CN111849745A - Nucleic acid extractor capable of greatly improving nucleic acid extraction concentration - Google Patents
Nucleic acid extractor capable of greatly improving nucleic acid extraction concentration Download PDFInfo
- Publication number
- CN111849745A CN111849745A CN202010789547.XA CN202010789547A CN111849745A CN 111849745 A CN111849745 A CN 111849745A CN 202010789547 A CN202010789547 A CN 202010789547A CN 111849745 A CN111849745 A CN 111849745A
- Authority
- CN
- China
- Prior art keywords
- nucleic acid
- instrument body
- acid extraction
- fixedly connected
- purification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 64
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 64
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 64
- 238000000605 extraction Methods 0.000 title claims abstract description 47
- 238000000746 purification Methods 0.000 claims abstract description 30
- 238000012360 testing method Methods 0.000 claims abstract description 13
- 238000012545 processing Methods 0.000 claims description 19
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 9
- 230000005540 biological transmission Effects 0.000 claims description 9
- 238000001179 sorption measurement Methods 0.000 claims description 7
- 230000002070 germicidal effect Effects 0.000 claims description 6
- 239000011941 photocatalyst Substances 0.000 claims description 6
- 239000002131 composite material Substances 0.000 claims description 4
- 230000008878 coupling Effects 0.000 claims description 2
- 238000010168 coupling process Methods 0.000 claims description 2
- 238000005859 coupling reaction Methods 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 abstract description 10
- 238000001914 filtration Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 14
- 239000011324 bead Substances 0.000 description 10
- 239000007788 liquid Substances 0.000 description 9
- 239000000243 solution Substances 0.000 description 7
- 230000001954 sterilising effect Effects 0.000 description 7
- 150000002500 ions Chemical class 0.000 description 5
- 230000009471 action Effects 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 2
- 238000005336 cracking Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000003472 neutralizing effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000012149 elution buffer Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000011534 wash buffer Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultraviolet radiation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/16—Disinfection, sterilisation or deodorisation of air using physical phenomena
- A61L9/18—Radiation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/16—Disinfection, sterilisation or deodorisation of air using physical phenomena
- A61L9/22—Ionisation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Biomedical Technology (AREA)
- Animal Behavior & Ethology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a nucleic acid extraction instrument capable of greatly improving the concentration of extracted nucleic acid, which comprises a bottom frame and an instrument body fixedly connected to the top of the bottom frame, wherein a test tube rack is connected to the bottom of the inner wall of the instrument body in a sliding manner, and a purification assembly is arranged on the back of the top of the instrument body. This improve nucleic acid extraction appearance of nucleic acid extraction concentration by a wide margin, this test-tube rack can push the inside use that comes into operation of instrument body automatically through sterilamp rear, personnel can take the operation to the test-tube rack in the instrument outside, avoid operator and the inside contact of extraction appearance, simultaneously through inside air suction purifying box of air exhauster with the inside of instrument body, can carry to the instrument body inside via inside purification subassembly filtration purification rear, avoid instrument body inside air to breed the bacterium, both cooperations of propelling movement subassembly and purification subassembly can improve quality and the concentration that nucleic acid extracted to a certain extent.
Description
Technical Field
The invention relates to the technical field of nucleic acid extraction, in particular to a nucleic acid extraction instrument for greatly improving the concentration of nucleic acid extraction.
Background
The nucleic acid extractor is an instrument for automatically completing the extraction of sample nucleic acid by using a matched nucleic acid extraction reagent, and is widely applied to various fields of disease control centers, clinical disease diagnosis, blood transfusion safety, forensic medicine identification, environmental microorganism detection, food safety detection, animal husbandry, molecular biology research and the like, and the magnetic bead method nucleic acid extractor is generally divided into a pumping method and a magnetic rod method: 1. the suction method, also called a pipetting method, realizes the extraction of nucleic acid by fixing magnetic beads and transferring liquid, and generally realizes the transfer by controlling a mechanical arm by an operating system, wherein the extraction process comprises the following steps of 1) cracking: adding lysis solution into a sample, uniformly mixing and fully reacting the reaction solution through mechanical movement and heating, cracking cells, releasing nucleic acid, and 2) adsorbing: add the magnetic bead in the sample lysate, the abundant mixing utilizes the magnetic bead to have the characteristics of very strong affinity to nucleic acid under the low pH value of high salt, adsorbs nucleic acid, and under the effect of external magnetic field, magnetic bead and solution separation utilize the suction head to shift out liquid and abandon to the waste liquid groove, and the suction head is abandoned, 3) the washing: removing the external magnetic field, adding a new suction head into the washing buffer solution, fully and uniformly mixing, removing impurities, moving out the liquid under the action of the external magnetic field, and 4) eluting: removing the external magnetic field, adding a new suction head into an elution buffer solution, fully and uniformly mixing, separating the combined nucleic acid from the magnetic beads to obtain purified nucleic acid, and 2. the magnetic rod method realizes the separation of the nucleic acid by fixing liquid and transferring the magnetic beads, has the same principle and process as the suction method, has the difference of a magnetic bead and liquid separation mode, and separates the magnetic beads from waste liquid by the adsorption of the magnetic rods on the magnetic beads, and puts the waste liquid into the next liquid to realize the extraction of the nucleic acid.
When the nucleic acid extractor is used, a person is required to take and place a sample frame, the contact between an operator and the inside of the extractor is increased by repeated operation, the possibility that the person pollutes a sample exists, and meanwhile, because the inside is in a closed state, the inside gas is not circulated and the outside air is directly introduced into the extractor, bacteria are easy to breed, so that the inside of the extractor is polluted, the extraction efficiency and the extraction quality of nucleic acid are reduced, and therefore, the invention improves the extraction concentration of the nucleic acid in order to greatly improve the extraction quality of the nucleic acid and increase the extraction concentration of the nucleic acid.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides the nucleic acid extractor which can greatly improve the concentration of extracted nucleic acid, and solves the problems that personnel take and place a sample frame, contact between an operator and the interior of the extractor is increased due to repeated operation, the possibility of sample pollution of the personnel exists, and meanwhile, the interior of the extractor is easy to breed bacteria due to non-circulation of gas, so that the interior of the extractor is polluted, and the concentration of extracted nucleic acid is reduced.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: a nucleic acid extraction instrument capable of greatly improving nucleic acid extraction concentration comprises a bottom frame and an instrument body fixedly connected to the top of the bottom frame, wherein a test tube rack is connected to the bottom of the inner wall of the instrument body in a sliding mode, a purification assembly is arranged on the back of the top of the instrument body, and a pushing assembly is arranged inside the bottom frame;
the propelling movement subassembly includes the dwang, the front and the back rotation that the both ends of dwang all passed through bearing and underframe inner wall are connected, connecting block fixedly connected with motor is passed through at the back of underframe bottom, and the first belt pulley of shaft coupling fixedly connected with is passed through to the one end of motor output shaft, one side fixedly connected with second belt pulley on dwang surface, the fixed surface of dwang is connected with the rotating sleeve, and rotates telescopic surface and has seted up the arc driving groove, and the inner wall sliding connection in arc driving groove has the slide bar, the inside that underframe and instrument body and extend to the instrument body is run through in proper order on the top of slide bar, the slide bar extends to the bottom fixed connection of the inside one end of instrument body and test-tube rack.
Preferably, the surface of second belt pulley is connected with the surface transmission of first belt pulley through the belt, the purification subassembly includes the purifying box, the bottom of purifying box and the top fixed connection of instrument body.
Preferably, the top of purifying box inner wall passes through connecting block fixedly connected with air exhauster, and the air intake intercommunication of air exhauster has the air-supply line.
Preferably, one end of the air inlet pipe penetrates through the purification box and extends to the top of the purification box, and one end of the air inlet pipe extending to the top of the purification box is communicated with the top of the instrument body.
Preferably, the air outlet intercommunication of air exhauster has the tuber pipe, sliding connection has active carbon adsorption net, compound filter screen and photocatalyst purification net from last to lower in proper order in the both sides of purifying box inner wall.
Preferably, the bottom of the inner wall of the purifying box is fixedly connected with a plasma generator, one side of the front bottom of the purifying box is communicated with an exhaust pipe, and one end of the exhaust pipe is communicated with one side of the instrument body.
Preferably, the top of the inner wall of the instrument body is fixedly connected with an ultraviolet germicidal lamp.
Preferably, the input of air exhauster passes through wire and central processing system's output electric connection, and the output of central processing system passes through the input electric connection of wire and motor, the output of central processing system passes through wire and sterilamp's input electric connection, and the input of sterilamp passes through wire and time relay module's output electric connection, time relay module's input passes through wire and central processing system's output electric connection.
(III) advantageous effects
The invention provides a nucleic acid extractor which can greatly improve the concentration of nucleic acid extraction. The method has the following beneficial effects:
(1) the nucleic acid extraction instrument capable of greatly improving the nucleic acid extraction concentration is characterized in that a purification component is arranged on the back of the top of an instrument body, and a pushing component is arranged in a bottom frame; the pushing assembly comprises a rotating rod, two ends of the rotating rod are rotatably connected with the front surface and the back surface of the inner wall of the bottom frame through bearings, the back surface of the bottom frame is fixedly connected with a motor through a connecting block, one end of an output shaft of the motor is fixedly connected with a first belt pulley through a coupler, one side of the surface of the rotating rod is fixedly connected with a second belt pulley, the surface of the rotating rod is fixedly connected with a rotating sleeve, the surface of the rotating sleeve is provided with an arc-shaped transmission groove, the inner wall of the arc-shaped transmission groove is slidably connected with a sliding rod, the top end of the sliding rod sequentially penetrates through the bottom frame and the instrument body and extends into the instrument body, one end of the sliding rod extending into the instrument body is fixedly connected with the bottom of the test tube rack, the surface of the second belt pulley is in transmission connection with the surface of the first belt pulley, the top of the inner wall of the purifying box is fixedly connected with an exhaust fan through a connecting block, an air inlet of the exhaust fan is communicated with an air inlet pipe, one end of the air inlet pipe penetrates through the purifying box and extends to the top of the purifying box, one end of the air inlet pipe extending to the top of the purifying box is communicated with the top of the instrument body, an air outlet of the exhaust fan is communicated with an air outlet pipe, two sides of the inner wall of the purifying box are sequentially connected with an active carbon adsorption net, a composite filter net and a photocatalyst purifying net in a sliding mode from top to bottom, the bottom of the inner wall of the purifying box is fixedly connected with a plasma generator, one side of the front bottom of the purifying box is communicated with an air exhaust pipe, one end of the air exhaust pipe is communicated with one side of the instrument body, the top of the inner wall of the instrument body is fixedly connected with an ultraviolet sterilizing lamp, avoid operator and the inside contact of extraction appearance, inside simultaneously through the air suction purifying box of air exhauster with the inside air suction of instrument body, inside can carrying the instrument body via inside purification subassembly filtration purification rear, avoid instrument body inside air to breed the bacterium, the quality and the concentration that nucleic acid extracted can have been improved to a certain extent to both cooperations of propelling movement subassembly and purification subassembly, accord with actual user demand.
(2) This improve nucleic acid extraction appearance of nucleic acid extraction concentration by a wide margin, the input through the air exhauster passes through wire and central processing system's output electric connection, and central processing system's output passes through the input electric connection of wire and motor, central processing system's output passes through the input electric connection of wire and sterilamp, and the input of sterilamp passes through the output electric connection of wire and time relay module, the input of time relay module passes through the output electric connection of wire and central processing system, can realize carrying out automation mechanized operation to the mechanical parts on the device, alleviate personnel's working strength.
(3) This improve nucleic acid extraction appearance of nucleic acid extraction concentration by a wide margin, through the bottom fixedly connected with plasma generator of purifying box inner wall, the huge energy release of production in the twinkling of an eye of positive and negative charges neutralization is carried out with the anion to the positive ion that plasma generator produced in the air to lead to the change of its surrounding bacteria structure or the conversion of energy, thereby cause the bacterium death, realize its effect of disinfecting.
Drawings
FIG. 1 is a perspective view of the structure of the present invention;
FIG. 2 is a side view of the internal structure of the bottom frame and the body of the apparatus of the present invention;
FIG. 3 is a rear view of the internal structure of the purification tank of the present invention;
FIG. 4 is a schematic block diagram of the architecture of the system of the present invention;
fig. 5 is a partial enlarged view of the invention at a in fig. 2.
In the figure: 1-bottom frame, 2-instrument body, 3-test tube rack, 4-purification component, 41-purification box, 42-exhaust fan, 43-air inlet pipe, 44-air outlet pipe, 45-activated carbon adsorption net, 46-composite filter screen, 47-photocatalyst purification net, 48-plasma generator, 49-exhaust pipe, 5-pushing component, 51-rotating rod, 52-motor, 53-first belt pulley, 54-second belt pulley, 55-rotating sleeve, 56-arc transmission groove, 57-sliding rod, 6-ultraviolet sterilizing lamp, 7-central processing system, 8-time relay module.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-5, an embodiment of the present invention provides a technical solution: a nucleic acid extractor capable of greatly improving nucleic acid extraction concentration is disclosed, wherein a test tube rack 3 can be automatically pushed into an instrument body 2 to be put into use through the rear part of an ultraviolet germicidal lamp 6, personnel can take the test tube rack 3 outside the instrument to avoid the contact between an operator and the inside of the extractor, meanwhile, air in the instrument body 2 is sucked into a purifying box 41 through an exhaust fan 42, the air is filtered and purified through an internal purifying component 4 and then can be conveyed into the instrument body 2, bacteria breeding in the air in the instrument body 2 is avoided, the quality and concentration of nucleic acid extraction can be improved to a certain extent through the matching of a pushing component 5 and the purifying component 4, the nucleic acid extractor meets the actual use requirement, and comprises a bottom frame 1 and the instrument body 2 fixedly connected to the top of the bottom frame 1, the top of the inner wall of the instrument body 2 is fixedly connected with the ultraviolet germicidal lamp 6, the bottom of the inner wall of the instrument body 2 is slidably connected, the back of the top of the instrument body 2 is provided with a purification component 4, and the inside of the bottom frame 1 is provided with a pushing component 5; the pushing assembly 5 comprises a rotating rod 51, two ends of the rotating rod 51 are rotatably connected with the front surface and the back surface of the inner wall of the bottom frame 1 through bearings, the back surface of the bottom frame 1 is fixedly connected with a motor 52 through a connecting block, one end of an output shaft of the motor 52 is fixedly connected with a first belt pulley 53 through a coupler, one side of the surface of the rotating rod 51 is fixedly connected with a second belt pulley 54, the surface of the second belt pulley 54 is in transmission connection with the surface of the first belt pulley 53 through a belt, the purifying assembly 4 comprises a purifying box 41, the top of the inner wall of the purifying box 41 is fixedly connected with an exhaust fan 42 through a connecting block, an air inlet of the exhaust fan 42 is communicated with an air inlet pipe 43, the input end of the exhaust fan 42 is electrically connected with the output end of the central processing system 7 through a wire, the output end of the central processing system, the working intensity of personnel is reduced, the output end of the central processing system 7 is electrically connected with the input end of the ultraviolet sterilizing lamp 6 through a lead, the input end of the ultraviolet sterilizing lamp 6 is electrically connected with the output end of the time relay module 8 through a lead, the input end of the time relay module 8 is electrically connected with the output end of the central processing system 7 through a lead, the bottom of the inner wall of the purifying box 41 is fixedly connected with a plasma generator 48, positive ions and negative ions generated by the plasma generator 48 generate huge energy release at the moment of neutralizing positive and negative charges in the air, so that the change of the surrounding bacterial structure or the conversion of the energy is caused, bacteria are killed, the sterilizing effect is realized, one side of the front bottom of the purifying box 41 is communicated with an exhaust pipe 49, one end of the exhaust pipe 49 is communicated with one side of the instrument body 2, and the air outlet of the exhaust fan 42 is communicated, the both sides of purifying box 41 inner wall are from last to sliding connection in proper order down having activated carbon adsorption net 45, compound filter screen 46 and photocatalyst purification net 47, the one end of air-supply line 43 runs through purifying box 41 and extends to purifying box 41's top, the one end that air-supply line 43 extends to purifying box 41 top is linked together with instrument body 2's top, purifying box 41's bottom and instrument body 2's top fixed connection, dwang 51's fixed surface is connected with rotates sleeve 55, and rotate sleeve 55's surface and seted up arc-shaped transmission groove 56, and arc-shaped transmission groove 56's inner wall sliding connection has slide bar 57, slide bar 57's top runs through underframe 1 and instrument body 2 in proper order and extends to instrument body 2's inside, slide bar 57 extends to the inside one end of instrument body 2 and the bottom fixed connection of test-tube rack 3.
When in use, firstly, a person opens the movable door on the back of the instrument body 2, places a test tube prepared in advance in the test tube rack 3, turns on the ultraviolet sterilizing lamp 6 to perform sterilization and disinfection treatment on the test tube in advance, the ultraviolet germicidal lamp 6 is timed in advance through the time relay module 8, after the germicidal time reaches the set time, the central processing system 7 controls the motor 52 to start working, the motor 52 rotates forward to drive the rotating rod 51 to start rotating, meanwhile, the rotating sleeve 52 on the rotating rod 51 starts to work, the sliding rod 57 can be driven by the arc-shaped sliding groove 56 to move leftwards along with the rotation of the rotating sleeve 52, and then the sterilized test tube rack 3 is driven to move to the bottom of the extracting part in the instrument body 2 so as to carry out extracting work, and personnel can take and place test tubes outside the instrument body 2 so as to avoid the contact between the personnel and the inside of the instrument body 2;
in the process of extracting nucleic acid, the central processing system 7 controls the exhaust fan 42 to start working, the exhaust fan 42 pumps the air inside the instrument body 2 into the exhaust fan 42 through the air inlet pipe 43, and then the air is conveyed into the purification box 41 through the air outlet pipe 44, the active carbon adsorption net 45 can adsorb partial organic gas in the air, the composite filter net 46 can effectively filter PM2.5 and some fine particles, the photocatalyst purification net 47 can kill germs in the air, the positive ions and the negative ions generated by the plasma generator 48 can generate huge energy release at the moment of neutralizing positive and negative charges in the air, thereby causing the change of the surrounding bacteria structure or the energy conversion, causing the bacteria to die, realizing the sterilization effect, finally conveying the sterilized air to the inside of the instrument body 2 again through the exhaust pipe 49, and thus finishing the whole work.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. The utility model provides an improve nucleic acid extraction appearance of nucleic acid extraction concentration by a wide margin, includes instrument body (2) at underframe (1) and fixed connection bottom frame (1) top, its characterized in that: the bottom of the inner wall of the instrument body (2) is connected with a test tube rack (3) in a sliding mode, a purification assembly (4) is arranged on the back face of the top of the instrument body (2), and a pushing assembly (5) is arranged inside the bottom frame (1);
propelling movement subassembly (5) are including dwang (51), the front and the back rotation that the both ends of dwang (51) all pass through bearing and underframe (1) inner wall are connected, connecting block fixedly connected with motor (52) are passed through at the back of underframe (1) bottom, and the one end of motor (52) output shaft passes through shaft coupling fixedly connected with first belt pulley (53), one side fixedly connected with second belt pulley (54) on dwang (51) surface, the fixed surface of dwang (51) is connected with and rotates sleeve (55), and the surface of rotating sleeve (55) has seted up arc driving groove (56), and the inner wall sliding connection of arc driving groove (56) has slide bar (57), the top of slide bar (57) runs through underframe (1) and instrument body (2) in proper order and extends to the inside of instrument body (2), slide bar (57) extend to the fixed connection in bottom of the inside one end of instrument body (2) and test-tube rack (3) and test tube rack (3) And (6) connecting.
2. The nucleic acid extraction apparatus according to claim 1, wherein the nucleic acid extraction concentration is substantially increased by: the surface of the second belt pulley (54) is in transmission connection with the surface of the first belt pulley (53) through a belt, the purification component (4) comprises a purification box (41), and the bottom of the purification box (41) is fixedly connected with the top of the instrument body (2).
3. The nucleic acid extraction apparatus according to claim 2, wherein the nucleic acid extraction concentration is substantially increased by: the top of purifying box (41) inner wall is through connecting block fixedly connected with air exhauster (42), and the air intake intercommunication of air exhauster (42) has air-supply line (43).
4. The nucleic acid extraction apparatus according to claim 3, wherein the nucleic acid extraction concentration is substantially increased by: one end of the air inlet pipe (43) penetrates through the purification box (41) and extends to the top of the purification box (41), and one end of the air inlet pipe (43) extending to the top of the purification box (41) is communicated with the top of the instrument body (2).
5. The nucleic acid extraction apparatus according to claim 3, wherein the nucleic acid extraction concentration is substantially increased by: the air outlet intercommunication of air exhauster (42) has air-out pipe (44), the both sides of purifying box (41) inner wall have activated carbon adsorption net (45), composite filter net (46) and photocatalyst purification net (47) from last to sliding connection in proper order down.
6. The nucleic acid extraction apparatus according to claim 2, wherein the nucleic acid extraction concentration is substantially increased by: the plasma generator (48) is fixedly connected to the bottom of the inner wall of the purifying box (41), an exhaust pipe (49) is communicated with one side of the front bottom of the purifying box (41), and one end of the exhaust pipe (49) is communicated with one side of the instrument body (2).
7. The nucleic acid extraction apparatus according to claim 1, wherein the nucleic acid extraction concentration is substantially increased by: the top of the inner wall of the instrument body (2) is fixedly connected with an ultraviolet germicidal lamp (6).
8. The nucleic acid extraction apparatus according to claim 3, wherein the nucleic acid extraction concentration is substantially increased by: the input of air exhauster (42) passes through the output electric connection of wire and central processing system (7), and the output of central processing system (7) passes through the input electric connection of wire and motor (52), the input electric connection of wire and sterilamp (6) is passed through to the output of central processing system (7), and the input of sterilamp (6) passes through the output electric connection of wire and time relay module (8), the output electric connection of wire and central processing system (7) is passed through to the input of time relay module (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010789547.XA CN111849745A (en) | 2020-08-07 | 2020-08-07 | Nucleic acid extractor capable of greatly improving nucleic acid extraction concentration |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010789547.XA CN111849745A (en) | 2020-08-07 | 2020-08-07 | Nucleic acid extractor capable of greatly improving nucleic acid extraction concentration |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111849745A true CN111849745A (en) | 2020-10-30 |
Family
ID=72972274
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010789547.XA Pending CN111849745A (en) | 2020-08-07 | 2020-08-07 | Nucleic acid extractor capable of greatly improving nucleic acid extraction concentration |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111849745A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113416630A (en) * | 2021-06-21 | 2021-09-21 | 上海金鹏分析仪器有限公司 | Nucleic acid purification system with anti-pollution effect |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20170253914A1 (en) * | 2014-11-17 | 2017-09-07 | Institute Of Microbiology, Chinese Academy Of Sciences | Apparatus, system, and method for dispensing or mixing micro quantity of liquid |
| CN206902130U (en) * | 2017-07-03 | 2018-01-19 | 广州瑞能医学科技有限公司 | A kind of anti-pollution instrument for extracting nucleic acid |
| CN107962670A (en) * | 2017-12-20 | 2018-04-27 | 中山市雅乐思商住电器有限公司 | Ceramic powder raw material milling and mixing device |
| CN207438762U (en) * | 2017-11-03 | 2018-06-01 | 广东富瑞希空气净化过滤制品有限公司 | A kind of air cleaner |
| CN109306322A (en) * | 2017-07-26 | 2019-02-05 | 上海之江生物科技股份有限公司 | Sample treatment and liquor removing workstation and its application |
| WO2019039629A1 (en) * | 2017-08-24 | 2019-02-28 | (주)구츠 | Air sterilizing/purifying device |
| CN110093343A (en) * | 2019-05-08 | 2019-08-06 | 中国人民解放军军事科学院军事医学研究院 | A kind of integrated portable nucleic acid-extracting apparatus and method for extracting nucleic acid |
| CN210656954U (en) * | 2019-05-28 | 2020-06-02 | 中元牧康(武汉)检测技术服务有限公司 | Full-automatic nucleic acid extraction appearance |
-
2020
- 2020-08-07 CN CN202010789547.XA patent/CN111849745A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20170253914A1 (en) * | 2014-11-17 | 2017-09-07 | Institute Of Microbiology, Chinese Academy Of Sciences | Apparatus, system, and method for dispensing or mixing micro quantity of liquid |
| CN206902130U (en) * | 2017-07-03 | 2018-01-19 | 广州瑞能医学科技有限公司 | A kind of anti-pollution instrument for extracting nucleic acid |
| CN109306322A (en) * | 2017-07-26 | 2019-02-05 | 上海之江生物科技股份有限公司 | Sample treatment and liquor removing workstation and its application |
| WO2019039629A1 (en) * | 2017-08-24 | 2019-02-28 | (주)구츠 | Air sterilizing/purifying device |
| CN207438762U (en) * | 2017-11-03 | 2018-06-01 | 广东富瑞希空气净化过滤制品有限公司 | A kind of air cleaner |
| CN107962670A (en) * | 2017-12-20 | 2018-04-27 | 中山市雅乐思商住电器有限公司 | Ceramic powder raw material milling and mixing device |
| CN110093343A (en) * | 2019-05-08 | 2019-08-06 | 中国人民解放军军事科学院军事医学研究院 | A kind of integrated portable nucleic acid-extracting apparatus and method for extracting nucleic acid |
| CN210656954U (en) * | 2019-05-28 | 2020-06-02 | 中元牧康(武汉)检测技术服务有限公司 | Full-automatic nucleic acid extraction appearance |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113416630A (en) * | 2021-06-21 | 2021-09-21 | 上海金鹏分析仪器有限公司 | Nucleic acid purification system with anti-pollution effect |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111849745A (en) | Nucleic acid extractor capable of greatly improving nucleic acid extraction concentration | |
| CN115057566B (en) | Chemical wastewater purification treatment instrument | |
| CN115403232A (en) | Turnover type excrement treatment device and method for pig breeding | |
| JP2021534751A (en) | Pathogen concentration and nucleic acid extraction method using on-site diagnostic equipment | |
| CN109011867B (en) | Prevent medicine production of pollution with sterilization clean room | |
| CN204039215U (en) | Integrated sewage treatment recycling device | |
| CN208574038U (en) | A kind of Hepatosis blood sample processing unit | |
| CN109045314A (en) | Operating room integrates decontamination system, packaging technology and comprehensive sterilization method in advance | |
| CN212068358U (en) | Novel reverse osmosis device | |
| CN106746083A (en) | A kind of rice processing water purification installation | |
| CN113582404A (en) | Clinical laboratory uses medical waste liquid degassing unit | |
| CN210193840U (en) | Nucleic acid extraction and purification device | |
| CN220142967U (en) | Ultrasonic inactivation treatment device for wastewater | |
| CN218290915U (en) | Full-automatic nucleic acid extraction appearance | |
| CN216537704U (en) | EO sterilization back exhaust treatment device | |
| CN212393763U (en) | Food sterilization device convenient for removing peculiar smell | |
| CN113564025B (en) | Microorganism disinfection treatment equipment for wine fermentation | |
| CN217895358U (en) | Water purifier with sterilization and virus killing functions | |
| CN215288364U (en) | Medical waste treatment's sewage treatment device | |
| CN117463081B (en) | Nonferrous metal smelting waste gas separation device | |
| CN215310831U (en) | Energy-saving and environment-friendly device for recycling waste electrolytic copper | |
| CN213447070U (en) | Internal circulation device for extracting large-flux nucleic acid | |
| CN216916744U (en) | Vegetable fresh-keeping cabinet with sterilizing device | |
| CN214400493U (en) | Pet treatment antibody preparation culture apparatus | |
| CN116379600A (en) | Ventilation method and device for biological medicine test |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20201030 |
|
| RJ01 | Rejection of invention patent application after publication |