the axial plane of axle and lie in the inside of installing frame and all fix the cover and be equipped with drive gear, two adjacent drive gear intermeshing, second motor fixed connection is in the bottom of experiment cabin inner wall and lie in one and place the bottom of axle.

Preferably, the output of second motor and one bottom fixed connection of placing the axle, eight logical grooves have evenly been seted up to the inside in the past to the back of stop collar, the top of placing the axle is located logical inslot portion and has seted up the recess.

Preferably, the adjusting screw rod is in threaded connection with the bearing seat, the adjusting screw rod is rotatably connected with the mounting frame, and the adjusting screw rod is rotatably connected with the experiment cabin.

Preferably, the front face of the experiment chamber is hinged with a chamber door through a hinge.

Preferably, a touch control panel is arranged at the top of the front face of the experiment chamber.

Preferably, the placing shaft is rotatably connected with the mounting frame.

Compared with the prior art, the beneficial effects of the utility model are that: according to the full-automatic nucleic acid extraction instrument, the first motor is started to drive the adjusting screw rod to rotate, then the mounting plate and the four magnetic rod racks can be driven by the bearing seat to move up and down simultaneously, the interiors of the four reagent stirring racks can be sampled simultaneously, the automation degree is high, the flux is large, thirty-two samples can be extracted simultaneously each time, and the nucleic acid extraction detection efficiency is effectively improved; can drive eight simultaneously through a second motor and place the axle and rotate simultaneously, same power is connected to the inside second motor of four reagent stirring framves, can drive eight respectively and place the axle and rotate simultaneously, the inside stirring cover of cooperation bar magnet frame stirs test tube inside, rapid mixing liquid repeatedly, make the even mixture of liquid and magnetic bead, finally obtain high-purity nucleic acid, the extraction efficiency of DNA and RNA in samples such as effectively improved animal and plant tissue, blood, the body fluid, criminal specimen.

Drawings

FIG. 1 is a front view of the present invention;

FIG. 2 is a main sectional view showing the structure of the present invention;

FIG. 3 is a schematic sectional view of the present invention;

FIG. 4 is a schematic front cross-sectional view of the structure of the magnet bar holder of FIG. 2;

FIG. 5 is a schematic top view of the fixing base shown in FIG. 4;

FIG. 6 is a schematic main sectional view of the reagent stirring rack shown in FIG. 2;

FIG. 7 is a schematic top view of the stop collar of FIG. 6;

fig. 8 is a bottom view schematically showing the structure of the transmission gear shown in fig. 6.

In the figure: 1. an experiment cabin; 2. installing a frame; 3. a first motor; 4. adjusting the lead screw; 5. a load bearing seat; 6. mounting a plate; 7. a magnetic rod rack; 8. a reagent stirring rack; 9. a cabin door; 71. a fixed seat; 72. a conductive seat; 73. a spring wire; 74. a magnetic suction rod; 75. a stirring sleeve; 76. magnetic beads; 81. placing a shaft; 82. a second motor; 83. a transmission gear; 84. a limiting sleeve.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-8, the present invention provides a technical solution: a full-automatic nucleic acid extractor, comprising: the experimental chamber 1, the bottom of the inner wall of the experimental chamber 1 is fixedly connected with an installation frame 2, the bottom of the inner wall of the experimental chamber 1 is positioned inside the installation frame 2 and is fixedly connected with a first motor 3, the output end of the first motor 3 is fixedly connected with an adjusting screw rod 4, the back of the inner wall of the experimental chamber 1 is slidably connected with a bearing seat 5, the top end of the adjusting screw rod 4 sequentially penetrates through the installation frame 2 and the bearing seat 5 and extends to the top of the inner wall of the experimental chamber 1, the top of the front surface of the bearing seat 5 is fixedly connected with a mounting plate 6, the bottom of the mounting plate 6 is uniformly penetrated with a plurality of magnetic rod frames 7 from left to right, the top of the installation frame 2 is positioned right under each magnetic rod frame 7 from left to right and is penetrated with a reagent stirring frame 8, each magnetic rod frame 7 comprises a fixed seat 71, the fixed seat 71 is fixedly penetrated at the bottom of the mounting plate 6, a conductive seat 72 is fixedly penetrated on the fixed seat 71, the top end of the conductive seat 72 is fixedly connected with a spring wire 73, the bottom end of the conductive seat 72 is fixedly connected with a magnetic rod 74, the bottom end of the magnetic rod 74 is sleeved with a stirring sleeve 75, the magnetic absorption control panel on the front surface of the magnetic absorption control chamber 1, an external power supply is transmitted to the conductive seat 72 through the spring wire 73 and then transmitted to the magnetic attraction rod 74 through the conductive seat 72, the magnetic attraction rod 74 has magnetic force after being electrified, the magnetic attraction rod 74 magnetically attracts and sleeves the stirring sleeve 75 made of metal through the magnetic force, so that the stirring sleeve 75 also has magnetic force to adsorb the magnetic beads 76, the first motor 3 is connected with an external power supply and is a three-phase asynchronous motor, and the power supply is controlled by a touch control panel at the top of the front surface of the experiment chamber 1, the number of the conductive seats 72 is eight, and the conductive seats uniformly penetrate through the fixed seat 71 from front to back.

Referring to fig. 2-8, reagent stirring frame 8 includes eight shafts 81 of placing, second motor 82 and stop collar 84, eight shafts 81 of placing evenly run through in the top of installing frame 2 after to in the past, stop collar 84 fixed connection locates eight outside of placing shafts 81 at the top of installing frame 2 and cover, every shaft face of placing shafts 81 is located the inside of installing frame 2 and all fixes the cover and is equipped with drive gear 83, two adjacent drive gear 83 intermeshing, second motor 82 fixed connection is in the bottom of experiment cabin 1 inner wall and is located one and places the bottom of shaft 81, external power source is all connected to the inside second motor 82 of four reagent stirring frames 8, and control the panel through the touch-control and be provided with same switch.

Referring to fig. 2-8, the output end of the second motor 82 is fixedly connected with the bottom end of a placing shaft 81, eight through grooves are uniformly formed in the limiting sleeve 84 from front to back, the top end of the placing shaft 81 is located in each through groove and is provided with a groove, and eight grooves in the top end of the placing shaft 81 in each reagent stirring frame 8 can be used for simultaneously placing eight nucleic acid test tubes.

Referring to fig. 2-3, the adjusting screw rod 4 is in threaded connection with the bearing seat 5, the adjusting screw rod 4 is in rotational connection with the mounting frame 2, the adjusting screw rod 4 is in rotational connection with the experiment chamber 1, the bearing seat 5 is limited by the sliding connection with the back of the inner wall of the experiment chamber 1, and when the adjusting screw rod 4 rotates forwards and backwards, the bearing seat 5 can be driven to move up and down.

Referring to fig. 1, a hatch door 9 is hinged to the front of the experiment chamber 1 through a hinge, a glass plate is fixedly penetrated through the front of the hatch door 9, so that the condition of nucleic acid detection in the experiment chamber 1 can be observed through the glass plate, and a handle is fixedly embedded in the top of the front of the hatch door 9, so that the hatch door 9 can be opened or closed conveniently.

Referring to fig. 1, a touch control panel is disposed on the top of the front side of the experiment chamber 1, and the touch control panel is connected to an external power source and can control the experiment chamber 1 to perform a nucleic acid experiment operation.

Referring to fig. 2-8, the placing shaft 81 is rotatably connected to the mounting frame 2, and the eight conductive seats 72 inside each magnetic rod rack 7 are aligned with the top ends of the eight placing shafts 81 inside each reagent stirring rack 8.

The working principle is as follows: as shown in FIGS. 1-8, when using the full-automatic nucleic acid extractor, first, a plurality of nucleic acid reagent tubes are respectively placed in eight through grooves on a position-limiting sleeve 84 inside four reagent stirring frames 8, a groove at the top end of a placing shaft 81 is used for placing the nucleic acid reagent tubes, then four second motors 82 are started, each second motor 82 can drive one placing shaft 81 to rotate, so as to drive one transmission gear 83 to rotate, adjacent transmission gears 83 are meshed, one transmission gear 83 can sequentially drive other seven transmission gears 83 to rotate simultaneously, so as to drive eight transmission gears 83 inside each reagent stirring frame 8 to rotate simultaneously, so as to drive eight placing shafts 81 to rotate simultaneously, so as to drive the nucleic acid reagent tubes placed at the top of the placing shaft 81 to rotate simultaneously, then the first motor 3 is started, an output shaft of the first motor 3 drives an adjusting screw 4 to rotate, the bearing seat 5 is connected to the back of the inner wall of the experiment chamber 1 in a sliding manner and is limited, when the screw rod 4 is adjusted to rotate forward, the bearing seat 5 is driven to move downwards, then four magnetic rod racks 7 can be driven to move downwards simultaneously, the stirring sleeves 75 below eight conductive seats 72 inside the magnetic rod racks 7 move downwards to the inside of the nucleic acid reagent tube, the spring wire 73 is closed to connect a power supply, the magnetic force of the stirring sleeves 75 is disconnected, magnetic beads 76 fall into the stirring sleeves 75, along with the rotation of the nucleic acid reagent tube, the magnetic beads 76 are fully stirred inside the nucleic acid reagent tube by the stirring sleeves 75, high-purity nucleic acid is finally obtained through cell cracking, nucleic acid adsorption, cleaning and elution, nucleic acid reagent is uniformly adhered to the surface of each magnetic bead 76, the power supply connected with the spring wire 73 is started, the magnetic rod 74 is electrified through the conductive seats 72, the magnetic beads 76 in the stirring sleeves 75 and the nucleic acid reagent are adsorbed, then, the reverse rotation switch of the output end of the first motor 3 is started, the adjusting screw rod 4 is driven to rotate reversely, then, the bearing seat 5 can be driven to move upwards, the mounting plate 6 is driven to move upwards, the four magnetic rod racks 7 adhered with nucleic acid reagents are integrally moved upwards, each reagent stirring rack 8 can prevent eight nucleic acid reagent tubes, thirty-six nucleic acid reagent tubes are taken out from the inside of the experiment chamber 1, the thirty-six reaction reagent tubes are respectively placed inside the eight through grooves of the limiting sleeves 84 inside the four reagent stirring racks 8 and are placed in the grooves at the top ends of the placing shafts 81, then, the forward rotation switch of the first motor 3 is started, then, the four magnetic rod racks 7 adhered with nucleic acid reagents can be driven to move downwards simultaneously, then, the magnetic beads 76 adsorbed with nucleic acids can be moved to the inside of the reaction test tubes, nucleic acid detection is carried out, the detection efficiency of the nucleic acid reagents is effectively improved, and the characteristic of the full-automatic nucleic acid extractor is achieved.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A full-automatic nucleic acid extractor, comprising: experiment cabin (1), its characterized in that, the bottom fixedly connected with installing frame (2) of experiment cabin (1) inner wall, the bottom of experiment cabin (1) inner wall is located the first motor (3) of inside fixedly connected with of installing frame (2), the output fixedly connected with of first motor (3) adjusts lead screw (4), the back sliding connection of experiment cabin (1) inner wall has bearing seat (5), the top of adjusting lead screw (4) runs through installing frame (2) and bearing seat (5) in proper order and extends to the top of experiment cabin (1) inner wall, the positive top fixedly connected with mounting panel (6) of bearing seat (5), the bottom of mounting panel (6) evenly runs through from a left side to the right side has a plurality of bar magnet racks (7), the top of installing frame (2) is located all to run through under every bar magnet rack (7) has reagent stirring frame (8), bar rack (7) includes from a left side to right side, fixing base (71) is fixed to run through in the bottom of mounting panel (6), the fixed seat (71) is gone up the fixed wire that runs through the conducting rod (72), magnetism is inhaled the bottom is equipped with the conducting rod (72) and is connected with the bottom (72) of conducting rod (72), and magnetic beads (76) are fixed at the bottom end of the stirring sleeve (75) through magnetic attraction.

2. The automatic nucleic acid extractor of claim 1, wherein: reagent stirring frame (8) are including eight axle (81), second motor (82) and stop collar (84) of placing, and eight are placed axle (81) and are evenly run through in the top of installing frame (2) after to in the past, stop collar (84) fixed connection locates eight outside of placing axle (81) at the top of installing frame (2) and cover, every the axial plane of placing axle (81) is located the inside of installing frame (2) and all fixes the cover and be equipped with drive gear (83), two adjacent drive gear (83) intermeshing, second motor (82) fixed connection is in the bottom of experiment cabin (1) inner wall and is located one and places the bottom of axle (81).

3. The automatic nucleic acid extractor of claim 2, wherein: the output of second motor (82) and one bottom fixed connection of placing axle (81), eight logical grooves have evenly been seted up to the inside in the past to the back of stop collar (84), the top of placing axle (81) is located logical inslot portion and has seted up the recess.

4. The automatic nucleic acid extractor of claim 1, wherein: the adjusting screw rod (4) is in threaded connection with the bearing seat (5), the adjusting screw rod (4) is rotatably connected with the mounting frame (2), and the adjusting screw rod (4) is rotatably connected with the experiment cabin (1).

5. The automatic nucleic acid extractor of claim 1, wherein: the front of the experiment cabin (1) is hinged with a cabin door (9) through a hinge.

6. The automatic nucleic acid extractor of claim 1, wherein: and a touch control type control panel is arranged at the top of the front side of the experiment cabin (1).

7. The automatic nucleic acid extractor of claim 2, wherein: the placing shaft (81) is rotatably connected with the mounting frame (2).