CN115746997A - Sample extraction device - Google Patents

Abstract

The application provides a sample extraction device, and the sample extraction device includes: a frame having a table; the incubator is arranged on the workbench and used for heating the kit loaded on the incubator; the magnetic attraction mechanism comprises a support frame and a magnetic rod frame assembly, the support frame is mounted on the workbench and moves along a first direction, the magnetic rod frame assembly is movably mounted on the support frame, and the magnetic rod frame assembly has at least two framework modes. The sample extraction equipment is flexible in configuration mode, sample extraction flux can be increased, extraction efficiency is high, and consumable utilization rate is high.

Description

Sample extraction device

Technical Field

The invention relates to the technical field of nucleic acid extraction, in particular to sample extraction equipment.

Background

Nucleic acid extraction is one of the most basic experiments in molecular experiments, and is the basis of almost all experiments, and nucleic acid can be successfully performed no matter subsequent cloning, polymerase Chain Reaction (PCR), quantitative real-time Polymerase Chain Reaction (Q-PCR), library construction and sequencing, and the like.

At present, the mainstream nucleic acid extraction equipment in the market mainly uses a magnetic bead method to purify nucleic acid, is matched with a kit for sample extraction, and finally obtains a purified target nucleic acid substance by assembling the kit containing the sample on the nucleic acid extraction equipment and carrying out steps of cracking, combining, washing, eluting and the like.

However, the existing nucleic acid extraction equipment and the reagent kit are single in configuration mode, and have low extraction efficiency and low consumable utilization rate for samples with large demand and simple extraction process.

Disclosure of Invention

In order to solve at least one problem mentioned in the background art, the application provides a sample extraction device, and the configuration mode of sample extraction device is nimble, can increase sample extraction flux, and extraction efficiency is high, consumptive material high-usage.

The application provides a sample extraction device, includes:

a frame having a table;

the incubator is arranged on the workbench and used for heating the kit carried on the incubator;

the magnetic attraction mechanism comprises a support frame and a magnetic rod frame assembly, the support frame is mounted on the workbench and moves along a first direction, the magnetic rod frame assembly is movably mounted on the support frame, and the magnetic rod frame assembly has at least two framework modes.

In one possible embodiment, the magnetic rod rack assembly comprises a magnetic rod rack and a plurality of magnetic rod assemblies, wherein the magnetic rod rack is movably arranged on the supporting frame, and the magnetic rod assemblies are vertically arranged on one side of the magnetic rod rack, which faces the reagent box;

in the bar magnet frame assemblies with different frame modes, the layout frame of the bar magnet assemblies on the bar magnet frame is different.

In one possible embodiment, the magnetic attraction mechanism comprises at least two sets of bar magnet frame assemblies, and the arrangement of the bar magnet assemblies on the bar magnet frame assemblies of different bar magnet frame assemblies is different.

In a possible implementation mode, the magnetic attraction structure comprises a magnetic rod frame assembly, wherein the magnetic rod frame is provided with a plurality of installation positions, and the magnetic rod assembly is detachably arranged on the installation positions;

the magnetic rod assembly is different in layout structure under different structure modes.

In one possible embodiment, the bar magnet frame assembly has a first configuration and a second configuration;

the magnetic rod rack assembly of the first framework mode is provided with M columns of magnetic rod assemblies which are arranged at intervals along the first direction corresponding to each reagent kit, and the magnetic rod rack assembly of the second framework mode is provided with N columns of magnetic rod assemblies which are arranged at intervals along the first direction corresponding to each reagent kit;

wherein M and N are positive integers, and M is less than N.

In one possible embodiment, the incubator comprises a plurality of incubation modules, each of which can carry one kit, and the incubation modules have at least two operation modes.

In one possible embodiment, the incubation module comprises a plurality of detachably arranged incubation assemblies, and the incubation assemblies are sequentially arranged along a first direction;

under different working modes, the working states of the incubation assemblies in the incubation module are different, or the number of the incubation assemblies loaded in the incubation module is different.

In one possible embodiment, the magnetic attraction structure further comprises a magnet holder assembly movably mounted to the support frame and located between the incubator and the magnet bar holder assembly.

In a possible embodiment, the magnetic sleeve assembly comprises a magnetic sleeve frame and a plurality of magnetic sleeve assemblies, the magnetic sleeve frame is movably arranged on the support frame, and the magnetic sleeve assemblies are vertically arranged on one side of the magnetic sleeve frame facing the kit;

the magnetic sleeve assemblies correspond to the magnetic rod assemblies one to one, and the magnetic rod assemblies can move to be inserted into the magnetic sleeve assemblies.

In one possible embodiment, the magnetic sleeve frame comprises a first magnetic sleeve frame and a second magnetic sleeve frame, and the first magnetic sleeve frame and the second magnetic sleeve frame are respectively movably arranged on the support frame;

the first magnetic sleeve frame and the second magnetic sleeve frame can move to the vertical direction to be arranged at intervals or move to the horizontal position.

In one possible embodiment, the sample extraction device further comprises a case, and the rack, the incubator, and the magnetic attraction mechanism are located in the case.

The application provides a sample draws equipment, through inhaling the mechanism to magnetism and designing, makes the bar magnet subassembly of inhaling the supporting rack installation of mechanism to have two kinds at least framework modes, and the bar magnet subassembly of different framework modes can adapt to the kit of different configuration modes. The appropriate architecture mode can be selected according to the requirement of sample extraction, the extraction flux of the sample extraction equipment can be increased, the sample extraction efficiency is improved, the utilization rate of the kit can be improved for samples with large requirements and simpler extraction process, the waste of consumables is avoided, and the cost of sample extraction is reduced.

Drawings

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required to be used in the description of the embodiments or the prior art are briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present application. For a person skilled in the art, without inventive effort, further figures can be obtained from these figures.

Fig. 1 is a schematic external view of a sample extraction device provided in an embodiment of the present application;

FIG. 2 is a schematic view of the sample extraction device of FIG. 1 in an open state;

FIG. 3 is a schematic diagram of the internal structure of the sample extraction device of FIG. 1;

FIG. 4a is a schematic view of a configuration of a kit provided in an embodiment of the present application;

FIG. 4b is a schematic view of another configuration of a kit provided in the examples of the present application;

fig. 5a is a schematic structural view of a magnetic attraction mechanism according to a first configuration mode provided in the present application;

fig. 5b is a schematic structural view of a magnetic attraction mechanism according to a second configuration mode provided in the present application;

fig. 6a is a schematic structural diagram of an operating mode of an incubation module provided in an embodiment of the present application;

fig. 6b is a schematic structural diagram of another operation mode of the incubation module provided in this embodiment.

Description of the reference numerals:

1-equipment;

100-a chassis; 200-a frame; 300-an incubator; 400-a magnetic attraction mechanism;

110-a box body; 120-hatch door; 210-a work bench; 310-incubation module; 410-a support frame; 420-a magnetic rod rack assembly; 430-a magnetic sleeve frame assembly;

111-a display screen; 112-an indicator light; 113-power key; 114-a loudspeaker; 115-a data interface; 116-a vent; 117-ultraviolet lamp; 118-a fan; 119-a scanner; 211-a limiting seat; 212-a first motor; 311-an incubation component; 312-a mount; 421-magnetic rod rack; 422-a magnetic bar assembly; 423-a second electric machine; 431-magnetic sleeve frame; 432-a magnetic sleeve assembly; 433-a third motor;

4221-magnetic rod; 4311-first magnetic holder; 4312-second magnetic holder; 4321-magnetic cover;

2-a kit; 201-lysis wells; 202-wash well; 203-elution well; 204-magnetic bead well.

Detailed Description

In the process of analyzing biological samples such as human organ tissues, whole blood, plasma, serum, biological fluids and the like, the samples are usually extracted and purified first, and then the extracted and purified samples are detected and analyzed. Taking nucleic acid extraction as an example, firstly, cell lysis is performed on the collected sample, nucleic acid is separated and extracted, and then, the nucleic acid is purified, so that high-purity nucleic acid is obtained.

At present, the magnetic bead method which is convenient and efficient to operate is generally adopted in the market to extract nucleic acid, a kit containing a sample is placed on a workbench of nucleic acid extraction equipment, the nucleic acid extraction equipment adsorbs the magnetic bead at the bottom of a magnetic sleeve by utilizing the motion of the magnetic rod and the magnetic sleeve, the magnetic bead is transferred in reagents in different extraction holes of the kit, the reagents are rapidly stirred by utilizing the high-frequency up-and-down movement of the magnetic rod and the magnetic sleeve, the nucleic acid extraction processes such as cell lysis, nucleic acid adsorption, washing, elution and the like are realized, and finally high-purity nucleic acid is obtained.

However, in the related art, the configuration of the nucleic acid extraction device is single, and for samples (such as throat swab samples) which are in large demand and have a simple extraction process, the nucleic acid extraction device has a low utilization rate of the kit, which easily causes waste of consumables, increases the cost of nucleic acid extraction, and thus limits the extraction flux of the nucleic acid extraction device, and has low sample extraction efficiency.

In view of this, the embodiment of the present application provides a sample extraction device, where a magnetic attraction mechanism is designed to have at least two configuration modes, the magnetic attraction mechanism is adaptable to kits of the at least two configuration modes, and a suitable configuration mode can be selected according to a sample extraction requirement, so that an extraction flux of the sample extraction device can be increased, sample extraction efficiency can be improved, for samples with large requirements and a simpler extraction process, a utilization rate of the kits can be increased, consumable waste can be avoided, and a sample extraction cost can be reduced.

In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

Fig. 1 is a schematic external view of a sample extraction device provided in an embodiment of the present application; fig. 2 is a schematic view of the sample extraction device of fig. 1 in an open state. Referring to fig. 1 and 2, in the present embodiment, a sample extraction apparatus 1 is provided, and the sample extraction apparatus 1 is used for extracting particles/molecules required in a (biological) sample, for example, the sample extraction apparatus 1 is used for extracting nucleic acid in cells, or the sample extraction apparatus 1 may also be used for extracting proteins, enzymes, and other substances in cells, which is not limited in the present embodiment. The sample collection apparatus 1 of the present embodiment will be described below by taking nucleic acid collection as an example.

The sample extraction device 1 (hereinafter referred to as a device) of the present embodiment includes a chassis 100, and a rack 200, an incubator 300, a magnetic attraction mechanism 400, and other components disposed in the chassis 100, where the rack 200 is used as a main body support structure, and the operation components such as the incubator 300 and the magnetic attraction mechanism 400 are all mounted on the rack 200. Wherein, the rack 200 can be provided with a workbench 210, the incubator 300 and the magnetic attraction mechanism 400 can be both arranged on the workbench 210, when nucleic acid extraction is carried out, a kit containing a sample is arranged on the incubator 300, the incubator 300 heats the reagent with temperature requirement in the kit, and the magnetic bead and the transfer nucleic acid are adsorbed by the movement of the magnetic attraction mechanism 400, so as to extract high-purity nucleic acid.

Through installing frame 200 in quick-witted case 100, in the extraction process, the inside comparatively confined environment that forms of quick-witted case 100, to the extraction of the pathogen of diseases such as highly pathogenic, easy-to-propagate avian influenza, novel coronavirus that appear in recent years, the sample is in quick-witted case 100, is difficult to increase the risk that testing personnel infects the virus. Of course, when taking a sample without pathogens such as virus and bacteria, the sample may be exposed to the air, and in this case, the rack 200 may also be exposed to the external environment, that is, the cabinet 100 may not be provided.

As shown in fig. 1 and 2, the chassis 100 may include a box 110 and a hatch 120. The side of the casing 110 from which the operator introduces the reagent vessels into the casing 110, placed on the work table 210, is open, which is typically the operating side of the apparatus 1. The openable cover of the hatch door 120 is arranged on the opening of the box body 110, when the hatch door 120 is opened, the operation of taking and placing the reagent box can be carried out, and in the process of extraction, the hatch door 120 can be kept closed. Illustratively, the upper end of the door 120 may be pivotally connected to the top of the chassis 100.

Referring to fig. 1, the outer surface of the box body 110 may be provided with a display screen 111 and an indicator 112, and the display screen 111 and the indicator 112 may be disposed on a side where the hatch 120 is located, for example, for an operator to operate and observe. The display screen 111 may be a touch screen, and can support a nucleic acid extraction operation in a touch manner; the indicator light 112 is used for indicating the working state of the device 1, the indicator light 112 may indicate the working state of the device 1 by displaying different colors, for example, the indicator light 112 does not light to indicate that the device 1 is turned off, idle or not running, the indicator light 112 displays green to indicate that the extraction operation is in progress, and the indicator light 112 displays red to indicate the failure of the device 1.

In addition, the housing 110 may further include a power key 113, a speaker 114, a data interface 115, and the like, wherein the operator turns on or off the device 1 by pressing the power key 113, the speaker 114 is used to emit a warning sound or an alarm sound, and the data interface 115 (e.g., a USB interface) is used for data communication between the device 1 and a server or other devices 1. The box 110 may further include a vent 116, where the vent 116 is formed by a plurality of vents arranged on a sidewall of the chassis 100, and the chassis 100 realizes convection of air inside and outside through the vent 116.

Referring to fig. 2, the housing 110 may be provided therein with a uv lamp 117, a fan 118, a scanner 119, etc., and the uv lamp 117, the fan 118, and the scanner 119 may be installed on an inner sidewall of the housing 110, for example. The ultraviolet lamp 117 is used for disinfecting and sterilizing the interior of the box body 110 after the extraction process is finished; the fan 118 is used for accelerating the air flow in the box body 110 and reducing aerosol pollution among samples of different batches; the scanner 119 is used to scan the barcode of the kit for entry of the extraction program.

Fig. 3 is a schematic view of the internal structure of the sample extraction device in fig. 1. Referring to fig. 3, the work table 210 is, for example, a bottom plate installed at the bottom of the case 110, and the incubator 300 and the magnetic attraction mechanism 400 are installed on the bottom plate. In order to improve the efficiency of nucleic acid extraction, the working platform 210 may be provided with a plurality of mounting areas, and the reagent cartridges (not shown) are arranged in the mounting areas, one mounting area corresponds to one reagent cartridge, so that the apparatus 1 can simultaneously extract samples from a plurality of reagent cartridges. Wherein, the installation area can be set in a manner of array arrangement to improve the space utilization of the workbench 210.

For example, the mounting regions may be arranged on the worktable 210 in an array, taking the X direction as the row direction and the Y direction as the column direction as the example, which shows that 2 rows and 3 columns of 6 mounting regions are arranged on the worktable 210, that is, the apparatus 1 can simultaneously extract samples in 6 reagent cartridges at a time. In other examples, the number of rows of the mounting areas arranged on the worktable 210 may also be 1 row, 3 rows, 4 rows or even more, and the number of columns of the mounting areas may also be 1 column, 2 columns, 4 columns, 5 columns or even more, depending on the volume size of the worktable 210, which is not particularly limited in this embodiment.

Corresponding to the installation areas, the incubator 300 may include a plurality of incubation modules 310, each incubation module 310 is installed in each installation area in a one-to-one correspondence, the reagent kit is placed on the incubation module 310, and the reagent kit is heated by the incubation module 310. Wherein, incubation module 310 includes a plurality of incubation subassemblies 311, heats for the required extraction hole site of kit through incubation subassembly 311 to guarantee to extract required temperature requirement.

Specifically, the workbench 210 may be provided with a limiting seat 211, the limiting seat 211 may be set to be a retaining wall structure, and an area surrounded by the retaining wall is an installation area. Incubation module 310 is correspondingly installed in the region that spacing seat 211 encloses, and the kit is placed in the region that spacing seat 211 encloses, carries out spacing fixed to the kit through spacing seat 211, and the kit is correspondingly arranged on incubation module 310.

With continued reference to fig. 3, the magnetic attachment mechanism 400 includes a support bracket 410, a magnet bar assembly 420, and a magnet sleeve assembly 430. The supporting frame 410 is movably mounted on the working platform 210, and for example, the first motor 212 may be mounted on the working platform 210, and the supporting frame 410 is driven by the first motor 212 to move along a first direction (X direction). The bar magnet assembly 420 and the magnetic sleeve assembly 430 are movably mounted on the support frame 410, the bar magnet assembly 420 may be located on a side of the magnetic sleeve assembly 430 facing away from the worktable 210, i.e. the bar magnet assembly 420 may be located above the magnetic sleeve assembly 430, and the bar magnet assembly 420 and the magnetic sleeve assembly 430 may be moved to be sleeved on or separated from each other.

The bar magnet assembly 420 includes a bar magnet frame 421 and a plurality of bar magnet assemblies 422 mounted on the bar magnet frame 421, and the bar magnet assemblies 422 are vertically disposed on a side surface of the bar magnet frame 421 facing the workbench 210. The bar magnet assembly 420 may further include a second motor 423, and the second motor 423 may be mounted on the supporting frame 410, for example, and the second motor 423 drives the bar magnet frame 421 to move vertically, so as to drive the bar magnet assembly 422 to move vertically.

Accordingly, the magnetic sleeve assembly 430 comprises a magnetic sleeve frame 431 and a plurality of magnetic sleeve assemblies 432 mounted on the magnetic sleeve frame 431, the magnetic sleeve assemblies 432 are vertically arranged on one side surface of the magnetic sleeve frame 431 facing the workbench 210, and each magnetic sleeve assembly 432 corresponds to each magnetic rod assembly 422. The magnetic sleeve assembly 430 may further include a third motor 433, and the third motor 433 may be mounted on the supporting frame 410, for example, and the third motor 433 drives the magnetic sleeve frame 431 to vertically move so as to drive the magnetic sleeve assembly 432 to vertically move.

Specifically, each magnetic rod assembly 422 may include a plurality of magnetic rods 4221 arranged in sequence, each magnetic sleeve assembly 432 may include a plurality of magnetic sleeves 4321 arranged in sequence, and each magnetic rod 4221 may be inserted into each magnetic sleeve 4321 correspondingly. In this way, each magnetic rod assembly 422 and each magnetic sleeve assembly 432 can simultaneously realize the extraction operation of a plurality of samples, and the nucleic acid extraction efficiency can be improved.

When nucleic acid extraction is performed, the magnetic rod rack assembly 420 moves downwards to be sleeved with the magnetic sleeve rack assembly 430, each magnetic rod 4221 of the magnetic rod assembly 422 is inserted into each magnetic sleeve 4321 of the corresponding magnetic sleeve assembly 432, the first motor 212 drives the support frame 410 to move along the first direction (the X direction), and the magnetic rod 4221 and the magnetic sleeve 4321 realize nucleic acid extraction operation together; after extraction is finished, the bar magnet assembly 420 moves upwards to be separated from the magnetic sleeve assembly 430, and the bar magnet assembly 422 and the magnetic sleeve assembly 432 are staggered up and down so as to be convenient for replacing the magnetic sleeve assembly 432.

In addition, for the case that the magnetic sleeve assemblies 432 on the magnetic sleeve frame 431 are arranged in multiple rows along the second direction (Y direction), in this embodiment, the magnetic sleeve frame 431 may have a separable structure, for example, as shown in fig. 3, the magnetic sleeve frame 431 may include a first magnetic sleeve frame 4311 and a second magnetic sleeve frame 4312, the first magnetic sleeve frame 4311 and the second magnetic sleeve frame 4312 are arranged in front and behind along the Y direction, and at least one row of magnetic sleeve assemblies 432 are respectively mounted on the first magnetic sleeve frame 4311 and the second magnetic sleeve frame 4312, so that the magnetic sleeve assemblies 432 can be easily mounted and dismounted, the operation of the apparatus 1 is simple, and the sample extraction efficiency is improved.

In the nucleic acid extraction process, the first magnetic sleeve holder 4311 and the second magnetic sleeve holder 4312 can be in the same horizontal position, so that the magnetic sleeve component 432 on the first magnetic sleeve holder 4311 and the magnetic sleeve component 432 on the second magnetic sleeve holder 4312 can be jointly extracted; after extraction is finished, the first magnetic sleeve holder 4311 and the second magnetic sleeve holder 4312 can be controlled to move to separate the two up and down, so that the magnetic sleeve component 432 on the first magnetic sleeve holder 4311 and the magnetic sleeve component 432 on the second magnetic sleeve holder 4312 are staggered up and down, and the magnetic sleeve component 432 can be conveniently taken out and installed.

As for the driving manner of the magnetic sleeve frame assembly 430, in some embodiments, only one third motor 433 may be provided, and the third motor 433 may drive the first magnetic sleeve frame 4311 (the second magnetic sleeve frame 4312) to move, and the first magnetic sleeve frame 4311 (the second magnetic sleeve frame 4312) drives the second magnetic sleeve frame 4312 (the first magnetic sleeve frame 4311) to move; in other embodiments, two third motors 433 may be provided, and the two third motors 433 respectively drive the first magnetic sleeve holder 4311 and the second magnetic sleeve holder 4312 to move.

In this embodiment, the magnetic attraction mechanism 400 and the incubator 300 are designed to have at least two structural modes for the magnetic attraction mechanism 400, and correspondingly, each incubation module 310 of the incubator 300 has at least two working modes for the magnetic attraction mechanism 400 and the incubator 300 to adapt to a kit having at least two configuration modes. Therefore, the configuration mode of the equipment 1 is more flexible, the extraction flux of the equipment 1 can be increased, and the extraction efficiency of the equipment 1 is improved; and, the configuration mode of the kit that matches with equipment 1 and use is also more nimble, and is higher to the utilization ratio of kit, can prevent to cause the consumptive material extravagant, reduces the sample and draws the cost.

In the magnetic attraction mechanism 400 with different structural modes, the magnetic rod assemblies 422 in the magnetic rod frame assemblies 420 are different in layout structure on the magnetic rod frame 421, the magnetic sleeve assemblies 432 in the magnetic sleeve frame assemblies 430 are different in layout structure corresponding to the magnetic rod assemblies 422, and the magnetic sleeve assemblies 432 are matched with the layout structure of the magnetic rod assemblies 422; in the incubator 300 with different operation modes, the incubation component 311 in each incubation module 310 can heat the extraction holes at different positions of the kit. The layout structure of the magnetic rod assembly 422 (magnetic sleeve assembly 432) and the working mode of the incubation module 310 are adapted to the configuration mode of the reagent kit, so that the magnetic rod assembly 422 (magnetic sleeve assembly 432) with different layout structures and the incubation module 310 with different working modes can be adapted to the reagent kits with different configuration modes.

The nucleic acid extraction process is described below with reference to the layout structure of the kit.

FIG. 4a is a schematic view of a configuration of a kit provided in an embodiment of the present application; fig. 4b is a schematic view of another configuration of the kit provided in the embodiments of the present application. Referring to FIGS. 4a and 4b, many of the conventional nucleic acid extraction kits 2 on the market are 96-well plates, in which 96 extraction wells are arrayed, 8 lines (A-H) of extraction wells are provided, and 12 extraction wells are arrayed in each line. Corresponding to each step of nucleic acid extraction, the extraction well may include a lysis well 201, a washing well 202, an elution well 203, and a magnetic bead well 204, wherein the lysis well 201, the washing well 202, and the elution well 203 are filled with corresponding extraction reagents, and the magnetic bead well 204 is filled with magnetic beads.

When extracting nucleic acid, firstly putting a sample into the cracking hole 201 for cracking, releasing the nucleic acid from cells, then enabling the magnetic rod 4221 and the magnetic sleeve 4321 to enter the magnetic bead hole 204 to adsorb magnetic beads at the bottom of the magnetic sleeve 4321, enabling the magnetic rod 4221 and the magnetic sleeve 4321 to carry the magnetic beads to be sequentially transferred in the cracking hole 201, the washing hole 202 and the elution hole 203, adsorbing the nucleic acid in the cracking hole 201 on the surface of the magnetic beads, enabling the magnetic beads adsorbed with the nucleic acid to enter the washing hole 202, washing to remove impurities such as protein and polysaccharide except the nucleic acid, and then enabling the magnetic beads to enter the elution hole 203 to dissociate the nucleic acid adsorbed on the magnetic beads, and finally obtaining the high-purity and high-concentration nucleic acid.

During extraction, incubation module 310 heats the extraction reagents in lysis well 201 and elution well 203 to provide the temperature requirements for lysis and elution. Illustratively, in conjunction with fig. 3, the incubation component 311 is disposed below the lysis well 201 and the elution well 203 to heat the extraction reagent in the lysis well 201 and the elution well 203 to meet the extraction requirement.

Continuing to refer to fig. 4a and 4b, for example, for the convenience of operation, the lysis well 201, the washing well 202, the elution well 203, and the magnetic bead well 204 required for a single sample may be arranged in sequence along the row direction of the reagent cartridge 2, and this embodiment defines all the extraction wells included in the completion of 1 sample extraction operation as 1 sample unit, that is, each sample unit includes the lysis well 201, the washing well 202, the elution well 203, and the magnetic bead well 204. The number of washing steps performed in the extraction process may be different, and the number of washing wells 202 included in each sample unit may be different, depending on the requirements of the sample, the quality of the extraction reagent, and the like.

For example, in fig. 4a, taking the number of washing times as 3 as an example, each sample unit includes 1 lysis well 201,3 washing wells 202, 1 elution well 203, and 1 magnetic bead well 204, for a 96-well plate, 12 extraction wells in each row correspond to 2 sample units, and 8 extraction wells in each row correspond to 16 sample units, that is, the 96-well plate can complete extraction of 16 samples at most; in fig. 4b, taking the number of washing times as 1 as an example, each sample unit includes 1 lysis well 201, 1 washing well 202, 1 elution well 203, and 1 magnetic bead well 204, for a 96-well plate, 12 extraction wells in each row correspond to 3 sample units, and 8 rows of extraction wells correspond to 24 sample units, that is, the 96-well plate can complete extraction of 24 samples at most.

As described above, in order to improve the extraction efficiency, a plurality of magnetic rods 4221 (magnetic sleeves 4321) are sequentially arranged to form one magnetic rod assembly 422 (magnetic sleeve assembly 432), and for the case where the extraction holes of the sample units are arranged along the row direction of the reagent kit 2, the magnetic rods 4221 (magnetic sleeves 4321) of the magnetic rod assembly 422 (magnetic sleeve assembly 432) may be arranged along the column direction of the reagent kit 2, and each magnetic rod assembly 422 (magnetic sleeve assembly 432) may perform the extraction operation on a plurality of samples at the same time. For example, each magnetic rod assembly 422 (magnetic sleeve assembly 432) may be provided with 8 magnetic rods 4221 (magnetic sleeves 4321) corresponding to the layout structure of a 96-well plate, so that one magnetic rod assembly 422 (magnetic sleeve assembly 432) may perform the extraction operation on 8 samples placed in each row of the reagent cartridge 2 at the same time.

Further, a plurality of magnetic rod assemblies 422 (magnetic sleeve assemblies 432) may be provided in the magnetic rod frame assembly 420 (magnetic rod 4221 sleeve assembly) corresponding to each sample cell arranged in the row direction of each reagent cartridge 2, one for one. Continuing with the example of the reagent kit 2 as a 96-well plate, referring to fig. 4a, for the case that each row of the reagent kit 2 includes 2 sample units, the magnetic rod rack assembly 420 (magnetic rod 4221 set assembly) may be provided with 2 magnetic rod assemblies 422 (magnetic sleeve assembly 432) corresponding to each reagent kit 2; referring to fig. 4b, for the case where each row of cartridges 2 includes 3 sample units, the magnetic rod assembly 420 (magnetic rod 4221 set assembly) may be provided with 3 magnetic rod assemblies 422 (magnetic sleeve assembly 432) for each cartridge 2.

It can be seen that, in the rod magnet frame assemblies 420 with different architectures, the number of the rod magnet assemblies 422 arranged on the rod magnet frame assembly 420 corresponding to each reagent kit 2 is different from the distance between the rod magnet assemblies 422, and in the corresponding magnetic sleeve frame assemblies 430 with different architectures, the number of the magnetic sleeve assemblies 432 arranged on the magnetic sleeve frame assembly 430 corresponding to each reagent kit 2 is different from the distance between the magnetic sleeve assemblies 432

。

Taking the example that the magnetic rod assembly 420 (magnetic sleeve assembly 430) has two structural modes, the two structural modes are defined as a first structural mode and a second structural mode respectively, the first structural mode matches the reagent kit 2 in the first configuration mode, and the second structural mode matches the reagent kit 2 in the second configuration mode.

Specifically, in the reagent cartridge 2, in the first configuration mode, M sample units may be arranged along the first direction, and the magnetic rod assembly 420 (magnetic sleeve assembly 430) in the first configuration mode corresponds to each reagent cartridge 2, and has M columns of magnetic rod assemblies 422 (magnetic sleeve assemblies 432) arranged at intervals along the first direction, the magnetic rod assemblies 422 (magnetic sleeve assemblies 432) correspond to the sample units one by one, and the interval between the magnetic rod assemblies 422 is the first interval.

In the second configuration mode, the reagent cartridge 2 may have N sample units arranged along the first direction, and the magnetic rod assembly 420 (magnetic sleeve assembly 430) in the second configuration mode corresponds to each reagent cartridge 2, and has N columns of magnetic rod assemblies 422 (magnetic sleeve assemblies 432) arranged at intervals along the first direction, the magnetic rod assemblies 422 (magnetic sleeve assemblies 432) correspond to the sample units one by one, and the distance between the magnetic rod assemblies 422 is the second distance.

The M and N are positive integers, and taking M smaller than N as an example, the number of the sample units arranged in the first direction of the reagent kit 2 in the first configuration mode is equal to or smaller than the number of the sample units arranged in the first direction of the reagent kit 2 in the second configuration mode, that is, the total number of the sample units arranged in the first configuration mode of the reagent kit 2 is smaller than the total number of the sample units arranged in the second configuration mode. At this time, the number of the extraction wells of each sample unit in the first configuration is greater than that in the second configuration, and on the premise that the numbers of the lysis wells 201, the elution wells 203, and the bead wells 204 of each sample unit are all 1, the number of the washing wells 202 of each sample unit in the first configuration is greater than that in the second configuration.

Corresponding to the arrangement of the sample units of the reagent kit 2 in the first configuration mode and the second configuration mode, the number M of the magnetic rod assemblies 422 (magnetic sleeve assemblies 432) in the first configuration mode is smaller than the number N of the magnetic rod assemblies 422 (magnetic sleeve assemblies 432) in the second configuration mode, and the first distance between the magnetic rod assemblies 422 (magnetic sleeve assemblies 432) in the first configuration mode is larger than the second distance between the magnetic rod assemblies 422 (magnetic sleeve assemblies 432) in the second configuration mode, corresponding to each reagent kit 2.

Fig. 5a is a schematic structural view of a magnetic attraction mechanism according to a first configuration mode provided in the present application; fig. 5b is a schematic structural view of a magnetic attraction mechanism according to a second configuration mode provided in the present application. Exemplarily, referring to fig. 4a and 5a, taking the reagent cartridge 2 as an example that 2 sample units are arranged in the first direction in the first configuration mode, corresponding to each reagent cartridge 2, the magnetic rod assembly 420 (magnetic sleeve assembly 430) is provided with 2 magnetic rod assemblies 422 (magnetic sleeve assemblies 432) at intervals in the first direction in the first configuration mode, and the first distance between the magnetic rod assemblies 422 (magnetic sleeve assemblies 432) is the width of 5 extraction holes; referring to fig. 4b and 5b, taking 3 sample units of the reagent cartridge 2 arranged in the first direction in the second configuration as an example, corresponding to each reagent cartridge 2, the magnetic rod assembly 420 (magnetic sleeve assembly 430) is provided with 3 magnetic rod assemblies 422 (magnetic sleeve assemblies 432) at intervals in the first direction in the second configuration, and the second distance between the magnetic rod assemblies 422 (magnetic sleeve assemblies 432) is the width of 3 extraction holes.

With respect to the arrangement of the magnetic attachment mechanism 400, in some embodiments, different magnetic wand assembly 420 may be provided to accommodate different configurations of reagent cartridges 2. Continuing with the example that the magnetic rod rack assembly 420 has the first configuration mode and the second configuration mode, two sets of magnetic rod rack assemblies 420 may be provided, wherein one set of magnetic rod rack assembly 420 is in the first configuration mode, and the other set of magnetic rod rack assembly 420 is in the second configuration mode, when the reagent kit 2 is in the first configuration mode, the magnetic rod rack assembly 420 in the first configuration mode is installed, and when the reagent kit 2 is in the second configuration mode, the magnetic rod rack assembly 420 in the second configuration mode is replaced.

In other embodiments, only one bar magnet frame assembly 420 may be provided, that is, the bar magnet frames 421 for mounting the bar magnets 4221 are uniformly arranged, and there is no need to provide different bar magnet frames 421 for different structural modes. Aiming at each reagent box 2, a plurality of installation positions are arranged on the magnetic rod frame 421, all the installation positions can meet the requirements of different framework modes at the same time, and the magnetic rod assembly 422 is detachably installed on the installation positions, so that the magnetic rod assembly 422 can be installed on the corresponding installation positions according to the requirements of different framework modes to form different framework layouts and meet the requirements of reagent boxes 2 with different configuration modes.

Through setting up unified bar magnet frame 421 that has a plurality of installation positions, only need according to the demand of different framework modes, install bar magnet subassembly 422 in corresponding installation position, the design of bar magnet frame subassembly 420 of being convenient for need not to design a plurality of bar magnet frames 421, reducible equipment 1's manufacturing cost. Moreover, when the magnetic rod assembly 420 is switched to different structural modes, the magnetic rod assembly 422 only needs to be replaced without assembling or disassembling the magnetic rod assembly 421, so that the operation efficiency is high, the assembly time can be saved, and the extraction efficiency of the equipment 1 is improved.

Similar to the arrangement of the magnetic rod rack assembly 420, in some embodiments, different magnetic rack assemblies 430 may be arranged, and the magnetic rack assemblies 430 with different structural modes may be mounted on the support frame 410 in an overall replacement mode to meet the requirements of reagent cartridges 2 with different configuration modes. In other embodiments, only one magnetic assembly 430 may be provided, and because of the non-reusable manner of the magnetic assembly 432, the magnetic assembly 432 is also typically detachably mounted (e.g., plugged) to the magnetic frame 431, thereby facilitating multiple mounting locations on the magnetic frame 431 to accommodate different configurations of reagent cartridges 2 by mounting the magnetic assembly 432 to different mounting locations.

Fig. 6a is a schematic structural diagram of an operating mode of an incubation module provided in an embodiment of the present application; fig. 6b is a schematic structural diagram of another operation mode of the incubation module provided in this embodiment. Referring to fig. 6a and 6b, as for the setting mode of the incubation module 310, in order to meet the requirement that the incubation module 310 can be adapted to reagent kits 2 with different configuration modes, in this embodiment, the incubation module 310 may be provided with a mounting seat 312, each incubation component 311 may be detachably mounted to the mounting seat 312, and the incubation module 310 is adapted to reagent kits 2 with different configuration modes by changing the combination mode of the incubation components 311.

Specifically, the operation mode of the incubation module 310 can be changed by controlling the number and the position of the incubation assemblies 311 installed in the incubation module 310. Referring to fig. 4a and 4b, continuing to take a 96-well plate as an example, for the case where 12 extraction holes are arranged in each row of the 96-well plate, fig. 4a shows that 1, 7 holes in each row are lysis holes 201,5, 11 holes are elution holes 203, and fig. 4b shows that 1,5, 9 holes in each row are lysis holes 201,3, 7, 11 holes are elution holes 203, for this, in this embodiment, 6 incubation assemblies 311 may be disposed in the mounting seat 312, each incubation assembly 311 corresponds to the extraction holes in the 1 st, 3 rd, 5 th, 7 th, 9, 11 th rows, and all the incubation assemblies 311 of each incubation module 310 may satisfy the reagent kit 2 in these two configurations.

As an embodiment, different numbers of incubation assemblies 311 can be installed in the mounting seat 312, so that the incubation module 310 can work in different modes, and thus, the requirements of the reagent kit 2 with different configurations can be met. For example, referring to fig. 4a and 6a, taking the reagent cartridge 2 as the first configuration example, the extraction holes in columns 1 and 7 of the reagent cartridge 2 are lysis holes 201, and the extraction holes in columns 5 and 11 are elution holes 203, in this case, 4 incubation assemblies 311 can be loaded into the mounting seat 312, and each incubation assembly 311 is correspondingly arranged below the extraction holes in columns 1,5, 7 and 11, respectively; referring to fig. 4b and 6b, taking the reagent cartridge 2 as the second configuration example, the extraction holes in the 1 st, 5 th and 9 th columns of the reagent cartridge 2 are lysis holes 201, and the extraction holes in the 3 rd, 7 th and 11 th columns of the reagent cartridge 2 are elution holes 203, in this case, 6 incubation assemblies 311 can be loaded into the mounting seat 312, and each incubation assembly 311 is correspondingly arranged below the extraction holes in the 1 st, 3 rd, 5 th, 7 th, 9 th and 11 th columns.

As another embodiment, in order to avoid repeatedly disassembling and assembling the incubation component 311, the incubation module 310 may also be in a state where the incubation component 311 is fully loaded all the time, and the incubation module 310 may be switched between different working modes by controlling the working state of each incubation component 311. Exemplarily, referring to fig. 6b, taking the configuration of the reagent kit 2 shown in fig. 4a as an example, the 4 incubation assemblies 311 of 1 st, 3 rd, 4 th and 6 th in the incubation module 310 may be controlled to operate, and the 2 nd incubation assemblies 311 of 2 nd and 5 th do not operate; taking the configuration of the reagent kit 2 shown in fig. 4b as an example, all of the 6 incubation assemblies 311 in the incubation module 310 may be controlled to be in the working state.

In the description of the present application, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are used merely for convenience of description and for simplicity of description, and do not indicate or imply that the referenced device or element must have a particular orientation, be constructed in a particular orientation, and be operated, and thus should not be considered as limiting the present application.

Finally, it should be noted that: the above embodiments are only used for illustrating the technical solutions of the present application, and not for limiting the same; although the present application has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art will understand that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and these modifications or substitutions do not depart from the scope of the technical solutions of the embodiments of the present application.

Claims (11)

1. A sample extraction device, comprising:

a frame having a table;

the incubator is arranged on the workbench and used for heating the kit loaded on the incubator;

the magnetic attraction mechanism comprises a support frame and a magnetic rod frame assembly, the support frame is installed on the workbench and moves along a first direction, the magnetic rod frame assembly is movably installed on the support frame, and the magnetic rod frame assembly has at least two framework modes.

2. The sample extraction apparatus of claim 1, wherein the magnetic rod assembly comprises a magnetic rod rack and a plurality of magnetic rod assemblies, the magnetic rod rack is movably mounted on the support frame, and the magnetic rod assemblies are vertically arranged on one side of the magnetic rod rack facing the reagent kit;

in the magnetic rod rack assemblies with different framework modes, the layout frameworks of the magnetic rod assemblies on the magnetic rod rack are different.

3. The sample extraction apparatus of claim 2, wherein the magnetically attractive mechanism comprises at least two sets of the magnetic rod rack assemblies, the arrangement of the magnetic rod assemblies on the magnetic rod rack assemblies being different for different magnetic rod rack assemblies.

4. The sample extraction apparatus of claim 2, wherein the magnetically attractive structure comprises one of the magnetic wand assembly, the magnetic wand assembly having a plurality of mounting locations to which the magnetic wand assembly is removably mountable;

and the magnetic rod components are different in layout structure under different structure modes.

5. The sample extraction apparatus of claim 2, wherein the magnetic wand assembly has a first architecture and a second architecture;

the magnetic rod rack assembly of the first framework mode is provided with M rows of magnetic rod assemblies which are arranged along the first direction at intervals corresponding to each reagent box, and the magnetic rod rack assembly of the second framework mode is provided with N rows of magnetic rod assemblies which are arranged along the first direction at intervals corresponding to each reagent box;

wherein M and N are positive integers, and M is less than N.

6. The sample extraction apparatus as claimed in any one of claims 1 to 5, wherein the incubator comprises a plurality of incubation modules, each of which can carry one of the kits, the incubation modules having at least two modes of operation.

7. The sample extraction apparatus of claim 6, wherein the incubation module comprises a plurality of detachably arranged incubation assemblies, each incubation assembly being arranged in sequence along the first direction;

under different working modes, the working states of the incubation assemblies in the incubation module are different, or the number of the incubation assemblies loaded in the incubation module is different.

8. The sample extraction apparatus according to any one of claims 1 to 5, wherein the magnetically attractive structure further comprises a magnetic jacket assembly movably mounted to the support frame between the incubator and the magnetic rod assembly.

9. The sample extraction apparatus of claim 8, wherein the magnetic jacket assembly comprises a magnetic jacket frame and a plurality of magnetic jacket assemblies, the magnetic jacket frame is movably mounted on the support frame, and the magnetic jacket assemblies are vertically arranged on one side of the magnetic jacket frame facing the reagent kit;

the magnetic sleeve assemblies correspond to the magnetic rod assemblies one to one, and the magnetic rod assemblies can be moved to be inserted into the magnetic sleeve assemblies.

10. The sample extraction apparatus of claim 9, wherein the magnetic sleeve mount comprises a first magnetic sleeve mount and a second magnetic sleeve mount, the first magnetic sleeve mount and the second magnetic sleeve mount each being movably mounted to the support frame;

the first magnetic sleeve frame and the second magnetic sleeve frame can move to be arranged at intervals along the vertical direction or move to be in the same horizontal position.

11. The sample extraction apparatus of any one of claims 1 to 5, further comprising a housing, wherein the rack, the incubator, and the magnetic attraction mechanism are located within the housing.

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