CN218710538U - Magnetic sleeve frame assembly, magnetic attraction mechanism and sample extraction equipment - Google Patents

Abstract

The application provides a mechanism and sample extraction equipment are inhaled to magnetic sleeve frame subassembly, magnetism, and magnetic sleeve frame subassembly includes: the magnetic sleeve mounting plate comprises a magnetic sleeve mounting plate and a plurality of magnetic sleeve assemblies; be equipped with the magnetic sleeve installing zone on the magnetic sleeve mounting panel, the magnetic sleeve installing zone is provided with a plurality of mounting grooves along the first direction interval, and arbitrary mounting groove in a plurality of mounting grooves extends along the second direction, and a plurality of magnetic sleeve subassemblies are pegged graft respectively in the mounting groove that corresponds in a plurality of mounting grooves, and a plurality of mounting grooves are configured to make a plurality of magnetic sleeve subassemblies have two kind at least modes of arranging. The magnetic sleeve frame assembly is flexible in configuration and high in space utilization rate, and the extraction flux and the extraction efficiency of the sample extraction equipment are improved.

Description

Magnetic sleeve frame assembly, magnetic attraction mechanism and sample extraction equipment

Technical Field

The application relates to the technical field of nucleic acid extraction, especially, relate to a magnetic sleeve frame subassembly, magnetism inhale mechanism and sample and draw equipment.

Background

Nucleic acid extraction is one of the most basic experiments in molecular experiments, and is the basis of almost all experiments, and nucleic acid can be successfully performed no matter subsequent cloning, polymerase Chain Reaction (PCR), quantitative real-time Polymerase Chain Reaction (Q-PCR), library construction and sequencing, and the like.

At present, nucleic acid extraction equipment of market mainstream mainly uses the magnetic bead method to purify nucleic acid, the deep hole board that contains the sample is placed on nucleic acid extraction equipment's extraction bench, nucleic acid extraction equipment's magnetism inhale the mechanism and set up the magnetic sleeve frame in the supporting below bar magnet frame, the bar magnet frame moves down every bar magnet on it all inserts in every magnetic sleeve on the magnetic sleeve frame, adsorb the magnetic bead in the magnetic sleeve bottom through the motion of bar magnet and magnetic sleeve, and shift in different reaction reagent, utilize the high frequency of bar magnet and magnetic sleeve to reciprocate the stirring reagent simultaneously, through steps such as cell lysis, nucleic acid adsorption, washing, elution, extract high-purity nucleic acid.

However, in the existing nucleic acid extraction equipment, the configuration of the magnetic attraction mechanism is fixed, the space utilization rate is low, and the test flux is limited.

SUMMERY OF THE UTILITY MODEL

In order to solve at least one problem mentioned in the background art, the application provides a magnetic sleeve frame assembly, a magnetic suction mechanism and sample extraction equipment.

In a first aspect, the present application provides a magnetic carriage assembly comprising: the magnetic sleeve mounting plate comprises a magnetic sleeve mounting plate and a plurality of magnetic sleeve assemblies;

be equipped with the magnetic sleeve installing zone on the magnetic sleeve mounting panel, the magnetic sleeve installing zone is provided with a plurality of mounting grooves along first direction interval, and arbitrary mounting groove in a plurality of mounting grooves extends along the second direction, and a plurality of magnetic sleeve subassemblies are pegged graft respectively in the mounting groove that corresponds in a plurality of mounting grooves, and a plurality of mounting grooves are configured to make a plurality of magnetic sleeve subassemblies have two kind at least modes of arranging.

In a possible implementation mode, one end of the installation groove is an insertion end, and the insertion end is communicated to the end face of the magnetic sleeve installation plate.

In one possible embodiment, the magnetic sleeve frame assembly further comprises a blocking piece, and the blocking piece is detachably inserted into the insertion end of the installation groove.

In a possible embodiment, the blocking piece comprises a blocking portion and a holding portion, the blocking portion is inserted into the mounting groove, and the holding portion extends out of the mounting groove.

In a possible embodiment, the magnetic sleeve mounting area is further provided with a plurality of communication grooves at intervals along the first direction, and any one of the communication grooves extends along the second direction; the communicating groove corresponds to the mounting groove one by one, and the two sides of the depth direction of the communicating groove are communicated with the top surface and the mounting groove of the magnetic sleeve mounting plate respectively.

In a possible embodiment, the width of the communication groove is smaller than the width of the mounting groove.

In a possible implementation manner, the magnetic sleeve assembly comprises a fixing plate and a plurality of magnetic sleeves, the fixing plate is detachably inserted into the mounting groove, and the magnetic sleeves are connected to the fixing plate and are sequentially arranged along the second direction.

In a possible implementation mode, the fixing plate sequentially comprises a first section and a second section along the length direction, the first section extends into the mounting groove, the second section is located outside the mounting groove, and the magnetic sleeve is arranged on the first section.

In a possible embodiment, the second section is provided with a positioning protrusion, and the positioning protrusion abuts against an end face of the magnetic sleeve mounting plate.

In a second aspect, the present application provides a magnetic attraction mechanism, comprising a support frame, a bar magnet frame assembly and a magnetic sleeve frame assembly as described above;

the magnetic sleeve frame component and the magnetic rod frame component are respectively movably arranged on the supporting frame, and the magnetic rod frame component can move to be sleeved with the magnetic sleeve frame component.

In one possible embodiment, the bar magnet assembly comprises a bar magnet mounting plate and a bar magnet assembly;

be equipped with the bar magnet installing zone on the bar magnet installing plate, the bar magnet installing zone is provided with a plurality of installation positions along first direction interval, and a plurality of bar magnet subassemblies are detachable respectively and install the installation position that corresponds in a plurality of installation positions, and a plurality of installation positions are configured to make a plurality of bar magnet subassemblies have two kind at least modes of arranging.

In a third aspect, the present application provides a sample extraction device, comprising a rack, an incubator, and the magnetic attraction mechanism described above;

the frame has the workstation, and incubator and magnetism inhale the mechanism and all install in the workstation.

The application provides a magnetism cover frame subassembly, magnetism inhale mechanism and sample and draw equipment, magnetism cover frame subassembly sets up a plurality of mounting grooves along the first direction interval in each magnetism cover installing zone at the magnetism cover mounting panel, pegs graft the magnetism cover subassembly in the mounting groove that corresponds. The magnetic sleeve component can be arranged in at least two ways through the plurality of mounting grooves, the magnetic sleeve component is flexible in configuration and high in space utilization rate, the magnetic sleeve component can be adapted to kits with at least two ways, a proper configuration way can be selected according to the requirement of sample extraction, the extraction flux of the sample extraction equipment is increased, and the sample extraction efficiency is improved.

Drawings

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required to be used in the description of the embodiments or the prior art are briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present application. For a person skilled in the art, without inventive effort, further figures can be obtained from these figures.

Fig. 1 is a schematic external view of a sample extraction device provided in an embodiment of the present application;

FIG. 2 is a schematic view of the sample extraction device of FIG. 1 in an open state;

FIG. 3 is a schematic diagram of the internal structure of the sample extraction device of FIG. 1;

FIG. 4a is a schematic view of a configuration of a kit provided in an embodiment of the present application;

FIG. 4b is a schematic view of another configuration of a kit provided in the examples of the present application;

FIG. 5a is a schematic structural diagram of a magnetic attraction mechanism according to an embodiment of the present disclosure;

FIG. 5b is a schematic structural diagram of a magnetic attraction mechanism according to another structural manner provided in the present application;

FIG. 6 is a schematic structural diagram of a magnetic bar mounting plate according to an embodiment of the present disclosure;

FIG. 7 is a schematic structural diagram of a magnetic rod assembly provided in an embodiment of the present application;

FIG. 8 is a schematic structural diagram of a magnetic sleeve mounting plate according to an embodiment of the present disclosure;

FIG. 9a is a partial structural view of a magnetic attraction mechanism provided in the embodiments of the present application;

FIG. 9b is a partial structural view of a magnetic attraction mechanism without a magnetic sleeve assembly according to an embodiment of the present disclosure;

FIG. 10 is a schematic structural diagram of a magnetic sleeve assembly provided in an embodiment of the present application;

fig. 11 is a schematic structural view of a closure according to an embodiment of the present application;

fig. 12a is a schematic structural diagram of an operation mode of an incubation module provided in an embodiment of the present application;

fig. 12b is a schematic structural diagram of another operation mode of the incubation module provided in this embodiment.

Detailed Description

In the process of analyzing biological samples such as human organ tissues, whole blood, plasma, serum, biological fluids and the like, the samples are usually extracted and purified first, and then the extracted and purified samples are detected and analyzed. Taking nucleic acid extraction as an example, firstly, cell lysis is performed on the collected sample, nucleic acid is separated and extracted, and then, the nucleic acid is purified, so that high-purity nucleic acid is obtained.

At present, the magnetic bead method which is convenient and efficient to operate is generally adopted in the market to extract nucleic acid, a kit containing a sample is placed on a workbench of nucleic acid extraction equipment, the nucleic acid extraction equipment adsorbs the magnetic bead at the bottom of a magnetic sleeve by utilizing the motion of the magnetic rod and the magnetic sleeve, the magnetic bead is transferred in reagents in different extraction holes of the kit, the reagents are rapidly stirred by utilizing the high-frequency up-and-down movement of the magnetic rod and the magnetic sleeve, the nucleic acid extraction processes such as cell lysis, nucleic acid adsorption, washing, elution and the like are realized, and finally high-purity nucleic acid is obtained.

However, in the related art, the configuration of the nucleic acid extraction device is single, and for samples (such as throat swab samples) which are in large demand and have a simple extraction process, the nucleic acid extraction device has a low utilization rate of the kit, which easily causes waste of consumables, increases the cost of nucleic acid extraction, and thus limits the extraction flux of the nucleic acid extraction device, and has low sample extraction efficiency.

In view of this, the embodiment of the present application provides a magnetic sleeve assembly, a magnetic attraction mechanism and a sample extraction device, in which the magnetic sleeve assembly is inserted into a corresponding mounting groove by disposing a plurality of mounting grooves at intervals along a first direction in each magnetic sleeve mounting area of a magnetic sleeve mounting plate. The magnetic sleeve component can be arranged in at least two ways through the plurality of mounting grooves, the magnetic sleeve component is flexible in configuration and high in space utilization rate, the magnetic sleeve component can be adapted to kits with at least two ways, a proper configuration way can be selected according to the requirement of sample extraction, the extraction flux of the sample extraction equipment is increased, and the sample extraction efficiency is improved.

In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

Fig. 1 is a schematic external view of a sample extraction device provided in an embodiment of the present application; fig. 2 is a schematic view of the sample extraction device of fig. 1 in an open state. Referring to fig. 1 and 2, the present embodiment provides a sample extraction device 1, where the sample extraction device 1 is used to extract particles/molecules required by a (biological) sample, for example, the sample extraction device 1 is used to extract nucleic acids in cells, or the sample extraction device 1 may also be used to extract proteins, enzymes, and other substances in cells, and the present embodiment is not limited thereto. The sample collection apparatus 1 of the present embodiment will be described below by taking nucleic acid collection as an example.

The sample extraction device 1 (hereinafter referred to as a device) of the present embodiment includes a chassis 100, and a rack 200, an incubator 300, a magnetic attraction mechanism 400, and other components disposed in the chassis 100, where the rack 200 is used as a main body support structure, and the operation components such as the incubator 300 and the magnetic attraction mechanism 400 are all mounted on the rack 200. Wherein, the rack 200 can be provided with a workbench 210, the incubator 300 and the magnetic attraction mechanism 400 can be both arranged on the workbench 210, when nucleic acid extraction is carried out, a kit containing a sample is arranged on the incubator 300, the incubator 300 heats an extraction reagent with temperature requirement in the kit, and the magnetic bead and the transfer nucleic acid are adsorbed by the movement of the magnetic attraction mechanism 400 so as to extract high-purity nucleic acid.

By installing the rack 200 in the chassis 100, a relatively closed environment is formed inside the chassis 100 during the extraction process, and for the extraction of pathogens of highly pathogenic and easily transmitted diseases appearing in recent years, samples are in the chassis 100, so that the risk of virus infection of detection personnel is not easily increased. Of course, when a sample containing no pathogens such as viruses and bacteria is taken, the sample may be exposed to the air, and in this case, the rack 200 may be exposed to the external environment, in other words, the cabinet 100 may not be provided.

As shown in fig. 1 and 2, the chassis 100 may include a box 110 and a hatch 120. The side of the casing 110 from which the operator introduces the reagent vessels into the casing 110, placed on the work table 210, is open, which is typically the operating side of the apparatus 1. The openable cover of the hatch door 120 is arranged on the opening of the box body 110, when the hatch door 120 is opened, the operation of taking and placing the reagent box can be carried out, and in the process of extraction, the hatch door 120 can be kept closed. Illustratively, the upper end of the door 120 may be rotatably coupled to the top of the chassis 100.

Referring to fig. 1, the outer surface of the box body 110 may be provided with a display screen 111 and an indicator light 112, and the display screen 111 and the indicator light 112 may be disposed at a side where the hatch 120 is located, for example, for an operator to operate and observe. The display screen 111 may be a touch screen, and can support a nucleic acid extraction operation in a touch manner; the indicator light 112 is used for indicating the working status of the device 1, the indicator light 112 may indicate the working status of the device 1 by displaying different colors, for example, the indicator light 112 is not turned on to indicate that the device 1 is turned off, idle or not running, the indicator light 112 is green to indicate that the extraction operation is in progress, and the indicator light 112 is red to indicate that the device 1 is failed.

In addition, the housing 110 may further include a power key 113, a speaker 114, a data interface 115, and the like, wherein the operator turns on or off the device 1 by pressing the power key 113, the speaker 114 is used to emit a warning sound or an alarm sound, and the data interface 115 (e.g., a USB interface) is used for data communication between the device 1 and a server or other devices 1. The box body 110 may further have a vent 116, where the vent 116 is formed by a plurality of vents arranged on a sidewall of the chassis 100, and the chassis 100 realizes convection of air inside and outside through the vent 116.

Referring to fig. 2, the housing 110 may be provided therein with an ultraviolet lamp 117, a fan 118, a scanner 119, and the like, and the ultraviolet lamp 117, the fan 118, and the scanner 119 may be mounted on an inner sidewall of the housing 110, for example. The ultraviolet lamp 117 is used for disinfecting and sterilizing the interior of the box body 110 after the extraction process is finished; the fan 118 is used for accelerating the air flow in the box body 110 and reducing aerosol pollution among samples of different batches; the scanner 119 is used to scan the barcode of the kit for entry of the extraction program.

Fig. 3 is a schematic view of the internal structure of the sample extraction device in fig. 1. Referring to fig. 3, the working platform 210 is, for example, a bottom plate installed at the bottom of the case 110, and the incubator 300 and the magnetic attraction mechanism 400 are installed on the bottom plate. In order to improve the efficiency of nucleic acid extraction, the working platform 210 may be provided with a plurality of mounting areas, and the reagent cartridges (not shown) are arranged in the mounting areas, one mounting area corresponding to each reagent cartridge, so that the apparatus 1 can simultaneously extract samples in a plurality of reagent cartridges. Wherein, the installation area can be set in a manner of array arrangement to improve the space utilization of the workbench 210.

For example, the mounting regions may be arranged on the worktable 210 in an array, and the X direction is a row direction and the Y direction is a column direction as shown in the figure, which shows that 6 mounting regions of 2 rows and 3 columns are arranged on the worktable 210, that is, the apparatus 1 can simultaneously extract samples in 6 reagent cartridges at a time. In other examples, the number of rows of the mounting areas arranged on the workbench 210 may also be 1 row, 3 rows, 4 rows or even more, and the number of columns of the mounting areas may also be 1 column, 2 columns, 4 columns, 5 columns or even more, depending on the volume size of the workbench 210, which is not particularly limited in this embodiment.

Corresponding to the plurality of installation areas on the worktable 210, the incubator 300 may include a plurality of incubation modules 310, each incubation module 310 is installed in each installation area in a one-to-one correspondence, the reagent kit is placed on the incubation module 310, and the reagent kit is heated by the incubation module 310. Wherein, incubation module 310 includes a plurality of incubation subassemblies 311, heats for the required extraction hole site of kit through incubation subassembly 311 to guarantee the required temperature requirement of extraction process.

Specifically, the workbench 210 may be provided with a limiting seat 211, the limiting seat 211 may be set to be a retaining wall structure, and an area surrounded by the retaining wall is an installation area. Incubation module 310 is correspondingly installed in the region that spacing seat 211 encloses, and the kit is placed in the region that spacing seat 211 encloses, carries out spacing fixed to the kit through spacing seat 211, and the kit is correspondingly arranged on incubation module 310.

With continued reference to fig. 3, the magnetic attachment mechanism 400 includes a support bracket 410, a magnet bar assembly 420, and a magnet sleeve assembly 430. The support frame 410 is movably mounted on the worktable 210, and for example, the worktable 210 may be mounted with a first motor 212, and the support frame 410 is driven by the first motor 212 to move along a first direction (X direction). Both the bar magnet assembly 420 and the magnetic sleeve assembly 430 are movably mounted on the support frame 410, the bar magnet assembly 420 may be located on a side of the magnetic sleeve assembly 430 facing away from the table 210, i.e., the bar magnet assembly 420 may be located above the magnetic sleeve assembly 430, and the bar magnet assembly 420 and the magnetic sleeve assembly 430 may be moved to be nested or separated with each other.

Wherein, bar magnet subassembly 420 includes bar magnet frame 421, second motor 423 and a plurality of bar magnet subassembly 422, and the bar magnet frame 421 is mobilizable to be installed in support frame 410, and bar magnet subassembly 422 is installed on bar magnet frame 421, and sets up perpendicularly in the one side of bar magnet frame 421 towards workstation 210, and second motor 423 for example can install on support frame 410, drives bar magnet frame 421 through second motor 423 vertical migration to drive bar magnet subassembly 422 vertical migration.

The magnetic sleeve assembly 430 comprises a magnetic sleeve frame 431, a third motor 433 and a plurality of magnetic sleeve assemblies 432, wherein the magnetic sleeve frame 431 is movably arranged on the support frame 410, the magnetic sleeve frame 431 is located between the magnetic rod frame 421 and the workbench 210, the magnetic sleeve assemblies 432 are detachably arranged on the magnetic sleeve frame 431 and vertically arranged on one side, facing the workbench 210, of the magnetic sleeve frame 431, each magnetic sleeve assembly 432 corresponds to each magnetic rod assembly 422, the third motor 433 can be arranged on the support frame 410 for example, and the magnetic sleeve frame 431 is driven to vertically move through the third motor 433 so as to drive the magnetic sleeve assemblies 432 to vertically move.

Specifically, the magnetic rod assembly 422 may include a plurality of magnetic rods 4221 arranged in sequence, the magnetic sleeve assembly 432 may include a plurality of magnetic sleeves 4321 arranged in sequence, and each magnetic rod 4221 may be inserted into each magnetic sleeve 4321 correspondingly. In this way, each magnetic rod assembly 422 and each magnetic sleeve assembly 432 can simultaneously realize the extraction operation of a plurality of samples, and the nucleic acid extraction efficiency can be improved.

When nucleic acid extraction is performed, the magnetic rod rack assembly 420 moves downwards to be sleeved with the magnetic sleeve rack assembly 430, each magnetic rod 4221 of the magnetic rod assembly 422 is inserted into each magnetic sleeve 4321 of the magnetic sleeve assembly 432, the first motor 212 drives the support frame 410 to move along the first direction (X direction), and the magnetic rod 4221 and the magnetic sleeve 4321 realize nucleic acid extraction operation together; after extraction is finished, the bar magnet assembly 420 moves upwards to be separated from the magnetic sleeve assembly 430, and the bar magnet assembly 422 and the magnetic sleeve assembly 432 are staggered up and down so as to be convenient for replacing the magnetic sleeve assembly 432.

In this embodiment, the magnetic attraction mechanism 400 and the incubator 300 are designed to have at least two structural modes for the magnetic attraction mechanism 400, and correspondingly, each incubation module 310 of the incubator 300 has at least two working modes for the magnetic attraction mechanism 400 and the incubator 300 to adapt to a kit having at least two configuration modes. Therefore, the configuration mode of the equipment 1 is more flexible, the extraction flux of the equipment 1 can be increased, and the extraction efficiency of the equipment 1 is improved; and, the configuration mode of the kit that matches with equipment 1 and use is also more nimble, and is higher to the utilization ratio of kit, can prevent to cause the consumptive material extravagant, reduces the sample and draws the cost.

In the magnetic attraction mechanism 400 with different structural modes, the magnetic rod assemblies 422 in the magnetic rod assembly 420 have different layout structures, and the magnetic sleeve assemblies 432 in the magnetic sleeve assembly 430 have different layout structures corresponding to the magnetic rod assemblies 422, and the magnetic sleeve assemblies 432 are matched with the layout structures of the magnetic rod assemblies 422; in the incubator 300 with different operation modes, the incubation component 311 in each incubation module 310 can heat the extraction holes at different positions of the kit. The layout structure of the magnetic rod assembly 422 (the magnetic sleeve assembly 432) and the working mode of the incubation module 310 are adapted to the configuration mode of the reagent kit, so that the magnetic rod assembly 422 (the magnetic sleeve assembly 432) with different layout structures and the incubation module 310 with different working modes can be adapted to the reagent kits with different configuration modes.

The following describes the nucleic acid extraction process, the magnetic attraction mechanism 400, and the incubation module 310 with reference to the layout structure of the kit.

FIG. 4a is a schematic view of a configuration of a kit provided in an embodiment of the present application; fig. 4b is a schematic view of another configuration of the kit provided in the embodiments of the present application. Referring to FIGS. 4a and 4b, many of the conventional nucleic acid extraction kits 2 on the market are 96-well plates, in which 96 extraction wells are arrayed, 8 lines (A-H) of extraction wells are provided, and 12 extraction wells are arrayed in each line. Corresponding to each step of nucleic acid extraction, the extraction well may include a lysis well 201, a washing well 202, an elution well 203, and a magnetic bead well 204, wherein the lysis well 201, the washing well 202, and the elution well 203 are filled with corresponding extraction reagents, and the magnetic bead well 204 is filled with magnetic beads.

When extracting nucleic acid, firstly putting a sample into the cracking hole 201 for cracking, releasing the nucleic acid from cells, then enabling the magnetic rod 4221 and the magnetic sleeve 4321 to enter the magnetic bead hole 204 to adsorb magnetic beads at the bottom of the magnetic sleeve 4321, enabling the magnetic rod 4221 and the magnetic sleeve 4321 to carry the magnetic beads to be sequentially transferred in the cracking hole 201, the washing hole 202 and the elution hole 203, adsorbing the nucleic acid in the cracking hole 201 on the surface of the magnetic beads, enabling the magnetic beads adsorbed with the nucleic acid to enter the washing hole 202, washing to remove impurities such as protein and polysaccharide except the nucleic acid, and then enabling the magnetic beads to enter the elution hole 203 to dissociate the nucleic acid adsorbed on the magnetic beads, and finally obtaining the high-purity and high-concentration nucleic acid.

During extraction, incubation module 310 heats the extraction reagents in lysis well 201 and elution well 203 to provide the temperature requirements for lysis and elution. Illustratively, in conjunction with fig. 3, the incubation component 311 is disposed below the lysis well 201 and the elution well 203 to heat the extraction reagent in the lysis well 201 and the elution well 203 to meet the extraction requirement.

Continuing to refer to fig. 4a and 4b, for example, for convenience of operation, the lysis well 201, the washing well 202, the elution well 203, and the magnetic bead well 204 required for a single sample may be arranged in sequence along the row direction of the reagent cartridge 2, and this embodiment defines all the extraction wells included for completing 1 sample extraction operation as 1 sample unit, that is, each sample unit includes the lysis well 201, the washing well 202, the elution well 203, and the magnetic bead well 204. The number of washing steps performed in the extraction process may be different, and the number of washing wells 202 included in each sample unit may be different, depending on the requirements of the sample, the quality of the extraction reagent, and the like.

For example, in fig. 4a, taking the number of washing times as 3 as an example, each sample unit includes 1 lysis well 201,3 washing wells 202, 1 elution well 203, and 1 magnetic bead well 204, for a 96-well plate, 12 extraction wells in each row correspond to 2 sample units, and 8 extraction wells in each row correspond to 16 sample units, that is, the 96-well plate can complete extraction of 16 samples at most; in fig. 4b, taking the number of washing times as 1 as an example, each sample unit includes 1 lysis well 201, 1 washing well 202, 1 elution well 203, and 1 magnetic bead well 204, for a 96-well plate, 12 extraction wells in each row correspond to 3 sample units, and 8 rows of extraction wells correspond to 24 sample units, that is, the 96-well plate can complete extraction of 24 samples at most.

As shown in fig. 3, the magnetic rod rack assembly 420 (magnetic sleeve rack assembly 430) may be provided with a plurality of magnetic rod assemblies 422 (magnetic sleeve assemblies 432) corresponding to each reagent cartridge 2, each magnetic rod assembly 422 (magnetic sleeve assembly 432) may be arranged at intervals along a first direction (X direction), the first direction is the row direction of the reagent cartridge 2, and each magnetic rod assembly 422 (magnetic sleeve assembly 432) is used to correspond to each sample unit one by one.

Moreover, for one magnetic rod assembly 422 (magnetic sleeve assembly 432) formed by sequentially arranging a plurality of magnetic rods 4221 (magnetic sleeves 4321), each magnetic rod 4221 (magnetic sleeve 4321) of each magnetic rod assembly 422 (magnetic sleeve assembly 432) may be sequentially arranged along the second direction (Y direction) so that the arrangement direction of each magnetic rod 4221 (magnetic sleeve 4321) of each magnetic rod assembly 422 (magnetic sleeve assembly 432) corresponds to the column direction of the kit 2, and each magnetic rod assembly 422 (magnetic sleeve assembly 432) may perform extraction operation on a plurality of samples at the same time. For example, each magnetic rod assembly 422 (magnetic sleeve assembly 432) may be provided with 8 magnetic rods 4221 (magnetic sleeves 4321) corresponding to the layout structure of a 96-well plate, so that one magnetic rod assembly 422 (magnetic sleeve assembly 432) can perform the extraction operation on 8 samples placed in each row of the reagent cartridge 2 at the same time.

FIG. 5a is a schematic structural diagram of a magnetic attraction mechanism according to an embodiment of the present disclosure; fig. 5b is a schematic structural diagram of a magnetic attraction mechanism according to another structural manner provided in the embodiment of the present application. Referring to fig. 5a and 5b, the number of the magnetic rod assemblies 422 (magnetic sleeve assemblies 432) and the distance between the magnetic rod assemblies 422 (magnetic sleeve assemblies 432) corresponding to each reagent cartridge 2 are different in the magnetic rod assembly 420 (magnetic sleeve assembly 430) having different structures corresponding to different arrangement of the reagent cartridges 2. Illustratively, the smaller the number of washing wells 202 included in a sample cell of the cartridge 2, the smaller the number of extraction wells included in each sample cell, the smaller the length range of the row direction of the cartridge 2 occupied by each sample cell, and the larger the number of sample cells arranged per row of the cartridge 2.

Referring to fig. 4a and 5a, taking the configuration of the reagent cartridge 2 with 2 sample units arranged in each row as an example, the magnetic rod assembly 420 (magnetic sleeve assembly 430) is configured in the following manner: corresponding to each reagent cartridge 2, 2 magnetic rod assemblies 422 (magnetic sleeve assemblies 432) are arranged at intervals along a first direction (X direction, corresponding to the row direction of the reagent cartridge 2), and the distance between each magnetic rod assembly 422 (magnetic sleeve assembly 432) is the width of 5 extraction holes; referring to fig. 4b and 5b, taking the configuration of the reagent cartridge 2 with 3 sample units arranged in each row as an example, the magnetic rod assembly 420 (magnetic sleeve assembly 430) is configured in the following manner: corresponding to each reagent kit 2, 3 magnetic rod assemblies 422 (magnetic sleeve assemblies 432) are arranged at intervals in the first direction on the magnetic rod assembly 420 (magnetic sleeve assembly 430), and the second distance between the magnetic rod assemblies 422 (magnetic sleeve assemblies 432) is the width of 3 extraction holes.

Referring to fig. 5a or 5b, the bar magnet holder 421 of the bar magnet holder assembly 420 may include a fixing holder 4211 and a bar magnet mounting plate 4212. The fixing bracket 4211 is movably mounted on the supporting frame 410, for example, a linear guide 411 extending in a vertical direction may be disposed on the supporting frame 410, and the fixing bracket 4211 moves along the linear guide 411 and is driven by the second motor 423 to move. The bar magnet mounting plate 4212 is connected to the fixing frame 4211, the bar magnet mounting plate 4212 is horizontally arranged, for example, the bar magnet assembly 422 is mounted on one side surface of the bar magnet mounting plate 4212 facing the workbench 210, and each bar magnet 4221 on the bar magnet assembly 422 can keep a natural sagging state.

Fig. 6 is a schematic structural diagram of a magnetic rod mounting plate according to an embodiment of the present application. Referring to fig. 5a, 5b and 6, the bar magnet mounting plates 4212 are provided with bar magnet mounting areas 4212a, and one bar magnet mounting area 4212a corresponds to one mounting area on the work table 210, that is, one bar magnet mounting area 4212a corresponds to one reagent cartridge 2. For the case that a plurality of mounting areas are arranged on the workbench 210, a plurality of bar magnet mounting areas 4212a may be arranged on the bar magnet mounting plate 4212, and the bar magnet mounting areas 4212a may also be arranged in an array matching the array arrangement of the mounting areas.

The magnetic rod mounting area 4212a is provided with a plurality of mounting positions 42121 at intervals along the first direction (X direction), and the magnetic rod assembly 422 is detachably mounted on the mounting positions 42121. The plurality of mounting positions 42121 in the magnetic rod mounting area 4212a can enable the magnetic rod assembly 422 to have at least two arrangements, and can be adapted to the reagent kit 2 having at least two arrangements. For example, the number of the mounting positions 42121 in the bar magnet mounting area 4212a and the spacing between the mounting positions 42121 may be designed, so that all the mounting positions 42121 in the bar magnet mounting area 4212a may realize at least two arrangements of the bar magnet assemblies 422, and the bar magnet assembly 420 has at least two architectures.

Taking the example shown in fig. 6 as an example, 4 mounting positions 42121 may be arranged at intervals in the first direction (X direction) in each magnet bar mounting area 4212a, and in the case that 2 magnet bar assemblies 422 need to be mounted in each magnet bar mounting area 4212a, as shown in fig. 5a and 6, 2 magnet bar assemblies 422 may be mounted in 1 and 3 of the 4 mounting positions 42121, respectively; in the case that 3 bar magnet assemblies 422 are required to be installed in each bar magnet installation area 4212a, as shown in fig. 5b and 6, 3 bar magnet assemblies 422 may be installed in 1, 2 and 4 of the 4 installation sites 42121, respectively.

In addition, in order to reduce the weight of the bar magnet mounting plate 4212 and improve the flexibility and stability of the movement of the bar magnet mounting plate 4212, the bar magnet mounting plate 4212 may be further provided with lightening holes a. For example, the lightening holes a may be formed in a region between adjacent bar magnet mounting regions 4212a, a region in each bar magnet mounting region 4212a where a space between adjacent mounting positions 42121 is large, or the like.

Fig. 7 is a schematic structural diagram of a magnetic rod assembly according to an embodiment of the present application. Referring to fig. 6 and 7, as an embodiment, the magnetic rod assemblies 422 may be detachably mounted to the corresponding mounting locations 42121 by means of screw connection. For example, each of the mounting positions 42121 on the bar magnet mounting plate 4212 may be provided with a first mounting hole 42121a, and correspondingly, the bar magnet assembly 422 may be provided with a second mounting hole 42221, the second mounting hole 42221 corresponds to the first mounting hole 42121a, and the bar magnet assembly 422 is mounted on the bar magnet mounting plate 4212 by inserting locking members such as screws and bolts into the first mounting hole 42121a and the second mounting hole 42221.

In order to assemble the plurality of magnetic rods 4221 into the integrated magnetic rod assembly 422, the magnetic rod assembly 422 may be provided with a fixing block 4222 as a mounting base for the magnetic rods 4221, each magnetic rod 4221 is mounted on the fixing block 4222, the fixing block 4222 is connected to the magnetic rod mounting plate 4212, that is, the second mounting hole 42221 may be opened in the fixing block 4222. For example, in order to accurately position the magnetic rod assembly 422, a positioning column 42222 may be further disposed on the fixed block 4222, the positioning column 42222 extends out of a side surface of the fixed block 4222 facing the magnetic rod mounting plate 4212, a positioning hole 42121b may be formed in a corresponding portion of the mounting position 42121 of the magnetic rod mounting plate 4212, and when the magnetic rod assembly 422 is mounted, the positioning column 42222 on the magnetic rod assembly 422 extends into the positioning hole 42121b of the magnetic rod mounting plate 4212, so as to position the magnetic rod assembly 422.

In addition, in order to protect the magnetic rod 4221, the magnetic rod assembly 422 may further include a sleeve 4223, the sleeve 4223 is sleeved outside the magnetic rod 4221, and the sleeve 4223 should expose the bottom of the magnetic rod 4221 to ensure that the magnetic rod 4221 can smoothly and stably adsorb the magnetic beads. The sleeve 4223 is shielded from the outside of the magnetic rod 4221, so that the magnetic rod 4221 can be protected from being damaged and the magnetic rod 4221 can be prevented from being influenced by foreign matters such as dust. For example, the magnetic rod 4221 may be fixedly connected with the sleeve 4223 by means of bonding or the like, and the magnetic rod 4221 is fixed by the connection between the sleeve 4223 and the fixing block 4222. The sleeve 4223 may be made of a metal material, and for example, the sleeve 4223 is made of a material such as stainless steel, aluminum alloy, or titanium alloy.

With continued reference to fig. 5a or 5b, the magnet housing shelf 431 of the magnet housing assembly 430 may include a connection shelf 4311 and a magnet housing mounting plate 4312. The connection frame 4311 is movably mounted on the supporting frame 410, for example, the connection frame 4311 can move along the linear guide 411 arranged on the supporting frame 410, and the connection frame 4311 is driven by the third motor 433 to move. The magnetic shield mounting plate 4312 is connected to the connection frame 4311, the magnetic shield mounting plate 4312 is arranged horizontally, for example, and the magnetic shield assembly 432 is detachably mounted on a side surface of the magnetic shield mounting plate 4312 facing the worktable 210, so that each magnetic shield 4321 of the magnetic shield assembly 432 can keep a natural drooping state.

In some embodiments, in the case that the magnetic jacket assemblies 432 on the magnetic jacket frame 431 are arranged in a plurality of rows along the second direction (Y direction), in this embodiment, the magnetic jacket frame 431 may be a separable structure, for example, the magnetic jacket frame 431 may include a first magnetic jacket frame 431a and a second magnetic jacket frame 431b, the first magnetic jacket frame 431a and the second magnetic jacket frame 431b are arranged in the front and back direction along the second direction, and at least one row of magnetic jacket assemblies 432 are respectively mounted on the first magnetic jacket frame 431a and the second magnetic jacket frame 431b along the second direction, so that the mounting and dismounting of the magnetic jacket assemblies 432 are facilitated, the operation of the apparatus 1 is simplified, and the sample extraction efficiency is improved.

During the extraction of nucleic acid, the first magnetic holder 431a and the second magnetic holder 431b may be at the same horizontal position, so as to implement the extraction operation of the magnetic holder 432 on the first magnetic holder 431a and the magnetic holder 432 on the second magnetic holder 431 b; after extraction is finished, the first magnetic sleeve frame 431a and the second magnetic sleeve frame 431b can be controlled to move to be separated up and down, so that the magnetic sleeve assembly 432 on the first magnetic sleeve frame 431a and the magnetic sleeve assembly 432 on the second magnetic sleeve frame 431b are staggered up and down, and the magnetic sleeve assembly 432 can be conveniently taken out and installed.

In the case where the magnetic jacket frame 431 includes the first magnetic jacket frame 431a and the second magnetic jacket frame 431b, both the first magnetic jacket frame 431a and the second magnetic jacket frame 431b may be composed of the connection frame 4311 and the magnetic jacket installation plate 4312. The widths of the magnetic shield mounting plates 4312 of the first and second magnetic shield shelves 431a and 431b in the second direction (Y direction) may be the same or different depending on the number (line number) of the magnetic shield assemblies 432 to which the first and second magnetic shield shelves 431a and 431b are respectively mounted.

Moreover, the magnetic sleeve mounting plate 4312 of the first magnetic sleeve holder 431a and the mounting plate of the second magnetic sleeve holder 431b are arranged in front and back in the second direction, taking as an example that the magnetic sleeve mounting plate 4312 of the first magnetic sleeve holder 431a is located on the side, away from the support frame 410, of the magnetic sleeve mounting plate 4312 of the second magnetic sleeve holder 431b, the extension length of the connecting frame 4311 of the first magnetic sleeve holder 431a in the second direction should be greater than the extension length of the connecting frame 4311 of the second magnetic sleeve holder 431b in the second direction, and the connecting frame 4311 of the first magnetic sleeve holder 431a is connected with the magnetic sleeve mounting plate 4312 of the first magnetic sleeve holder 431a across the magnetic sleeve mounting plate 4312 of the second magnetic sleeve holder 431 b.

As for the driving manner of the magnetic jacket frame assembly 430, in some embodiments, only one third motor 433 may be provided, and the third motor 433 may drive the first magnetic jacket frame 431a (the second magnetic jacket frame 431 b) to move, and the first magnetic jacket frame 431a (the second magnetic jacket frame 431 b) drives the second magnetic jacket frame 431b (the first magnetic jacket frame 431 a) to move; in other embodiments, two third motors 433 may be provided, and the two third motors 433 respectively drive the first and second magnetic mount 431a and 431b to move.

FIG. 8 is a schematic structural diagram of a magnetic sleeve mounting plate according to an embodiment of the present disclosure; FIG. 9a is a partial structural view of a magnetic attraction mechanism provided in the embodiments of the present application; fig. 9b is a partial structural view of the magnetic attraction mechanism without the magnetic sleeve assembly according to the embodiment of the present application.

Referring to fig. 8 and 9a, similar to the magnetic rod mounting plate 4212, the magnetic sleeve mounting plate 4312 is provided with magnetic sleeve mounting areas 4312a, one magnetic sleeve mounting area 4312a corresponds to one mounting area on the work table 210, that is, one magnetic sleeve mounting area 4312a corresponds to one reagent kit 2, and the magnetic sleeve mounting areas 4312a correspond to the magnetic rod mounting areas 4212a one to one. For the case that a plurality of mounting areas are arranged on the worktable 210, a plurality of magnetic sleeve mounting areas 4312a may be arranged on the magnetic sleeve mounting plate 4312, and the magnetic sleeve mounting areas 4312a may also be arranged in an array matching the array arrangement of the mounting areas.

As shown in fig. 9a and 9b, in order to facilitate the installation and removal of the magnetic sleeve assembly 432, in this embodiment, the magnetic sleeve assembly 432 may be installed on the magnetic sleeve installation plate 4312 in an inserting manner. Specifically, a plurality of mounting grooves 43121 are disposed in each magnetic sleeve mounting region 4312a of the magnetic sleeve mounting plate 4312 at intervals along a first direction (X direction), the mounting grooves 43121 may extend along a second direction (Y direction), and the magnetic rod assemblies 422 are inserted into the corresponding mounting grooves 43121.

The plurality of mounting slots 43121 in the magnetic sleeve mounting region 4312a can enable the magnetic sleeve assembly 432 to have at least two arrangements, and can be adapted to the reagent cartridges 2 having at least two arrangements. Similar to the bar magnet mounting plate 4212, the number of the mounting slots 43121 in the magnetic sleeve mounting region 4312a and the distance between the mounting slots 43121 can be designed, so that all the mounting slots 43121 in the magnetic sleeve mounting region 4312a can realize at least two arrangement modes of the magnetic sleeve component 432, and the magnetic sleeve component 430 has at least two framework modes.

Taking fig. 9a or 9b as an example, 4 mounting slots 43121 may be disposed at intervals along the first direction (X direction) in each magnetic sleeve mounting region 4312 a. In the case that 2 magnetic sleeve assemblies 432 need to be installed in each magnetic sleeve installation region 4312a, as shown in fig. 8 and 5a, the 2 magnetic sleeve assemblies 432 can be respectively inserted into the installation grooves 43121 labeled as 1 and 3 in the drawing; in the case that 3 magnetic sleeve assemblies 432 are required to be installed in each magnetic sleeve installation region 4312a, as shown in fig. 8 and 5b, the 3 magnetic sleeve assemblies 432 can be respectively inserted into the installation grooves 43121 labeled as 1, 2 and 4 in the figure.

In addition, similar to the magnetic bar mounting plate 4212, the magnetic sleeve mounting plate 4312 may be provided with lightening holes a in order to reduce the weight of the magnetic sleeve mounting plate 4312. For example, the lightening holes a may be formed in a region between adjacent magnetic sleeve mounting regions 4312a, a region in each magnetic sleeve mounting region 4312a where a space between adjacent mounting grooves 43121 is large, or the like.

Referring to fig. 9b, for the mounting groove 43121 extending along the second direction (Y direction), one end of the mounting groove 43121 may be communicated to the end surface of the magnetic shield mounting plate 4312, the end of the mounting groove 43121 is defined as an insertion end thereof in this embodiment, and the magnetic shield assembly 432 may be inserted into the mounting groove 43121 from the insertion end of the mounting groove 43121. For example, to facilitate the mounting and dismounting of the magnetic sleeve assembly 432, the insertion end of the mounting slot 43121 may be disposed on the end surface of the magnetic sleeve mounting plate 4312 facing away from the supporting frame 410.

As for the positioning of the magnetic sleeve component 432 in the mounting groove 43121, in some examples, the mounting groove 43121 may be configured to have only the insertion end communicated to the end surface of the magnetic sleeve mounting plate 4312, while the other end of the mounting groove 43121 is a closed end, and the magnetic sleeve component 432 may abut against the closed end of the mounting groove 43121 to position the magnetic sleeve component 432; in other examples, it is also not limited whether the other end of the mounting groove 43121 is communicated to the other end surface of the magnetic sleeve mounting plate 4312, and the magnetic sleeve assembly 432 can be positioned by providing a positioning structure on the magnetic sleeve assembly 432 and by matching the positioning structure on the magnetic sleeve assembly 432 with the mounting groove 43121.

Fig. 10 is a schematic structural diagram of a magnetic sleeve assembly according to an embodiment of the present application. Referring to fig. 10, in order to assemble the plurality of magnetic housings 4321 into the integrated magnetic housing assembly 432, the magnetic housing assembly 432 may be provided with a fixing plate 4322 as a mounting base for the magnetic housings 4321, each magnetic housing 4321 is mounted on the fixing plate 4322, and the fixing plate 4322 is inserted into the mounting groove 43121. For example, for the magnetic sleeve assembly 432 that is not usually reused, the magnetic sleeve assembly 432 may be designed as a unitary structure, i.e. the magnetic sleeve 4321 and the fixing plate 4322 are integrally formed, thereby facilitating the production and manufacture of the magnetic sleeve assembly 432 and also facilitating the replacement of the magnetic sleeve assembly 432.

The fixing plate 4322 may include a first section 43221 and a second section 43222 along the length direction of the fixing plate 4322, and the magnetic sleeve 4321 may be disposed on the first section 43221 of the fixing plate 4322. Referring to fig. 9a and 10, when the magnetic sleeve assembly 432 is mounted on the magnetic sleeve mounting plate 4312, the first section 43221 of the fixing plate 4322 extends into the mounting groove 43121 to ensure that the magnetic sleeve assembly 432 and the magnetic rod assembly 422 are aligned accurately, the second section 43222 of the fixing plate 4322 is exposed out of the mounting groove 43121, the second section 43222 of the fixing plate 4322 provides a holding space for an operator, and the operator can mount and dismount the magnetic sleeve assembly 432 by holding the second section 43222 of the fixing plate 4322.

For example, the second section 43222 of the fixing plate 4322 may be provided with a positioning protrusion 43222a, and the positioning protrusion 43222a may serve as a positioning structure on the magnetic sleeve assembly 432, which may protrude from a side surface of the fixing plate 4322 facing away from the magnetic sleeve 4321. When the magnetic sleeve assembly 432 is mounted on the magnetic sleeve mounting plate 4312, the fixing plate 4322 is inserted into the mounting groove 43121 from the insertion end of the mounting groove 43121, the first section 43221 of the fixing plate 4322 is located in the mounting groove 43121, and the positioning protrusion 43222a of the second section 43222 of the fixing plate 4322 abuts against the end surface of the magnetic sleeve mounting plate 4312, so as to position the magnetic sleeve assembly 432.

Referring to fig. 9b and 10, when designing the magnetic sleeve assembly 432, the width of the fixing plate 4322 is generally greater than the outer diameter of the magnetic sleeve 4321 to ensure the stability of the connection between the magnetic sleeve 4321 and the fixing plate 4322 and ensure the overall strength of the magnetic sleeve assembly 432, so that the mounting groove 43121 adapted to the fixing plate 4322 should have a sufficient width to avoid affecting the strength of the magnetic sleeve mounting plate 4312, in some embodiments, the mounting groove 43121 may only occupy a part of the thickness space of the magnetic sleeve mounting plate 4312, and in this case, the mounting groove 43121 is disposed on the side of the magnetic sleeve mounting plate 4312 facing the worktable 210, in other words, the mounting groove 43121 is disposed on the side of the bottom surface of the magnetic sleeve mounting plate 4312.

In order to fit the magnetic sleeve module 432 to the magnetic rod module 422 located above the magnetic sleeve module 432, a communication groove 43122 may be formed in the magnetic sleeve mounting plate 4312, and a plurality of communication grooves 43122 may be provided at intervals in the first direction (X direction) in each magnetic sleeve mounting region 4312a corresponding to the mounting groove 43121, and the communication grooves 43122 may extend in the second direction (Y direction). The communication grooves 43122 correspond to the mounting grooves 43121 one by one, and in the thickness direction of the magnetic sleeve mounting plate 4312, the communication grooves 43122 occupy the remaining space except for the mounting groove 43121, that is, two sides of the communication grooves 43122 in the depth direction are respectively communicated with the top surface of the magnetic sleeve mounting plate 4312 and the mounting groove 43121, and the communication between the mounting groove 43121 and the top surface of the magnetic sleeve mounting plate 4312 is realized through the communication grooves 43122.

On the basis that the communication groove 43122 can ensure that the magnetic rod 4221 is smoothly inserted into the magnetic sleeve 4321, that is, on the basis that the width of the communication groove 43122 is larger than the outer diameter of the magnetic rod 4221, the width of the communication groove 43122 may be smaller than the width of the mounting groove 43121, so as to improve the strength of the magnetic sleeve mounting plate 4312. In some examples, the communication groove 43122 may be configured to have two closed ends, that is, a distance is formed between two ends of the communication groove 43122 and two ends of the magnetic sleeve mounting plate 4312, so as to reduce the volume of the communication groove 43122, ensure the strength of the magnetic sleeve mounting plate 4312, and facilitate the alignment and the sleeve joint of the magnetic rod assembly 422 and the magnetic sleeve assembly 432.

With continued reference to fig. 9a or 9b, in some embodiments, the magnetic sleeve assembly 430 may further include a blocking piece 434, the blocking piece 434 is configured to be inserted into an insertion end of the mounting groove 43121, and a position of the mounting groove 43121 where the magnetic sleeve assembly 432 needs to be mounted may be predetermined by using the blocking piece 434, so as to prevent an operator from inserting the magnetic sleeve assembly 432 into a wrong mounting groove 43121, ensure accuracy of mounting the magnetic sleeve assembly 432, reduce a probability of a wrong mounting of the magnetic sleeve assembly 432, and also help to improve mounting efficiency of the magnetic sleeve assembly 432.

Referring to fig. 8 and 5a, when 2 magnetic sleeve assemblies 432 are required to be installed in each magnetic sleeve installation area 4312a, and the 2 magnetic sleeve assemblies 432 are required to be respectively inserted into the installation grooves 43121 labeled 1 and 3 in the drawing, a plugging member 434 may be inserted into the installation groove 43121 labeled 2 and 4 in advance to plug the insertion ends of the installation grooves 43121 labeled 2 and 4; referring to fig. 8 and 5b, when 3 magnetic sleeve assemblies 432 are required to be installed in each magnetic sleeve installation area 4312a, and the 3 magnetic sleeve assemblies 432 are respectively inserted into the installation grooves 43121 labeled 1, 2 and 4 in the drawing, a plugging member 434 may be inserted into the installation groove 43121 labeled 3 in advance to plug the insertion end of the installation groove 43121 labeled 3.

Fig. 11 is a schematic structural view of a closure according to an embodiment of the present application. Referring to fig. 11, as an embodiment, the blocking piece 434 may include a blocking portion 4341 and a holding portion 4342, the blocking portion 4341 is used for blocking the mounting groove 43121, and the holding portion 4342 is used for being held by an operator. During operation, an operator holds the holding portion 4342 of the plugging member 434, inserts the plugging portion 4341 of the plugging member 434 into the insertion end of the mounting groove 43121, and the holding portion 4342 extends out of the mounting groove 43121, so that the plugging member 434 can be conveniently taken and mounted.

For example, the holding portion 4342 and the blocking portion 4341 may be arranged at a certain inclination angle, for example, an included angle between the holding portion 4342 and the blocking portion 4341 may be 90 °, so that the blocking piece 434 may be limited by the joint portion between the blocking portion 4341 and the holding portion 4342, which can ensure that the blocking piece 434 can be stably blocked at the insertion end of the mounting groove 43121, and meanwhile, the blocking piece 434 may be prevented from being inserted too deeply and being unable to be extracted.

Fig. 12a is a schematic structural diagram of an operation mode of an incubation module provided in an embodiment of the present application; fig. 12b is a schematic structural diagram of another operation mode of the incubation module provided in this embodiment. Referring to fig. 12a and 12b, as for the setting mode of the incubation module 310, in order to meet the requirement that the incubation module 310 can be adapted to reagent kits 2 with different configuration modes, in this embodiment, the incubation module 310 may be provided with a mounting seat 312, each incubation component 311 may be detachably mounted to the mounting seat 312, and the incubation module 310 is adapted to reagent kits 2 with different configuration modes by changing the combination mode of the incubation components 311.

Specifically, the operation mode of the incubation module 310 can be changed by controlling the number and the position of the incubation assemblies 311 installed in the incubation module 310. With reference to fig. 4a and 4b, continuing to take a 96-well plate as an example, for the case where 12 extraction holes are arranged in each row of the 96-well plate, fig. 4a shows that 1, 7 holes in each row are lysis holes 201,5, 11 holes are elution holes 203, fig. 4b shows that 1,5, 9 holes in each row are lysis holes 201,3, 7, 11 holes are elution holes 203, and for this, in conjunction with fig. 12a and 12b, in this embodiment, 6 incubation assemblies 311 may be arranged in the mounting seat 312, each incubation assembly 311 corresponds to the extraction holes in the 1 st, 3 rd, 5 th, 7 th, 9 th, 11 th rows, respectively, and all the incubation assemblies 311 of each incubation module 310 may satisfy the kit 2 in these two configurations.

As an embodiment, different numbers of incubation assemblies 311 can be installed in the mounting seat 312, so that the incubation module 310 can work in different modes, thereby meeting the requirements of the reagent kit 2 with different configurations. For example, as shown in fig. 4a and 12a, the reagent cartridge 2 is configured in such a manner that the extraction holes in the 1 st and 7 th rows are lysis holes 201, and the extraction holes in the 5 th and 11 th rows are elution holes 203, and at this time, 4 incubation assemblies 311 can be loaded into the mounting seat 312, and each incubation assembly 311 is correspondingly disposed below the extraction holes in the 1 st, 5 th, 7 th and 11 th rows; referring to fig. 4b and 12b, the reagent cartridge 2 is configured such that the extraction wells in columns 1,5, and 9 are lysis wells 201, and the extraction wells in columns 3, 7, and 11 are elution wells 203, and 6 incubation assemblies 311 can be loaded into the mounting seat 312, and each incubation assembly 311 is correspondingly disposed under the extraction wells in columns 1,3, 5, 7, 9, and 11, respectively.

As another embodiment, in order to avoid repeatedly disassembling and assembling the incubation module 311, the incubation module 310 may also be in a state where the incubation module 311 is fully loaded all the time, and the incubation module 310 may be switched between different working modes by controlling the working state of each incubation module 311. Exemplarily, referring to fig. 12b, taking the configuration of the reagent kit 2 shown in fig. 4a as an example, the 4 incubation assemblies 311 of 1 st, 3 rd, 4 th and 6 th in the incubation module 310 may be controlled to operate, and the 2 incubation assemblies 311 of 2 nd and 5 th do not operate; taking the configuration of the reagent kit 2 shown in fig. 4b as an example, all of the 6 incubation assemblies 311 in the incubation module 310 can be controlled to be in the working state.

In the description of the present application, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on those shown in the drawings, merely for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be considered limiting of the present application.

Finally, it should be noted that: the above embodiments are only used for illustrating the technical solutions of the present application, and not for limiting the same; although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present application.

Claims (12)

1. A magnetic carriage assembly, comprising: the magnetic sleeve mounting plate comprises a plurality of magnetic sleeve assemblies;

the magnetic sleeve mounting plate is provided with a magnetic sleeve mounting area, the magnetic sleeve mounting area is provided with a plurality of mounting grooves at intervals along a first direction, any one of the mounting grooves extends along a second direction, the magnetic sleeve assemblies are respectively inserted into corresponding mounting grooves in the mounting grooves, and the mounting grooves are configured to enable the magnetic sleeve assemblies to have at least two arrangement modes.

2. The magnet holder assembly of claim 1 wherein one end of the mounting slot is an insertion end that communicates to an end face of the magnet holder mounting plate.

3. The magnetic carriage assembly of claim 2 further comprising a closure member removably insertable into the insertion end of said mounting slot.

4. The magnetic sleeve assembly of claim 3 wherein said blocking piece includes a blocking portion inserted into said mounting slot and a holding portion extending out of said mounting slot.

5. The magnet holder assembly of any of claims 1-4 wherein the magnet holder mounting area is further provided with a plurality of communication grooves spaced along the first direction, any one of the plurality of communication grooves extending along the second direction; the communicating groove corresponds to the mounting grooves one by one, and two sides of the communicating groove in the depth direction are respectively communicated with the top surface of the magnetic sleeve mounting plate and the mounting grooves.

6. The magnetic carriage assembly of claim 5, wherein the width of the communication slot is less than the width of the mounting slot.

7. The magnetic sleeve assembly as claimed in any one of claims 1 to 4, wherein the magnetic sleeve assembly comprises a fixing plate and a plurality of magnetic sleeves, the fixing plate is detachably inserted into the mounting groove, and the plurality of magnetic sleeves are connected to the fixing plate and are sequentially arranged along the second direction.

8. The magnetic sleeve frame assembly as claimed in claim 7, wherein the fixing plate includes a first section and a second section in sequence along the length direction, the first section extends into the mounting groove, the second section is located outside the mounting groove, and the magnetic sleeve is disposed on the first section.

9. The holder assembly of claim 8 wherein the second section has a locating protrusion, the locating protrusion abutting against an end surface of the holder mounting plate.

10. A magnetic attraction mechanism, comprising a support frame, a bar magnet assembly and a magnetic sleeve assembly as claimed in any one of claims 1 to 9;

the magnetic sleeve frame assembly and the magnetic rod frame assembly are respectively movably arranged on the supporting frame, and the magnetic rod frame assembly can move to be sleeved with the magnetic sleeve frame assembly.

11. The mechanism of claim 10, wherein the bar magnet assembly comprises a bar magnet mounting plate and a bar magnet assembly;

be equipped with the bar magnet installing zone on the bar magnet installing plate, the bar magnet installing zone is provided with a plurality of installation positions along first direction interval, a plurality of bar magnet subassemblies respectively detachable install in corresponding installation position in a plurality of installation positions, a plurality of installation positions are configured to the messenger a plurality of bar magnet subassemblies have two kind at least modes of arranging.

12. A sample extraction device comprising a housing, an incubator, and a magnetic attachment mechanism as claimed in claim 10 or 11;

the frame has the workstation, hatch the ware with the mechanism is inhaled to magnetism all installs in the workstation.

Images