CN212357225U - Portable 96-hole super-smooth nanometer magnetic bead operation instrument - Google Patents
Portable 96-hole super-smooth nanometer magnetic bead operation instrument Download PDFInfo
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- CN212357225U CN212357225U CN202021400342.XU CN202021400342U CN212357225U CN 212357225 U CN212357225 U CN 212357225U CN 202021400342 U CN202021400342 U CN 202021400342U CN 212357225 U CN212357225 U CN 212357225U
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Abstract
The utility model relates to a portable 96 hole super-smooth nanometer magnetic bead operation appearance, including following part: the device comprises a base, a sample storage tank, a sample processing device and a control device, wherein a deep hole plate and two guide rails are movably arranged on the base, and a plurality of sample holes are formed in the deep hole plate; the magnetic bead stirring mechanism is arranged between the two guide rails above the deep hole plate in a sliding manner and is used for uniformly stirring the extract to be extracted in the sample hole and the magnetic beads; go up and adsorb magnetic force mechanism, the slip setting is between two guide rails that are located magnetic bead rabbling mechanism top for with magnetic bead rabbling mechanism cooperation work in order to adsorb the magnetic bead to the magnetic bead rabbling mechanism on. The device adopts the mechanical upper adsorption magnetic frame, is more convenient than the lower adsorption operation in the market at present, and does not need power supply for operation; the magnetic action adsorption surface of the magnetic rod is reduced to a small area on the cross section of the columnar bottom of the magnetic rod, so that the ineffective loss of magnetic beads is reduced, and the nucleic acid extraction efficiency is improved.
Description
Technical Field
The utility model relates to a portable 96 hole super-smooth nanometer magnetic bead operation appearance belongs to nucleic acid extraction and equips technical field.
Background
The extraction and purification of nucleic acid is an indispensable research content in the research fields of agriculture, medicine and the like, and PCR (polymerase Chain reaction), SNP (single Nucleotide polymorphism) detection, gene sequencing and gene chip in molecular biology and molecular detection technology do not need the extraction and purification of nucleic acid, so the extraction quality and purity of the nucleic acid molecules of organisms directly influence the effect of the various detection technologies. The extraction and purification of the nucleic acid are carried out by a traditional organic reagent separation and extraction method, a silica gel membrane combined extraction method and a magnetic bead adsorption and extraction method. The traditional organic reagent separation and extraction method uses a large amount of toxic and harmful reagents such as phenol, chloroform, beta-mercaptoethanol and the like, and has the disadvantages of complicated steps, time and labor waste, low yield although some toxic reagents can be avoided in the extraction by a silica gel membrane combination method, safety, no toxicity, simple operation, short time consumption, high yield and good purity. The surface of superparamagnetic nano-particles is improved and modified by a nanotechnology to prepare superparamagnetic silicon oxide nano-magnetic beads, and the magnetic beads can be specifically identified and efficiently combined with nucleic acid molecules on a microscopic interface, so that the purity and the concentration of the extracted nucleic acid are improved. The technology is the effective combination of biological science and nanometer material science, is a major breakthrough of nucleic acid extraction technology, and is highly valued and widely applied. In particular, after the Next-generation sequencing (NGS) has been developed, the magnetic bead method is widely used in library construction.
Aiming at the technology of extracting and purifying nucleic acid by a magnetic bead method, nucleic acid extracting equipment with various specifications and shapes is developed on the market at present, and the equipment comprises a simple magnetic rack, a relatively small nucleic acid extractor and a large automatic liquid workstation.
Although several magnetic racks or nucleic acid extractors for magnetic bead extraction of nucleic acids have been developed on the market, these instruments are less or less perfectly compatible with the needs of the scientific market. Summarizing, the defects of the instruments at present mainly focus on the following aspects: 1) the nucleic acid extractor has complex structure, complex extraction process and difficult operation; 2) the magnetic bar has a large adsorption surface, so that magnetic beads are not concentrated after being adsorbed and are in a large sheet shape, the ineffective loss and waste of the magnetic beads are easily caused in subsequent experiments, and the nucleic acid yield is influenced; 3) the magnetic bar of the magnetic frame is fixed and can not be replaced after demagnetization and fracture; 4) The existing magnetic frame is more suitable for a single channel or a plurality of channels, can not be matched with the existing molecular experiment, wastes a large amount of time when processing various samples, and can not realize the high-flux DNA extraction in the true sense; 5) the whole machine can not be operated all the time without stopping, and the experimental requirements can not be flexibly met.
The large automatic liquid workstation has complete functions and realizes automation, but the large automatic liquid workstation is really applied less because of high equipment operation cost.
SUMMERY OF THE UTILITY MODEL
To the outstanding problem, the utility model provides a portable 96 hole super-smooth nanometer magnetic bead operation appearance, this equipment have solved that the nucleic acid extraction appearance structure is complicated, complex operation, and it is little to extract the flux, and the magnetic rod adsorbs not concentrated, the magnetic rod can not conveniently be changed the scheduling problem.
In order to achieve the purpose, the utility model adopts the following technical proposal:
the utility model discloses the first aspect provides a portable 96 hole super-smooth nanometer magnetic bead operation appearance, including following part:
the deep hole plate is movably arranged on the base, two guide rails are arranged on the base positioned on two sides of the deep hole plate along the vertical direction, and a plurality of sample holes are formed in the deep hole plate;
the magnetic bead stirring mechanism is arranged between the two guide rails above the deep hole plate in a sliding manner and is used for uniformly stirring the extract to be extracted in the sample hole and the magnetic beads;
go up and adsorb magnetic force mechanism, the slip sets up and is being located two of magnetic bead rabbling mechanism top between the guide rail for with magnetic bead rabbling mechanism cooperation work is in order to with the magnetic bead adsorbs extremely on the magnetic bead rabbling mechanism.
The portable 96-well super-smooth nanometer magnetic bead manipulator, preferably, the magnetic bead stirring mechanism comprises:
the two first bearing sliding blocks are respectively arranged on the two guide rails in a sliding manner, and are respectively provided with a first elastic locking device and a second elastic locking device for locking the two first bearing sliding blocks at a specified position;
the magnetic sleeve fixing frame is fixedly arranged between the two first bearing sliding blocks;
and the magnetic sleeves are fixedly arranged on the magnetic sleeve fixing frame and correspond to the sample holes one by one, and the magnetic sleeves can extend into the sample holes to complete the stirring function.
The portable 96-well superparamagnetic bead manipulator preferably, the upper adsorption magnetic mechanism includes:
the two second bearing sliding blocks are respectively arranged on the two guide rails above the two first bearing sliding blocks in a sliding manner, and the two second bearing sliding blocks are respectively provided with a third elastic locking device and a fourth elastic locking device which are used for locking the two second bearing sliding blocks at specified positions;
the magnetic bar plate frame is fixedly arranged between the two second bearing sliding blocks;
the bar magnet board is fixed on the bar magnet grillage, be provided with a plurality of bar magnets on the bar magnet board, the bar magnet with the magnetic sleeve one-to-one and can stretch into to in the magnetic sleeve, with the magnetic bead adsorbs extremely the outside of magnetic sleeve.
The portable 96-hole super-smooth nanometer magnetic bead manipulator preferably further comprises a guide rail fixing plate, wherein the guide rail fixing plate is fixed at one end, far away from the base, of the two guide rails.
Portable 96 hole super-smooth nanometer magnetic bead operation appearance, preferably, the guide rail includes first optical axis guide rail and second optical axis guide rail, two first optical axis guide rail and two the second optical axis guide rail freely makes up into two, makes magnetic bead rabbling mechanism with go up absorption magnetic force mechanism can be two first optical axis guide rail and two freely slide on the second optical axis guide rail.
The portable 96-hole super-smooth nanometer magnetic bead operation instrument is characterized in that a clamping groove is preferably arranged on the base and used for clamping the deep hole plate.
The portable 96-hole super-smooth nanometer magnetic bead manipulator is characterized in that the magnetic rod is preferably detachably arranged on the magnetic rod plate.
Preferably, the magnetic action adsorption surface of the magnetic rod is limited to the cross section area of the columnar bottom of the magnetic rod.
The utility model discloses the second aspect provides an above-mentioned portable 96 hole super-smooth nanometer magnetic bead operation appearance's operating method, including following step:
a, placing the deep hole plate containing the liquid to be extracted in the sample holes on the base, adding magnetic beads into the liquid to be extracted in each sample hole, moving the magnetic bead stirring mechanism up and down to stir the magnetic beads in the liquid to be extracted until the magnetic beads are uniformly dispersed, moving the magnetic bead stirring mechanism up, standing the deep hole plate for a certain time, and finally inserting the upper adsorption magnetic force mechanism and the magnetic bead stirring mechanism into the sample holes after being nested, wherein the magnetic beads are adsorbed at the lower end of the magnetic sleeve;
b, changing the deep hole plate in the step a, placing a new deep hole plate containing a detergent in the sample hole on the base, inserting the magnetic sleeve adsorbing magnetic beads in the step a into the sample hole, moving the upper adsorption magnetic force mechanism upwards, moving the magnetic bead stirring mechanism upwards and downwards to enable the magnetic beads to be uniformly dispersed, then moving the magnetic bead stirring mechanism upwards, standing the deep hole plate for a certain time, finally inserting the upper adsorption magnetic force mechanism and the magnetic bead stirring mechanism into the sample hole after being nested together, adsorbing the magnetic beads at the lower end of the magnetic sleeve, finally moving the magnetic bead stirring mechanism and the upper adsorption magnetic force mechanism upwards, drying the magnetic beads, and finishing washing;
c, changing the deep hole plate in the step b, placing a new deep hole plate containing eluent in the sample hole on the base, inserting the magnetic sleeve adsorbing the dry magnetic beads in the step b into the sample hole, moving the upper adsorption magnetic force mechanism upwards, moving the magnetic bead stirring mechanism upwards and downwards to uniformly mix the magnetic beads and the eluent, then moving the magnetic bead stirring mechanism upwards, standing the sample hole for a certain time, finally inserting the upper adsorption magnetic force mechanism and the magnetic bead stirring mechanism into the sample hole after being nested together, adsorbing the magnetic beads at the lower end of the magnetic sleeve, moving the magnetic bead stirring mechanism and the upper adsorption magnetic force mechanism upwards, wherein the sample hole is the dissolved nucleic acid, and thus, the elution and the nucleic acid extraction are completed.
In the operation method, preferably, the solution to be extracted is a mixture of a sample supernatant and a DNA binding solution, and in this case, before the magnetic beads are added to the solution to be extracted, the magnetic bead stirring mechanism is moved up and down to stir and mix the mixture in the sample well.
The utility model discloses owing to take above technical scheme, it has following advantage:
1. the device adopts the mechanical upper adsorption magnetic frame, is more convenient than the lower adsorption operation in the market at present, and does not need power supply for operation; the magnetic rod is detachable, and is convenient to replace when the magnetic force of the magnetic rod is insufficient, so that the service life of the instrument is prolonged; in addition, the magnetic action adsorption surface of the magnetic rod is reduced to a small area on the cross section of the columnar bottom of the magnetic rod, so that the ineffective loss of magnetic beads is reduced, and the nucleic acid yield and the extraction efficiency are increased;
2. the utility model adopts a 96-hole magnetic bar plate, perfectly agrees with a 96-hole PCR plate, an 8-channel, a 12-channel or a 96-channel liquid-transferring gun used in scientific research experiments, and has high flux, so that the experimental process can reduce a plurality of processes such as replacing an EP pipe, replacing a liquid-transferring gun head, writing numbers and the like, thereby not only saving time, but also avoiding the possibility of errors and pollution; the liquid-transfering gun can flexibly adjust flux, so that the dosage of the reagent can be conveniently adjusted; the flux extraction can be operated all the time without stopping the machine, thereby really realizing flux extraction and greatly shortening the operation procedures and the operation time;
3. the device of the utility model has simple and small structure (the size is only 180mm long, 195mm wide and 270mm high), is convenient to carry and can work under various experimental conditions;
4. the utility model discloses the device still is supporting to have quick magnetic bead method nucleic acid extraction kit, has continuous operation, saves the advantage of consumptive material.
Drawings
FIG. 1 is a schematic view of the overall structure of the device of the present invention;
FIG. 2 is another schematic view of the overall structure of the device of the present invention;
FIG. 3 is a schematic side view of the device of the present invention;
FIG. 4 is a rear view of the device of the present invention;
FIG. 5 is a schematic view of the structure of the base of the device of the present invention;
FIG. 6 is a schematic structural view of a magnetic sleeve fixing frame in the device of the present invention;
fig. 7 is a schematic structural view of a magnetic bar plate and a magnetic bar plate frame in the device of the present invention;
FIG. 8 is a schematic view of a partial structure of the device of the present invention;
FIG. 9 is a schematic structural view of a guide rail fixing plate in the device of the present invention;
the respective symbols in the figure are as follows:
1-a base; 2-deep hole plate; 3-a first elastic latch; 4-a second resilient detent; 5-magnetic sleeve fixing frame; 6-magnetic sleeve; 7-a magnetic rod plate; 8-a third resilient detent; 9-a fourth resilient detent; 10-magnetic bar plate frame; 11-a first optical axis guide; 12-a second optical axis guide; 13-a first bearing block; 14-a second bearing block; 15-guide rail fixing plate.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention are clearly and completely described below, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by the ordinary person in the art without creative work belong to the protection scope of the present invention.
In the description of the present invention, it should be understood that the terms "first", "second", "third", "fourth", etc. are used to define the components, and are only used for the convenience of distinguishing the components, and if not stated otherwise, the terms do not have special meanings, and therefore, the scope of the present invention should not be construed as being limited.
In the description of the present invention, it should be understood that the terms "upper", "lower", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and therefore should not be construed as limiting the scope of the present invention.
Example one
As shown in fig. 1 to fig. 9, the present embodiment relates to a portable 96-well superparamagnetic nanoparticle manipulator, which includes the following components:
the deep hole plate 2 is movably arranged on the base 1, two guide rails are arranged on the base 1 positioned at two sides of the deep hole plate 2 along the vertical direction, and a plurality of sample holes are formed in the deep hole plate 2;
the magnetic bead stirring mechanism is arranged between the two guide rails above the deep hole plate 2 in a sliding manner and is used for uniformly stirring the extract to be extracted in the sample hole and the magnetic beads;
go up and adsorb magnetic force mechanism, the slip setting is between two guide rails that are located magnetic bead rabbling mechanism top for with magnetic bead rabbling mechanism cooperation work in order to adsorb the magnetic bead to the magnetic bead rabbling mechanism on.
In this embodiment, as shown in fig. 1, 2, and 8, the magnetic bead stirring mechanism preferably includes: the two first bearing sliding blocks 13 are respectively arranged on the two guide rails in a sliding manner, and the two first bearing sliding blocks 13 are respectively provided with a first elastic locking device 3 and a second elastic locking device 4 which are used for locking the two first bearing sliding blocks 13 at a specified position; the magnetic sleeve fixing frame 5 is fixedly arranged between the two first bearing sliding blocks 13; the magnetic sleeve 6 and the magnetic sleeves 6 are fixedly arranged on the magnetic sleeve fixing frame 5 and are in one-to-one correspondence with the sample holes, and the magnetic sleeves 6 can extend into the sample holes to complete the stirring function.
In this embodiment, preferably, as shown in fig. 1, 2 and 7, the upper attracting magnetic mechanism includes: the two second bearing sliding blocks 14 are respectively arranged on the two guide rails above the two first bearing sliding blocks 13 in a sliding manner, and the two second bearing sliding blocks 14 are respectively provided with a third elastic locking device 8 and a fourth elastic locking device 9 for locking the two second bearing sliding blocks 14 at specified positions; the magnetic bar plate frame 10 is fixedly arranged between the two second bearing sliding blocks 14; bar magnet board 7 fixes on bar magnet grillage 10, is provided with a plurality of bar magnets on the bar magnet board 7, and bar magnet and 6 one-to-one of magnetic sleeve and can stretch into to the magnetic sleeve 6 in to adsorb the magnetic bead to the outside of magnetic sleeve 6.
In this embodiment, as shown in fig. 1 and 2, it is preferable that the guide rail fixing plate 15 is further included, and the guide rail fixing plate 15 is fixed at one end of the two guide rails away from the base 1.
In this embodiment, as shown in fig. 1 and 2, the number of the guide rails is preferably four, and the guide rails are two first optical axis guide rails 11 and two second optical axis guide rails 12, and the two first optical axis guide rails 11 and the two second optical axis guide rails 12 are freely combined into two rows, so that the magnetic bead stirring mechanism and the upper attracting magnetic force mechanism can freely slide on the two first optical axis guide rails 11 and the two second optical axis guide rails 12.
In this embodiment, as shown in fig. 5, it is preferable that the deep hole plate further includes a locking groove, and the locking groove is disposed on the base 1 and used for locking the deep hole plate 2.
In the present embodiment, it is preferable that the magnetic rod be detachably provided on the magnetic rod plate 7.
In this embodiment, the magnetic attraction surface of the magnetic rod is preferably limited to the cross section area of the column-shaped bottom of the magnetic rod.
In this embodiment, preferably, the deep-well plate 2 is a 96-well deep-well plate, and the magnetic rod plate 7 is provided with 96 magnetic rods (and the magnetic rods are detachable magnetic rods) so as to perfectly match with a 96-well PCR plate, an 8-channel, a 12-channel or a 96-channel pipetting gun used in scientific research experiments, and the throughput is high, so that many processes such as changing an EP tube, changing a pipetting gun head, writing numbers and the like can be reduced in the experimental process. The device has simple and small structure (the size is only 180mm long, 195mm wide and 270mm high), is convenient to carry, can be used in places without power on such as outdoors and the like, and greatly meets the requirements of scientific research workers on field operation.
Example two
Based on the portable 96-well superparamagnetic nanoparticle manipulator provided in embodiment 1, this embodiment provides an operation method of the apparatus, which includes the following specific steps:
a place the deep hole board 2 that holds the mixture of sample supernatant and DNA binding solution in the sample hole on the operation panel base 1 that has the screens function in, loosen first elasticity locking ware 3 and second elasticity locking ware 4, will fix magnetic sleeve 6 gliding and insert in the sample hole of deep hole board 2 on magnetic sleeve mount 5, the mixture in the magnetic sleeve 6 that slides from top to bottom in the sample hole stirs the mixing, then slide magnetic sleeve 6 to suitable position on, pin magnetic sleeve 6 with first elasticity locking ware 3 and second elasticity locking ware 4. And adding a proper amount of magnetic beads into the sample hole by using a multi-channel pipette, moving the magnetic sleeve 6 downwards into the sample hole, moving up and down, and stirring until the magnetic beads are uniformly dispersed. Then sliding the magnetic sleeve 6 upwards on the magnetic rod plate 7 in a sliding manner and locking, standing the magnetic beads for 1 minute, collecting and precipitating the magnetic beads together, then simultaneously loosening the first elastic locking device 3, the second elastic locking device 4, the third elastic locking device 8 and the fourth elastic locking device 9, sliding the magnetic sleeve 6 and the magnetic rod plate 7 which are nested together downwards to a sample hole, rapidly adsorbing the magnetic beads on the cross section of the lowest end of the magnetic sleeve 6, and finally sliding the magnetic sleeve 6 and the magnetic rod plate 7 which are adsorbed with the magnetic beads upwards to a proper position for locking, thereby completing the combination step.
b, changing the deep hole plate 2 in the step a, placing a new deep hole plate 2 containing detergent in a sample hole on the base 1, sliding the magnetic sleeve 6 and the magnetic rod plate 7 down to the sample hole together, sliding the magnetic rod plate 7 up to a proper position and locking, wherein the magnetic sleeve 6 and the magnetic rod plate 7 are separated, so that no magnetic rod is adsorbed, magnetic beads are naturally dissolved in the detergent from the magnetic sleeve 6, sliding the magnetic sleeve 6 up and down to mix the magnetic beads and wash the magnetic beads, sliding the magnetic sleeve 6 up and down on the magnetic rod plate 7 and locking, the magnetic beads stand for 1 minute, simultaneously loosening the first elastic locker 3, the second elastic locker 4, the third elastic locker 8 and the fourth elastic locker 9, sliding the magnetic sleeve 6 and the magnetic rod plate 7 which are nested together down to the sample hole, and adsorbing the magnetic beads on the cross section at the lowest end of the magnetic sleeve 6 rapidly, then the magnetic sleeve 6 with the magnetic beads and the magnetic bar plate 7 are slid upwards to a proper position, the washing step is repeated once, and the magnetic beads are naturally dried for about 30 seconds to 1 minute, so that the washing step is completed.
c, changing the deep hole plate 2 in the step b, placing a new deep hole plate 2 containing the eluent in the sample hole on the base 1, sliding the magnetic sleeve 6 with the dry magnetic beads in the step b and the magnetic rod plate 7 down to the sample hole together, sliding the magnetic rod plate 7 up to a proper position and locking, wherein the magnetic sleeve 6 and the magnetic rod plate 7 are separated without the adsorption of the magnetic rod, the magnetic beads are naturally dissolved in the eluent from the magnetic sleeve 6, sliding the magnetic sleeve 6 up and down to fully mix the magnetic beads with the eluent, the nucleic acid adsorbed on the magnetic beads is eluted, sliding the magnetic sleeve 6 up to the magnetic rod plate 7 and locking, standing the magnetic beads for 1 minute, simultaneously loosening the first elastic locker 3, the second elastic locker 4, the third elastic locker 8 and the fourth elastic locker 9, sliding the magnetic sleeve 6 and the magnetic rod plate 7 nested together down to the sample hole, after the magnetic beads are adsorbed on the magnetic sleeve 6, the magnetic sleeve 6 with the magnetic beads and the magnetic rod plate 7 slide upwards to a proper position and are locked, nucleic acid is dissolved in the sample hole, the elution step is completed, and the nucleic acid extraction is completed.
In this embodiment, a specific example is given to demonstrate the efficiency of the device of the present invention:
in the agricultural field, a large number of genetic groups, mutants and the like, even tens of thousands of group samples need to be extracted and purified, if a conventional organic solvent separation or silica gel membrane combined extraction method is used, 100 samples are extracted by one person at a medium and fast speed for 1 day, and about 100 days are required for 1 person extracting 1 ten thousand samples. If the device of the utility model is used to cooperate with the corresponding extraction kit, the extraction can be completed only in about 10 days, that is to say, 800 and 1000 samples can be extracted in 1 day at a medium speed of 1 person, and the extraction can be completed in about 10 days, so that the speed is 10 times higher than that of the traditional method, and the purity is higher than that of the traditional method.
In this embodiment, the deep hole plate 2 can be matched with 8, 12 or 96 commercially available pipette guns, and the range of the pipette gun can be flexibly adjusted, so that the reagent dosage can be flexibly adjusted through the plurality of pipette guns, and the method is convenient, rapid and time-saving.
In the embodiment, the magnetic action surface is reduced to a small area of the cross section of the lower end of the magnetic rod, the magnetic force is strong, and the magnetic sleeve 6 is sleeved with the magnetic rod and still has the adsorption capacity, so that the magnetic beads are adsorbed to the cross section of the lowest end of the magnetic sleeve 6, the pollution of the magnetic rod is avoided, and the magnetic rod is easy to wash and elute.
In this embodiment, the bar magnet is removable formula, if long-time operation, makes the magnetic force of bar magnet not enough, can be with the change that bar magnet force is not enough, this utility model device of very big increase life.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention in its corresponding aspects.
Claims (8)
1. The utility model provides a portable 96 hole super-smooth nanometer magnetic bead operation appearance which characterized in that includes following part:
the deep hole plate (2) is movably arranged on the base (1), two guide rails are arranged on the base (1) on two sides of the deep hole plate (2) along the vertical direction, and a plurality of sample holes are formed in the deep hole plate (2);
the magnetic bead stirring mechanism is arranged between the two guide rails above the deep hole plate (2) in a sliding manner and is used for uniformly stirring the extract to be extracted in the sample hole and the magnetic beads;
go up and adsorb magnetic force mechanism, the slip sets up and is being located two of magnetic bead rabbling mechanism top between the guide rail for with magnetic bead rabbling mechanism cooperation work is in order to with the magnetic bead adsorbs extremely on the magnetic bead rabbling mechanism.
2. The portable 96-well superparamagnetic bead manipulator according to claim 1, wherein said bead-stirring mechanism comprises:
the two first bearing sliding blocks (13) are respectively arranged on the two guide rails in a sliding manner, and the two first bearing sliding blocks (13) are respectively provided with a first elastic locking device (3) and a second elastic locking device (4) which are used for locking the two first bearing sliding blocks (13) at a specified position;
the magnetic sleeve fixing frame (5) is fixedly arranged between the two first bearing sliding blocks (13);
the magnetic sleeve fixing frame comprises magnetic sleeves (6), wherein the magnetic sleeves (6) are fixedly arranged on the magnetic sleeve fixing frame (5) and correspond to the sample holes one by one, and the magnetic sleeves (6) can extend into the sample holes to complete the stirring function.
3. The portable 96-well superparamagnetic bead manipulator according to claim 2, wherein said upper attracting magnetic mechanism comprises:
the two second bearing sliding blocks (14) are respectively arranged on the two guide rails above the two first bearing sliding blocks (13) in a sliding manner, and the two second bearing sliding blocks (14) are respectively provided with a third elastic locking device (8) and a fourth elastic locking device (9) for locking the two second bearing sliding blocks (14) at a specified position;
the magnetic bar plate frame (10) is fixedly arranged between the two second bearing sliding blocks (14);
bar magnet board (7) are fixed on bar magnet grillage (10), be provided with a plurality of bar magnets on bar magnet board (7), the bar magnet with magnetic sleeve pipe (6) one-to-one and can stretch into to in magnetic sleeve pipe (6), with will the magnetic bead adsorbs extremely the outside of magnetic sleeve pipe (6).
4. The portable 96-well superparamagnetic bead operator according to claim 2, further comprising a rail fixing plate (15), wherein said rail fixing plate (15) is fixed to an end of said two rails away from said base (1).
5. The portable 96-well superparamagnetic nano-bead manipulator according to claim 2, wherein said guides comprise a first optical axis guide (11) and a second optical axis guide (12), and two of said first optical axis guide (11) and two of said second optical axis guide (12) are freely combined into two rows, so that said bead stirring mechanism and said upper attracting magnetic mechanism can freely slide on two of said first optical axis guide (11) and two of said second optical axis guide (12).
6. The portable 96-well superparamagnetic bead manipulator according to claim 2, characterized in that a clamping groove is provided on said base (1), said clamping groove is used for clamping said deep well plate (2).
7. The portable 96-well superparamagnetic bead operator according to claim 3, wherein said magnetic rod is detachably provided on said magnetic rod plate (7).
8. The portable 96-well superparamagnetic nano-bead manipulator according to claim 3, wherein a magnetic action absorption surface of said magnetic rod is limited to a region of a cross section of a column-shaped bottom of said magnetic rod.
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| CN202021400342.XU CN212357225U (en) | 2020-07-16 | 2020-07-16 | Portable 96-hole super-smooth nanometer magnetic bead operation instrument |
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| CN202021400342.XU CN212357225U (en) | 2020-07-16 | 2020-07-16 | Portable 96-hole super-smooth nanometer magnetic bead operation instrument |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111690518A (en) * | 2020-07-16 | 2020-09-22 | 中国农业科学院生物技术研究所 | High-flux portable nucleic acid extractor based on magnetic bead method and operation method thereof |
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2020
- 2020-07-16 CN CN202021400342.XU patent/CN212357225U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111690518A (en) * | 2020-07-16 | 2020-09-22 | 中国农业科学院生物技术研究所 | High-flux portable nucleic acid extractor based on magnetic bead method and operation method thereof |
| CN111690518B (en) * | 2020-07-16 | 2023-11-21 | 中国农业科学院生物技术研究所 | Portable 96 Kong Chaoshun nanometer magnetic bead operating instrument and operating method thereof |
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| GR01 | Patent grant | ||
| GR01 | Patent grant |