CN216404407U

CN216404407U - Automatic equipment for extracting large-flux nucleic acid

Info

Publication number: CN216404407U
Application number: CN202122844429.7U
Authority: CN
Inventors: 丁小静
Original assignee: Jiangsu Uninovo Biological Technology Co ltd
Current assignee: Jiangsu Uninovo Biological Technology Co ltd
Priority date: 2021-11-19
Filing date: 2021-11-19
Publication date: 2022-04-29
Anticipated expiration: 2031-11-19

Abstract

The invention discloses an automatic device for extracting high-flux nucleic acid, which belongs to the technical field of biochemistry. The motion assembly comprises a platform assembly, an experiment cabin assembly and a Z shaft assembly. The bottom plate assembly comprises a base plate assembly, a fan mounting plate, feet and a fan. The circuit mounting assembly comprises a circuit mounting plate, a switching power supply, a circuit main board, a filter and a terminal strip. The housing assembly includes a panel assembly, an integral housing, a screen assembly, a screen mount, and a back plate. The nucleic acid extraction experiment is carried out through the embodiment, the repeatability and the working efficiency of the experiment are improved, and the method can be used for extracting large-flux samples at the same time.

Description

Automatic equipment for extracting large-flux nucleic acid

Technical Field

The invention relates to the technical field of biochemistry, in particular to an automatic device for extracting large-flux nucleic acid.

Background

The extraction technology of nucleic acid molecules is a key technology of molecular biology research, and the nucleic acid amplification technology can be used for plant pathogenic organism detection, food detection and disease diagnosis, and has very important significance. Generally, nucleic acid extraction requires multiple steps, and firstly, biological sample materials such as cells and tissue materials need to be subjected to a disruption treatment, nuclease inactivation, nucleic acid release, and then other tissue or cell components such as proteins, polysaccharides, lipids, and the like, so as to obtain high-quality nucleic acid. Since 1869 Swiss physician Friedrich Miescher first extracted DNA from cells until the 90's last century, nucleic acid extraction has been a time-consuming task that required the use of toxic reagents. The traditional nucleic acid extraction technology is mostly manually carried out by workers, more than ten reagents and multiple processes are designed, the operation is complex, the working efficiency is low, and the repeatability of manual operation instability experiments is poor.

Disclosure of Invention

The application provides an automation equipment for large-flux nucleic acid extraction for solve the problems of low working efficiency of artificial nucleic acid extraction and poor experimental repeatability.

The application provides an automation equipment for big flux nucleic acid draws, its characterized in that includes: a moving component, a chassis component, a circuit mounting component, a housing component, a backplane, and the like.

Furthermore, the motion assembly comprises a platform assembly, an experiment cabin assembly and a Z shaft assembly.

Furthermore, the bottom plate assembly comprises a base plate assembly, a fan mounting plate, feet and a fan.

Furthermore, the circuit mounting assembly comprises a circuit mounting plate, a switching power supply, a circuit main board, a filter and a terminal strip.

Further, the shell assembly comprises a panel assembly, an integral shell, a screen assembly, a screen mounting seat and a back plate.

Furthermore, the bottom of the platform assembly is a platform bottom plate, the front part of the platform bottom plate is surrounded by a pore plate isolation strip and a pore plate stop block to form a deep pore plate mounting groove, and the heating block assembly is arranged in the mounting groove and used for heating the bottom of the deep pore plate. The rear part of the platform bottom plate is provided with a driving wheel component and an idle wheel component which are connected through a conveyor belt, and the conveyor belt can drive the platform connecting plate to move along the horizontal shaft.

Furthermore, an ultraviolet lamp assembly is arranged at the top in the experiment cabin assembly cabin, and fan assemblies are arranged on two sides of the cabin body and used for ensuring the cleanliness in the experiment cabin before and after nucleic acid extraction and in the extraction process.

Furthermore, the Z-axis component drives the sliding block connecting plate on the Z-axis guide rail to move through the Hayton motor, so that the magnetic rod connecting plate and the magnetic sleeve connecting plate move up and down to carry out operations such as uniform mixing and washing of reagents in the deep hole plate. Meanwhile, the magnetic bar connecting plate is separated from the magnetic sleeve connecting plate through the magnetic bar motor, and magnetic beads are separated from the magnetic bars.

The beneficial effect of this application:

the automatic equipment for extracting the large-flux nucleic acid provided by the embodiment comprises a moving assembly, a bottom plate assembly, a circuit mounting assembly, a shell assembly, a back plate and the like. When a nucleic acid extraction experiment is required, a matched nucleic acid extraction reagent is placed in a deep hole plate placing groove of a platform bottom plate at the bottom of the platform assembly, and the heating of the extraction reagent is realized by a heating block assembly arranged in the placing groove. The Hayton motor in the Z-axis component drives the sliding block connecting plate on the Z-axis guide rail to move, so that the magnetic rod and the magnetic sleeve move up and down to carry out operations such as mixing and washing of the reagent in the deep hole plate. The magnetic bar connecting plate and the magnetic sleeve connecting plate are separated through the magnetic bar motor, and the magnetic bead adsorption and release operation is realized. The rear part of the platform bottom plate is provided with a driving wheel component and an idle wheel component which are connected through a conveyor belt, and the conveyor belt can drive the magnetic rod and the magnetic sleeve to move along the horizontal axis, so that the purpose of transferring magnetic beads among the deep hole plate rows is realized. The nucleic acid extraction experiment is carried out through the embodiment, the repeatability and the working efficiency of the experiment are improved, and the method can be used for extracting large-flux samples at the same time.

Drawings

FIG. 1 is a schematic diagram of the structure of an apparatus according to an embodiment of the present application;

FIG. 2 is a schematic diagram of the internal structure of an apparatus from another perspective in one embodiment of the present application;

FIG. 3 is a schematic diagram of a kinematic assembly of the apparatus according to one embodiment of the present application;

FIG. 4 is a schematic view of a motion assembly from another perspective of an apparatus according to an embodiment of the present disclosure;

FIG. 5 is a schematic diagram of a circuit mounting assembly of the apparatus according to an embodiment of the present application;

in the figure: 11. a platform assembly; 12. an experiment compartment assembly; 14. a Z-axis assembly; 21. a substrate assembly; 23. ground feet; 24. a fan; 31. a circuit mounting board; 32. a switching power supply; 33. a circuit main board; 34. a filter; 35. a terminal block; 41. a panel assembly; 42. an integral housing; 43. a screen assembly; 5. a back plate.

Detailed Description

The present application is described in further detail in the following detailed description of the preferred embodiments with reference to the figures, in which like elements in different embodiments are numbered with like associated element numbers. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).

The embodiment provides an automatic device for extracting large-flux nucleic acid.

Referring to fig. 1-5, the device includes a moving assembly (1), a chassis assembly (2), a circuit mounting assembly (3), a housing assembly (4), a back plate (5), and so on.

Referring to FIGS. 1-2, when nucleic acid extraction is required, the main power supply (32) of the apparatus is first turned on, the control software on the screen assembly (43) turns on the UV lamp to perform pre-experiment sterilization, and after the sterilization is completed, the panel (41) is opened to place the extraction kit in the well plate receiving slot of the platform assembly (11) in the experiment chamber (12) to ensure that the bottom of the well is in full contact with the heating block. After the magnetic sleeve connecting plate is provided with the magnetic rod sleeve, the panel is closed, and the nucleic acid extraction program is started to run. After extraction is started, a driving wheel and an idler wheel at the rear part of the platform (11) drive a Z shaft assembly (14) to horizontally move through a conveyor belt, so that the magnetic rod and the magnetic rod sleeve move to the positions above the corresponding reagent deep holes, and then heating of the extracted reagent is realized through a heating block assembly arranged in the mounting groove. After heating begins, a Hayton motor in the Z-axis assembly drives a sliding block connecting plate on the Z-axis guide rail to move, so that the magnetic rod and the magnetic sleeve move up and down to carry out operations such as mixing and washing of reagents in the deep hole plate. The magnetic bar motor realizes the separation of the magnetic bar connecting plate and the magnetic sleeve connecting plate and realizes the adsorption and release operation of magnetic beads.

The foregoing is a more detailed description of the present application in connection with specific embodiments thereof, and it is not intended that the present application be limited to the specific embodiments thereof. It will be apparent to those skilled in the art from this disclosure that many more simple derivations or substitutions can be made without departing from the inventive concepts herein.

Claims

1. An automated apparatus for high flux nucleic acid extraction, comprising: the device comprises a motion component (1), a bottom plate component (2), a circuit installation component (3), a shell component (4), a back plate (5) and the like;

the motion assembly (1) comprises a platform assembly (11), an experiment cabin assembly (12) and a Z shaft assembly (14);

the bottom plate component (2) comprises a base plate component (21), a fan mounting plate (22), feet (23) and a fan (24);

the circuit mounting assembly (3) comprises a circuit mounting plate (31), a switching power supply (32), a circuit main board (33), a filter (34) and a terminal strip (35).

2. The shell assembly (4) comprises a panel assembly (41), an integral shell (42), a screen assembly (43), a screen mounting seat (44) and a back plate (5); the automatic equipment for large-flux nucleic acid extraction is characterized in that the bottom of the platform assembly (11) is a platform bottom plate, the front part of the platform bottom plate is surrounded by a pore plate isolation strip and a pore plate stop block to form a deep pore plate placing groove, and a heating block assembly for heating the bottom of the deep pore plate is arranged in the placing groove; the rear part of the platform bottom plate is provided with a driving wheel component and an idle wheel component which are connected through a conveyor belt, and the conveyor belt can drive the platform connecting plate to move along the horizontal shaft.

3. The automated equipment for high-flux nucleic acid extraction according to claim 1, wherein the top of the inside of the experiment chamber assembly (12) is provided with an ultraviolet lamp assembly, and the two sides of the chamber body are provided with fan assemblies for ensuring the cleanliness of the inside of the experiment chamber before and after nucleic acid extraction and during the extraction process.

4. The automation equipment for large-flux nucleic acid extraction according to claim 1, wherein the Z-axis assembly (14) drives a sliding block connecting plate on a Z-axis guide rail to move through a Hayton motor, so that the magnetic rod connecting plate and the magnetic sleeve connecting plate move up and down to carry out operations such as uniform mixing and washing of reagents in the deep-hole plate; meanwhile, the magnetic bar connecting plate is separated from the magnetic sleeve connecting plate through the magnetic bar motor, and magnetic beads are separated from the magnetic bars.