CN210193840U

CN210193840U - Nucleic acid extraction and purification device

Info

Publication number: CN210193840U
Application number: CN201920873245.3U
Authority: CN
Inventors: Xiaodong Han; 韩小东; Jing Liu; 刘敬; Jianxiang Cheng; 程建祥
Original assignee: Shandong Sikejie Scientific Instrument Co Ltd
Current assignee: Shandong Sikejie Scientific Instrument Co Ltd
Priority date: 2019-06-10
Filing date: 2019-06-10
Publication date: 2020-03-27
Anticipated expiration: 2029-06-10

Abstract

The utility model discloses a nucleic acid extraction and purification device, the on-line screen storage device comprises a base, base top fixedly connected with seal box, bottom central authorities position fixedly connected with collection liquid box in the seal box, bottom left side rotates through first servo motor in the seal box and is connected with the support, the support top has cup jointed first collecting pipe through first bottle stopper, first collecting pipe passes through slider sliding connection and is inboard in the slide rail of seal box top middle part workstation upper end central point position installation, and has the second collecting pipe through slider sliding connection on one side of the first collecting pipe, workstation upper end both sides fixedly connected with mounting bracket, and mounting bracket one side fixedly connected with mounting panel, mounting bracket top one side fixedly connected with second servo motor. The utility model discloses a set up time-recorder, temperature sensor, guiding gutter, collection liquid case, ultraviolet tube and air inlet structure, have the advantage that improves nucleic acid and draw purification efficiency, avoid the waste liquid pollution.

Description

Nucleic acid extraction and purification device

Technical Field

The utility model relates to a nucleic acid extraction technical field specifically is a nucleic acid extraction purification device.

Background

Nucleic acid is a biological macromolecular compound formed by polymerizing a plurality of nucleotides, is one of the most basic substances of life, and is widely existed in all animal and plant cells and microorganisms, nucleic acid in organisms is often combined with protein to form nucleoprotein, different nucleic acids are different in chemical composition, nucleotide arrangement sequence and the like, the nucleic acid can be divided into ribonucleic acid and deoxyribonucleic acid according to the difference of the chemical composition, and the extraction method of the nucleic acid by a general device comprises the following steps: DND extraction and purification method and RNA extraction and purification method.

However, the prior art has the following defects:

1. in the process of extracting nucleic acid by the existing device, time and temperature cannot be effectively controlled, so that the purification efficiency is reduced, and the solution is not easy to guide;

2. in the process of extracting nucleic acid by using the conventional device, the generated waste liquid is directly discharged without being treated, so that the environmental pollution is easily caused.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

The utility model provides a not enough to prior art, the utility model provides a nucleic acid extraction and purification device has solved the in-process that exists current device and draw nucleic acid among the prior art, can't carry out effectual control to time and temperature, causes the reduction of purification efficiency, and solution is difficult for carrying out the water conservancy diversion, and the waste liquid of production directly discharges through not handling, easily causes the problem of the pollution of environment.

(II) technical scheme

In order to achieve the above object, the utility model provides a following technical scheme: a nucleic acid extraction and purification device comprises a base, wherein a sealing box is fixedly connected to the top end of the base, an ultraviolet lamp tube is installed on one side inside the sealing box and one side of the inner top end of the sealing box, a liquid collecting box is fixedly connected to the central position of the inner bottom end of the sealing box, a support is rotatably connected to the left side of the inner bottom end of the sealing box through a first servo motor and is connected with the first servo motor through a bearing plate, a first collecting pipe is sleeved at the top of the support through a first bottle plug and is slidably connected to the inner side of a sliding rail installed at the central position of the upper end of a workbench in the middle of the top end of the sealing box through a sliding block, a second collecting pipe is slidably connected to one side of the first collecting pipe through a sliding block, a liquid outlet is formed in the middle of the bottom end of the workbench, mounting frames are fixedly, mounting bracket top one side fixedly connected with second servo motor, second servo motor rotates through the shaft coupling and is connected with the axostylus axostyle, the time-recorder has been inlayed in axostylus axostyle surface middle section, and the centrifuging tube has been cup jointed through the second bottle plug in the axostylus axostyle bottom, the bottom both sides are provided with the heating plate in the centrifuging tube, first bottle plug is inlayed with the inboard middle section of second bottle plug and is had temperature sensor.

Preferably, the inside both sides lateral wall of centrifuging tube equidistance is provided with the guiding gutter, and centrifuging tube positive surface lower part central point threaded connection has the plug screw.

Preferably, the middle part of the outer side of the sliding block is fixedly connected with a supporting rod through a connecting rod, and the supporting rod is movably connected with the output ends of the left hydraulic cylinder and the right hydraulic cylinder which are arranged on two sides in the sliding rail.

Preferably, the display screen and the operating button are sequentially inlaid on two sides of the upper part of the front surface of the mounting plate, and an indicating lamp is arranged between the display screen and the operating button.

Preferably, the central positions of the upper part and the lower part of the front surface of the liquid collecting box are respectively provided with an air inlet and a water outlet, and the inner threads of the air inlet and the water outlet are connected with bolts.

Preferably, the middle section of the inner side of the support is fixedly connected with a telescopic rod through a transverse plate, and the top of the telescopic rod is in threaded connection with an adsorption column arranged in the middle of the first collecting pipe.

(III) advantageous effects

The utility model provides a nucleic acid extraction and purification device possesses following beneficial effect:

(1) the utility model has the advantages that the timer, the temperature sensor and the diversion trench are arranged, the effect of improving the nucleic acid extraction and purification efficiency is achieved, the problems that the time and the temperature cannot be effectively controlled in the nucleic acid extraction process of the prior device, the purification efficiency is reduced, and the solution is not easy to conduct diversion are solved, the timer is embedded at the central position of the front surface of the shaft rod, when the first collecting pipe and the centrifuge pipe are centrifugally operated by the first servo motor and the second servo motor, the accurate centrifugal time calculation is carried out by the timer, the temperature sensor is embedded at the central position of the inner sides of the first bottle plug and the second bottle plug, the heating temperature inside the centrifuge pipe is controlled in real time by the temperature sensor, once the temperature reaches the designated temperature, the controller stops the work of the heating sheet, and realizes the real-time control of the temperature inside the centrifuge pipe, simultaneously, be provided with the guiding gutter in the inboard both sides lateral wall of centrifuging tube, the liquid after the centrifugation of being convenient for carries out the water conservancy diversion operation, helps improving the efficiency of nucleic acid extraction purification.

(2) The utility model has the advantages that the liquid collecting box, the ultraviolet lamp tube and the air inlet are arranged, the liquid waste can be conveniently collected and the discharged liquid can be treated, the problem that the generated liquid waste is directly discharged without treatment and environmental pollution is easily caused in the process of extracting nucleic acid by the existing device is solved, the liquid outlet is arranged at the central position of the bottom end of the worktable, the liquid outlet and the liquid collecting box are oppositely arranged, the generated liquid waste enters the liquid collecting box from the liquid outlet to be collected in the process of extracting and purifying nucleic acid by the device, the ultraviolet lamp tube is arranged on the inner wall of the sealing box outside the liquid collecting box to disinfect and degerming the liquid waste in the liquid collecting box, meanwhile, the air inlet is arranged at the middle section of the upper part of the front surface of the liquid collecting box, ozone is input from the air inlet to disinfect the liquid waste again, the bolt is unscrewed to discharge the liquid waste, causing environmental pollution.

Drawings

FIG. 1 is a front view of the present invention;

FIG. 2 is a schematic view of the inner structure of the centrifugal tube of the present invention;

fig. 3 is a schematic top view of the slide rail of the present invention.

The reference numbers in the figures are: 1. a base; 2. a first servo motor; 3. a bearing plate; 4. a water outlet; 5. a bolt; 6. an air inlet; 7. a liquid collection tank; 8. a sealing box; 9. an ultraviolet lamp tube; 10. centrifuging the tube; 11. an adsorption column; 12. a first stopper; 13. a telescopic rod; 14. a support; 15. a transverse plate; 16. a mounting frame; 17. a second servo motor; 18. a coupling; 19. a liquid outlet; 20. a second collection tube; 21. a display screen; 22. an indicator light; 23. mounting a plate; 24. an operation button; 25. a controller; 26. a work table; 27. a shaft lever; 28. a temperature sensor; 29. a plug screw; 30. a timer; 31. a second stopper; 32. a diversion trench; 33. a heating plate; 34. a left hydraulic cylinder; 35. a strut; 36. a slide rail; 37. a connecting rod; 38. a first collection tube; 39. a slider; 40. and a right hydraulic cylinder.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

As shown in fig. 1-3, the utility model provides a technical solution: the utility model provides a nucleic acid extraction purification device, which comprises a base 1, 1 top fixedly connected with seal box 8 of base, ultraviolet tube 9 is installed with interior top one side to the inside one side of seal box 8, 8 interior bottom central authorities of seal box position fixedly connected with collection liquid box 7, collection liquid box 7 is on the front surface, air inlet 6 and delivery port 4 have been seted up respectively to lower part central authorities position, and air inlet 6 and 4 internal thread of delivery port are connected with bolt 5, 8 interior bottom left sides of seal box rotate through first servo motor 2 and are connected with support 14, and support 14 is connected with first servo motor 2 through accepting board 3, the inboard middle section of support 14 passes through diaphragm 15 fixedly connected with telescopic link 13, and the adsorption column 11 threaded connection of middle part setting in telescopic link 13 top and first collecting pipe 38, support 14 top has cup jointed first collecting pipe 38 through first bottle plug 12, first collecting pipe 38 passes through slide rail 36 of slider 39 sliding connection in the installation of the middle part position of seal box 8 top middle part workstation 26 Inboard, and first collecting pipe 38 one side has second collecting pipe 20 through slider 39 sliding connection, workstation 26 bottom middle part is provided with liquid outlet 19, position in workstation 26 bottom central point is provided with liquid outlet 19, liquid outlet 19 sets up with collecting tank 7 relatively, the device is in the in-process of extracting the purification to nucleic acid, the waste liquid that produces gets into collecting tank 7 inside by liquid outlet 19 and is collected, and install ultraviolet tube 9 in the seal box 8 inner wall outside collecting tank 7, carry out the virus killing and bacterium removing to the waste liquid in collecting tank 7, simultaneously, set up air inlet 6 in collecting tank 7 positive surface upper portion middle section, throw in ozone by air inlet 6, carry out the secondary sterilization to the waste liquid, and unscrew bolt 5 and discharge the waste liquid by delivery port 4, avoid directly discharging the waste liquid, cause the pollution of environment, slider 39 outside middle part is through connecting rod 37 fixedly connected with branch 35, the support rod 35 is movably connected with the output ends of the left hydraulic cylinder 34 and the right hydraulic cylinder 40 which are arranged on two sides inside the slide rail 36, the mounting frame 16 is fixedly connected with two sides of the upper end of the workbench 26, the mounting plate 23 is fixedly connected with one side of the mounting frame 16, the controller 25 is arranged on the lower portion of the front surface of the mounting plate 23, the model of the controller 25 is RS485, the mounting frame belongs to the prior art and is used for controlling the first servo motor 2, the second servo motor 17, the temperature sensor 28, the timer 30 and the heating sheet 33, the display screen 21 and the operation button 24 are sequentially embedded on two sides of the upper portion of the front surface of the mounting plate 23, the indicator lamp 22 is arranged between the display screen 21 and the operation button 24, the second servo motor 17 is fixedly connected with one side of the top end of the mounting frame 16, the model of the first servo motor 2 and the second servo motor 17 is Y2100, the middle section of the surface of the shaft rod 27 is embedded with a timer 30, the bottom end of the shaft rod 27 is sleeved with the centrifugal tube 10 through a second bottle stopper 31, the model of the timer 30 is SST10, the centrifugal tube 10 belongs to the prior art, both sides of the bottom end of the centrifugal tube 10 are provided with heating sheets 33, the model of the heating sheets 33 is MZ9280C, the centrifugal tube 10 belongs to the prior art, both side walls of the interior of the centrifugal tube 10 are provided with diversion trenches 32 at equal intervals, the central position of the lower part of the front surface of the centrifugal tube 10 is connected with a screw plug 29 in a threaded manner, the middle sections of the inner sides of the first bottle plug 12 and the second bottle stopper 31 are embedded with a temperature sensor 28, the model of the temperature sensor 28 is PT100, the prior art, the central position of the front surface of the shaft rod 27 is embedded with the timer 30, when the first servo motor 2 and the second servo motor 17 are used for carrying out centrifugal operation on the first collecting tube 38 and the centrifugal, control the inside heating temperature of centrifuging tube 10 through temperature sensor 28 real-time, in case when the temperature reaches the assigned temperature, controller 25 stops heating plate 33 work, realizes the real-time control to the inside temperature of centrifuging tube 10, simultaneously, is provided with guiding gutter 32 at the inboard both sides lateral wall of centrifuging tube 10, and the liquid after the centrifugation of being convenient for carries out the water conservancy diversion operation, helps improving the efficiency that nucleic acid drawed the purification.

The working principle is as follows: when the device is used, the device is powered on, the indicator lamp 22 is turned on, timing and temperature fixing operations are carried out through the operation buttons 24 on the mounting plate 23, the display screen 21 displays that the sealing box 8 is mounted by using the base 1, a sample and digestive juice are added into the centrifugal tube 10 on one side of the upper end of the workbench 26, the second servo motor 17 on one side of the top of the mounting frame 16 rotates the shaft rod 27 through the coupler 18, the second bottle stopper 31 at the bottom end of the shaft rod 27 seals the centrifugal tube 10, under the rotation of the second servo motor 17, the controller 25 controls the timer 30 and the temperature sensor 28 to ensure the heating temperature and the centrifugal time of the heating sheet 33 inside the centrifugal tube 10, after the centrifugal tube 10 is subjected to short centrifugation, absolute ethyl alcohol is added for carrying out secondary short centrifugation on the sample, the diversion groove 32 is arranged on the inner side of the centrifugal tube 10, the screw plug 29 is unscrewed, so that the centrifuged solution enters the first collection tube 38, at this time, the washing liquid is added into the first collecting pipe 38, the first servo motor 2 rotates the bracket 14 through the bearing plate 3, the first bottle stopper 12 at the top of the bracket 14 seals the first collecting pipe 38, the first servo motor 2 rotates the first collecting pipe 38, the solution is absorbed by the absorption column 11, after the first collecting pipe 38 is centrifuged, the solution enters the liquid collecting tank 7 from the liquid outlet 19 and is collected, the ultraviolet lamp tube 9 is installed on the inner wall of the sealing tank 8 outside the liquid collecting tank 7, the waste liquid in the liquid collecting tank 7 is sterilized and disinfected, at the same time, the air inlet 6 is opened in the middle section of the upper surface of the liquid collecting tank 7, ozone is injected from the air inlet 6 to sterilize the waste liquid again, the bolt 5 is opened, the waste liquid is discharged from the water outlet 4, the direct discharge of the waste liquid is avoided, the pollution to the environment is caused, at this time, the telescopic rod 13 at the upper end of the transverse plate 15 descends the absorption column, left pneumatic cylinder 34 carries out the contraction action through branch 35 to connecting rod 37, make slider 39 carry out the removal left in slide rail 36 inboard, and then drive first collecting pipe 38 and remove left, simultaneously, right pneumatic cylinder 40 is to carrying out the elongation operation to connecting rod 37 through branch 35, make slider 39 drive second collecting pipe 20 carry out the motion left, make second collecting pipe 20 reach first bottle stopper 12 top, telescopic link 13 carries out the operation that rises to adsorption column 11, make adsorption column 11 get into in the second collecting pipe 20, and to adding sterile water in the second collecting pipe 20, utilize first servo motor 2 to carry out centrifugal action to second collecting pipe 20, the realization is to the extraction purification of nucleic acid.

To sum up can, the utility model discloses a set up time-recorder 30, temperature sensor 28, guiding gutter 32, header tank 7,

ultraviolet tube

9 and 6 structures of air inlet, solved and to have the in-process that prior device carries out extraction to nucleic acid among the prior art, can't carry out effectual accuse to time and temperature, cause the reduction of purification efficiency, and solution is difficult for carrying out the water conservancy diversion, and the waste liquid of production directly discharges through the processing, easily causes the problem of the pollution of environment.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A nucleic acid extraction and purification device comprises a base (1), and is characterized in that: the ultraviolet lamp tube collecting device is characterized in that a sealing box (8) is fixedly connected to the top end of the base (1), an ultraviolet lamp tube (9) is installed on one side of the inner portion and one side of the inner top end of the sealing box (8), a liquid collecting box (7) is fixedly connected to the central position of the inner bottom end of the sealing box (8), a support (14) is rotatably connected to the left side of the inner bottom end of the sealing box (8) through a first servo motor (2), the support (14) is connected with the first servo motor (2) through a bearing plate (3), a first collecting pipe (38) is sleeved on the top of the support (14) through a first bottle stopper (12), the first collecting pipe (38) is slidably connected to the inner side of a sliding rail (36) installed at the central position of the upper end of a middle worktable (26) of the top end of the sealing box (8) through a sliding block (39), and a, workstation (26) bottom middle part is provided with liquid outlet (19), workstation (26) upper end both sides fixedly connected with mounting bracket (16), and mounting bracket (16) one side fixedly connected with mounting panel (23), mounting panel (23) front surface lower part is provided with controller (25), mounting bracket (16) top one side fixedly connected with second servo motor (17), second servo motor (17) are rotated through shaft coupling (18) and are connected with axostylus axostyle (27), axostylus axostyle (27) middle section is inlayed and is had time-recorder (30), and axostylus axostyle (27) bottom has cup jointed centrifuging tube (10) through second bottle plug (31), bottom both sides are provided with heating plate (33) in centrifuging tube (10), first bottle plug (12) are inlayed with temperature sensor (28) in second middle section (31) inboard.

2. The nucleic acid extraction and purification device according to claim 1, wherein: the inside both sides lateral wall of centrifuging tube (10) equidistance is provided with guiding gutter (32), and centrifuging tube (10) positive surface lower part central point threaded connection has plug screw (29).

3. The nucleic acid extraction and purification device according to claim 1, wherein: the middle part of the outer side of the sliding block (39) is fixedly connected with a support rod (35) through a connecting rod (37), and the support rod (35) is movably connected with the output ends of a left hydraulic cylinder (34) and a right hydraulic cylinder (40) which are arranged on two sides in the sliding rail (36).

4. The nucleic acid extraction and purification device according to claim 1, wherein: the utility model discloses a display screen (21) and operating button (24) have been inlayed in proper order to mounting panel (23) positive surface upper portion both sides, and are provided with pilot lamp (22) between display screen (21) and operating button (24).

5. The nucleic acid extraction and purification device according to claim 1, wherein: the upper and lower central positions of the front surface of the liquid collecting box (7) are respectively provided with an air inlet (6) and a water outlet (4), and the air inlet (6) and the water outlet (4) are internally connected with bolts (5) through threads.

6. The nucleic acid extraction and purification device according to claim 1, wherein: the middle section of the inner side of the bracket (14) is fixedly connected with a telescopic rod (13) through a transverse plate (15), and the top of the telescopic rod (13) is in threaded connection with an adsorption column (11) arranged in the middle of the inside of the first collecting pipe (38).